Rw4G026940

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000852
Physical Location & Seq
Reverse (-)
53228596 .. 53229939
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026940.1

Sequence Viewer

Length: 1344 bp
ATGAAGTTTCAAGTGGAGGTTCTCTCAACAGAGACAATCAAACCCTCATCTCCAACCCCTCACCACCTCCGCCACCACAAGCTCTCCTACCTCGACCAAATCCAGCCCCCAATCTTCATGCCCATGGTCCTCTTCTACCCGAAAGAACCCGACCACAACGACCTGGACCTCATTTCCATCCACGAACAGCGATGCAGCAAAATCAAGAAGGCCTTATCCGACACCTTAGTCAAGTTCTACCCACTAGCCGGAAGAGTCCACGGAACCCAATACGTCGATTGCAACGACGAAGGAGCCTACTACGTCGAAGCCAAGACCGACTGCAAAATCTCTGACATCATCAACAACCCAAACCCCAAGGACTTCAACAAGTTCCTCCCTTTCGAGCTAGACGCAGCTCATGAGCTTCCCGTGTGCTTCCAGGTCACCTTCTTCGCTTGCGGCGGCATGAGTATCGCCATGGGGATGTCCCACAAGGTCGGGGACGCCCTCTCCTACTTCACGTTCCTCAATGGCCTGGCCGCTGAGTCTCGTGGGGAGGGCAACGATATTCACCCTCCCGAGTTTGTCTCAGACAAGTACTTCCCCCAAAAGGACTTGTCCGGATTCTACCAACACCGCAGCGGAATCATAAAGCAAAACATCTCAACAAAACGCTTCGTCTTCGAAGCTCCAATGGTTCAGGCCATCCGAGCCAAATGCATCGACATGTCCAGACGCCCCACTCGTGTTGAAGCCTTATCAGCCTTCATATGGAGCCGCTACATAGCATCATGCACCGGCATTCAACGAGATGGCGTCACCACCAACGCGACGTACACTGTTTCTCATGCTGTGAACCTCCGCACGAGAATGGAGCCACCACTTCCGGAGTACACATTCGGAAACGTGACCCGAACTGCCATTGCGGTGCCGCCCGTACCCGTTGACCCGAAAGACGGGACGCTTCATGGGGTGGTTAATCACGTCAGGGAGGCCATTAAGCAAGTAGACAAAGAGTACATAAAGAAACTACAAGAGGGTGATGAACACTTGAGCTTCTTGAGGAGCCTTTCGGAGGAGGTGAAACGCGGAGAGGTGGTGTCGTTTAGCTTCACGAGTCTGTGTAGGTTTCCGATCTACGACGCTGATTTTGGATGGGGGAAGCCTGTTTATGTTGGTTCTGCCAGCTTTACGTTCAAAAACTTGGTTAGTTTCTTTGACACCAACGTAGGGGACGGAGTTGAGGCGTGGATCAATCTGTTGGAGGAAGACATGGAAACATTCGAGGCGGACGAGGAGCTTCTCAAGTATGTTTCACCCGCCCTGAGTTCAAAGAATGCCAAGGCCTCGATTAATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

447

Amino Acids

50.38

Weight (kDa)

