Rw4G026930

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000852
Physical Location & Seq
Reverse (-)
53219742 .. 53221311
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026930.1

Sequence Viewer

Length: 1293 bp
ATGAAGGTTTTGGTGATTGAGAGGGATTGCATCAAGCCTTCTTCTCCAACCCCAAACGACCTTCGCAAACATGAGCTTTCATTTCTCGATCAAGTCGCCCCTCCCATTTTCATGCCCATGCTCCTATTCTACCCTGCTACTAATTATGGTCACGGTAATGACGAAAACGCTTTGTCTGCTAGCCGGGTTAAGCAGTCCCTATCCGAGACTTTGACGCAGTTCTATCCGCTAGCCGGACGGATCATCGACAACGCCTTTGTCGACTGTAACGATGAAGGTACTCCTTTCGTCGAAGCACGAGTCAACTGCCTACTTTCAGATGTTGTATCCCAACCGGACCCAAACCAACTCAACAAGCTACTCCCATGTGAGTTGGAATGTGTTGTCGACTTTGTTCTTGCAGTCCAAGTCAACATCTTTGACTGTGGAGGCATGGCTATCGGAGTGTGCATTTCGCACAAAATCGCCGACGCGTTGTCACTAGTCATGTTTCTCAACTCTTGGGCTTCCATTTCCCGAGGGGATAATTGTGATTACACAGCTCCCACTTTTGACTTGGCTACGTTCTTCCCGCCGAGATCCATATCCGGGTTCAAACCAGGGACTGGAATCGCAAGAGACAAAATCGTTACTAAAAGATTCGTCTTCAGCGCCTCAACCGTCGCTTCTCTCCGAACAAAATACACGACATCTGCAACTGACAGACGCCCGACTCGTATCGAGGCCCTGTCAGCTTTTGTATGGAGCCGTTTCTTGGCTTCCACGCAAGGAAAGCCCAACTCGGACACAGTTTACAAAGTTCTCCACGCAGTCAACCTACGAACCAGAATGGTCCCGGCGCTTCCGGAGAGTCATTTCGGAAACGTGAGCCGGTTCGCGACTGCGACGACGTCGTTTGAGGAGGGCGAGGAGGAGTGTTATGGGTTTGTGGGACAAATGAGAGATGCCATAAAGACCGTTGACGGGGAGTACATGAGGAAGATTCTAGAAGGCACCGCGCATGTGAATTATTTGAAGCAGCGAGCGGAGGAGTTTCTGAAAGGTGAAGTGGTGTCATTTAGCTTCACCAGTCTCTGTAGGTTTCCATTGTATGAGACTGACTTCGGGTGGGGGAAGCCGATATGGGTCGGCTCTGCTAGCCTCACGTTCAAGAATCTGGTTTGTTTCATGGATTCTGGGTCCGGTGGTGGGATCGAAGCGTGGGTTAATTTGAAGAAAGAAGACATGGCTAAGTTCCAAGAGGATGAGCAGTTACGCGCACTCACTTATCCAGCCAGAGATGCTAAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

430

Amino Acids

47.78

Weight (kDa)

