Rh5AG285300

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000487
Physical Location & Seq
Reverse (-)
38636745 .. 38647099
10355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG285300.1

Sequence Viewer

Length: 3135 bp
ATGGGTCGTGCTCGAGCCATTGGTGCGTTTCTGGTGCTTGTGTTAGTCCAGTTCCTTGTTATTGCAGCAGAGGCAAACACTCAGGAATATGGTTACATGCAGATTCTCATGGATGAGTGGAATATAAAACCTCCTAGTTGGGTGAGTGCCGATCCTTGTGACGGCTGGGAAGGTATTAAGTGCACCAATTCACGCATCACTGCCTTAGTGTTGGCAGACATGGGTTTGAAAGGCCAGCTACCGACTGATATCGAGTTGTTATCTGAGCTACAAATTCTGAATTTATCTTTCAACAAGGGCCTGACGGGACAACTTCGAGCTTCAATTGGAAAATTGACGAAACTAGTACACTTAAACCTGGTTGGTTGCAGTTTCTTTGGTCCTATTCCAGACGCTATAGGATCTCTAACGAAGCTGTCTTACCTATCTCTGAAAAACAATAGTTTCAGTGGGCCAATTCCGCCTTCCATTGGTAATCTGGCCAATCTTACTTTTATAGACCTATCTGACAACAAGCTTCAGGGATCCATCCCAGTCTCTAATGGGACTACACTTGGTCTTGATAAGTTACTTAAGGCGAGACACTTTCATTTTGGGAACAATCAGCTCTCGGGCCCAATTCCACCTCGACTTTTCAGCTCAACGATGGCCATGATACATTTAATTTTTGACAGAAACAATCTAAGTGGTAGCATCCCTTCAACTGTTGGACTTGTTCAGACATTGCTGGCGGTACGCCTGGATGGAAATTCATTAAGTGGGAGTGTGCCTTCGAGCCTCAACAACCTTACTAATGTGTCTGAACTGCACTTGTCCAATAATATGCTGACTGGCCCTATGCCCAACCTTACTGGCATGAATTCCCTCAACTATGTGGATTTGAGCAACAATACTTTTGCTGTGTCGGAGGTTCCTACCTGGTTTTCTACCTTGAAGCTTTTGACAACATTAATGATGGAAAACACGGGGCTTCAGGGAGAAGTTCCTCGTGCCCTCTTCGGCTTTGGGAATTTGGAGACAGTGGTACTGCGGAACAACCTTCTCAGTGGCTTATTGGATGTTAGAAACTCCAGCAACCAGTTGCAACTAATTGATCTGCAGCGGAACTTCATTACTGAACTTGCACCAAACAAGGAAGGATCCAACTACACATTGATACTGGTTAATAATCCAATTTGTGAGGGGAGTCAGACTGATAACTACTGCATTGTTTCTCCAACTAATGTACCAAACGAAATGTCACCAACTCCAAGCAACTGTACACCTGTTGCCTGTCCTTCAGGCCAGGTTTCTTCCCCCAACTGTAAATGTGCATCTCCCTACAGAGGAACTCTAGTCTTCATATTTGTTTCTTTCTCAAATTTGGGAAACTTTAGTTATTACAGATATCTTGAGGACTCTATCCTGAAAAGTTGTCAGTCCTTTAACCTCCCTGTGGATTCAGTTTCTCTGGGTCGTCCTTCCTGGGGTTCATCTTTTCATCTTGAGCTAGTCATAGAAGTCTTCCCATCTGGTGATCAAGTGCGCTTCAACCAAACAGGAGCTTCAGCAATTGCTTCTGTTTTAAGCAACCAGACTCTCGAAGGGCGTCCAAGATTTTTTGGACCCTACTCTGTGTTTTATCCTTCCGGCAGCTCTGGAGGATCAAATAAGGGACTGATAATTGGTGCATCCATTGGTGGTTCTGTGCTTCTGTTATTAATAGTCCTTGTAGGGTTTTATGCTCTCTACCAAAAGAGGAGAGGAGACTCGCCATCACGTGGATCAATCCTCATGACAGGGGTTTCAAATGTCAGTGGTCCACAGTTAAAAGGAGCAAGGTTGTTCTCTTTTGAAGAGCTGAAGAAATATACAAACGGTTTTTCAGAAGAAAATGATATTGGATCTGGGGGTTATGGCCAGGTGTATCGGGGAACTCTTCCTATGGGCCAAACGGTTGCCATTAAAAGAGCTAAACGGGAATCTATGCAAGGTGCGCCTGAGTTCAAGGCCGAGGTTGAGCTTCTATCAAGAGTACACCATAAGAATCTTGTCAGCCTTGTTGGTTTTTGTTTGGACCAAGGTGAACAAATGTTGGTATATGAGTATGTTCAAAATGGTGATCTGCTGGATAGTCTTTTAGGAAAGTCAGGAATCAGTGGAAAGGATTATGTTACTACTCAAGTCAAAGGGACAATGGGCTACTTGGATCCTGAGTATTACATGACTCAACAGTTGACTGAGAAGAGTGATGTATATAGCTTCGGTGTGGTAATGTTGGAGCTGATAACTGCGAGAAGGCCAATAGAGCGAGGGAAGTACATTGTGAGAGTGGTACAGTCGGCAATGGATAAGTCAAAAGATTTGTATAACCTTCATGAAGTACTTGATCCAGCCATTGATTTAGAAAATGATCAGCTGAAAGGTTTAGAAATGTTTGTCAATTTGGCAATGTTGTGTGTAGACGAATTACGGGCTAAGAGGCCTAGTATGGGTGCAGTGGTAAAAGAGATAGAGAATATAATACAGATTTCTGCTACTAATTGGAACGCCGGTGCCAATTCAGCAGCTACTTCAGTAAGTTATGAGGATTTTTCAAATCCAATTGGGGCTTCATCGGTTGCCAAGGGAGATATTGGTATGAGTAGTATGGGGGAAAGCTTTTCCAATCGGGTTGACATGGATATTGATGATGATAATGAATCTGTTGATTTGACTAGCATACATAATCTGGGAGAAGCTTTTTTGAAGGATTTCTGTAAGGAGGCATCAGTTTCATTCTTCAACCAGTATGGCCTCATTAGTCACCAAATAAACTCGTACAATGACTTCATTAAAAATGGTATACAAAGAGTCTTTGATAGTTTTGATGAGATTATAGTTGAGCCTGGGTATGACCCATCGAAGAAGGGGAATGCAGAATGGCGATATGCTTCCATCAGGTTTGGGAAGGTTACCCTCATTCGACCAAGTTTTTGGGGCGGTGCTGTGAATGATAAGGAGTATAATATGCTACCTAGGCATGCTCGGCTTCAAAATATGACATACTCAGCCAAGATCAAAGTGAACGTTACTGTTGAGGTATATACTCAAAATCTTGTCAGAAGTGAAAAGTTTAAACTGGTAAAGAACAATGTCTTGACAAGCAGATTGTGA

Protein Analysis

1044

Amino Acids

114.34

Weight (kDa)

5.97

Isoelectric Point (pI)

29.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 78 - 166 1.3e-07 Leucine-rich repeat region
Pkinase PF00069 622 - 711 9.3e-13 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 624 - 708 3.2e-16 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 717 - 829 8.1e-07 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 718 - 826 1.2e-06 Protein kinase domain
RNA_pol_Rpb2_1 PF04563 925 - 1031 2.8e-12 RNA polymerase beta subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49760 AT5G49770
fragaria_vesca FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23881 FvH4_3g23881 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_5g27161 FvH4_5g27170
malus_domestica MD11G1206300.v1.1
prunus_persica Prupe.4G230700_v2.0.a1 Prupe.4G230700_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1
pyrus_communis pycom11g17930
