Rh5AG284100

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000487
Physical Location & Seq
Reverse (-)
38524979 .. 38532675
7697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG284100.1

Sequence Viewer

Length: 2628 bp
ATGTCTGAAGACAAAGTGAAGTTCAGGAACACTGGGCCAGTTCACCCTCTCACCCGGCAGCCAGTGGCAGACAGGAAGAGATTTGGTGGGGTAAAGTTTGGTGAGATGGAGCGCGACTGCCTTATAGCCCATGGAGCATCAGCAAACTTACATGAACGCCTTTTCACTCTTAGTGACTCCTCCGAAATGCATGCTTGCCTGAAATGCAAAAATGCGGCTAATGTGATAGATGGAACAGTTGATGGCAGTAGAATCAGGGGTCCATATTGCCGGGTCTATAATGATATTATGACACTCAGGGATGAATGGGGCATCAACCCTCCAAGTTGGGTTGGTTCAGATCCTTGTGGCGACTGGGAAGGTATTACTTGCACCAATTCACAGATCACTGCTATAACATTGGCAGACATGGGTTTGAAAGGCGGGCTACCTAGTGATATCGAGACATTATCTGAGCTACAGATTCTGAATCTGGCTTTCAATGAGAACCTGAAGGGACAACTTCCAGCATCAATTGGAAACTTGACCAAACTAAACACCGTAATCCTGGATGGTTGCAGATTCTCTGGTCCTATTCCAGCTACAATAGGATCTTTAAAGCAACTTTCTTATCTATCTCTGAAAAACAATAGATTTAGTGGTCCAATTCCACCTTCCATTGGCAATCTAGCCAATCTTGTCTTTCTGGACCTATCGGACAACAGGCTTGGGGATTCCATTCCAGTCTCTAATGGGACTGCACCTGGTCTTGATATGTTAAATAAAACAAAGCACTTTCATTTTTCAAATAACCAGCTCTCGGGCAGCATTCCACCTCAACTGTTCAGCTCAAATATGCCTCTGATACATTTGATTTTTGACAGAAATAATTTTACTGGCAGCATCCCTTCCACACTTGGACTTGTGAAAACTCTGGAGGCAGTACGCCTTGATAGAAATTCATTAAGTGGGTCTGTACCTACGAGCCTCAGAAATCTTACAAATGTGTCTGAACTGCACTTGTCCAACAATAAGCTGACTGGCCCGGTGCCCAACCTTACTCTCATGGATTCCCTCAACTATGTGGATATGAGCAATAATACTTTTGATGCGTCAGATATTCCAAATTGGTTTTCAACCTTGAAGTTCTTGACAACATTAATGATGGAAAACACAGGACTTCAGGGACAAGTCCCTCAAGCCCTTTTCAGCCTTCAAAATTTGGAGACAGTGATACTGAAGAACAACAGTCTCAATGGTACATTGGATATTGGAACAAATTATGGCGACCAATTGAACCTAATTGATCTGCAGAAGAACAACATTGCTAATCTTGCACCAATTGATGAGGGATCCAACTACACATTGATTCTAGTTGACAATCCAATTTGTGAGGGGAGTCAGAATAACATGGCAAAGAACTACTGCAATGTTTCTCCTCCTAATTCCTCAATTTCAACTACACCAAGTAATTGTGCTGCCTGTAGTTCCGGCCAGGTTGCTTCCCCCAACTGTAAATGTGCATCTCCCTATACACGGACTCTAGTCTTCTTATTTGTTTCTTTCTCAAACTTGGATGACGTCAATTATTACAAAGCTCTCTCAGACAATCTCACAAGATCTTTTCAGTCCAAAAACCTTCCTGTGGATTCAGTTTCACTGAGTCATCCGAGCTGGAGCTCATCTTATCAACTTGAAATTATCATAGAAATTTTCCCATCCGGTGATCAAGTTAGCTTCAACCAAACAGAAAGTTCAGCGATTACATCTGTTATTAGCAACCAGACTCTAGAGGGACGTCCAAATTCTTTTGGACCCTACTCTTTTCTTGTTCTTTACACCAACTCTGGAGGATCAAACAAGGCCGTGATAATTGGAACGGCAATTGGTGGTTCTGTGCTCCTTTCATTATTAGTCCTTGTAGGGCTTTATGCTCTCCACCAGAAGAGAAAAGCAGATGAGTCATTGAGTGGATCGATCCTTTTGCAGACAGGGATTTCAAATAGCAGTGCTAGTGGTCCTCAACTAAAAGGAGCAAGATTGTTCTCATTTGAAGAGCTCAAGAAATGCACCGATGGTTTTTCGGAAGCAAATGATATTGGATCTGGGGGTTATGGCCAGGTGTATAAGGGAATTCTTCCAACAGGCCAAATGGCTGCCATTAAAAGAGCTAAACGGGAATCTATGCAGGGAGGAGTTGAGTTCAAAGCCGAGGTTGAGCTCCTATCACGAGTACATCATAAGAATCTTGTTGGCCTTGTAGGTTTTTGTTTTGAGCAAGGGGAACAAATATTGGTATATGAGTATGTTCCAAATGGTGATTTGAGGGACAGCCTTTCAGGGAAGTCCGGAATCAGGTTGGACTGGACAAGAAGACTTCAAGTAGCACTTGGTGCAGCGAGAGGAACAGAGCTGAAAGGTGTAGAAATGTTTGTGGATTTGGCAATGTCTTGTGTAGAAGAATTAAGGGATAAAAGACCAAGAATGGGGGAAGTGGTGAAACAGATAGAGAACATTATACAAATTGCTGCTTTGAACGCCGGTACTAATCCATTGTCAACTTCAGCAAGTTATGAGGAATATGAGTGTAAGGACAGCACCGAGGATGTTTACAGCAGACCCTTTGATTATAGTGGAAAGGGTTTTTGA

Protein Analysis

875

Amino Acids

94.98

Weight (kDa)

5.62

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_7 PF04560 29 - 86 7.5e-15 RNA polymerase Rpb2, domain 7
LRR_14 PF23598 142 - 208 1.7e-06 Leucine-rich repeat region
LRR_8 PF13855 177 - 236 1e-05 Leucine rich repeat
LRR_14 PF23598 194 - 422 2e-09 Leucine-rich repeat region
Pkinase PF00069 688 - 778 4.6e-13 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 691 - 796 8.6e-16 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49760 AT5G49770
fragaria_vesca FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23881 FvH4_3g23881 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_5g27161 FvH4_5g27170
malus_domestica MD11G1206300.v1.1
prunus_persica Prupe.4G230700_v2.0.a1 Prupe.4G230700_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1
pyrus_communis pycom11g17930
rosa_chinensis RchiOBHm_Chr5g0042211 RchiOBHm_Chr5g0042261 RchiOBHm_Chr5g0042271 RchiOBHm_Chr5g0042311 RchiOBHm_Chr5g0042381 RchiOBHm_Chr7g0218261
rosa_laevigata RLG00000002384 RLG00000002385 RLG00000034112 RLG00000034114 RLG00000034116 RLG00000034118
rosa_multiflora Rmu_sc0001715.1_g000008 Rmu_sc0001715.1_g000012 Rmu_sc0001715.1_g000026 Rmu_sc0004956.1_g000008
rosa_roxburghii Rroxscaffold_1G00038360 Rroxscaffold_1G00038390 Rroxscaffold_1G00038400 Rroxscaffold_1G00038450 Rroxscaffold_3G00241180
rosa_rugosa Rorug05G0198800 Rorug05G0199000 Rorug05G0199100.1 Rorug05G0199200 Rorug05G0199300 Rorug07G0175900
rosa_samantha Rh5AG283400 Rh5AG283900 Rh5AG284100 Rh5AG284300 Rh5AG284900 Rh5AG285300 Rh5AG285700 Rh5BG289300 Rh5BG289900 Rh5BG290200 Rh5BG290400 Rh5CG320700 Rh5CG321200 Rh5CG321500 Rh5CG322100 Rh5CG322400 Rh5DG298600 Rh5DG299100 Rh5DG299400 Rh5DG300200 Rh7AG317500 Rh7BG308900 Rh7CG334400 Rh7DG316500
