pycom13g09440

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000852
Physical Location & Seq
Forward (+)
6316883 .. 6318508
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g09440.3

Sequence Viewer

Length: 1413 bp
ATGACTAAATTGGAAGTGGAGGTTGTCTCAACAGAGACCATCAAACCCTCATCTCCAACCCCAGACCACCTCCGCCGCGATAACCTCTCCTTTCTTGACCAAATCCAACCCCCAATATTCATACCCCACGGAACGCTGCACGAAAATCAAAACCGCCTTATATCGGAAACCCTGACCAATTTCTACCCGTTAGCCGGGCGAGTAAGAGGCACCCAATACGTCGATTGCAACGACGAGGGTGCCTACTACGTGGAAGCCAAAGCCAACTCCAACATCATCGACATCATCCAAAACCCTAACCCTATCGACTTCAACAAGTTCATACTGTGTGCGCTCGACGCTGCTAGCGAACTCCCCATATGCCTACAAGTTACGTTCTTTGCATGCGGAGGCATGAGCATCGGTATGGGCATGTCGCACAAGGTTGGAGACGCCCTGTCATATTTCATGTTCCTCAACAGTCTCGCCGCCACTGCTCGCGGGGATACTTCCCCCAACAGGACTTGTCCGGGTCCTACCAGCATAGTAGCGGGATCGATCAAGCACAACATTTCCACTAAGCGCTTTGTGTTCAACGCTCCCGCGGTTTCCGCTCTCCGAGCCCAATCTACGGACGTCGAAAACGAACAAGTGAGGCGGCCTACACGTGTGGAGGCCTTATCGGCTTTCATATGGAGACGATCGCGGCCTGCACCAGCCAACAACAAAACAACAGCGCTGACGAGGTCCCAAATGGACCCACCGCTTCCGGAGTATACATTCGGGAATGTGACCCGAACAACAATCTCCGTCCCACCCATACAGGTGGAACCGAAAGAAGGATCATCATCTGACCCTTCTCTATATTCCGTAGTGAGCCACGTCAGAGAGGCTCTTAAACAAGTTGACAGAGAGTACATAAAGAAACTGCAAGAGGGGGAGGGGTTCTTGAGTTTCTTGAAAGAGCGGAGCTCACAGGTGAAACGTGAAGAGGTGGTGTCGTTTAGCTTCACCAGCTTGTGTAGGTTTCCAATTTACGATGCTGATTTTGGGTGGGGGAAGCCTGTTTATGCCGGGTCCGCGAGTTTTACATTTAAGAATTTAGTGAGCTTCTTTGACACAACAAGTGGCGACCGTGTGGAGGCTTGGATTAACTTGAAGGAGGAAGACATGGAAAAATTTGAAGCTGATGAGGAGGTTCTCAAGTATGTTTCACCCAACCTGAGTTTGTATGCATTTAATTTGTTACTTTACAGTAATTTCTCTGAGTTCTATTTCATTACGAAAAATAATAGAGATATTATGGTTGAATTCTATTTCTTTAATGATTTAAAGAAGAAGTTTAACCATCAATCGCCACATTATATGATCTATCAAAACATAGTTCAAGTAAATAGTCTCTCTACCATTATCCTTTTGTTAATGTGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

471

Amino Acids

53.08

Weight (kDa)

5.98

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 618
AccB1I GGYRCC 2 cut(s) 209, 239
AccBSI CCGCTC 2 cut(s) 593, 946
AccI GTMKAC 1 cut(s) 755
AccII CGCG 5 cut(s) 78, 480, 584, 685, 1061
AccIII TCCGGA 1 cut(s) 748
AclWI GGATC 2 cut(s) 541, 829
AcsI RAATTY 3 cut(s) 1078, 1157, 1289
AcvI CACGTG 1 cut(s) 647
AcyI GRCGYC 2 cut(s) 432, 615
AfaI GTAC 1 cut(s) 896
AfeI AGCGCT 2 cut(s) 563, 717
AfiI CCNNNNNNNGG 6 cut(s) 163, 194, 498, 610, 818, 1120