rosa_chinensis RchiOBHm_Chr5g0042211 RchiOBHm_Chr5g0042261 RchiOBHm_Chr5g0042271 RchiOBHm_Chr5g0042311 RchiOBHm_Chr5g0042381 RchiOBHm_Chr7g0218261
rosa_laevigata RLG00000002384 RLG00000002385 RLG00000034112 RLG00000034114 RLG00000034116 RLG00000034118
rosa_multiflora Rmu_sc0001715.1_g000008 Rmu_sc0001715.1_g000012 Rmu_sc0001715.1_g000026 Rmu_sc0004956.1_g000008
rosa_roxburghii Rroxscaffold_1G00038360 Rroxscaffold_1G00038390 Rroxscaffold_1G00038400 Rroxscaffold_1G00038450 Rroxscaffold_3G00241180
rosa_rugosa Rorug05G0198800 Rorug05G0199000 Rorug05G0199100.1 Rorug05G0199200 Rorug05G0199300 Rorug07G0175900
rosa_samantha Rh5AG283400 Rh5AG283900 Rh5AG284100 Rh5AG284300 Rh5AG284900 Rh5AG285300 Rh5AG285700 Rh5BG289300 Rh5BG289900 Rh5BG290200 Rh5BG290400 Rh5CG320700 Rh5CG321200 Rh5CG321500 Rh5CG322100 Rh5CG322400 Rh5DG298600 Rh5DG299100 Rh5DG299400 Rh5DG300200 Rh7AG317500 Rh7BG308900 Rh7CG334400 Rh7DG316500
rosa_wichuraiana Rw0G001480 Rw0G001500 Rw0G001750 Rw0G001780 Rw0G015430 Rw5G026520 Rw7G026960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2534
AccB7I CCANNNNNTGG 1 cut(s) 1760
AccI GTMKAC 2 cut(s) 2442, 2824
AciI CCGC 5 cut(s) 461, 731, 1030, 1102, 2961
AclI AACGTT 1 cut(s) 3048
AcoI YGGCCR 3 cut(s) 480, 648, 1897
AcsI RAATTY 6 cut(s) 273, 280, 748, 859, 1009, 1360
AcuI CTGAAG 6 cut(s) 503, 956, 1263, 1530, 1862, 2538
AcvI CACGTG 1 cut(s) 1760
AcyI GRCGYC 1 cut(s) 1588
AfiI CCNNNNNNNGG 7 cut(s) 161, 470, 1325, 1435, 1466, 1760, 2471
AflII CTTAAG 1 cut(s) 572
AhlI ACTAGT 1 cut(s) 343
AjnI CCWGG 7 cut(s) 357, 738, 917, 1284, 1463, 1899, 2866
AjuI GAANNNNNNNTTGG 6 cut(s) 448, 480, 1863, 1895, 2575, 2607
Alw21I GWGCWC 2 cut(s) 13, 185
Alw26I GTCTC 4 cut(s) 541, 574, 1010, 1740
Alw44I GTGCAC 1 cut(s) 181
Ama87I CYCGRG 2 cut(s) 12, 610
ApaI GGGCCC 1 cut(s) 617
ApaLI GTGCAC 1 cut(s) 181
ApeKI GCWGC 4 cut(s) 65, 1099, 1632, 2546
ApoI RAATTY 6 cut(s) 273, 280, 748, 859, 1009, 1360
ArsI GACNNNNNNTTYG 4 cut(s) 1223, 1255, 2937, 2969
AseI ATTAAT 2 cut(s) 950, 1700
Asp700I GAANNNNTTC 1 cut(s) 2732
AspA2I CCTAGG 1 cut(s) 2996
AspLEI GCGC 2 cut(s) 1527, 1978
AsuHPI GGTGA 6 cut(s) 154, 1233, 1526, 2075, 2111, 2777
AvaI CYCGRG 2 cut(s) 12, 610
AvaII GGWCC 4 cut(s) 380, 1604, 1799, 2056
AvrII CCTAGG 1 cut(s) 2996