rosa_wichuraiana Rw0G001480 Rw0G001500 Rw0G001750 Rw0G001780 Rw0G015430 Rw5G026520 Rw7G026960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1563, 1780
AccB1I GGYRCC 1 cut(s) 1027
AccII CGCG 1 cut(s) 114
AccIII TCCGGA 1 cut(s) 2327
AciI CCGC 2 cut(s) 215, 423
AclWI GGATC 8 cut(s) 335, 598, 1326, 1339, 1840, 1951, 1960, 2089
AcoI YGGCCR 2 cut(s) 1471, 2095
AcsI RAATTY 5 cut(s) 937, 1198, 1689, 1783, 2112
AcuI CTGAAG 5 cut(s) 27, 512, 1145, 1238, 2526
AcyI GRCGYC 2 cut(s) 1560, 1777
AdeI CACNNNGTG 1 cut(s) 2372
AfaI GTAC 5 cut(s) 924, 957, 1240, 2214, 2524
AfiI CCNNNNNNNGG 6 cut(s) 659, 799, 1515, 1624, 2334, 2465
AjnI CCWGG 4 cut(s) 546, 742, 1473, 2097
AjuI GAANNNNNNNTTGG 4 cut(s) 637, 669, 1096, 1128
Alw21I GWGCWC 4 cut(s) 1661, 1881, 2040, 2202
Alw26I GTCTC 4 cut(s) 437, 730, 1199, 1235
AlwI GGATC 8 cut(s) 335, 598, 1326, 1339, 1840, 1951, 1960, 2089
AlwNI CAGNNNCTG 1 cut(s) 466
Ama87I CYCGRG 1 cut(s) 799
Aor13HI TCCGGA 1 cut(s) 2327
AoxI GGCC 7 cut(s) 35, 1021, 1471, 1842, 2095, 2125, 2233
ApeKI GCWGC 7 cut(s) 58, 804, 879, 1457, 2135, 2375, 2507
ApoI RAATTY 5 cut(s) 937, 1198, 1689, 1783, 2112
AseI ATTAAT 1 cut(s) 1139
AspLEI GCGC 1 cut(s) 114
AspS9I GGNCC 8 cut(s) 35, 260, 569, 641, 688, 1022, 1793, 1997
AsuC2I CCSGG 3 cut(s) 55, 272, 1025
AsuHPI GGTGA 6 cut(s) 35, 43, 113, 1715, 2309, 2488
AvaI CYCGRG 1 cut(s) 799
AvaII GGWCC 6 cut(s) 260, 569, 641, 688, 1793, 1997
BaeGI GKGCMC 1 cut(s) 1032
BalI TGGCCA 1 cut(s) 2097
BamHI GGATCC 1 cut(s) 1331
BanI GGYRCC 1 cut(s) 1027
BanII GRGCYC 3 cut(s) 1661, 2040, 2202
BauI CACGAG 1 cut(s) 2208
BbsI GAAGAC 3 cut(s) 15, 1519, 2359
Bbv12I GWGCWC 4 cut(s) 1661, 1881, 2040, 2202
BbvI GCAGC 7 cut(s) 70, 816, 891, 1444, 2122, 2387, 2494
BccI CCATC 7 cut(s) 100, 224, 236, 545, 1138, 1705, 2048
BceAI ACGGC 2 cut(s) 1829, 1875
BcgI CGANNNNNNTGC 4 cut(s) 173, 207, 1945, 1979
BciT130I CCWGG 4 cut(s) 548, 744, 1475, 2099
BclI TGATCA 1 cut(s) 1705
BcnI CCSGG 3 cut(s) 55, 272, 1025
BcoDI GTCTC 4 cut(s) 437, 730, 1199, 1235
BfaI CTAG 6 cut(s) 432, 668, 1352, 1523, 1769, 1992
BfmI CTRYAG 3 cut(s) 458, 1289, 1462
BglII AGATCT 1 cut(s) 1598
