Prupe.1G237100_v2.0.a1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000852
Physical Location & Seq
Reverse (-)
25164161 .. 25165444
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G237100.1

Sequence Viewer

Length: 1284 bp
ATGAGGATCCAAGTTCTTGAGAGAGATTGCATTAAGCCTTCTTCACCAACCCCACCCCATCTCCGTAAACATGAACTTTCTTTTCTTGACCAAATCGCCACCCCAATTTTCATGCCTATGATTCTTTTCTACACCAACAATGTCAAAATATATTCTGATCGGGTTAAGCAATCCTTATCGGAGATCTTGACACAATATTATCCGCTAGCTGGACGTGTTATCGACGATGCCTACGTCGATTGCAACGACCAAGGTGTACATTATGTCCAAGCCCAAGTGAACTGCTTGCTATCTGACGTGATATCCCAACCCGACCCAAACCAACTCAACAAATTACTCCCGTATGACCTAGACAATGTGGGTGACTTGATTCTTGCTGTCCAAGTCAACATATTTGACTGTGGTGGCATGGCAATTGGAATTTGCATTTCTCACAAAATTGTCGATGCCTTCTCAGTCATCACGTTCCTAAACAGCTGGGCCTCAGTATCTCGGGGAAATTGTGATATCAAAAACAGTACAATTAGCCCCCCCCTTTTTGATGTGGCCACCCTCTTCCCACCCAGAAGCATATCTGGGTACAAACCAAGCACTGGGATCATCAGAGAAAACATTGTGACCAAAAGGTTTGTTTTTAGTGCTTCTATGGTTGCTTCTCTTAGGTCAAAGTACACAGAAAATAATTCGAATGATAGATACCCGACCCGCATTGAGGCCCTGTCATCTTTTATATGGGCCCGGTTTATAGCTTCCACCCAAGGAGAACCCAACCCGAAAAAAATTTACAAAGTGCTTCACGCCGTCAACTTACGGACCAGGATGGACCCACCACTCCCCGAGTATCATATTGGAAACGTGAGCCGGTTTGCGATTGCGACGCTGTCGTTTGACGGGAGCAAGGACACGTGTTATGGGCTGGTTGGCCAGATGAGAGAAGCCTTGAAGACGATCGACAGTGATTACCTGAGTACGATGAGAGATGGCGACACACACTTGAGCTTTTTGAAGCAGCGAGTAGCAGAGTTTCTGAAAGGTGAAGTGGTGTCGTTGAACTTTACTAGCTTGTGCAGGTTCCCGCTTTATGAAACGGACTTTGGGTGGGGAAAGCCCGTGTGGGTCGGGTCGGCTAGCCTCCCTTTTAAGAATTTGGTTGTCTTCATGGATACCGCGTCCGGTGGTGGGATTGAAGCATGGGTTAATTTGAAGGAGGAAGACATGGCCAAGTTCCAAGAGGATGAGCAGCTGCTGGCGTGTACTTATCAAGCCCAAGATGCTAAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

428

Amino Acids

47.92

Weight (kDa)

6.02

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1059
AccB7I CCANNNNNTGG 1 cut(s) 593
AccII CGCG 1 cut(s) 1169
AciI CCGC 4 cut(s) 203, 706, 1076, 1167
AclWI GGATC 2 cut(s) 14, 605
AcoI YGGCCR 3 cut(s) 546, 922, 1218
AcsI RAATTY 3 cut(s) 420, 780, 1144
AcvI CACGTG 1 cut(s) 906
AfaI GTAC 6 cut(s) 258, 520, 581, 671, 970, 1255
AfiI CCNNNNNNNGG 4 cut(s) 209, 593, 712, 1179
AflIII ACRYGT 3 cut(s) 214, 903, 905
AgsI TTSAA 5 cut(s) 943, 1006, 1051, 1187, 1204
AjiI CACGTC 2 cut(s) 215, 298
AjnI CCWGG 1 cut(s) 815
AjuI GAANNNNNNNTTGG 2 cut(s) 1077, 1109
AluBI AGCT 6 cut(s) 209, 477, 749, 999, 1062, 1243
AluI AGCT 6 cut(s) 209, 477, 749, 999, 1062, 1243
AlwI GGATC 2 cut(s) 14, 605
AlwNI CAGNNNCTG 1 cut(s) 1246
Ama87I CYCGRG 2 cut(s) 492, 836
