FvH4_5g27170

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000487
Physical Location & Seq
Forward (+)
18439009 .. 18445742
6734 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27170.t1

Sequence Viewer

Length: 2889 bp
ATGGATCACAGAACTCAGGTGTTTCTGCTTCTTATATTCGCTCCTTTTCTCGTTTTAGCCAATCATGTGACAAGTCCTCAGGATGTTATGGGTTTGACGTTTCTAAAGCAACTATGGAATAACACGCCTCCAAGTTGGGGGACTCGTCTGTCTGATCCTTGTGAAGATGACTGGGATGGCATTAGGTGTACCAACATGCGTGTGATCTCTTTAATATTACCAAGTATGAATTTGACTGGCACACTCTCTGGAGATATTGATCAGTTTTCTGAACTACAAATTTTGGATCTATCTTACAACAAGGGCCTGACAGGACCACTCCCAACAGAAATTGGGAGCTTGAAGAAGCTGTATAACATAATCCTGGTTGGTTGCAGTTTCTCTGGTCTCATCCCTGCTTCAATAGGGTCCCTACAAGAGCTAATTTTTCTATCTCTGAATTCTAACAGATTTAGTGGGAGGATCCCATCTTCCATTGGTAATCTGTCTAAACTGTACTGGCTGGATCTAGCTGACAACAACCTCGAAGGACCCATCCCAGTTTCTGATGGTGTTGAACCTGGACTTGACATGCTCAGTCATTGCAAGCACTTTCATCTCGGAATCAATCATCTCTCAGGCCCAATTCCATCAAAGCTTTTTAACTCAAACATGAGACTGATACATGTGCTCTTCGAAAGTAATAAACTCACTGGCAGCATCCCTTCCAGCGTTGGACTTGTAAAGTCTTTGGAGGTCGTGCGATTTGATGATAACATATTAAGTGGGCTTATTCCTCTCAGCTTCAACAATCTTACAAATGTTTCTCAGCTGTTCTTGTCCAACAACAAGTTGAGCGGCCCTGCTCCCGACCTTACTGGCATGATCGGTCTCCATTATGTGGATTTGAGCAATAATAGTTTCGACACGTCTGATGTTCCACCATGGTTCTCAACACTAGAGTCTTTGACCACATTAATCATGGAGGACACAAAACTTCAAGGAGAAATACCTGTTTCTCTATTCAGCCTTTCAAACTTACAGACTGTGGTCCTGAAGAACAACCAGCTAAATGGCACCTTGGATATTGGGACTTCCTATAGCAGTCAATTGCAACTCATTGATTTACAAAATAATAAAATTCATGAATACGAGGGAAATGGAGGATATGATGGCATTTTAATACTTGTTCAAAATCCAGTTTGTACTGAAACTGGGGCATCACTGATGAGTTACTGCAAGATTTCACAATCATCTGATCTCGTCCAATATGAAACATTGCCAAATAACTGTGTTCCCAGTGCTTGCAGTTCCGATCAGGTTTCAAGCCCCAACTGTAAATGTGCATATCCATACGGGGGCAGAATAAACTTTAGAGCTCCTACCTTCTCAGACTGGGAAAACTCAAATGTCTTCAAAGAGCTTGAGAACAATCTCACGGGTTTTTTCAGTAAACATCAAGTTAACGTTGATTCAGTCTCTTTCAGTAACCTCACCAAGGACTCCCATGCATACATTTGGTTGAGCCTAGAAGTATTCCCACAAATCCAAAATCGTTTCACTAGAAAGGAAGTTTCTAATATTGCTTTTCTACTCAGTAACCAAACATTCAAGCCTCAAAAGTATTTTGGACCAATCGTTTTTATCGGAAATGATTATTCAGACTTTGCAGTAGAGACGATAGCACCATCAAAACCAAACAAATCGTCCAATGTGGGCATTATCATTGGAGCAGCAGCTGGTGCTTCTGTTCTTGTGCTGATATTTCTCCTTGTAGGAATTTATGCTATTCGCCAGAAGCGAAGGGCAAAAAGAGCCACTGATCAAAACAATCCTTTTGGCAAATGGGATGTGAATAATAAAAGCAGTGGTAGCATTCCTCAGTTAAAAGGTGCTAGATCGTTCTCTTTTGAAGAGCTTATGAAATATAGCAACAACTTTTCAGAAGCAAATGATGTTGGATCTGGGGGATATGGGAAGGTTTACCGTGGAACTCTTCCCACAGGGCAACTGATTGCCATCAAACGAGCTCACACAGAATCTATCCAAGGTGGACATCAGTTCAAAAGTGAGATTGAACTTCTTTCACGAGTTCATCATAAGAACCTTGTCAGCCTTGTAGGATTTTGTTTTGACCGAGATGAGCAGATGCTGGTGTATGAGTATGTAGCAAATGGGACTCTTAAGGATAGTCTTTCAGGTAAGTCAGGAATCAGATTAGATTGGCCGAGGAGACTTAAAATAGCACTTGGTACAGCTAGAGGCTTAGCCTATCTTCATGAACTTTGCCACCCTCCAATTATCCACAGAGATATCAAGTCAACTAATGTTCTGTTGGACAACAGATTGAGTGCGAAAGTTGCTGATTTTGGTCTCTCCAAGTCCATGGGTGACAGTGGGAGGGATCATGTCTCCACTCAAGTTAAAGGGACAATGGGATACATGGATCCTGAATATTACATGACTCAACGGTTGACTGAGAAGAGTGATGTTTATGGTTTTGGAGTGCTAATGTTGGAGATACTTACTGCAAGAAAACCGATAGAACAAGGAAAATATATCGTGCGAGAAGTAAGAATGGCAATGGACAGGACTAAAGAATTATACAACCTTCATGTGATCCTGGATTCAGCCATTGGTTTGAGAACAACACTGGTAGGTTTAGAGAGGTTTGTGGATCTTGCAATGGCTTGTGTTCAAGAAGAAGGAGTCAACAGGCCTACAATGAGTGAGGTGGTGAAAGAGATTGAATACATTATGCAGCTTGCTGGACTGAACCCTAATGCTGAATCGGCATCCACTTCCTCAAGCTATGAGGATCCGGCAAGGGTTAGGCATCCGTACAGCAATATTGAGGCTGCCTTTGATTACAGTGGAGTGTTTCCGTCTTCAAAGATAGAGCCGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 Pkinase Pkinase_Tyr

Protein Analysis

963

Amino Acids

106.47

Weight (kDa)

6.06

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 84 - 170 1.2e-06 Leucine-rich repeat region
Pkinase PF00069 642 - 907 2.9e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 644 - 908 1.7e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49760 AT5G49770
fragaria_vesca FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23881 FvH4_3g23881 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_5g27161 FvH4_5g27170
malus_domestica MD11G1206300.v1.1
prunus_persica Prupe.4G230700_v2.0.a1 Prupe.4G230700_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1
pyrus_communis pycom11g17930
rosa_chinensis RchiOBHm_Chr5g0042211 RchiOBHm_Chr5g0042261 RchiOBHm_Chr5g0042271 RchiOBHm_Chr5g0042311 RchiOBHm_Chr5g0042381 RchiOBHm_Chr7g0218261
rosa_laevigata RLG00000002384 RLG00000002385 RLG00000034112 RLG00000034114 RLG00000034116 RLG00000034118
rosa_multiflora Rmu_sc0001715.1_g000008 Rmu_sc0001715.1_g000012 Rmu_sc0001715.1_g000026 Rmu_sc0004956.1_g000008