5.81

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 417 5.7e-88 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 893
AccB1I GGYRCC 1 cut(s) 992
AccB7I CCANNNNNTGG 1 cut(s) 605
AccBSI CCGCTC 1 cut(s) 1025
AccI GTMKAC 2 cut(s) 261, 387
AccII CGCG 4 cut(s) 473, 878, 998, 1257
AccIII TCCGGA 1 cut(s) 844
AciI CCGC 4 cut(s) 227, 572, 996, 1025
AclWI GGATC 3 cut(s) 248, 573, 1199
AcuI CTGAAG 1 cut(s) 631
AcyI GRCGYC 2 cut(s) 706, 890
AfaI GTAC 2 cut(s) 280, 971
AfiI CCNNNNNNNGG 6 cut(s) 233, 588, 605, 754, 963, 1188
AflIII ACRYGT 1 cut(s) 471
AgsI TTSAA 4 cut(s) 595, 1015, 1150, 1213
AhdI GACNNNNNGTC 1 cut(s) 475
AhlI ACTAGT 1 cut(s) 481
AjnI CCWGG 1 cut(s) 598
AluBI AGCT 5 cut(s) 76, 358, 542, 734, 1062
AluI AGCT 5 cut(s) 76, 358, 542, 734, 1062
Alw26I GTCTC 4 cut(s) 200, 612, 1076, 1088
AlwI GGATC 3 cut(s) 248, 573, 1199
AlwNI CAGNNNCTG 2 cut(s) 605, 1074
Ama87I CYCGRG 1 cut(s) 516
Aor13HI TCCGGA 1 cut(s) 844
AoxI GGCC 1 cut(s) 723
ApeKI GCWGC 1 cut(s) 1018
ArsI GACNNNNNNTTYG 4 cut(s) 239, 271, 285, 317
AspLEI GCGC 4 cut(s) 653, 841, 1000, 1259
AspS9I GGNCC 4 cut(s) 337, 724, 832, 1179
AsuC2I CCSGG 3 cut(s) 185, 589, 836
AsuHPI GGTGA 3 cut(s) 25, 1055, 1057
AsuNHI GCTAGC 3 cut(s) 179, 229, 1136
AvaI CYCGRG 1 cut(s) 516
AvaII GGWCC 3 cut(s) 337, 832, 1179
BanI GGYRCC 1 cut(s) 992
BauI CACGAG 1 cut(s) 297
BbsI GAAGAC 2 cut(s) 637, 1227
BbvI GCAGC 1 cut(s) 1030
BceAI ACGGC 1 cut(s) 732
BcgI CGANNNNNNTGC 4 cut(s) 288, 322, 421, 455
BciT130I CCWGG 1 cut(s) 600
BciVI GTATCC 1 cut(s) 337
BcnI CCSGG 3 cut(s) 185, 589, 836
BcoDI GTCTC 4 cut(s) 200, 612, 1076, 1088
BcuI ACTAGT 1 cut(s) 481
BfaI CTAG 5 cut(s) 180, 230, 482, 986, 1137
BfmI CTRYAG 1 cut(s) 1075
BfoI RGCGCY 2 cut(s) 654, 842
BfuI GTATCC 1 cut(s) 337
BisI GCNGC 1 cut(s) 1019
BlsI GCNGC 1 cut(s) 1020
Bme1390I CCNGG 4 cut(s) 185, 589, 600, 836
Bme18I GGWCC 3 cut(s) 337, 832, 1179
BmeRI GACNNNNNGTC 1 cut(s) 475
BmeT110I CYCGRG 1 cut(s) 516
BmgT120I GGNCC 4 cut(s) 337, 724, 832, 1179
BmiI GGNNCC 5 cut(s) 339, 746, 834, 994, 1180
BmrFI CCNGG 4 cut(s) 185, 589, 600, 836
BmsI GCATC 3 cut(s) 39, 934, 1270
BmtI GCTAGC 3 cut(s) 183, 233, 1140
BpiI GAAGAC 2 cut(s) 637, 1227
BpuMI CCSGG 3 cut(s) 185, 589, 836
BsaBI GATNNNNATC 1 cut(s) 583
BsaHI GRCGYC 2 cut(s) 706, 890
BsaJI CCNNGG 2 cut(s) 517, 599
BsaWI WCCGGW 3 cut(s) 334, 844, 1181
Bsc4I CCNNNNNNNGG 6 cut(s) 233, 588, 605, 754, 963, 1188
Bse118I RCCGGY 1 cut(s) 870
Bse1I ACTGG 2 cut(s) 610, 1068
Bse8I GATNNNNATC 1 cut(s) 583
