MD16G1108700.v1.1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000852
Physical Location & Seq
Forward (+)
7614482 .. 7615777
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1108700.v1.1.491

Sequence Viewer

Length: 1296 bp
ATGAAGATTGAAGTGATTGAGAGAGCTTACATTAAGCCTTCTATGCCAACCCCACCCCATCTTTGTAAGCATGAGTTTTCTTTTCTCGACCAAATCGCAACACACATTTTCATGCCTATGATCTTTTTCTACAATAATAATCATGTTAACGATGCAAGAACTTATTCAGATAAGGTAAAACAATCTTTATCTGAGATCTTAACACAATATTATCCGTTGGCTGGACGTATCATTGAAAATGCCTTCATCGATTGCAACGACCAAGGTGCGCCTTACGTCCAAGCCCGAGTGAGGTCCCCACTTTCCGATGTCATATCCGAAGCAGACCCAAACCAGCTCAACAAACTACTCCCATGTGAACTAGACAAAGTCGGTGACTTGGTTCTTGCTATCCAAGTCAGCATGTTCGATTGTGGTGGCATGGCTATTGGAATTTGCATTTCTCACAAAGTTGCCGATGCATTGTCATTCATCACGTTCCTCAACAGTTGGGCGTCGATATCTCGTGGAGATTGCAACATTACAAATAGTACAGTCAGTCCTCCAATTTTTGATTTGGCCACGCTCTTTCCGCCTAGAAGCATATCAGGATTCAAACCAAGCACTGGGATCGCTAGAGAAAAAATCGTCACAAAAAGGTTTGTTTTCAGTGCTTCCACGATTGCTTCTCTTAGGTCAAAATATACAGATAATAAAGCCAATGAGATAAAGCCAAGTCGGATTGAGGCCTTGTCAGCATTTATATGGGCCCGGTTTATAGCCTCCACTCAAGGAAAGCCGAACCCCAACACTCTTTACAGAGTGCTTCACACCGTTAACTTGCGGACGAGGATGGACCCACCACTGCCTGAATACCATTTCGGGAACGTGAGCCGGTTTGTCATGTCCTCGCCGTCGTTTGACGAGGGAGAGGACACGTGTTACGGGATCGTGAGCCAGATGAGGCAGGCTATAAAGACGATCGACGGTGATTACATAGCCAAAATCAAAGAGGGCAACGAGCTCTTGGAGTTTTTGAAGCAGCGAATGGAGGAGTTTATGAAGGGTGAAGTAGCGTCGTTTAGCTTTACCAGCTTGTGCAGGTTCCCACTTTATGAGACGGACTTCGGGTGGGGCAAGCCTGTGTGGGTCGGGTCGGTCAGCTTTCCATTTAAGAACCTGGTTAGTTTCATTGATGCTGGGTCGAGTGGTGGGATTGAAGCATGGGTGAATTTGAAGGAGGAAGACATGGCTAAGTTCCAACATGATGAGCAGCTGCTGGCATGTACTTATCCAGCACAAGATGCCAAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

432

Amino Acids

48.38

Weight (kDa)

6.11

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 418 3.7e-89 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1071
AccB7I CCANNNNNTGG 1 cut(s) 605
AciI CCGC 2 cut(s) 572, 823
AclWI GGATC 2 cut(s) 617, 935
AcoI YGGCCR 1 cut(s) 558
AcsI RAATTY 2 cut(s) 432, 1210
AcvI CACGTG 1 cut(s) 918
AcyI GRCGYC 1 cut(s) 494
AfaI GTAC 2 cut(s) 532, 1267
AfiI CCNNNNNNNGG 3 cut(s) 221, 291, 605
AflIII ACRYGT 2 cut(s) 915, 917
AgsI TTSAA 6 cut(s) 11, 236, 595, 1018, 1199, 1216
AjnI CCWGG 1 cut(s) 1158
AluBI AGCT 7 cut(s) 26, 337, 1003, 1065, 1074, 1143, 1255
AluI AGCT 7 cut(s) 26, 337, 1003, 1065, 1074, 1143, 1255
Alw21I GWGCWC 1 cut(s) 1005
Alw26I GTCTC 1 cut(s) 1091
AlwI GGATC 2 cut(s) 617, 935
AlwNI CAGNNNCTG 1 cut(s) 1258
Ama87I CYCGRG 1 cut(s) 285
AoxI GGCC 3 cut(s) 558, 726, 747
ApaI GGGCCC 1 cut(s) 751