BaeGI GKGCMC 3 cut(s) 185, 617, 994
BaeI ACNNNNGTAYC 2 cut(s) 1209, 1242
BalI TGGCCA 3 cut(s) 482, 650, 1899
BamHI GGATCC 3 cut(s) 524, 1139, 2188
BanI GGYRCC 1 cut(s) 2534
BanII GRGCYC 1 cut(s) 617
BarI GAAGNNNNNNTAC 6 cut(s) 404, 436, 682, 714, 2792, 2824
BauI CACGAG 1 cut(s) 987
BbrPI CACGTG 1 cut(s) 1760
BbsI GAAGAC 2 cut(s) 1330, 1495
Bbv12I GWGCWC 2 cut(s) 13, 185
BbvI GCAGC 4 cut(s) 77, 1111, 1644, 2558
BccI CCATC 8 cut(s) 536, 640, 737, 949, 1516, 1762, 2887, 2924
BceAI ACGGC 1 cut(s) 178
BciT130I CCWGG 7 cut(s) 359, 740, 919, 1286, 1465, 1901, 2868
BclI TGATCA 2 cut(s) 1516, 2392
BcoDI GTCTC 4 cut(s) 541, 574, 1010, 1740
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 7 cut(s) 135, 344, 1334, 1490, 2466, 2697, 2997
BfmI CTRYAG 3 cut(s) 396, 1097, 1321
BfrI CTTAAG 1 cut(s) 572
BisI GCNGC 4 cut(s) 66, 1100, 1633, 2547
BlnI CCTAGG 1 cut(s) 2996
BlsI GCNGC 4 cut(s) 67, 1101, 1634, 2548
BmcAI AGTACT 1 cut(s) 2364
Bme1390I CCNGG 7 cut(s) 359, 740, 919, 1286, 1465, 1901, 2868
Bme18I GGWCC 4 cut(s) 380, 1604, 1799, 2056
BmeT110I CYCGRG 2 cut(s) 12, 610
BmiI GGNNCC 7 cut(s) 526, 615, 912, 1141, 1606, 2190, 2536
BmrFI CCNGG 7 cut(s) 359, 740, 919, 1286, 1465, 1901, 2868
BmrI ACTGGG 1 cut(s) 527
BmsI GCATC 5 cut(s) 204, 702, 1322, 1679, 2756
BmuI ACTGGG 1 cut(s) 527
BpiI GAAGAC 2 cut(s) 1330, 1495
BpmI CTGGAG 2 cut(s) 1054, 1659
BpuEI CTTGAG 3 cut(s) 1412, 1505, 2145
BsaAI YACGTR 1 cut(s) 1760
BsaHI GRCGYC 1 cut(s) 1588
BsaJI CCNNGG 6 cut(s) 1464, 1992, 2059, 2604, 2867, 2996
Bsc4I CCNNNNNNNGG 7 cut(s) 161, 470, 1325, 1435, 1466, 1760, 2471
Bse118I RCCGGY 1 cut(s) 2531
Bse1I ACTGG 8 cut(s) 49, 533, 835, 856, 1078, 1164, 2767, 3105
Bse3DI GCAATG 3 cut(s) 722, 2331, 2436
BseBI CCWGG 7 cut(s) 359, 740, 919, 1286, 1465, 1901, 2868
BseDI CCNNGG 6 cut(s) 1464, 1992, 2059, 2604, 2867, 2996
BseGI GGATG 6 cut(s) 118, 528, 693, 748, 1063, 1670
BseLI CCNNNNNNNGG 7 cut(s) 161, 470, 1325, 1435, 1466, 1760, 2471
BseMI GCAATG 3 cut(s) 722, 2331, 2436
BseMII CTCAG 7 cut(s) 95, 255, 1057, 1971, 2184, 2211, 3042
BseNI ACTGG 8 cut(s) 49, 533, 835, 856, 1078, 1164, 2767, 3105
BseRI GAGGAG 2 cut(s) 1753, 1758
BseSI GKGCMC 3 cut(s) 185, 617, 994
BseXI GCAGC 4 cut(s) 77, 1111, 1644, 2558
