Rroxscaffold_1G00051580

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000852
Physical Location & Seq
Forward (+)
72231570 .. 72232805
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00051580.1

Sequence Viewer

Length: 1236 bp
ATGCCCATGGTCCTCTTCTACCCGAGAGAACCCGACCACAACTACCTGGACCTCATTTCCATCCACGAACAGCGTTGCAGCAAAATCAAGAAGGACTTATCCGACACCTTAGTCAAGTTCTACCCACTAGCCGGAAGAGTCCACGGAACCCAATACGTCGATTGCAACGACGAAGGAGCCTACTACGTTGAAGCCAAGACCGACTGCAAAATGCCTGACATCATCGACAACCCAAACCCTAAGGACTTCAACAAGTTCCTCCCTTTTGAGCTAGACGCAGCTCATGAGCTTCCCGTGTGCTTCCAGGTCACCTTCTTCGCCTGCGGAGGCATGAGCATCGCCATGGGGATGTCCCACAAGGTCGGGGACACCCTCTCCTACTTCACGTTTCTCAATGGCCTGGCGGCTGAGGCTCGTGGGGAGGGCAGCGATATTCGCCCTCCCGATTTTATCTCAGACAAGTACTTCCCCCAAAAGGACTTATCCGGATTCTTCCAACACTGTAGCGGGATCATAAAGCAAAACATCTCAACAAAACGCTTCGTCTTCGACGCTCCAATGGTTCAGGCCATCCGAGCCAAATGCGTCGACACGTCCAGACGCCCTACTCGCGTTGAAGCCCTATCAGCATTCATATGGAGCCGCTACATAGCATCATGCACCGGCATTCAACAAGATGGTGTCACCACCGACGCGATGTACGCTGTTTCTCATGGTGTGAACCTCCGCACGAGAATGGAACCACCACTTCCGGAGAACACATTCGGACACGTGAGTTACGTGCCCCAAACCGACATCGCTGTGCTGCCCGTACCCGTTGACCCGAAAGACGGGACACTTCATGGAGTGGTTAATCACGTCAGAGAGGCCATCAAACAAGTAGACAAAGAGTGCATAAAGAAACCGCAAGAGGGTGACGGACAATTGAGGTTCTTGAGGAGCCGTACGGAGGAGGTGAAACGCGGAGAGGTGGTGTCGTTTAGGTCCACGAGTCTGTGTAGGTTTCCGATCTACGACGCTGATTTTGGATGGGGAAAGCCTATTTATGTTGGTTCTGCCAGCTTTACGTTCAAGAACTTGGTTTGTTTCTTTGACACTAATGTAGGGGACGGAATTGAGGCGTGGATCAATCTGTTGGAGGAAGACATGAAAAAATTCGAGGCGGATGAGGAGCTTCTCAAGTATGTTTCACCCGCCCCGAGTTCGAAGAATGCCAAGGCCTCGATTAATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

411

Amino Acids

46.23

Weight (kDa)