AoxI GGCC 6 cut(s) 480, 546, 714, 735, 922, 1218
ApaI GGGCCC 1 cut(s) 739
ApeKI GCWGC 3 cut(s) 1009, 1240, 1243
ApoI RAATTY 3 cut(s) 420, 780, 1144
AspS9I GGNCC 6 cut(s) 480, 715, 735, 736, 813, 823
AsuC2I CCSGG 1 cut(s) 739
AsuHPI GGTGA 3 cut(s) 36, 374, 1046
AsuII TTCGAA 1 cut(s) 686
AsuNHI GCTAGC 2 cut(s) 205, 1127
AvaI CYCGRG 2 cut(s) 492, 836
AvaII GGWCC 2 cut(s) 813, 823
BaeGI GKGCMC 1 cut(s) 739
BalI TGGCCA 3 cut(s) 548, 924, 1220
BamHI GGATCC 1 cut(s) 6
BanII GRGCYC 1 cut(s) 739
BbrPI CACGTG 1 cut(s) 906
BbsI GAAGAC 3 cut(s) 950, 1147, 1218
BbvI GCAGC 3 cut(s) 1021, 1230, 1252
BccI CCATC 3 cut(s) 66, 814, 974
BceAI ACGGC 1 cut(s) 785
BciT130I CCWGG 1 cut(s) 817
BciVI GTATCC 1 cut(s) 1156
BcnI CCSGG 1 cut(s) 739
BfaI CTAG 4 cut(s) 206, 350, 1059, 1128
BfuAI ACCTGC 1 cut(s) 1059
BfuI GTATCC 1 cut(s) 1156
BglII AGATCT 1 cut(s) 183
BisI GCNGC 3 cut(s) 1010, 1241, 1244
BlsI GCNGC 3 cut(s) 1011, 1242, 1245
Bme1390I CCNGG 2 cut(s) 739, 817
Bme18I GGWCC 2 cut(s) 813, 823
BmeT110I CYCGRG 2 cut(s) 492, 836
BmgBI CACGTC 2 cut(s) 215, 298
BmgT120I GGNCC 6 cut(s) 480, 715, 735, 736, 813, 823
BmiI GGNNCC 4 cut(s) 8, 737, 825, 1073
BmrFI CCNGG 2 cut(s) 739, 817
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 3 cut(s) 217, 436, 1261
BmtI GCTAGC 2 cut(s) 209, 1131
BmuI ACTGGG 1 cut(s) 603
BpiI GAAGAC 3 cut(s) 950, 1147, 1218
Bpu14I TTCGAA 1 cut(s) 686
BpuEI CTTGAG 2 cut(s) 38, 1015
BpuMI CCSGG 1 cut(s) 739
BsaAI YACGTR 1 cut(s) 906
BsaJI CCNNGG 2 cut(s) 250, 757
BsaWI WCCGGW 1 cut(s) 1172
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 593, 712, 1179
Bse118I RCCGGY 1 cut(s) 861
Bse1I ACTGG 1 cut(s) 598
BseBI CCWGG 1 cut(s) 817
BseDI CCNNGG 2 cut(s) 250, 757
BseGI GGATG 2 cut(s) 825, 1240
BseLI CCNNNNNNNGG 4 cut(s) 209, 593, 712, 1179
BseMII CTCAG 3 cut(s) 468, 498, 956
BseNI ACTGG 1 cut(s) 598
BseSI GKGCMC 1 cut(s) 739
BseXI GCAGC 3 cut(s) 1021, 1230, 1252
BseYI CCCAGC 1 cut(s) 477
BsgI GTGCAG 1 cut(s) 1087
Bsh1236I CGCG 1 cut(s) 1169
Bsh1285I CGRYCG 1 cut(s) 951
BshFI GGCC 6 cut(s) 482, 548, 716, 737, 924, 1220
BsiEI CGRYCG 1 cut(s) 951
BsiHKCI CYCGRG 2 cut(s) 492, 836
BsiSI CCGG 3 cut(s) 739, 862, 1173
BslI CCNNNNNNNGG 4 cut(s) 209, 593, 712, 1179
BsnI GGCC 6 cut(s) 482, 548, 716, 737, 924, 1220
BsoBI CYCGRG 2 cut(s) 492, 836
Bsp119I TTCGAA 1 cut(s) 686
Bsp120I GGGCCC 1 cut(s) 735
Bsp1286I GDGCHC 1 cut(s) 739
