Rh5CG322100

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000487
Physical Location & Seq
Reverse (-)
35605290 .. 35612826
7537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG322100.1

Sequence Viewer

Length: 1350 bp
ATGATGATTCATGAAAACTATTGCTATGTTGTAAATTTTGCCAACTTTGGAGCTAAACCAGGAATCCAGGATATATCATTGAACTTAAAAACATTAGTTGCACATCCTGCTTGCCGAAGAATGATGGAAAACACAGGGCTTCAGGGAGAAGTTCCTCAAGCTCTCTTCAGCTTTGGGAATTTGGAGACAGTGATACTGCGGAACAACGTTCTCAGTGGCTTATTGGATGTTGGAAACTCCAGCAACCAGTTGCAACTAATTGATCTGCAGAAGAACTTCATTACTGAACTTGCACCAAACAAGGAAGGATCCAACTACACATTGATACTGGTTAATAATCCAATTTGTGAGGGGAGTCAGACTAATAATTACTGCATTGTTTCTCCAACTAATGCACCAAACGAAATGTCACCAACTCTAAATAACTGTACACCTGCTGCTTGTCCTTCAGGCCAGGTTTCTTCCCCTAACTGTAAATGTGCATCTCCCTACAGAGGAACTCTAGTCTTCATATTTGTTTCTTTCTCAAATTTGGGAAACTTTAGTTATTACAGAGAGCTTGAGAACGCTATCATGAAAAGTTGTCAGTCCTTTAACCTCCCCGTGGATTCAGTTTCTCTGGGTCGTCCTTCCTGGGGTTCATCTTTTCATCTTGAGCTAGTCATAGAAGTCTTCCCATCTGGTGATCAAGTGCGCTTCAACCAAACAGGAGCTTCAGCAATTGCTTCTGTTTTAAGCAACCAGACTCTCGAAGGGCGCCCAATATTTTTTGGACCCTACTCTGTGTTTTATCCTTCCGGTAGCTCTGGAGGATCAAATAAGGGACTGATAATTGGTGCATCCATTGGTGGTTCTGTGCTTCTGTTATTAATAGTCCTTGTAGGGTTTTATGCTCTCTACCAAAAGAGGAGAGGAGACTCGCCATCACGTGGATCAATCCTCATGCAGACAGGGGTTTCAAATGTCAGTGGTCCACAGTTAAAAGGAGCAAGGTTGTTCTCTTTTGAAGAGCTGAAGAAATATACAAACGGTTTTTCAGAAGAAAATGATATTGGATCTGGGGGTTATGGCCAGGTGTATCGGGGAACTCTTCCTATGGGCCAAACGGTTGCCATTAAAAGAGCTAAACGGGAATCTATGCAAGGTGCGCCTGAGTTCAAAGCCGAGGTTGAGCTTCTATCAAGAGTACACCATAAGAATCTTGTCAGCCTTGTTGGTTTTTGTTTGGACCAAGGTGAACAAATGTTGGTATATGAGTATGTTCCAAATGGTGATCTGCTGGATAGTCTTTTAGGTATTTCATGCAGTTCTTTACCTTCATTTATGAATTGTAGGACTTTGAGGCTTTAA

Protein Analysis

449

Amino Acids

48.76

Weight (kDa)

