Rmu_co8463717.1_g000001

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8463717.1
OG0000852
Physical Location & Seq
Forward (+)
1 .. 734
734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8463717.1_g000001.1.cds

Sequence Viewer

Length: 734 bp
tccaacaccgcaccgggatcataaagcaaaacatctcaacaaaacgcttcgtcttcgacgctccaatggttcaggccatccaagccaaatgcatggacatgtccagaagccccactcgtgttgaagccctatcagccttcatatggagccgctacataacatcatgcaccggcattcaacaagatggcgtcaccaccgacgcgatgtacactgtttctcatgctgtgaacctccgcaggaggatggagccaccacttcccgagtacacattcggaaacgtgatccaaaccaccattgcggtgccgcacatacccgttgatccgaaagatgggacgcttcatggggtggttaatcacatcagggaggccattaagcaagtagacaaagagtacatcaagaaattgcaagagggtgacggacacttgagcttcttgaggagccgtgcggaggaggtgaaacgcggagaggtggtgtcgtttaacttcacgagtctgtgtaggtttccgatctacgacgctgattttggatgggggaagcccgtttatgttggttctgccagctttacgttcaagaacttggttagtttctttgacaccaacgtaggggacggagttgaggcgtggatcaatctgttggaggaagacatggaaaaattcgaggcggacgaagagcttctcaagtatgtttcacccgccccgagttcaaagaatgccaaggcctcgattaattactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

243

Amino Acids

27.41

Weight (kDa)

