Rmu_co8339999.1_g000001

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8339999.1
OG0000852
Physical Location & Seq
Forward (+)
1 .. 1004
1004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8339999.1_g000001.1.cds

Sequence Viewer

Length: 1004 bp
tacccactagccggaagagtccacggaacccaatacgtcgattgcaacgacgaaggagcctactacgtcgaagccaagaccgactgcaaaatctctgacatcatcaacaacccaaaccccaaggacttcaacaagttcctccctttcgagctagacgcagctcatgagcttcccgtgtgcttccaggtcaccttcttcgcttgcggcggcatgagtatcgccatggggatgtcccacaaggtcggggacgccctctcctacttcacgttcctcaatggcctggccgctgagtctcgtggggagggcaacgatattcaccctcccgagtttgtctcagacaagtacttcccccaaaaggacttgtccgggttctaccaacaccgcagcggaatcataaagcaaaacatctcaacaaaacgcttcgtcttcgaagctccaatggttcaggccatccgagccaaatgcatcgacatgtccagacgccccactcgtgttgaagccttatcagccttcatatggagccgctacatagcatcatgcaccggcattcaacgagatggcgtcaccaccaacgcgacgtacactgtttctcatgctgtgaacctccgcacgagaatggagccaccacttccggagtacacattcggaaacgtgacccgaactgccattgcggtgccgcccgtacccgttgacccgaaagacgggacgcttcatggggtggttaatcacgtcagggaggccattaagcaagtagacaaagagtacataaagaaactacaagagggtgatgaacacttgagcttcttgaggagcctttcggaggaggtgaaacgcggagaggtggtgtcgtttagcttcacgagtctgtgtaggtttccgatctacgacgctgattttggatgggggaagcctgtttatgttggttctgccagctttacgttcaaaaacttggttagtttctttgacaccaacgtaggggacggagttgaggcgtggatcaatct
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

335

Amino Acids

37.46

Weight (kDa)