6.03

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 428 1.3e-79 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
AccB1I GGYRCC 1 cut(s) 910
AccI GTMKAC 1 cut(s) 990
AccII CGCG 2 cut(s) 812, 1071
AccIII TCCGGA 2 cut(s) 602, 868
AclWI GGATC 1 cut(s) 1241
AcoI YGGCCR 1 cut(s) 519
AcyI GRCGYC 3 cut(s) 486, 718, 798
AfaI GTAC 5 cut(s) 581, 818, 875, 921, 1001
AfiI CCNNNNNNNGG 6 cut(s) 248, 592, 753, 938, 1057, 1212
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 6 cut(s) 11, 367, 734, 788, 1180, 1314
AjiI CACGTC 1 cut(s) 967
AjnI CCWGG 3 cut(s) 162, 420, 516
AluBI AGCT 9 cut(s) 82, 388, 398, 406, 671, 1038, 1092, 1170, 1282
AluI AGCT 9 cut(s) 82, 388, 398, 406, 671, 1038, 1092, 1170, 1282
Alw26I GTCTC 3 cut(s) 26, 534, 574
AlwI GGATC 1 cut(s) 1241
Ama87I CYCGRG 1 cut(s) 560
Aor13HI TCCGGA 2 cut(s) 602, 868
AoxI GGCC 6 cut(s) 210, 514, 519, 684, 975, 1326
ApeKI GCWGC 3 cut(s) 195, 395, 621
ArsI GACNNNNNNTTYG 2 cut(s) 1115, 1147
AseI ATTAAT 1 cut(s) 1335
AspS9I GGNCC 2 cut(s) 127, 166
AsuHPI GGTGA 7 cut(s) 53, 418, 545, 793, 1034, 1075, 1290
AsuII TTCGAA 1 cut(s) 666
AvaI CYCGRG 1 cut(s) 560
AvaII GGWCC 2 cut(s) 127, 166
BanI GGYRCC 1 cut(s) 910
BauI CACGAG 4 cut(s) 531, 726, 847, 1096
BbsI GAAGAC 2 cut(s) 655, 1257
BbvI GCAGC 3 cut(s) 207, 407, 633
BccI CCATC 4 cut(s) 185, 695, 788, 1131
BcgI CGANNNNNNTGC 4 cut(s) 436, 470, 681, 715
BciT130I CCWGG 3 cut(s) 164, 422, 518
BcoDI GTCTC 3 cut(s) 26, 534, 574
BfaI CTAG 2 cut(s) 245, 389
BisI GCNGC 8 cut(s) 196, 396, 442, 445, 522, 622, 760, 914
BlsI GCNGC 8 cut(s) 197, 397, 443, 446, 523, 623, 761, 915
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 3 cut(s) 164, 422, 518
Bme18I GGWCC 2 cut(s) 127, 166
BmeT110I CYCGRG 1 cut(s) 560
BmgBI CACGTC 1 cut(s) 967
BmgT120I GGNCC 2 cut(s) 127, 166
BmiI GGNNCC 6 cut(s) 265, 295, 758, 858, 912, 1049
BmrFI CCNGG 3 cut(s) 164, 422, 518
BmsI GCATC 3 cut(s) 182, 711, 779
BpiI GAAGAC 2 cut(s) 655, 1257
BplI GAGNNNNNCTC 4 cut(s) 8, 40, 554, 586
Bpu14I TTCGAA 1 cut(s) 666
BpuEI CTTGAG 3 cut(s) 1054, 1063, 1271
BsaHI GRCGYC 3 cut(s) 486, 718, 798
BsaJI CCNNGG 5 cut(s) 123, 259, 357, 459, 1323
BsaWI WCCGGW 2 cut(s) 602, 868
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 476, 506, 1065, 1095
Bsc4I CCNNNNNNNGG 6 cut(s) 248, 592, 753, 938, 1057, 1212
Bse118I RCCGGY 1 cut(s) 779
Bse3DI GCAATG 1 cut(s) 903
BseAI TCCGGA 2 cut(s) 602, 868
BseBI CCWGG 3 cut(s) 164, 422, 518
BseDI CCNNGG 5 cut(s) 123, 259, 357, 459, 1323
BseGI GGATG 4 cut(s) 177, 471, 687, 1142
BseLI CCNNNNNNNGG 6 cut(s) 248, 592, 753, 938, 1057, 1212
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 3 cut(s) 516, 585, 1298
BseRI GAGGAG 3 cut(s) 1060, 1073, 1292
