FvH4_5g27161

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000487
Physical Location & Seq
Forward (+)
18431177 .. 18438960
7784 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27161.t1

Sequence Viewer

Length: 2868 bp
ATGGATCAACGAACTCAAGTGTTTCTGCTTCTTATATACGCTCCGGTTCTCGTTTTATCCAATCATCAGACAAGTATTCCGGATGTTACTGGTTTAAAGTCTCTAATGGCACTGTGGACTAACACACCCCCAAGTTGGGGAGCTCGCGATCCTTGTGAAGATAGTTGGGATGGAATTGGGTGTACCAACATGCGTGTCGTCTCTTTAACATTATCGAGTATGAATTTGAGTGGCTCACTCTCTGGAGATATTCAACAGTTATCTGAACTACAAATTTTGGATCTCTCTTACAACAAGGGCCTGTCAGGACCACTCCCAACACAAATTGGGAGTTTGACGAAGCTTACTAACATAATCCTGGTTGGTTGCAGTTTCTCTGGTCTCATCCCTGCTTCAATAGGGTCCCTACAAGAGCTAAGATTTCTATCTCTGAATGCTAACAGATTTAGTGGGAAGATCCCATCTTCCATTGGTAATCTGTCTAAACTATATTGGCTGGATCTAGCTGACAACAACCTCGAAGGACCCATCCCAGTTTCTGATGGTGTTGAACCTGGACTTGACATGCTCAGTCATTGCAAGCACTTTCATCTCGGAATCAATCAGCTCTCAGGCCCAATTCCATCAAAGCTCTTTAACTCAAACATGAGTCTGATACATGTGCTCTTCGAAAGTAATAAACTCACTGGCAGCATCCCTTCCAGCATTGGACTTCTAAAGACTTTGGAGGTGGTGCGATTTGACAGAAACATGTTAAGTGGGCCTATTCCTCCGAGCCTCAGCAATCTTACAAGTGCTTCCCAGCTGTTCTTGTCCAACAACAAGCTGAGCGGCCCTCCTCCTGACCTTACTGGCATGATCCTTCTCCAGTATCTGGATTTAAGCAATAATAGTTTCGACTTGTCTGATATTCCACCGTGGTTTTCAACACTAGAGTTTTTGACCACATTAATCATGGCGGACACACAACTTCAAGGAGAAATACCTGCTGCTCTATTCAGGCTTTCAAACATACAGACTGTGGTCCTGAGGAACAACCAGCTGAATGGCACTTTGGATATTGTCAATACCTCCAGCAGTCAATTGACTCTCGTTGATTTGCAAAATAATTTAATAAGCGAATACAAAGGAAATGGAGGATACCGTGGTACTTTGATACTTGTTCAAAATCCAGTTTGTGACGAAACTGGAGCTTCACTGAGTTACTGCAAGATTTCGCTATCATCTGATATCGTGCCATATGAAACAATGCCAAATAACTGTGTGCCCAGTCTCTGCAGCTCCAATCAGGTTTCAAGCCCCAACTGTAAATGTGCATATCCATACAAGGGCACTCTAATCTTTAGAGCTCCTACCTTCTCAGACTTGGAAAACTCGACTCACTACAAAGAGCTTGAGAAGGCACTCACGAATTCTTTCTTATCCCATCAACTTAGTGTCGATTCTGTATCTTTGAGTAATCTCACCAAGGATTCACTTTCATACCTTAAGTTGGCTCTAGCAGTATTTCCACAAAGCCAAGATCGTTTCAATAGAAAGGAAGTTTCTAGTATTTCTTTTCTACTCAGTAACCAAACATTCAAGCCTCAAAAATCTTTTGGACCCTTCTATTTCATTGGTGACGATTACGAATACTTTGAAGTTGAAGTACCATCAGAATCAAACAAATCGTCTAATGTGGGCATTATTATTGGAGCAGCAGCTGGTGGTTCTGTTCTTGTGCTAATATTAATTCTTGTAGGAATTTATGCAATTCACCAGAAAAGAAGGGCAAAAAGAGCCACTGATAAAAACAATCCTTTTGGCAAATGGGATGTGAATGATAAGAGCAGGGGTAGCGTTCCTCAATTAAATGGTGCTAGATCGTTCTCTTTTGAAGAACTCATGCAATACAGCAACAACTTTTCAGAAGAAAATGATATTGGATCTGGGGGCTATGGAAAGGTTTACCGTGGAATTCTTCCCACAGGGCAACTGATTGCCATCAAACGAGCTCACACAGAATCTATTCAAGGTGGAAATCAGTTCAGAACTGAGATTGAACTTCTTTCACGGGTTCATCATAAGAACCTTGTCAGCCTTCTAGGATTTTGTTTTGACCGGGGTGAGCAGATGCTGGTGTATGAGTATGTAGCAAATGGGACTCTTAAGGATAGTCTTTCAGGTAAGTCAGGAATCAGATTAGATTGGCAGAGGAGACTTAAAATAGCACTTGGTACAGCTAGAGGCTTAGCCTATCTTCATGAACTTTGCGACCCTCCAATTATACACAGAGATATAAAATCAACTAATGTTCTGTTGGACAACCACTTGAATGCAAAAGTTGCTGATTTCGGTCTCTCCAAGTCCATGGGTAACAGTGGGAGGGATCATGTCTCCACTCAAGTTAAAGGGACAATGGGATACATGGATCCTGAATATTACATGACGCAACGGTTGACTGAGAAGAGTGATGTTTATGGTTTTGGAGTGCTAATGTTGGAGCTCCTTACTGCAAGAAGACCCATAGAACAAGGAAAATATATCGTGCGAGAAGTAAGGACGGCAATGGACAAGACTAAAGAATTATACAACCTTCATGACATCCTGGATTTAGCTATTGGTTTGGGGACGACACTCAAAGGTTTAGAGAGGTTTGTTGATCTTGCAATGACTTGTGTTGAAGAAGAAGGAGCCAACAGGCCTACAATGAGTGAGGTGGTAAAAGAGATTGAAAACATTATGCAGCTTGCTGGTCTGAACCCTAATGCAGAGTCAGCATCCACTTCCGCAAGCTACGAGGATCAGGCAAGCACTAAGCATCCTTACAGTGATGAGGCCTTTGATTATAGTGGAGGATTTCCTGCTTCAAAGATAGAGCCGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 Pkinase Pkinase_Tyr

Protein Analysis

956

Amino Acids

104.94

Weight (kDa)

5.74

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 636 - 902 1.6e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 638 - 903 5.5e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49760 AT5G49770
fragaria_vesca FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23881 FvH4_3g23881 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_5g27161 FvH4_5g27170
malus_domestica MD11G1206300.v1.1
prunus_persica Prupe.4G230700_v2.0.a1 Prupe.4G230700_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1
pyrus_communis pycom11g17930
rosa_chinensis RchiOBHm_Chr5g0042211 RchiOBHm_Chr5g0042261 RchiOBHm_Chr5g0042271 RchiOBHm_Chr5g0042311 RchiOBHm_Chr5g0042381 RchiOBHm_Chr7g0218261
rosa_laevigata RLG00000002384 RLG00000002385 RLG00000034112 RLG00000034114 RLG00000034116 RLG00000034118
rosa_multiflora Rmu_sc0001715.1_g000008 Rmu_sc0001715.1_g000012 Rmu_sc0001715.1_g000026 Rmu_sc0004956.1_g000008
rosa_roxburghii Rroxscaffold_1G00038360 Rroxscaffold_1G00038390 Rroxscaffold_1G00038400 Rroxscaffold_1G00038450 Rroxscaffold_3G00241180
rosa_rugosa Rorug05G0198800 Rorug05G0199000 Rorug05G0199100.1 Rorug05G0199200 Rorug05G0199300 Rorug07G0175900
