Prupe.1G237200_v2.0.a1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000852
Physical Location & Seq
Reverse (-)
25174741 .. 25176419
1679 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G237200.1

Sequence Viewer

Length: 1374 bp
ATGCCTGCATTAGAAATTGAGGTGCTCTCAACAGAGACCGTCAAACCCTCCTCTCCAACCCCACCCCACCTCCGCCGCCACAACCTCTCCTTCATCGACCAACTCAACCCGCCAGTCTTCATGCCCATGGTCCTCTTCTTCCCTAAAGACCCCGACGCCACCGACTCCACATACATACAACAACGCTGCACAAGAATCAAACTAGCCTTATCCGAAACCCTAACCAAGTTCTACCCCTTAGCAGGACGCGTCAGGGAAAACCAATACATCGACTGCAACGATGAGGGTGCCCTCTACGCCGAAGCCAAAGCCAATGTCACCATCGCGGACGTCATCTCAAACCCTATCCCTAACGACTTCAACAAGTTCCTCCCATGCGAACTCGACTCCGCACACGAACTGGGCGTGTGCCTACAGGTCACGTCGTTCGTATGCGGCGGTATGAGCATCGCCATGGGTATGTCCCACAAGGCCGGAGACGCCTTGGCTTATTTCAGTTTTCTCAACAGCCTCGCCGCAGCCACGAGGGGCGAGGTCGACGCCGTACCCGCACCCGATTTTGTGTCCGACAAGTACTTCCCGCAAATGGACTTGTCCGGGTTTTACCACCCTACGAACGGGATGGTCAAAGACAACATTTCCACCAAACGTTTTGTGTTCGAGGCACCAGAAATTTCGACACTCAGGGAGAAATGCACCGGAGCCCAGAACGAACACGCTCGGCGTCCTACCCGTGTGGAGGCCTTATCGGCCTTCATATTGAGCCGATACGCAGCATCCTGCAACAACCCGAAGCAAATCGACAGATCTGATGATGTCCCGAAGACCTACATGGTGTCTCATGCGGTGAACTTGCGGACGAGAATGGATCCACCACTTCCGGAGTATACTTTCGGGAATTTGAGCCGAATGTCACAAGCGAGCCCACCAGTGCCCGTGGGCCCCAAAGACGGGTCGGTGTCCGACTCAACACTACAAGCCATAGTGAGCCACGTCAGAGAGGGGCTCAAGCAAGTCAACACGGAGTATGTAAAGAAATTGAAAGAAGGGCACCAGCACTTGAGTTTATTGAAAGAGCGCACCTCGCAAGTGAAACGAGGGGAGTTGGTGCCGTTTAGCTTCACGAGCCTTTGCAGGTTCCCGATGTACGACGCTGATTTTGGATGGGGGAAGGCTGTTTATATGGGGTCGGCCAGTTTGACGTTTAGAAACTTGGTGAGTTTCTTTGACACTCCAAGCGGCGATGGGGTGGAGGCGTGGGTTAATTTGAGGGAGGAAGACATGGAAATATTTGAAGCTGATGAGGAGTTTCTCAAATATGTTTCACCCAACCACAGTTGGAAGAAATCGGGGGTGAAAACTTTGATTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

458

Amino Acids

51.01

Weight (kDa)

5.93

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 333
Acc36I ACCTGC 1 cut(s) 1125
AccB1I GGYRCC 4 cut(s) 287, 664, 1050, 1108
AccI GTMKAC 2 cut(s) 537, 887
AccII CGCG 2 cut(s) 249, 326
AccIII TCCGGA 1 cut(s) 880
AclI AACGTT 1 cut(s) 649
AclWI GGATC 2 cut(s) 863, 876
AcoI YGGCCR 1 cut(s) 1191
AcsI RAATTY 2 cut(s) 672, 898
AcyI GRCGYC 5 cut(s) 156, 330, 480, 540, 724
AfaI GTAC 3 cut(s) 546, 575, 1148
AfiI CCNNNNNNNGG 6 cut(s) 242, 586, 617, 739, 950, 951
AflIII ACRYGT 1 cut(s) 247
AgsI TTSAA 4 cut(s) 361, 1042, 1072, 1295
AjiI CACGTC 2 cut(s) 423, 994
AluBI AGCT 2 cut(s) 1119, 1298
AluI AGCT 2 cut(s) 1119, 1298
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 3 cut(s) 29, 471, 843
AlwI GGATC 2 cut(s) 863, 876
Aor13HI TCCGGA 1 cut(s) 880
AoxI GGCC 5 cut(s) 471, 741, 750, 940, 1191
