Rh5CG322400

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000487
Physical Location & Seq
Reverse (-)
35638352 .. 35639724
1373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG322400.1

Sequence Viewer

Length: 999 bp
ATGGCCAAGAATTATTGCATTGTTGCTTCATCAGATTCCTTATACTCAACACCACCAAATAACTGTGTGCCCATTGGCTGTAGTTCGGGAAAAGTTTCCAGCCCGAATTGTAAATGTGCACATCCTTTCACAGCAACTCTAGTCTTCTTATATGTCAGTTTCTCAGACTTTGGAAACTCAACTTATTACACAGAGCTAGAGATCTCTCTCATCCAATCTTTTCGGACTTACAACATTCCTGTGGATTCAGTTTCTCTGGGTTCTCCTTTCTTCGACTTATTTTATTACTTTCACCTGATCCTAGAGGTTTTCCCATCTGATCAAGATCACTTCAACAAATCAGAAGCTTCAGCACTTGCTTCTGTGCTTAGTAACCAGACTCTAACACGTCCCCGGTATTTTGGACCCTTCTCTGTTGCATTAATTTATGCTCACTTCGGAGGATCAAACAAGGCCTTGATAATTGGTGCAGCAGTTGGCTGTTCTGTGCTTCTGTTATCAATAGCACTTGTAGGGGTTTATGCTCTCCAACAAAAGGGGAGAGCAAATGAGCAAGGAAGCAGAGCAGACGAGCAACGGAGCAGAGCACTCCCTTTGCAGCCTTGGATATCAAAAAGCAGTCTTCCTCAGTTAAAAGGAGCAAGATTGTTTTCTTTTAAAGAGCTTAAGGATTGCACCAATAGTTTTTCAGAAACAAATGATATTGGTTCCGGGGGTTATGGAAAGGTGTATCAGGGAGTACTTCCAACAGGCGAAATGGTTGCCATTAAACGAGCTAAAAGGGAATCTATCCAGGAAGGGATTGAGTTCAAAGCTGAGGTTGAGCTGCTTTCAAGGGTACATCATAAGAATCTTGTGAGCCTTGTTGGTTTTTGTTTGGAGCAAGATGAACAAATGTTGGTATACGAGTATGTTCCAAATGGTAACTTGATGGATAGTCTTTCAGGTAATCCATATACGGTATTGTTCTTCAATATAAATAAGAAATTAGGACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

332

Amino Acids

36.54

Weight (kDa)

