Rroxscaffold_1G00051600

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000852
Physical Location & Seq
Forward (+)
72259484 .. 72261558
2075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00051600.1

Sequence Viewer

Length: 1467 bp
ATGAACTTTCAAGTGGAGGTTCTCTCAACAGAGACAATCAAACCCTCATCTCCAACCCCTGACCACCTCCGCCACCACAAGCTCTCCTACCTCGACCAAATTCAGCCCCCAATCTTCATGCCCATGATCCTCTTCTACCCGAGAAAACCCGACCACAACGACCTGGACCTCCTTTCCATCCACGAACAGCGTTGCAGCAAAATCAAGAAGGCCTTATCCGACACCTTAGTCAAGTTCTACCCACTAGCCGGAAGAGTCCACGGAACCCAATACGTTGATTGCAACGACGAAGGAGCCTACTACGTCGAAGCCAAGACCGACTGTAAAATCTCTGACATCATCGACAACCCAAACCCTAACGACTTCAATAAGTTCCTCCCTTTCGAGCTAGACGCAGCTCATGAGCTTCCCGTGTGCTTCCAGGTCACCTTCTTCGCCTGCGGCGGCATGAGCATAGCCATGGGGATGTCCCACAAGGTCGGGGACGCCCTCTCCTACTTCACGTTTCTCAATGGCCTGGCCGCTGAGGCTCGTGGGGAGGGCAACGATATTCACCGGCCCGATTTCATCTCAGACAAGTACTTCCCCCAAAAGGACTTATCCGGATTCTTCCAACACCGTAGCGGGATCATAAAGCAAAACATCTCAACAAAACGCTTCGTCTTCGACGCTCCAATGGTTCAGGCCATCCGAGCCAAATGCGTCGACATGTCCCGACGCCCTACTCGCGTTGAAGCTCTATCAGCCTTCATATGGAGCCGCTACATAGCATCATGCACCGGCATGCAACAACATGGTGTCACCACCAACGCGATGTACACTGTTTCTCATGCTGTGAACCTCCGCACAAGAATGGAGCCACCACTTCCGGAGAACACATTCGGAAACGTGACCCGAACAGCCATTGCTGTGCCTCCTGTACCCGTTGACCCGAAAGACGGGACGCTTCATGGAGTGGTTAATCACGTCAGGGAGGCCATCAAACAAGTAGACAAAGAGTACATAAAGAAACTGCAAGAGGGTGACGGACACTTGAGCTTCTTGAGGAGCCGTACGGAGGAGGTGAAACGCGGAGAGGTGGTGTCGTTTAGCTTCACGAGTCTGTGTAGGTTTCCTATCTATGACGCTGATTTTGGATGGGGAAAGCCTGTTTATGTTGGTTCTGCCAGCTTTACGTTCAAGAACTTGGTTAGTTTCTTTGACACTAACGTAGGGGACGGAGTTGAGGCGTGGATCAATCTGTTGGAGGAAGACATGAAAAAATTCGAGGCGGACGAGGAGCTTCTCAAATATGTTTCGCCCGGCCCAGCCCCACTTACATATTACGACTCATTTCTCACTCATGCACATGAAAACTTTACAAGAAAAACTAAAACGATGTGCTTGTCCTTATCAAAATCGAAAAATGGAGCTGGAAATTTTAACAAATGGATACATATGCATGCTGTGTATATGCAAGAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

488

Amino Acids

55.31

Weight (kDa)