rosa_samantha Rh5AG283400 Rh5AG283900 Rh5AG284100 Rh5AG284300 Rh5AG284900 Rh5AG285300 Rh5AG285700 Rh5BG289300 Rh5BG289900 Rh5BG290200 Rh5BG290400 Rh5CG320700 Rh5CG321200 Rh5CG321500 Rh5CG322100 Rh5CG322400 Rh5DG298600 Rh5DG299100 Rh5DG299400 Rh5DG300200 Rh7AG317500 Rh7BG308900 Rh7CG334400 Rh7DG316500
rosa_wichuraiana Rw0G001480 Rw0G001500 Rw0G001750 Rw0G001780 Rw0G015430 Rw5G026520 Rw7G026960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 994
AccB7I CCANNNNNTGG 1 cut(s) 874
AccBSI CCGCTC 1 cut(s) 831
AccII CGCG 1 cut(s) 147
AccIII TCCGGA 1 cut(s) 79
AciI CCGC 4 cut(s) 831, 959, 2769, 2861
AcsI RAATTY 5 cut(s) 223, 273, 1411, 1743, 1956
AfaI GTAC 4 cut(s) 184, 1150, 1650, 2218
AfiI CCNNNNNNNGG 6 cut(s) 135, 136, 137, 874, 1328, 1492
AflII CTTAAG 2 cut(s) 1487, 2147
AflIII ACRYGT 2 cut(s) 658, 750
AjnI CCWGG 3 cut(s) 357, 553, 2586
AjuI GAANNNNNNNTTGG 4 cut(s) 1037, 1069, 1905, 1937
Alw21I GWGCWC 5 cut(s) 145, 666, 1351, 1996, 2487
Alw26I GTCTC 7 cut(s) 105, 205, 386, 1277, 2191, 2342, 2380
AlwNI CAGNNNCTG 5 cut(s) 539, 874, 1275, 1703, 2116
Aor13HI TCCGGA 1 cut(s) 79
AoxI GGCC 6 cut(s) 298, 613, 761, 832, 2681, 2817
ApeKI GCWGC 6 cut(s) 690, 989, 1278, 1697, 1700, 2725
ApoI RAATTY 5 cut(s) 223, 273, 1411, 1743, 1956
ArsI GACNNNNNNTTYG 2 cut(s) 1655, 1687
AseI ATTAAT 2 cut(s) 950, 1730
Asp700I GAANNNNTTC 2 cut(s) 1415, 2007
AspS9I GGNCC 9 cut(s) 298, 308, 402, 524, 614, 761, 833, 1024, 1601
AsuC2I CCSGG 1 cut(s) 2102
AsuHPI GGTGA 4 cut(s) 1456, 1631, 1748, 2117
AsuII TTCGAA 1 cut(s) 669
AvaII GGWCC 5 cut(s) 308, 402, 524, 1024, 1601
AxyI CCTNAGG 1 cut(s) 1028
BaeGI GKGCMC 2 cut(s) 1269, 1334
BaeI ACNNNNGTAYC 2 cut(s) 1132, 1165
BamHI GGATCC 1 cut(s) 2410
BanII GRGCYC 4 cut(s) 145, 1351, 1996, 2487
BarI GAAGNNNNNNTAC 2 cut(s) 1632, 1664
BbsI GAAGAC 1 cut(s) 2506
Bbv12I GWGCWC 5 cut(s) 145, 666, 1351, 1996, 2487
BbvCI CCTCAGC 1 cut(s) 779
BbvI GCAGC 6 cut(s) 702, 976, 1290, 1709, 1712, 2737
BccI CCATC 8 cut(s) 164, 469, 536, 536, 631, 1434, 1660, 1991
BceAI ACGGC 1 cut(s) 2559
BciT130I CCWGG 3 cut(s) 359, 555, 2588
BciVI GTATCC 2 cut(s) 1133, 2396
BcnI CCSGG 1 cut(s) 2102