6.93

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 346 - 430 9.6e-13 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 348 - 430 3.2e-16 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49760 AT5G49770
fragaria_vesca FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23881 FvH4_3g23881 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_5g27161 FvH4_5g27170
malus_domestica MD11G1206300.v1.1
prunus_persica Prupe.4G230700_v2.0.a1 Prupe.4G230700_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1
pyrus_communis pycom11g17930
rosa_chinensis RchiOBHm_Chr5g0042211 RchiOBHm_Chr5g0042261 RchiOBHm_Chr5g0042271 RchiOBHm_Chr5g0042311 RchiOBHm_Chr5g0042381 RchiOBHm_Chr7g0218261
rosa_laevigata RLG00000002384 RLG00000002385 RLG00000034112 RLG00000034114 RLG00000034116 RLG00000034118
rosa_multiflora Rmu_sc0001715.1_g000008 Rmu_sc0001715.1_g000012 Rmu_sc0001715.1_g000026 Rmu_sc0004956.1_g000008
rosa_roxburghii Rroxscaffold_1G00038360 Rroxscaffold_1G00038390 Rroxscaffold_1G00038400 Rroxscaffold_1G00038450 Rroxscaffold_3G00241180
rosa_rugosa Rorug05G0198800 Rorug05G0199000 Rorug05G0199100.1 Rorug05G0199200 Rorug05G0199300 Rorug07G0175900
rosa_samantha Rh5AG283400 Rh5AG283900 Rh5AG284100 Rh5AG284300 Rh5AG284900 Rh5AG285300 Rh5AG285700 Rh5BG289300 Rh5BG289900 Rh5BG290200 Rh5BG290400 Rh5CG320700 Rh5CG321200 Rh5CG321500 Rh5CG322100 Rh5CG322400 Rh5DG298600 Rh5DG299100 Rh5DG299400 Rh5DG300200 Rh7AG317500 Rh7BG308900 Rh7CG334400 Rh7DG316500
rosa_wichuraiana Rw0G001480 Rw0G001500 Rw0G001750 Rw0G001780 Rw0G015430 Rw5G026520 Rw7G026960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 442
Acc36I ACCTGC 1 cut(s) 442
AccB1I GGYRCC 1 cut(s) 756
AccB7I CCANNNNNTGG 1 cut(s) 929
AciI CCGC 1 cut(s) 199
AclI AACGTT 1 cut(s) 207
AclWI GGATC 5 cut(s) 303, 316, 820, 940, 1063
AcoI YGGCCR 1 cut(s) 1069
AcsI RAATTY 3 cut(s) 34, 178, 529
AcuI CTGAAG 5 cut(s) 125, 151, 432, 699, 1034
AcvI CACGTG 1 cut(s) 929
AcyI GRCGYC 1 cut(s) 757
AfaI GTAC 2 cut(s) 430, 1188
AfiI CCNNNNNNNGG 4 cut(s) 494, 604, 635, 929
AgsI TTSAA 5 cut(s) 82, 700, 960, 1007, 1159
AjnI CCWGG 5 cut(s) 58, 66, 453, 632, 1071
AjuI GAANNNNNNNTTGG 2 cut(s) 1035, 1067
Alw26I GTCTC 2 cut(s) 179, 909
AlwI GGATC 5 cut(s) 303, 316, 820, 940, 1063
AoxI GGCC 3 cut(s) 451, 1069, 1099
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 3 cut(s) 34, 178, 529
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AseI ATTAAT 1 cut(s) 869
Asp700I GAANNNNTTC 1 cut(s) 275
AspLEI GCGC 3 cut(s) 696, 759, 1150
AspS9I GGNCC 4 cut(s) 773, 971, 1099, 1228
AsuHPI GGTGA 4 cut(s) 402, 695, 1247, 1283
AvaII GGWCC 3 cut(s) 773, 971, 1228
BalI TGGCCA 1 cut(s) 1071
BamHI GGATCC 1 cut(s) 308
BanI GGYRCC 1 cut(s) 756
BbrPI CACGTG 1 cut(s) 929
BbsI GAAGAC 2 cut(s) 499, 664
BbvI GCAGC 1 cut(s) 424
BccI CCATC 3 cut(s) 118, 685, 931
BciT130I CCWGG 5 cut(s) 60, 68, 455, 634, 1073
BclI TGATCA 1 cut(s) 685
