RchiOBHm_Chr7g0218261

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000487
Physical Location & Seq
Forward (+)
36265807 .. 36272515
6709 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19537

Sequence Viewer

Length: 2865 bp
ATGGATCATAGAATTCAGGTGTTTCTGCTTCTTATATTCACGCCTTTTCTCGTTTTAGCAAACCATGAGACAAGTTCTCTGGATGTTACTGGTTTAAAATCTCTAAGGGCACTATGGACCAACACGCCTCCAAGTTGGTGGACTCTGGATCCTTGTGAAGATGGTTGGGATGGCGTTGGGTGTCACAACATGCGTGTGGTCTCTTTAACATTACCGAGTATGAATTTGACTGGCACACTCTCTGGAGATATTGGACAGTTATCTGAACTACAGGTTTTGGATCTATCTTACAACAAGGGCCTGACAGGACCACTCCCAGGAGAAATTAGCAGTTTGAAGAAGCTATCTAACATAATTCTGGTTGGTTGCGGCTTCTCTGGTCCCATACCTCCTACTATAGGGTCCCTACAGGAGCTAATATTTCTGTCTCTGAATTCTAACGGATTTAGTGGGAGCATCCCATCTTCCATTGGTAATCTGTCTAAACTATATTGGCTGGATCTAGCTGACAACCACCTTGATGGACCCATCCCAGTTTCTCATGGTGGTGAACCTGGTCTTGACATGCTCAGTCATTGCAAGCACTTTCATCTCGGAATGAATAAGCTCTCAGGCCCAATTCCATCAAAGCTTTTTAGCTCAAACATGAGCCTGATACATGTGCTCTTCGAAAGTAATAAACTCACTGGCAGCATCCCTTCCAGCCTTGGACTTGTAACGTCTTTGGAGGTTGTGCGATTTGATGGAAACATGTTAACTGGGCCTATTCCTCTGAGCCTCAACAGTCTTACAAATGTTTCTCAGCTGTTCTTGTCTAATAACAAGCTGAGCGGCCCTGCTCCCAACCTTACTGGCATGAATCTTCTCCATTATGTAGATTTGAGCAATAATAGTTTCGACGTGTCTGATGTTCCACCATGGTTTTCAACACTAGAGTATTTGACCACATTAATGATGGAGGACACACAACTTCAAGGAGAAATACCTGCTGCTCTATTCAGCCTTTTAAACCTACAGACTGTGGTCCTGAGGAACAACCAGCTCAATGGCACCTTGGATATTGGCACTACGAGCAGTCAATTGCAACTCATTGATTTACAAAAGAATTTAATTAAGGAATACGAAGAAAATGGAGGATACCATGGTACTTTAATACTTGCTCAAAATCCAGTTTGTGACGAAACTGGAGCGTCACCGAGTTACTGCAAGAGTTCACAATCATCTGATGTTGTCCCATATAAAACATTGCCAAATATCTGTATACCCAGTCCCTGCGGTTCTGATCAGGTTTCAAGCCCCAACTGTAAATGTGCATATCCATATGTGGGTACTCTAATCTTTAGAGCTCCTACCTTCTCAGACTTGCAAACCTCAACTGGCTACAAAGAGCTTGAGAAGAATCTCACTGATTCTTTCTTGTCCCATAAACTTAGAGTCGATTCAGTCGCTTTGAGTAACCTCACCAAGGATTCGCATGCATACCTTAGTATGAGCCTAGAAGTATTCCCACAAAGCCAAGATCGTTTCAATAGAAAGGAAGTTTCTATTATTGCTTTTCTACTCAGTAACCAAACATTCAAGCCTCCAAAATCTTTTGGACCATTCTATTTCATTGGTGATGATTATCAACACTTTGCAGGAGAAACGGAACAATCAGAATCAAACAAATCGTCTAATGTGGGCATTATTATTGGAGCAGCAGCTGGTGGTTCTGTTCTTGTGCTAATATTACTCCTTGTAGGAATTTATGCTATTGGCCAGAAGAGAAGGGCAAAAAGGGCCACTGATCAAAACAATCCTTTTGGCAAATGGGATGTGAATAATAAGACCAGAGGTAGCACTCCCCAGTTAAAGGGGGCTAGATCGTTCTCTTTTGAAGAGCTCATGAAATACAGCAACAACTTTTCAGAAGCAAATGATGTTGGATCTGGGGGCTATGGGAAGGTTTACCGTGGAACTCTTCCCACAGGGCAACTGATTGCCATCAAACGAGCTCACAAAGAATCTATCCAAGGTCGAAATGAGTTCAAAACTGAGATTGAACTTCTTTCACTGGTTCATCATAAGCACCTTGTCAGCCTTGTAGGATTTTGTTTTGACCGAGGTGAGCAGATGCTGGTGTATGAGTATGTAGCAAATGGGACTCTTAAGGATAGTCTTTCAGGTAAGTCAGGAATCAGATTAGATTGGCAGAGGAGACTTAAAATAGCACTTGGTGCAGCTAGAGGCTTAGCCTATCTTCATGAACTTTGCGACCCTCCAATTATACACAGAGATATAAAATCAACTAATGTTCTTTTGGACAACCGCTTGAATGCAAAAGTTGCTGATTTTGGTCTCTCCAAGTCCATGGGTAATAGTGGAAGGGATCATGTCTCCACTCAAGTTAAAGGGACAATGGGATACATGGATCCTGAATATTACATGACCCAGCGATTGACTGAGAAGAGTGATGTTTATGGTTTTGGAGTGCTAATGTTGGAGCTACTTACTGCAAGGAAACCGATAGAACAAGGAAAATATATCGTGCGAGAAGTAAGGATGGCAATGGATAAGACTAAGGATTTATACAACCTTCATGTAATCCTGGATTCAGCCATTGGTTTGGGAACGACACTCAAAGGTTTAGAGAGGTTTGTGGATCTTGCAATGACGTGTGTTGAAGAACAAGGAACCAACAGGCCTACTATGAGTGAGGTGGTAAAAGAGATTGAAAACATTATGCAGCTTGCTGGTCTGAACCCTAATGCTGAGTCGGCATCCACTTCCGCAAGCTATGAGGAGGCAAGCTCTAAGCATCCTTACAGCAATGAGGCCTTTGATTATAGTGGAGTATTTCCAGCTTCAAAGATAGAGCCGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 Pkinase Pkinase_Tyr

Protein Analysis

954

Amino Acids

104.58

Weight (kDa)

6.0

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 62 - 168 7e-07 Leucine-rich repeat region
Pkinase PF00069 637 - 902 1.1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 639 - 903 2.7e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49760 AT5G49770
fragaria_vesca FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23881 FvH4_3g23881 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_5g27161 FvH4_5g27170
malus_domestica MD11G1206300.v1.1
prunus_persica Prupe.4G230700_v2.0.a1 Prupe.4G230700_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1
pyrus_communis pycom11g17930
rosa_chinensis RchiOBHm_Chr5g0042211 RchiOBHm_Chr5g0042261 RchiOBHm_Chr5g0042271 RchiOBHm_Chr5g0042311 RchiOBHm_Chr5g0042381 RchiOBHm_Chr7g0218261
rosa_laevigata RLG00000002384 RLG00000002385 RLG00000034112 RLG00000034114 RLG00000034116 RLG00000034118
rosa_multiflora Rmu_sc0001715.1_g000008 Rmu_sc0001715.1_g000012 Rmu_sc0001715.1_g000026 Rmu_sc0004956.1_g000008
rosa_roxburghii Rroxscaffold_1G00038360 Rroxscaffold_1G00038390 Rroxscaffold_1G00038400 Rroxscaffold_1G00038450 Rroxscaffold_3G00241180
