Rw1G011140

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000852
Physical Location & Seq
Forward (+)
25150541 .. 25151830
1290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011140.1

Sequence Viewer

Length: 1206 bp
ATGGGATGTTTCCTCTCCAACACATCTCCAACCCCTGACCACTTCCGCCACCACAAGCTCTCCTACCTCGACCAAATCTACGAAATCAAAAAGGCCTTATCCGACACCTTAGTCAAGTACTACCCACTAGCCGGAAGAGTCCACGGAACCCAATACGTCGATTGCAACGACGAAGGAGCCTACTACGTCGAAGCCAAGACCGACTGCAAAATCTCTGACATCATCGACAACCCAAACCCTAAGGACTTCAACAAGTTCCTCCCTTTCGAGCTAGACGCAGCTCATGAGCTTCCCGTGTGCTTTCAGGTCACCTTCTTCGCCTGTGGCGGCATGAGCATCGGCACGGGGATGTCCCACAAGGTCGGGGACGCCCTCTCCTACATCACGTTCCTCAATGGCTTGGCCGCTGAGGCTCGTGGGGAGGGCAACGATATTCACCCTCCCGAGTTCGTCTCAGACAAGTACTTCCCCCAAAAGGACTTGTCCAGATTCTTCCAACACCGCACCGGGATCATAAAGCAAAACATCTCAACAAAACGCTTCGTCTTCGACGCTCCAATGGTTCAGGCCATCCGAGCCAAATGCATGGACATGTCCAGACGCCCCACTCGTGTTGAAGCCCTTTCAGCTTTCATATGGAGCCGCTACATAACATCATGCACCGGCATTCAACAAGATAGCGTCACCACCGACGCGATGTACACTGTTTCTCATACTGTGAACCTCCGCACGAGAATGGAGCCACCACTTCCCGAGTACGCATTCGGGAACGTGACCCGAACCGCCATTGCGGTGCCGCCCATACCCGTTGACCCGAAAGATGGGACGCTTCATGGGGTGGTTAATTACGTCAAGGAGGCCATTAAGCAAGTAGACAAAGAGTTCATAAAGAAATTGCAAGAGGGTGACGGACACTTGAGCTTCTTGAGGAGCCGTACGGAGGAGGTGAAACGCGGAGAGGTGGTGTCGTTTAGCTTCACAAGTTTGTGTAGGTTTCCGATCTACGACGCTGATTTGGGATGGGGGAAGCCTATTAATGTTGGTTCTGCCAGCTTTACGTTCAAGAACTTGGTTAGTTTCTTTGACACCAACGTAGGGGATGGAGTTGAGGCGTGGATCAATCTTTTGGAGGAAGACATGGAAAAATTCGAGGCAGACGAGGAGCTTCTGAAGTATGTTTCACCCGCCCCGAGTTCAAAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

401

Amino Acids

45.11

Weight (kDa)

