Rroxscaffold_5G00373200

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000852
Physical Location & Seq
Reverse (-)
54451937 .. 54463889
11953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373200.1

Sequence Viewer

Length: 2406 bp
ATGGGAAACCCACTAGAAAAGCCCACAAAGCCCACTTCTATACATCCTTTAAAATTTCTTCCATGTGGAAGTCCCACAAGTTCCAACAAGAAGTTGGTCCTAGGGGTCCAGCTCAGCATCTTTGAATGTGGAGGATTTGCAATTGGTCAATGCATCTCTCATAGGCTTGCCGATGGGTCATCTTATTTCATGTTCAGCAAAACTTGGGCGGCCATAGCCCATGGCGAATCCAACATAGAGCATCCAGACTTTCTGTCAGCAACACGTTTTCCACTGAAGGATGTTATGGCATATGATCCAAGCACTGAAATCTCCAGGAATAAAGTCACAAGGAGGTTTTTATTCAGTGCCTCTATGATAGAGCCTCTCAGAGCAAAGTATGGTGGGGAAAGCGCTGTAGGCTTACAAGAAAACCAGAAACGCCCATCACGTGTTGATGCTTTAACGGCTTTCATATGGAGTCGATATATGGCCAATACCAATAAGATTACAGGACCTCATGATGAGAAGTTGTATAGGATTGCCCATGCTGTGAGCCTACGCCCAAGGTTTGATCCACCATTCCCGCAACATTCTTTTGGAAACATTTACCGTGCTGCCACGTCAGGTCCCTTACTAAGTACTGGGGATGCATGCAGTGCATATGGTCGGGCTATGAGGGAAGTACGAGAAGAAATAAACAAGATCGACAATGACTGCGTCAAAGGACTACAGCAGGGTGCTGGGCAGTCGGGGCTCCCCTTCAATGAGAATACTGGTAGACTAACCGAACTAGAGCTGGTTACGTTGGGCTTCAGTAGCTATTGCAGGTTTCCAGTGTATGATAATGATTTCGGTTGGGGAAGGCCTGCGTGGGTGTTCTCAACACCATTGACCTTCAAGAACGTAGCAACTTTCATGGACACCAAAGAGGGTGATGGAATAGAGGTATACATCAGTTTGGAGGAGCAACTCATGGCTAAATTTGAAACTGATGCCGAGTTCTTGGCCCCTATGTGTCTCCAAGTGGGCGCTGAAGAGCCCTTTGCACAACCAGACATACCCATTGTTTGTAGGAGATCAATGATCATGAAGGTTGAGGTTGAGGTTGTCTCCAGAGAGCTTATCAGACCATCTTCTCCAACCCCTGACCACCTCCACAGATACCAACTCTCCTTTCTTGACCAGATATCTCCCCCGGTCTTTAGTCCTTTGGTTCTATTTTATTCATCATCCGAGGCTGATACAAATTTCGACATTGCTGATATATCGAATCGGCTAAAGAAATCCCTCTCCGATGTCTTGGTCCAATTCTACCCACTCGCTGGACGAGCAAATGACAACATGTTCATAGACTGCAACGACAAGGGCATACCCTTCTTGGAAACTCGAGTTAACTGCCAACTATCTGATGTTATCCAAAACCCAATTCCCAGTGAACTCAACAAGTTGGTTCCATTTCAACTAGACGAAGTTGCTGATCTAGCCCTTGGTGTCCAACTAAATGTCTTTGACTGTGGTGGAGTTGCAATTGGTGCTTGCATTTCTCACAAGATAGCAGATGCTTTATCGTTTTTCCTGTTCATCAAAACTTGGGCTGCCACTGCTAGAAAAGATCATTATCATCAGACAGATGATCATACAGTTATGTCTCCACAGTTTGTATCAGCCACACTGTTCCCACCAAGGAACATATCCGGGTTTAATCCACGTGTTGGGATCACAAAAGAGAATATCGTAACAAAGAGGTTTGTGTTTGATGCTTCCAAGATAGATTCTCTTAAATCAAAATATGGTGTTAATGATAATGATCATGAGCATGAGCAGAGCCTAGAAAGTTACCAAAGACCGCCATCTCGTGTGGAGGCTCTATCCGCTTTCATATGGGGTCGCTATGTGGCTGCAACCAAAGTTGGGTCTGAAGCACCTGAGAGGGTCTGTGCCATTGTTCATGCTGTGAACCTCAGGACAAGGTTTGATCCACCACTGCCAGAATGCTCTTTCGGAAATCTCTACCGAATTGCAGTTACGGTTCCACGCCTGGATACAGGGGAGGAGTGCTATGGTCTTGTGAGGCAGGTCAGAGAGCAAGTGAGTCATATTGACAAGGATTATGTGAAGAAATTGCAGGAGGGCAGTGAACACTTGAGTTTCATCAAACAAAGCTCTGAGAATTTCTACAAGGGAGAGATGGTTACACTGAGCTTCACTAGTTTGTGCAGATTTCCTCTGTATGAAGCTGATTTTGGATGGGGAAAGCCTACTTGGGTCGGCTCTCCTGCCCTGACTTTCAATAATTTGGTTGTTTTTATAGACACTAAATCTGGGGGTGGAATAGAAGCATACATTAACATGAAGGAAGGAGATTTGGCTAAAATTGAATCTGACAAAGAGTTCATTTCATTTGTTTCAAGTGAGTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

801

Amino Acids

89.46

Weight (kDa)

5.91

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 31 - 328 3.4e-41 Transferase family
Transferase PF02458 360 - 792 8.6e-79 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 798, 2047
AccB7I CCANNNNNTGG 2 cut(s) 1304, 1694
AccI GTMKAC 2 cut(s) 760, 930
AciI CCGC 4 cut(s) 209, 566, 1829, 1854
AclWI GGATC 4 cut(s) 290, 548, 1706, 1952
AcoI YGGCCR 2 cut(s) 210, 471
AcsI RAATTY 4 cut(s) 53, 962, 1228, 2152
AcuI CTGAAG 4 cut(s) 296, 778, 1035, 1920
AcvI CACGTG 2 cut(s) 431, 1691
AfaI GTAC 2 cut(s) 622, 666
AfeI AGCGCT 1 cut(s) 394
AfiI CCNNNNNNNGG 3 cut(s) 1304, 1694, 1893
AflIII ACRYGT 4 cut(s) 263, 430, 1323, 1690
AgsI TTSAA 8 cut(s) 125, 745, 880, 968, 1442, 2272, 2360, 2391
AhdI GACNNNNNGTC 1 cut(s) 253
AhlI ACTAGT 1 cut(s) 2189
AjiI CACGTC 1 cut(s) 603
AjnI CCWGG 2 cut(s) 314, 2019
AjuI GAANNNNNNNTTGG 8 cut(s) 1140, 1172, 1392, 1424, 2208, 2227, 2240, 2259
AluBI AGCT 7 cut(s) 112, 778, 801, 1102, 2145, 2184, 2219
AluI AGCT 7 cut(s) 112, 778, 801, 1102, 2145, 2184, 2219
Alw26I GTCTC 3 cut(s) 1004, 1096, 1635
AlwI GGATC 4 cut(s) 290, 548, 1706, 1952
Ama87I CYCGRG 1 cut(s) 1368
Aor51HI AGCGCT 1 cut(s) 394
AoxI GGCC 4 cut(s) 210, 471, 845, 987
ApeKI GCWGC 3 cut(s) 596, 1577, 1880
ApoI RAATTY 4 cut(s) 53, 962, 1228, 2152
AspA2I CCTAGG 1 cut(s) 100
AspLEI GCGC 2 cut(s) 395, 1013
AspS9I GGNCC 6 cut(s) 97, 106, 494, 608, 988, 1285
AsuC2I CCSGG 2 cut(s) 1178, 1678
AsuHPI GGTGA 1 cut(s) 926
AvaI CYCGRG 1 cut(s) 1368
AvaII GGWCC 5 cut(s) 97, 106, 494, 608, 1285
AvrII CCTAGG 1 cut(s) 100
AxyI CCTNAGG 1 cut(s) 1943
BalI TGGCCA 1 cut(s) 473
BanII GRGCYC 2 cut(s) 738, 1023
BauI CACGAG 1 cut(s) 1836