rosa_rugosa Rorug05G0198800 Rorug05G0199000 Rorug05G0199100.1 Rorug05G0199200 Rorug05G0199300 Rorug07G0175900
rosa_samantha Rh5AG283400 Rh5AG283900 Rh5AG284100 Rh5AG284300 Rh5AG284900 Rh5AG285300 Rh5AG285700 Rh5BG289300 Rh5BG289900 Rh5BG290200 Rh5BG290400 Rh5CG320700 Rh5CG321200 Rh5CG321500 Rh5CG322100 Rh5CG322400 Rh5DG298600 Rh5DG299100 Rh5DG299400 Rh5DG300200 Rh7AG317500 Rh7BG308900 Rh7CG334400 Rh7DG316500
rosa_wichuraiana Rw0G001480 Rw0G001500 Rw0G001750 Rw0G001780 Rw0G015430 Rw5G026520 Rw7G026960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 994
AccB1I GGYRCC 1 cut(s) 1049
AccBSI CCGCTC 1 cut(s) 831
AccI GTMKAC 1 cut(s) 1261
AciI CCGC 6 cut(s) 369, 831, 1275, 2308, 2769, 2858
AcoI YGGCCR 1 cut(s) 1756
AcsI RAATTY 5 cut(s) 12, 223, 433, 1105, 1743
AdeI CACNNNGTG 1 cut(s) 2216
AfaI GTAC 2 cut(s) 1147, 1330
AfiI CCNNNNNNNGG 5 cut(s) 317, 398, 1325, 1465, 1852
AflII CTTAAG 1 cut(s) 2147
AflIII ACRYGT 4 cut(s) 658, 750, 900, 2654
AjiI CACGTC 2 cut(s) 901, 2655
AjnI CCWGG 3 cut(s) 316, 553, 2586
Alw21I GWGCWC 4 cut(s) 666, 1348, 1884, 1996
Alw26I GTCTC 6 cut(s) 62, 205, 432, 2191, 2342, 2380
AlwNI CAGNNNCTG 3 cut(s) 1272, 1703, 2116
AoxI GGCC 8 cut(s) 298, 613, 761, 832, 1756, 1779, 2681, 2814
ApeKI GCWGC 6 cut(s) 690, 989, 1697, 1700, 2219, 2725
ApoI RAATTY 5 cut(s) 12, 223, 433, 1105, 1743
ArsI GACNNNNNNTTYG 2 cut(s) 1655, 1687
AseI ATTAAT 1 cut(s) 950
AsuHPI GGTGA 5 cut(s) 560, 1185, 1453, 1628, 2117
AsuII TTCGAA 1 cut(s) 669
AvaII GGWCC 7 cut(s) 117, 308, 380, 402, 524, 1024, 1598
AxyI CCTNAGG 1 cut(s) 1028
BaeGI GKGCMC 1 cut(s) 112
BaeI ACNNNNGTAYC 2 cut(s) 1129, 1162
BalI TGGCCA 1 cut(s) 1758
BamHI GGATCC 2 cut(s) 148, 2410
BanI GGYRCC 1 cut(s) 1049
BanII GRGCYC 3 cut(s) 1348, 1884, 1996
Bbv12I GWGCWC 4 cut(s) 666, 1348, 1884, 1996
BbvI GCAGC 6 cut(s) 702, 976, 1709, 1712, 2231, 2737
BciT130I CCWGG 3 cut(s) 318, 555, 2588
BciVI GTATCC 2 cut(s) 1130, 2396
BclI TGATCA 2 cut(s) 1282, 1786
BcoDI GTCTC 6 cut(s) 62, 205, 432, 2191, 2342, 2380
BfaI CTAG 5 cut(s) 503, 932, 1496, 1860, 2223
BfmI CTRYAG 4 cut(s) 269, 396, 407, 1013
BfrI CTTAAG 1 cut(s) 2147
BfuAI ACCTGC 1 cut(s) 994
