pycom13g09510

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000852
Physical Location & Seq
Forward (+)
6379836 .. 6381144
1309 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g09510.2

Sequence Viewer

Length: 1269 bp
ATGAAGGTTGAAGTTGTAGTAATCTCCAAGGAGATTATCAGACCATCTTCTCCAACTCCTGATCACCTTCGCACATACCAGCTTTCCTTTCTCGACCAGATATCTCCTCCGGTCTATAATCCTTTGGTCATATTCTATTCCTCATCAGAGCCCGAAACAAAATTCGATATTGTTGAAATATCCAATCAACTTAAGAAATCCCTATCCAATGTCTTGACTCAATTCTACCCGCTAGCCGGACGTGCCCAAGGCAACTTGTTCATAGACTGCAATGACCAGGGAATACCCTACTTGGAAACTAGAGTTAAGTGCCAACTTTCTGATGTTATTCAAAACCCAATTCCTATCGAACTCAACAAATTCATTCCATTCCAAGTAGATGAAGTTGCTGATATTTGTTTCGGTGTCCAACTCAACATCTTTGAGTGTGGTGGCATTGCTCTCGGTATGTGCATTTCCCATAAGATTGCGGACGCATTATCGTTTTTCAATTTCATCAAAACTTGGGCAGCCACTGCTAGACAACAAGGTGATCGATATAGTAACATATCTGGGTTTAATCCAAGAATTGGCATCACAAGGGAGAACATTGTAACCAGAATGTTTGTCTTTGAGGCTTCAAAGATAGAGGCTCTCAAAACAAAATATGGTGCTGGTGATCACAGCAAGAGCTTAGAAAATCACCAAAAGCCGCCTTCTCGTGTGGAGGCTTTATCCGCTTTCATATGGGACCGCTTCATGGCTGCCACTCAGGTGAAGTCAGACATTGCACCAAAGAGGTTCTACGCCATTGTCCATGCAGTAAACCTTCGTACGAGGATTGATCCGCCATTGCCGGAACACTCATTTGGAAATCTATACAGAATTGCAATGACAATCCCGTCTTTGGATACCGGGGAGGAATGCTATGGCCTAGTGAGGCAGGTGAGAGACCAAGTGAAGAAAATCGACAAAAACTACGTTGAAAAATTGCAGAAGGGCAGTGAGCATTTGGATTTCATCAAACAGCGCTCCGATAATGTCTTCAAAGGAGAGATGGTAACGATGAGCTTCACCAGCCTGTGTAGGTTTCCACTGTATGAAGCTGATTTTGGATGGGGAAACCCTACTTGGGTTGGCTCGCCAGCCCTAACTTTCAATAATTTGGTTGTTTTCATCGACGCTAAGTCTGGTGGTGGGATTGAAGCATACATTAACATGAAGGAAGAAGACTTGGATAAAATTGAAGGCGACGGTGAGTTCCTTGCCTTTGTTTCTCCAAATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

423

Amino Acids

47.58

Weight (kDa)

5.9

Isoelectric Point (pI)