BbrPI CACGTG 2 cut(s) 431, 1691
BbvI GCAGC 3 cut(s) 583, 1564, 1867
BccI CCATC 7 cut(s) 167, 433, 911, 1120, 1840, 2164, 2223
BceAI ACGGC 1 cut(s) 462
BcgI CGANNNNNNTGC 2 cut(s) 1359, 1393
BciT130I CCWGG 2 cut(s) 316, 2021
BciVI GTATCC 1 cut(s) 2017
BclI TGATCA 3 cut(s) 1065, 1615, 1789
BcnI CCSGG 2 cut(s) 1178, 1678
BcoDI GTCTC 3 cut(s) 1004, 1096, 1635
BcuI ACTAGT 1 cut(s) 2189
BfaI CTAG 8 cut(s) 14, 101, 773, 1445, 1463, 1587, 1811, 2190
BfmI CTRYAG 2 cut(s) 396, 710
BfoI RGCGCY 2 cut(s) 396, 1014
BfuAI ACCTGC 2 cut(s) 798, 2047
BfuI GTATCC 1 cut(s) 2017
BisI GCNGC 4 cut(s) 210, 597, 1578, 1881
BlnI CCTAGG 1 cut(s) 100
BlpI GCTNAGC 1 cut(s) 113
BlsI GCNGC 4 cut(s) 211, 598, 1579, 1882
BmcAI AGTACT 1 cut(s) 622
Bme1390I CCNGG 4 cut(s) 316, 1178, 1678, 2021
Bme18I GGWCC 5 cut(s) 97, 106, 494, 608, 1285
BmeRI GACNNNNNGTC 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 1368
BmgBI CACGTC 1 cut(s) 603
BmgT120I GGNCC 6 cut(s) 97, 106, 494, 608, 988, 1285
BmiI GGNNCC 6 cut(s) 107, 610, 737, 990, 1434, 2013
BmrFI CCNGG 4 cut(s) 316, 1178, 1678, 2021
BmrI ACTGGG 2 cut(s) 633, 1407
BmsI GCATC 8 cut(s) 126, 162, 250, 427, 619, 964, 1531, 1729
BmuI ACTGGG 2 cut(s) 633, 1407
BplI GAGNNNNNCTC 2 cut(s) 1076, 1108
BpmI CTGGAG 2 cut(s) 298, 1078
Bpu1102I GCTNAGC 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 2146
BpuMI CCSGG 2 cut(s) 1178, 1678
BsaAI YACGTR 2 cut(s) 431, 1691
BsaBI GATNNNNATC 2 cut(s) 1599, 1788
BsaJI CCNNGG 7 cut(s) 100, 220, 545, 1176, 1215, 1468, 1664
BsaXI ACNNNNNCTCC 4 cut(s) 1136, 1166, 2157, 2187
Bsc4I CCNNNNNNNGG 3 cut(s) 1304, 1694, 1893
Bse1I ACTGG 4 cut(s) 628, 760, 815, 1413
Bse21I CCTNAGG 1 cut(s) 1943
Bse3DI GCAATG 1 cut(s) 1236
Bse8I GATNNNNATC 2 cut(s) 1599, 1788
BseBI CCWGG 2 cut(s) 316, 2021
BseDI CCNNGG 7 cut(s) 100, 220, 545, 1176, 1215, 1468, 1664
BseGI GGATG 6 cut(s) 43, 241, 286, 634, 1211, 2234
BseJI GATNNNNATC 2 cut(s) 1599, 1788
BseLI CCNNNNNNNGG 3 cut(s) 1304, 1694, 1893
BseMI GCAATG 1 cut(s) 1236
BseMII CTCAG 6 cut(s) 127, 382, 1899, 1957, 2139, 2171
BseNI ACTGG 4 cut(s) 628, 760, 815, 1413
BseRI GAGGAG 2 cut(s) 959, 2048
BseXI GCAGC 3 cut(s) 583, 1564, 1867
BseYI CCCAGC 1 cut(s) 722
BsgI GTGCAG 1 cut(s) 2218