ApaI GGGCCC 1 cut(s) 944
ApeKI GCWGC 3 cut(s) 186, 518, 773
ApoI RAATTY 2 cut(s) 672, 898
ArsI GACNNNNNNTTYG 4 cut(s) 300, 332, 1142, 1174
AspLEI GCGC 1 cut(s) 1080
AspS9I GGNCC 3 cut(s) 130, 940, 941
AsuC2I CCSGG 1 cut(s) 598
AsuHPI GGTGA 5 cut(s) 310, 859, 1228, 1317, 1366
AvaII GGWCC 1 cut(s) 130
BaeGI GKGCMC 4 cut(s) 292, 936, 944, 1053
BamHI GGATCC 1 cut(s) 868
BanI GGYRCC 4 cut(s) 287, 664, 1050, 1108
BanII GRGCYC 4 cut(s) 706, 926, 944, 1008
BauI CACGAG 2 cut(s) 523, 1123
BbsI GAAGAC 3 cut(s) 109, 830, 1284
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 3 cut(s) 173, 530, 785
BccI CCATC 4 cut(s) 329, 616, 1158, 1238
BceAI ACGGC 2 cut(s) 527, 1096
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BcnI CCSGG 1 cut(s) 598
BcoDI GTCTC 3 cut(s) 29, 471, 843
BfaI CTAG 2 cut(s) 203, 1372
BfmI CTRYAG 1 cut(s) 413
BfuAI ACCTGC 1 cut(s) 1125
BglI GCCNNNNNGGC 1 cut(s) 749
BglII AGATCT 1 cut(s) 806
BisI GCNGC 7 cut(s) 76, 187, 436, 516, 519, 774, 1240
BlsI GCNGC 7 cut(s) 77, 188, 437, 517, 520, 775, 1241
BmcAI AGTACT 1 cut(s) 575
Bme1390I CCNGG 1 cut(s) 598
Bme18I GGWCC 1 cut(s) 130
BmgBI CACGTC 2 cut(s) 423, 994
BmgT120I GGNCC 3 cut(s) 130, 940, 941
BmiI GGNNCC 9 cut(s) 289, 666, 703, 870, 942, 943, 1052, 1110, 1139
BmrFI CCNGG 1 cut(s) 598
BmrI ACTGGG 1 cut(s) 410
BmsI GCATC 2 cut(s) 456, 785
BmuI ACTGGG 1 cut(s) 410
BpiI GAAGAC 3 cut(s) 109, 830, 1284
BplI GAGNNNNNCTC 6 cut(s) 11, 43, 990, 1022, 1067, 1099
Bpu10I CCTNAGC 1 cut(s) 238
BpuEI CTTGAG 2 cut(s) 992, 1081
BpuMI CCSGG 1 cut(s) 598
BsaHI GRCGYC 5 cut(s) 156, 330, 480, 540, 724
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 4 cut(s) 126, 453, 483, 936
BsaWI WCCGGW 2 cut(s) 698, 880
BsaXI ACNNNNNCTCC 4 cut(s) 54, 71, 84, 101
Bsc4I CCNNNNNNNGG 6 cut(s) 242, 586, 617, 739, 950, 951
Bse1I ACTGG 4 cut(s) 113, 405, 929, 1194
BseAI TCCGGA 1 cut(s) 880
BseDI CCNNGG 4 cut(s) 126, 453, 483, 936
BseGI GGATG 3 cut(s) 627, 776, 1169
BseLI CCNNNNNNNGG 6 cut(s) 242, 586, 617, 739, 950, 951
BseMII CTCAG 1 cut(s) 697
BseNI ACTGG 4 cut(s) 113, 405, 929, 1194
BseRI GAGGAG 2 cut(s) 40, 1319
BseSI GKGCMC 4 cut(s) 292, 936, 944, 1053
BseXI GCAGC 3 cut(s) 173, 530, 785
BsgI GTGCAG 1 cut(s) 172
Bsh1236I CGCG 2 cut(s) 249, 326
BshFI GGCC 5 cut(s) 473, 743, 752, 942, 1193
BshNI GGYRCC 4 cut(s) 287, 664, 1050, 1108
BsiHKAI GWGCWC 1 cut(s) 27
BsiSI CCGG 4 cut(s) 474, 597, 699, 881
BslFI GGGAC 2 cut(s) 448, 803
BslI CCNNNNNNNGG 6 cut(s) 242, 586, 617, 739, 950, 951
BsmAI GTCTC 3 cut(s) 29, 471, 843
BsmBI CGTCTC 1 cut(s) 471
