MD11G1206300.v1.1

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000487
Physical Location & Seq
Forward (+)
30097931 .. 30103121
5191 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1206300.v1.1.491

Sequence Viewer

Length: 2808 bp
ATGGATCGAACTCGAGCCACTGATCATGTGTTTCCCCTGCATGTGTTGATCCCTTTCTTTGTTATTGCAGTAGAGGCAAGCATTCAGGAATATGTCCCTATGAAGATTCTCATGGATGAGTGGAACATAAGACCAACCAGTTGGGTGGGTTCAGATCCTTGTGGTAATGGCAGCTGGGAAGGTGTTACGTGCACAAATTCGCATGTAACCTCCATAGCATTGGCAGATATGGCTTTGACAGGTTCGGTTCCCAGCGATATCGAGCTGTTATCTGAGCTTGAGGTTCTGAATCTGTCTTTTAACAGGGGCCTGAAAGGACTGCTTCCGGCATCAATTGGAAAGTTGACCAAACTAGTATACTTAAACCTGGTTGGTTGCAGTTTCTTCGGTCCAATTCCAGACACTATGGGATCTCTAAAGCAGCTTTCTTATCTATCTTTGAAAAACAATAGCTTCAGTGGCCGGATTCCACCTTCCATTGGTAATATGACCAATCTTTCCTTTCTGGACCTTTCTGACAACCAACTTCAGGGACCAATCCCAGTATCTAACAGGACTGCACCCGGTCTTGATATGTTACATAGGGCAAGGCACTTTCATTTTCAAAATAATCAGCTCTCTGGTACAATTCCTCCTCGACTTTTCAGTTCACAGATGGCTCTGTTACATGTGGTTTTTGACCGAAATAATCTAACTGGAGTCATACCTTCCACACTCGGACAAGTGCAGACACTGCAGGCAGTAATCCTTGATAGGAATTCCTTACATGGGTCCGTTCCCTCAAGCCTCAACAATCTAACAAAATTGACCGAACTGCACTTGTCCAACAATCAGCTGACTGGCACCATGCCAAACCTTACTAGCTTGAATGGCCTGAAGTATGTGGATATGAGCAATAATACCTTTGATGCAACAGATGTTCCTACTTGGTTTTTGACATTGCAGAATTTGACAACATTAATGATGGAGAACACGGGACTTCAGGGAGAAGTTCCTCCGGCCTTATTCAGCCTTTCCAGTTTGGAGACGGTGTTACTGAGGAACAACAAGATCAATGGTTTATTGGATCTTGAAACCATTTCTAGCAACCGTTTGCAGCTAATTGATCTGGAGAAGAACTTCATTACCGACCTTACACGAAGTGATGGAGGATCCAACTATACATTGATCCTCGTTGATAATCCAATTTGTGAGGGAACAAACATGGCAAATAACTACTGCACTGTTTCTCCAACCAGTTCCCCAATTTCGCCGCCACCAAGTAACTGTACGCAAGTTGCTTGTAGTCCAGGGCAGGTTTCTTCCCCCAACTGTAAATGTGCATATCCCTACACAGGAACTCTAGACTTCATATTTGTTTCTTTCTCCAACTTGGGAAACTTCATTTATTTCACAGCTCTTCAGGATCATCTCCTAAAAACCCTTCAGTCCTATAACCTTCCAGTGGATTCAGTTGCTCTTATTAATCCTTCCTGGGACTCCTCTTTTCACCTTAAACTCTACATACTAATCTTCCCGGCTGGCCAAGACCACTTCAACAAAACTGGATCTTCAGCCATTGCGTCCATGATTAGCAACCAGACTCTGGCGGGTGCGCGTCCAAGTTTTTATGGACCCTTCTCTTGTGTTTTCCCTTATGACAACCCGAATTCAGGAGGATCAAGCAAGGGCTTGATAATTGGTGCAGCAGTTGGTGGTTCCACACTTGTGTTATTGTTAGCCCTTCTAGGGTTTTATGCTCTCCACCAAAAGAGACAAGCAGATGATCCATGGAGAAGGTCAATCCTTTTGACCAGGACTTATAGCAGTGCTAATTCTCCGGACCTAAAAGGAGCACGGTTGTTCTCTTTTGAAGAGCTTAGGAAATACAGCAACGGTTTTTCAGACGCTAATGATGTTGGATCTGGTGGTTATGGAAAGGTGTATCGAGGAGTTCTTCCTACGGGCCAAATGGTTGCAATTAAAAGATCTAAAAGGGAATCTATGCAGGGAGGGCCTGAGTTTAAAGCTGAGGTTGAGCTTCTATCAAGAGTACATCATAAGAATCTTGTCAGCCTTGTTGGTTTTTGTTTGGAGCAAGGTGAACAAATCTTGGTATATGAGTATGTTCCGAATGGCGATTTATTTGGAATTCTTTCAGGGAAGTTTGGAATCAGGCTGAACTGGATGAGGAGACTTAAAGTAATTCTTGGTGCAGCAAGAGGTCTGGCTTATCTTCATGAACACGCAAACCCTCCCATTATACACCGCGACATTAAATCAAGCAACATATTGCTCGATAAAGATTTAACAGCAAAAGTTGCTGATTTCGGTCTCTCCAAGTCTATGTCTGACAGTGAAACAGATCACATAAGTACTCAAGTTAAGGGGACAATGGGCTACTTGGATCCCGAGTATTACATGACTCAACAGTTGACAGAGAAAAGTGACGTTTATAGCTTCGGCGTGGTAATGTTGGAGCTGATAACTGCTAGAAGGCCAATAGAGCAAGGGAAGTATATAGTGAGAGTGGTGCAGATGGCAATGGATAAGACAAAATCTCTGTACAACCTTCATCAAGTTCTTGACCCGGCTATGGGTTCGGGAACAGATCTGAAAGGTTTGGAAATTTTTGTTGATTTGGCGATGCTGTGCGTAGAAGAGCAGCAGGATAAACGCCCTAGAATGGGGGAAGTGGTGAAAGAGATCGAGAACATAATGCAGCTAGCTGGTTTGAACACTGGTGATCAATCAGCGTCCAGTTCACCAAGTTATGACTACACAAGTGCCGATGATTTGCTGCCGTCCTCAAGGCAGTCAAGTTCTTGA