6.35

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 300
AccI GTMKAC 1 cut(s) 380
AccII CGCG 2 cut(s) 202, 461
AciI CCGC 9 cut(s) 9, 150, 234, 298, 304, 445, 461, 661, 692
AclWI GGATC 4 cut(s) 25, 276, 313, 631
AcsI RAATTY 1 cut(s) 652
AcyI GRCGYC 1 cut(s) 188
AfaI GTAC 3 cut(s) 208, 265, 391
AfiI CCNNNNNNNGG 4 cut(s) 143, 328, 447, 602
AflIII ACRYGT 1 cut(s) 98
AgsI TTSAA 4 cut(s) 124, 178, 570, 704
AluBI AGCT 3 cut(s) 428, 560, 672
AluI AGCT 3 cut(s) 428, 560, 672
AlwI GGATC 4 cut(s) 25, 276, 313, 631
Ama87I CYCGRG 2 cut(s) 259, 696
AoxI GGCC 3 cut(s) 74, 365, 716
ApoI RAATTY 1 cut(s) 652
ArsI GACNNNNNNTTYG 2 cut(s) 505, 537
AseI ATTAAT 1 cut(s) 725
Asp700I GAANNNNTTC 1 cut(s) 671
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 4 cut(s) 183, 424, 465, 680
AvaI CYCGRG 2 cut(s) 259, 696
BanI GGYRCC 1 cut(s) 300
BauI CACGAG 2 cut(s) 116, 486
BbsI GAAGAC 2 cut(s) 45, 647
BccI CCATC 5 cut(s) 85, 178, 237, 322, 521
BceAI ACGGC 1 cut(s) 425
BcnI CCSGG 1 cut(s) 15
BisI GCNGC 2 cut(s) 150, 304
BlsI GCNGC 2 cut(s) 151, 305
Bme1390I CCNGG 1 cut(s) 15
BmeT110I CYCGRG 2 cut(s) 259, 696
BmiI GGNNCC 4 cut(s) 148, 248, 302, 439
BmrFI CCNGG 1 cut(s) 15
BpiI GAAGAC 2 cut(s) 45, 647
BpuEI CTTGAG 3 cut(s) 444, 453, 661
BpuMI CCSGG 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 713
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 328, 447, 602
Bse118I RCCGGY 1 cut(s) 169
Bse3DI GCAATG 1 cut(s) 293
BseDI CCNNGG 1 cut(s) 713
BseGI GGATG 3 cut(s) 77, 248, 532
BseLI CCNNNNNNNGG 4 cut(s) 143, 328, 447, 602
BseMI GCAATG 1 cut(s) 293
BseRI GAGGAG 2 cut(s) 450, 463
Bsh1236I CGCG 2 cut(s) 202, 461
BshFI GGCC 3 cut(s) 76, 367, 718
BshNI GGYRCC 1 cut(s) 300
BsiHKCI CYCGRG 2 cut(s) 259, 696
BsiSI CCGG 2 cut(s) 14, 170
BslFI GGGAC 2 cut(s) 345, 619
BslI CCNNNNNNNGG 4 cut(s) 143, 328, 447, 602
BsmFI GGGAC 2 cut(s) 345, 619
BsmI GAATGC 2 cut(s) 173, 714
BsnI GGCC 3 cut(s) 76, 367, 718
BsoBI CYCGRG 2 cut(s) 259, 696
Bsp1407I TGTACA 1 cut(s) 206
Bsp143I GATC 5 cut(s) 17, 281, 318, 506, 623
BspACI CCGC 9 cut(s) 9, 150, 234, 298, 304, 445, 461, 661, 692
BspANI GGCC 3 cut(s) 76, 367, 718
BspFNI CGCG 2 cut(s) 202, 461
BspLI GGNNCC 4 cut(s) 148, 248, 302, 439
BspPI GGATC 4 cut(s) 25, 276, 313, 631
BspQI GCTCTTC 1 cut(s) 662
BspT107I GGYRCC 1 cut(s) 300
BsrDI GCAATG 1 cut(s) 293
BsrFI RCCGGY 1 cut(s) 169
BsrGI TGTACA 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 713
BssMI GATC 5 cut(s) 17, 281, 318, 506, 623
BssNI GRCGYC 1 cut(s) 188
BssSI CACGAG 2 cut(s) 116, 486
BssT1I CCWWGG 1 cut(s) 713
Bst2BI CACGAG 2 cut(s) 116, 486
Bst4CI ACNGT 1 cut(s) 213
Bst6I CTCTTC 1 cut(s) 662
BstACI GRCGYC 1 cut(s) 188
BstAUI TGTACA 1 cut(s) 206
BstC8I GCNNGC 1 cut(s) 558
BstF5I GGATG 3 cut(s) 77, 248, 532
BstFNI CGCG 2 cut(s) 202, 461
BstKTI GATC 5 cut(s) 20, 284, 321, 509, 626
BstMBI GATC 5 cut(s) 17, 281, 318, 506, 623
BstMWI GCNNNNNNNGC 2 cut(s) 82, 133
BstNSI RCATGY 1 cut(s) 102
BstSCI CCNGG 1 cut(s) 13
BstUI CGCG 2 cut(s) 202, 461
BstV2I GAAGAC 2 cut(s) 45, 647
BstXI CCANNNNNNTGG 1 cut(s) 93
BsuRI GGCC 3 cut(s) 76, 367, 718
BtgZI GCGATG 1 cut(s) 217
BtsCI GGATG 3 cut(s) 77, 248, 532
BtsIMutI CAGTG 1 cut(s) 209
Cac8I GCNNGC 1 cut(s) 558
Cfr10I RCCGGY 1 cut(s) 169
CseI GACGC 5 cut(s) 67, 177, 208, 342, 523
Csp6I GTAC 3 cut(s) 207, 264, 390
CspCI CAANNNNNGTGG 2 cut(s) 102, 137
CviAII CATG 6 cut(s) 93, 99, 164, 220, 340, 645
CviQI GTAC 3 cut(s) 207, 264, 390
DpnI GATC 5 cut(s) 19, 283, 320, 508, 625
DpnII GATC 5 cut(s) 17, 281, 318, 506, 623
Eam1104I CTCTTC 1 cut(s) 662
EarI CTCTTC 1 cut(s) 662
EciI GGCGGA 1 cut(s) 676
Eco130I CCWWGG 1 cut(s) 713
Eco147I AGGCCT 1 cut(s) 718
Eco88I CYCGRG 2 cut(s) 259, 696
EcoT14I CCWWGG 1 cut(s) 713