BcoDI GTCTC 7 cut(s) 105, 205, 386, 1277, 2191, 2342, 2380
BfaI CTAG 7 cut(s) 503, 932, 1499, 1548, 1860, 2084, 2223
BfmI CTRYAG 2 cut(s) 1276, 2864
BfrI CTTAAG 2 cut(s) 1487, 2147
BfuAI ACCTGC 1 cut(s) 994
BfuI GTATCC 2 cut(s) 1133, 2396
BisI GCNGC 8 cut(s) 691, 832, 990, 1279, 1698, 1701, 2726, 2861
BlpI GCTNAGC 2 cut(s) 827, 2230
BlsI GCNGC 8 cut(s) 692, 833, 991, 1280, 1699, 1702, 2727, 2862
Bme1390I CCNGG 4 cut(s) 359, 555, 2102, 2588
Bme18I GGWCC 5 cut(s) 308, 402, 524, 1024, 1601
BmgT120I GGNCC 9 cut(s) 298, 308, 402, 524, 614, 761, 833, 1024, 1601
BmiI GGNNCC 6 cut(s) 403, 404, 526, 1603, 2412, 2674
BmrFI CCNGG 4 cut(s) 359, 555, 2102, 2588
BmrI ACTGGG 2 cut(s) 527, 1263
BmsI GCATC 4 cut(s) 702, 2103, 2768, 2809
BmuI ACTGGG 2 cut(s) 527, 1263
BoxI GACNNNNGTC 1 cut(s) 1022
BpiI GAAGAC 1 cut(s) 2506
BplI GAGNNNNNCTC 2 cut(s) 820, 852
BpmI CTGGAG 4 cut(s) 264, 851, 1057, 1209
Bpu10I CCTNAGC 1 cut(s) 779
Bpu1102I GCTNAGC 2 cut(s) 827, 2230
Bpu14I TTCGAA 1 cut(s) 669
BpuEI CTTGAG 2 cut(s) 1415, 2367
BpuMI CCSGG 1 cut(s) 2102
BsaBI GATNNNNATC 2 cut(s) 424, 1982
BsaI GGTCTC 2 cut(s) 386, 2342
BsaJI CCNNGG 6 cut(s) 917, 1144, 1467, 1951, 2101, 2349
BsaWI WCCGGW 2 cut(s) 43, 79
Bsc4I CCNNNNNNNGG 6 cut(s) 135, 136, 137, 874, 1328, 1492
Bse1I ACTGG 8 cut(s) 94, 533, 691, 856, 868, 1172, 1192, 1269
Bse21I CCTNAGG 1 cut(s) 1028
Bse3DI GCAATG 3 cut(s) 574, 2553, 2655
Bse8I GATNNNNATC 2 cut(s) 424, 1982
BseAI TCCGGA 1 cut(s) 79
BseBI CCWGG 3 cut(s) 359, 555, 2588
BseDI CCNNGG 6 cut(s) 917, 1144, 1467, 1951, 2101, 2349
BseGI GGATG 9 cut(s) 88, 175, 384, 528, 693, 1819, 2583, 2759, 2800
BseJI GATNNNNATC 2 cut(s) 424, 1982
BseLI CCNNNNNNNGG 6 cut(s) 135, 136, 137, 874, 1328, 1492
BseMI GCAATG 3 cut(s) 574, 2553, 2655
BseNI ACTGG 8 cut(s) 94, 533, 691, 856, 868, 1172, 1192, 1269
BseRI GAGGAG 2 cut(s) 828, 2209
BseSI GKGCMC 2 cut(s) 1269, 1334
BseXI GCAGC 6 cut(s) 702, 976, 1290, 1709, 1712, 2737
BseYI CCCAGC 1 cut(s) 801
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 6 cut(s) 300, 615, 763, 834, 2683, 2819
BsiHKAI GWGCWC 5 cut(s) 145, 666, 1351, 1996, 2487
BsiSI CCGG 3 cut(s) 44, 80, 2101
BslFI GGGAC 4 cut(s) 388, 2155, 2407, 2623
BslI CCNNNNNNNGG 6 cut(s) 135, 136, 137, 874, 1328, 1492