6.99

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 230 - 315 1.2e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 232 - 317 2.9e-16 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49760 AT5G49770
fragaria_vesca FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23881 FvH4_3g23881 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_5g27161 FvH4_5g27170
malus_domestica MD11G1206300.v1.1
prunus_persica Prupe.4G230700_v2.0.a1 Prupe.4G230700_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1
pyrus_communis pycom11g17930
rosa_chinensis RchiOBHm_Chr5g0042211 RchiOBHm_Chr5g0042261 RchiOBHm_Chr5g0042271 RchiOBHm_Chr5g0042311 RchiOBHm_Chr5g0042381 RchiOBHm_Chr7g0218261
rosa_laevigata RLG00000002384 RLG00000002385 RLG00000034112 RLG00000034114 RLG00000034116 RLG00000034118
rosa_multiflora Rmu_sc0001715.1_g000008 Rmu_sc0001715.1_g000012 Rmu_sc0001715.1_g000026 Rmu_sc0004956.1_g000008
rosa_roxburghii Rroxscaffold_1G00038360 Rroxscaffold_1G00038390 Rroxscaffold_1G00038400 Rroxscaffold_1G00038450 Rroxscaffold_3G00241180
rosa_rugosa Rorug05G0198800 Rorug05G0199000 Rorug05G0199100.1 Rorug05G0199200 Rorug05G0199300 Rorug07G0175900
rosa_samantha Rh5AG283400 Rh5AG283900 Rh5AG284100 Rh5AG284300 Rh5AG284900 Rh5AG285300 Rh5AG285700 Rh5BG289300 Rh5BG289900 Rh5BG290200 Rh5BG290400 Rh5CG320700 Rh5CG321200 Rh5CG321500 Rh5CG322100 Rh5CG322400 Rh5DG298600 Rh5DG299100 Rh5DG299400 Rh5DG300200 Rh7AG317500 Rh7BG308900 Rh7CG334400 Rh7DG316500
rosa_wichuraiana Rw0G001480 Rw0G001500 Rw0G001750 Rw0G001780 Rw0G015430 Rw5G026520 Rw7G026960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 903
AclWI GGATC 2 cut(s) 292, 451
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 2 cut(s) 741, 840
AfiI CCNNNNNNNGG 1 cut(s) 535
AflII CTTAAG 1 cut(s) 665
AflIII ACRYGT 1 cut(s) 386
AgsI TTSAA 4 cut(s) 334, 811, 834, 973
AjiI CACGTC 1 cut(s) 389
AjnI CCWGG 1 cut(s) 792
AluBI AGCT 6 cut(s) 196, 347, 664, 776, 815, 826
AluI AGCT 6 cut(s) 196, 347, 664, 776, 815, 826
Alw21I GWGCWC 2 cut(s) 121, 589
Alw44I GTGCAC 1 cut(s) 117
AlwI GGATC 2 cut(s) 292, 451
AoxI GGCC 2 cut(s) 3, 453
ApaLI GTGCAC 1 cut(s) 117
ApeKI GCWGC 3 cut(s) 470, 598, 826
AseI ATTAAT 1 cut(s) 422
Asp700I GAANNNNTTC 1 cut(s) 94
AspS9I GGNCC 2 cut(s) 404, 992
AsuC2I CCSGG 2 cut(s) 394, 712
AsuHPI GGTGA 1 cut(s) 284
AvaII GGWCC 2 cut(s) 404, 992
BaeGI GKGCMC 2 cut(s) 72, 121
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 136, 614
Bbv12I GWGCWC 2 cut(s) 121, 589
BbvCI CCTCAGC 1 cut(s) 816
BbvI GCAGC 3 cut(s) 482, 610, 813
BccI CCATC 2 cut(s) 322, 925
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciT130I CCWGG 1 cut(s) 794
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 2 cut(s) 394, 712
BfaI CTAG 3 cut(s) 140, 197, 302
BfmI CTRYAG 1 cut(s) 79
BfrI CTTAAG 1 cut(s) 665
BglII AGATCT 1 cut(s) 201
BisI GCNGC 3 cut(s) 471, 599, 827
BlsI GCNGC 3 cut(s) 472, 600, 828
BmcAI AGTACT 1 cut(s) 741
Bme1390I CCNGG 3 cut(s) 394, 712, 794
Bme18I GGWCC 2 cut(s) 404, 992
BmgBI CACGTC 1 cut(s) 389