BseYI CCCAGC 1 cut(s) 165
BsgI GTGCAG 2 cut(s) 791, 2496
BshNI GGYRCC 1 cut(s) 2534
BsiHKAI GWGCWC 2 cut(s) 13, 185
BsiHKCI CYCGRG 2 cut(s) 12, 610
BsiSI CCGG 2 cut(s) 1629, 2532
BslFI GGGAC 4 cut(s) 321, 559, 1668, 2185
BslI CCNNNNNNNGG 7 cut(s) 161, 470, 1325, 1435, 1466, 1760, 2471
BsmAI GTCTC 4 cut(s) 541, 574, 1010, 1740
BsmFI GGGAC 4 cut(s) 321, 559, 1668, 2185
BsmI GAATGC 1 cut(s) 2899
BsoBI CYCGRG 2 cut(s) 12, 610
Bsp120I GGGCCC 1 cut(s) 613
Bsp1286I GDGCHC 4 cut(s) 13, 185, 617, 994
Bsp1407I TGTACA 1 cut(s) 1259
BspACI CCGC 5 cut(s) 461, 731, 1030, 1102, 2961
BspCNI CTCAG 7 cut(s) 94, 256, 1056, 1972, 2185, 2212, 3041
BspHI TCATGA 2 cut(s) 1773, 2356
BspLI GGNNCC 7 cut(s) 526, 615, 912, 1141, 1606, 2190, 2536
BspMAI CTGCAG 1 cut(s) 1101
BspQI GCTCTTC 1 cut(s) 1830
BspT107I GGYRCC 1 cut(s) 2534
BspTI CTTAAG 1 cut(s) 572
BsrDI GCAATG 3 cut(s) 722, 2331, 2436
BsrFI RCCGGY 1 cut(s) 2531
BsrGI TGTACA 1 cut(s) 1259
BsrI ACTGG 8 cut(s) 49, 533, 835, 856, 1078, 1164, 2767, 3105
BssAI RCCGGY 1 cut(s) 2531
BssECI CCNNGG 6 cut(s) 1464, 1992, 2059, 2604, 2867, 2996
BssNAI GTATAC 1 cut(s) 2825
BssNI GRCGYC 1 cut(s) 1588
BssSI CACGAG 1 cut(s) 987
BssT1I CCWWGG 3 cut(s) 2059, 2604, 2996
Bst1107I GTATAC 1 cut(s) 2825
Bst2BI CACGAG 1 cut(s) 987
Bst2UI CCWGG 7 cut(s) 359, 740, 919, 1286, 1465, 1901, 2868
Bst6I CTCTTC 4 cut(s) 1001, 1830, 1923, 2219
BstACI GRCGYC 1 cut(s) 1588
BstAFI CTTAAG 1 cut(s) 572
BstAUI TGTACA 1 cut(s) 1259
BstBAI YACGTR 1 cut(s) 1760
BstC8I GCNNGC 3 cut(s) 236, 729, 3003
BstEII GGTNACC 1 cut(s) 2932
BstF5I GGATG 6 cut(s) 118, 528, 693, 748, 1063, 1670
BstHHI GCGC 2 cut(s) 1527, 1978
BstMAI GTCTC 4 cut(s) 541, 574, 1010, 1740
BstMWI GCNNNNNNNGC 8 cut(s) 23, 71, 460, 1975, 2287, 2543, 2998, 3007
BstNI CCWGG 7 cut(s) 359, 740, 919, 1286, 1465, 1901, 2868
BstNSI RCATGY 2 cut(s) 100, 3005
BstPI GGTNACC 1 cut(s) 2932
BstSCI CCNGG 7 cut(s) 357, 738, 917, 1284, 1463, 1899, 2866
BstSFI CTRYAG 3 cut(s) 396, 1097, 1321
BstSLI GKGCMC 3 cut(s) 185, 617, 994
BstV1I GCAGC 4 cut(s) 77, 1111, 1644, 2558
BstV2I GAAGAC 2 cut(s) 1330, 1495
BstX2I RGATCY 5 cut(s) 401, 524, 1139, 1883, 2188
BstXI CCANNNNNNTGG 1 cut(s) 2955