33.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 913
AasI GACNNNNNNGTC 1 cut(s) 880
Acc36I ACCTGC 1 cut(s) 913
AccB7I CCANNNNNTGG 1 cut(s) 569
AciI CCGC 6 cut(s) 230, 470, 692, 717, 733, 827
AclWI GGATC 1 cut(s) 818
AcsI RAATTY 2 cut(s) 161, 359
AfaI GTAC 1 cut(s) 814
AfeI AGCGCT 1 cut(s) 1010
AfiI CCNNNNNNNGG 5 cut(s) 236, 569, 739, 886, 1111
AflII CTTAAG 1 cut(s) 191
AhdI GACNNNNNGTC 1 cut(s) 1165
AjiI CACGTC 1 cut(s) 242
AjnI CCWGG 1 cut(s) 276
AjuI GAANNNNNNNTTGG 6 cut(s) 831, 863, 1074, 1093, 1106, 1125
AleI CACNNNNGTG 1 cut(s) 752
AluBI AGCT 4 cut(s) 82, 672, 1050, 1085
AluI AGCT 4 cut(s) 82, 672, 1050, 1085
Alw26I GTCTC 1 cut(s) 924
AlwI GGATC 1 cut(s) 818
AlwNI CAGNNNCTG 1 cut(s) 515
Aor51HI AGCGCT 1 cut(s) 1010
AoxI GGCC 1 cut(s) 910
ApeKI GCWGC 2 cut(s) 509, 743
ApoI RAATTY 2 cut(s) 161, 359
AspLEI GCGC 1 cut(s) 1011
AspS9I GGNCC 1 cut(s) 730
AsuC2I CCSGG 1 cut(s) 895
AsuHPI GGTGA 8 cut(s) 56, 542, 668, 674, 766, 937, 1045, 1247
AsuNHI GCTAGC 1 cut(s) 232
AvaII GGWCC 1 cut(s) 730
BaeGI GKGCMC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 153
BauI CACGAG 1 cut(s) 699
BbsI GAAGAC 2 cut(s) 1015, 1215
BbvI GCAGC 2 cut(s) 521, 730
BccI CCATC 3 cut(s) 52, 1030, 1089
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BciT130I CCWGG 1 cut(s) 278
BciVI GTATCC 1 cut(s) 883
BclI TGATCA 2 cut(s) 61, 658
BcnI CCSGG 1 cut(s) 895
BcoDI GTCTC 1 cut(s) 924
BfaI CTAG 4 cut(s) 233, 300, 519, 914
BfoI RGCGCY 1 cut(s) 1012
BfrI CTTAAG 1 cut(s) 191
BfuAI ACCTGC 1 cut(s) 913
BfuI GTATCC 1 cut(s) 883
BisI GCNGC 3 cut(s) 510, 692, 744
BlsI GCNGC 3 cut(s) 511, 693, 745
Bme1390I CCNGG 2 cut(s) 278, 895
Bme18I GGWCC 1 cut(s) 730
BmeRI GACNNNNNGTC 1 cut(s) 1165
BmgBI CACGTC 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 730
BmiI GGNNCC 1 cut(s) 731
BmrFI CCNGG 2 cut(s) 278, 895
BmsI GCATC 1 cut(s) 582
BmtI GCTAGC 1 cut(s) 236
BpiI GAAGAC 2 cut(s) 1015, 1215
BpuMI CCSGG 1 cut(s) 895
Bsa29I ATCGAT 1 cut(s) 535
BsaI GGTCTC 1 cut(s) 924
BsaJI CCNNGG 4 cut(s) 27, 247, 277, 894
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 1023, 1053
Bsc4I CCNNNNNNNGG 5 cut(s) 236, 569, 739, 886, 1111
Bse3DI GCAATG 5 cut(s) 277, 435, 765, 830, 876
BseBI CCWGG 1 cut(s) 278
BseCI ATCGAT 1 cut(s) 535
BseDI CCNNGG 4 cut(s) 27, 247, 277, 894
BseGI GGATG 1 cut(s) 1100
BseLI CCNNNNNNNGG 5 cut(s) 236, 569, 739, 886, 1111
BseMI GCAATG 5 cut(s) 277, 435, 765, 830, 876
BseMII CTCAG 1 cut(s) 764
BseRI GAGGAG 1 cut(s) 96
BseSI GKGCMC 1 cut(s) 247
BseXI GCAGC 2 cut(s) 521, 730
BshFI GGCC 1 cut(s) 912
BshVI ATCGAT 1 cut(s) 535
BsiSI CCGG 4 cut(s) 110, 237, 836, 894
BsiWI CGTACG 1 cut(s) 812
BslFI GGGAC 1 cut(s) 743
BslI CCNNNNNNNGG 5 cut(s) 236, 569, 739, 886, 1111
BsmAI GTCTC 1 cut(s) 924
BsmFI GGGAC 1 cut(s) 743
BsmI GAATGC 1 cut(s) 908
BsnI GGCC 1 cut(s) 912
Bso31I GGTCTC 1 cut(s) 924
Bsp1286I GDGCHC 2 cut(s) 153, 247
Bsp143I GATC 4 cut(s) 61, 532, 658, 823
BspACI CCGC 6 cut(s) 230, 470, 692, 717, 733, 827
BspANI GGCC 1 cut(s) 912