BmgT120I GGNCC 2 cut(s) 404, 992
BmiI GGNNCC 2 cut(s) 406, 709
BmrFI CCNGG 3 cut(s) 394, 712, 794
BpiI GAAGAC 2 cut(s) 136, 614
Bpu10I CCTNAGC 1 cut(s) 816
BpuMI CCSGG 2 cut(s) 394, 712
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 3 cut(s) 392, 602, 711
Bsc4I CCNNNNNNNGG 1 cut(s) 535
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 1 cut(s) 794
BseDI CCNNGG 3 cut(s) 392, 602, 711
BseGI GGATG 2 cut(s) 121, 210
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 1 cut(s) 535
BseMII CTCAG 3 cut(s) 177, 641, 807
BseSI GKGCMC 2 cut(s) 72, 121
BseXI GCAGC 3 cut(s) 482, 610, 813
BsgI GTGCAG 1 cut(s) 489
BshFI GGCC 2 cut(s) 5, 455
BsiHKAI GWGCWC 2 cut(s) 121, 589
BsiSI CCGG 2 cut(s) 394, 711
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 1 cut(s) 535
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 2 cut(s) 5, 455
Bsp1286I GDGCHC 3 cut(s) 72, 121, 589
Bsp143I GATC 5 cut(s) 201, 297, 319, 325, 443
BspANI GGCC 2 cut(s) 5, 455
BspCNI CTCAG 3 cut(s) 176, 640, 808
BspLI GGNNCC 2 cut(s) 406, 709
BspPI GGATC 2 cut(s) 292, 451
BspTI CTTAAG 1 cut(s) 665
BssECI CCNNGG 3 cut(s) 392, 602, 711
BssMI GATC 5 cut(s) 201, 297, 319, 325, 443
BssNAI GTATAC 1 cut(s) 904
BssT1I CCWWGG 1 cut(s) 602
Bst1107I GTATAC 1 cut(s) 904
Bst2UI CCWGG 1 cut(s) 794
Bst4CI ACNGT 2 cut(s) 65, 961
BstAFI CTTAAG 1 cut(s) 665
BstDEI CTNAG 4 cut(s) 163, 368, 627, 816
BstF5I GGATG 2 cut(s) 121, 210
BstKTI GATC 5 cut(s) 204, 300, 322, 328, 446
BstMBI GATC 5 cut(s) 201, 297, 319, 325, 443
BstNI CCWGG 1 cut(s) 794
BstSCI CCNGG 3 cut(s) 392, 710, 792
BstSFI CTRYAG 1 cut(s) 79
BstSLI GKGCMC 2 cut(s) 72, 121
BstV1I GCAGC 3 cut(s) 482, 610, 813
BstV2I GAAGAC 2 cut(s) 136, 614
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
BstZ17I GTATAC 1 cut(s) 904
BsuRI GGCC 2 cut(s) 5, 455
BtrI CACGTC 1 cut(s) 389
BtsCI GGATG 2 cut(s) 121, 210
Cfr13I GGNCC 2 cut(s) 404, 992
Csp6I GTAC 2 cut(s) 740, 839
CviQI GTAC 2 cut(s) 740, 839
DdeI CTNAG 4 cut(s) 163, 368, 627, 816
DpnI GATC 5 cut(s) 203, 299, 321, 327, 445
DpnII GATC 5 cut(s) 201, 297, 319, 325, 443
DraI TTTAAA 1 cut(s) 658
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 602
Eco147I AGGCCT 1 cut(s) 455
Eco32I GATATC 1 cut(s) 609
Eco47I GGWCC 2 cut(s) 404, 992
Eco57I CTGAAG 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 992
EcoRII CCWGG 1 cut(s) 792
EcoRV GATATC 1 cut(s) 609
EcoT14I CCWWGG 1 cut(s) 602
ErhI CCWWGG 1 cut(s) 602
FaqI GGGAC 1 cut(s) 375
FbaI TGATCA 1 cut(s) 319
FblI GTMKAC 1 cut(s) 903
Fnu4HI GCNGC 3 cut(s) 471, 599, 827
FokI GGATG 2 cut(s) 108, 197
Fsp4HI GCNGC 3 cut(s) 471, 599, 827
FspBI CTAG 3 cut(s) 140, 197, 302
GluI GCNGC 3 cut(s) 471, 599, 827
HaeIII GGCC 2 cut(s) 5, 455
HapII CCGG 2 cut(s) 394, 711
HindIII AAGCTT 1 cut(s) 345
HinfI GANTC 5 cut(s) 35, 245, 379, 785, 850
HpaII CCGG 2 cut(s) 394, 711
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 2 cut(s) 119, 904
Hpy188I TCNGA 7 cut(s) 34, 166, 225, 319, 343, 440, 691
Hpy188III TCNNGA 2 cut(s) 87, 323
Hpy8I GTNNAC 2 cut(s) 119, 904
HpyAV CCTTC 2 cut(s) 418, 791