BisI GCNGC 8 cut(s) 59, 216, 805, 880, 1458, 2136, 2376, 2508
BlsI GCNGC 8 cut(s) 60, 217, 806, 881, 1459, 2137, 2377, 2509
Bme1390I CCNGG 7 cut(s) 55, 272, 548, 744, 1025, 1475, 2099
Bme18I GGWCC 6 cut(s) 260, 569, 641, 688, 1793, 1997
BmeT110I CYCGRG 1 cut(s) 799
BmgT120I GGNCC 8 cut(s) 35, 260, 569, 641, 688, 1022, 1793, 1997
BmiI GGNNCC 4 cut(s) 261, 1029, 1333, 1795
BmrFI CCNGG 7 cut(s) 55, 272, 548, 744, 1025, 1475, 2099
BmrI ACTGGG 2 cut(s) 42, 364
BmsI GCATC 6 cut(s) 146, 321, 518, 891, 1078, 1511
BmuI ACTGGG 2 cut(s) 42, 364
BoxI GACNNNNGTC 1 cut(s) 1523
BpiI GAAGAC 3 cut(s) 15, 1519, 2359
BpmI CTGGAG 3 cut(s) 935, 1675, 1848
BpuEI CTTGAG 2 cut(s) 1161, 2024
BpuMI CCSGG 3 cut(s) 55, 272, 1025
Bsa29I ATCGAT 1 cut(s) 1955
BsaHI GRCGYC 2 cut(s) 1560, 1777
BsaJI CCNNGG 3 cut(s) 130, 2190, 2580
BsaWI WCCGGW 2 cut(s) 1700, 2327
Bsc4I CCNNNNNNNGG 6 cut(s) 659, 799, 1515, 1624, 2334, 2465
Bse118I RCCGGY 1 cut(s) 2519
Bse1I ACTGG 8 cut(s) 37, 38, 62, 359, 722, 880, 1024, 2348
Bse3DI GCAATG 3 cut(s) 1302, 1414, 2430
BseAI TCCGGA 1 cut(s) 2327
BseBI CCWGG 4 cut(s) 548, 744, 1475, 2099
BseCI ATCGAT 1 cut(s) 1955
BseDI CCNNGG 3 cut(s) 130, 2190, 2580
BseGI GGATG 7 cut(s) 307, 556, 882, 1561, 1645, 1697, 2590
BseLI CCNNNNNNNGG 6 cut(s) 659, 799, 1515, 1624, 2334, 2465
BseMI GCAATG 3 cut(s) 1302, 1414, 2430
BseMII CTCAG 5 cut(s) 310, 444, 982, 1596, 1631
BseNI ACTGG 8 cut(s) 37, 38, 62, 359, 722, 880, 1024, 2348
BseRI GAGGAG 3 cut(s) 169, 1407, 2187
BseSI GKGCMC 1 cut(s) 1032
BseXI GCAGC 7 cut(s) 70, 816, 891, 1444, 2122, 2387, 2494
BsgI GTGCAG 3 cut(s) 723, 980, 2394
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 7 cut(s) 37, 1023, 1473, 1844, 2097, 2127, 2235
BshNI GGYRCC 1 cut(s) 1027
BshVI ATCGAT 1 cut(s) 1955
BsiHKAI GWGCWC 4 cut(s) 1661, 1881, 2040, 2202
BsiHKCI CYCGRG 1 cut(s) 799
BsiSI CCGG 7 cut(s) 55, 271, 1025, 1470, 1701, 2328, 2520
BslFI GGGAC 6 cut(s) 510, 748, 1157, 1179, 1788, 2321
BslI CCNNNNNNNGG 6 cut(s) 659, 799, 1515, 1624, 2334, 2465
BsmAI GTCTC 4 cut(s) 437, 730, 1199, 1235
BsmFI GGGAC 6 cut(s) 510, 748, 1157, 1179, 1788, 2321
BsmI GAATGC 1 cut(s) 807
BsnI GGCC 7 cut(s) 37, 1023, 1473, 1844, 2097, 2127, 2235
BsoBI CYCGRG 1 cut(s) 799
Bsp1286I GDGCHC 5 cut(s) 1032, 1661, 1881, 2040, 2202