BstYI RGATCY 5 cut(s) 401, 524, 1139, 1883, 2188
BstZ17I GTATAC 1 cut(s) 2825
BtsCI GGATG 6 cut(s) 118, 528, 693, 748, 1063, 1670
BtsI GCAGTG 2 cut(s) 198, 2484
BtsIMutI CAGTG 7 cut(s) 198, 454, 1026, 1051, 1801, 2143, 2484
Cac8I GCNNGC 3 cut(s) 236, 729, 3003
CciI TCATGA 2 cut(s) 1773, 2356
CfoI GCGC 2 cut(s) 1527, 1978
Cfr10I RCCGGY 1 cut(s) 2531
CseI GACGC 2 cut(s) 401, 1577
CsiI ACCWGGT 2 cut(s) 357, 917
CspCI CAANNNNNGTGG 2 cut(s) 667, 702
DraI TTTAAA 1 cut(s) 3097
EaeI YGGCCR 3 cut(s) 480, 648, 1897
Eam1104I CTCTTC 4 cut(s) 1001, 1830, 1923, 2219
EarI CTCTTC 4 cut(s) 1001, 1830, 1923, 2219
EciI GGCGGA 1 cut(s) 450
Eco130I CCWWGG 3 cut(s) 2059, 2604, 2996
Eco147I AGGCCT 1 cut(s) 2464
Eco24I GRGCYC 1 cut(s) 617
Eco32I GATATC 2 cut(s) 250, 1388
Eco47I GGWCC 4 cut(s) 380, 1604, 1799, 2056
Eco57I CTGAAG 6 cut(s) 503, 956, 1263, 1530, 1862, 2538
Eco72I CACGTG 1 cut(s) 1760
Eco88I CYCGRG 2 cut(s) 12, 610
Eco91I GGTNACC 1 cut(s) 2932
EcoO109I RGGNCCY 1 cut(s) 298
EcoO65I GGTNACC 1 cut(s) 2932
EcoRI GAATTC 1 cut(s) 859
EcoRII CCWGG 7 cut(s) 357, 738, 917, 1284, 1463, 1899, 2866
EcoRV GATATC 2 cut(s) 250, 1388
EcoT14I CCWWGG 3 cut(s) 2059, 2604, 2996
EcoT38I GRGCYC 1 cut(s) 617
ErhI CCWWGG 3 cut(s) 2059, 2604, 2996
FaqI GGGAC 4 cut(s) 321, 559, 1668, 2185
FbaI TGATCA 2 cut(s) 1516, 2392
FblI GTMKAC 2 cut(s) 2442, 2824
Fnu4HI GCNGC 4 cut(s) 66, 1100, 1633, 2547
FokI GGATG 6 cut(s) 125, 515, 680, 755, 1070, 1657
FriOI GRGCYC 1 cut(s) 617
Fsp4HI GCNGC 4 cut(s) 66, 1100, 1633, 2547
FspBI CTAG 7 cut(s) 135, 344, 1334, 1490, 2466, 2697, 2997
GlaI GCGC 2 cut(s) 1526, 1977
GluI GCNGC 4 cut(s) 66, 1100, 1633, 2547
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 2 cut(s) 1054, 1659
HapII CCGG 2 cut(s) 1629, 2532
HgaI GACGC 2 cut(s) 401, 1577
HhaI GCGC 2 cut(s) 1527, 1978
Hin1I GRCGYC 1 cut(s) 1588
Hin6I GCGC 2 cut(s) 1525, 1976
HinP1I GCGC 2 cut(s) 1525, 1976
HincII GTYRAC 2 cut(s) 2217, 2656
HindII GTYRAC 2 cut(s) 2217, 2656
HindIII AAGCTT 4 cut(s) 515, 935, 2638, 2718
HpaII CCGG 2 cut(s) 1629, 2532
HphI GGTGA 6 cut(s) 154, 1233, 1526, 2075, 2111, 2777
HpyCH4IV ACGT 2 cut(s) 1759, 3048
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 71, 460, 1975, 2287, 2543, 2998, 3007