5.81

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 26 - 389 2.6e-67 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccB1I GGYRCC 1 cut(s) 793
AccI GTMKAC 1 cut(s) 873
AccII CGCG 2 cut(s) 695, 954
AclWI GGATC 2 cut(s) 518, 1124
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 1 cut(s) 1145
AcuI CTGAAG 1 cut(s) 1190
AcyI GRCGYC 2 cut(s) 369, 601
AfaI GTAC 5 cut(s) 119, 464, 701, 758, 937
AfiI CCNNNNNNNGG 5 cut(s) 131, 475, 821, 940, 1095
AflIII ACRYGT 1 cut(s) 591
AgsI TTSAA 5 cut(s) 250, 617, 671, 1063, 1197
AluBI AGCT 9 cut(s) 58, 271, 281, 289, 629, 921, 975, 1053, 1165
AluI AGCT 9 cut(s) 58, 271, 281, 289, 629, 921, 975, 1053, 1165
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 2 cut(s) 518, 1124
Ama87I CYCGRG 3 cut(s) 443, 752, 1189
AoxI GGCC 4 cut(s) 93, 402, 567, 858
ApeKI GCWGC 1 cut(s) 278
ApoI RAATTY 1 cut(s) 1145
AseI ATTAAT 1 cut(s) 1035
AsuC2I CCSGG 1 cut(s) 508
AsuHPI GGTGA 6 cut(s) 301, 428, 676, 917, 958, 1173
AvaI CYCGRG 3 cut(s) 443, 752, 1189
AxyI CCTNAGG 1 cut(s) 240
BanI GGYRCC 1 cut(s) 793
BauI CACGAG 3 cut(s) 414, 609, 730
BbsI GAAGAC 2 cut(s) 538, 1140
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 290
BccI CCATC 4 cut(s) 578, 815, 1014, 1094
BceAI ACGGC 1 cut(s) 918
BcgI CGANNNNNNTGC 4 cut(s) 319, 353, 564, 598
BcnI CCSGG 1 cut(s) 508
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 3 cut(s) 128, 272, 1204
BglI GCCNNNNNGGC 1 cut(s) 410
BisI GCNGC 5 cut(s) 279, 328, 405, 643, 797
BlsI GCNGC 5 cut(s) 280, 329, 406, 644, 798
BmcAI AGTACT 2 cut(s) 119, 464
Bme1390I CCNGG 1 cut(s) 508
BmeT110I CYCGRG 3 cut(s) 443, 752, 1189
BmiI GGNNCC 6 cut(s) 148, 178, 641, 741, 795, 932
BmrFI CCNGG 1 cut(s) 508
BmsI GCATC 1 cut(s) 345
BpiI GAAGAC 2 cut(s) 538, 1140
BplI GAGNNNNNCTC 2 cut(s) 437, 469
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 2 cut(s) 937, 946
BpuMI CCSGG 1 cut(s) 508
BsaHI GRCGYC 2 cut(s) 369, 601
BsaJI CCNNGG 1 cut(s) 142
BsaXI ACNNNNNCTCC 6 cut(s) 44, 74, 359, 389, 948, 978
Bsc4I CCNNNNNNNGG 5 cut(s) 131, 475, 821, 940, 1095
Bse118I RCCGGY 1 cut(s) 662
Bse21I CCTNAGG 1 cut(s) 240
Bse3DI GCAATG 1 cut(s) 786
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 5 cut(s) 11, 354, 570, 1025, 1105
BseLI CCNNNNNNNGG 5 cut(s) 131, 475, 821, 940, 1095
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 2 cut(s) 399, 468
BseRI GAGGAG 3 cut(s) 943, 956, 1175
BseXI GCAGC 1 cut(s) 290
Bsh1236I CGCG 2 cut(s) 695, 954
BshFI GGCC 4 cut(s) 95, 404, 569, 860
BshNI GGYRCC 1 cut(s) 793
BsiHKCI CYCGRG 3 cut(s) 443, 752, 1189
BsiSI CCGG 3 cut(s) 132, 507, 663
BsiWI CGTACG 1 cut(s) 935
BslFI GGGAC 3 cut(s) 337, 380, 838
BslI CCNNNNNNNGG 5 cut(s) 131, 475, 821, 940, 1095
BsmAI GTCTC 1 cut(s) 457
BsmBI CGTCTC 1 cut(s) 457
BsmFI GGGAC 3 cut(s) 337, 380, 838
BsmI GAATGC 2 cut(s) 666, 761
BsnI GGCC 4 cut(s) 95, 404, 569, 860
BsoBI CYCGRG 3 cut(s) 443, 752, 1189
Bsp1407I TGTACA 1 cut(s) 699
Bsp143I GATC 3 cut(s) 510, 999, 1116
BspANI GGCC 4 cut(s) 95, 404, 569, 860
BspCNI CTCAG 2 cut(s) 400, 467
BspFNI CGCG 2 cut(s) 695, 954
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 6 cut(s) 148, 178, 641, 741, 795, 932
BspPI GGATC 2 cut(s) 518, 1124
BspT107I GGYRCC 1 cut(s) 793
BsrDI GCAATG 1 cut(s) 786