BfuI GTATCC 2 cut(s) 1130, 2396
BisI GCNGC 9 cut(s) 370, 691, 832, 990, 1698, 1701, 2220, 2726, 2858
BlpI GCTNAGC 2 cut(s) 827, 2230
BlsI GCNGC 9 cut(s) 371, 692, 833, 991, 1699, 1702, 2221, 2727, 2859
Bme1390I CCNGG 3 cut(s) 318, 555, 2588
Bme18I GGWCC 7 cut(s) 117, 308, 380, 402, 524, 1024, 1598
BmgBI CACGTC 2 cut(s) 901, 2655
BmiI GGNNCC 8 cut(s) 150, 382, 403, 404, 526, 1051, 2412, 2674
BmrFI CCNGG 3 cut(s) 318, 555, 2588
BmrI ACTGGG 4 cut(s) 527, 768, 1260, 1840
BmsI GCATC 5 cut(s) 465, 702, 2103, 2768, 2806
BmuI ACTGGG 4 cut(s) 527, 768, 1260, 1840
BoxI GACNNNNGTC 1 cut(s) 1022
BplI GAGNNNNNCTC 2 cut(s) 2774, 2806
BpmI CTGGAG 2 cut(s) 264, 1206
Bpu1102I GCTNAGC 2 cut(s) 827, 2230
Bpu14I TTCGAA 1 cut(s) 669
BpuEI CTTGAG 2 cut(s) 1412, 2367
BsaBI GATNNNNATC 2 cut(s) 1623, 1982
BsaI GGTCTC 2 cut(s) 205, 2342
Bsc4I CCNNNNNNNGG 5 cut(s) 317, 398, 1325, 1465, 1852
Bse21I CCTNAGG 1 cut(s) 1028
Bse3DI GCAATG 5 cut(s) 574, 1244, 2553, 2655, 2815
Bse8I GATNNNNATC 2 cut(s) 1623, 1982
BseBI CCWGG 3 cut(s) 318, 555, 2588
BseGI GGATG 9 cut(s) 88, 175, 456, 528, 693, 1819, 2547, 2759, 2797
BseJI GATNNNNATC 2 cut(s) 1623, 1982
BseLI CCNNNNNNNGG 5 cut(s) 317, 398, 1325, 1465, 1852
BseMI GCAATG 5 cut(s) 574, 1244, 2553, 2655, 2815
BseRI GAGGAG 2 cut(s) 2209, 2795
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 6 cut(s) 702, 976, 1709, 1712, 2231, 2737
BseYI CCCAGC 1 cut(s) 2430
BsgI GTGCAG 1 cut(s) 2238
BshFI GGCC 8 cut(s) 300, 615, 763, 834, 1758, 1781, 2683, 2816
BshNI GGYRCC 1 cut(s) 1049
BsiHKAI GWGCWC 4 cut(s) 666, 1348, 1884, 1996
BslFI GGGAC 7 cut(s) 366, 388, 1217, 1254, 1405, 2155, 2407
BslI CCNNNNNNNGG 5 cut(s) 317, 398, 1325, 1465, 1852
BsmAI GTCTC 6 cut(s) 62, 205, 432, 2191, 2342, 2380
BsmFI GGGAC 7 cut(s) 366, 388, 1217, 1254, 1405, 2155, 2407
BsmI GAATGC 1 cut(s) 2320
BsnI GGCC 8 cut(s) 300, 615, 763, 834, 1758, 1781, 2683, 2816
Bso31I GGTCTC 2 cut(s) 205, 2342
Bsp119I TTCGAA 1 cut(s) 669
Bsp1286I GDGCHC 5 cut(s) 112, 666, 1348, 1884, 1996
Bsp1720I GCTNAGC 2 cut(s) 827, 2230
Bsp19I CCATGG 3 cut(s) 917, 1141, 2349
BspACI CCGC 6 cut(s) 369, 831, 1275, 2308, 2769, 2858