HpySE526I ACGT 2 cut(s) 1759, 3048
Hsp92I GRCGYC 1 cut(s) 1588
HspAI GCGC 2 cut(s) 1525, 1976
Ksp22I TGATCA 2 cut(s) 1516, 2392
LguI GCTCTTC 1 cut(s) 1830
LmnI GCTCC 3 cut(s) 1541, 1814, 2260
Lsp1109I GCAGC 4 cut(s) 77, 1111, 1644, 2558
LweI GCATC 5 cut(s) 204, 702, 1322, 1679, 2756
MabI ACCWGGT 2 cut(s) 357, 917
MaeI CTAG 7 cut(s) 135, 344, 1334, 1490, 2466, 2697, 2997
MaeII ACGT 2 cut(s) 1759, 3048
MaeIII GTNAC 8 cut(s) 92, 158, 567, 1239, 2152, 2783, 2932, 3049
MfeI CAATTG 3 cut(s) 324, 1551, 2583
MflI RGATCY 5 cut(s) 401, 524, 1139, 1883, 2188
MhlI GDGCHC 4 cut(s) 13, 185, 617, 994
MlsI TGGCCA 3 cut(s) 482, 650, 1899
MluNI TGGCCA 3 cut(s) 482, 650, 1899
MlyI GAGTC 6 cut(s) 1195, 1391, 1570, 1742, 2200, 2841
MmeI TCCRAC 5 cut(s) 688, 885, 1167, 1241, 2238
Mox20I TGGCCA 3 cut(s) 482, 650, 1899
MroXI GAANNNNTTC 1 cut(s) 2732
MscI TGGCCA 3 cut(s) 482, 650, 1899
Msp20I TGGCCA 3 cut(s) 482, 650, 1899
MspA1I CMGCKG 2 cut(s) 1102, 2398
MspCI CTTAAG 1 cut(s) 572
MspI CCGG 2 cut(s) 1629, 2532
MspR9I CCNGG 7 cut(s) 359, 740, 919, 1286, 1465, 1901, 2868
MssI GTTTAAAC 1 cut(s) 3097
MunI CAATTG 3 cut(s) 324, 1551, 2583
Mva1269I GAATGC 1 cut(s) 2899
MvaI CCWGG 7 cut(s) 359, 740, 919, 1286, 1465, 1901, 2868
MwoI GCNNNNNNNGC 8 cut(s) 23, 71, 460, 1975, 2287, 2543, 2998, 3007
NlaIV GGNNCC 7 cut(s) 526, 615, 912, 1141, 1606, 2190, 2536
NmeAIII GCCGAG 2 cut(s) 2017, 2986
NmuCI GTSAC 3 cut(s) 158, 1239, 2783
NspI RCATGY 2 cut(s) 100, 3005
PaeI GCATGC 1 cut(s) 3005
PaeR7I CTCGAG 1 cut(s) 12
PagI TCATGA 2 cut(s) 1773, 2356
PceI AGGCCT 1 cut(s) 2464
PciSI GCTCTTC 1 cut(s) 1830
PctI GAATGC 1 cut(s) 2899
PdmI GAANNNNTTC 1 cut(s) 2732
PfeI GAWTC 6 cut(s) 103, 1439, 1961, 2026, 2133, 2681
PflMI CCANNNNNTGG 1 cut(s) 1760
PkrI GCNGC 4 cut(s) 67, 1101, 1634, 2548
PleI GAGTC 6 cut(s) 1194, 1391, 1570, 1742, 2200, 2840
PmaCI CACGTG 1 cut(s) 1760
PmeI GTTTAAAC 1 cut(s) 3097
PmlI CACGTG 1 cut(s) 1760
PpsI GAGTC 6 cut(s) 1194, 1391, 1570, 1742, 2200, 2840
Ppu21I YACGTR 1 cut(s) 1760
PshBI ATTAAT 2 cut(s) 950, 1700
Psp1406I AACGTT 1 cut(s) 3048
Psp6I CCWGG 7 cut(s) 357, 738, 917, 1284, 1463, 1899, 2866
PspCI CACGTG 1 cut(s) 1760
PspEI GGTNACC 1 cut(s) 2932