6.83

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 428 1.9e-79 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
AccI GTMKAC 2 cut(s) 705, 990
AccII CGCG 3 cut(s) 729, 812, 1071
AccIII TCCGGA 2 cut(s) 602, 868
AciI CCGC 9 cut(s) 70, 441, 444, 522, 624, 760, 844, 1071, 1271
AclWI GGATC 3 cut(s) 121, 635, 1241
AcoI YGGCCR 1 cut(s) 519
AcsI RAATTY 3 cut(s) 99, 1262, 1417
AcyI GRCGYC 2 cut(s) 486, 718
AfaI GTAC 5 cut(s) 581, 818, 921, 1001, 1054
AfiI CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1057
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 4 cut(s) 11, 367, 734, 1180
AjiI CACGTC 1 cut(s) 967
AjnI CCWGG 3 cut(s) 162, 420, 516
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 3 cut(s) 121, 635, 1241
Ama87I CYCGRG 1 cut(s) 139
Aor13HI TCCGGA 2 cut(s) 602, 868
AoxI GGCC 7 cut(s) 210, 514, 519, 557, 684, 975, 1303
ApeKI GCWGC 2 cut(s) 195, 395
ApoI RAATTY 3 cut(s) 99, 1262, 1417
ArsI GACNNNNNNTTYG 2 cut(s) 1115, 1147
Asp700I GAANNNNTTC 2 cut(s) 878, 1262
AspS9I GGNCC 3 cut(s) 166, 558, 1304
AsuC2I CCSGG 1 cut(s) 1302
AsuHPI GGTGA 5 cut(s) 418, 545, 793, 1034, 1075
AvaI CYCGRG 1 cut(s) 139
AvaII GGWCC 1 cut(s) 166
BauI CACGAG 2 cut(s) 531, 1096
BbsI GAAGAC 2 cut(s) 655, 1257
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 2 cut(s) 207, 407
BccI CCATC 4 cut(s) 185, 695, 986, 1131
BceAI ACGGC 1 cut(s) 1035
BcgI CGANNNNNNTGC 2 cut(s) 681, 715
BciT130I CCWGG 3 cut(s) 164, 422, 518
BciVI GTATCC 1 cut(s) 1425
BcnI CCSGG 1 cut(s) 1302
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 2 cut(s) 245, 389
BfuI GTATCC 1 cut(s) 1425
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 6 cut(s) 196, 396, 442, 445, 522, 760
BlsI GCNGC 6 cut(s) 197, 397, 443, 446, 523, 761
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 4 cut(s) 164, 422, 518, 1302
Bme18I GGWCC 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 139
BmgBI CACGTC 1 cut(s) 967
BmgT120I GGNCC 3 cut(s) 166, 558, 1304
BmiI GGNNCC 5 cut(s) 265, 295, 758, 858, 1049
BmrFI CCNGG 4 cut(s) 164, 422, 518, 1302
BmsI GCATC 1 cut(s) 779
BpiI GAAGAC 2 cut(s) 655, 1257
BplI GAGNNNNNCTC 2 cut(s) 8, 40
Bpu10I CCTNAGC 1 cut(s) 525
BpuEI CTTGAG 2 cut(s) 1054, 1063
BpuMI CCSGG 1 cut(s) 1302
BsaHI GRCGYC 2 cut(s) 486, 718
BsaJI CCNNGG 2 cut(s) 259, 459
BsaWI WCCGGW 2 cut(s) 602, 868
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 476, 506, 1065, 1095
Bsc4I CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1057
Bse118I RCCGGY 2 cut(s) 555, 779
Bse3DI GCAATG 1 cut(s) 903
BseAI TCCGGA 2 cut(s) 602, 868
BseBI CCWGG 3 cut(s) 164, 422, 518
BseDI CCNNGG 2 cut(s) 259, 459
BseGI GGATG 4 cut(s) 177, 471, 687, 1142
BseLI CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1057
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 2 cut(s) 516, 585
BseRI GAGGAG 3 cut(s) 1060, 1073, 1292