Protein Analysis

936

Amino Acids

102.54

Weight (kDa)

6.01

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 81 - 172 2.3e-08 Leucine-rich repeat region
Pkinase PF00069 629 - 894 1.1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 631 - 895 1.4e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000487)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49760 AT5G49770
fragaria_vesca FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23870 FvH4_3g23881 FvH4_3g23881 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_3g23890 FvH4_5g27161 FvH4_5g27170
malus_domestica MD11G1206300.v1.1
prunus_persica Prupe.4G230700_v2.0.a1 Prupe.4G230700_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1 Prupe.4G231100_v2.0.a1
pyrus_communis pycom11g17930
rosa_chinensis RchiOBHm_Chr5g0042211 RchiOBHm_Chr5g0042261 RchiOBHm_Chr5g0042271 RchiOBHm_Chr5g0042311 RchiOBHm_Chr5g0042381 RchiOBHm_Chr7g0218261
rosa_laevigata RLG00000002384 RLG00000002385 RLG00000034112 RLG00000034114 RLG00000034116 RLG00000034118
rosa_multiflora Rmu_sc0001715.1_g000008 Rmu_sc0001715.1_g000012 Rmu_sc0001715.1_g000026 Rmu_sc0004956.1_g000008
rosa_roxburghii Rroxscaffold_1G00038360 Rroxscaffold_1G00038390 Rroxscaffold_1G00038400 Rroxscaffold_1G00038450 Rroxscaffold_3G00241180
rosa_rugosa Rorug05G0198800 Rorug05G0199000 Rorug05G0199100.1 Rorug05G0199200 Rorug05G0199300 Rorug07G0175900
rosa_samantha Rh5AG283400 Rh5AG283900 Rh5AG284100 Rh5AG284300 Rh5AG284900 Rh5AG285300 Rh5AG285700 Rh5BG289300 Rh5BG289900 Rh5BG290200 Rh5BG290400 Rh5CG320700 Rh5CG321200 Rh5CG321500 Rh5CG322100 Rh5CG322400 Rh5DG298600 Rh5DG299100 Rh5DG299400 Rh5DG300200 Rh7AG317500 Rh7BG308900 Rh7CG334400 Rh7DG316500
rosa_wichuraiana Rw0G001480 Rw0G001500 Rw0G001750 Rw0G001780 Rw0G015430 Rw5G026520 Rw7G026960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1285
AccB1I GGYRCC 1 cut(s) 842
AccB7I CCANNNNNTGG 1 cut(s) 1585
AccI GTMKAC 1 cut(s) 357
AccII CGCG 2 cut(s) 1597, 2250
AccIII TCCGGA 1 cut(s) 1819
AciI CCGC 3 cut(s) 1253, 1589, 2248
AcoI YGGCCR 2 cut(s) 460, 1522
AcsI RAATTY 6 cut(s) 196, 757, 946, 1648, 2130, 2607
AcuI CTGAAG 7 cut(s) 439, 512, 896, 965, 1385, 1409, 1536