BseAI TCCGGA 1 cut(s) 844
BseBI CCWGG 1 cut(s) 600
BseDI CCNNGG 2 cut(s) 517, 599
BseGI GGATG 1 cut(s) 1249
BseJI GATNNNNATC 1 cut(s) 583
BseLI CCNNNNNNNGG 6 cut(s) 233, 588, 605, 754, 963, 1188
BseNI ACTGG 2 cut(s) 610, 1068
BseRI GAGGAG 4 cut(s) 914, 923, 926, 1043
BseXI GCAGC 1 cut(s) 1030
Bsh1236I CGCG 4 cut(s) 473, 878, 998, 1257
BshFI GGCC 1 cut(s) 725
BshNI GGYRCC 1 cut(s) 992
BsiHKCI CYCGRG 1 cut(s) 516
BsiSI CCGG 8 cut(s) 184, 234, 335, 588, 836, 845, 871, 1182
BslFI GGGAC 4 cut(s) 181, 616, 818, 945
BslI CCNNNNNNNGG 6 cut(s) 233, 588, 605, 754, 963, 1188
BsmAI GTCTC 4 cut(s) 200, 612, 1076, 1088
BsmFI GGGAC 4 cut(s) 181, 616, 818, 945
BsnI GGCC 1 cut(s) 725
BsoBI CYCGRG 1 cut(s) 516
Bsp13I TCCGGA 1 cut(s) 844
Bsp143I GATC 4 cut(s) 88, 240, 578, 1191
Bsp68I TCGCGA 1 cut(s) 878
BspACI CCGC 4 cut(s) 227, 572, 996, 1025
BspANI GGCC 1 cut(s) 725
BspEI TCCGGA 1 cut(s) 844
BspFNI CGCG 4 cut(s) 473, 878, 998, 1257
BspLI GGNNCC 5 cut(s) 339, 746, 834, 994, 1180
BspOI GCTAGC 3 cut(s) 183, 233, 1140
BspPI GGATC 3 cut(s) 248, 573, 1199
BspT107I GGYRCC 1 cut(s) 992
BsrBI CCGCTC 1 cut(s) 1025
BsrFI RCCGGY 1 cut(s) 870
BsrI ACTGG 2 cut(s) 610, 1068
BssAI RCCGGY 1 cut(s) 870
BssECI CCNNGG 2 cut(s) 517, 599
BssMI GATC 4 cut(s) 88, 240, 578, 1191
BssNI GRCGYC 2 cut(s) 706, 890
BssSI CACGAG 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 6 cut(s) 155, 266, 425, 661, 790, 958
BstACI GRCGYC 2 cut(s) 706, 890
BstC8I GCNNGC 4 cut(s) 181, 231, 1023, 1138
BstDEI CTNAG 1 cut(s) 1230
BstF5I GGATG 1 cut(s) 1249
BstFNI CGCG 4 cut(s) 473, 878, 998, 1257
BstH2I RGCGCY 2 cut(s) 654, 842
BstHHI GCGC 4 cut(s) 653, 841, 1000, 1259
BstKTI GATC 4 cut(s) 91, 243, 581, 1194
BstMAI GTCTC 4 cut(s) 200, 612, 1076, 1088
BstMBI GATC 4 cut(s) 88, 240, 578, 1191
BstMWI GCNNNNNNNGC 5 cut(s) 176, 731, 772, 1137, 1280
BstNI CCWGG 1 cut(s) 600
BstNSI RCATGY 1 cut(s) 1004
BstSCI CCNGG 4 cut(s) 183, 587, 598, 834
BstSFI CTRYAG 1 cut(s) 1075
BstUI CGCG 4 cut(s) 473, 878, 998, 1257
BstV1I GCAGC 1 cut(s) 1030
BstV2I GAAGAC 2 cut(s) 637, 1227
BstX2I RGATCY 1 cut(s) 578
BstYI RGATCY 1 cut(s) 578
BsuI GTATCC 1 cut(s) 337
BsuRI GGCC 1 cut(s) 725
BtsCI GGATG 1 cut(s) 1249
BtuMI TCGCGA 1 cut(s) 878
Cac8I GCNNGC 4 cut(s) 181, 231, 1023, 1138
CaiI CAGNNNCTG 2 cut(s) 605, 1074
CfoI GCGC 4 cut(s) 653, 841, 1000, 1259
Cfr10I RCCGGY 1 cut(s) 870
Cfr13I GGNCC 4 cut(s) 337, 724, 832, 1179
CseI GACGC 3 cut(s) 223, 479, 714
Csp6I GTAC 2 cut(s) 279, 970
CviQI GTAC 2 cut(s) 279, 970
DdeI CTNAG 1 cut(s) 1230
