Rh5BG289300

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000487
Physical Location & Seq
Reverse (-)
36791704 .. 36793502
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG289300.1

Sequence Viewer

Length: 1224 bp
ATGATGGAAAACATAGGACTTCAGGGACAAGTTCCTCGAGCCCTTTTCAGCCTTGAATATTTGGAGTCAGTGGTTCTAAGAAACAACAATCTCAGTGATTCATTGGATATTGGAACCAATTATAGCAAACAATTGGAGCTAGTTGATCTGCAGAAGAACAACATTGTCGTTCTTGCACAGAACTATGATGGATCCAACTACACGCTGATTCTAGTTGAAAATCCAATTTGTGAGGGAAGCAAGTACAACATGGCAAAGAAGTACTGTAATATTTCTCCACCAGATTCCTCAATTTCAACTCCAGGAAGTAACTGTCTTACTCCTGTCTGTAAATCCGACCAGGTTGCTTCCCCCAACTGTAATTGTGCATTTCCCTACACACAGACTCTAGTCTTCTTATTTGTTTCTTACTCAAACTTGGAAGACGTAAACTATTACATTCTTCTCGCGGAAAATCTCACAAGATCATTACAGTCCCTCAACCTTCCTGTGGATTCAGTTTCTCTGAGTTATCCTAAATTGGACTCATCTTCTCAACTTGAACTGACAATACAAATTTTCCCATCTGGTGGTCAAGTTAGCTTCACCCAAGCAGAAGAGTCAGCTATATCAACTGTTCTTAGCAACCAGACTCTCGGTGCAAGTCCAAGATATTTTGGACCCTTCTCTTTTCTTGTTCTTTACACCAACTCTGGAGGATCAAACAAGGCCGTGATAATAGGTACAGCAATTGGTGGCTGTGTGCTCCTTTCATTATTAGTCCTTGTAGGGCTTTATGCTCTCCAGCAGAAGAGGAGAGCCGACGAGTCATTGAGTGGATCAATACTTTTGCAGACAGGGATTTCAAACAGCAGTGCTAGTGGTCCTCAACTAAAAGGAGCAAGATTGTTCTCATTTGAAGAGCTCAAGAAATGCACCGATGGTTTTTCAAAAGCAAAAGATATTGGATCTGGTGGTTATGGCCAGGTATATCAGGGAATTCTTCCTACAGGCCAGATGGTTGCCATAAAAAGAGCTAAAAGGGAATCTATGCAGGGAGGAGTTGAGTTCAAAGCCGAGGTTGAGCTCCTATCACGAGTACATCATAAGAATCTTGTTGGCCTTGTTGGTTTTTGTTTTGAACAAGGAGAACAAATATTGGTATATCAGTATGTTCCAAATGGTGATTTAAGGGACAGCCTATCAGGTATTCCATGCACTTGCACTTTACAAAGCTTGTTATAG

Protein Analysis

407

Amino Acids

44.1

Weight (kDa)