BsmAI GTCTC 7 cut(s) 105, 205, 386, 1277, 2191, 2342, 2380
BsmBI CGTCTC 1 cut(s) 205
BsmFI GGGAC 4 cut(s) 388, 2155, 2407, 2623
BsmI GAATGC 2 cut(s) 439, 2320
BsnI GGCC 6 cut(s) 300, 615, 763, 834, 2683, 2819
Bso31I GGTCTC 2 cut(s) 386, 2342
Bsp119I TTCGAA 1 cut(s) 669
Bsp1286I GDGCHC 7 cut(s) 145, 666, 1269, 1334, 1351, 1996, 2487
Bsp13I TCCGGA 1 cut(s) 79
Bsp1720I GCTNAGC 2 cut(s) 827, 2230
Bsp19I CCATGG 1 cut(s) 2349
Bsp68I TCGCGA 1 cut(s) 147
BspACI CCGC 4 cut(s) 831, 959, 2769, 2861
BspANI GGCC 6 cut(s) 300, 615, 763, 834, 2683, 2819
BspEI TCCGGA 1 cut(s) 79
BspFNI CGCG 1 cut(s) 147
BspHI TCATGA 2 cut(s) 2242, 2578
BspLI GGNNCC 6 cut(s) 403, 404, 526, 1603, 2412, 2674
BspMAI CTGCAG 1 cut(s) 1280
BspMI ACCTGC 1 cut(s) 994
BspQI GCTCTTC 1 cut(s) 671
BspT104I TTCGAA 1 cut(s) 669
BspTI CTTAAG 2 cut(s) 1487, 2147
BspTNI GGTCTC 2 cut(s) 386, 2342
BsrBI CCGCTC 1 cut(s) 831
BsrDI GCAATG 3 cut(s) 574, 2553, 2655
BsrI ACTGG 8 cut(s) 94, 533, 691, 856, 868, 1172, 1192, 1269
BssECI CCNNGG 6 cut(s) 917, 1144, 1467, 1951, 2101, 2349
BssT1I CCWWGG 2 cut(s) 1467, 2349
Bst2UI CCWGG 3 cut(s) 359, 555, 2588
Bst6I CTCTTC 2 cut(s) 671, 2441
BstAFI CTTAAG 2 cut(s) 1487, 2147
BstAPI GCANNNNNTGC 1 cut(s) 2324
BstBI TTCGAA 1 cut(s) 669
BstC8I GCNNGC 5 cut(s) 145, 581, 2730, 2773, 2791
BstDSI CCRYGG 4 cut(s) 917, 1144, 1951, 2349
BstF5I GGATG 9 cut(s) 88, 175, 384, 528, 693, 1819, 2583, 2759, 2800
BstFNI CGCG 1 cut(s) 147
BstMAI GTCTC 7 cut(s) 105, 205, 386, 1277, 2191, 2342, 2380
BstMWI GCNNNNNNNGC 4 cut(s) 1778, 1836, 2324, 2756
BstNI CCWGG 3 cut(s) 359, 555, 2588
BstNSI RCATGY 4 cut(s) 193, 568, 662, 754
BstPAI GACNNNNGTC 1 cut(s) 1022
BstSCI CCNGG 4 cut(s) 357, 553, 2100, 2586
BstSFI CTRYAG 2 cut(s) 1276, 2864
BstSLI GKGCMC 2 cut(s) 1269, 1334
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 6 cut(s) 702, 976, 1290, 1709, 1712, 2737
BstV2I GAAGAC 1 cut(s) 2506
BstX2I RGATCY 5 cut(s) 280, 456, 499, 1925, 2410
BstXI CCANNNNNNTGG 2 cut(s) 1046, 2350
BstYI RGATCY 5 cut(s) 280, 456, 499, 1925, 2410
Bsu36I CCTNAGG 1 cut(s) 1028
BsuI GTATCC 2 cut(s) 1133, 2396
BsuRI GGCC 6 cut(s) 300, 615, 763, 834, 2683, 2819
BtgI CCRYGG 4 cut(s) 917, 1144, 1951, 2349