BspCNI CTCAG 1 cut(s) 763
BspDI ATCGAT 1 cut(s) 535
BspLI GGNNCC 1 cut(s) 731
BspMI ACCTGC 1 cut(s) 913
BspOI GCTAGC 1 cut(s) 236
BspPI GGATC 1 cut(s) 818
BspTI CTTAAG 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 924
BsrDI GCAATG 5 cut(s) 277, 435, 765, 830, 876
BssECI CCNNGG 4 cut(s) 27, 247, 277, 894
BssMI GATC 4 cut(s) 61, 532, 658, 823
BssSI CACGAG 1 cut(s) 699
BssT1I CCWWGG 2 cut(s) 27, 247
Bst2BI CACGAG 1 cut(s) 699
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 2 cut(s) 1077, 1235
BstAFI CTTAAG 1 cut(s) 191
BstAPI GCANNNNNTGC 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 234, 1121, 1125
BstDEI CTNAG 3 cut(s) 673, 750, 1164
BstF5I GGATG 1 cut(s) 1100
BstH2I RGCGCY 1 cut(s) 1012
BstHHI GCGC 1 cut(s) 1011
BstKTI GATC 4 cut(s) 64, 535, 661, 826
BstMAI GTCTC 1 cut(s) 924
BstMBI GATC 4 cut(s) 61, 532, 658, 823
BstMWI GCNNNNNNNGC 4 cut(s) 242, 515, 716, 1056
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 2 cut(s) 276, 893
BstSLI GKGCMC 1 cut(s) 247
BstV1I GCAGC 2 cut(s) 521, 730
BstV2I GAAGAC 2 cut(s) 1015, 1215
Bsu15I ATCGAT 1 cut(s) 535
BsuI GTATCC 1 cut(s) 883
BsuRI GGCC 1 cut(s) 912
BsuTUI ATCGAT 1 cut(s) 535
BtrI CACGTC 1 cut(s) 242
BtsCI GGATG 1 cut(s) 1100
BtsI GCAGTG 2 cut(s) 513, 988
BtsIMutI CAGTG 3 cut(s) 513, 988, 1073
BveI ACCTGC 1 cut(s) 913
Cac8I GCNNGC 3 cut(s) 234, 1121, 1125
CaiI CAGNNNCTG 1 cut(s) 515
CfoI GCGC 1 cut(s) 1011
Cfr13I GGNCC 1 cut(s) 730
ClaI ATCGAT 1 cut(s) 535
CseI GACGC 2 cut(s) 482, 1169
Csp6I GTAC 1 cut(s) 813
CviAII CATG 3 cut(s) 739, 797, 1198
CviQI GTAC 1 cut(s) 813
DdeI CTNAG 3 cut(s) 673, 750, 1164
DpnI GATC 4 cut(s) 63, 534, 660, 825
DpnII GATC 4 cut(s) 61, 532, 658, 823
DrdI GACNNNNNNGTC 1 cut(s) 880
DriI GACNNNNNGTC 1 cut(s) 1165
DseDI GACNNNNNNGTC 1 cut(s) 880
Eam1105I GACNNNNNGTC 1 cut(s) 1165
EciI GGCGGA 1 cut(s) 816
Eco130I CCWWGG 2 cut(s) 27, 247
Eco24I GRGCYC 1 cut(s) 153
Eco31I GGTCTC 1 cut(s) 924
Eco32I GATATC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 730
Eco47III AGCGCT 1 cut(s) 1010
EcoRII CCWGG 1 cut(s) 276
EcoRV GATATC 1 cut(s) 102
EcoT14I CCWWGG 2 cut(s) 27, 247
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 2 cut(s) 27, 247
FaeI CATG 3 cut(s) 742, 800, 1201
FaqI GGGAC 1 cut(s) 743
FatI CATG 3 cut(s) 738, 796, 1197
FauI CCCGC 1 cut(s) 237
FauNDI CATATG 1 cut(s) 725
FbaI TGATCA 2 cut(s) 61, 658
Fnu4HI GCNGC 3 cut(s) 510, 692, 744
FokI GGATG 1 cut(s) 1107
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 3 cut(s) 510, 692, 744
FspBI CTAG 4 cut(s) 233, 300, 519, 914
GlaI GCGC 1 cut(s) 1010
GluI GCNGC 3 cut(s) 510, 692, 744
HaeII RGCGCY 1 cut(s) 1012
HaeIII GGCC 1 cut(s) 912
HapII CCGG 4 cut(s) 110, 237, 836, 894
HgaI GACGC 2 cut(s) 482, 1169
HhaI GCGC 1 cut(s) 1011
Hin1II CATG 3 cut(s) 742, 800, 1201
Hin6I GCGC 1 cut(s) 1009
HinP1I GCGC 1 cut(s) 1009
HinfI GANTC 1 cut(s) 217
HpaII CCGG 4 cut(s) 110, 237, 836, 894
HphI GGTGA 8 cut(s) 56, 542, 668, 674, 766, 937, 1045, 1247