AflIII ACRYGT 2 cut(s) 644, 646
AgsI TTSAA 7 cut(s) 313, 574, 940, 1138, 1163, 1289, 1367
AhdI GACNNNNNGTC 1 cut(s) 436
AjiI CACGTC 1 cut(s) 862
AluBI AGCT 5 cut(s) 951, 987, 996, 1089, 1166
AluI AGCT 5 cut(s) 951, 987, 996, 1089, 1166
Alw21I GWGCWC 1 cut(s) 953
Alw26I GTCTC 6 cut(s) 29, 31, 423, 467, 670, 1382
AlwI GGATC 2 cut(s) 541, 829
Aor13HI TCCGGA 1 cut(s) 748
Aor51HI AGCGCT 2 cut(s) 563, 717
AoxI GGCC 3 cut(s) 638, 654, 686
ApeKI GCWGC 2 cut(s) 136, 341
ApoI RAATTY 3 cut(s) 1078, 1157, 1289
AspLEI GCGC 3 cut(s) 334, 564, 718
AspS9I GGNCC 4 cut(s) 512, 726, 736, 1056
AsuC2I CCSGG 3 cut(s) 196, 510, 1054
AsuHPI GGTGA 3 cut(s) 970, 982, 1185
AsuNHI GCTAGC 1 cut(s) 344
AvaII GGWCC 4 cut(s) 512, 726, 736, 1056
BanI GGYRCC 2 cut(s) 209, 239
BanII GRGCYC 2 cut(s) 604, 953
BbrPI CACGTG 1 cut(s) 647
BbsI GAAGAC 1 cut(s) 1152
Bbv12I GWGCWC 1 cut(s) 953
BbvI GCAGC 2 cut(s) 123, 328
BccI CCATC 2 cut(s) 47, 1335
BcgI CGANNNNNNTGC 4 cut(s) 221, 255, 382, 416
BciVI GTATCC 1 cut(s) 478
BcnI CCSGG 3 cut(s) 196, 510, 1054
BcoDI GTCTC 6 cut(s) 29, 31, 423, 467, 670, 1382
BfaI CTAG 1 cut(s) 345
BfoI RGCGCY 2 cut(s) 565, 719
BfuI GTATCC 1 cut(s) 478
BglI GCCNNNNNGGC 1 cut(s) 662
BisI GCNGC 6 cut(s) 76, 137, 342, 468, 638, 686
BlsI GCNGC 6 cut(s) 77, 138, 343, 469, 639, 687
Bme1390I CCNGG 3 cut(s) 196, 510, 1054
Bme18I GGWCC 4 cut(s) 512, 726, 736, 1056
BmeRI GACNNNNNGTC 1 cut(s) 436
BmgBI CACGTC 1 cut(s) 862
BmgT120I GGNCC 4 cut(s) 512, 726, 736, 1056
BmiI GGNNCC 7 cut(s) 211, 241, 513, 728, 738, 810, 1057
BmrFI CCNGG 3 cut(s) 196, 510, 1054
BmsI GCATC 2 cut(s) 408, 1009
BmtI GCTAGC 1 cut(s) 348
BpiI GAAGAC 1 cut(s) 1152
BplI GAGNNNNNCTC 4 cut(s) 11, 43, 935, 967
BpuEI CTTGAG 2 cut(s) 949, 1166
BpuMI CCSGG 3 cut(s) 196, 510, 1054
Bsa29I ATCGAT 1 cut(s) 536
BsaAI YACGTR 2 cut(s) 250, 647
BsaBI GATNNNNATC 1 cut(s) 826
BsaHI GRCGYC 2 cut(s) 432, 615
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 127, 582
BsaWI WCCGGW 1 cut(s) 748
Bsc4I CCNNNNNNNGG 6 cut(s) 163, 194, 498, 610, 818, 1120
Bse8I GATNNNNATC 1 cut(s) 826
BseAI TCCGGA 1 cut(s) 748
BseCI ATCGAT 1 cut(s) 536
BseDI CCNNGG 2 cut(s) 127, 582
BseGI GGATG 1 cut(s) 285
BseJI GATNNNNATC 1 cut(s) 826