EcoT22I ATGCAT 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 713
FaeI CATG 6 cut(s) 96, 102, 167, 223, 343, 648
FaqI GGGAC 2 cut(s) 345, 619
FatI CATG 6 cut(s) 92, 98, 163, 219, 339, 644
FauI CCCGC 1 cut(s) 699
FauNDI CATATG 1 cut(s) 142
FblI GTMKAC 1 cut(s) 380
Fnu4HI GCNGC 2 cut(s) 150, 304
FokI GGATG 3 cut(s) 64, 255, 539
Fsp4HI GCNGC 2 cut(s) 150, 304
GluI GCNGC 2 cut(s) 150, 304
HaeIII GGCC 3 cut(s) 76, 367, 718
HapII CCGG 2 cut(s) 14, 170
HgaI GACGC 5 cut(s) 67, 177, 208, 342, 523
Hin1I GRCGYC 1 cut(s) 188
Hin1II CATG 6 cut(s) 96, 102, 167, 223, 343, 648
HinfI GANTC 1 cut(s) 489
HpaII CCGG 2 cut(s) 14, 170
HphI GGTGA 4 cut(s) 183, 424, 465, 680
Hpy166II GTNNAC 4 cut(s) 209, 228, 266, 381
Hpy188I TCNGA 3 cut(s) 274, 323, 506
Hpy188III TCNNGA 6 cut(s) 104, 259, 396, 432, 486, 570
Hpy8I GTNNAC 4 cut(s) 209, 228, 266, 381
Hpy99I CGWCG 3 cut(s) 61, 202, 517
HpyAV CCTTC 1 cut(s) 147
HpyCH4III ACNGT 1 cut(s) 213
HpyCH4IV ACGT 3 cut(s) 278, 565, 599
HpyCH4V TGCA 3 cut(s) 92, 167, 405
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 133
HpySE526I ACGT 3 cut(s) 278, 565, 599
Hsp92I GRCGYC 1 cut(s) 188
Hsp92II CATG 6 cut(s) 96, 102, 167, 223, 343, 648
Kzo9I GATC 5 cut(s) 17, 281, 318, 506, 623
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 4 cut(s) 66, 146, 246, 437
LpnPI CCDG 7 cut(s) 27, 58, 117, 183, 222, 345, 570
MaeII ACGT 3 cut(s) 278, 565, 599
MaeIII GTNAC 2 cut(s) 189, 412
MalI GATC 5 cut(s) 19, 283, 320, 508, 625
MboI GATC 5 cut(s) 17, 281, 318, 506, 623
MboII GAAGA 3 cut(s) 45, 652, 679
MluCI AATT 3 cut(s) 400, 652, 726
MlyI GAGTC 1 cut(s) 498
MmeI TCCRAC 2 cut(s) 27, 614
Mph1103I ATGCAT 1 cut(s) 94
MroXI GAANNNNTTC 1 cut(s) 671
MseI TTAA 4 cut(s) 350, 371, 479, 725
MslI CAYNNNNRTG 2 cut(s) 97, 298
MspI CCGG 2 cut(s) 14, 170
MspR9I CCNGG 1 cut(s) 15
Mva1269I GAATGC 2 cut(s) 173, 714
MvnI CGCG 2 cut(s) 202, 461
MwoI GCNNNNNNNGC 2 cut(s) 82, 133
NciI CCSGG 1 cut(s) 15
NdeI CATATG 1 cut(s) 142
NdeII GATC 5 cut(s) 17, 281, 318, 506, 623
NlaIII CATG 6 cut(s) 96, 102, 167, 223, 343, 648
NlaIV GGNNCC 4 cut(s) 148, 248, 302, 439
NmuCI GTSAC 2 cut(s) 189, 412
NsiI ATGCAT 1 cut(s) 94
NspI RCATGY 1 cut(s) 102
PceI AGGCCT 1 cut(s) 718
PciI ACATGT 1 cut(s) 98
PciSI GCTCTTC 1 cut(s) 662
PcsI WCGNNNNNNNCGW 1 cut(s) 662
PctI GAATGC 2 cut(s) 173, 714
PdmI GAANNNNTTC 1 cut(s) 671
PkrI GCNGC 2 cut(s) 151, 305
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
PscI ACATGT 1 cut(s) 98
PshBI ATTAAT 1 cut(s) 725
PspN4I GGNNCC 4 cut(s) 148, 248, 302, 439
RsaI GTAC 3 cut(s) 208, 265, 391
RsaNI GTAC 3 cut(s) 207, 264, 390
RseI CAYNNNNRTG 2 cut(s) 97, 298
SapI GCTCTTC 1 cut(s) 662
SaqAI TTAA 4 cut(s) 350, 371, 479, 725
SatI GCNGC 2 cut(s) 150, 304
Sau3AI GATC 5 cut(s) 17, 281, 318, 506, 623
SchI GAGTC 1 cut(s) 498
ScrFI CCNGG 1 cut(s) 15
SmiMI CAYNNNNRTG 2 cut(s) 97, 298
SmlI CTYRAG 3 cut(s) 423, 432, 676
SmoI CTYRAG 3 cut(s) 423, 432, 676
Sse9I AATT 3 cut(s) 400, 652, 726
SseBI AGGCCT 1 cut(s) 718
SsiI CCGC 9 cut(s) 9, 150, 234, 298, 304, 445, 461, 661, 692
StuI AGGCCT 1 cut(s) 718
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 713
TaaI ACNGT 1 cut(s) 213
TaiI ACGT 3 cut(s) 281, 568, 602
TaqI TCGA 3 cut(s) 56, 656, 721
TasI AATT 3 cut(s) 400, 652, 726
TatI WGTACW 3 cut(s) 206, 263, 389
TauI GCSGC 2 cut(s) 152, 306
Tru1I TTAA 4 cut(s) 350, 371, 479, 725
Tru9I TTAA 4 cut(s) 350, 371, 479, 725
TscAI CASTG 1 cut(s) 216
TseFI GTSAC 2 cut(s) 189, 412
Tsp45I GTSAC 2 cut(s) 189, 412
TspDTI ATGAA 2 cut(s) 129, 328
TspGWI ACGGA 2 cut(s) 431, 623
TspRI CASTG 1 cut(s) 216
VspI ATTAAT 1 cut(s) 725
XapI RAATTY 1 cut(s) 652
XceI RCATGY 1 cut(s) 102
XmiI GTMKAC 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 671
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.