BtsCI GGATG 9 cut(s) 88, 175, 384, 528, 693, 1819, 2583, 2759, 2800
BtsIMutI CAGTG 6 cut(s) 110, 684, 1196, 1782, 2365, 2815
BtuMI TCGCGA 1 cut(s) 147
BveI ACCTGC 1 cut(s) 994
Cac8I GCNNGC 5 cut(s) 145, 581, 2730, 2773, 2791
CaiI CAGNNNCTG 5 cut(s) 539, 874, 1275, 1703, 2116
CciI TCATGA 2 cut(s) 2242, 2578
Cfr13I GGNCC 9 cut(s) 298, 308, 402, 524, 614, 761, 833, 1024, 1601
CseI GACGC 1 cut(s) 2437
Csp6I GTAC 4 cut(s) 183, 1149, 1649, 2217
CviQI GTAC 4 cut(s) 183, 1149, 1649, 2217
DraI TTTAAA 1 cut(s) 96
Eam1104I CTCTTC 2 cut(s) 671, 2441
EarI CTCTTC 2 cut(s) 671, 2441
EciI GGCGGA 1 cut(s) 974
Ecl136II GAGCTC 4 cut(s) 143, 1349, 1994, 2485
Eco130I CCWWGG 2 cut(s) 1467, 2349
Eco147I AGGCCT 2 cut(s) 2683, 2819
Eco24I GRGCYC 4 cut(s) 145, 1351, 1996, 2487
Eco31I GGTCTC 2 cut(s) 386, 2342
Eco32I GATATC 1 cut(s) 1231
Eco47I GGWCC 5 cut(s) 308, 402, 524, 1024, 1601
Eco53kI GAGCTC 4 cut(s) 143, 1349, 1994, 2485
Eco81I CCTNAGG 1 cut(s) 1028
EcoICRI GAGCTC 4 cut(s) 143, 1349, 1994, 2485
EcoO109I RGGNCCY 3 cut(s) 298, 402, 524
EcoRI GAATTC 2 cut(s) 1411, 1956
EcoRII CCWGG 3 cut(s) 357, 553, 2586
EcoRV GATATC 1 cut(s) 1231
EcoT14I CCWWGG 2 cut(s) 1467, 2349
EcoT38I GRGCYC 4 cut(s) 145, 1351, 1996, 2487
ErhI CCWWGG 2 cut(s) 1467, 2349
Esp3I CGTCTC 1 cut(s) 205
FaqI GGGAC 4 cut(s) 388, 2155, 2407, 2623
FauNDI CATATG 1 cut(s) 1240
Fnu4HI GCNGC 8 cut(s) 691, 832, 990, 1279, 1698, 1701, 2726, 2861
FokI GGATG 9 cut(s) 95, 182, 371, 515, 680, 1826, 2570, 2746, 2787
FriOI GRGCYC 4 cut(s) 145, 1351, 1996, 2487
Fsp4HI GCNGC 8 cut(s) 691, 832, 990, 1279, 1698, 1701, 2726, 2861
FspBI CTAG 7 cut(s) 503, 932, 1499, 1548, 1860, 2084, 2223
GluI GCNGC 8 cut(s) 691, 832, 990, 1279, 1698, 1701, 2726, 2861
GsaI CCCAGC 1 cut(s) 805
GsuI CTGGAG 4 cut(s) 264, 851, 1057, 1209
HaeIII GGCC 6 cut(s) 300, 615, 763, 834, 2683, 2819
HapII CCGG 3 cut(s) 44, 80, 2101
HgaI GACGC 1 cut(s) 2437
HincII GTYRAC 1 cut(s) 2439
HindII GTYRAC 1 cut(s) 2439
HindIII AAGCTT 1 cut(s) 341
HpaII CCGG 3 cut(s) 44, 80, 2101
HphI GGTGA 4 cut(s) 1456, 1631, 1748, 2117
Hpy166II GTNNAC 4 cut(s) 117, 183, 1948, 2439
Hpy8I GTNNAC 4 cut(s) 117, 183, 1948, 2439