BseXI GCAGC 2 cut(s) 207, 407
BseYI CCCAGC 1 cut(s) 1306
Bsh1236I CGCG 3 cut(s) 729, 812, 1071
BshFI GGCC 7 cut(s) 212, 516, 521, 559, 686, 977, 1305
BsiHKCI CYCGRG 1 cut(s) 139
BsiSI CCGG 6 cut(s) 249, 556, 603, 780, 869, 1302
BsiWI CGTACG 1 cut(s) 1052
BslFI GGGAC 5 cut(s) 454, 497, 697, 955, 1229
BslI CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1057
BsmAI GTCTC 1 cut(s) 26
BsmFI GGGAC 5 cut(s) 454, 497, 697, 955, 1229
BsnI GGCC 7 cut(s) 212, 516, 521, 559, 686, 977, 1305
BsoBI CYCGRG 1 cut(s) 139
Bsp13I TCCGGA 2 cut(s) 602, 868
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 3 cut(s) 126, 627, 1233
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 9 cut(s) 70, 441, 444, 522, 624, 760, 844, 1071, 1271
BspANI GGCC 7 cut(s) 212, 516, 521, 559, 686, 977, 1305
BspCNI CTCAG 2 cut(s) 517, 584
BspEI TCCGGA 2 cut(s) 602, 868
BspFNI CGCG 3 cut(s) 729, 812, 1071
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 5 cut(s) 265, 295, 758, 858, 1049
BspPI GGATC 3 cut(s) 121, 635, 1241
BsrDI GCAATG 1 cut(s) 903
BsrFI RCCGGY 2 cut(s) 555, 779
BsrGI TGTACA 1 cut(s) 816
BssAI RCCGGY 2 cut(s) 555, 779
BssECI CCNNGG 2 cut(s) 259, 459
BssMI GATC 3 cut(s) 126, 627, 1233
BssNI GRCGYC 2 cut(s) 486, 718
BssSI CACGAG 2 cut(s) 531, 1096
BssT1I CCWWGG 1 cut(s) 459
Bst2BI CACGAG 2 cut(s) 531, 1096
Bst2UI CCWGG 3 cut(s) 164, 422, 518
Bst4CI ACNGT 4 cut(s) 323, 620, 823, 1464
Bst6I CTCTTC 2 cut(s) 137, 247
BstACI GRCGYC 2 cut(s) 486, 718
BstAUI TGTACA 1 cut(s) 816
BstC8I GCNNGC 4 cut(s) 439, 785, 1168, 1443
BstDEI CTNAG 3 cut(s) 226, 525, 571
BstDSI CCRYGG 2 cut(s) 259, 459
BstEII GGTNACC 1 cut(s) 424
BstF5I GGATG 4 cut(s) 177, 471, 687, 1142
BstFNI CGCG 3 cut(s) 729, 812, 1071
BstKTI GATC 3 cut(s) 129, 630, 1236
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 3 cut(s) 126, 627, 1233
BstMWI GCNNNNNNNGC 5 cut(s) 450, 527, 692, 726, 743
BstNI CCWGG 3 cut(s) 164, 422, 518
BstNSI RCATGY 3 cut(s) 712, 787, 1445
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 4 cut(s) 162, 420, 516, 1300
BstUI CGCG 3 cut(s) 729, 812, 1071
BstV1I GCAGC 2 cut(s) 207, 407
BstV2I GAAGAC 2 cut(s) 655, 1257
BsuI GTATCC 1 cut(s) 1425
BsuRI GGCC 7 cut(s) 212, 516, 521, 559, 686, 977, 1305
BtgI CCRYGG 2 cut(s) 259, 459
BtgZI GCGATG 1 cut(s) 827
BtrI CACGTC 1 cut(s) 967
BtsCI GGATG 4 cut(s) 177, 471, 687, 1142
BtsIMutI CAGTG 1 cut(s) 819
Cac8I GCNNGC 4 cut(s) 439, 785, 1168, 1443
CciI TCATGA 1 cut(s) 400
Cfr10I RCCGGY 2 cut(s) 555, 779
Cfr13I GGNCC 3 cut(s) 166, 558, 1304
CseI GACGC 7 cut(s) 401, 494, 677, 691, 726, 952, 1133
Csp6I GTAC 5 cut(s) 580, 817, 920, 1000, 1053
CviQI GTAC 5 cut(s) 580, 817, 920, 1000, 1053
DdeI CTNAG 3 cut(s) 226, 525, 571
DpnI GATC 3 cut(s) 128, 629, 1235
DpnII GATC 3 cut(s) 126, 627, 1233
DrdI GACNNNNNNGTC 1 cut(s) 227
DseDI GACNNNNNNGTC 1 cut(s) 227