5.28

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 310 - 395 8.4e-13 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 312 - 395 2.4e-15 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49760 AT5G49770
fragaria_vesca FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23881 FvH4_3g23881 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_5g27161 FvH4_5g27170
malus_domestica MD11G1206300.v1.1
prunus_persica Prupe.4G230700_v2.0.a1 Prupe.4G230700_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1
pyrus_communis pycom11g17930
rosa_chinensis RchiOBHm_Chr5g0042211 RchiOBHm_Chr5g0042261 RchiOBHm_Chr5g0042271 RchiOBHm_Chr5g0042311 RchiOBHm_Chr5g0042381 RchiOBHm_Chr7g0218261
rosa_laevigata RLG00000002384 RLG00000002385 RLG00000034112 RLG00000034114 RLG00000034116 RLG00000034118
rosa_multiflora Rmu_sc0001715.1_g000008 Rmu_sc0001715.1_g000012 Rmu_sc0001715.1_g000026 Rmu_sc0004956.1_g000008
rosa_roxburghii Rroxscaffold_1G00038360 Rroxscaffold_1G00038390 Rroxscaffold_1G00038400 Rroxscaffold_1G00038450 Rroxscaffold_3G00241180
rosa_rugosa Rorug05G0198800 Rorug05G0199000 Rorug05G0199100.1 Rorug05G0199200 Rorug05G0199300 Rorug07G0175900
rosa_samantha Rh5AG283400 Rh5AG283900 Rh5AG284100 Rh5AG284300 Rh5AG284900 Rh5AG285300 Rh5AG285700 Rh5BG289300 Rh5BG289900 Rh5BG290200 Rh5BG290400 Rh5CG320700 Rh5CG321200 Rh5CG321500 Rh5CG322100 Rh5CG322400 Rh5DG298600 Rh5DG299100 Rh5DG299400 Rh5DG300200 Rh7AG317500 Rh7BG308900 Rh7CG334400 Rh7DG316500
rosa_wichuraiana Rw0G001480 Rw0G001500 Rw0G001750 Rw0G001780 Rw0G015430 Rw5G026520 Rw7G026960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 569
AccII CGCG 1 cut(s) 449
AciI CCGC 1 cut(s) 449
AclWI GGATC 5 cut(s) 186, 199, 706, 826, 955
AcoI YGGCCR 1 cut(s) 961
AcsI RAATTY 2 cut(s) 555, 978
AcuI CTGAAG 1 cut(s) 5
AfaI GTAC 4 cut(s) 245, 263, 724, 1080
AfiI CCNNNNNNNGG 2 cut(s) 490, 569
AgsI TTSAA 9 cut(s) 56, 218, 297, 542, 846, 899, 930, 1051, 1121
AjnI CCWGG 3 cut(s) 301, 339, 963
AluBI AGCT 7 cut(s) 139, 582, 605, 904, 1016, 1066, 1215
AluI AGCT 7 cut(s) 139, 582, 605, 904, 1016, 1066, 1215
Alw21I GWGCWC 3 cut(s) 747, 906, 1068
AlwI GGATC 5 cut(s) 186, 199, 706, 826, 955
Ama87I CYCGRG 1 cut(s) 36
AoxI GGCC 4 cut(s) 708, 961, 991, 1099
ApoI RAATTY 2 cut(s) 555, 978
AspS9I GGNCC 2 cut(s) 659, 863
AsuHPI GGTGA 2 cut(s) 577, 1175
AvaI CYCGRG 1 cut(s) 36