5.85

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 25 - 392 2.2e-62 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccI GTMKAC 2 cut(s) 588, 882
AccII CGCG 3 cut(s) 612, 695, 963
AccIII TCCGGA 2 cut(s) 485, 751
AciI CCGC 9 cut(s) 324, 404, 507, 643, 727, 905, 963, 1163, 1194
AclWI GGATC 2 cut(s) 518, 1133
AcsI RAATTY 1 cut(s) 1154
AcvI CACGTG 1 cut(s) 772
AcyI GRCGYC 1 cut(s) 601
AfaI GTAC 4 cut(s) 464, 701, 813, 946
AfiI CCNNNNNNNGG 5 cut(s) 131, 475, 830, 911, 949
AflIII ACRYGT 2 cut(s) 591, 769
AgsI TTSAA 5 cut(s) 191, 250, 617, 671, 1072
AjiI CACGTC 2 cut(s) 594, 859
AjnI CCWGG 3 cut(s) 45, 303, 399
AluBI AGCT 5 cut(s) 271, 281, 289, 1062, 1174
AluI AGCT 5 cut(s) 271, 281, 289, 1062, 1174
AlwI GGATC 2 cut(s) 518, 1133
Ama87I CYCGRG 2 cut(s) 22, 1198
Aor13HI TCCGGA 2 cut(s) 485, 751
AoxI GGCC 4 cut(s) 397, 567, 867, 1218
ApeKI GCWGC 4 cut(s) 78, 278, 426, 805
ApoI RAATTY 1 cut(s) 1154
ArsI GACNNNNNNTTYG 2 cut(s) 1007, 1039
AseI ATTAAT 1 cut(s) 1227
Asp700I GAANNNNTTC 2 cut(s) 761, 1154
AspS9I GGNCC 3 cut(s) 10, 49, 984
AsuHPI GGTGA 5 cut(s) 301, 676, 926, 967, 1182
AsuII TTCGAA 1 cut(s) 1205
AvaI CYCGRG 2 cut(s) 22, 1198
AvaII GGWCC 3 cut(s) 10, 49, 984
AxyI CCTNAGG 1 cut(s) 240
BaeGI GKGCMC 1 cut(s) 786
BauI CACGAG 3 cut(s) 414, 730, 988
BbrPI CACGTG 1 cut(s) 772
BbsI GAAGAC 2 cut(s) 538, 1149
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 4 cut(s) 90, 290, 438, 792
BccI CCATC 5 cut(s) 68, 578, 671, 878, 1023
BceAI ACGGC 1 cut(s) 927
BcgI CGANNNNNNTGC 4 cut(s) 319, 353, 564, 598
BciT130I CCWGG 3 cut(s) 47, 305, 401
BfaI CTAG 2 cut(s) 128, 272
BfmI CTRYAG 1 cut(s) 502
BisI GCNGC 6 cut(s) 79, 279, 405, 427, 643, 806
BlsI GCNGC 6 cut(s) 80, 280, 406, 428, 644, 807
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 3 cut(s) 47, 305, 401
Bme18I GGWCC 3 cut(s) 10, 49, 984
BmeT110I CYCGRG 2 cut(s) 22, 1198
BmgBI CACGTC 2 cut(s) 594, 859
BmgT120I GGNCC 3 cut(s) 10, 49, 984
BmiI GGNNCC 5 cut(s) 148, 178, 641, 741, 941
BmrFI CCNGG 3 cut(s) 47, 305, 401
BmsI GCATC 2 cut(s) 345, 662
BpiI GAAGAC 2 cut(s) 538, 1149
Bpu10I CCTNAGC 1 cut(s) 408
Bpu14I TTCGAA 1 cut(s) 1205
BpuEI CTTGAG 2 cut(s) 955, 1163
BsaAI YACGTR 2 cut(s) 772, 781
BsaHI GRCGYC 1 cut(s) 601
BsaJI CCNNGG 4 cut(s) 6, 142, 342, 1215
BsaWI WCCGGW 2 cut(s) 485, 751
BsaXI ACNNNNNCTCC 4 cut(s) 359, 389, 957, 987
Bsc4I CCNNNNNNNGG 5 cut(s) 131, 475, 830, 911, 949
Bse118I RCCGGY 1 cut(s) 662
Bse21I CCTNAGG 1 cut(s) 240
BseAI TCCGGA 2 cut(s) 485, 751
BseBI CCWGG 3 cut(s) 47, 305, 401
BseDI CCNNGG 4 cut(s) 6, 142, 342, 1215
BseGI GGATG 5 cut(s) 60, 354, 570, 1034, 1171
BseLI CCNNNNNNNGG 5 cut(s) 131, 475, 830, 911, 949
BseMII CTCAG 2 cut(s) 399, 468
BseRI GAGGAG 3 cut(s) 952, 965, 1184
BseSI GKGCMC 1 cut(s) 786
BseXI GCAGC 4 cut(s) 90, 290, 438, 792
Bsh1236I CGCG 3 cut(s) 612, 695, 963