BspANI GGCC 8 cut(s) 300, 615, 763, 834, 1758, 1781, 2683, 2816
BspHI TCATGA 2 cut(s) 1884, 2242
BspLI GGNNCC 8 cut(s) 150, 382, 403, 404, 526, 1051, 2412, 2674
BspMI ACCTGC 1 cut(s) 994
BspQI GCTCTTC 2 cut(s) 671, 1872
BspT104I TTCGAA 1 cut(s) 669
BspT107I GGYRCC 1 cut(s) 1049
BspTI CTTAAG 1 cut(s) 2147
BspTNI GGTCTC 2 cut(s) 205, 2342
BsrBI CCGCTC 1 cut(s) 831
BsrDI GCAATG 5 cut(s) 574, 1244, 2553, 2655, 2815
BssNAI GTATAC 1 cut(s) 1262
BssT1I CCWWGG 7 cut(s) 706, 917, 1053, 1141, 1464, 2011, 2349
Bst1107I GTATAC 1 cut(s) 1262
Bst2UI CCWGG 3 cut(s) 318, 555, 2588
Bst4CI ACNGT 5 cut(s) 258, 785, 1021, 1304, 1952
Bst6I CTCTTC 5 cut(s) 671, 1757, 1872, 1965, 2441
BstAFI CTTAAG 1 cut(s) 2147
BstAPI GCANNNNNTGC 2 cut(s) 2216, 2324
BstBI TTCGAA 1 cut(s) 669
BstC8I GCNNGC 5 cut(s) 581, 1476, 2730, 2773, 2788
BstDSI CCRYGG 4 cut(s) 917, 1141, 1951, 2349
BstENI CCTNNNNNAGG 2 cut(s) 396, 1463
BstF5I GGATG 9 cut(s) 88, 175, 456, 528, 693, 1819, 2547, 2759, 2797
BstMAI GTCTC 6 cut(s) 62, 205, 432, 2191, 2342, 2380
BstMWI GCNNNNNNNGC 5 cut(s) 1071, 1778, 2216, 2324, 2756
BstNI CCWGG 3 cut(s) 318, 555, 2588
BstNSI RCATGY 5 cut(s) 193, 568, 662, 754, 1478
BstPAI GACNNNNGTC 1 cut(s) 1022
BstSCI CCNGG 3 cut(s) 316, 553, 2586
BstSFI CTRYAG 4 cut(s) 269, 396, 407, 1013
BstSLI GKGCMC 1 cut(s) 112
BstV1I GCAGC 6 cut(s) 702, 976, 1709, 1712, 2231, 2737
BstX2I RGATCY 6 cut(s) 148, 280, 499, 1925, 2410, 2641
BstXI CCANNNNNNTGG 5 cut(s) 138, 521, 1046, 2350, 2605
BstYI RGATCY 6 cut(s) 148, 280, 499, 1925, 2410, 2641
BstZ17I GTATAC 1 cut(s) 1262
Bsu36I CCTNAGG 1 cut(s) 1028
BsuI GTATCC 2 cut(s) 1130, 2396
BsuRI GGCC 8 cut(s) 300, 615, 763, 834, 1758, 1781, 2683, 2816
BtgI CCRYGG 4 cut(s) 917, 1141, 1951, 2349
BtrI CACGTC 2 cut(s) 901, 2655
BtsCI GGATG 9 cut(s) 88, 175, 456, 528, 693, 1819, 2547, 2759, 2797
BtsIMutI CAGTG 4 cut(s) 684, 1404, 1782, 2051
BveI ACCTGC 1 cut(s) 994
Cac8I GCNNGC 5 cut(s) 581, 1476, 2730, 2773, 2788
CaiI CAGNNNCTG 3 cut(s) 1272, 1703, 2116
CciI TCATGA 2 cut(s) 1884, 2242
CseI GACGC 1 cut(s) 1179
CsiI ACCWGGT 1 cut(s) 553
Csp6I GTAC 2 cut(s) 1146, 1329
CviQI GTAC 2 cut(s) 1146, 1329
DraI TTTAAA 2 cut(s) 96, 1008
DraIII CACNNNGTG 1 cut(s) 2216