BsmFI GGGAC 2 cut(s) 448, 803
BsnI GGCC 5 cut(s) 473, 743, 752, 942, 1193
Bso31I GGTCTC 1 cut(s) 29
Bsp120I GGGCCC 1 cut(s) 940
Bsp1286I GDGCHC 8 cut(s) 27, 292, 706, 926, 936, 944, 1008, 1053
Bsp13I TCCGGA 1 cut(s) 880
Bsp143I GATC 2 cut(s) 806, 868
Bsp19I CCATGG 2 cut(s) 126, 453
BspANI GGCC 5 cut(s) 473, 743, 752, 942, 1193
BspCNI CTCAG 1 cut(s) 696
BspEI TCCGGA 1 cut(s) 880
BspFNI CGCG 2 cut(s) 249, 326
BspLI GGNNCC 9 cut(s) 289, 666, 703, 870, 942, 943, 1052, 1110, 1139
BspMI ACCTGC 1 cut(s) 1125
BspPI GGATC 2 cut(s) 863, 876
BspT107I GGYRCC 4 cut(s) 287, 664, 1050, 1108
BspTNI GGTCTC 1 cut(s) 29
BsrI ACTGG 4 cut(s) 113, 405, 929, 1194
BssECI CCNNGG 4 cut(s) 126, 453, 483, 936
BssMI GATC 2 cut(s) 806, 868
BssNAI GTATAC 1 cut(s) 888
BssNI GRCGYC 5 cut(s) 156, 330, 480, 540, 724
BssSI CACGAG 2 cut(s) 523, 1123
BssT1I CCWWGG 3 cut(s) 126, 453, 483
Bst1107I GTATAC 1 cut(s) 888
Bst2BI CACGAG 2 cut(s) 523, 1123
Bst4CI ACNGT 2 cut(s) 40, 1337
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 5 cut(s) 156, 330, 480, 540, 724
BstC8I GCNNGC 2 cut(s) 6, 922
BstDEI CTNAG 2 cut(s) 238, 683
BstDSI CCRYGG 3 cut(s) 126, 453, 936
BstF5I GGATG 3 cut(s) 627, 776, 1169
BstFNI CGCG 2 cut(s) 249, 326
BstHHI GCGC 1 cut(s) 1080
BstKTI GATC 2 cut(s) 809, 871
BstMAI GTCTC 3 cut(s) 29, 471, 843
BstMBI GATC 2 cut(s) 806, 868
BstMWI GCNNNNNNNGC 7 cut(s) 296, 444, 479, 548, 749, 1084, 1125
BstSCI CCNGG 1 cut(s) 596
BstSFI CTRYAG 1 cut(s) 413
BstSLI GKGCMC 4 cut(s) 292, 936, 944, 1053
BstUI CGCG 2 cut(s) 249, 326
BstV1I GCAGC 3 cut(s) 173, 530, 785
BstV2I GAAGAC 3 cut(s) 109, 830, 1284
BstX2I RGATCY 2 cut(s) 806, 868
BstYI RGATCY 2 cut(s) 806, 868
BstZ17I GTATAC 1 cut(s) 888
BsuRI GGCC 5 cut(s) 473, 743, 752, 942, 1193
BtgI CCRYGG 3 cut(s) 126, 453, 936
BtgZI GCGATG 3 cut(s) 307, 433, 1257
BtrI CACGTC 2 cut(s) 423, 994
BtsCI GGATG 3 cut(s) 627, 776, 1169
BtsIMutI CAGTG 1 cut(s) 936
BveI ACCTGC 1 cut(s) 1125
Cac8I GCNNGC 2 cut(s) 6, 922
CfoI GCGC 1 cut(s) 1080
Cfr13I GGNCC 3 cut(s) 130, 940, 941
CseI GACGC 7 cut(s) 164, 238, 255, 488, 548, 713, 1160
Csp6I GTAC 3 cut(s) 545, 574, 1147
CviAII CATG 7 cut(s) 121, 127, 375, 454, 832, 842, 1282
CviQI GTAC 3 cut(s) 545, 574, 1147
DdeI CTNAG 2 cut(s) 238, 683
DpnI GATC 2 cut(s) 808, 870
DpnII GATC 2 cut(s) 806, 868
EaeI YGGCCR 1 cut(s) 1191
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
EciI GGCGGA 1 cut(s) 62
Eco130I CCWWGG 3 cut(s) 126, 453, 483
Eco147I AGGCCT 1 cut(s) 743
Eco24I GRGCYC 4 cut(s) 706, 926, 944, 1008
Eco31I GGTCTC 1 cut(s) 29