BshFI GGCC 4 cut(s) 399, 569, 869, 1220
BsiHKCI CYCGRG 2 cut(s) 22, 1198
BsiSI CCGG 4 cut(s) 132, 486, 663, 752
BsiWI CGTACG 1 cut(s) 944
BslFI GGGAC 4 cut(s) 337, 380, 847, 1121
BslI CCNNNNNNNGG 5 cut(s) 131, 475, 830, 911, 949
BsmFI GGGAC 4 cut(s) 337, 380, 847, 1121
BsmI GAATGC 3 cut(s) 629, 666, 1216
BsnI GGCC 4 cut(s) 399, 569, 869, 1220
BsoBI CYCGRG 2 cut(s) 22, 1198
Bsp119I TTCGAA 1 cut(s) 1205
Bsp1286I GDGCHC 1 cut(s) 786
Bsp13I TCCGGA 2 cut(s) 485, 751
Bsp143I GATC 3 cut(s) 510, 1008, 1125
Bsp19I CCATGG 2 cut(s) 6, 342
BspACI CCGC 9 cut(s) 324, 404, 507, 643, 727, 905, 963, 1163, 1194
BspANI GGCC 4 cut(s) 399, 569, 869, 1220
BspCNI CTCAG 2 cut(s) 400, 467
BspEI TCCGGA 2 cut(s) 485, 751
BspFNI CGCG 3 cut(s) 612, 695, 963
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 5 cut(s) 148, 178, 641, 741, 941
BspPI GGATC 2 cut(s) 518, 1133
BspT104I TTCGAA 1 cut(s) 1205
BsrFI RCCGGY 1 cut(s) 662
BssAI RCCGGY 1 cut(s) 662
BssECI CCNNGG 4 cut(s) 6, 142, 342, 1215
BssMI GATC 3 cut(s) 510, 1008, 1125
BssNI GRCGYC 1 cut(s) 601
BssSI CACGAG 3 cut(s) 414, 730, 988
BssT1I CCWWGG 3 cut(s) 6, 342, 1215
Bst2BI CACGAG 3 cut(s) 414, 730, 988
Bst2UI CCWGG 3 cut(s) 47, 305, 401
Bst4CI ACNGT 1 cut(s) 503
Bst6I CTCTTC 2 cut(s) 20, 130
BstACI GRCGYC 1 cut(s) 601
BstBAI YACGTR 2 cut(s) 772, 781
BstBI TTCGAA 1 cut(s) 1205
BstC8I GCNNGC 2 cut(s) 322, 1060
BstDEI CTNAG 4 cut(s) 109, 240, 408, 454
BstDSI CCRYGG 3 cut(s) 6, 142, 342
BstEII GGTNACC 1 cut(s) 307
BstF5I GGATG 5 cut(s) 60, 354, 570, 1034, 1171
BstFNI CGCG 3 cut(s) 612, 695, 963
BstKTI GATC 3 cut(s) 513, 1011, 1128
BstMBI GATC 3 cut(s) 510, 1008, 1125
BstMWI GCNNNNNNNGC 6 cut(s) 410, 435, 575, 609, 626, 701
BstNI CCWGG 3 cut(s) 47, 305, 401
BstPI GGTNACC 1 cut(s) 307
BstSCI CCNGG 3 cut(s) 45, 303, 399
BstSFI CTRYAG 1 cut(s) 502
BstSLI GKGCMC 1 cut(s) 786
BstUI CGCG 3 cut(s) 612, 695, 963
BstV1I GCAGC 4 cut(s) 90, 290, 438, 792
BstV2I GAAGAC 2 cut(s) 538, 1149
Bsu36I CCTNAGG 1 cut(s) 240
BsuRI GGCC 4 cut(s) 399, 569, 869, 1220
BtgI CCRYGG 3 cut(s) 6, 142, 342
BtgZI GCGATG 3 cut(s) 322, 710, 781
BtrI CACGTC 2 cut(s) 594, 859
BtsCI GGATG 5 cut(s) 60, 354, 570, 1034, 1171
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 2 cut(s) 322, 1060
CciI TCATGA 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 662
Cfr13I GGNCC 3 cut(s) 10, 49, 984
CseI GACGC 6 cut(s) 284, 560, 574, 609, 701, 1025
Csp6I GTAC 4 cut(s) 463, 700, 812, 945
CviAII CATG 8 cut(s) 7, 284, 331, 343, 657, 713, 842, 1147
CviQI GTAC 4 cut(s) 463, 700, 812, 945
DdeI CTNAG 4 cut(s) 109, 240, 408, 454
DpnI GATC 3 cut(s) 512, 1010, 1127
DpnII GATC 3 cut(s) 510, 1008, 1125
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 20, 130
EarI CTCTTC 2 cut(s) 20, 130
EciI GGCGGA 1 cut(s) 1178
Eco130I CCWWGG 3 cut(s) 6, 342, 1215