ApeKI GCWGC 3 cut(s) 1021, 1252, 1255
ApoI RAATTY 2 cut(s) 432, 1210
Asp700I GAANNNNTTC 1 cut(s) 163
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 4 cut(s) 294, 747, 748, 835
AsuC2I CCSGG 1 cut(s) 751
AsuHPI GGTGA 4 cut(s) 386, 980, 1058, 1219
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 2 cut(s) 294, 835
BaeGI GKGCMC 1 cut(s) 751
BalI TGGCCA 1 cut(s) 560
BanII GRGCYC 2 cut(s) 751, 1005
BauI CACGAG 1 cut(s) 504
BbrPI CACGTG 1 cut(s) 918
BbsI GAAGAC 1 cut(s) 1230
Bbv12I GWGCWC 1 cut(s) 1005
BbvI GCAGC 3 cut(s) 1033, 1242, 1264
BccI CCATC 2 cut(s) 66, 826
BceAI ACGGC 1 cut(s) 877
BcgI CGANNNNNNTGC 4 cut(s) 248, 282, 592, 626
BciT130I CCWGG 1 cut(s) 1160
BcnI CCSGG 1 cut(s) 751
BcoDI GTCTC 1 cut(s) 1091
BfaI CTAG 3 cut(s) 362, 576, 615
BfuAI ACCTGC 1 cut(s) 1071
BglII AGATCT 1 cut(s) 195
BisI GCNGC 3 cut(s) 1022, 1253, 1256
BlsI GCNGC 3 cut(s) 1023, 1254, 1257
Bme1390I CCNGG 2 cut(s) 751, 1160
Bme18I GGWCC 2 cut(s) 294, 835
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 4 cut(s) 294, 747, 748, 835
BmiI GGNNCC 4 cut(s) 296, 749, 837, 1085
BmrFI CCNGG 2 cut(s) 751, 1160
BmrI ACTGGG 1 cut(s) 615
BmsI GCATC 4 cut(s) 142, 448, 1165, 1273
BmuI ACTGGG 1 cut(s) 615
BpiI GAAGAC 1 cut(s) 1230
BpuEI CTTGAG 1 cut(s) 753
BpuMI CCSGG 1 cut(s) 751
Bsa29I ATCGAT 1 cut(s) 249
BsaAI YACGTR 1 cut(s) 918
BsaHI GRCGYC 1 cut(s) 494
BsaJI CCNNGG 2 cut(s) 262, 1287
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 291, 605
Bse118I RCCGGY 1 cut(s) 873
Bse1I ACTGG 1 cut(s) 610
BseBI CCWGG 1 cut(s) 1160
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 2 cut(s) 262, 1287
BseGI GGATG 1 cut(s) 837
BseLI CCNNNNNNNGG 3 cut(s) 221, 291, 605
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 610
BseRI GAGGAG 1 cut(s) 1046
BseSI GKGCMC 1 cut(s) 751
BseXI GCAGC 3 cut(s) 1033, 1242, 1264
BseYI CCCAGC 1 cut(s) 1178
BsgI GTGCAG 1 cut(s) 1099
Bsh1285I CGRYCG 1 cut(s) 963
BshFI GGCC 3 cut(s) 560, 728, 749
BshVI ATCGAT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 963
BsiHKAI GWGCWC 1 cut(s) 1005
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 2 cut(s) 751, 874
BslFI GGGAC 1 cut(s) 280
BslI CCNNNNNNNGG 3 cut(s) 221, 291, 605
BsmAI GTCTC 1 cut(s) 1091
BsmBI CGTCTC 1 cut(s) 1091
BsmFI GGGAC 1 cut(s) 280
BsnI GGCC 3 cut(s) 560, 728, 749
BsoBI CYCGRG 1 cut(s) 285
Bsp120I GGGCCC 1 cut(s) 747
Bsp1286I GDGCHC 2 cut(s) 751, 1005
Bsp143I GATC 5 cut(s) 120, 195, 609, 927, 960
BspACI CCGC 2 cut(s) 572, 823
BspANI GGCC 3 cut(s) 560, 728, 749