AvaII GGWCC 2 cut(s) 659, 863
BalI TGGCCA 1 cut(s) 963
BamHI GGATCC 1 cut(s) 191
BanII GRGCYC 3 cut(s) 43, 906, 1068
BauI CACGAG 1 cut(s) 1074
BbsI GAAGAC 2 cut(s) 385, 429
Bbv12I GWGCWC 3 cut(s) 747, 906, 1068
BccI CCATC 4 cut(s) 182, 571, 914, 991
BceAI ACGGC 1 cut(s) 695
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 3 cut(s) 303, 341, 965
BfaI CTAG 4 cut(s) 140, 212, 389, 858
BfmI CTRYAG 2 cut(s) 149, 987
BmcAI AGTACT 1 cut(s) 263
Bme1390I CCNGG 3 cut(s) 303, 341, 965
Bme18I GGWCC 2 cut(s) 659, 863
BmeT110I CYCGRG 1 cut(s) 36
BmgT120I GGNCC 2 cut(s) 659, 863
BmiI GGNNCC 3 cut(s) 115, 193, 661
BmrFI CCNGG 3 cut(s) 303, 341, 965
BoxI GACNNNNGTC 1 cut(s) 389
BpiI GAAGAC 2 cut(s) 385, 429
BpmI CTGGAG 3 cut(s) 285, 714, 767
BpuEI CTTGAG 1 cut(s) 890
BsaJI CCNNGG 1 cut(s) 1056
Bsc4I CCNNNNNNNGG 2 cut(s) 490, 569
BseBI CCWGG 3 cut(s) 303, 341, 965
BseDI CCNNGG 1 cut(s) 1056
BseLI CCNNNNNNNGG 2 cut(s) 490, 569
BseMII CTCAG 2 cut(s) 106, 497
BseRI GAGGAG 2 cut(s) 808, 1053
Bsh1236I CGCG 1 cut(s) 449
BshFI GGCC 4 cut(s) 710, 963, 993, 1101
BsiHKAI GWGCWC 3 cut(s) 747, 906, 1068
BsiHKCI CYCGRG 1 cut(s) 36
BslFI GGGAC 3 cut(s) 39, 460, 1187
BslI CCNNNNNNNGG 2 cut(s) 490, 569
BsmFI GGGAC 3 cut(s) 39, 460, 1187
BsnI GGCC 4 cut(s) 710, 963, 993, 1101
BsoBI CYCGRG 1 cut(s) 36
Bsp1286I GDGCHC 4 cut(s) 43, 747, 906, 1068
Bsp143I GATC 6 cut(s) 145, 191, 464, 698, 818, 947
BspACI CCGC 1 cut(s) 449
BspANI GGCC 4 cut(s) 710, 963, 993, 1101
BspCNI CTCAG 2 cut(s) 105, 498
BspFNI CGCG 1 cut(s) 449
BspLI GGNNCC 3 cut(s) 115, 193, 661
BspMAI CTGCAG 1 cut(s) 153
BspPI GGATC 5 cut(s) 186, 199, 706, 826, 955
BspQI GCTCTTC 1 cut(s) 894
BssECI CCNNGG 1 cut(s) 1056
BssMI GATC 6 cut(s) 145, 191, 464, 698, 818, 947
BssSI CACGAG 1 cut(s) 1074
Bst2BI CACGAG 1 cut(s) 1074
Bst2UI CCWGG 3 cut(s) 303, 341, 965
Bst4CI ACNGT 5 cut(s) 266, 314, 359, 474, 616
Bst6I CTCTTC 3 cut(s) 591, 785, 894
BstDEI CTNAG 4 cut(s) 77, 92, 506, 620
BstFNI CGCG 1 cut(s) 449
BstKTI GATC 6 cut(s) 148, 194, 467, 701, 821, 950
BstMBI GATC 6 cut(s) 145, 191, 464, 698, 818, 947
BstNI CCWGG 3 cut(s) 303, 341, 965
BstPAI GACNNNNGTC 1 cut(s) 389
BstSCI CCNGG 3 cut(s) 301, 339, 963
BstSFI CTRYAG 2 cut(s) 149, 987
BstUI CGCG 1 cut(s) 449
BstV2I GAAGAC 2 cut(s) 385, 429
BstX2I RGATCY 2 cut(s) 191, 947
BstYI RGATCY 2 cut(s) 191, 947
BsuRI GGCC 4 cut(s) 710, 963, 993, 1101