EaeI YGGCCR 1 cut(s) 1756
Eam1104I CTCTTC 5 cut(s) 671, 1757, 1872, 1965, 2441
EarI CTCTTC 5 cut(s) 671, 1757, 1872, 1965, 2441
Ecl136II GAGCTC 3 cut(s) 1346, 1882, 1994
Eco130I CCWWGG 7 cut(s) 706, 917, 1053, 1141, 1464, 2011, 2349
Eco147I AGGCCT 2 cut(s) 2683, 2816
Eco24I GRGCYC 3 cut(s) 1348, 1884, 1996
Eco31I GGTCTC 2 cut(s) 205, 2342
Eco47I GGWCC 7 cut(s) 117, 308, 380, 402, 524, 1024, 1598
Eco53kI GAGCTC 3 cut(s) 1346, 1882, 1994
Eco81I CCTNAGG 1 cut(s) 1028
EcoICRI GAGCTC 3 cut(s) 1346, 1882, 1994
EcoNI CCTNNNNNAGG 2 cut(s) 396, 1463
EcoO109I RGGNCCY 2 cut(s) 298, 402
EcoRI GAATTC 2 cut(s) 12, 433
EcoRII CCWGG 3 cut(s) 316, 553, 2586
EcoT14I CCWWGG 7 cut(s) 706, 917, 1053, 1141, 1464, 2011, 2349
EcoT22I ATGCAT 1 cut(s) 1480
EcoT38I GRGCYC 3 cut(s) 1348, 1884, 1996
ErhI CCWWGG 7 cut(s) 706, 917, 1053, 1141, 1464, 2011, 2349
FaqI GGGAC 7 cut(s) 366, 388, 1217, 1254, 1405, 2155, 2407
FauNDI CATATG 1 cut(s) 1321
FbaI TGATCA 2 cut(s) 1282, 1786
FblI GTMKAC 1 cut(s) 1261
Fnu4HI GCNGC 9 cut(s) 370, 691, 832, 990, 1698, 1701, 2220, 2726, 2858
FokI GGATG 9 cut(s) 95, 182, 443, 515, 680, 1826, 2554, 2746, 2784
FriOI GRGCYC 3 cut(s) 1348, 1884, 1996
Fsp4HI GCNGC 9 cut(s) 370, 691, 832, 990, 1698, 1701, 2220, 2726, 2858
FspBI CTAG 5 cut(s) 503, 932, 1496, 1860, 2223
GluI GCNGC 9 cut(s) 370, 691, 832, 990, 1698, 1701, 2220, 2726, 2858
GsaI CCCAGC 1 cut(s) 2434
GsuI CTGGAG 2 cut(s) 264, 1206
HaeIII GGCC 8 cut(s) 300, 615, 763, 834, 1758, 1781, 2683, 2816
HgaI GACGC 1 cut(s) 1179
HincII GTYRAC 1 cut(s) 756
HindII GTYRAC 1 cut(s) 756
HindIII AAGCTT 1 cut(s) 629
HpaI GTTAAC 1 cut(s) 756
HphI GGTGA 5 cut(s) 560, 1185, 1453, 1628, 2117
Hpy166II GTNNAC 6 cut(s) 141, 551, 756, 1214, 1262, 1948
Hpy188III TCNNGA 9 cut(s) 80, 146, 243, 560, 1027, 1885, 2172, 2243, 2414
Hpy8I GTNNAC 6 cut(s) 141, 551, 756, 1214, 1262, 1948
Hpy99I CGWCG 1 cut(s) 902
HpyAV CCTTC 6 cut(s) 708, 1363, 1761, 1936, 2358, 2585
HpyCH4III ACNGT 5 cut(s) 258, 785, 1021, 1304, 1952
HpyCH4IV ACGT 3 cut(s) 719, 900, 2654
HpyF10VI GCNNNNNNNGC 5 cut(s) 1071, 1778, 2216, 2324, 2756
HpySE526I ACGT 3 cut(s) 719, 900, 2654
KflI GGGWCCC 1 cut(s) 402