BcoDI GTCTC 2 cut(s) 179, 909
BfaI CTAG 2 cut(s) 503, 659
BfmI CTRYAG 2 cut(s) 266, 490
BfoI RGCGCY 1 cut(s) 760
BfuAI ACCTGC 1 cut(s) 442
BisI GCNGC 1 cut(s) 438
BlsI GCNGC 1 cut(s) 439
Bme1390I CCNGG 5 cut(s) 60, 68, 455, 634, 1073
Bme18I GGWCC 3 cut(s) 773, 971, 1228
BmgT120I GGNCC 4 cut(s) 773, 971, 1099, 1228
BmiI GGNNCC 3 cut(s) 310, 758, 775
BmrFI CCNGG 5 cut(s) 60, 68, 455, 634, 1073
BmsI GCATC 2 cut(s) 491, 848
BpiI GAAGAC 2 cut(s) 499, 664
BpmI CTGGAG 2 cut(s) 223, 828
BpuEI CTTGAG 3 cut(s) 141, 581, 674
BsaAI YACGTR 1 cut(s) 929
BsaHI GRCGYC 1 cut(s) 757
BsaJI CCNNGG 4 cut(s) 603, 633, 1164, 1231
BsaWI WCCGGW 1 cut(s) 797
Bsc4I CCNNNNNNNGG 4 cut(s) 494, 604, 635, 929
Bse1I ACTGG 2 cut(s) 247, 333
BseBI CCWGG 5 cut(s) 60, 68, 455, 634, 1073
BseDI CCNNGG 4 cut(s) 603, 633, 1164, 1231
BseGI GGATG 3 cut(s) 103, 232, 839
BseLI CCNNNNNNNGG 4 cut(s) 494, 604, 635, 929
BseMII CTCAG 2 cut(s) 226, 1143
BseNI ACTGG 2 cut(s) 247, 333
BseRI GAGGAG 2 cut(s) 922, 927
BseXI GCAGC 1 cut(s) 424
BshFI GGCC 3 cut(s) 453, 1071, 1101
BshNI GGYRCC 1 cut(s) 756
BsiSI CCGG 1 cut(s) 798
BslFI GGGAC 1 cut(s) 837
BslI CCNNNNNNNGG 4 cut(s) 494, 604, 635, 929
BsmAI GTCTC 2 cut(s) 179, 909
BsmFI GGGAC 1 cut(s) 837
BsnI GGCC 3 cut(s) 453, 1071, 1101
Bsp1407I TGTACA 1 cut(s) 428
Bsp143I GATC 7 cut(s) 262, 308, 685, 812, 932, 1055, 1273
BspACI CCGC 1 cut(s) 199
BspANI GGCC 3 cut(s) 453, 1071, 1101
BspCNI CTCAG 2 cut(s) 225, 1144
BspHI TCATGA 2 cut(s) 10, 573
BspLI GGNNCC 3 cut(s) 310, 758, 775
BspMAI CTGCAG 1 cut(s) 270
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 5 cut(s) 303, 316, 820, 940, 1063
BspQI GCTCTTC 1 cut(s) 1002
BspT107I GGYRCC 1 cut(s) 756
BsrGI TGTACA 1 cut(s) 428
BsrI ACTGG 2 cut(s) 247, 333
BssECI CCNNGG 4 cut(s) 603, 633, 1164, 1231
BssMI GATC 7 cut(s) 262, 308, 685, 812, 932, 1055, 1273
BssNI GRCGYC 1 cut(s) 757
BssT1I CCWWGG 1 cut(s) 1231
Bst2UI CCWGG 5 cut(s) 60, 68, 455, 634, 1073
Bst4CI ACNGT 6 cut(s) 190, 428, 473, 978, 1031, 1108
Bst6I CTCTTC 3 cut(s) 170, 1002, 1095
BstACI GRCGYC 1 cut(s) 757
BstAPI GCANNNNNTGC 1 cut(s) 107
BstAUI TGTACA 1 cut(s) 428
BstBAI YACGTR 1 cut(s) 929
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 2 cut(s) 212, 1152
BstDSI CCRYGG 1 cut(s) 603
BstF5I GGATG 3 cut(s) 103, 232, 839
BstH2I RGCGCY 1 cut(s) 760
BstHHI GCGC 3 cut(s) 696, 759, 1150
BstKTI GATC 7 cut(s) 265, 311, 688, 815, 935, 1058, 1276
BstMAI GTCTC 2 cut(s) 179, 909
BstMBI GATC 7 cut(s) 262, 308, 685, 812, 932, 1055, 1273
BstMWI GCNNNNNNNGC 2 cut(s) 107, 1147
BstNI CCWGG 5 cut(s) 60, 68, 455, 634, 1073
BstSCI CCNGG 5 cut(s) 58, 66, 453, 632, 1071
BstSFI CTRYAG 2 cut(s) 266, 490
BstV1I GCAGC 1 cut(s) 424
BstV2I GAAGAC 2 cut(s) 499, 664
BstX2I RGATCY 2 cut(s) 308, 1055
BstYI RGATCY 2 cut(s) 308, 1055
BsuRI GGCC 3 cut(s) 453, 1071, 1101
BtgI CCRYGG 1 cut(s) 603
BtsCI GGATG 3 cut(s) 103, 232, 839