BspCNI CTCAG 1 cut(s) 184
BspDI ATCGAT 1 cut(s) 249
BspLI GGNNCC 4 cut(s) 296, 749, 837, 1085
BspMI ACCTGC 1 cut(s) 1071
BspPI GGATC 2 cut(s) 617, 935
BsrFI RCCGGY 1 cut(s) 873
BsrI ACTGG 1 cut(s) 610
BssAI RCCGGY 1 cut(s) 873
BssECI CCNNGG 2 cut(s) 262, 1287
BssMI GATC 5 cut(s) 120, 195, 609, 927, 960
BssNI GRCGYC 1 cut(s) 494
BssSI CACGAG 1 cut(s) 504
BssT1I CCWWGG 2 cut(s) 262, 1287
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 1 cut(s) 1160
Bst4CI ACNGT 4 cut(s) 488, 535, 814, 968
BstACI GRCGYC 1 cut(s) 494
BstAPI GCANNNNNTGC 1 cut(s) 1283
BstBAI YACGTR 1 cut(s) 918
BstC8I GCNNGC 3 cut(s) 948, 1118, 1260
BstDEI CTNAG 3 cut(s) 192, 671, 1233
BstF5I GGATG 1 cut(s) 837
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 5 cut(s) 123, 198, 612, 930, 963
BstMAI GTCTC 1 cut(s) 1091
BstMBI GATC 5 cut(s) 120, 195, 609, 927, 960
BstMCI CGRYCG 1 cut(s) 963
BstMWI GCNNNNNNNGC 5 cut(s) 43, 571, 734, 1071, 1283
BstNI CCWGG 1 cut(s) 1160
BstNSI RCATGY 2 cut(s) 406, 1266
BstSCI CCNGG 2 cut(s) 749, 1158
BstSLI GKGCMC 1 cut(s) 751
BstV1I GCAGC 3 cut(s) 1033, 1242, 1264
BstV2I GAAGAC 1 cut(s) 1230
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
Bsu15I ATCGAT 1 cut(s) 249
BsuRI GGCC 3 cut(s) 560, 728, 749
BsuTUI ATCGAT 1 cut(s) 249
BtsCI GGATG 1 cut(s) 837
BtsI GCAGTG 1 cut(s) 842
BtsIMutI CAGTG 3 cut(s) 603, 655, 842
BveI ACCTGC 1 cut(s) 1071
Cac8I GCNNGC 3 cut(s) 948, 1118, 1260
CaiI CAGNNNCTG 1 cut(s) 1258
CfoI GCGC 1 cut(s) 271
Cfr10I RCCGGY 1 cut(s) 873
Cfr13I GGNCC 4 cut(s) 294, 747, 748, 835
ClaI ATCGAT 1 cut(s) 249
CseI GACGC 2 cut(s) 483, 1044
CsiI ACCWGGT 1 cut(s) 1158
Csp6I GTAC 2 cut(s) 531, 1266
CviQI GTAC 2 cut(s) 531, 1266
DdeI CTNAG 3 cut(s) 192, 671, 1233
DpnI GATC 5 cut(s) 122, 197, 611, 929, 962
DpnII GATC 5 cut(s) 120, 195, 609, 927, 960
EaeI YGGCCR 1 cut(s) 558
EciI GGCGGA 1 cut(s) 561
Ecl136II GAGCTC 1 cut(s) 1003
Eco130I CCWWGG 2 cut(s) 262, 1287
Eco147I AGGCCT 1 cut(s) 728
Eco24I GRGCYC 2 cut(s) 751, 1005
Eco32I GATATC 1 cut(s) 501
Eco47I GGWCC 2 cut(s) 294, 835
Eco53kI GAGCTC 1 cut(s) 1003
Eco72I CACGTG 1 cut(s) 918
Eco88I CYCGRG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 1003
EcoO109I RGGNCCY 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 1158
EcoRV GATATC 1 cut(s) 501
EcoT14I CCWWGG 2 cut(s) 262, 1287
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 2 cut(s) 751, 1005
ErhI CCWWGG 2 cut(s) 262, 1287
Esp3I CGTCTC 1 cut(s) 1091
FaqI GGGAC 1 cut(s) 280