Hpy166II GTNNAC 1 cut(s) 805
Hpy188I TCNGA 5 cut(s) 41, 148, 322, 763, 1015
Hpy188III TCNNGA 3 cut(s) 59, 92, 214
Hpy8I GTNNAC 1 cut(s) 805
Hpy99I CGWCG 2 cut(s) 1163, 1235
HpyAV CCTTC 6 cut(s) 77, 705, 818, 970, 1195, 1220
HpyCH4III ACNGT 2 cut(s) 1077, 1235
HpyCH4IV ACGT 2 cut(s) 241, 960
HpyCH4V TGCA 6 cut(s) 270, 453, 770, 800, 869, 973
HpyF10VI GCNNNNNNNGC 4 cut(s) 242, 515, 716, 1056
HpyF3I CTNAG 3 cut(s) 673, 750, 1164
HpySE526I ACGT 2 cut(s) 241, 960
Hsp92II CATG 3 cut(s) 742, 800, 1201
HspAI GCGC 1 cut(s) 1009
Ksp22I TGATCA 2 cut(s) 61, 658
Kzo9I GATC 4 cut(s) 61, 532, 658, 823
LmnI GCTCC 1 cut(s) 1016
Lsp1109I GCAGC 2 cut(s) 521, 730
LweI GCATC 1 cut(s) 582
MaeI CTAG 4 cut(s) 233, 300, 519, 914
MaeII ACGT 2 cut(s) 241, 960
MaeIII GTNAC 3 cut(s) 542, 592, 1039
MalI GATC 4 cut(s) 63, 534, 660, 825
MboI GATC 4 cut(s) 61, 532, 658, 823
MboII GAAGA 5 cut(s) 39, 952, 1015, 1217, 1220
MhlI GDGCHC 2 cut(s) 153, 247
MlyI GAGTC 1 cut(s) 211
MmeI TCCRAC 2 cut(s) 77, 433
MnlI CCTC 9 cut(s) 117, 151, 607, 622, 700, 771, 810, 892, 912
MseI TTAA 4 cut(s) 192, 306, 558, 1194
MslI CAYNNNNRTG 2 cut(s) 752, 1196
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 4 cut(s) 110, 237, 836, 894
MspR9I CCNGG 2 cut(s) 278, 895
Mva1269I GAATGC 1 cut(s) 908
MvaI CCWGG 1 cut(s) 278
MwoI GCNNNNNNNGC 4 cut(s) 242, 515, 716, 1056
NciI CCSGG 1 cut(s) 895
NdeI CATATG 1 cut(s) 725
NdeII GATC 4 cut(s) 61, 532, 658, 823
NheI GCTAGC 1 cut(s) 232
NlaIII CATG 3 cut(s) 742, 800, 1201
NlaIV GGNNCC 1 cut(s) 731
OliI CACNNNNGTG 1 cut(s) 752
PaqCI CACCTGC 1 cut(s) 913
PctI GAATGC 1 cut(s) 908
Pfl23II CGTACG 1 cut(s) 812
PflMI CCANNNNNTGG 1 cut(s) 569
PkrI GCNGC 3 cut(s) 511, 693, 745
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspLI CGTACG 1 cut(s) 812
PspN4I GGNNCC 1 cut(s) 731
PspPI GGNCC 1 cut(s) 730
PstNI CAGNNNCTG 1 cut(s) 515
RsaI GTAC 1 cut(s) 814
RsaNI GTAC 1 cut(s) 813
RseI CAYNNNNRTG 2 cut(s) 752, 1196
SaqAI TTAA 4 cut(s) 192, 306, 558, 1194
SatI GCNGC 3 cut(s) 510, 692, 744
Sau3AI GATC 4 cut(s) 61, 532, 658, 823
Sau96I GGNCC 1 cut(s) 730
SchI GAGTC 1 cut(s) 211
ScrFI CCNGG 2 cut(s) 278, 895
SduI GDGCHC 2 cut(s) 153, 247
SfaNI GCATC 1 cut(s) 582
SinI GGWCC 1 cut(s) 730
SmiMI CAYNNNNRTG 2 cut(s) 752, 1196
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
SsiI CCGC 6 cut(s) 230, 470, 692, 717, 733, 827
SspMI CTAG 4 cut(s) 233, 300, 519, 914
StyD4I CCNGG 2 cut(s) 276, 893
StyI CCWWGG 2 cut(s) 27, 247
TaaI ACNGT 2 cut(s) 1077, 1235
TaiI ACGT 2 cut(s) 244, 963
TaqI TCGA 6 cut(s) 93, 165, 348, 535, 948, 1158
TauI GCSGC 1 cut(s) 694
Tru1I TTAA 4 cut(s) 192, 306, 558, 1194
Tru9I TTAA 4 cut(s) 192, 306, 558, 1194
TscAI CASTG 3 cut(s) 520, 988, 1080
TseI GCWGC 2 cut(s) 509, 743
TspRI CASTG 3 cut(s) 520, 988, 1080
Van91I CCANNNNNTGG 1 cut(s) 569
Vha464I CTTAAG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 730
XapI RAATTY 2 cut(s) 161, 359
XspI CTAG 4 cut(s) 233, 300, 519, 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.