HpyF10VI GCNNNNNNNGC 4 cut(s) 1778, 1836, 2324, 2756
KflI GGGWCCC 1 cut(s) 402
Kpn2I TCCGGA 1 cut(s) 79
LguI GCTCTTC 1 cut(s) 671
LmnI GCTCC 9 cut(s) 46, 140, 1190, 1286, 1354, 1694, 2482, 2490, 2672
Lsp1109I GCAGC 6 cut(s) 702, 976, 1290, 1709, 1712, 2737
LweI GCATC 4 cut(s) 702, 2103, 2768, 2809
MaeI CTAG 7 cut(s) 503, 932, 1499, 1548, 1860, 2084, 2223
MaeIII GTNAC 6 cut(s) 85, 1178, 1202, 1568, 1619, 2354
MbiI CCGCTC 1 cut(s) 831
MfeI CAATTG 1 cut(s) 1082
MflI RGATCY 5 cut(s) 280, 456, 499, 1925, 2410
MhlI GDGCHC 7 cut(s) 145, 666, 1269, 1334, 1351, 1996, 2487
MlyI GAGTC 5 cut(s) 658, 1081, 1372, 2137, 2762
MmeI TCCRAC 3 cut(s) 840, 2280, 2460
MroI TCCGGA 1 cut(s) 79
MroXI GAANNNNTTC 2 cut(s) 1415, 2007
MslI CAYNNNNRTG 1 cut(s) 2313
MspA1I CMGCKG 4 cut(s) 805, 1042, 1703, 2863
MspCI CTTAAG 2 cut(s) 1487, 2147
MspI CCGG 3 cut(s) 44, 80, 2101
MspR9I CCNGG 4 cut(s) 359, 555, 2102, 2588
MunI CAATTG 1 cut(s) 1082
Mva1269I GAATGC 2 cut(s) 439, 2320
MvaI CCWGG 3 cut(s) 359, 555, 2588
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 4 cut(s) 1778, 1836, 2324, 2756
NciI CCSGG 1 cut(s) 2102
NcoI CCATGG 1 cut(s) 2349
NdeI CATATG 1 cut(s) 1240
NlaIV GGNNCC 6 cut(s) 403, 404, 526, 1603, 2412, 2674
NmuCI GTSAC 2 cut(s) 1178, 1619
NruI TCGCGA 1 cut(s) 147
NspI RCATGY 4 cut(s) 193, 568, 662, 754
NspV TTCGAA 1 cut(s) 669
PagI TCATGA 2 cut(s) 2242, 2578
PceI AGGCCT 2 cut(s) 2683, 2819
PciI ACATGT 2 cut(s) 658, 750
PciSI GCTCTTC 1 cut(s) 671
PctI GAATGC 2 cut(s) 439, 2320
PdmI GAANNNNTTC 2 cut(s) 1415, 2007
PfeI GAWTC 6 cut(s) 597, 1442, 1472, 1658, 2003, 2175
PflMI CCANNNNNTGG 1 cut(s) 874
PfoI TCCNGGA 1 cut(s) 2586
PkrI GCNGC 8 cut(s) 692, 833, 991, 1280, 1699, 1702, 2727, 2862
PleI GAGTC 5 cut(s) 657, 1081, 1372, 2137, 2761
PpsI GAGTC 5 cut(s) 657, 1081, 1372, 2137, 2761
PpuMI RGGWCCY 2 cut(s) 402, 524
PscI ACATGT 2 cut(s) 658, 750
PshAI GACNNNNGTC 1 cut(s) 1022
PshBI ATTAAT 2 cut(s) 950, 1730
Psp124BI GAGCTC 4 cut(s) 145, 1351, 1996, 2487
Psp5II RGGWCCY 2 cut(s) 402, 524
Psp6I CCWGG 3 cut(s) 357, 553, 2586
PspFI CCCAGC 1 cut(s) 801
PspGI CCWGG 3 cut(s) 357, 553, 2586
PspN4I GGNNCC 6 cut(s) 403, 404, 526, 1603, 2412, 2674
PspPI GGNCC 9 cut(s) 298, 308, 402, 524, 614, 761, 833, 1024, 1601