Eco47I GGWCC 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 941
EcoT14I CCWWGG 3 cut(s) 126, 453, 483
EcoT38I GRGCYC 4 cut(s) 706, 926, 944, 1008
ErhI CCWWGG 3 cut(s) 126, 453, 483
Esp3I CGTCTC 1 cut(s) 471
FaeI CATG 7 cut(s) 124, 130, 378, 457, 835, 845, 1285
FaqI GGGAC 2 cut(s) 448, 803
FatI CATG 7 cut(s) 120, 126, 374, 453, 831, 841, 1281
FauI CCCGC 3 cut(s) 117, 556, 588
FblI GTMKAC 2 cut(s) 537, 887
Fnu4HI GCNGC 7 cut(s) 76, 187, 436, 516, 519, 774, 1240
FokI GGATG 3 cut(s) 634, 763, 1176
FriOI GRGCYC 4 cut(s) 706, 926, 944, 1008
Fsp4HI GCNGC 7 cut(s) 76, 187, 436, 516, 519, 774, 1240
FspBI CTAG 2 cut(s) 203, 1372
GlaI GCGC 1 cut(s) 1079
GluI GCNGC 7 cut(s) 76, 187, 436, 516, 519, 774, 1240
HaeIII GGCC 5 cut(s) 473, 743, 752, 942, 1193
HapII CCGG 4 cut(s) 474, 597, 699, 881
HgaI GACGC 7 cut(s) 164, 238, 255, 488, 548, 713, 1160
HhaI GCGC 1 cut(s) 1080
Hin1I GRCGYC 5 cut(s) 156, 330, 480, 540, 724
Hin1II CATG 7 cut(s) 124, 130, 378, 457, 835, 845, 1285
Hin6I GCGC 1 cut(s) 1078
HinP1I GCGC 1 cut(s) 1078
HincII GTYRAC 2 cut(s) 538, 1018
HindII GTYRAC 2 cut(s) 538, 1018
HinfI GANTC 4 cut(s) 164, 195, 386, 965
HpaII CCGG 4 cut(s) 474, 597, 699, 881
HphI GGTGA 5 cut(s) 310, 859, 1228, 1317, 1366
Hpy166II GTNNAC 4 cut(s) 538, 850, 888, 1018
Hpy188I TCNGA 5 cut(s) 214, 568, 811, 964, 998
Hpy188III TCNNGA 5 cut(s) 820, 881, 895, 1123, 1141
Hpy8I GTNNAC 4 cut(s) 538, 850, 888, 1018
Hpy99I CGWCG 4 cut(s) 158, 427, 542, 1154
HpyAV CCTTC 4 cut(s) 100, 763, 1040, 1165
HpyCH4III ACNGT 2 cut(s) 40, 1337
HpyCH4IV ACGT 5 cut(s) 330, 422, 649, 993, 1202
HpyCH4V TGCA 6 cut(s) 8, 189, 276, 696, 783, 1134
HpyF10VI GCNNNNNNNGC 7 cut(s) 296, 444, 479, 548, 749, 1084, 1125
HpyF3I CTNAG 2 cut(s) 238, 683
HpySE526I ACGT 5 cut(s) 330, 422, 649, 993, 1202
Hsp92I GRCGYC 5 cut(s) 156, 330, 480, 540, 724
Hsp92II CATG 7 cut(s) 124, 130, 378, 457, 835, 845, 1285
HspAI GCGC 1 cut(s) 1078
Kpn2I TCCGGA 1 cut(s) 880
Kzo9I GATC 2 cut(s) 806, 868
LmnI GCTCC 1 cut(s) 701
Lsp1109I GCAGC 3 cut(s) 173, 530, 785
LweI GCATC 2 cut(s) 456, 785
MaeI CTAG 2 cut(s) 203, 1372
MaeII ACGT 5 cut(s) 330, 422, 649, 993, 1202
MaeIII GTNAC 3 cut(s) 316, 418, 912
MalI GATC 2 cut(s) 808, 870
MboI GATC 2 cut(s) 806, 868
MboII GAAGA 6 cut(s) 109, 127, 130, 835, 1289, 1354
MflI RGATCY 2 cut(s) 806, 868
MhlI GDGCHC 8 cut(s) 27, 292, 706, 926, 936, 944, 1008, 1053
MluCI AATT 5 cut(s) 15, 672, 898, 1037, 1264
MluI ACGCGT 1 cut(s) 247
MlyI GAGTC 3 cut(s) 158, 380, 959
MmeI TCCRAC 4 cut(s) 80, 591, 987, 1319