rosa_roxburghii Rroxscaffold_1G00038360 Rroxscaffold_1G00038390 Rroxscaffold_1G00038400 Rroxscaffold_1G00038450 Rroxscaffold_3G00241180
rosa_rugosa Rorug05G0198800 Rorug05G0199000 Rorug05G0199100.1 Rorug05G0199200 Rorug05G0199300 Rorug07G0175900
rosa_samantha Rh5AG283400 Rh5AG283900 Rh5AG284100 Rh5AG284300 Rh5AG284900 Rh5AG285300 Rh5AG285700 Rh5BG289300 Rh5BG289900 Rh5BG290200 Rh5BG290400 Rh5CG320700 Rh5CG321200 Rh5CG321500 Rh5CG322100 Rh5CG322400 Rh5DG298600 Rh5DG299100 Rh5DG299400 Rh5DG300200 Rh7AG317500 Rh7BG308900 Rh7CG334400 Rh7DG316500
rosa_wichuraiana Rw0G001480 Rw0G001500 Rw0G001750 Rw0G001780 Rw0G015430 Rw5G026520 Rw7G026960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 148
AccB1I GGYRCC 1 cut(s) 1055
AccB7I CCANNNNNTGG 1 cut(s) 880
AccBSI CCGCTC 1 cut(s) 837
AciI CCGC 2 cut(s) 837, 2882
AclI AACGTT 1 cut(s) 1446
AcoI YGGCCR 1 cut(s) 2204
AcsI RAATTY 5 cut(s) 229, 279, 439, 1119, 1758
AcuI CTGAAG 1 cut(s) 1055
AfaI GTAC 5 cut(s) 190, 497, 1186, 2233, 2822
AfiI CCNNNNNNNGG 4 cut(s) 137, 880, 1337, 1477
AflII CTTAAG 1 cut(s) 2162
AflIII ACRYGT 2 cut(s) 664, 906
AjiI CACGTC 1 cut(s) 909
AjnI CCWGG 3 cut(s) 363, 559, 2601
Alw21I GWGCWC 3 cut(s) 672, 1360, 2011
Alw26I GTCTC 8 cut(s) 392, 649, 875, 1462, 1649, 2206, 2357, 2395
AlwNI CAGNNNCTG 3 cut(s) 545, 1718, 2131
AoxI GGCC 5 cut(s) 304, 619, 838, 2204, 2696
ApeKI GCWGC 5 cut(s) 696, 1712, 1715, 2740, 2837
ApoI RAATTY 5 cut(s) 229, 279, 439, 1119, 1758
ArsI GACNNNNNNTTYG 2 cut(s) 1670, 1702
AseI ATTAAT 1 cut(s) 956
AspS9I GGNCC 8 cut(s) 304, 314, 408, 530, 620, 839, 1030, 1610
AsuHPI GGTGA 3 cut(s) 1465, 2381, 2728
AsuII TTCGAA 1 cut(s) 675
AvaII GGWCC 5 cut(s) 314, 408, 530, 1030, 1610
AxyI CCTNAGG 1 cut(s) 78
BamHI GGATCC 3 cut(s) 462, 2425, 2797
BanI GGYRCC 1 cut(s) 1055
BanII GRGCYC 2 cut(s) 1360, 2011
BauI CACGAG 1 cut(s) 2067
BbsI GAAGAC 2 cut(s) 1384, 2859
Bbv12I GWGCWC 3 cut(s) 672, 1360, 2011
BbvI GCAGC 5 cut(s) 708, 1724, 1727, 2752, 2824
BccI CCATC 8 cut(s) 170, 475, 542, 542, 637, 1145, 1675, 2006
BciT130I CCWGG 3 cut(s) 365, 561, 2603
BciVI GTATCC 1 cut(s) 2411
BclI TGATCA 2 cut(s) 259, 1801