6.17

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 673
AccI GTMKAC 1 cut(s) 753
AccII CGCG 2 cut(s) 575, 834
AccIII TCCGGA 1 cut(s) 631
AcoI YGGCCR 1 cut(s) 282
AcyI GRCGYC 3 cut(s) 249, 481, 561
AfaI GTAC 5 cut(s) 344, 581, 638, 684, 764
AfiI CCNNNNNNNGG 6 cut(s) 11, 355, 516, 701, 820, 975
AflIII ACRYGT 1 cut(s) 471
AgsI TTSAA 4 cut(s) 130, 497, 551, 943
AjiI CACGTC 1 cut(s) 730
AjnI CCWGG 2 cut(s) 183, 279
AluBI AGCT 7 cut(s) 151, 161, 169, 434, 801, 855, 933
AluI AGCT 7 cut(s) 151, 161, 169, 434, 801, 855, 933
Alw26I GTCTC 2 cut(s) 297, 337
Ama87I CYCGRG 1 cut(s) 323
Aor13HI TCCGGA 1 cut(s) 631
AoxI GGCC 4 cut(s) 277, 282, 447, 738
ApeKI GCWGC 2 cut(s) 158, 384
ArsI GACNNNNNNTTYG 2 cut(s) 878, 910
AsuC2I CCSGG 1 cut(s) 367
AsuHPI GGTGA 5 cut(s) 181, 308, 556, 797, 838
AsuII TTCGAA 1 cut(s) 429
AvaI CYCGRG 1 cut(s) 323
BanI GGYRCC 1 cut(s) 673
BauI CACGAG 4 cut(s) 294, 489, 610, 859
BbsI GAAGAC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 170, 396
BccI CCATC 3 cut(s) 458, 551, 894
BcgI CGANNNNNNTGC 4 cut(s) 199, 233, 444, 478
BciT130I CCWGG 2 cut(s) 185, 281
BcnI CCSGG 1 cut(s) 367
BcoDI GTCTC 2 cut(s) 297, 337
BfaI CTAG 2 cut(s) 8, 152
BisI GCNGC 7 cut(s) 159, 205, 208, 285, 385, 523, 677
BlsI GCNGC 7 cut(s) 160, 206, 209, 286, 386, 524, 678
BmcAI AGTACT 1 cut(s) 344
Bme1390I CCNGG 3 cut(s) 185, 281, 367
BmeT110I CYCGRG 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 730
BmiI GGNNCC 6 cut(s) 28, 58, 521, 621, 675, 812
BmrFI CCNGG 3 cut(s) 185, 281, 367
BmsI GCATC 2 cut(s) 474, 542
BpiI GAAGAC 1 cut(s) 418
BplI GAGNNNNNCTC 2 cut(s) 317, 349
Bpu14I TTCGAA 1 cut(s) 429
BpuEI CTTGAG 2 cut(s) 817, 826
BpuMI CCSGG 1 cut(s) 367
BsaHI GRCGYC 3 cut(s) 249, 481, 561
BsaJI CCNNGG 3 cut(s) 22, 120, 222
BsaWI WCCGGW 1 cut(s) 631
BsaXI ACNNNNNCTCC 4 cut(s) 239, 269, 828, 858
Bsc4I CCNNNNNNNGG 6 cut(s) 11, 355, 516, 701, 820, 975
Bse118I RCCGGY 1 cut(s) 542
Bse3DI GCAATG 1 cut(s) 666
BseAI TCCGGA 1 cut(s) 631
BseBI CCWGG 2 cut(s) 185, 281
BseDI CCNNGG 3 cut(s) 22, 120, 222
BseGI GGATG 3 cut(s) 234, 450, 905
BseLI CCNNNNNNNGG 6 cut(s) 11, 355, 516, 701, 820, 975
BseMI GCAATG 1 cut(s) 666
BseMII CTCAG 2 cut(s) 279, 348
BseRI GAGGAG 2 cut(s) 823, 836
BseXI GCAGC 2 cut(s) 170, 396
Bsh1236I CGCG 2 cut(s) 575, 834
BshFI GGCC 4 cut(s) 279, 284, 449, 740
BshNI GGYRCC 1 cut(s) 673
BsiHKCI CYCGRG 1 cut(s) 323
BsiSI CCGG 4 cut(s) 12, 366, 543, 632
BslFI GGGAC 4 cut(s) 217, 260, 718, 992
BslI CCNNNNNNNGG 6 cut(s) 11, 355, 516, 701, 820, 975
BsmAI GTCTC 2 cut(s) 297, 337
BsmFI GGGAC 4 cut(s) 217, 260, 718, 992
BsmI GAATGC 1 cut(s) 546
BsnI GGCC 4 cut(s) 279, 284, 449, 740
BsoBI CYCGRG 1 cut(s) 323
Bsp119I TTCGAA 1 cut(s) 429
Bsp13I TCCGGA 1 cut(s) 631
Bsp143I GATC 2 cut(s) 879, 996
Bsp19I CCATGG 1 cut(s) 222
BspANI GGCC 4 cut(s) 279, 284, 449, 740
BspCNI CTCAG 2 cut(s) 280, 347
BspEI TCCGGA 1 cut(s) 631
BspFNI CGCG 2 cut(s) 575, 834
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 6 cut(s) 28, 58, 521, 621, 675, 812
BspT104I TTCGAA 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 673
BsrDI GCAATG 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 542