Bsp1407I TGTACA 1 cut(s) 256
Bsp143I GATC 5 cut(s) 6, 157, 183, 597, 948
BspACI CCGC 4 cut(s) 203, 706, 1076, 1167
BspANI GGCC 6 cut(s) 482, 548, 716, 737, 924, 1220
BspCNI CTCAG 3 cut(s) 467, 497, 957
BspFNI CGCG 1 cut(s) 1169
BspLI GGNNCC 4 cut(s) 8, 737, 825, 1073
BspMI ACCTGC 1 cut(s) 1059
BspOI GCTAGC 2 cut(s) 209, 1131
BspPI GGATC 2 cut(s) 14, 605
BspT104I TTCGAA 1 cut(s) 686
BsrFI RCCGGY 1 cut(s) 861
BsrGI TGTACA 1 cut(s) 256
BsrI ACTGG 1 cut(s) 598
BssAI RCCGGY 1 cut(s) 861
BssECI CCNNGG 2 cut(s) 250, 757
BssMI GATC 5 cut(s) 6, 157, 183, 597, 948
BssT1I CCWWGG 2 cut(s) 250, 757
Bst2UI CCWGG 1 cut(s) 817
Bst4CI ACNGT 3 cut(s) 401, 518, 956
Bst6I CTCTTC 1 cut(s) 560
BstAUI TGTACA 1 cut(s) 256
BstBAI YACGTR 1 cut(s) 906
BstBI TTCGAA 1 cut(s) 686
BstC8I GCNNGC 4 cut(s) 207, 287, 1129, 1248
BstDEI CTNAG 5 cut(s) 454, 484, 659, 965, 1275
BstF5I GGATG 2 cut(s) 825, 1240
BstFNI CGCG 1 cut(s) 1169
BstKTI GATC 5 cut(s) 9, 160, 186, 600, 951
BstMBI GATC 5 cut(s) 6, 157, 183, 597, 948
BstMCI CGRYCG 1 cut(s) 951
BstMWI GCNNNNNNNGC 1 cut(s) 1271
BstNI CCWGG 1 cut(s) 817
BstSCI CCNGG 2 cut(s) 737, 815
BstSLI GKGCMC 1 cut(s) 739
BstUI CGCG 1 cut(s) 1169
BstV1I GCAGC 3 cut(s) 1021, 1230, 1252
BstV2I GAAGAC 3 cut(s) 950, 1147, 1218
BstX2I RGATCY 2 cut(s) 6, 183
BstYI RGATCY 2 cut(s) 6, 183
BsuI GTATCC 1 cut(s) 1156
BsuRI GGCC 6 cut(s) 482, 548, 716, 737, 924, 1220
BtrI CACGTC 2 cut(s) 215, 298
BtsCI GGATG 2 cut(s) 825, 1240
BtsIMutI CAGTG 2 cut(s) 591, 961
BveI ACCTGC 1 cut(s) 1059
Cac8I GCNNGC 4 cut(s) 207, 287, 1129, 1248
CaiI CAGNNNCTG 1 cut(s) 1246
Cfr10I RCCGGY 1 cut(s) 861
Cfr13I GGNCC 6 cut(s) 480, 715, 735, 736, 813, 823
CseI GACGC 2 cut(s) 886, 1158
Csp6I GTAC 6 cut(s) 257, 519, 580, 670, 969, 1254
CviAII CATG 6 cut(s) 71, 112, 409, 1159, 1191, 1216
CviQI GTAC 6 cut(s) 257, 519, 580, 670, 969, 1254
DdeI CTNAG 5 cut(s) 454, 484, 659, 965, 1275
DpnI GATC 5 cut(s) 8, 159, 185, 599, 950
DpnII GATC 5 cut(s) 6, 157, 183, 597, 948
EaeI YGGCCR 3 cut(s) 546, 922, 1218
Eam1104I CTCTTC 1 cut(s) 560
EarI CTCTTC 1 cut(s) 560
Eco130I CCWWGG 2 cut(s) 250, 757
Eco24I GRGCYC 1 cut(s) 739
Eco32I GATATC 2 cut(s) 303, 508
Eco47I GGWCC 2 cut(s) 813, 823
Eco72I CACGTG 1 cut(s) 906
Eco88I CYCGRG 2 cut(s) 492, 836
EcoO109I RGGNCCY 1 cut(s) 715
EcoRII CCWGG 1 cut(s) 815
EcoRV GATATC 2 cut(s) 303, 508
EcoT14I CCWWGG 2 cut(s) 250, 757