BseLI CCNNNNNNNGG 6 cut(s) 163, 194, 498, 610, 818, 1120
BseMII CTCAG 2 cut(s) 1193, 1236
BseRI GAGGAG 1 cut(s) 1187
BseXI GCAGC 2 cut(s) 123, 328
BsgI GTGCAG 2 cut(s) 122, 675
Bsh1236I CGCG 5 cut(s) 78, 480, 584, 685, 1061
Bsh1285I CGRYCG 2 cut(s) 683, 1114
BshFI GGCC 3 cut(s) 640, 656, 688
BshNI GGYRCC 2 cut(s) 209, 239
BshVI ATCGAT 1 cut(s) 536
BsiEI CGRYCG 2 cut(s) 683, 1114
BsiHKAI GWGCWC 1 cut(s) 953
BsiSI CCGG 4 cut(s) 195, 509, 749, 1053
BslFI GGGAC 2 cut(s) 712, 776
BslI CCNNNNNNNGG 6 cut(s) 163, 194, 498, 610, 818, 1120
BsmAI GTCTC 6 cut(s) 29, 31, 423, 467, 670, 1382
BsmBI CGTCTC 2 cut(s) 423, 670
BsmFI GGGAC 2 cut(s) 712, 776
BsnI GGCC 3 cut(s) 640, 656, 688
Bso31I GGTCTC 1 cut(s) 29
Bsp1286I GDGCHC 2 cut(s) 604, 953
Bsp13I TCCGGA 1 cut(s) 748
Bsp143I GATC 5 cut(s) 533, 537, 680, 821, 1347
BspANI GGCC 3 cut(s) 640, 656, 688
BspCNI CTCAG 2 cut(s) 1194, 1237
BspDI ATCGAT 1 cut(s) 536
BspEI TCCGGA 1 cut(s) 748
BspFNI CGCG 5 cut(s) 78, 480, 584, 685, 1061
BspLI GGNNCC 7 cut(s) 211, 241, 513, 728, 738, 810, 1057
BspOI GCTAGC 1 cut(s) 348
BspPI GGATC 2 cut(s) 541, 829
BspT107I GGYRCC 2 cut(s) 209, 239
BspTNI GGTCTC 1 cut(s) 29
BsrBI CCGCTC 2 cut(s) 593, 946
BssECI CCNNGG 2 cut(s) 127, 582
BssMI GATC 5 cut(s) 533, 537, 680, 821, 1347
BssNAI GTATAC 1 cut(s) 756
BssNI GRCGYC 2 cut(s) 432, 615
Bst1107I GTATAC 1 cut(s) 756
Bst4CI ACNGT 4 cut(s) 327, 461, 1115, 1235
Bst6I CTCTTC 1 cut(s) 963
BstACI GRCGYC 2 cut(s) 432, 615
BstBAI YACGTR 2 cut(s) 250, 647
BstC8I GCNNGC 4 cut(s) 346, 385, 478, 690
BstDEI CTNAG 3 cut(s) 558, 1202, 1245
BstDSI CCRYGG 2 cut(s) 127, 582
BstF5I GGATG 1 cut(s) 285
BstFNI CGCG 5 cut(s) 78, 480, 584, 685, 1061
BstH2I RGCGCY 2 cut(s) 565, 719
BstHHI GCGC 3 cut(s) 334, 564, 718
BstKTI GATC 5 cut(s) 536, 540, 683, 824, 1350
BstMAI GTCTC 6 cut(s) 29, 31, 423, 467, 670, 1382
BstMBI GATC 5 cut(s) 533, 537, 680, 821, 1347
BstMCI CGRYCG 2 cut(s) 683, 1114
BstMWI GCNNNNNNNGC 7 cut(s) 338, 473, 590, 599, 662, 993, 1058
BstNSI RCATGY 2 cut(s) 387, 415
BstSCI CCNGG 3 cut(s) 194, 508, 1052
BstUI CGCG 5 cut(s) 78, 480, 584, 685, 1061
BstV1I GCAGC 2 cut(s) 123, 328
BstV2I GAAGAC 1 cut(s) 1152
BstXI CCANNNNNNTGG 1 cut(s) 805
BstZ17I GTATAC 1 cut(s) 756
Bsu15I ATCGAT 1 cut(s) 536