BshFI GGCC 4 cut(s) 212, 473, 847, 989
BsiHKCI CYCGRG 1 cut(s) 1368
BsiSI CCGG 2 cut(s) 1178, 1677
BslFI GGGAC 2 cut(s) 57, 594
BslI CCNNNNNNNGG 3 cut(s) 1304, 1694, 1893
BsmAI GTCTC 3 cut(s) 1004, 1096, 1635
BsmFI GGGAC 2 cut(s) 57, 594
BsmI GAATGC 1 cut(s) 1979
BsnI GGCC 4 cut(s) 212, 473, 847, 989
BsoBI CYCGRG 1 cut(s) 1368
Bsp1286I GDGCHC 2 cut(s) 738, 1023
Bsp1720I GCTNAGC 1 cut(s) 113
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 4 cut(s) 209, 566, 1829, 1854
BspANI GGCC 4 cut(s) 212, 473, 847, 989
BspCNI CTCAG 6 cut(s) 126, 381, 1900, 1956, 2140, 2172
BspHI TCATGA 3 cut(s) 499, 1068, 1792
BspLI GGNNCC 6 cut(s) 107, 610, 737, 990, 1434, 2013
BspMI ACCTGC 2 cut(s) 798, 2047
BspPI GGATC 4 cut(s) 290, 548, 1706, 1952
BspQI GCTCTTC 1 cut(s) 1011
BsrDI GCAATG 1 cut(s) 1236
BsrI ACTGG 4 cut(s) 628, 760, 815, 1413
BssECI CCNNGG 7 cut(s) 100, 220, 545, 1176, 1215, 1468, 1664
BssNAI GTATAC 1 cut(s) 931
BssSI CACGAG 1 cut(s) 1836
BssT1I CCWWGG 5 cut(s) 100, 220, 545, 1468, 1664
Bst1107I GTATAC 1 cut(s) 931
Bst2BI CACGAG 1 cut(s) 1836
Bst2UI CCWGG 2 cut(s) 316, 2021
Bst4CI ACNGT 6 cut(s) 593, 1496, 1624, 1638, 1656, 2011
Bst6I CTCTTC 1 cut(s) 1011
BstAPI GCANNNNNTGC 2 cut(s) 638, 1514
BstBAI YACGTR 2 cut(s) 431, 1691
BstC8I GCNNGC 4 cut(s) 168, 634, 849, 1519
BstDEI CTNAG 7 cut(s) 113, 368, 617, 1908, 1943, 2148, 2180
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 6 cut(s) 43, 241, 286, 634, 1211, 2234
BstH2I RGCGCY 2 cut(s) 396, 1014
BstHHI GCGC 2 cut(s) 395, 1013
BstMAI GTCTC 3 cut(s) 1004, 1096, 1635
BstNI CCWGG 2 cut(s) 316, 2021
BstNSI RCATGY 2 cut(s) 636, 1327
BstSCI CCNGG 4 cut(s) 314, 1176, 1676, 2019
BstSFI CTRYAG 2 cut(s) 396, 710
BstV1I GCAGC 3 cut(s) 583, 1564, 1867
BstZ17I GTATAC 1 cut(s) 931
Bsu36I CCTNAGG 1 cut(s) 1943
BsuI GTATCC 1 cut(s) 2017
BsuRI GGCC 4 cut(s) 212, 473, 847, 989
BtgI CCRYGG 1 cut(s) 220
BtrI CACGTC 1 cut(s) 603
BtsCI GGATG 6 cut(s) 43, 241, 286, 634, 1211, 2234
BtsI GCAGTG 4 cut(s) 643, 1581, 1964, 2122
BveI ACCTGC 2 cut(s) 798, 2047
Cac8I GCNNGC 4 cut(s) 168, 634, 849, 1519
CciI TCATGA 3 cut(s) 499, 1068, 1792
CfoI GCGC 2 cut(s) 395, 1013
Cfr13I GGNCC 6 cut(s) 97, 106, 494, 608, 988, 1285
CseI GACGC 1 cut(s) 688