BcoDI GTCTC 8 cut(s) 392, 649, 875, 1462, 1649, 2206, 2357, 2395
BfaI CTAG 6 cut(s) 509, 938, 1508, 1542, 1875, 2238
BfmI CTRYAG 1 cut(s) 1078
BfrI CTTAAG 1 cut(s) 2162
BfuI GTATCC 1 cut(s) 2411
BisI GCNGC 7 cut(s) 697, 838, 1713, 1716, 2741, 2838, 2882
BlpI GCTNAGC 1 cut(s) 2245
BlsI GCNGC 7 cut(s) 698, 839, 1714, 1717, 2742, 2839, 2883
Bme1390I CCNGG 3 cut(s) 365, 561, 2603
Bme18I GGWCC 5 cut(s) 314, 408, 530, 1030, 1610
BmgBI CACGTC 1 cut(s) 909
BmgT120I GGNCC 8 cut(s) 304, 314, 408, 530, 620, 839, 1030, 1610
BmiI GGNNCC 7 cut(s) 409, 410, 464, 532, 1057, 2427, 2799
BmrFI CCNGG 3 cut(s) 365, 561, 2603
BmrI ACTGGG 5 cut(s) 181, 533, 1203, 1272, 1384
BmsI GCATC 5 cut(s) 708, 1208, 2118, 2783, 2824
BmuI ACTGGG 5 cut(s) 181, 533, 1203, 1272, 1384
BoxI GACNNNNGTC 1 cut(s) 1028
BpiI GAAGAC 2 cut(s) 1384, 2859
BpmI CTGGAG 1 cut(s) 270
Bpu1102I GCTNAGC 1 cut(s) 2245
Bpu14I TTCGAA 1 cut(s) 675
BpuEI CTTGAG 3 cut(s) 1424, 2382, 2770
BsaBI GATNNNNATC 2 cut(s) 258, 1997
BsaI GGTCTC 3 cut(s) 392, 875, 2357
BsaJI CCNNGG 7 cut(s) 923, 1059, 1476, 1966, 2026, 2207, 2364
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 880, 1337, 1477
Bse21I CCTNAGG 1 cut(s) 78
Bse3DI GCAATG 4 cut(s) 580, 1256, 2568, 2670
Bse8I GATNNNNATC 2 cut(s) 258, 1997
BseBI CCWGG 3 cut(s) 365, 561, 2603
BseDI CCNNGG 7 cut(s) 923, 1059, 1476, 1966, 2026, 2207, 2364
BseGI GGATG 8 cut(s) 88, 181, 390, 534, 699, 1834, 2774, 2815
BseJI GATNNNNATC 2 cut(s) 258, 1997
BseLI CCNNNNNNNGG 4 cut(s) 137, 880, 1337, 1477
BseMI GCAATG 4 cut(s) 580, 1256, 2568, 2670
BseRI GAGGAG 1 cut(s) 2224
BseXI GCAGC 5 cut(s) 708, 1724, 1727, 2752, 2824
BshFI GGCC 5 cut(s) 306, 621, 840, 2206, 2698
BshNI GGYRCC 1 cut(s) 1055
BsiHKAI GWGCWC 3 cut(s) 672, 1360, 2011
BsiSI CCGG 1 cut(s) 2801
BslFI GGGAC 5 cut(s) 154, 394, 1084, 2170, 2422
BslI CCNNNNNNNGG 4 cut(s) 137, 880, 1337, 1477
BsmAI GTCTC 8 cut(s) 392, 649, 875, 1462, 1649, 2206, 2357, 2395
BsmBI CGTCTC 1 cut(s) 1649
BsmFI GGGAC 5 cut(s) 154, 394, 1084, 2170, 2422
BsmI GAATGC 1 cut(s) 1854
BsnI GGCC 5 cut(s) 306, 621, 840, 2206, 2698
Bso31I GGTCTC 3 cut(s) 392, 875, 2357
Bsp119I TTCGAA 1 cut(s) 675