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 3 cut(s) 640, 656, 688
BsuTUI ATCGAT 1 cut(s) 536
BtgI CCRYGG 2 cut(s) 127, 582
BtrI CACGTC 1 cut(s) 862
BtsCI GGATG 1 cut(s) 285
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 1 cut(s) 471
Cac8I GCNNGC 4 cut(s) 346, 385, 478, 690
CfoI GCGC 3 cut(s) 334, 564, 718
Cfr13I GGNCC 4 cut(s) 512, 726, 736, 1056
Cfr42I CCGCGG 1 cut(s) 585
ClaI ATCGAT 1 cut(s) 536
CseI GACGC 2 cut(s) 347, 440
Csp6I GTAC 1 cut(s) 895
CviAII CATG 5 cut(s) 384, 394, 412, 448, 1150
CviQI GTAC 1 cut(s) 895
DdeI CTNAG 3 cut(s) 558, 1202, 1245
DpnI GATC 5 cut(s) 535, 539, 682, 823, 1349
DpnII GATC 5 cut(s) 533, 537, 680, 821, 1347
DraI TTTAAA 1 cut(s) 1311
DriI GACNNNNNGTC 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 963
Eam1105I GACNNNNNGTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 963
EciI GGCGGA 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 951
Eco147I AGGCCT 1 cut(s) 656
Eco24I GRGCYC 2 cut(s) 604, 953
Eco31I GGTCTC 1 cut(s) 29
Eco47I GGWCC 4 cut(s) 512, 726, 736, 1056
Eco47III AGCGCT 2 cut(s) 563, 717
Eco53kI GAGCTC 1 cut(s) 951
Eco72I CACGTG 1 cut(s) 647
EcoICRI GAGCTC 1 cut(s) 951
EcoO109I RGGNCCY 2 cut(s) 512, 726
EcoRI GAATTC 1 cut(s) 1289
EcoT22I ATGCAT 1 cut(s) 1216
EcoT38I GRGCYC 2 cut(s) 604, 953
Esp3I CGTCTC 2 cut(s) 423, 670
FaeI CATG 5 cut(s) 387, 397, 415, 451, 1153
FaqI GGGAC 2 cut(s) 712, 776
FatI CATG 5 cut(s) 383, 393, 411, 447, 1149
FauI CCCGC 3 cut(s) 473, 523, 589
FauNDI CATATG 2 cut(s) 359, 671
FblI GTMKAC 1 cut(s) 755
Fnu4HI GCNGC 6 cut(s) 76, 137, 342, 468, 638, 686
FokI GGATG 1 cut(s) 272
FriOI GRGCYC 2 cut(s) 604, 953
Fsp4HI GCNGC 6 cut(s) 76, 137, 342, 468, 638, 686
FspBI CTAG 1 cut(s) 345
GlaI GCGC 3 cut(s) 333, 563, 717
GluI GCNGC 6 cut(s) 76, 137, 342, 468, 638, 686
HaeII RGCGCY 2 cut(s) 565, 719
HaeIII GGCC 3 cut(s) 640, 656, 688
HapII CCGG 4 cut(s) 195, 509, 749, 1053
HgaI GACGC 2 cut(s) 347, 440
HhaI GCGC 3 cut(s) 334, 564, 718
Hin1I GRCGYC 2 cut(s) 432, 615
Hin1II CATG 5 cut(s) 387, 397, 415, 451, 1153
Hin6I GCGC 3 cut(s) 332, 562, 716
HinP1I GCGC 3 cut(s) 332, 562, 716
HincII GTYRAC 1 cut(s) 886
HindII GTYRAC 1 cut(s) 886
HpaII CCGG 4 cut(s) 195, 509, 749, 1053