Bsp13I TCCGGA 1 cut(s) 2327
Bsp19I CCATGG 1 cut(s) 130
BspACI CCGC 2 cut(s) 215, 423
BspANI GGCC 7 cut(s) 37, 1023, 1473, 1844, 2097, 2127, 2235
BspCNI CTCAG 5 cut(s) 309, 445, 981, 1595, 1632
BspDI ATCGAT 1 cut(s) 1955
BspEI TCCGGA 1 cut(s) 2327
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 4 cut(s) 261, 1029, 1333, 1795
BspMAI CTGCAG 1 cut(s) 1293
BspPI GGATC 8 cut(s) 335, 598, 1326, 1339, 1840, 1951, 1960, 2089
BspQI GCTCTTC 1 cut(s) 2028
BspT107I GGYRCC 1 cut(s) 1027
BsrDI GCAATG 3 cut(s) 1302, 1414, 2430
BsrFI RCCGGY 1 cut(s) 2519
BsrI ACTGG 8 cut(s) 37, 38, 62, 359, 722, 880, 1024, 2348
BssAI RCCGGY 1 cut(s) 2519
BssECI CCNNGG 3 cut(s) 130, 2190, 2580
BssNI GRCGYC 2 cut(s) 1560, 1777
BssSI CACGAG 1 cut(s) 2208
BssT1I CCWWGG 1 cut(s) 130
Bst2BI CACGAG 1 cut(s) 2208
Bst2UI CCWGG 4 cut(s) 548, 744, 1475, 2099
Bst4CI ACNGT 6 cut(s) 238, 541, 822, 1210, 1229, 1493
Bst6I CTCTTC 3 cut(s) 71, 1919, 2028
BstACI GRCGYC 2 cut(s) 1560, 1777
BstAPI GCANNNNNTGC 1 cut(s) 2372
BstC8I GCNNGC 3 cut(s) 192, 196, 425
BstDEI CTNAG 6 cut(s) 170, 296, 453, 968, 1582, 1640
BstDSI CCRYGG 1 cut(s) 130
BstF5I GGATG 7 cut(s) 307, 556, 882, 1561, 1645, 1697, 2590
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 114
BstMAI GTCTC 4 cut(s) 437, 730, 1199, 1235
BstMWI GCNNNNNNNGC 5 cut(s) 134, 204, 1313, 2372, 2516
BstNI CCWGG 4 cut(s) 548, 744, 1475, 2099
BstNSI RCATGY 1 cut(s) 194
BstPAI GACNNNNGTC 1 cut(s) 1523
BstSCI CCNGG 7 cut(s) 53, 270, 546, 742, 1023, 1473, 2097
BstSFI CTRYAG 3 cut(s) 458, 1289, 1462
BstSLI GKGCMC 1 cut(s) 1032
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 7 cut(s) 70, 816, 891, 1444, 2122, 2387, 2494
BstV2I GAAGAC 3 cut(s) 15, 1519, 2359
BstX2I RGATCY 5 cut(s) 340, 590, 1331, 1598, 2081
BstYI RGATCY 5 cut(s) 340, 590, 1331, 1598, 2081
Bsu15I ATCGAT 1 cut(s) 1955
BsuRI GGCC 7 cut(s) 37, 1023, 1473, 1844, 2097, 2127, 2235
BsuTUI ATCGAT 1 cut(s) 1955
BtgI CCRYGG 1 cut(s) 130
BtsCI GGATG 7 cut(s) 307, 556, 882, 1561, 1645, 1697, 2590
BtsI GCAGTG 2 cut(s) 387, 1993
BtsIMutI CAGTG 6 cut(s) 30, 69, 387, 1215, 1637, 1993
Cac8I GCNNGC 3 cut(s) 192, 196, 425
CaiI CAGNNNCTG 1 cut(s) 466
CfoI GCGC 1 cut(s) 114
Cfr10I RCCGGY 1 cut(s) 2519
Cfr13I GGNCC 8 cut(s) 35, 260, 569, 641, 688, 1022, 1793, 1997