AdeI CACNNNGTG 1 cut(s) 1142
AfaI GTAC 5 cut(s) 625, 1270, 2034, 2356, 2546
AflIII ACRYGT 1 cut(s) 667
AgsI TTSAA 7 cut(s) 442, 605, 868, 1073, 1537, 1853, 2716
AhlI ACTAGT 1 cut(s) 352
AjnI CCWGG 4 cut(s) 366, 1288, 1472, 1793
AleI CACNNNNGTG 1 cut(s) 1706
Alw21I GWGCWC 2 cut(s) 194, 1837
Alw26I GTCTC 4 cut(s) 1019, 1747, 2167, 2318
Alw44I GTGCAC 1 cut(s) 190
AlwNI CAGNNNCTG 2 cut(s) 733, 1585
Ama87I CYCGRG 2 cut(s) 12, 2390
Aor13HI TCCGGA 1 cut(s) 1819
AoxI GGCC 8 cut(s) 307, 460, 871, 999, 1522, 1945, 1994, 2477
ApaLI GTGCAC 1 cut(s) 190
ApeKI GCWGC 8 cut(s) 171, 421, 1096, 1685, 2195, 2644, 2701, 2779
ApoI RAATTY 6 cut(s) 196, 757, 946, 1648, 2130, 2607
ArsI GACNNNNNNTTYG 2 cut(s) 1337, 1369
AseI ATTAAT 2 cut(s) 959, 1464
Asp700I GAANNNNTTC 2 cut(s) 1118, 2134
AspLEI GCGC 1 cut(s) 1597
AspS9I GGNCC 9 cut(s) 307, 389, 508, 533, 771, 1613, 1822, 1945, 1994
AsuC2I CCSGG 3 cut(s) 564, 1517, 2570
AsuHPI GGTGA 5 cut(s) 1481, 2093, 2689, 2735, 2736
AsuNHI GCTAGC 1 cut(s) 2704
AvaI CYCGRG 2 cut(s) 12, 2390
AvaII GGWCC 6 cut(s) 389, 508, 533, 771, 1613, 1822
BaeGI GKGCMC 1 cut(s) 194
BalI TGGCCA 1 cut(s) 1524
BamHI GGATCC 2 cut(s) 1151, 2386
BanI GGYRCC 1 cut(s) 842
Bbv12I GWGCWC 2 cut(s) 194, 1837
BbvCI CCTCAGC 1 cut(s) 2010
BbvI GCAGC 8 cut(s) 183, 433, 1108, 1697, 2207, 2656, 2713, 2766
BccI CCATC 4 cut(s) 649, 958, 1139, 2512
BceAI ACGGC 1 cut(s) 2767
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BciT130I CCWGG 4 cut(s) 368, 1290, 1474, 1795
BclI TGATCA 2 cut(s) 22, 2725
BcnI CCSGG 3 cut(s) 564, 1517, 2570
BcoDI GTCTC 4 cut(s) 1019, 1747, 2167, 2318
BcuI ACTAGT 1 cut(s) 352
BfaI CTAG 8 cut(s) 353, 861, 1083, 1343, 1727, 2472, 2661, 2705
BfmI CTRYAG 1 cut(s) 734
BfuAI ACCTGC 1 cut(s) 1285
BglII AGATCT 2 cut(s) 1967, 2590
BisI GCNGC 9 cut(s) 172, 422, 1097, 1253, 1686, 2196, 2645, 2702, 2780
BlsI GCNGC 9 cut(s) 173, 423, 1098, 1254, 1687, 2197, 2646, 2703, 2781
BmcAI AGTACT 1 cut(s) 2356
Bme1390I CCNGG 7 cut(s) 368, 564, 1290, 1474, 1517, 1795, 2570
Bme18I GGWCC 6 cut(s) 389, 508, 533, 771, 1613, 1822
BmeT110I CYCGRG 2 cut(s) 12, 2390
BmgT120I GGNCC 9 cut(s) 307, 389, 508, 533, 771, 1613, 1822, 1945, 1994