Fnu4HI GCNGC 3 cut(s) 1022, 1253, 1256
FokI GGATG 1 cut(s) 844
FriOI GRGCYC 2 cut(s) 751, 1005
Fsp4HI GCNGC 3 cut(s) 1022, 1253, 1256
FspBI CTAG 3 cut(s) 362, 576, 615
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 1022, 1253, 1256
GsaI CCCAGC 1 cut(s) 1182
HaeIII GGCC 3 cut(s) 560, 728, 749
HapII CCGG 2 cut(s) 751, 874
HgaI GACGC 2 cut(s) 483, 1044
HhaI GCGC 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 494
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 2 cut(s) 148, 817
HindII GTYRAC 2 cut(s) 148, 817
HinfI GANTC 1 cut(s) 591
HpaI GTTAAC 2 cut(s) 148, 817
HpaII CCGG 2 cut(s) 751, 874
HphI GGTGA 4 cut(s) 386, 980, 1058, 1219
Hpy166II GTNNAC 3 cut(s) 148, 359, 817
Hpy188I TCNGA 5 cut(s) 169, 193, 307, 319, 720
Hpy188III TCNNGA 4 cut(s) 86, 588, 862, 931
Hpy8I GTNNAC 3 cut(s) 148, 359, 817
Hpy99I CGWCG 4 cut(s) 499, 898, 968, 1060
HpyAV CCTTC 4 cut(s) 48, 253, 1036, 1210
HpyCH4III ACNGT 4 cut(s) 488, 535, 814, 968
HpyCH4IV ACGT 5 cut(s) 226, 276, 476, 867, 917
HpyCH4V TGCA 6 cut(s) 155, 255, 438, 461, 516, 1080
HpyF10VI GCNNNNNNNGC 5 cut(s) 43, 571, 734, 1071, 1283
HpyF3I CTNAG 3 cut(s) 192, 671, 1233
HpySE526I ACGT 5 cut(s) 226, 276, 476, 867, 917
Hsp92I GRCGYC 1 cut(s) 494
HspAI GCGC 1 cut(s) 269
KspAI GTTAAC 2 cut(s) 148, 817
Kzo9I GATC 5 cut(s) 120, 195, 609, 927, 960
Lsp1109I GCAGC 3 cut(s) 1033, 1242, 1264
LweI GCATC 4 cut(s) 142, 448, 1165, 1273
MabI ACCWGGT 1 cut(s) 1158
MaeI CTAG 3 cut(s) 362, 576, 615
MaeII ACGT 5 cut(s) 226, 276, 476, 867, 917
MaeIII GTNAC 3 cut(s) 374, 628, 920
MalI GATC 5 cut(s) 122, 197, 611, 929, 962
MboI GATC 5 cut(s) 120, 195, 609, 927, 960
MboII GAAGA 2 cut(s) 16, 1235
MflI RGATCY 1 cut(s) 195
MhlI GDGCHC 2 cut(s) 751, 1005
MlsI TGGCCA 1 cut(s) 560
MluCI AATT 3 cut(s) 432, 546, 1210
MluNI TGGCCA 1 cut(s) 560
MmeI TCCRAC 2 cut(s) 698, 1264
Mox20I TGGCCA 1 cut(s) 560
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 163
MscI TGGCCA 1 cut(s) 560
MseI TTAA 5 cut(s) 33, 147, 200, 816, 1152
MslI CAYNNNNRTG 3 cut(s) 110, 116, 742
Msp20I TGGCCA 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 1255
MspI CCGG 2 cut(s) 751, 874
MspR9I CCNGG 2 cut(s) 751, 1160
MvaI CCWGG 1 cut(s) 1160
MwoI GCNNNNNNNGC 5 cut(s) 43, 571, 734, 1071, 1283
NciI CCSGG 1 cut(s) 751
NdeII GATC 5 cut(s) 120, 195, 609, 927, 960
NlaIV GGNNCC 4 cut(s) 296, 749, 837, 1085
NmuCI GTSAC 2 cut(s) 374, 628
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 2 cut(s) 406, 1266