HpyCH4III ACNGT 2 cut(s) 65, 961
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 6 cut(s) 18, 119, 419, 470, 598, 675
HpyF3I CTNAG 4 cut(s) 163, 368, 627, 816
HpySE526I ACGT 1 cut(s) 388
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 5 cut(s) 201, 297, 319, 325, 443
LmnI GCTCC 3 cut(s) 579, 638, 880
Lsp1109I GCAGC 3 cut(s) 482, 610, 813
MaeI CTAG 3 cut(s) 140, 197, 302
MaeII ACGT 1 cut(s) 388
MaeIII GTNAC 2 cut(s) 371, 923
MalI GATC 5 cut(s) 203, 299, 321, 327, 445
MboI GATC 5 cut(s) 201, 297, 319, 325, 443
MboII GAAGA 4 cut(s) 136, 262, 614, 961
MflI RGATCY 1 cut(s) 201
MhlI GDGCHC 3 cut(s) 72, 121, 589
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 10, 106, 423, 462, 986
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 373
MmeI TCCRAC 2 cut(s) 553, 770
MnlI CCTC 4 cut(s) 298, 434, 636, 811
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 94
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 422, 632, 657, 666, 768, 997
MslI CAYNNNNRTG 1 cut(s) 239
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 2 cut(s) 394, 711
MspR9I CCNGG 3 cut(s) 394, 712, 794
MvaI CCWGG 1 cut(s) 794
NciI CCSGG 2 cut(s) 394, 712
NdeII GATC 5 cut(s) 201, 297, 319, 325, 443
NlaIV GGNNCC 2 cut(s) 406, 709
PceI AGGCCT 1 cut(s) 455
PdmI GAANNNNTTC 1 cut(s) 94
PfeI GAWTC 4 cut(s) 35, 245, 785, 850
PfoI TCCNGGA 1 cut(s) 792
PkrI GCNGC 3 cut(s) 472, 600, 828
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
PpuMI RGGWCCY 1 cut(s) 992
PshBI ATTAAT 1 cut(s) 422
Psp5II RGGWCCY 1 cut(s) 992
Psp6I CCWGG 1 cut(s) 792
PspGI CCWGG 1 cut(s) 792
PspN4I GGNNCC 2 cut(s) 406, 709
PspPI GGNCC 2 cut(s) 404, 992
PspPPI RGGWCCY 1 cut(s) 992
PsuI RGATCY 1 cut(s) 201
RsaI GTAC 2 cut(s) 741, 840
RsaNI GTAC 2 cut(s) 740, 839
RseI CAYNNNNRTG 1 cut(s) 239
SaqAI TTAA 6 cut(s) 422, 632, 657, 666, 768, 997
SatI GCNGC 3 cut(s) 471, 599, 827
Sau3AI GATC 5 cut(s) 201, 297, 319, 325, 443
Sau96I GGNCC 2 cut(s) 404, 992
ScaI AGTACT 1 cut(s) 741
SchI GAGTC 1 cut(s) 373
ScrFI CCNGG 3 cut(s) 394, 712, 794
SduI GDGCHC 3 cut(s) 72, 121, 589
SfcI CTRYAG 1 cut(s) 79
SinI GGWCC 2 cut(s) 404, 992
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
Sse9I AATT 5 cut(s) 10, 106, 423, 462, 986
SseBI AGGCCT 1 cut(s) 455
SspMI CTAG 3 cut(s) 140, 197, 302
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 3 cut(s) 392, 710, 792
StyI CCWWGG 1 cut(s) 602
TaaI ACNGT 2 cut(s) 65, 961
TaiI ACGT 1 cut(s) 391
TaqI TCGA 1 cut(s) 273
TasI AATT 5 cut(s) 10, 106, 423, 462, 986
TatI WGTACW 1 cut(s) 739
TfiI GAWTC 4 cut(s) 35, 245, 785, 850
Tru1I TTAA 6 cut(s) 422, 632, 657, 666, 768, 997
Tru9I TTAA 6 cut(s) 422, 632, 657, 666, 768, 997
TseI GCWGC 3 cut(s) 470, 598, 826
TspDTI ATGAA 2 cut(s) 18, 903
TspGWI ACGGA 1 cut(s) 592
Vha464I CTTAAG 1 cut(s) 665
VneI GTGCAC 1 cut(s) 117
VpaK11BI GGWCC 2 cut(s) 404, 992
VspI ATTAAT 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 903
XmnI GAANNNNTTC 1 cut(s) 94
XspI CTAG 3 cut(s) 140, 197, 302
ZrmI AGTACT 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.