BseXI GCAGC 3 cut(s) 207, 407, 633
Bsh1236I CGCG 2 cut(s) 812, 1071
BshFI GGCC 6 cut(s) 212, 516, 521, 686, 977, 1328
BshNI GGYRCC 1 cut(s) 910
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 4 cut(s) 249, 603, 780, 869
BslFI GGGAC 4 cut(s) 454, 497, 955, 1229
BslI CCNNNNNNNGG 6 cut(s) 248, 592, 753, 938, 1057, 1212
BsmAI GTCTC 3 cut(s) 26, 534, 574
BsmFI GGGAC 4 cut(s) 454, 497, 955, 1229
BsmI GAATGC 2 cut(s) 783, 1324
BsnI GGCC 6 cut(s) 212, 516, 521, 686, 977, 1328
BsoBI CYCGRG 1 cut(s) 560
Bsp119I TTCGAA 1 cut(s) 666
Bsp13I TCCGGA 2 cut(s) 602, 868
Bsp143I GATC 2 cut(s) 1116, 1233
Bsp19I CCATGG 2 cut(s) 123, 459
BspANI GGCC 6 cut(s) 212, 516, 521, 686, 977, 1328
BspCNI CTCAG 3 cut(s) 517, 584, 1299
BspEI TCCGGA 2 cut(s) 602, 868
BspFNI CGCG 2 cut(s) 812, 1071
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 6 cut(s) 265, 295, 758, 858, 912, 1049
BspPI GGATC 1 cut(s) 1241
BspT104I TTCGAA 1 cut(s) 666
BspT107I GGYRCC 1 cut(s) 910
BsrDI GCAATG 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 779
BssAI RCCGGY 1 cut(s) 779
BssECI CCNNGG 5 cut(s) 123, 259, 357, 459, 1323
BssMI GATC 2 cut(s) 1116, 1233
BssNI GRCGYC 3 cut(s) 486, 718, 798
BssSI CACGAG 4 cut(s) 531, 726, 847, 1096
BssT1I CCWWGG 4 cut(s) 123, 357, 459, 1323
Bst2BI CACGAG 4 cut(s) 531, 726, 847, 1096
Bst2UI CCWGG 3 cut(s) 164, 422, 518
Bst4CI ACNGT 1 cut(s) 823
Bst6I CTCTTC 2 cut(s) 137, 247
BstACI GRCGYC 3 cut(s) 486, 718, 798
BstBI TTCGAA 1 cut(s) 666
BstC8I GCNNGC 2 cut(s) 439, 1168
BstDEI CTNAG 4 cut(s) 226, 525, 571, 1307
BstDSI CCRYGG 3 cut(s) 123, 259, 459
BstEII GGTNACC 1 cut(s) 424
BstENI CCTNNNNNAGG 1 cut(s) 1055
BstF5I GGATG 4 cut(s) 177, 471, 687, 1142
BstFNI CGCG 2 cut(s) 812, 1071
BstKTI GATC 2 cut(s) 1119, 1236
BstMAI GTCTC 3 cut(s) 26, 534, 574
BstMBI GATC 2 cut(s) 1116, 1233
BstMWI GCNNNNNNNGC 2 cut(s) 692, 743
BstNI CCWGG 3 cut(s) 164, 422, 518
BstNSI RCATGY 1 cut(s) 712
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 3 cut(s) 162, 420, 516
BstUI CGCG 2 cut(s) 812, 1071
BstV1I GCAGC 3 cut(s) 207, 407, 633
BstV2I GAAGAC 2 cut(s) 655, 1257
BsuRI GGCC 6 cut(s) 212, 516, 521, 686, 977, 1328
BtgI CCRYGG 3 cut(s) 123, 259, 459
BtgZI GCGATG 1 cut(s) 205
BtrI CACGTC 1 cut(s) 967
BtsCI GGATG 4 cut(s) 177, 471, 687, 1142
BtsIMutI CAGTG 1 cut(s) 819
Cac8I GCNNGC 2 cut(s) 439, 1168
CciI TCATGA 1 cut(s) 400
Cfr10I RCCGGY 1 cut(s) 779
Cfr13I GGNCC 2 cut(s) 127, 166
CseI GACGC 6 cut(s) 401, 494, 726, 787, 952, 1133
Csp6I GTAC 5 cut(s) 580, 817, 874, 920, 1000
CspCI CAANNNNNGTGG 2 cut(s) 712, 747
CviQI GTAC 5 cut(s) 580, 817, 874, 920, 1000
DdeI CTNAG 4 cut(s) 226, 525, 571, 1307
DpnI GATC 2 cut(s) 1118, 1235
DpnII GATC 2 cut(s) 1116, 1233