MroI TCCGGA 1 cut(s) 880
MseI TTAA 1 cut(s) 1263
MslI CAYNNNNRTG 3 cut(s) 125, 452, 458
MspI CCGG 4 cut(s) 474, 597, 699, 881
MspR9I CCNGG 1 cut(s) 598
MvnI CGCG 2 cut(s) 249, 326
MwoI GCNNNNNNNGC 7 cut(s) 296, 444, 479, 548, 749, 1084, 1125
NciI CCSGG 1 cut(s) 598
NcoI CCATGG 2 cut(s) 126, 453
NdeII GATC 2 cut(s) 806, 868
NlaIII CATG 7 cut(s) 124, 130, 378, 457, 835, 845, 1285
NlaIV GGNNCC 9 cut(s) 289, 666, 703, 870, 942, 943, 1052, 1110, 1139
NmeAIII GCCGAG 1 cut(s) 700
NmuCI GTSAC 3 cut(s) 316, 418, 912
PceI AGGCCT 1 cut(s) 743
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 402
PfeI GAWTC 1 cut(s) 195
PkrI GCNGC 7 cut(s) 77, 188, 437, 517, 520, 775, 1241
PleI GAGTC 3 cut(s) 158, 380, 959
PpsI GAGTC 3 cut(s) 158, 380, 959
Psp1406I AACGTT 1 cut(s) 649
PspN4I GGNNCC 9 cut(s) 289, 666, 703, 870, 942, 943, 1052, 1110, 1139
PspOMI GGGCCC 1 cut(s) 940
PspPI GGNCC 3 cut(s) 130, 940, 941
PsuI RGATCY 2 cut(s) 806, 868
RsaI GTAC 3 cut(s) 546, 575, 1148
RsaNI GTAC 3 cut(s) 545, 574, 1147
RseI CAYNNNNRTG 3 cut(s) 125, 452, 458
SalI GTCGAC 1 cut(s) 536
SaqAI TTAA 1 cut(s) 1263
SatI GCNGC 7 cut(s) 76, 187, 436, 516, 519, 774, 1240
Sau3AI GATC 2 cut(s) 806, 868
Sau96I GGNCC 3 cut(s) 130, 940, 941
ScaI AGTACT 1 cut(s) 575
SchI GAGTC 3 cut(s) 158, 380, 959
ScrFI CCNGG 1 cut(s) 598
SduI GDGCHC 8 cut(s) 27, 292, 706, 926, 936, 944, 1008, 1053
SfaNI GCATC 2 cut(s) 456, 785
SfcI CTRYAG 1 cut(s) 413
SfiI GGCCNNNNNGGCC 1 cut(s) 749
SinI GGWCC 1 cut(s) 130
SmiMI CAYNNNNRTG 3 cut(s) 125, 452, 458
SmlI CTYRAG 2 cut(s) 1007, 1060
SmoI CTYRAG 2 cut(s) 1007, 1060
Sse9I AATT 5 cut(s) 15, 672, 898, 1037, 1264
SseBI AGGCCT 1 cut(s) 743
SspI AATATT 1 cut(s) 1290
SspMI CTAG 2 cut(s) 203, 1372
StuI AGGCCT 1 cut(s) 743
StyD4I CCNGG 1 cut(s) 596
StyI CCWWGG 3 cut(s) 126, 453, 483
TaaI ACNGT 2 cut(s) 40, 1337
TaiI ACGT 5 cut(s) 333, 425, 652, 996, 1205
TaqI TCGA 7 cut(s) 96, 270, 384, 537, 660, 677, 801
TasI AATT 5 cut(s) 15, 672, 898, 1037, 1264
TatI WGTACW 1 cut(s) 573
TauI GCSGC 4 cut(s) 78, 438, 518, 1242
TfiI GAWTC 1 cut(s) 195
Tru1I TTAA 1 cut(s) 1263
Tru9I TTAA 1 cut(s) 1263
TscAI CASTG 1 cut(s) 936
TseFI GTSAC 3 cut(s) 316, 418, 912
TseI GCWGC 3 cut(s) 186, 518, 773
Tsp45I GTSAC 3 cut(s) 316, 418, 912
TspDTI ATGAA 3 cut(s) 82, 109, 745
TspGWI ACGGA 1 cut(s) 1037
TspRI CASTG 1 cut(s) 936
VpaK11BI GGWCC 1 cut(s) 130
XapI RAATTY 2 cut(s) 672, 898
XmiI GTMKAC 2 cut(s) 537, 887
XspI CTAG 2 cut(s) 203, 1372
ZraI GACGTC 1 cut(s) 331
ZrmI AGTACT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.