Csp6I GTAC 2 cut(s) 621, 665
CviQI GTAC 2 cut(s) 621, 665
DdeI CTNAG 7 cut(s) 113, 368, 617, 1908, 1943, 2148, 2180
DraI TTTAAA 1 cut(s) 51
DriI GACNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 2 cut(s) 210, 471
Eam1104I CTCTTC 1 cut(s) 1011
Eam1105I GACNNNNNGTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 1011
Eco130I CCWWGG 5 cut(s) 100, 220, 545, 1468, 1664
Eco147I AGGCCT 1 cut(s) 847
Eco24I GRGCYC 2 cut(s) 738, 1023
Eco32I GATATC 1 cut(s) 1170
Eco47I GGWCC 5 cut(s) 97, 106, 494, 608, 1285
Eco47III AGCGCT 1 cut(s) 394
Eco57I CTGAAG 4 cut(s) 296, 778, 1035, 1920
Eco72I CACGTG 2 cut(s) 431, 1691
Eco81I CCTNAGG 1 cut(s) 1943
Eco88I CYCGRG 1 cut(s) 1368
EcoO109I RGGNCCY 2 cut(s) 494, 608
EcoRII CCWGG 2 cut(s) 314, 2019
EcoRV GATATC 1 cut(s) 1170
EcoT14I CCWWGG 5 cut(s) 100, 220, 545, 1468, 1664
EcoT22I ATGCAT 2 cut(s) 155, 634
EcoT38I GRGCYC 2 cut(s) 738, 1023
ErhI CCWWGG 5 cut(s) 100, 220, 545, 1468, 1664
FaqI GGGAC 2 cut(s) 57, 594
FauI CCCGC 1 cut(s) 573
FauNDI CATATG 4 cut(s) 292, 455, 643, 1862
FbaI TGATCA 3 cut(s) 1065, 1615, 1789
FblI GTMKAC 2 cut(s) 760, 930
Fnu4HI GCNGC 4 cut(s) 210, 597, 1578, 1881
FokI GGATG 6 cut(s) 30, 228, 293, 641, 1198, 2241
FriOI GRGCYC 2 cut(s) 738, 1023
Fsp4HI GCNGC 4 cut(s) 210, 597, 1578, 1881
FspBI CTAG 8 cut(s) 14, 101, 773, 1445, 1463, 1587, 1811, 2190
GlaI GCGC 2 cut(s) 394, 1012
GluI GCNGC 4 cut(s) 210, 597, 1578, 1881
GsaI CCCAGC 1 cut(s) 726
GsuI CTGGAG 2 cut(s) 298, 1078
HaeII RGCGCY 2 cut(s) 396, 1014
HaeIII GGCC 4 cut(s) 212, 473, 847, 989
HapII CCGG 2 cut(s) 1178, 1677
HgaI GACGC 1 cut(s) 688
HhaI GCGC 2 cut(s) 395, 1013
Hin6I GCGC 2 cut(s) 393, 1011
HinP1I GCGC 2 cut(s) 393, 1011
HincII GTYRAC 1 cut(s) 1375
HindII GTYRAC 1 cut(s) 1375
HinfI GANTC 7 cut(s) 227, 460, 1252, 1754, 2074, 2360, 2396
HpaI GTTAAC 1 cut(s) 1375
HpaII CCGG 2 cut(s) 1178, 1677
HphI GGTGA 1 cut(s) 926
Hpy166II GTNNAC 6 cut(s) 761, 931, 1375, 1418, 1939, 2120
Hpy188III TCNNGA 8 cut(s) 245, 500, 880, 1069, 1095, 1160, 1793, 1945
Hpy8I GTNNAC 6 cut(s) 761, 931, 1375, 1418, 1939, 2120
HpyAV CCTTC 8 cut(s) 271, 751, 837, 886, 1066, 1366, 2329, 2333
HpyCH4III ACNGT 6 cut(s) 593, 1496, 1624, 1638, 1656, 2011