Eco147I AGGCCT 1 cut(s) 1220
Eco47I GGWCC 3 cut(s) 10, 49, 984
Eco72I CACGTG 1 cut(s) 772
Eco81I CCTNAGG 1 cut(s) 240
Eco88I CYCGRG 2 cut(s) 22, 1198
Eco91I GGTNACC 1 cut(s) 307
EcoO65I GGTNACC 1 cut(s) 307
EcoRII CCWGG 3 cut(s) 45, 303, 399
EcoT14I CCWWGG 3 cut(s) 6, 342, 1215
ErhI CCWWGG 3 cut(s) 6, 342, 1215
FaeI CATG 8 cut(s) 10, 287, 334, 346, 660, 716, 845, 1150
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FaqI GGGAC 4 cut(s) 337, 380, 847, 1121
FatI CATG 8 cut(s) 6, 283, 330, 342, 656, 712, 841, 1146
FauI CCCGC 2 cut(s) 500, 1201
FauNDI CATATG 1 cut(s) 635
FblI GTMKAC 2 cut(s) 588, 882
Fnu4HI GCNGC 6 cut(s) 79, 279, 405, 427, 643, 806
FokI GGATG 5 cut(s) 47, 361, 557, 1041, 1178
Fsp4HI GCNGC 6 cut(s) 79, 279, 405, 427, 643, 806
FspBI CTAG 2 cut(s) 128, 272
GluI GCNGC 6 cut(s) 79, 279, 405, 427, 643, 806
HaeIII GGCC 4 cut(s) 399, 569, 869, 1220
HapII CCGG 4 cut(s) 132, 486, 663, 752
HgaI GACGC 6 cut(s) 284, 560, 574, 609, 701, 1025
Hin1I GRCGYC 1 cut(s) 601
Hin1II CATG 8 cut(s) 10, 287, 334, 346, 660, 716, 845, 1150
HincII GTYRAC 2 cut(s) 589, 820
HindII GTYRAC 2 cut(s) 589, 820
HinfI GANTC 3 cut(s) 138, 489, 991
HpaII CCGG 4 cut(s) 132, 486, 663, 752
HphI GGTGA 5 cut(s) 301, 676, 926, 967, 1182
Hpy166II GTNNAC 6 cut(s) 142, 589, 721, 820, 883, 987
Hpy188I TCNGA 6 cut(s) 103, 457, 575, 767, 863, 1008
Hpy188III TCNNGA 8 cut(s) 88, 284, 443, 486, 597, 752, 934, 1072
Hpy8I GTNNAC 6 cut(s) 142, 589, 721, 820, 883, 987
Hpy99I CGWCG 6 cut(s) 161, 173, 554, 590, 695, 1019
HpyAV CCTTC 3 cut(s) 85, 167, 322
HpyCH4III ACNGT 1 cut(s) 503
HpyCH4IV ACGT 8 cut(s) 156, 186, 386, 593, 771, 780, 858, 1067
HpyCH4V TGCA 5 cut(s) 78, 165, 207, 660, 894
HpyF10VI GCNNNNNNNGC 6 cut(s) 410, 435, 575, 609, 626, 701
HpyF3I CTNAG 4 cut(s) 109, 240, 408, 454
HpySE526I ACGT 8 cut(s) 156, 186, 386, 593, 771, 780, 858, 1067
Hsp92I GRCGYC 1 cut(s) 601
Hsp92II CATG 8 cut(s) 10, 287, 334, 346, 660, 716, 845, 1150
Kpn2I TCCGGA 2 cut(s) 485, 751
Kzo9I GATC 3 cut(s) 510, 1008, 1125
LmnI GCTCC 5 cut(s) 176, 559, 639, 939, 1171
Lsp1109I GCAGC 4 cut(s) 90, 290, 438, 792
LweI GCATC 2 cut(s) 345, 662
MaeI CTAG 2 cut(s) 128, 272
MaeII ACGT 8 cut(s) 156, 186, 386, 593, 771, 780, 858, 1067
MaeIII GTNAC 4 cut(s) 307, 682, 776, 914
MalI GATC 3 cut(s) 512, 1010, 1127
MboI GATC 3 cut(s) 510, 1008, 1125
MboII GAAGA 7 cut(s) 7, 147, 307, 484, 538, 1154, 1219
MfeI CAATTG 1 cut(s) 923
MhlI GDGCHC 1 cut(s) 786
MluCI AATT 4 cut(s) 923, 1113, 1154, 1228
MlyI GAGTC 2 cut(s) 147, 1000
MmeI TCCRAC 3 cut(s) 126, 520, 1116
MroI TCCGGA 2 cut(s) 485, 751
MroXI GAANNNNTTC 2 cut(s) 761, 1154
MseI TTAA 2 cut(s) 852, 1227
MslI CAYNNNNRTG 5 cut(s) 341, 347, 634, 734, 800
MspI CCGG 4 cut(s) 132, 486, 663, 752
MspR9I CCNGG 3 cut(s) 47, 305, 401
MunI CAATTG 1 cut(s) 923