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 7 cut(s) 357, 738, 917, 1284, 1463, 1899, 2866
PspN4I GGNNCC 7 cut(s) 526, 615, 912, 1141, 1606, 2190, 2536
PspOMI GGGCCC 1 cut(s) 613
PspXI VCTCGAGB 1 cut(s) 12
PstI CTGCAG 1 cut(s) 1101
PsuI RGATCY 5 cut(s) 401, 524, 1139, 1883, 2188
PvuII CAGCTG 1 cut(s) 2398
SapI GCTCTTC 1 cut(s) 1830
SatI GCNGC 4 cut(s) 66, 1100, 1633, 2547
ScaI AGTACT 1 cut(s) 2364
SchI GAGTC 6 cut(s) 1195, 1391, 1570, 1742, 2200, 2841
ScrFI CCNGG 7 cut(s) 359, 740, 919, 1286, 1465, 1901, 2868
SduI GDGCHC 4 cut(s) 13, 185, 617, 994
SexAI ACCWGGT 2 cut(s) 357, 917
SfaNI GCATC 5 cut(s) 204, 702, 1322, 1679, 2756
SfcI CTRYAG 3 cut(s) 396, 1097, 1321
Sfr274I CTCGAG 1 cut(s) 12
SgrAI CRCCGGYG 1 cut(s) 2531
SinI GGWCC 4 cut(s) 380, 1604, 1799, 2056
SlaI CTCGAG 1 cut(s) 12
SmlI CTYRAG 5 cut(s) 12, 572, 1391, 1484, 2160
SmoI CTYRAG 5 cut(s) 12, 572, 1391, 1484, 2160
SpeI ACTAGT 1 cut(s) 343
SphI GCATGC 1 cut(s) 3005
SseBI AGGCCT 1 cut(s) 2464
SsiI CCGC 5 cut(s) 461, 731, 1030, 1102, 2961
SspMI CTAG 7 cut(s) 135, 344, 1334, 1490, 2466, 2697, 2997
StuI AGGCCT 1 cut(s) 2464
StyD4I CCNGG 7 cut(s) 357, 738, 917, 1284, 1463, 1899, 2866
StyI CCWWGG 3 cut(s) 2059, 2604, 2996
TaiI ACGT 2 cut(s) 1762, 3051
TaqI TCGA 8 cut(s) 13, 252, 316, 628, 773, 1581, 2882, 2944
TatI WGTACW 5 cut(s) 346, 1259, 2014, 2298, 2362
TfiI GAWTC 6 cut(s) 103, 1439, 1961, 2026, 2133, 2681
TscAI CASTG 7 cut(s) 205, 454, 1026, 1051, 1801, 2143, 2484
TseFI GTSAC 3 cut(s) 158, 1239, 2783
TseI GCWGC 4 cut(s) 65, 1099, 1632, 2546
Tsp45I GTSAC 3 cut(s) 158, 1239, 2783
TspRI CASTG 7 cut(s) 205, 454, 1026, 1051, 1801, 2143, 2484
Van91I CCANNNNNTGG 1 cut(s) 1760
Vha464I CTTAAG 1 cut(s) 572
VneI GTGCAC 1 cut(s) 181
VpaK11BI GGWCC 4 cut(s) 380, 1604, 1799, 2056
VspI ATTAAT 2 cut(s) 950, 1700
XapI RAATTY 6 cut(s) 273, 280, 748, 859, 1009, 1360
XceI RCATGY 2 cut(s) 100, 3005
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XhoI CTCGAG 1 cut(s) 12
XmaJI CCTAGG 1 cut(s) 2996
XmiI GTMKAC 2 cut(s) 2442, 2824
XmnI GAANNNNTTC 1 cut(s) 2732
XspI CTAG 7 cut(s) 135, 344, 1334, 1490, 2466, 2697, 2997
ZrmI AGTACT 1 cut(s) 2364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.