Ksp22I TGATCA 2 cut(s) 1282, 1786
KspAI GTTAAC 1 cut(s) 756
LguI GCTCTTC 2 cut(s) 671, 1872
LmnI GCTCC 7 cut(s) 412, 453, 844, 1187, 1351, 1694, 2482
Lsp1109I GCAGC 6 cut(s) 702, 976, 1709, 1712, 2231, 2737
LweI GCATC 5 cut(s) 465, 702, 2103, 2768, 2806
MabI ACCWGGT 1 cut(s) 553
MaeI CTAG 5 cut(s) 503, 932, 1496, 1860, 2223
MaeII ACGT 3 cut(s) 719, 900, 2654
MaeIII GTNAC 8 cut(s) 85, 182, 715, 1175, 1191, 1199, 1454, 1565
MbiI CCGCTC 1 cut(s) 831
MfeI CAATTG 1 cut(s) 1079
MflI RGATCY 6 cut(s) 148, 280, 499, 1925, 2410, 2641
MhlI GDGCHC 5 cut(s) 112, 666, 1348, 1884, 1996
MlsI TGGCCA 1 cut(s) 1758
MluNI TGGCCA 1 cut(s) 1758
MlyI GAGTC 4 cut(s) 136, 1443, 2137, 2762
MmeI TCCRAC 2 cut(s) 1903, 2460
Mox20I TGGCCA 1 cut(s) 1758
Mph1103I ATGCAT 1 cut(s) 1480
MscI TGGCCA 1 cut(s) 1758
MslI CAYNNNNRTG 4 cut(s) 194, 519, 546, 950
Msp20I TGGCCA 1 cut(s) 1758
MspA1I CMGCKG 2 cut(s) 805, 1703
MspCI CTTAAG 1 cut(s) 2147
MspR9I CCNGG 3 cut(s) 318, 555, 2588
MunI CAATTG 1 cut(s) 1079
Mva1269I GAATGC 1 cut(s) 2320
MvaI CCWGG 3 cut(s) 318, 555, 2588
MwoI GCNNNNNNNGC 5 cut(s) 1071, 1778, 2216, 2324, 2756
NcoI CCATGG 3 cut(s) 917, 1141, 2349
NdeI CATATG 1 cut(s) 1321
NlaIV GGNNCC 8 cut(s) 150, 382, 403, 404, 526, 1051, 2412, 2674
NmuCI GTSAC 3 cut(s) 182, 1175, 1191
NsiI ATGCAT 1 cut(s) 1480
NspI RCATGY 5 cut(s) 193, 568, 662, 754, 1478
NspV TTCGAA 1 cut(s) 669
PacI TTAATTAA 1 cut(s) 1113
PaeI GCATGC 1 cut(s) 1478
PagI TCATGA 2 cut(s) 1884, 2242
PceI AGGCCT 2 cut(s) 2683, 2816
PciI ACATGT 2 cut(s) 658, 750
PciSI GCTCTTC 2 cut(s) 671, 1872
PctI GAATGC 1 cut(s) 2320
PfeI GAWTC 9 cut(s) 859, 1399, 1409, 1439, 1469, 1658, 2003, 2175, 2591
PfoI TCCNGGA 1 cut(s) 2586
PkrI GCNGC 9 cut(s) 371, 692, 833, 991, 1699, 1702, 2221, 2727, 2859
PleI GAGTC 4 cut(s) 136, 1442, 2137, 2761
PpsI GAGTC 4 cut(s) 136, 1442, 2137, 2761
PpuMI RGGWCCY 1 cut(s) 402
PscI ACATGT 2 cut(s) 658, 750
PshAI GACNNNNGTC 1 cut(s) 1022
PshBI ATTAAT 1 cut(s) 950
Psp124BI GAGCTC 3 cut(s) 1348, 1884, 1996
Psp5II RGGWCCY 1 cut(s) 402
Psp6I CCWGG 3 cut(s) 316, 553, 2586
PspFI CCCAGC 1 cut(s) 2430
PspGI CCWGG 3 cut(s) 316, 553, 2586
PspN4I GGNNCC 8 cut(s) 150, 382, 403, 404, 526, 1051, 2412, 2674