DpnI GATC 4 cut(s) 90, 242, 580, 1193
DpnII GATC 4 cut(s) 88, 240, 578, 1191
DriI GACNNNNNGTC 1 cut(s) 475
Eam1105I GACNNNNNGTC 1 cut(s) 475
Eco47I GGWCC 3 cut(s) 337, 832, 1179
Eco57I CTGAAG 1 cut(s) 631
Eco88I CYCGRG 1 cut(s) 516
EcoO109I RGGNCCY 1 cut(s) 724
EcoRII CCWGG 1 cut(s) 598
FaqI GGGAC 4 cut(s) 181, 616, 818, 945
FauI CCCGC 1 cut(s) 579
FblI GTMKAC 2 cut(s) 261, 387
Fnu4HI GCNGC 1 cut(s) 1019
FokI GGATG 1 cut(s) 1256
Fsp4HI GCNGC 1 cut(s) 1019
FspBI CTAG 5 cut(s) 180, 230, 482, 986, 1137
GlaI GCGC 4 cut(s) 652, 840, 999, 1258
GluI GCNGC 1 cut(s) 1019
HaeII RGCGCY 2 cut(s) 654, 842
HaeIII GGCC 1 cut(s) 725
HapII CCGG 8 cut(s) 184, 234, 335, 588, 836, 845, 871, 1182
HgaI GACGC 3 cut(s) 223, 479, 714
HhaI GCGC 4 cut(s) 653, 841, 1000, 1259
Hin1I GRCGYC 2 cut(s) 706, 890
Hin6I GCGC 4 cut(s) 651, 839, 998, 1257
HinP1I GCGC 4 cut(s) 651, 839, 998, 1257
HincII GTYRAC 6 cut(s) 262, 304, 388, 412, 814, 961
HindII GTYRAC 6 cut(s) 262, 304, 388, 412, 814, 961
HinfI GANTC 8 cut(s) 300, 609, 639, 712, 850, 982, 1153, 1172
HpaII CCGG 8 cut(s) 184, 234, 335, 588, 836, 845, 871, 1182
HphI GGTGA 3 cut(s) 25, 1055, 1057
Hpy166II GTNNAC 7 cut(s) 262, 304, 388, 412, 793, 814, 961
Hpy188I TCNGA 7 cut(s) 205, 319, 443, 674, 784, 860, 1038
Hpy188III TCNNGA 6 cut(s) 86, 516, 845, 877, 986, 1150
Hpy8I GTNNAC 7 cut(s) 262, 304, 388, 412, 793, 814, 961
Hpy99I CGWCG 6 cut(s) 293, 473, 665, 889, 892, 895
HpyAV CCTTC 4 cut(s) 48, 71, 269, 983
HpyCH4III ACNGT 6 cut(s) 155, 266, 425, 661, 790, 958
HpyCH4IV ACGT 4 cut(s) 563, 864, 890, 1145
HpyCH4V TGCA 4 cut(s) 30, 401, 450, 695
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 731, 772, 1137, 1280
HpyF3I CTNAG 1 cut(s) 1230
HpySE526I ACGT 4 cut(s) 563, 864, 890, 1145
Hsp92I GRCGYC 2 cut(s) 706, 890
HspAI GCGC 4 cut(s) 651, 839, 998, 1257
Kpn2I TCCGGA 1 cut(s) 844
Kzo9I GATC 4 cut(s) 88, 240, 578, 1191
LmnI GCTCC 3 cut(s) 126, 547, 744
Lsp1109I GCAGC 1 cut(s) 1030
LweI GCATC 3 cut(s) 39, 934, 1270
MaeI CTAG 5 cut(s) 180, 230, 482, 986, 1137
MaeII ACGT 4 cut(s) 563, 864, 890, 1145
MaeIII GTNAC 5 cut(s) 149, 266, 477, 628, 1251
MalI GATC 4 cut(s) 90, 242, 580, 1193
MbiI CCGCTC 1 cut(s) 1025
MboI GATC 4 cut(s) 88, 240, 578, 1191
MboII GAAGA 6 cut(s) 33, 559, 637, 991, 1225, 1232
MflI RGATCY 1 cut(s) 578
MluCI AATT 4 cut(s) 142, 526, 1006, 1207
MluI ACGCGT 1 cut(s) 471
MlyI GAGTC 3 cut(s) 309, 706, 859
MmeI TCCRAC 2 cut(s) 71, 354
MroI TCCGGA 1 cut(s) 844
MseI TTAA 2 cut(s) 189, 1206