BtsIMutI CAGTG 3 cut(s) 195, 220, 973
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 112
CciI TCATGA 2 cut(s) 10, 573
CfoI GCGC 3 cut(s) 696, 759, 1150
Cfr13I GGNCC 4 cut(s) 773, 971, 1099, 1228
Csp6I GTAC 2 cut(s) 429, 1187
CviAII CATG 4 cut(s) 11, 574, 943, 1302
CviQI GTAC 2 cut(s) 429, 1187
DdeI CTNAG 2 cut(s) 212, 1152
DinI GGCGCC 1 cut(s) 758
DpnI GATC 7 cut(s) 264, 310, 687, 814, 934, 1057, 1275
DpnII GATC 7 cut(s) 262, 308, 685, 812, 932, 1055, 1273
EaeI YGGCCR 1 cut(s) 1069
Eam1104I CTCTTC 3 cut(s) 170, 1002, 1095
EarI CTCTTC 3 cut(s) 170, 1002, 1095
Eco130I CCWWGG 1 cut(s) 1231
Eco47I GGWCC 3 cut(s) 773, 971, 1228
Eco57I CTGAAG 5 cut(s) 125, 151, 432, 699, 1034
Eco72I CACGTG 1 cut(s) 929
EcoRII CCWGG 5 cut(s) 58, 66, 453, 632, 1071
EcoT14I CCWWGG 1 cut(s) 1231
EgeI GGCGCC 1 cut(s) 758
EheI GGCGCC 1 cut(s) 758
ErhI CCWWGG 1 cut(s) 1231
FaeI CATG 4 cut(s) 14, 577, 946, 1305
FaqI GGGAC 1 cut(s) 837
FatI CATG 4 cut(s) 10, 573, 942, 1301
FbaI TGATCA 1 cut(s) 685
Fnu4HI GCNGC 1 cut(s) 438
FokI GGATG 3 cut(s) 90, 239, 826
Fsp4HI GCNGC 1 cut(s) 438
FspBI CTAG 2 cut(s) 503, 659
GlaI GCGC 3 cut(s) 695, 758, 1149
GluI GCNGC 1 cut(s) 438
GsuI CTGGAG 2 cut(s) 223, 828
HaeII RGCGCY 1 cut(s) 760
HaeIII GGCC 3 cut(s) 453, 1071, 1101
HapII CCGG 1 cut(s) 798
HhaI GCGC 3 cut(s) 696, 759, 1150
Hin1I GRCGYC 1 cut(s) 757
Hin1II CATG 4 cut(s) 14, 577, 946, 1305
Hin6I GCGC 3 cut(s) 694, 757, 1148
HinP1I GCGC 3 cut(s) 694, 757, 1148
HinfI GANTC 8 cut(s) 7, 63, 355, 608, 745, 917, 1133, 1198
HpaII CCGG 1 cut(s) 798
HphI GGTGA 4 cut(s) 402, 695, 1247, 1283
Hpy166II GTNNAC 4 cut(s) 431, 974, 1189, 1238
Hpy188I TCNGA 2 cut(s) 360, 1039
Hpy188III TCNNGA 6 cut(s) 11, 574, 653, 749, 807, 1182
Hpy8I GTNNAC 4 cut(s) 431, 974, 1189, 1238
HpyAV CCTTC 6 cut(s) 299, 456, 639, 746, 804, 1326
HpyCH4III ACNGT 6 cut(s) 190, 428, 473, 978, 1031, 1108
HpyCH4IV ACGT 2 cut(s) 207, 928
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 1147
HpyF3I CTNAG 2 cut(s) 212, 1152
HpySE526I ACGT 2 cut(s) 207, 928
Hsp92I GRCGYC 1 cut(s) 757
Hsp92II CATG 4 cut(s) 14, 577, 946, 1305
HspAI GCGC 3 cut(s) 694, 757, 1148
KasI GGCGCC 1 cut(s) 756
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 7 cut(s) 262, 308, 685, 812, 932, 1055, 1273
LguI GCTCTTC 1 cut(s) 1002
LmnI GCTCC 3 cut(s) 50, 710, 986
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 2 cut(s) 491, 848
MaeI CTAG 2 cut(s) 503, 659
MaeII ACGT 2 cut(s) 207, 928
MaeIII GTNAC 1 cut(s) 408
MalI GATC 7 cut(s) 264, 310, 687, 814, 934, 1057, 1275
MboI GATC 7 cut(s) 262, 308, 685, 812, 932, 1055, 1273
MfeI CAATTG 1 cut(s) 720
MflI RGATCY 2 cut(s) 308, 1055
MlsI TGGCCA 1 cut(s) 1071
MluCI AATT 9 cut(s) 34, 178, 258, 342, 367, 529, 720, 831, 1327
MluNI TGGCCA 1 cut(s) 1071
Mly113I GGCGCC 1 cut(s) 757
MlyI GAGTC 3 cut(s) 364, 739, 911
MmeI TCCRAC 3 cut(s) 211, 336, 410
Mox20I TGGCCA 1 cut(s) 1071