HphI GGTGA 3 cut(s) 970, 982, 1185
Hpy166II GTNNAC 2 cut(s) 756, 886
Hpy188I TCNGA 5 cut(s) 166, 599, 832, 866, 1246
Hpy188III TCNNGA 5 cut(s) 95, 749, 763, 928, 937
Hpy8I GTNNAC 2 cut(s) 756, 886
Hpy99I CGWCG 4 cut(s) 224, 236, 341, 620
HpyAV CCTTC 3 cut(s) 812, 846, 1132
HpyCH4III ACNGT 4 cut(s) 327, 461, 1115, 1235
HpyCH4IV ACGT 7 cut(s) 219, 249, 374, 615, 646, 861, 964
HpyCH4V TGCA 6 cut(s) 139, 228, 383, 692, 910, 1214
HpyF10VI GCNNNNNNNGC 7 cut(s) 338, 473, 590, 599, 662, 993, 1058
HpyF3I CTNAG 3 cut(s) 558, 1202, 1245
HpySE526I ACGT 7 cut(s) 219, 249, 374, 615, 646, 861, 964
Hsp92I GRCGYC 2 cut(s) 432, 615
Hsp92II CATG 5 cut(s) 387, 397, 415, 451, 1153
HspAI GCGC 3 cut(s) 332, 562, 716
Kpn2I TCCGGA 1 cut(s) 748
KspI CCGCGG 1 cut(s) 585
Kzo9I GATC 5 cut(s) 533, 537, 680, 821, 1347
LmnI GCTCC 2 cut(s) 583, 948
Lsp1109I GCAGC 2 cut(s) 123, 328
LweI GCATC 2 cut(s) 408, 1009
MaeI CTAG 1 cut(s) 345
MaeII ACGT 7 cut(s) 219, 249, 374, 615, 646, 861, 964
MaeIII GTNAC 3 cut(s) 370, 769, 1224
MalI GATC 5 cut(s) 535, 539, 682, 823, 1349
MbiI CCGCTC 2 cut(s) 593, 946
MboI GATC 5 cut(s) 533, 537, 680, 821, 1347
MboII GAAGA 3 cut(s) 980, 1157, 1327
MhlI GDGCHC 2 cut(s) 604, 953
MluCI AATT 8 cut(s) 8, 178, 1011, 1078, 1157, 1219, 1237, 1289
MmeI TCCRAC 4 cut(s) 80, 130, 294, 406
Mph1103I ATGCAT 1 cut(s) 1216
MroI TCCGGA 1 cut(s) 748
MseI TTAA 8 cut(s) 876, 1074, 1131, 1218, 1302, 1310, 1323, 1400
MslI CAYNNNNRTG 2 cut(s) 404, 803
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 4 cut(s) 195, 509, 749, 1053
MspR9I CCNGG 3 cut(s) 196, 510, 1054
MvnI CGCG 5 cut(s) 78, 480, 584, 685, 1061
MwoI GCNNNNNNNGC 7 cut(s) 338, 473, 590, 599, 662, 993, 1058
NciI CCSGG 3 cut(s) 196, 510, 1054
NdeI CATATG 2 cut(s) 359, 671
NdeII GATC 5 cut(s) 533, 537, 680, 821, 1347
NheI GCTAGC 1 cut(s) 344
NlaIII CATG 5 cut(s) 387, 397, 415, 451, 1153
NlaIV GGNNCC 7 cut(s) 211, 241, 513, 728, 738, 810, 1057
NmuCI GTSAC 1 cut(s) 769
NsiI ATGCAT 1 cut(s) 1216
NspI RCATGY 2 cut(s) 387, 415
PaeI GCATGC 1 cut(s) 387
PceI AGGCCT 1 cut(s) 656
PcsI WCGNNNNNNNCGW 3 cut(s) 228, 345, 621
PflFI GACNNNGTC 1 cut(s) 724
PkrI GCNGC 6 cut(s) 77, 138, 343, 469, 639, 687
Ple19I CGATCG 1 cut(s) 683