ClaI ATCGAT 1 cut(s) 1955
CseI GACGC 1 cut(s) 1080
CsiI ACCWGGT 1 cut(s) 742
Csp6I GTAC 5 cut(s) 923, 956, 1239, 2213, 2523
CviAII CATG 6 cut(s) 131, 152, 191, 409, 1045, 1390
CviQI GTAC 5 cut(s) 923, 956, 1239, 2213, 2523
DdeI CTNAG 6 cut(s) 170, 296, 453, 968, 1582, 1640
DraI TTTAAA 1 cut(s) 597
DraIII CACNNNGTG 1 cut(s) 2372
EaeI YGGCCR 2 cut(s) 1471, 2095
Eam1104I CTCTTC 3 cut(s) 71, 1919, 2028
EarI CTCTTC 3 cut(s) 71, 1919, 2028
Ecl136II GAGCTC 3 cut(s) 1659, 2038, 2200
Eco130I CCWWGG 1 cut(s) 130
Eco24I GRGCYC 3 cut(s) 1661, 2040, 2202
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 6 cut(s) 260, 569, 641, 688, 1793, 1997
Eco53kI GAGCTC 3 cut(s) 1659, 2038, 2200
Eco57I CTGAAG 5 cut(s) 27, 512, 1145, 1238, 2526
Eco88I CYCGRG 1 cut(s) 799
EcoICRI GAGCTC 3 cut(s) 1659, 2038, 2200
EcoRI GAATTC 1 cut(s) 2112
EcoRII CCWGG 4 cut(s) 546, 742, 1473, 2097
EcoRV GATATC 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 130
EcoT22I ATGCAT 1 cut(s) 192
EcoT38I GRGCYC 3 cut(s) 1661, 2040, 2202
ErhI CCWWGG 1 cut(s) 130
FaeI CATG 6 cut(s) 134, 155, 194, 412, 1048, 1393
FalI AAGNNNNNCTT 2 cut(s) 2352, 2384
FaqI GGGAC 6 cut(s) 510, 748, 1157, 1179, 1788, 2321
FatI CATG 6 cut(s) 130, 151, 190, 408, 1044, 1389
FauI CCCGC 1 cut(s) 416
FbaI TGATCA 1 cut(s) 1705
Fnu4HI GCNGC 8 cut(s) 59, 216, 805, 880, 1458, 2136, 2376, 2508
FokI GGATG 7 cut(s) 314, 563, 869, 1568, 1632, 1684, 2597
FriOI GRGCYC 3 cut(s) 1661, 2040, 2202
Fsp4HI GCNGC 8 cut(s) 59, 216, 805, 880, 1458, 2136, 2376, 2508
FspBI CTAG 6 cut(s) 432, 668, 1352, 1523, 1769, 1992
GlaI GCGC 1 cut(s) 113
GluI GCNGC 8 cut(s) 59, 216, 805, 880, 1458, 2136, 2376, 2508
GsuI CTGGAG 3 cut(s) 935, 1675, 1848
HaeIII GGCC 7 cut(s) 37, 1023, 1473, 1844, 2097, 2127, 2235
HapII CCGG 7 cut(s) 55, 271, 1025, 1470, 1701, 2328, 2520
HgaI GACGC 1 cut(s) 1080
HhaI GCGC 1 cut(s) 114
Hin1I GRCGYC 2 cut(s) 1560, 1777
Hin1II CATG 6 cut(s) 134, 155, 194, 412, 1048, 1393
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HincII GTYRAC 2 cut(s) 1357, 2538
HindII GTYRAC 2 cut(s) 1357, 2538
HpaII CCGG 7 cut(s) 55, 271, 1025, 1470, 1701, 2328, 2520
HphI GGTGA 6 cut(s) 35, 43, 113, 1715, 2309, 2488
Hpy166II GTNNAC 4 cut(s) 43, 1357, 2538, 2590