DrdI GACNNNNNNGTC 1 cut(s) 227
DseDI GACNNNNNNGTC 1 cut(s) 227
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 137, 247
EarI CTCTTC 2 cut(s) 137, 247
EciI GGCGGA 2 cut(s) 59, 1286
Eco130I CCWWGG 4 cut(s) 123, 357, 459, 1323
Eco147I AGGCCT 2 cut(s) 212, 1328
Eco47I GGWCC 2 cut(s) 127, 166
Eco88I CYCGRG 1 cut(s) 560
Eco91I GGTNACC 1 cut(s) 424
EcoNI CCTNNNNNAGG 1 cut(s) 1055
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 3 cut(s) 162, 420, 516
EcoT14I CCWWGG 4 cut(s) 123, 357, 459, 1323
EcoT22I ATGCAT 1 cut(s) 704
ErhI CCWWGG 4 cut(s) 123, 357, 459, 1323
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 4 cut(s) 454, 497, 955, 1229
FauI CCCGC 1 cut(s) 1309
FauNDI CATATG 1 cut(s) 752
FblI GTMKAC 1 cut(s) 990
Fnu4HI GCNGC 8 cut(s) 196, 396, 442, 445, 522, 622, 760, 914
FokI GGATG 4 cut(s) 164, 478, 674, 1149
Fsp4HI GCNGC 8 cut(s) 196, 396, 442, 445, 522, 622, 760, 914
FspBI CTAG 2 cut(s) 245, 389
GluI GCNGC 8 cut(s) 196, 396, 442, 445, 522, 622, 760, 914
HaeIII GGCC 6 cut(s) 212, 516, 521, 686, 977, 1328
HapII CCGG 4 cut(s) 249, 603, 780, 869
HgaI GACGC 6 cut(s) 401, 494, 726, 787, 952, 1133
Hin1I GRCGYC 3 cut(s) 486, 718, 798
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HinfI GANTC 5 cut(s) 255, 527, 606, 627, 1099
HpaII CCGG 4 cut(s) 249, 603, 780, 869
HphI GGTGA 7 cut(s) 53, 418, 545, 793, 1034, 1075, 1290
Hpy166II GTNNAC 6 cut(s) 259, 819, 838, 876, 928, 991
Hpy188I TCNGA 7 cut(s) 220, 334, 574, 692, 884, 1057, 1116
Hpy188III TCNNGA 8 cut(s) 205, 401, 560, 603, 714, 869, 1042, 1096
Hpy8I GTNNAC 6 cut(s) 259, 819, 838, 876, 928, 991
Hpy99I CGWCG 5 cut(s) 278, 290, 308, 817, 1127
HpyAV CCTTC 4 cut(s) 202, 284, 439, 757
HpyCH4III ACNGT 1 cut(s) 823
HpyCH4IV ACGT 8 cut(s) 273, 303, 503, 815, 888, 966, 1175, 1209
HpyCH4V TGCA 5 cut(s) 195, 282, 324, 702, 777
HpyF10VI GCNNNNNNNGC 2 cut(s) 692, 743
HpyF3I CTNAG 4 cut(s) 226, 525, 571, 1307
HpySE526I ACGT 8 cut(s) 273, 303, 503, 815, 888, 966, 1175, 1209
Hsp92I GRCGYC 3 cut(s) 486, 718, 798
Kpn2I TCCGGA 2 cut(s) 602, 868
Kzo9I GATC 2 cut(s) 1116, 1233
LmnI GCTCC 6 cut(s) 293, 676, 756, 856, 1047, 1279
Lsp1109I GCAGC 3 cut(s) 207, 407, 633
LweI GCATC 3 cut(s) 182, 711, 779
MaeI CTAG 2 cut(s) 245, 389
MaeII ACGT 8 cut(s) 273, 303, 503, 815, 888, 966, 1175, 1209
MaeIII GTNAC 3 cut(s) 424, 799, 889
MalI GATC 2 cut(s) 1118, 1235
MboI GATC 2 cut(s) 1116, 1233
MboII GAAGA 6 cut(s) 106, 124, 264, 424, 655, 1262
MlyI GAGTC 3 cut(s) 264, 536, 1108
MmeI TCCRAC 3 cut(s) 77, 243, 1224
Mph1103I ATGCAT 1 cut(s) 704
MroI TCCGGA 2 cut(s) 602, 868
MseI TTAA 3 cut(s) 960, 981, 1335
MslI CAYNNNNRTG 5 cut(s) 122, 464, 707, 851, 908
MspA1I CMGCKG 2 cut(s) 524, 624