PmaCI CACGTG 1 cut(s) 647
PmlI CACGTG 1 cut(s) 647
Ppu21I YACGTR 2 cut(s) 250, 647
PpuMI RGGWCCY 2 cut(s) 512, 726
Psp124BI GAGCTC 1 cut(s) 953
Psp5II RGGWCCY 2 cut(s) 512, 726
PspCI CACGTG 1 cut(s) 647
PspN4I GGNNCC 7 cut(s) 211, 241, 513, 728, 738, 810, 1057
PspPI GGNCC 4 cut(s) 512, 726, 736, 1056
PspPPI RGGWCCY 2 cut(s) 512, 726
PsyI GACNNNGTC 1 cut(s) 724
PvuI CGATCG 1 cut(s) 683
RsaI GTAC 1 cut(s) 896
RsaNI GTAC 1 cut(s) 895
RseI CAYNNNNRTG 2 cut(s) 404, 803
SacI GAGCTC 1 cut(s) 953
SacII CCGCGG 1 cut(s) 585
SaqAI TTAA 8 cut(s) 876, 1074, 1131, 1218, 1302, 1310, 1323, 1400
SatI GCNGC 6 cut(s) 76, 137, 342, 468, 638, 686
Sau3AI GATC 5 cut(s) 533, 537, 680, 821, 1347
Sau96I GGNCC 4 cut(s) 512, 726, 736, 1056
ScrFI CCNGG 3 cut(s) 196, 510, 1054
SduI GDGCHC 2 cut(s) 604, 953
SfaNI GCATC 2 cut(s) 408, 1009
Sfr303I CCGCGG 1 cut(s) 585
SgrBI CCGCGG 1 cut(s) 585
SinI GGWCC 4 cut(s) 512, 726, 736, 1056
SmiMI CAYNNNNRTG 2 cut(s) 404, 803
SmlI CTYRAG 2 cut(s) 928, 1181
SmoI CTYRAG 2 cut(s) 928, 1181
SphI GCATGC 1 cut(s) 387
Sse9I AATT 8 cut(s) 8, 178, 1011, 1078, 1157, 1219, 1237, 1289
SseBI AGGCCT 1 cut(s) 656
SspI AATATT 1 cut(s) 117
SspMI CTAG 1 cut(s) 345
SstI GAGCTC 1 cut(s) 953
StuI AGGCCT 1 cut(s) 656
StyD4I CCNGG 3 cut(s) 194, 508, 1052
TaaI ACNGT 4 cut(s) 327, 461, 1115, 1235
TaiI ACGT 7 cut(s) 222, 252, 377, 618, 649, 864, 967
TaqI TCGA 6 cut(s) 222, 279, 306, 336, 536, 618
TasI AATT 8 cut(s) 8, 178, 1011, 1078, 1157, 1219, 1237, 1289
TatI WGTACW 1 cut(s) 894
TauI GCSGC 4 cut(s) 78, 470, 640, 688
Tru1I TTAA 8 cut(s) 876, 1074, 1131, 1218, 1302, 1310, 1323, 1400
Tru9I TTAA 8 cut(s) 876, 1074, 1131, 1218, 1302, 1310, 1323, 1400
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 1 cut(s) 769
TseI GCWGC 2 cut(s) 136, 341
Tsp45I GTSAC 1 cut(s) 769
TspDTI ATGAA 5 cut(s) 109, 310, 436, 658, 1246
TspGWI ACGGA 4 cut(s) 144, 626, 778, 838
TspRI CASTG 1 cut(s) 478
Tth111I GACNNNGTC 1 cut(s) 724
VpaK11BI GGWCC 4 cut(s) 512, 726, 736, 1056
XapI RAATTY 3 cut(s) 1078, 1157, 1289
XceI RCATGY 2 cut(s) 387, 415
XmiI GTMKAC 1 cut(s) 755
XspI CTAG 1 cut(s) 345
ZraI GACGTC 1 cut(s) 616
Zsp2I ATGCAT 1 cut(s) 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.