Hpy8I GTNNAC 4 cut(s) 43, 1357, 2538, 2590
HpyAV CCTTC 6 cut(s) 353, 487, 663, 897, 1202, 1628
HpyCH4III ACNGT 6 cut(s) 238, 541, 822, 1210, 1229, 1493
HpyCH4IV ACGT 2 cut(s) 1560, 1777
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 204, 1313, 2372, 2516
HpyF3I CTNAG 6 cut(s) 170, 296, 453, 968, 1582, 1640
HpySE526I ACGT 2 cut(s) 1560, 1777
Hsp92I GRCGYC 2 cut(s) 1560, 1777
Hsp92II CATG 6 cut(s) 134, 155, 194, 412, 1048, 1393
HspAI GCGC 1 cut(s) 112
Kpn2I TCCGGA 1 cut(s) 2327
Ksp22I TGATCA 1 cut(s) 1705
LguI GCTCTTC 1 cut(s) 2028
LmnI GCTCC 6 cut(s) 109, 134, 1656, 1884, 2012, 2205
Lsp1109I GCAGC 7 cut(s) 70, 816, 891, 1444, 2122, 2387, 2494
LweI GCATC 6 cut(s) 146, 321, 518, 891, 1078, 1511
MabI ACCWGGT 1 cut(s) 742
MaeI CTAG 6 cut(s) 432, 668, 1352, 1523, 1769, 1992
MaeII ACGT 2 cut(s) 1560, 1777
MaeIII GTNAC 1 cut(s) 173
MfeI CAATTG 4 cut(s) 513, 1271, 1320, 1863
MflI RGATCY 5 cut(s) 340, 590, 1331, 1598, 2081
MhlI GDGCHC 5 cut(s) 1032, 1661, 1881, 2040, 2202
MlsI TGGCCA 1 cut(s) 2097
MluNI TGGCCA 1 cut(s) 2097
MlyI GAGTC 6 cut(s) 170, 1387, 1513, 1651, 1759, 1949
MmeI TCCRAC 4 cut(s) 1029, 1359, 2144, 2319
Mox20I TGGCCA 1 cut(s) 2097
Mph1103I ATGCAT 1 cut(s) 192
MroI TCCGGA 1 cut(s) 2327
MscI TGGCCA 1 cut(s) 2097
MseI TTAA 6 cut(s) 596, 758, 944, 1139, 2142, 2444
Msp20I TGGCCA 1 cut(s) 2097
MspI CCGG 7 cut(s) 55, 271, 1025, 1470, 1701, 2328, 2520
MspR9I CCNGG 7 cut(s) 55, 272, 548, 744, 1025, 1475, 2099
MunI CAATTG 4 cut(s) 513, 1271, 1320, 1863
Mva1269I GAATGC 1 cut(s) 807
MvaI CCWGG 4 cut(s) 548, 744, 1475, 2099
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 5 cut(s) 134, 204, 1313, 2372, 2516
NciI CCSGG 3 cut(s) 55, 272, 1025
NcoI CCATGG 1 cut(s) 130
NlaIII CATG 6 cut(s) 134, 155, 194, 412, 1048, 1393
NlaIV GGNNCC 4 cut(s) 261, 1029, 1333, 1795
NmeAIII GCCGAG 1 cut(s) 2215
NmuCI GTSAC 1 cut(s) 173
NsiI ATGCAT 1 cut(s) 192
NspI RCATGY 1 cut(s) 194
PaeI GCATGC 1 cut(s) 194
PciSI GCTCTTC 1 cut(s) 2028
PctI GAATGC 1 cut(s) 807
PfoI TCCNGGA 1 cut(s) 546
PkrI GCNGC 8 cut(s) 60, 217, 806, 881, 1459, 2137, 2377, 2509
PleI GAGTC 6 cut(s) 170, 1386, 1513, 1650, 1759, 1948
PpsI GAGTC 6 cut(s) 170, 1386, 1513, 1650, 1759, 1948
PshAI GACNNNNGTC 1 cut(s) 1523
PshBI ATTAAT 1 cut(s) 1139