BsrFI RCCGGY 1 cut(s) 662
BsrGI TGTACA 1 cut(s) 699
BssAI RCCGGY 1 cut(s) 662
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 3 cut(s) 510, 999, 1116
BssNI GRCGYC 2 cut(s) 369, 601
BssSI CACGAG 3 cut(s) 414, 609, 730
Bst2BI CACGAG 3 cut(s) 414, 609, 730
Bst4CI ACNGT 2 cut(s) 706, 718
Bst6I CTCTTC 1 cut(s) 130
BstACI GRCGYC 2 cut(s) 369, 601
BstAUI TGTACA 1 cut(s) 699
BstC8I GCNNGC 1 cut(s) 1051
BstDEI CTNAG 4 cut(s) 109, 240, 408, 454
BstDSI CCRYGG 1 cut(s) 142
BstEII GGTNACC 1 cut(s) 307
BstF5I GGATG 5 cut(s) 11, 354, 570, 1025, 1105
BstFNI CGCG 2 cut(s) 695, 954
BstKTI GATC 3 cut(s) 513, 1002, 1119
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 3 cut(s) 510, 999, 1116
BstMWI GCNNNNNNNGC 4 cut(s) 333, 410, 575, 626
BstNSI RCATGY 1 cut(s) 595
BstPI GGTNACC 1 cut(s) 307
BstSCI CCNGG 1 cut(s) 506
BstUI CGCG 2 cut(s) 695, 954
BstV1I GCAGC 1 cut(s) 290
BstV2I GAAGAC 2 cut(s) 538, 1140
BstXI CCANNNNNNTGG 1 cut(s) 586
Bsu36I CCTNAGG 1 cut(s) 240
BsuRI GGCC 4 cut(s) 95, 404, 569, 860
BtgI CCRYGG 1 cut(s) 142
BtgZI GCGATG 1 cut(s) 710
BtsCI GGATG 5 cut(s) 11, 354, 570, 1025, 1105
BtsIMutI CAGTG 1 cut(s) 702
Cac8I GCNNGC 1 cut(s) 1051
CciI TCATGA 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 662
CseI GACGC 8 cut(s) 284, 377, 560, 609, 670, 701, 835, 1016
Csp6I GTAC 5 cut(s) 118, 463, 700, 757, 936
CspCI CAANNNNNGTGG 2 cut(s) 595, 630
CviAII CATG 7 cut(s) 284, 331, 586, 592, 657, 833, 1138
CviQI GTAC 5 cut(s) 118, 463, 700, 757, 936
DdeI CTNAG 4 cut(s) 109, 240, 408, 454
DpnI GATC 3 cut(s) 512, 1001, 1118
DpnII GATC 3 cut(s) 510, 999, 1116
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
EciI GGCGGA 1 cut(s) 35
Eco147I AGGCCT 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 1190
Eco81I CCTNAGG 1 cut(s) 240
Eco88I CYCGRG 3 cut(s) 443, 752, 1189
Eco91I GGTNACC 1 cut(s) 307
EcoO65I GGTNACC 1 cut(s) 307
EcoT22I ATGCAT 1 cut(s) 587
Esp3I CGTCTC 1 cut(s) 457
FaeI CATG 7 cut(s) 287, 334, 589, 595, 660, 836, 1141
FaqI GGGAC 3 cut(s) 337, 380, 838
FatI CATG 7 cut(s) 283, 330, 585, 591, 656, 832, 1137
FauI CCCGC 1 cut(s) 1192
FauNDI CATATG 1 cut(s) 635
FblI GTMKAC 1 cut(s) 873
Fnu4HI GCNGC 5 cut(s) 279, 328, 405, 643, 797
FokI GGATG 5 cut(s) 18, 361, 557, 1032, 1112
Fsp4HI GCNGC 5 cut(s) 279, 328, 405, 643, 797
FspBI CTAG 3 cut(s) 128, 272, 1204
GluI GCNGC 5 cut(s) 279, 328, 405, 643, 797
HaeIII GGCC 4 cut(s) 95, 404, 569, 860
HapII CCGG 3 cut(s) 132, 507, 663
HgaI GACGC 8 cut(s) 284, 377, 560, 609, 670, 701, 835, 1016
Hin1I GRCGYC 2 cut(s) 369, 601
Hin1II CATG 7 cut(s) 287, 334, 589, 595, 660, 836, 1141
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HinfI GANTC 2 cut(s) 138, 489
HpaII CCGG 3 cut(s) 132, 507, 663
HphI GGTGA 6 cut(s) 301, 428, 676, 917, 958, 1173
Hpy166II GTNNAC 5 cut(s) 142, 702, 721, 811, 874
Hpy188I TCNGA 6 cut(s) 103, 217, 457, 575, 999, 1170
Hpy188III TCNNGA 8 cut(s) 284, 443, 486, 597, 752, 766, 925, 1063
Hpy8I GTNNAC 5 cut(s) 142, 702, 721, 811, 874
Hpy99I CGWCG 6 cut(s) 161, 173, 191, 554, 695, 1010
HpyAV CCTTC 2 cut(s) 167, 322
HpyCH4III ACNGT 2 cut(s) 706, 718
HpyCH4IV ACGT 7 cut(s) 156, 186, 386, 771, 849, 1058, 1092
HpyCH4V TGCA 5 cut(s) 165, 207, 585, 660, 898
HpyF10VI GCNNNNNNNGC 4 cut(s) 333, 410, 575, 626