PspPPI RGGWCCY 2 cut(s) 402, 524
PstI CTGCAG 1 cut(s) 1280
PstNI CAGNNNCTG 5 cut(s) 539, 874, 1275, 1703, 2116
PsuI RGATCY 5 cut(s) 280, 456, 499, 1925, 2410
PvuII CAGCTG 3 cut(s) 805, 1042, 1703
RruI TCGCGA 1 cut(s) 147
RsaI GTAC 4 cut(s) 184, 1150, 1650, 2218
RsaNI GTAC 4 cut(s) 183, 1149, 1649, 2217
RseI CAYNNNNRTG 1 cut(s) 2313
SacI GAGCTC 4 cut(s) 145, 1351, 1996, 2487
SapI GCTCTTC 1 cut(s) 671
SatI GCNGC 8 cut(s) 691, 832, 990, 1279, 1698, 1701, 2726, 2861
Sau96I GGNCC 9 cut(s) 298, 308, 402, 524, 614, 761, 833, 1024, 1601
SchI GAGTC 5 cut(s) 658, 1081, 1372, 2137, 2762
ScrFI CCNGG 4 cut(s) 359, 555, 2102, 2588
SduI GDGCHC 7 cut(s) 145, 666, 1269, 1334, 1351, 1996, 2487
SfaNI GCATC 4 cut(s) 702, 2103, 2768, 2809
SfcI CTRYAG 2 cut(s) 1276, 2864
SfuI TTCGAA 1 cut(s) 669
SinI GGWCC 5 cut(s) 308, 402, 524, 1024, 1601
SmiMI CAYNNNNRTG 1 cut(s) 2313
SmlI CTYRAG 5 cut(s) 15, 1394, 1487, 2147, 2382
SmoI CTYRAG 5 cut(s) 15, 1394, 1487, 2147, 2382
SseBI AGGCCT 2 cut(s) 2683, 2819
SsiI CCGC 4 cut(s) 831, 959, 2769, 2861
SspI AATATT 2 cut(s) 1728, 2420
SspMI CTAG 7 cut(s) 503, 932, 1499, 1548, 1860, 2084, 2223
SstI GAGCTC 4 cut(s) 145, 1351, 1996, 2487
StuI AGGCCT 2 cut(s) 2683, 2819
StyD4I CCNGG 4 cut(s) 357, 553, 2100, 2586
StyI CCWWGG 2 cut(s) 1467, 2349
TaqI TCGA 6 cut(s) 215, 519, 669, 897, 1376, 1440
TaqII GACCGA 1 cut(s) 2324
TauI GCSGC 2 cut(s) 834, 2863
TfiI GAWTC 6 cut(s) 597, 1442, 1472, 1658, 2003, 2175
TscAI CASTG 6 cut(s) 117, 691, 1203, 1789, 2365, 2815
TseFI GTSAC 2 cut(s) 1178, 1619
TseI GCWGC 6 cut(s) 690, 989, 1278, 1697, 1700, 2725
Tsp45I GTSAC 2 cut(s) 1178, 1619
TspDTI ATGAA 9 cut(s) 236, 578, 1257, 1470, 1603, 2048, 2231, 2259, 2567
TspRI CASTG 6 cut(s) 117, 691, 1203, 1789, 2365, 2815
Van91I CCANNNNNTGG 1 cut(s) 874
Vha464I CTTAAG 2 cut(s) 1487, 2147
VpaK11BI GGWCC 5 cut(s) 308, 402, 524, 1024, 1601
VspI ATTAAT 2 cut(s) 950, 1730
XapI RAATTY 5 cut(s) 223, 273, 1411, 1743, 1956
XceI RCATGY 4 cut(s) 193, 568, 662, 754
XcmI CCANNNNNNNNNTGG 1 cut(s) 952
XmnI GAANNNNTTC 2 cut(s) 1415, 2007
XspI CTAG 7 cut(s) 503, 932, 1499, 1548, 1860, 2084, 2223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.