HpyCH4IV ACGT 6 cut(s) 265, 430, 602, 785, 885, 1690
HpyF3I CTNAG 7 cut(s) 113, 368, 617, 1908, 1943, 2148, 2180
HpySE526I ACGT 6 cut(s) 265, 430, 602, 785, 885, 1690
HspAI GCGC 2 cut(s) 393, 1011
Ksp22I TGATCA 3 cut(s) 1065, 1615, 1789
KspAI GTTAAC 1 cut(s) 1375
LguI GCTCTTC 1 cut(s) 1011
LmnI GCTCC 2 cut(s) 741, 946
Lsp1109I GCAGC 3 cut(s) 583, 1564, 1867
LweI GCATC 8 cut(s) 126, 162, 250, 427, 619, 964, 1531, 1729
MaeI CTAG 8 cut(s) 14, 101, 773, 1445, 1463, 1587, 1811, 2190
MaeII ACGT 6 cut(s) 265, 430, 602, 785, 885, 1690
MaeIII GTNAC 6 cut(s) 325, 781, 1717, 1817, 2005, 2173
MboII GAAGA 5 cut(s) 50, 683, 1028, 1107, 2110
MfeI CAATTG 2 cut(s) 141, 1509
MhlI GDGCHC 2 cut(s) 738, 1023
MlsI TGGCCA 1 cut(s) 473
MluNI TGGCCA 1 cut(s) 473
MlyI GAGTC 3 cut(s) 469, 2083, 2405
MmeI TCCRAC 4 cut(s) 108, 255, 1145, 1501
Mox20I TGGCCA 1 cut(s) 473
Mph1103I ATGCAT 2 cut(s) 155, 634
MscI TGGCCA 1 cut(s) 473
MseI TTAA 7 cut(s) 50, 443, 1374, 1683, 1761, 1779, 2328
MslI CAYNNNNRTG 2 cut(s) 1797, 2330
Msp20I TGGCCA 1 cut(s) 473
MspI CCGG 2 cut(s) 1178, 1677
MspR9I CCNGG 4 cut(s) 316, 1178, 1678, 2021
MunI CAATTG 2 cut(s) 141, 1509
Mva1269I GAATGC 1 cut(s) 1979
MvaI CCWGG 2 cut(s) 316, 2021
NciI CCSGG 2 cut(s) 1178, 1678
NcoI CCATGG 1 cut(s) 220
NdeI CATATG 4 cut(s) 292, 455, 643, 1862
NlaIV GGNNCC 6 cut(s) 107, 610, 737, 990, 1434, 2013
NmeAIII GCCGAG 1 cut(s) 1003
NmuCI GTSAC 1 cut(s) 325
NsiI ATGCAT 2 cut(s) 155, 634
NspI RCATGY 2 cut(s) 636, 1327
PaeI GCATGC 1 cut(s) 636
PaeR7I CTCGAG 1 cut(s) 1368
PagI TCATGA 3 cut(s) 499, 1068, 1792
PceI AGGCCT 1 cut(s) 847
PciI ACATGT 1 cut(s) 1323
PciSI GCTCTTC 1 cut(s) 1011
PcsI WCGNNNNNNNCGW 1 cut(s) 427
PctI GAATGC 1 cut(s) 1979
PfeI GAWTC 4 cut(s) 227, 1252, 1754, 2360
PflFI GACNNNGTC 1 cut(s) 698
PflMI CCANNNNNTGG 2 cut(s) 1304, 1694
PfoI TCCNGGA 1 cut(s) 314
PkrI GCNGC 4 cut(s) 211, 598, 1579, 1882
PleI GAGTC 3 cut(s) 468, 2082, 2404
PmaCI CACGTG 2 cut(s) 431, 1691
PmlI CACGTG 2 cut(s) 431, 1691
PpsI GAGTC 3 cut(s) 468, 2082, 2404
Ppu21I YACGTR 2 cut(s) 431, 1691
PpuMI RGGWCCY 2 cut(s) 494, 608
PscI ACATGT 1 cut(s) 1323
Psp5II RGGWCCY 2 cut(s) 494, 608
Psp6I CCWGG 2 cut(s) 314, 2019
PspCI CACGTG 2 cut(s) 431, 1691