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 3 cut(s) 22, 120, 222
BssMI GATC 2 cut(s) 879, 996
BssNI GRCGYC 3 cut(s) 249, 481, 561
BssSI CACGAG 4 cut(s) 294, 489, 610, 859
BssT1I CCWWGG 2 cut(s) 120, 222
Bst2BI CACGAG 4 cut(s) 294, 489, 610, 859
Bst2UI CCWGG 2 cut(s) 185, 281
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 1 cut(s) 10
BstACI GRCGYC 3 cut(s) 249, 481, 561
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 2 cut(s) 202, 931
BstDEI CTNAG 2 cut(s) 288, 334
BstDSI CCRYGG 2 cut(s) 22, 222
BstEII GGTNACC 1 cut(s) 187
BstENI CCTNNNNNAGG 1 cut(s) 818
BstF5I GGATG 3 cut(s) 234, 450, 905
BstFNI CGCG 2 cut(s) 575, 834
BstKTI GATC 2 cut(s) 882, 999
BstMAI GTCTC 2 cut(s) 297, 337
BstMBI GATC 2 cut(s) 879, 996
BstMWI GCNNNNNNNGC 2 cut(s) 455, 506
BstNI CCWGG 2 cut(s) 185, 281
BstNSI RCATGY 1 cut(s) 475
BstPI GGTNACC 1 cut(s) 187
BstSCI CCNGG 3 cut(s) 183, 279, 365
BstUI CGCG 2 cut(s) 575, 834
BstV1I GCAGC 2 cut(s) 170, 396
BstV2I GAAGAC 1 cut(s) 418
BsuRI GGCC 4 cut(s) 279, 284, 449, 740
BtgI CCRYGG 2 cut(s) 22, 222
BtrI CACGTC 1 cut(s) 730
BtsCI GGATG 3 cut(s) 234, 450, 905
BtsIMutI CAGTG 1 cut(s) 582
Cac8I GCNNGC 2 cut(s) 202, 931
CciI TCATGA 1 cut(s) 163
Cfr10I RCCGGY 1 cut(s) 542
CseI GACGC 6 cut(s) 164, 257, 489, 550, 715, 896
Csp6I GTAC 5 cut(s) 343, 580, 637, 683, 763
CspCI CAANNNNNGTGG 2 cut(s) 475, 510
CviAII CATG 7 cut(s) 164, 211, 223, 472, 537, 593, 713
CviQI GTAC 5 cut(s) 343, 580, 637, 683, 763
DdeI CTNAG 2 cut(s) 288, 334
DpnI GATC 2 cut(s) 881, 998
DpnII GATC 2 cut(s) 879, 996
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 1 cut(s) 10
EarI CTCTTC 1 cut(s) 10
Eco130I CCWWGG 2 cut(s) 120, 222
Eco88I CYCGRG 1 cut(s) 323
Eco91I GGTNACC 1 cut(s) 187
EcoNI CCTNNNNNAGG 1 cut(s) 818
EcoO65I GGTNACC 1 cut(s) 187
EcoRII CCWGG 2 cut(s) 183, 279
EcoT14I CCWWGG 2 cut(s) 120, 222
EcoT22I ATGCAT 1 cut(s) 467
ErhI CCWWGG 2 cut(s) 120, 222
FaeI CATG 7 cut(s) 167, 214, 226, 475, 540, 596, 716
FaqI GGGAC 4 cut(s) 217, 260, 718, 992
FatI CATG 7 cut(s) 163, 210, 222, 471, 536, 592, 712
FauNDI CATATG 1 cut(s) 515
FblI GTMKAC 1 cut(s) 753
Fnu4HI GCNGC 7 cut(s) 159, 205, 208, 285, 385, 523, 677
FokI GGATG 3 cut(s) 241, 437, 912
Fsp4HI GCNGC 7 cut(s) 159, 205, 208, 285, 385, 523, 677
FspBI CTAG 2 cut(s) 8, 152
GluI GCNGC 7 cut(s) 159, 205, 208, 285, 385, 523, 677
HaeIII GGCC 4 cut(s) 279, 284, 449, 740
HapII CCGG 4 cut(s) 12, 366, 543, 632
HgaI GACGC 6 cut(s) 164, 257, 489, 550, 715, 896
Hin1I GRCGYC 3 cut(s) 249, 481, 561
Hin1II CATG 7 cut(s) 167, 214, 226, 475, 540, 596, 716
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HinfI GANTC 4 cut(s) 18, 290, 390, 862
HpaII CCGG 4 cut(s) 12, 366, 543, 632
HphI GGTGA 5 cut(s) 181, 308, 556, 797, 838
Hpy166II GTNNAC 6 cut(s) 22, 582, 601, 639, 691, 754
Hpy188I TCNGA 6 cut(s) 97, 337, 455, 647, 820, 879
Hpy188III TCNNGA 6 cut(s) 164, 323, 477, 632, 805, 859
Hpy8I GTNNAC 6 cut(s) 22, 582, 601, 639, 691, 754
Hpy99I CGWCG 5 cut(s) 41, 53, 71, 580, 890
HpyAV CCTTC 3 cut(s) 47, 202, 520
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 8 cut(s) 36, 66, 266, 578, 651, 729, 938, 972