BmiI GGNNCC 9 cut(s) 249, 308, 534, 772, 844, 1153, 1615, 1699, 2388
BmrFI CCNGG 7 cut(s) 368, 564, 1290, 1474, 1517, 1795, 2570
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 3 cut(s) 338, 898, 2616
BmtI GCTAGC 1 cut(s) 2708
BmuI ACTGGG 1 cut(s) 536
BpmI CTGGAG 2 cut(s) 717, 1130
Bpu10I CCTNAGC 2 cut(s) 1859, 2010
BpuEI CTTGAG 4 cut(s) 299, 766, 2343, 2773
BpuMI CCSGG 3 cut(s) 564, 1517, 2570
BsaAI YACGTR 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 2318
BsaJI CCNNGG 3 cut(s) 1289, 1473, 1769
BsaWI WCCGGW 1 cut(s) 1819
BsaXI ACNNNNNCTCC 6 cut(s) 616, 646, 1016, 1046, 1213, 1243
Bse3DI GCAATG 3 cut(s) 938, 1557, 2529
BseAI TCCGGA 1 cut(s) 1819
BseBI CCWGG 4 cut(s) 368, 1290, 1474, 1795
BseDI CCNNGG 3 cut(s) 1289, 1473, 1769
BseGI GGATG 2 cut(s) 121, 2172
BseMI GCAATG 3 cut(s) 938, 1557, 2529
BseMII CTCAG 4 cut(s) 264, 1028, 1989, 2001
BseRI GAGGAG 4 cut(s) 624, 1471, 1944, 2185
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 8 cut(s) 183, 433, 1108, 1697, 2207, 2656, 2713, 2766
BseYI CCCAGC 2 cut(s) 174, 251
BsgI GTGCAG 7 cut(s) 543, 746, 800, 1204, 1704, 2214, 2534
Bsh1236I CGCG 2 cut(s) 1597, 2250
BshFI GGCC 8 cut(s) 309, 462, 873, 1001, 1524, 1947, 1996, 2479
BshNI GGYRCC 1 cut(s) 842
BsiHKAI GWGCWC 2 cut(s) 194, 1837
BsiHKCI CYCGRG 2 cut(s) 12, 2390
BsiSI CCGG 7 cut(s) 326, 463, 564, 998, 1517, 1820, 2570
BslFI GGGAC 5 cut(s) 80, 546, 990, 1490, 2383
BsmAI GTCTC 4 cut(s) 1019, 1747, 2167, 2318
BsmBI CGTCTC 1 cut(s) 1019
BsmFI GGGAC 5 cut(s) 80, 546, 990, 1490, 2383
BsmI GAATGC 1 cut(s) 81
BsnI GGCC 8 cut(s) 309, 462, 873, 1001, 1524, 1947, 1996, 2479
Bso31I GGTCTC 1 cut(s) 2318
BsoBI CYCGRG 2 cut(s) 12, 2390
Bsp1286I GDGCHC 2 cut(s) 194, 1837
Bsp13I TCCGGA 1 cut(s) 1819
Bsp1407I TGTACA 1 cut(s) 2544
Bsp19I CCATGG 1 cut(s) 1769
BspACI CCGC 3 cut(s) 1253, 1589, 2248
BspANI GGCC 8 cut(s) 309, 462, 873, 1001, 1524, 1947, 1996, 2479
BspCNI CTCAG 4 cut(s) 265, 1029, 1990, 2002
BspEI TCCGGA 1 cut(s) 1819
BspFNI CGCG 2 cut(s) 1597, 2250
BspHI TCATGA 1 cut(s) 2218
BspLI GGNNCC 9 cut(s) 249, 308, 534, 772, 844, 1153, 1615, 1699, 2388
BspMAI CTGCAG 1 cut(s) 738
BspMI ACCTGC 1 cut(s) 1285