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 137, 247
EarI CTCTTC 2 cut(s) 137, 247
EciI GGCGGA 2 cut(s) 59, 1286
Eco130I CCWWGG 1 cut(s) 459
Eco147I AGGCCT 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 166
Eco88I CYCGRG 1 cut(s) 139
Eco91I GGTNACC 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 3 cut(s) 162, 420, 516
EcoT14I CCWWGG 1 cut(s) 459
EcoT22I ATGCAT 1 cut(s) 1443
ErhI CCWWGG 1 cut(s) 459
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 5 cut(s) 454, 497, 697, 955, 1229
FauI CCCGC 1 cut(s) 617
FauNDI CATATG 2 cut(s) 752, 1437
FblI GTMKAC 2 cut(s) 705, 990
Fnu4HI GCNGC 6 cut(s) 196, 396, 442, 445, 522, 760
FokI GGATG 4 cut(s) 164, 478, 674, 1149
Fsp4HI GCNGC 6 cut(s) 196, 396, 442, 445, 522, 760
FspBI CTAG 2 cut(s) 245, 389
GluI GCNGC 6 cut(s) 196, 396, 442, 445, 522, 760
GsaI CCCAGC 1 cut(s) 1310
HaeIII GGCC 7 cut(s) 212, 516, 521, 559, 686, 977, 1305
HapII CCGG 6 cut(s) 249, 556, 603, 780, 869, 1302
HgaI GACGC 7 cut(s) 401, 494, 677, 691, 726, 952, 1133
Hin1I GRCGYC 2 cut(s) 486, 718
HincII GTYRAC 2 cut(s) 706, 928
HindII GTYRAC 2 cut(s) 706, 928
HinfI GANTC 4 cut(s) 255, 606, 1099, 1328
HpaII CCGG 6 cut(s) 249, 556, 603, 780, 869, 1302
HphI GGTGA 5 cut(s) 418, 545, 793, 1034, 1075
Hpy166II GTNNAC 6 cut(s) 259, 706, 819, 838, 928, 991
Hpy188I TCNGA 5 cut(s) 220, 334, 574, 692, 884
Hpy188III TCNNGA 8 cut(s) 205, 401, 603, 714, 869, 1042, 1096, 1180
Hpy8I GTNNAC 6 cut(s) 259, 706, 819, 838, 928, 991
Hpy99I CGWCG 5 cut(s) 290, 308, 671, 707, 720
HpyAV CCTTC 4 cut(s) 202, 284, 439, 757
HpyCH4III ACNGT 4 cut(s) 323, 620, 823, 1464
HpyCH4IV ACGT 7 cut(s) 273, 303, 503, 888, 966, 1175, 1209
HpyCH4V TGCA 8 cut(s) 195, 282, 777, 787, 1015, 1346, 1441, 1456
HpyF10VI GCNNNNNNNGC 5 cut(s) 450, 527, 692, 726, 743
HpyF3I CTNAG 3 cut(s) 226, 525, 571
HpySE526I ACGT 7 cut(s) 273, 303, 503, 888, 966, 1175, 1209
Hsp92I GRCGYC 2 cut(s) 486, 718
Kpn2I TCCGGA 2 cut(s) 602, 868
Kzo9I GATC 3 cut(s) 126, 627, 1233
LmnI GCTCC 7 cut(s) 293, 676, 756, 856, 1047, 1279, 1409
Lsp1109I GCAGC 2 cut(s) 207, 407
LweI GCATC 1 cut(s) 779
MaeI CTAG 2 cut(s) 245, 389
MaeII ACGT 7 cut(s) 273, 303, 503, 888, 966, 1175, 1209
MaeIII GTNAC 4 cut(s) 424, 799, 889, 1022
MalI GATC 3 cut(s) 128, 629, 1235
MboI GATC 3 cut(s) 126, 627, 1233
MboII GAAGA 7 cut(s) 106, 124, 264, 424, 601, 655, 1262
MluCI AATT 3 cut(s) 99, 1262, 1417
MlyI GAGTC 3 cut(s) 264, 1108, 1322
MmeI TCCRAC 4 cut(s) 77, 243, 637, 1224
Mph1103I ATGCAT 1 cut(s) 1443
MroI TCCGGA 2 cut(s) 602, 868
MroXI GAANNNNTTC 2 cut(s) 878, 1262
MseI TTAA 2 cut(s) 960, 1422
MslI CAYNNNNRTG 8 cut(s) 122, 458, 464, 782, 851, 908, 1347, 1440
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 6 cut(s) 249, 556, 603, 780, 869, 1302
MspR9I CCNGG 4 cut(s) 164, 422, 518, 1302
MvaI CCWGG 3 cut(s) 164, 422, 518
MvnI CGCG 3 cut(s) 729, 812, 1071