Bsp1286I GDGCHC 3 cut(s) 672, 1360, 2011
Bsp1720I GCTNAGC 1 cut(s) 2245
Bsp19I CCATGG 2 cut(s) 923, 2364
BspACI CCGC 2 cut(s) 837, 2882
BspANI GGCC 5 cut(s) 306, 621, 840, 2206, 2698
BspHI TCATGA 2 cut(s) 1123, 2257
BspLI GGNNCC 7 cut(s) 409, 410, 464, 532, 1057, 2427, 2799
BspQI GCTCTTC 2 cut(s) 677, 1887
BspT104I TTCGAA 1 cut(s) 675
BspT107I GGYRCC 1 cut(s) 1055
BspTI CTTAAG 1 cut(s) 2162
BspTNI GGTCTC 3 cut(s) 392, 875, 2357
BsrBI CCGCTC 1 cut(s) 837
BsrDI GCAATG 4 cut(s) 580, 1256, 2568, 2670
BssECI CCNNGG 7 cut(s) 923, 1059, 1476, 1966, 2026, 2207, 2364
BssSI CACGAG 1 cut(s) 2067
BssT1I CCWWGG 5 cut(s) 923, 1059, 1476, 2026, 2364
Bst2BI CACGAG 1 cut(s) 2067
Bst2UI CCWGG 3 cut(s) 365, 561, 2603
Bst4CI ACNGT 8 cut(s) 495, 1027, 1271, 1316, 1967, 2375, 2451, 2852
Bst6I CTCTTC 4 cut(s) 677, 1887, 1980, 2456
BstAFI CTTAAG 1 cut(s) 2162
BstAPI GCANNNNNTGC 1 cut(s) 1721
BstBI TTCGAA 1 cut(s) 675
BstC8I GCNNGC 3 cut(s) 587, 1285, 2745
BstDSI CCRYGG 3 cut(s) 923, 1966, 2364
BstENI CCTNNNNNAGG 1 cut(s) 1475
BstF5I GGATG 8 cut(s) 88, 181, 390, 534, 699, 1834, 2774, 2815
BstMAI GTCTC 8 cut(s) 392, 649, 875, 1462, 1649, 2206, 2357, 2395
BstMWI GCNNNNNNNGC 5 cut(s) 1721, 1793, 1851, 2339, 2771
BstNI CCWGG 3 cut(s) 365, 561, 2603
BstNSI RCATGY 3 cut(s) 199, 574, 668
BstPAI GACNNNNGTC 1 cut(s) 1028
BstSCI CCNGG 3 cut(s) 363, 559, 2601
BstSFI CTRYAG 1 cut(s) 1078
BstV1I GCAGC 5 cut(s) 708, 1724, 1727, 2752, 2824
BstV2I GAAGAC 2 cut(s) 1384, 2859
BstX2I RGATCY 7 cut(s) 286, 462, 505, 1940, 2425, 2656, 2797
BstXI CCANNNNNNTGG 2 cut(s) 1052, 2365
BstYI RGATCY 7 cut(s) 286, 462, 505, 1940, 2425, 2656, 2797
Bsu36I CCTNAGG 1 cut(s) 78
BsuI GTATCC 1 cut(s) 2411
BsuRI GGCC 5 cut(s) 306, 621, 840, 2206, 2698
BtgI CCRYGG 3 cut(s) 923, 1966, 2364
BtrI CACGTC 1 cut(s) 909
BtsCI GGATG 8 cut(s) 88, 181, 390, 534, 699, 1834, 2774, 2815
BtsI GCAGTG 1 cut(s) 1852
BtsIMutI CAGTG 8 cut(s) 690, 1202, 1285, 1797, 1852, 2380, 2630, 2857
Cac8I GCNNGC 3 cut(s) 587, 1285, 2745
CaiI CAGNNNCTG 3 cut(s) 545, 1718, 2131
CciI TCATGA 2 cut(s) 1123, 2257
Cfr13I GGNCC 8 cut(s) 304, 314, 408, 530, 620, 839, 1030, 1610