PceI AGGCCT 1 cut(s) 728
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 163
PfeI GAWTC 1 cut(s) 591
PflFI GACNNNGTC 1 cut(s) 368
PflMI CCANNNNNTGG 1 cut(s) 605
PkrI GCNGC 3 cut(s) 1023, 1254, 1257
Ple19I CGATCG 1 cut(s) 963
PmaCI CACGTG 1 cut(s) 918
PmlI CACGTG 1 cut(s) 918
Ppu21I YACGTR 1 cut(s) 918
PpuMI RGGWCCY 1 cut(s) 294
Psp124BI GAGCTC 1 cut(s) 1005
Psp5II RGGWCCY 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 1158
PspCI CACGTG 1 cut(s) 918
PspFI CCCAGC 1 cut(s) 1178
PspGI CCWGG 1 cut(s) 1158
PspN4I GGNNCC 4 cut(s) 296, 749, 837, 1085
PspOMI GGGCCC 1 cut(s) 747
PspPI GGNCC 4 cut(s) 294, 747, 748, 835
PspPPI RGGWCCY 1 cut(s) 294
PstNI CAGNNNCTG 1 cut(s) 1258
PsuI RGATCY 1 cut(s) 195
PsyI GACNNNGTC 1 cut(s) 368
PvuI CGATCG 1 cut(s) 963
PvuII CAGCTG 1 cut(s) 1255
RsaI GTAC 2 cut(s) 532, 1267
RsaNI GTAC 2 cut(s) 531, 1266
RseI CAYNNNNRTG 3 cut(s) 110, 116, 742
SacI GAGCTC 1 cut(s) 1005
SaqAI TTAA 5 cut(s) 33, 147, 200, 816, 1152
SatI GCNGC 3 cut(s) 1022, 1253, 1256
Sau3AI GATC 5 cut(s) 120, 195, 609, 927, 960
Sau96I GGNCC 4 cut(s) 294, 747, 748, 835
ScrFI CCNGG 2 cut(s) 751, 1160
SduI GDGCHC 2 cut(s) 751, 1005
SexAI ACCWGGT 1 cut(s) 1158
SfaNI GCATC 4 cut(s) 142, 448, 1165, 1273
SinI GGWCC 2 cut(s) 294, 835
SmiMI CAYNNNNRTG 3 cut(s) 110, 116, 742
SmlI CTYRAG 1 cut(s) 768
SmoI CTYRAG 1 cut(s) 768
Sse9I AATT 3 cut(s) 432, 546, 1210
SseBI AGGCCT 1 cut(s) 728
SsiI CCGC 2 cut(s) 572, 823
SspI AATATT 1 cut(s) 209
SspMI CTAG 3 cut(s) 362, 576, 615
SstI GAGCTC 1 cut(s) 1005
StuI AGGCCT 1 cut(s) 728
StyD4I CCNGG 2 cut(s) 749, 1158
StyI CCWWGG 2 cut(s) 262, 1287
TaaI ACNGT 4 cut(s) 488, 535, 814, 968
TaiI ACGT 5 cut(s) 229, 279, 479, 870, 920
TaqI TCGA 6 cut(s) 87, 249, 408, 497, 963, 1184
TaqII GACCGA 1 cut(s) 1126
TasI AATT 3 cut(s) 432, 546, 1210
TatI WGTACW 2 cut(s) 530, 1265
TfiI GAWTC 1 cut(s) 591
Tru1I TTAA 5 cut(s) 33, 147, 200, 816, 1152
Tru9I TTAA 5 cut(s) 33, 147, 200, 816, 1152
TscAI CASTG 3 cut(s) 610, 655, 849
TseFI GTSAC 2 cut(s) 374, 628
TseI GCWGC 3 cut(s) 1021, 1252, 1255
Tsp45I GTSAC 2 cut(s) 374, 628
TspDTI ATGAA 6 cut(s) 17, 100, 235, 460, 1055, 1159
TspGWI ACGGA 2 cut(s) 204, 1115
TspRI CASTG 3 cut(s) 610, 655, 849
Tth111I GACNNNGTC 1 cut(s) 368
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 2 cut(s) 294, 835
XapI RAATTY 2 cut(s) 432, 1210
XceI RCATGY 2 cut(s) 406, 1266
XmnI GAANNNNTTC 1 cut(s) 163
XspI CTAG 3 cut(s) 362, 576, 615
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.