MspI CCGG 4 cut(s) 249, 603, 780, 869
MspR9I CCNGG 3 cut(s) 164, 422, 518
Mva1269I GAATGC 2 cut(s) 783, 1324
MvaI CCWGG 3 cut(s) 164, 422, 518
MvnI CGCG 2 cut(s) 812, 1071
MwoI GCNNNNNNNGC 2 cut(s) 692, 743
NcoI CCATGG 2 cut(s) 123, 459
NdeI CATATG 1 cut(s) 752
NdeII GATC 2 cut(s) 1116, 1233
NlaIV GGNNCC 6 cut(s) 265, 295, 758, 858, 912, 1049
NmuCI GTSAC 3 cut(s) 424, 799, 889
NsiI ATGCAT 1 cut(s) 704
NspI RCATGY 1 cut(s) 712
NspV TTCGAA 1 cut(s) 666
PagI TCATGA 1 cut(s) 400
PceI AGGCCT 2 cut(s) 212, 1328
PciI ACATGT 1 cut(s) 708
PcsI WCGNNNNNNNCGW 3 cut(s) 282, 724, 1272
PctI GAATGC 2 cut(s) 783, 1324
PfeI GAWTC 2 cut(s) 606, 627
PkrI GCNGC 8 cut(s) 197, 397, 443, 446, 523, 623, 761, 915
PleI GAGTC 3 cut(s) 263, 535, 1107
PpsI GAGTC 3 cut(s) 263, 535, 1107
PscI ACATGT 1 cut(s) 708
PshBI ATTAAT 1 cut(s) 1335
Psp6I CCWGG 3 cut(s) 162, 420, 516
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 3 cut(s) 162, 420, 516
PspN4I GGNNCC 6 cut(s) 265, 295, 758, 858, 912, 1049
PspPI GGNCC 2 cut(s) 127, 166
RsaI GTAC 5 cut(s) 581, 818, 875, 921, 1001
RsaNI GTAC 5 cut(s) 580, 817, 874, 920, 1000
RseI CAYNNNNRTG 5 cut(s) 122, 464, 707, 851, 908
SaqAI TTAA 3 cut(s) 960, 981, 1335
SatI GCNGC 8 cut(s) 196, 396, 442, 445, 522, 622, 760, 914
Sau3AI GATC 2 cut(s) 1116, 1233
Sau96I GGNCC 2 cut(s) 127, 166
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 3 cut(s) 264, 536, 1108
ScrFI CCNGG 3 cut(s) 164, 422, 518
SfaNI GCATC 3 cut(s) 182, 711, 779
SfuI TTCGAA 1 cut(s) 666
SinI GGWCC 2 cut(s) 127, 166
SmiMI CAYNNNNRTG 5 cut(s) 122, 464, 707, 851, 908
SmlI CTYRAG 3 cut(s) 1033, 1042, 1286
SmoI CTYRAG 3 cut(s) 1033, 1042, 1286
SseBI AGGCCT 2 cut(s) 212, 1328
SspMI CTAG 2 cut(s) 245, 389
StuI AGGCCT 2 cut(s) 212, 1328
StyD4I CCNGG 3 cut(s) 162, 420, 516
StyI CCWWGG 4 cut(s) 123, 357, 459, 1323
TaaI ACNGT 1 cut(s) 823
TaiI ACGT 8 cut(s) 276, 306, 506, 818, 891, 969, 1178, 1212
TaqI TCGA 8 cut(s) 93, 276, 306, 384, 666, 705, 1266, 1331
TaqII GACCGA 1 cut(s) 332
TatI WGTACW 3 cut(s) 579, 873, 999
TauI GCSGC 5 cut(s) 444, 447, 524, 762, 916
TfiI GAWTC 2 cut(s) 606, 627
Tru1I TTAA 3 cut(s) 960, 981, 1335
Tru9I TTAA 3 cut(s) 960, 981, 1335
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 3 cut(s) 424, 799, 889
TseI GCWGC 3 cut(s) 195, 395, 621
Tsp45I GTSAC 3 cut(s) 424, 799, 889
TspDTI ATGAA 5 cut(s) 17, 106, 739, 938, 1041
TspGWI ACGGA 2 cut(s) 276, 1233
TspRI CASTG 1 cut(s) 826
VpaK11BI GGWCC 2 cut(s) 127, 166
VspI ATTAAT 1 cut(s) 1335
XagI CCTNNNNNAGG 1 cut(s) 1055
XceI RCATGY 1 cut(s) 712
XmiI GTMKAC 1 cut(s) 990
XspI CTAG 2 cut(s) 245, 389
ZrmI AGTACT 1 cut(s) 581
Zsp2I ATGCAT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.