Csp6I GTAC 5 cut(s) 189, 496, 1185, 2232, 2821
CviQI GTAC 5 cut(s) 189, 496, 1185, 2232, 2821
DrdI GACNNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 2204
Eam1104I CTCTTC 4 cut(s) 677, 1887, 1980, 2456
EarI CTCTTC 4 cut(s) 677, 1887, 1980, 2456
Ecl136II GAGCTC 2 cut(s) 1358, 2009
Eco130I CCWWGG 5 cut(s) 923, 1059, 1476, 2026, 2364
Eco147I AGGCCT 1 cut(s) 2698
Eco24I GRGCYC 2 cut(s) 1360, 2011
Eco31I GGTCTC 3 cut(s) 392, 875, 2357
Eco32I GATATC 1 cut(s) 2293
Eco47I GGWCC 5 cut(s) 314, 408, 530, 1030, 1610
Eco53kI GAGCTC 2 cut(s) 1358, 2009
Eco57I CTGAAG 1 cut(s) 1055
Eco81I CCTNAGG 1 cut(s) 78
EcoICRI GAGCTC 2 cut(s) 1358, 2009
EcoNI CCTNNNNNAGG 1 cut(s) 1475
EcoO109I RGGNCCY 3 cut(s) 304, 408, 530
EcoRI GAATTC 1 cut(s) 439
EcoRII CCWGG 3 cut(s) 363, 559, 2601
EcoRV GATATC 1 cut(s) 2293
EcoT14I CCWWGG 5 cut(s) 923, 1059, 1476, 2026, 2364
EcoT22I ATGCAT 1 cut(s) 1492
EcoT38I GRGCYC 2 cut(s) 1360, 2011
ErhI CCWWGG 5 cut(s) 923, 1059, 1476, 2026, 2364
Esp3I CGTCTC 1 cut(s) 1649
FaqI GGGAC 5 cut(s) 154, 394, 1084, 2170, 2422
FbaI TGATCA 2 cut(s) 259, 1801
Fnu4HI GCNGC 7 cut(s) 697, 838, 1713, 1716, 2741, 2838, 2882
FokI GGATG 8 cut(s) 95, 188, 377, 521, 686, 1841, 2761, 2802
FriOI GRGCYC 2 cut(s) 1360, 2011
Fsp4HI GCNGC 7 cut(s) 697, 838, 1713, 1716, 2741, 2838, 2882
FspBI CTAG 6 cut(s) 509, 938, 1508, 1542, 1875, 2238
GluI GCNGC 7 cut(s) 697, 838, 1713, 1716, 2741, 2838, 2882
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 5 cut(s) 306, 621, 840, 2206, 2698
HapII CCGG 1 cut(s) 2801
HincII GTYRAC 4 cut(s) 1444, 2301, 2454, 2692
HindII GTYRAC 4 cut(s) 1444, 2301, 2454, 2692
HindIII AAGCTT 1 cut(s) 635
HpaI GTTAAC 1 cut(s) 1444
HpaII CCGG 1 cut(s) 2801
HphI GGTGA 3 cut(s) 1465, 2381, 2728
Hpy166II GTNNAC 8 cut(s) 189, 1433, 1444, 1963, 2033, 2301, 2454, 2692
Hpy8I GTNNAC 8 cut(s) 189, 1433, 1444, 1963, 2033, 2301, 2454, 2692
HpyAV CCTTC 7 cut(s) 521, 714, 1375, 1776, 1951, 2600, 2678
HpyCH4III ACNGT 8 cut(s) 495, 1027, 1271, 1316, 1967, 2375, 2451, 2852
HpyCH4IV ACGT 3 cut(s) 98, 908, 1446
HpyF10VI GCNNNNNNNGC 5 cut(s) 1721, 1793, 1851, 2339, 2771
HpySE526I ACGT 3 cut(s) 98, 908, 1446