BtsI GCAGTG 1 cut(s) 859
BtsIMutI CAGTG 3 cut(s) 75, 100, 859
Cfr13I GGNCC 2 cut(s) 659, 863
CsiI ACCWGGT 1 cut(s) 339
Csp6I GTAC 4 cut(s) 244, 262, 723, 1079
CviAII CATG 2 cut(s) 250, 1194
CviQI GTAC 4 cut(s) 244, 262, 723, 1079
DdeI CTNAG 4 cut(s) 77, 92, 506, 620
DpnI GATC 6 cut(s) 147, 193, 466, 700, 820, 949
DpnII GATC 6 cut(s) 145, 191, 464, 698, 818, 947
EaeI YGGCCR 1 cut(s) 961
Eam1104I CTCTTC 3 cut(s) 591, 785, 894
EarI CTCTTC 3 cut(s) 591, 785, 894
Ecl136II GAGCTC 2 cut(s) 904, 1066
Eco24I GRGCYC 3 cut(s) 43, 906, 1068
Eco47I GGWCC 2 cut(s) 659, 863
Eco53kI GAGCTC 2 cut(s) 904, 1066
Eco57I CTGAAG 1 cut(s) 5
Eco88I CYCGRG 1 cut(s) 36
EcoICRI GAGCTC 2 cut(s) 904, 1066
EcoRI GAATTC 1 cut(s) 978
EcoRII CCWGG 3 cut(s) 301, 339, 963
EcoT38I GRGCYC 3 cut(s) 43, 906, 1068
FaeI CATG 2 cut(s) 253, 1197
FaqI GGGAC 3 cut(s) 39, 460, 1187
FatI CATG 2 cut(s) 249, 1193
FriOI GRGCYC 3 cut(s) 43, 906, 1068
FspBI CTAG 4 cut(s) 140, 212, 389, 858
GsuI CTGGAG 3 cut(s) 285, 714, 767
HaeIII GGCC 4 cut(s) 710, 963, 993, 1101
Hin1II CATG 2 cut(s) 253, 1197
HindIII AAGCTT 1 cut(s) 1213
HphI GGTGA 2 cut(s) 577, 1175
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 2 cut(s) 337, 507
Hpy188III TCNNGA 3 cut(s) 693, 907, 1074
Hpy8I GTNNAC 1 cut(s) 430
Hpy99I CGWCG 1 cut(s) 806
HpyAV CCTTC 2 cut(s) 494, 673
HpyCH4III ACNGT 5 cut(s) 266, 314, 359, 474, 616
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 9 cut(s) 151, 176, 368, 641, 832, 915, 1033, 1197, 1203
HpyF3I CTNAG 4 cut(s) 77, 92, 506, 620
HpySE526I ACGT 1 cut(s) 426
Hsp92II CATG 2 cut(s) 253, 1197
Kzo9I GATC 6 cut(s) 145, 191, 464, 698, 818, 947
LguI GCTCTTC 1 cut(s) 894
LmnI GCTCC 4 cut(s) 136, 750, 878, 1071
MabI ACCWGGT 1 cut(s) 339
MaeI CTAG 4 cut(s) 140, 212, 389, 858
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 1 cut(s) 308
MalI GATC 6 cut(s) 147, 193, 466, 700, 820, 949
MboI GATC 6 cut(s) 145, 191, 464, 698, 818, 947
MboII GAAGA 9 cut(s) 166, 385, 434, 434, 522, 608, 802, 911, 974
MfeI CAATTG 2 cut(s) 131, 729
MflI RGATCY 2 cut(s) 191, 947
MhlI GDGCHC 4 cut(s) 43, 747, 906, 1068
MlsI TGGCCA 1 cut(s) 963
MluCI AATT 9 cut(s) 118, 131, 225, 291, 361, 518, 555, 729, 978
MluNI TGGCCA 1 cut(s) 963
MlyI GAGTC 6 cut(s) 74, 379, 518, 608, 625, 815
MmeI TCCRAC 2 cut(s) 219, 360
MnlI CCTC 9 cut(s) 45, 226, 298, 488, 689, 786, 876, 1031, 1051
Mox20I TGGCCA 1 cut(s) 963