PspPPI RGGWCCY 1 cut(s) 402
PstNI CAGNNNCTG 3 cut(s) 1272, 1703, 2116
PsuI RGATCY 6 cut(s) 148, 280, 499, 1925, 2410, 2641
PvuII CAGCTG 2 cut(s) 805, 1703
RsaI GTAC 2 cut(s) 1147, 1330
RsaNI GTAC 2 cut(s) 1146, 1329
RseI CAYNNNNRTG 4 cut(s) 194, 519, 546, 950
SacI GAGCTC 3 cut(s) 1348, 1884, 1996
SapI GCTCTTC 2 cut(s) 671, 1872
SatI GCNGC 9 cut(s) 370, 691, 832, 990, 1698, 1701, 2220, 2726, 2858
SchI GAGTC 4 cut(s) 136, 1443, 2137, 2762
ScrFI CCNGG 3 cut(s) 318, 555, 2588
SduI GDGCHC 5 cut(s) 112, 666, 1348, 1884, 1996
SexAI ACCWGGT 1 cut(s) 553
SfaNI GCATC 5 cut(s) 465, 702, 2103, 2768, 2806
SfcI CTRYAG 4 cut(s) 269, 396, 407, 1013
SfuI TTCGAA 1 cut(s) 669
SinI GGWCC 7 cut(s) 117, 308, 380, 402, 524, 1024, 1598
SmiMI CAYNNNNRTG 4 cut(s) 194, 519, 546, 950
SmlI CTYRAG 3 cut(s) 1391, 2147, 2382
SmoI CTYRAG 3 cut(s) 1391, 2147, 2382
SphI GCATGC 1 cut(s) 1478
SseBI AGGCCT 2 cut(s) 2683, 2816
SsiI CCGC 6 cut(s) 369, 831, 1275, 2308, 2769, 2858
SspI AATATT 3 cut(s) 420, 1728, 2420
SspMI CTAG 5 cut(s) 503, 932, 1496, 1860, 2223
SstI GAGCTC 3 cut(s) 1348, 1884, 1996
StuI AGGCCT 2 cut(s) 2683, 2816
StyD4I CCNGG 3 cut(s) 316, 553, 2586
StyI CCWWGG 7 cut(s) 706, 917, 1053, 1141, 1464, 2011, 2349
TaaI ACNGT 5 cut(s) 258, 785, 1021, 1304, 1952
TaiI ACGT 3 cut(s) 722, 903, 2657
TaqI TCGA 4 cut(s) 669, 897, 1437, 2017
TaqII GACCGA 1 cut(s) 2115
TauI GCSGC 3 cut(s) 372, 834, 2860
TfiI GAWTC 9 cut(s) 859, 1399, 1409, 1439, 1469, 1658, 2003, 2175, 2591
TscAI CASTG 4 cut(s) 691, 1411, 1789, 2058
TseFI GTSAC 3 cut(s) 182, 1175, 1191
TseI GCWGC 6 cut(s) 690, 989, 1697, 1700, 2219, 2725
Tsp45I GTSAC 3 cut(s) 182, 1175, 1191
TspGWI ACGGA 2 cut(s) 456, 1661
TspRI CASTG 4 cut(s) 691, 1411, 1789, 2058
Vha464I CTTAAG 1 cut(s) 2147
VpaK11BI GGWCC 7 cut(s) 117, 308, 380, 402, 524, 1024, 1598
VspI ATTAAT 1 cut(s) 950
XagI CCTNNNNNAGG 2 cut(s) 396, 1463
XapI RAATTY 5 cut(s) 12, 223, 433, 1105, 1743
XceI RCATGY 5 cut(s) 193, 568, 662, 754, 1478
XcmI CCANNNNNNNNNTGG 1 cut(s) 952
XmiI GTMKAC 1 cut(s) 1261
XspI CTAG 5 cut(s) 503, 932, 1496, 1860, 2223
Zsp2I ATGCAT 1 cut(s) 1480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.