KflI GGGWCCC 1 cut(s) 408
Ksp22I TGATCA 2 cut(s) 259, 1801
KspAI GTTAAC 1 cut(s) 1444
LguI GCTCTTC 2 cut(s) 677, 1887
LmnI GCTCC 5 cut(s) 46, 336, 850, 1363, 1709
Lsp1109I GCAGC 5 cut(s) 708, 1724, 1727, 2752, 2824
LweI GCATC 5 cut(s) 708, 1208, 2118, 2783, 2824
MaeI CTAG 6 cut(s) 509, 938, 1508, 1542, 1875, 2238
MaeII ACGT 3 cut(s) 98, 908, 1446
MaeIII GTNAC 5 cut(s) 67, 1211, 1466, 1577, 2369
MbiI CCGCTC 1 cut(s) 837
MfeI CAATTG 1 cut(s) 1088
MflI RGATCY 7 cut(s) 286, 462, 505, 1940, 2425, 2656, 2797
MhlI GDGCHC 3 cut(s) 672, 1360, 2011
MlyI GAGTC 6 cut(s) 136, 950, 1475, 2152, 2437, 2697
MmeI TCCRAC 5 cut(s) 694, 846, 1918, 2295, 2475
Mph1103I ATGCAT 1 cut(s) 1492
MslI CAYNNNNRTG 1 cut(s) 200
MspA1I CMGCKG 2 cut(s) 811, 1718
MspCI CTTAAG 1 cut(s) 2162
MspI CCGG 1 cut(s) 2801
MspR9I CCNGG 3 cut(s) 365, 561, 2603
MunI CAATTG 1 cut(s) 1088
Mva1269I GAATGC 1 cut(s) 1854
MvaI CCWGG 3 cut(s) 365, 561, 2603
MwoI GCNNNNNNNGC 5 cut(s) 1721, 1793, 1851, 2339, 2771
NcoI CCATGG 2 cut(s) 923, 2364
NlaIV GGNNCC 7 cut(s) 409, 410, 464, 532, 1057, 2427, 2799
NmeAIII GCCGAG 1 cut(s) 2232
NmuCI GTSAC 2 cut(s) 67, 2369
NsiI ATGCAT 1 cut(s) 1492
NspI RCATGY 3 cut(s) 199, 574, 668
NspV TTCGAA 1 cut(s) 675
PagI TCATGA 2 cut(s) 1123, 2257
PceI AGGCCT 1 cut(s) 2698
PciI ACATGT 1 cut(s) 664
PciSI GCTCTTC 2 cut(s) 677, 1887
PcsI WCGNNNNNNNCGW 1 cut(s) 1777
PctI GAATGC 1 cut(s) 1854
PfeI GAWTC 6 cut(s) 603, 1451, 2018, 2190, 2606, 2768
PflMI CCANNNNNTGG 1 cut(s) 880
PfoI TCCNGGA 1 cut(s) 2601
PkrI GCNGC 7 cut(s) 698, 839, 1714, 1717, 2742, 2839, 2883
PleI GAGTC 6 cut(s) 136, 949, 1475, 2152, 2437, 2696
PpsI GAGTC 6 cut(s) 136, 949, 1475, 2152, 2437, 2696
PpuMI RGGWCCY 2 cut(s) 408, 530
PscI ACATGT 1 cut(s) 664
PshAI GACNNNNGTC 1 cut(s) 1028
PshBI ATTAAT 1 cut(s) 956
Psp124BI GAGCTC 2 cut(s) 1360, 2011
Psp1406I AACGTT 1 cut(s) 1446
Psp5II RGGWCCY 2 cut(s) 408, 530
Psp6I CCWGG 3 cut(s) 363, 559, 2601
PspGI CCWGG 3 cut(s) 363, 559, 2601
PspN4I GGNNCC 7 cut(s) 409, 410, 464, 532, 1057, 2427, 2799
PspPI GGNCC 8 cut(s) 304, 314, 408, 530, 620, 839, 1030, 1610
PspPPI RGGWCCY 2 cut(s) 408, 530