MslI CAYNNNNRTG 3 cut(s) 110, 116, 156
MspI CCGG 8 cut(s) 184, 234, 335, 588, 836, 845, 871, 1182
MspR9I CCNGG 4 cut(s) 185, 589, 600, 836
MvaI CCWGG 1 cut(s) 600
MvnI CGCG 4 cut(s) 473, 878, 998, 1257
MwoI GCNNNNNNNGC 5 cut(s) 176, 731, 772, 1137, 1280
NciI CCSGG 3 cut(s) 185, 589, 836
NdeII GATC 4 cut(s) 88, 240, 578, 1191
NheI GCTAGC 3 cut(s) 179, 229, 1136
NlaIV GGNNCC 5 cut(s) 339, 746, 834, 994, 1180
NmeAIII GCCGAG 1 cut(s) 600
NmuCI GTSAC 2 cut(s) 149, 477
NruI TCGCGA 1 cut(s) 878
NspI RCATGY 1 cut(s) 1004
PcsI WCGNNNNNNNCGW 4 cut(s) 159, 258, 267, 712
PfeI GAWTC 5 cut(s) 609, 639, 982, 1153, 1172
PflFI GACNNNGTC 1 cut(s) 889
PflMI CCANNNNNTGG 1 cut(s) 605
PkrI GCNGC 1 cut(s) 1020
PleI GAGTC 3 cut(s) 308, 706, 858
PpsI GAGTC 3 cut(s) 308, 706, 858
Psp6I CCWGG 1 cut(s) 598
PspGI CCWGG 1 cut(s) 598
PspN4I GGNNCC 5 cut(s) 339, 746, 834, 994, 1180
PspPI GGNCC 4 cut(s) 337, 724, 832, 1179
PstNI CAGNNNCTG 2 cut(s) 605, 1074
PsuI RGATCY 1 cut(s) 578
PsyI GACNNNGTC 1 cut(s) 889
RruI TCGCGA 1 cut(s) 878
RsaI GTAC 2 cut(s) 280, 971
RsaNI GTAC 2 cut(s) 279, 970
RseI CAYNNNNRTG 3 cut(s) 110, 116, 156
SalI GTCGAC 2 cut(s) 260, 386
SaqAI TTAA 2 cut(s) 189, 1206
SatI GCNGC 1 cut(s) 1019
Sau3AI GATC 4 cut(s) 88, 240, 578, 1191
Sau96I GGNCC 4 cut(s) 337, 724, 832, 1179
SchI GAGTC 3 cut(s) 309, 706, 859
ScrFI CCNGG 4 cut(s) 185, 589, 600, 836
SfaNI GCATC 3 cut(s) 39, 934, 1270
SfcI CTRYAG 1 cut(s) 1075
SinI GGWCC 3 cut(s) 337, 832, 1179
SmiMI CAYNNNNRTG 3 cut(s) 110, 116, 156
SpeI ACTAGT 1 cut(s) 481
Sse9I AATT 4 cut(s) 142, 526, 1006, 1207
SsiI CCGC 4 cut(s) 227, 572, 996, 1025
SspMI CTAG 5 cut(s) 180, 230, 482, 986, 1137
StyD4I CCNGG 4 cut(s) 183, 587, 598, 834
TaaI ACNGT 6 cut(s) 155, 266, 425, 661, 790, 958
TaiI ACGT 4 cut(s) 566, 867, 893, 1148
TaqI TCGA 7 cut(s) 87, 246, 261, 291, 387, 720, 1194
TasI AATT 4 cut(s) 142, 526, 1006, 1207
TatI WGTACW 1 cut(s) 969
TfiI GAWTC 5 cut(s) 609, 639, 982, 1153, 1172
Tru1I TTAA 2 cut(s) 189, 1206
Tru9I TTAA 2 cut(s) 189, 1206
TseFI GTSAC 2 cut(s) 149, 477
TseI GCWGC 1 cut(s) 1018
Tsp45I GTSAC 2 cut(s) 149, 477
TspDTI ATGAA 5 cut(s) 17, 69, 100, 288, 1156
TspGWI ACGGA 1 cut(s) 253
Tth111I GACNNNGTC 1 cut(s) 889
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 3 cut(s) 337, 832, 1179
XbaI TCTAGA 1 cut(s) 985
XceI RCATGY 1 cut(s) 1004
XcmI CCANNNNNNNNNTGG 1 cut(s) 553
XmiI GTMKAC 2 cut(s) 261, 387
XspI CTAG 5 cut(s) 180, 230, 482, 986, 1137
ZraI GACGTC 1 cut(s) 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.