Psp124BI GAGCTC 3 cut(s) 1661, 2040, 2202
Psp6I CCWGG 4 cut(s) 546, 742, 1473, 2097
PspGI CCWGG 4 cut(s) 546, 742, 1473, 2097
PspN4I GGNNCC 4 cut(s) 261, 1029, 1333, 1795
PspPI GGNCC 8 cut(s) 35, 260, 569, 641, 688, 1022, 1793, 1997
PstI CTGCAG 1 cut(s) 1293
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 5 cut(s) 340, 590, 1331, 1598, 2081
RsaI GTAC 5 cut(s) 924, 957, 1240, 2214, 2524
RsaNI GTAC 5 cut(s) 923, 956, 1239, 2213, 2523
SacI GAGCTC 3 cut(s) 1661, 2040, 2202
SapI GCTCTTC 1 cut(s) 2028
SaqAI TTAA 6 cut(s) 596, 758, 944, 1139, 2142, 2444
SatI GCNGC 8 cut(s) 59, 216, 805, 880, 1458, 2136, 2376, 2508
Sau96I GGNCC 8 cut(s) 35, 260, 569, 641, 688, 1022, 1793, 1997
SchI GAGTC 6 cut(s) 170, 1387, 1513, 1651, 1759, 1949
ScrFI CCNGG 7 cut(s) 55, 272, 548, 744, 1025, 1475, 2099
SduI GDGCHC 5 cut(s) 1032, 1661, 1881, 2040, 2202
SexAI ACCWGGT 1 cut(s) 742
SfaNI GCATC 6 cut(s) 146, 321, 518, 891, 1078, 1511
SfcI CTRYAG 3 cut(s) 458, 1289, 1462
SinI GGWCC 6 cut(s) 260, 569, 641, 688, 1793, 1997
SmlI CTYRAG 2 cut(s) 1176, 2039
SmoI CTYRAG 2 cut(s) 1176, 2039
SphI GCATGC 1 cut(s) 194
SsiI CCGC 2 cut(s) 215, 423
SspI AATATT 1 cut(s) 2271
SspMI CTAG 6 cut(s) 432, 668, 1352, 1523, 1769, 1992
SstI GAGCTC 3 cut(s) 1661, 2040, 2202
StyD4I CCNGG 7 cut(s) 53, 270, 546, 742, 1023, 1473, 2097
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 6 cut(s) 238, 541, 822, 1210, 1229, 1493
TaiI ACGT 2 cut(s) 1563, 1780
TaqI TCGA 2 cut(s) 441, 1955
TatI WGTACW 1 cut(s) 2212
TauI GCSGC 1 cut(s) 218
Tru1I TTAA 6 cut(s) 596, 758, 944, 1139, 2142, 2444
Tru9I TTAA 6 cut(s) 596, 758, 944, 1139, 2142, 2444
TscAI CASTG 6 cut(s) 37, 69, 394, 1215, 1644, 1993
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 7 cut(s) 58, 804, 879, 1457, 2135, 2375, 2507
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 5 cut(s) 168, 318, 767, 930, 1875
TspGWI ACGGA 1 cut(s) 1531
TspRI CASTG 6 cut(s) 37, 69, 394, 1215, 1644, 1993
VpaK11BI GGWCC 6 cut(s) 260, 569, 641, 688, 1793, 1997
VspI ATTAAT 1 cut(s) 1139
XapI RAATTY 5 cut(s) 937, 1198, 1689, 1783, 2112
XbaI TCTAGA 1 cut(s) 1768
XceI RCATGY 1 cut(s) 194
XspI CTAG 6 cut(s) 432, 668, 1352, 1523, 1769, 1992
ZraI GACGTC 2 cut(s) 1561, 1778
Zsp2I ATGCAT 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.