PstNI CAGNNNCTG 3 cut(s) 545, 1718, 2131
PsuI RGATCY 7 cut(s) 286, 462, 505, 1940, 2425, 2656, 2797
PvuII CAGCTG 2 cut(s) 811, 1718
RsaI GTAC 5 cut(s) 190, 497, 1186, 2233, 2822
RsaNI GTAC 5 cut(s) 189, 496, 1185, 2232, 2821
RseI CAYNNNNRTG 1 cut(s) 200
SacI GAGCTC 2 cut(s) 1360, 2011
SapI GCTCTTC 2 cut(s) 677, 1887
SatI GCNGC 7 cut(s) 697, 838, 1713, 1716, 2741, 2838, 2882
Sau96I GGNCC 8 cut(s) 304, 314, 408, 530, 620, 839, 1030, 1610
SchI GAGTC 6 cut(s) 136, 950, 1475, 2152, 2437, 2697
ScrFI CCNGG 3 cut(s) 365, 561, 2603
SduI GDGCHC 3 cut(s) 672, 1360, 2011
SfaNI GCATC 5 cut(s) 708, 1208, 2118, 2783, 2824
SfcI CTRYAG 1 cut(s) 1078
SfuI TTCGAA 1 cut(s) 675
SinI GGWCC 5 cut(s) 314, 408, 530, 1030, 1610
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 4 cut(s) 1403, 2162, 2397, 2785
SmoI CTYRAG 4 cut(s) 1403, 2162, 2397, 2785
SseBI AGGCCT 1 cut(s) 2698
SsiI CCGC 2 cut(s) 837, 2882
SspI AATATT 4 cut(s) 216, 1561, 2435, 2830
SspMI CTAG 6 cut(s) 509, 938, 1508, 1542, 1875, 2238
SstI GAGCTC 2 cut(s) 1360, 2011
StuI AGGCCT 1 cut(s) 2698
StyD4I CCNGG 3 cut(s) 363, 559, 2601
StyI CCWWGG 5 cut(s) 923, 1059, 1476, 2026, 2364
TaaI ACNGT 8 cut(s) 495, 1027, 1271, 1316, 1967, 2375, 2451, 2852
TaiI ACGT 3 cut(s) 101, 911, 1449
TaqI TCGA 3 cut(s) 525, 675, 903
TaqII GACCGA 2 cut(s) 857, 2130
TatI WGTACW 2 cut(s) 495, 1184
TauI GCSGC 2 cut(s) 840, 2884
TfiI GAWTC 6 cut(s) 603, 1451, 2018, 2190, 2606, 2768
TscAI CASTG 8 cut(s) 697, 1209, 1285, 1804, 1852, 2380, 2637, 2857
TseFI GTSAC 2 cut(s) 67, 2369
TseI GCWGC 5 cut(s) 696, 1712, 1715, 2740, 2837
Tsp45I GTSAC 2 cut(s) 67, 2369
TspGWI ACGGA 2 cut(s) 2808, 2853
TspRI CASTG 8 cut(s) 697, 1209, 1285, 1804, 1852, 2380, 2637, 2857
Van91I CCANNNNNTGG 1 cut(s) 880
Vha464I CTTAAG 1 cut(s) 2162
VpaK11BI GGWCC 5 cut(s) 314, 408, 530, 1030, 1610
VspI ATTAAT 1 cut(s) 956
XagI CCTNNNNNAGG 1 cut(s) 1475
XapI RAATTY 5 cut(s) 229, 279, 439, 1119, 1758
XceI RCATGY 3 cut(s) 199, 574, 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 958
XspI CTAG 6 cut(s) 509, 938, 1508, 1542, 1875, 2238
Zsp2I ATGCAT 1 cut(s) 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.