MwoI GCNNNNNNNGC 5 cut(s) 450, 527, 692, 726, 743
NciI CCSGG 1 cut(s) 1302
NcoI CCATGG 1 cut(s) 459
NdeI CATATG 2 cut(s) 752, 1437
NdeII GATC 3 cut(s) 126, 627, 1233
NlaIV GGNNCC 5 cut(s) 265, 295, 758, 858, 1049
NmuCI GTSAC 4 cut(s) 424, 799, 889, 1022
NsiI ATGCAT 1 cut(s) 1443
NspI RCATGY 3 cut(s) 712, 787, 1445
PaeI GCATGC 2 cut(s) 787, 1445
PagI TCATGA 1 cut(s) 400
PceI AGGCCT 1 cut(s) 212
PciI ACATGT 1 cut(s) 708
PcsI WCGNNNNNNNCGW 1 cut(s) 1272
PdmI GAANNNNTTC 2 cut(s) 878, 1262
PfeI GAWTC 1 cut(s) 606
Pfl23II CGTACG 1 cut(s) 1052
PkrI GCNGC 6 cut(s) 197, 397, 443, 446, 523, 761
PleI GAGTC 3 cut(s) 263, 1107, 1322
PpsI GAGTC 3 cut(s) 263, 1107, 1322
PscI ACATGT 1 cut(s) 708
Psp6I CCWGG 3 cut(s) 162, 420, 516
PspEI GGTNACC 1 cut(s) 424
PspFI CCCAGC 1 cut(s) 1306
PspGI CCWGG 3 cut(s) 162, 420, 516
PspLI CGTACG 1 cut(s) 1052
PspN4I GGNNCC 5 cut(s) 265, 295, 758, 858, 1049
PspPI GGNCC 3 cut(s) 166, 558, 1304
RsaI GTAC 5 cut(s) 581, 818, 921, 1001, 1054
RsaNI GTAC 5 cut(s) 580, 817, 920, 1000, 1053
RseI CAYNNNNRTG 8 cut(s) 122, 458, 464, 782, 851, 908, 1347, 1440
SalI GTCGAC 1 cut(s) 704
SaqAI TTAA 2 cut(s) 960, 1422
SatI GCNGC 6 cut(s) 196, 396, 442, 445, 522, 760
Sau3AI GATC 3 cut(s) 126, 627, 1233
Sau96I GGNCC 3 cut(s) 166, 558, 1304
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 3 cut(s) 264, 1108, 1322
ScrFI CCNGG 4 cut(s) 164, 422, 518, 1302
SfaNI GCATC 1 cut(s) 779
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 8 cut(s) 122, 458, 464, 782, 851, 908, 1347, 1440
SmlI CTYRAG 2 cut(s) 1033, 1042
SmoI CTYRAG 2 cut(s) 1033, 1042
SphI GCATGC 2 cut(s) 787, 1445
Sse9I AATT 3 cut(s) 99, 1262, 1417
SseBI AGGCCT 1 cut(s) 212
SsiI CCGC 9 cut(s) 70, 441, 444, 522, 624, 760, 844, 1071, 1271
SspMI CTAG 2 cut(s) 245, 389
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 4 cut(s) 162, 420, 516, 1300
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 4 cut(s) 323, 620, 823, 1464
TaiI ACGT 7 cut(s) 276, 306, 506, 891, 969, 1178, 1212
TaqI TCGA 8 cut(s) 93, 306, 342, 384, 666, 705, 1266, 1400
TaqII GACCGA 1 cut(s) 332
TasI AATT 3 cut(s) 99, 1262, 1417
TatI WGTACW 3 cut(s) 579, 816, 999
TauI GCSGC 4 cut(s) 444, 447, 524, 762
TfiI GAWTC 1 cut(s) 606
Tru1I TTAA 2 cut(s) 960, 1422
Tru9I TTAA 2 cut(s) 960, 1422
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 4 cut(s) 424, 799, 889, 1022
TseI GCWGC 2 cut(s) 195, 395
Tsp45I GTSAC 4 cut(s) 424, 799, 889, 1022
TspDTI ATGAA 7 cut(s) 17, 106, 556, 739, 938, 1271, 1365
TspGWI ACGGA 4 cut(s) 276, 1041, 1070, 1233
TspRI CASTG 1 cut(s) 826
VpaK11BI GGWCC 1 cut(s) 166
XapI RAATTY 3 cut(s) 99, 1262, 1417
XceI RCATGY 3 cut(s) 712, 787, 1445
XmiI GTMKAC 2 cut(s) 705, 990
XmnI GAANNNNTTC 2 cut(s) 878, 1262
XspI CTAG 2 cut(s) 245, 389
ZrmI AGTACT 1 cut(s) 581
Zsp2I ATGCAT 1 cut(s) 1443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.