MroXI GAANNNNTTC 1 cut(s) 275
MscI TGGCCA 1 cut(s) 1071
MseI TTAA 8 cut(s) 86, 333, 594, 734, 869, 980, 1116, 1348
Msp20I TGGCCA 1 cut(s) 1071
MspI CCGG 1 cut(s) 798
MspR9I CCNGG 5 cut(s) 60, 68, 455, 634, 1073
MunI CAATTG 1 cut(s) 720
MvaI CCWGG 5 cut(s) 60, 68, 455, 634, 1073
MwoI GCNNNNNNNGC 2 cut(s) 107, 1147
NarI GGCGCC 1 cut(s) 757
NdeII GATC 7 cut(s) 262, 308, 685, 812, 932, 1055, 1273
NlaIII CATG 4 cut(s) 14, 577, 946, 1305
NlaIV GGNNCC 3 cut(s) 310, 758, 775
NmeAIII GCCGAG 1 cut(s) 1189
NmuCI GTSAC 1 cut(s) 408
PagI TCATGA 2 cut(s) 10, 573
PaqCI CACCTGC 1 cut(s) 442
PciSI GCTCTTC 1 cut(s) 1002
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 5 cut(s) 7, 63, 608, 1133, 1198
PflMI CCANNNNNTGG 1 cut(s) 929
PfoI TCCNGGA 1 cut(s) 66
PkrI GCNGC 1 cut(s) 439
PleI GAGTC 3 cut(s) 363, 739, 911
PluTI GGCGCC 1 cut(s) 760
PmaCI CACGTG 1 cut(s) 929
PmlI CACGTG 1 cut(s) 929
PpsI GAGTC 3 cut(s) 363, 739, 911
Ppu21I YACGTR 1 cut(s) 929
PshBI ATTAAT 1 cut(s) 869
Psp1406I AACGTT 1 cut(s) 207
Psp6I CCWGG 5 cut(s) 58, 66, 453, 632, 1071
PspCI CACGTG 1 cut(s) 929
PspGI CCWGG 5 cut(s) 58, 66, 453, 632, 1071
PspN4I GGNNCC 3 cut(s) 310, 758, 775
PspPI GGNCC 4 cut(s) 773, 971, 1099, 1228
PstI CTGCAG 1 cut(s) 270
PsuI RGATCY 2 cut(s) 308, 1055
RsaI GTAC 2 cut(s) 430, 1188
RsaNI GTAC 2 cut(s) 429, 1187
SapI GCTCTTC 1 cut(s) 1002
SaqAI TTAA 8 cut(s) 86, 333, 594, 734, 869, 980, 1116, 1348
SatI GCNGC 1 cut(s) 438
Sau3AI GATC 7 cut(s) 262, 308, 685, 812, 932, 1055, 1273
Sau96I GGNCC 4 cut(s) 773, 971, 1099, 1228
SchI GAGTC 3 cut(s) 364, 739, 911
ScrFI CCNGG 5 cut(s) 60, 68, 455, 634, 1073
SfaNI GCATC 2 cut(s) 491, 848
SfcI CTRYAG 2 cut(s) 266, 490
SfoI GGCGCC 1 cut(s) 758
SinI GGWCC 3 cut(s) 773, 971, 1228
SmlI CTYRAG 3 cut(s) 156, 560, 653
SmoI CTYRAG 3 cut(s) 156, 560, 653
Sse9I AATT 9 cut(s) 34, 178, 258, 342, 367, 529, 720, 831, 1327
SsiI CCGC 1 cut(s) 199
SspDI GGCGCC 1 cut(s) 756
SspI AATATT 1 cut(s) 765
SspMI CTAG 2 cut(s) 503, 659
StyD4I CCNGG 5 cut(s) 58, 66, 453, 632, 1071
StyI CCWWGG 1 cut(s) 1231
TaaI ACNGT 6 cut(s) 190, 428, 473, 978, 1031, 1108
TaiI ACGT 2 cut(s) 210, 931
TaqI TCGA 1 cut(s) 750
TasI AATT 9 cut(s) 34, 178, 258, 342, 367, 529, 720, 831, 1327
TatI WGTACW 2 cut(s) 428, 1186
TfiI GAWTC 5 cut(s) 7, 63, 608, 1133, 1198
Tru1I TTAA 8 cut(s) 86, 333, 594, 734, 869, 980, 1116, 1348
Tru9I TTAA 8 cut(s) 86, 333, 594, 734, 869, 980, 1116, 1348
TscAI CASTG 3 cut(s) 195, 220, 973
TseFI GTSAC 1 cut(s) 408
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 1 cut(s) 408
TspDTI ATGAA 9 cut(s) 27, 268, 499, 590, 630, 638, 1290, 1308, 1340
TspRI CASTG 3 cut(s) 195, 220, 973
Van91I CCANNNNNTGG 1 cut(s) 929
VpaK11BI GGWCC 3 cut(s) 773, 971, 1228
VspI ATTAAT 1 cut(s) 869
XapI RAATTY 3 cut(s) 34, 178, 529
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 2 cut(s) 503, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.