PspFI CCCAGC 1 cut(s) 722
PspGI CCWGG 2 cut(s) 314, 2019
PspN4I GGNNCC 6 cut(s) 107, 610, 737, 990, 1434, 2013
PspPI GGNCC 6 cut(s) 97, 106, 494, 608, 988, 1285
PspPPI RGGWCCY 2 cut(s) 494, 608
PspXI VCTCGAGB 1 cut(s) 1368
PsyI GACNNNGTC 1 cut(s) 698
RsaI GTAC 2 cut(s) 622, 666
RsaNI GTAC 2 cut(s) 621, 665
RseI CAYNNNNRTG 2 cut(s) 1797, 2330
SapI GCTCTTC 1 cut(s) 1011
SaqAI TTAA 7 cut(s) 50, 443, 1374, 1683, 1761, 1779, 2328
SatI GCNGC 4 cut(s) 210, 597, 1578, 1881
Sau96I GGNCC 6 cut(s) 97, 106, 494, 608, 988, 1285
ScaI AGTACT 1 cut(s) 622
SchI GAGTC 3 cut(s) 469, 2083, 2405
ScrFI CCNGG 4 cut(s) 316, 1178, 1678, 2021
SduI GDGCHC 2 cut(s) 738, 1023
SfaNI GCATC 8 cut(s) 126, 162, 250, 427, 619, 964, 1531, 1729
SfcI CTRYAG 2 cut(s) 396, 710
Sfr274I CTCGAG 1 cut(s) 1368
SinI GGWCC 5 cut(s) 97, 106, 494, 608, 1285
SlaI CTCGAG 1 cut(s) 1368
SmiMI CAYNNNNRTG 2 cut(s) 1797, 2330
SmlI CTYRAG 2 cut(s) 1368, 2125
SmoI CTYRAG 2 cut(s) 1368, 2125
SpeI ACTAGT 1 cut(s) 2189
SphI GCATGC 1 cut(s) 636
SseBI AGGCCT 1 cut(s) 847
SsiI CCGC 4 cut(s) 209, 566, 1829, 1854
SspMI CTAG 8 cut(s) 14, 101, 773, 1445, 1463, 1587, 1811, 2190
StuI AGGCCT 1 cut(s) 847
StyD4I CCNGG 4 cut(s) 314, 1176, 1676, 2019
StyI CCWWGG 5 cut(s) 100, 220, 545, 1468, 1664
TaaI ACNGT 6 cut(s) 593, 1496, 1624, 1638, 1656, 2011
TaiI ACGT 6 cut(s) 268, 433, 605, 788, 888, 1693
TaqI TCGA 5 cut(s) 463, 687, 1233, 1250, 1369
TatI WGTACW 1 cut(s) 620
TauI GCSGC 1 cut(s) 212
TfiI GAWTC 4 cut(s) 227, 1252, 1754, 2360
Tru1I TTAA 7 cut(s) 50, 443, 1374, 1683, 1761, 1779, 2328
Tru9I TTAA 7 cut(s) 50, 443, 1374, 1683, 1761, 1779, 2328
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 3 cut(s) 596, 1577, 1880
Tsp45I GTSAC 1 cut(s) 325
Tth111I GACNNNGTC 1 cut(s) 698
Van91I CCANNNNNTGG 2 cut(s) 1304, 1694
VpaK11BI GGWCC 5 cut(s) 97, 106, 494, 608, 1285
XapI RAATTY 4 cut(s) 53, 962, 1228, 2152
XceI RCATGY 2 cut(s) 636, 1327
XcmI CCANNNNNNNNNTGG 1 cut(s) 91
XhoI CTCGAG 1 cut(s) 1368
XmaJI CCTAGG 1 cut(s) 100
XmiI GTMKAC 2 cut(s) 760, 930
XspI CTAG 8 cut(s) 14, 101, 773, 1445, 1463, 1587, 1811, 2190
ZrmI AGTACT 1 cut(s) 622
Zsp2I ATGCAT 2 cut(s) 155, 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.