HpyF3I CTNAG 4 cut(s) 109, 240, 408, 454
HpySE526I ACGT 7 cut(s) 156, 186, 386, 771, 849, 1058, 1092
Hsp92I GRCGYC 2 cut(s) 369, 601
Hsp92II CATG 7 cut(s) 287, 334, 589, 595, 660, 836, 1141
Kzo9I GATC 3 cut(s) 510, 999, 1116
LmnI GCTCC 6 cut(s) 176, 559, 639, 739, 930, 1162
Lsp1109I GCAGC 1 cut(s) 290
LweI GCATC 1 cut(s) 345
MaeI CTAG 3 cut(s) 128, 272, 1204
MaeII ACGT 7 cut(s) 156, 186, 386, 771, 849, 1058, 1092
MaeIII GTNAC 4 cut(s) 307, 682, 772, 905
MalI GATC 3 cut(s) 512, 1001, 1118
MboI GATC 3 cut(s) 510, 999, 1116
MboII GAAGA 5 cut(s) 147, 307, 484, 538, 1145
MluCI AATT 3 cut(s) 844, 893, 1145
MlyI GAGTC 1 cut(s) 147
MmeI TCCRAC 4 cut(s) 42, 53, 126, 520
Mph1103I ATGCAT 1 cut(s) 587
MseI TTAA 3 cut(s) 843, 864, 1035
MslI CAYNNNNRTG 4 cut(s) 347, 590, 734, 791
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 3 cut(s) 132, 507, 663
MspR9I CCNGG 1 cut(s) 508
Mva1269I GAATGC 2 cut(s) 666, 761
MvnI CGCG 2 cut(s) 695, 954
MwoI GCNNNNNNNGC 4 cut(s) 333, 410, 575, 626
NciI CCSGG 1 cut(s) 508
NdeI CATATG 1 cut(s) 635
NdeII GATC 3 cut(s) 510, 999, 1116
NlaIII CATG 7 cut(s) 287, 334, 589, 595, 660, 836, 1141
NlaIV GGNNCC 6 cut(s) 148, 178, 641, 741, 795, 932
NmuCI GTSAC 4 cut(s) 307, 682, 772, 905
NsiI ATGCAT 1 cut(s) 587
NspI RCATGY 1 cut(s) 595
PagI TCATGA 1 cut(s) 283
PceI AGGCCT 1 cut(s) 95
PciI ACATGT 1 cut(s) 591
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 607, 1155
PctI GAATGC 2 cut(s) 666, 761
PfeI GAWTC 1 cut(s) 489
Pfl23II CGTACG 1 cut(s) 935
PkrI GCNGC 5 cut(s) 280, 329, 406, 644, 798
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PscI ACATGT 1 cut(s) 591
PshBI ATTAAT 1 cut(s) 1035
PspEI GGTNACC 1 cut(s) 307
PspLI CGTACG 1 cut(s) 935
PspN4I GGNNCC 6 cut(s) 148, 178, 641, 741, 795, 932
RsaI GTAC 5 cut(s) 119, 464, 701, 758, 937
RsaNI GTAC 5 cut(s) 118, 463, 700, 757, 936
RseI CAYNNNNRTG 4 cut(s) 347, 590, 734, 791
SaqAI TTAA 3 cut(s) 843, 864, 1035
SatI GCNGC 5 cut(s) 279, 328, 405, 643, 797
Sau3AI GATC 3 cut(s) 510, 999, 1116
ScaI AGTACT 2 cut(s) 119, 464
SchI GAGTC 1 cut(s) 147
ScrFI CCNGG 1 cut(s) 508
SfaNI GCATC 1 cut(s) 345
SmiMI CAYNNNNRTG 4 cut(s) 347, 590, 734, 791
SmlI CTYRAG 2 cut(s) 916, 925
SmoI CTYRAG 2 cut(s) 916, 925
Sse9I AATT 3 cut(s) 844, 893, 1145
SseBI AGGCCT 1 cut(s) 95
SspMI CTAG 3 cut(s) 128, 272, 1204
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 506
TaaI ACNGT 2 cut(s) 706, 718
TaiI ACGT 7 cut(s) 159, 189, 389, 774, 852, 1061, 1095
TaqI TCGA 7 cut(s) 69, 159, 189, 225, 267, 549, 1149
TaqII GACCGA 1 cut(s) 215
TasI AATT 3 cut(s) 844, 893, 1145
TatI WGTACW 3 cut(s) 117, 462, 699
TauI GCSGC 4 cut(s) 330, 407, 645, 799
TfiI GAWTC 1 cut(s) 489
Tru1I TTAA 3 cut(s) 843, 864, 1035
Tru9I TTAA 3 cut(s) 843, 864, 1035
TscAI CASTG 1 cut(s) 709
TseFI GTSAC 4 cut(s) 307, 682, 772, 905
TseI GCWGC 1 cut(s) 278
Tsp45I GTSAC 4 cut(s) 307, 682, 772, 905
TspDTI ATGAA 3 cut(s) 622, 821, 874
TspGWI ACGGA 3 cut(s) 159, 924, 953
TspRI CASTG 1 cut(s) 709
VspI ATTAAT 1 cut(s) 1035
XapI RAATTY 1 cut(s) 1145
XceI RCATGY 1 cut(s) 595
XmiI GTMKAC 1 cut(s) 873
XspI CTAG 3 cut(s) 128, 272, 1204
ZrmI AGTACT 2 cut(s) 119, 464
Zsp2I ATGCAT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.