MscI TGGCCA 1 cut(s) 963
MseI TTAA 1 cut(s) 1169
Msp20I TGGCCA 1 cut(s) 963
MspR9I CCNGG 3 cut(s) 303, 341, 965
MunI CAATTG 2 cut(s) 131, 729
MvaI CCWGG 3 cut(s) 303, 341, 965
MvnI CGCG 1 cut(s) 449
NdeII GATC 6 cut(s) 145, 191, 464, 698, 818, 947
NlaIII CATG 2 cut(s) 253, 1197
NlaIV GGNNCC 3 cut(s) 115, 193, 661
NmeAIII GCCGAG 1 cut(s) 1081
PaeR7I CTCGAG 1 cut(s) 36
PciSI GCTCTTC 1 cut(s) 894
PfeI GAWTC 6 cut(s) 98, 208, 284, 494, 1025, 1090
PflMI CCANNNNNTGG 1 cut(s) 569
PfoI TCCNGGA 1 cut(s) 301
PleI GAGTC 6 cut(s) 73, 379, 518, 607, 625, 814
PpsI GAGTC 6 cut(s) 73, 379, 518, 607, 625, 814
PshAI GACNNNNGTC 1 cut(s) 389
Psp124BI GAGCTC 2 cut(s) 906, 1068
Psp6I CCWGG 3 cut(s) 301, 339, 963
PspGI CCWGG 3 cut(s) 301, 339, 963
PspN4I GGNNCC 3 cut(s) 115, 193, 661
PspPI GGNCC 2 cut(s) 659, 863
PspXI VCTCGAGB 1 cut(s) 36
PsrI GAACNNNNNNTAC 2 cut(s) 534, 566
PstI CTGCAG 1 cut(s) 153
PsuI RGATCY 2 cut(s) 191, 947
RsaI GTAC 4 cut(s) 245, 263, 724, 1080
RsaNI GTAC 4 cut(s) 244, 262, 723, 1079
SacI GAGCTC 2 cut(s) 906, 1068
SapI GCTCTTC 1 cut(s) 894
SaqAI TTAA 1 cut(s) 1169
Sau3AI GATC 6 cut(s) 145, 191, 464, 698, 818, 947
Sau96I GGNCC 2 cut(s) 659, 863
ScaI AGTACT 1 cut(s) 263
SchI GAGTC 6 cut(s) 74, 379, 518, 608, 625, 815
ScrFI CCNGG 3 cut(s) 303, 341, 965
SduI GDGCHC 4 cut(s) 43, 747, 906, 1068
SexAI ACCWGGT 1 cut(s) 339
SfcI CTRYAG 2 cut(s) 149, 987
Sfr274I CTCGAG 1 cut(s) 36
SinI GGWCC 2 cut(s) 659, 863
SlaI CTCGAG 1 cut(s) 36
SmlI CTYRAG 2 cut(s) 36, 905
SmoI CTYRAG 2 cut(s) 36, 905
Sse9I AATT 9 cut(s) 118, 131, 225, 291, 361, 518, 555, 729, 978
SsiI CCGC 1 cut(s) 449
SspI AATATT 3 cut(s) 59, 271, 1137
SspMI CTAG 4 cut(s) 140, 212, 389, 858
SstI GAGCTC 2 cut(s) 906, 1068
StyD4I CCNGG 3 cut(s) 301, 339, 963
TaaI ACNGT 5 cut(s) 266, 314, 359, 474, 616
TaiI ACGT 1 cut(s) 429
TaqI TCGA 1 cut(s) 37
TasI AATT 9 cut(s) 118, 131, 225, 291, 361, 518, 555, 729, 978
TatI WGTACW 3 cut(s) 243, 261, 1078
TfiI GAWTC 6 cut(s) 98, 208, 284, 494, 1025, 1090
Tru1I TTAA 1 cut(s) 1169
Tru9I TTAA 1 cut(s) 1169
TscAI CASTG 3 cut(s) 75, 100, 859
TspDTI ATGAA 2 cut(s) 90, 741
TspRI CASTG 3 cut(s) 75, 100, 859
Van91I CCANNNNNTGG 1 cut(s) 569
VpaK11BI GGWCC 2 cut(s) 659, 863
XapI RAATTY 2 cut(s) 555, 978
XhoI CTCGAG 1 cut(s) 36
XspI CTAG 4 cut(s) 140, 212, 389, 858
ZrmI AGTACT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.