HpyCH4V TGCA 4 cut(s) 45, 87, 465, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 506
HpyF3I CTNAG 2 cut(s) 288, 334
HpySE526I ACGT 8 cut(s) 36, 66, 266, 578, 651, 729, 938, 972
Hsp92I GRCGYC 3 cut(s) 249, 481, 561
Hsp92II CATG 7 cut(s) 167, 214, 226, 475, 540, 596, 716
Kpn2I TCCGGA 1 cut(s) 631
Kzo9I GATC 2 cut(s) 879, 996
LmnI GCTCC 5 cut(s) 56, 439, 519, 619, 810
Lsp1109I GCAGC 2 cut(s) 170, 396
LweI GCATC 2 cut(s) 474, 542
MaeI CTAG 2 cut(s) 8, 152
MaeII ACGT 8 cut(s) 36, 66, 266, 578, 651, 729, 938, 972
MaeIII GTNAC 3 cut(s) 187, 562, 652
MalI GATC 2 cut(s) 881, 998
MboI GATC 2 cut(s) 879, 996
MboII GAAGA 3 cut(s) 27, 187, 418
MlyI GAGTC 3 cut(s) 27, 299, 871
Mph1103I ATGCAT 1 cut(s) 467
MroI TCCGGA 1 cut(s) 631
MseI TTAA 2 cut(s) 723, 744
MslI CAYNNNNRTG 4 cut(s) 227, 470, 614, 671
MspA1I CMGCKG 2 cut(s) 287, 387
MspI CCGG 4 cut(s) 12, 366, 543, 632
MspR9I CCNGG 3 cut(s) 185, 281, 367
Mva1269I GAATGC 1 cut(s) 546
MvaI CCWGG 2 cut(s) 185, 281
MvnI CGCG 2 cut(s) 575, 834
MwoI GCNNNNNNNGC 2 cut(s) 455, 506
NciI CCSGG 1 cut(s) 367
NcoI CCATGG 1 cut(s) 222
NdeI CATATG 1 cut(s) 515
NdeII GATC 2 cut(s) 879, 996
NlaIII CATG 7 cut(s) 167, 214, 226, 475, 540, 596, 716
NlaIV GGNNCC 6 cut(s) 28, 58, 521, 621, 675, 812
NmuCI GTSAC 3 cut(s) 187, 562, 652
NsiI ATGCAT 1 cut(s) 467
NspI RCATGY 1 cut(s) 475
NspV TTCGAA 1 cut(s) 429
PagI TCATGA 1 cut(s) 163
PciI ACATGT 1 cut(s) 471
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 487
PctI GAATGC 1 cut(s) 546
PfeI GAWTC 1 cut(s) 390
PkrI GCNGC 7 cut(s) 160, 206, 209, 286, 386, 524, 678
PleI GAGTC 3 cut(s) 26, 298, 870
PpsI GAGTC 3 cut(s) 26, 298, 870
PscI ACATGT 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 183, 279
PspEI GGTNACC 1 cut(s) 187
PspGI CCWGG 2 cut(s) 183, 279
PspN4I GGNNCC 6 cut(s) 28, 58, 521, 621, 675, 812
RsaI GTAC 5 cut(s) 344, 581, 638, 684, 764
RsaNI GTAC 5 cut(s) 343, 580, 637, 683, 763
RseI CAYNNNNRTG 4 cut(s) 227, 470, 614, 671
SaqAI TTAA 2 cut(s) 723, 744
SatI GCNGC 7 cut(s) 159, 205, 208, 285, 385, 523, 677
Sau3AI GATC 2 cut(s) 879, 996
ScaI AGTACT 1 cut(s) 344
SchI GAGTC 3 cut(s) 27, 299, 871
ScrFI CCNGG 3 cut(s) 185, 281, 367
SfaNI GCATC 2 cut(s) 474, 542
SfuI TTCGAA 1 cut(s) 429
SmiMI CAYNNNNRTG 4 cut(s) 227, 470, 614, 671
SmlI CTYRAG 2 cut(s) 796, 805
SmoI CTYRAG 2 cut(s) 796, 805
SspMI CTAG 2 cut(s) 8, 152
StyD4I CCNGG 3 cut(s) 183, 279, 365
StyI CCWWGG 2 cut(s) 120, 222
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 8 cut(s) 39, 69, 269, 581, 654, 732, 941, 975
TaqI TCGA 5 cut(s) 39, 69, 147, 429, 468
TaqII GACCGA 1 cut(s) 95
TatI WGTACW 3 cut(s) 342, 636, 762
TauI GCSGC 5 cut(s) 207, 210, 287, 525, 679
TfiI GAWTC 1 cut(s) 390
Tru1I TTAA 2 cut(s) 723, 744
Tru9I TTAA 2 cut(s) 723, 744
TscAI CASTG 1 cut(s) 589
TseFI GTSAC 3 cut(s) 187, 562, 652
TseI GCWGC 2 cut(s) 158, 384
Tsp45I GTSAC 3 cut(s) 187, 562, 652
TspDTI ATGAA 3 cut(s) 502, 701, 804
TspGWI ACGGA 2 cut(s) 39, 996
TspRI CASTG 1 cut(s) 589
XagI CCTNNNNNAGG 1 cut(s) 818
XceI RCATGY 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 753
XspI CTAG 2 cut(s) 8, 152
ZrmI AGTACT 1 cut(s) 344
Zsp2I ATGCAT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.