EcoT38I GRGCYC 1 cut(s) 739
ErhI CCWWGG 2 cut(s) 250, 757
FaeI CATG 6 cut(s) 74, 115, 412, 1162, 1194, 1219
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FatI CATG 6 cut(s) 70, 111, 408, 1158, 1190, 1215
FauI CCCGC 2 cut(s) 713, 1083
Fnu4HI GCNGC 3 cut(s) 1010, 1241, 1244
FokI GGATG 2 cut(s) 832, 1247
FriOI GRGCYC 1 cut(s) 739
Fsp4HI GCNGC 3 cut(s) 1010, 1241, 1244
FspBI CTAG 4 cut(s) 206, 350, 1059, 1128
GluI GCNGC 3 cut(s) 1010, 1241, 1244
GsaI CCCAGC 1 cut(s) 481
HaeIII GGCC 6 cut(s) 482, 548, 716, 737, 924, 1220
HapII CCGG 3 cut(s) 739, 862, 1173
HgaI GACGC 2 cut(s) 886, 1158
Hin1II CATG 6 cut(s) 74, 115, 412, 1162, 1194, 1219
HincII GTYRAC 2 cut(s) 388, 805
HindII GTYRAC 2 cut(s) 388, 805
HinfI GANTC 2 cut(s) 121, 370
HpaII CCGG 3 cut(s) 739, 862, 1173
HphI GGTGA 3 cut(s) 36, 374, 1046
Hpy166II GTNNAC 7 cut(s) 68, 257, 280, 388, 672, 805, 1254
Hpy188I TCNGA 5 cut(s) 157, 181, 295, 605, 1029
Hpy188III TCNNGA 3 cut(s) 17, 86, 187
Hpy8I GTNNAC 7 cut(s) 68, 257, 280, 388, 672, 805, 1254
Hpy99I CGWCG 3 cut(s) 227, 239, 880
HpyAV CCTTC 3 cut(s) 48, 460, 1198
HpyCH4III ACNGT 3 cut(s) 401, 518, 956
HpyCH4IV ACGT 6 cut(s) 214, 234, 297, 464, 855, 905
HpyCH4V TGCA 4 cut(s) 30, 243, 426, 1068
HpyF10VI GCNNNNNNNGC 1 cut(s) 1271
HpyF3I CTNAG 5 cut(s) 454, 484, 659, 965, 1275
HpySE526I ACGT 6 cut(s) 214, 234, 297, 464, 855, 905
Hsp92II CATG 6 cut(s) 74, 115, 412, 1162, 1194, 1219
Kzo9I GATC 5 cut(s) 6, 157, 183, 597, 948
LmnI GCTCC 1 cut(s) 894
Lsp1109I GCAGC 3 cut(s) 1021, 1230, 1252
LweI GCATC 3 cut(s) 217, 436, 1261
MaeI CTAG 4 cut(s) 206, 350, 1059, 1128
MaeII ACGT 6 cut(s) 214, 234, 297, 464, 855, 905
MaeIII GTNAC 2 cut(s) 362, 616
MalI GATC 5 cut(s) 8, 159, 185, 599, 950
MboI GATC 5 cut(s) 6, 157, 183, 597, 948
MboII GAAGA 5 cut(s) 33, 547, 955, 1147, 1223
MfeI CAATTG 1 cut(s) 414
MflI RGATCY 2 cut(s) 6, 183
MhlI GDGCHC 1 cut(s) 739
MlsI TGGCCA 3 cut(s) 548, 924, 1220
MluNI TGGCCA 3 cut(s) 548, 924, 1220
MnlI CCTC 6 cut(s) 493, 563, 706, 1142, 1201, 1225
Mox20I TGGCCA 3 cut(s) 548, 924, 1220
MscI TGGCCA 3 cut(s) 548, 924, 1220
MseI TTAA 4 cut(s) 33, 165, 1140, 1197
MslI CAYNNNNRTG 1 cut(s) 116
Msp20I TGGCCA 3 cut(s) 548, 924, 1220
MspA1I CMGCKG 2 cut(s) 477, 1243
MspI CCGG 3 cut(s) 739, 862, 1173
MspR9I CCNGG 2 cut(s) 739, 817
MunI CAATTG 1 cut(s) 414
MvaI CCWGG 1 cut(s) 817
MvnI CGCG 1 cut(s) 1169
MwoI GCNNNNNNNGC 1 cut(s) 1271