Mva1269I GAATGC 3 cut(s) 629, 666, 1216
MvaI CCWGG 3 cut(s) 47, 305, 401
MvnI CGCG 3 cut(s) 612, 695, 963
MwoI GCNNNNNNNGC 6 cut(s) 410, 435, 575, 609, 626, 701
NcoI CCATGG 2 cut(s) 6, 342
NdeI CATATG 1 cut(s) 635
NdeII GATC 3 cut(s) 510, 1008, 1125
NlaIII CATG 8 cut(s) 10, 287, 334, 346, 660, 716, 845, 1150
NlaIV GGNNCC 5 cut(s) 148, 178, 641, 741, 941
NmuCI GTSAC 3 cut(s) 307, 682, 914
NspV TTCGAA 1 cut(s) 1205
PagI TCATGA 1 cut(s) 283
PceI AGGCCT 1 cut(s) 1220
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 777
PctI GAATGC 3 cut(s) 629, 666, 1216
PdmI GAANNNNTTC 2 cut(s) 761, 1154
PfeI GAWTC 1 cut(s) 489
Pfl23II CGTACG 1 cut(s) 944
PkrI GCNGC 6 cut(s) 80, 280, 406, 428, 644, 807
PleI GAGTC 2 cut(s) 146, 999
PmaCI CACGTG 1 cut(s) 772
PmlI CACGTG 1 cut(s) 772
PpsI GAGTC 2 cut(s) 146, 999
Ppu21I YACGTR 2 cut(s) 772, 781
PshBI ATTAAT 1 cut(s) 1227
Psp6I CCWGG 3 cut(s) 45, 303, 399
PspCI CACGTG 1 cut(s) 772
PspEI GGTNACC 1 cut(s) 307
PspGI CCWGG 3 cut(s) 45, 303, 399
PspLI CGTACG 1 cut(s) 944
PspN4I GGNNCC 5 cut(s) 148, 178, 641, 741, 941
PspPI GGNCC 3 cut(s) 10, 49, 984
RsaI GTAC 4 cut(s) 464, 701, 813, 946
RsaNI GTAC 4 cut(s) 463, 700, 812, 945
RseI CAYNNNNRTG 5 cut(s) 341, 347, 634, 734, 800
SalI GTCGAC 1 cut(s) 587
SaqAI TTAA 2 cut(s) 852, 1227
SatI GCNGC 6 cut(s) 79, 279, 405, 427, 643, 806
Sau3AI GATC 3 cut(s) 510, 1008, 1125
Sau96I GGNCC 3 cut(s) 10, 49, 984
ScaI AGTACT 1 cut(s) 464
SchI GAGTC 2 cut(s) 147, 1000
ScrFI CCNGG 3 cut(s) 47, 305, 401
SduI GDGCHC 1 cut(s) 786
SfaNI GCATC 2 cut(s) 345, 662
SfcI CTRYAG 1 cut(s) 502
SfuI TTCGAA 1 cut(s) 1205
SinI GGWCC 3 cut(s) 10, 49, 984
SmiMI CAYNNNNRTG 5 cut(s) 341, 347, 634, 734, 800
SmlI CTYRAG 2 cut(s) 934, 1178
SmoI CTYRAG 2 cut(s) 934, 1178
Sse9I AATT 4 cut(s) 923, 1113, 1154, 1228
SseBI AGGCCT 1 cut(s) 1220
SsiI CCGC 9 cut(s) 324, 404, 507, 643, 727, 905, 963, 1163, 1194
SspMI CTAG 2 cut(s) 128, 272
StuI AGGCCT 1 cut(s) 1220
StyD4I CCNGG 3 cut(s) 45, 303, 399
StyI CCWWGG 3 cut(s) 6, 342, 1215
TaaI ACNGT 1 cut(s) 503
TaiI ACGT 8 cut(s) 159, 189, 389, 596, 774, 783, 861, 1070
TaqI TCGA 7 cut(s) 159, 225, 549, 588, 1158, 1205, 1223
TaqII GACCGA 1 cut(s) 215
TasI AATT 4 cut(s) 923, 1113, 1154, 1228
TatI WGTACW 1 cut(s) 462
TauI GCSGC 2 cut(s) 407, 645
TfiI GAWTC 1 cut(s) 489
Tru1I TTAA 2 cut(s) 852, 1227
Tru9I TTAA 2 cut(s) 852, 1227
TscAI CASTG 1 cut(s) 506
TseFI GTSAC 3 cut(s) 307, 682, 914
TseI GCWGC 4 cut(s) 78, 278, 426, 805
Tsp45I GTSAC 3 cut(s) 307, 682, 914
TspDTI ATGAA 3 cut(s) 622, 830, 1163
TspGWI ACGGA 4 cut(s) 159, 933, 962, 1125
TspRI CASTG 1 cut(s) 506
VpaK11BI GGWCC 3 cut(s) 10, 49, 984
VspI ATTAAT 1 cut(s) 1227
XapI RAATTY 1 cut(s) 1154
XmiI GTMKAC 2 cut(s) 588, 882
XmnI GAANNNNTTC 2 cut(s) 761, 1154
XspI CTAG 2 cut(s) 128, 272
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.