NciI CCSGG 1 cut(s) 739
NdeII GATC 5 cut(s) 6, 157, 183, 597, 948
NheI GCTAGC 2 cut(s) 205, 1127
NlaIII CATG 6 cut(s) 74, 115, 412, 1162, 1194, 1219
NlaIV GGNNCC 4 cut(s) 8, 737, 825, 1073
NmuCI GTSAC 2 cut(s) 362, 616
NspV TTCGAA 1 cut(s) 686
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 243
PfeI GAWTC 2 cut(s) 121, 370
PflFI GACNNNGTC 1 cut(s) 880
PflMI CCANNNNNTGG 1 cut(s) 593
PkrI GCNGC 3 cut(s) 1011, 1242, 1245
Ple19I CGATCG 1 cut(s) 951
PmaCI CACGTG 1 cut(s) 906
PmlI CACGTG 1 cut(s) 906
Ppu21I YACGTR 1 cut(s) 906
Psp6I CCWGG 1 cut(s) 815
PspCI CACGTG 1 cut(s) 906
PspFI CCCAGC 1 cut(s) 477
PspGI CCWGG 1 cut(s) 815
PspN4I GGNNCC 4 cut(s) 8, 737, 825, 1073
PspOMI GGGCCC 1 cut(s) 735
PspPI GGNCC 6 cut(s) 480, 715, 735, 736, 813, 823
PstNI CAGNNNCTG 1 cut(s) 1246
PsuI RGATCY 2 cut(s) 6, 183
PsyI GACNNNGTC 1 cut(s) 880
PvuI CGATCG 1 cut(s) 951
PvuII CAGCTG 2 cut(s) 477, 1243
RsaI GTAC 6 cut(s) 258, 520, 581, 671, 970, 1255
RsaNI GTAC 6 cut(s) 257, 519, 580, 670, 969, 1254
RseI CAYNNNNRTG 1 cut(s) 116
SaqAI TTAA 4 cut(s) 33, 165, 1140, 1197
SatI GCNGC 3 cut(s) 1010, 1241, 1244
Sau3AI GATC 5 cut(s) 6, 157, 183, 597, 948
Sau96I GGNCC 6 cut(s) 480, 715, 735, 736, 813, 823
ScrFI CCNGG 2 cut(s) 739, 817
SduI GDGCHC 1 cut(s) 739
SfaNI GCATC 3 cut(s) 217, 436, 1261
SfuI TTCGAA 1 cut(s) 686
SinI GGWCC 2 cut(s) 813, 823
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 2 cut(s) 17, 994
SmoI CTYRAG 2 cut(s) 17, 994
SsiI CCGC 4 cut(s) 203, 706, 1076, 1167
SspI AATATT 1 cut(s) 197
SspMI CTAG 4 cut(s) 206, 350, 1059, 1128
StyD4I CCNGG 2 cut(s) 737, 815
StyI CCWWGG 2 cut(s) 250, 757
TaaI ACNGT 3 cut(s) 401, 518, 956
TaiI ACGT 6 cut(s) 217, 237, 300, 467, 858, 908
TaqI TCGA 5 cut(s) 222, 237, 444, 686, 951
TatI WGTACW 4 cut(s) 256, 518, 669, 1253
TfiI GAWTC 2 cut(s) 121, 370
Tru1I TTAA 4 cut(s) 33, 165, 1140, 1197
Tru9I TTAA 4 cut(s) 33, 165, 1140, 1197
TscAI CASTG 2 cut(s) 598, 961
TseFI GTSAC 2 cut(s) 362, 616
TseI GCWGC 3 cut(s) 1009, 1240, 1243
Tsp45I GTSAC 2 cut(s) 362, 616
TspDTI ATGAA 4 cut(s) 87, 100, 1098, 1147
TspGWI ACGGA 3 cut(s) 53, 826, 1103
TspRI CASTG 2 cut(s) 598, 961
Tth111I GACNNNGTC 1 cut(s) 880
Van91I CCANNNNNTGG 1 cut(s) 593
VpaK11BI GGWCC 2 cut(s) 813, 823
XapI RAATTY 3 cut(s) 420, 780, 1144
XspI CTAG 4 cut(s) 206, 350, 1059, 1128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.