BspOI GCTAGC 1 cut(s) 2708
BspQI GCTCTTC 3 cut(s) 1404, 1848, 2634
BspT107I GGYRCC 1 cut(s) 842
BspTNI GGTCTC 1 cut(s) 2318
BsrDI GCAATG 3 cut(s) 938, 1557, 2529
BsrGI TGTACA 1 cut(s) 2544
BssECI CCNNGG 3 cut(s) 1289, 1473, 1769
BssNAI GTATAC 1 cut(s) 358
BssT1I CCWWGG 1 cut(s) 1769
Bst1107I GTATAC 1 cut(s) 358
Bst2UI CCWGG 4 cut(s) 368, 1290, 1474, 1795
Bst4CI ACNGT 9 cut(s) 1030, 1091, 1225, 1268, 1313, 1839, 1877, 2336, 2412
Bst6I CTCTTC 3 cut(s) 1404, 1848, 2634
BstAPI GCANNNNNTGC 2 cut(s) 733, 2300
BstAUI TGTACA 1 cut(s) 2544
BstBAI YACGTR 1 cut(s) 189
BstC8I GCNNGC 4 cut(s) 79, 738, 1522, 2706
BstDEI CTNAG 5 cut(s) 273, 1037, 1859, 1998, 2010
BstDSI CCRYGG 1 cut(s) 1769
BstF5I GGATG 2 cut(s) 121, 2172
BstFNI CGCG 2 cut(s) 1597, 2250
BstHHI GCGC 1 cut(s) 1597
BstMAI GTCTC 4 cut(s) 1019, 1747, 2167, 2318
BstMWI GCNNNNNNNGC 8 cut(s) 74, 230, 459, 733, 870, 1993, 2300, 2485
BstNI CCWGG 4 cut(s) 368, 1290, 1474, 1795
BstNSI RCATGY 3 cut(s) 44, 206, 671
BstSCI CCNGG 7 cut(s) 366, 562, 1288, 1472, 1515, 1793, 2568
BstSFI CTRYAG 1 cut(s) 734
BstSLI GKGCMC 1 cut(s) 194
BstUI CGCG 2 cut(s) 1597, 2250
BstV1I GCAGC 8 cut(s) 183, 433, 1108, 1697, 2207, 2656, 2713, 2766
BstX2I RGATCY 9 cut(s) 154, 410, 1066, 1151, 1547, 1901, 1967, 2386, 2590
BstXI CCANNNNNNTGG 3 cut(s) 141, 145, 220
BstYI RGATCY 9 cut(s) 154, 410, 1066, 1151, 1547, 1901, 1967, 2386, 2590
BstZ17I GTATAC 1 cut(s) 358
BsuRI GGCC 8 cut(s) 309, 462, 873, 1001, 1524, 1947, 1996, 2479
BtgI CCRYGG 1 cut(s) 1769
BtgZI GCGATG 1 cut(s) 2639
BtsCI GGATG 2 cut(s) 121, 2172
BtsI GCAGTG 2 cut(s) 731, 1813
BtsIMutI CAGTG 8 cut(s) 18, 463, 731, 1221, 1449, 1813, 2341, 2718
BveI ACCTGC 1 cut(s) 1285
Cac8I GCNNGC 4 cut(s) 79, 738, 1522, 2706
CaiI CAGNNNCTG 2 cut(s) 733, 1585
CciI TCATGA 1 cut(s) 2218
CfoI GCGC 1 cut(s) 1597
Cfr13I GGNCC 9 cut(s) 307, 389, 508, 533, 771, 1613, 1822, 1945, 1994
CseI GACGC 4 cut(s) 1551, 1586, 1895, 2724
CsiI ACCWGGT 1 cut(s) 366
Csp6I GTAC 5 cut(s) 624, 1269, 2033, 2355, 2545
CviQI GTAC 5 cut(s) 624, 1269, 2033, 2355, 2545
DdeI CTNAG 5 cut(s) 273, 1037, 1859, 1998, 2010
DraI TTTAAA 1 cut(s) 2005
DraIII CACNNNGTG 1 cut(s) 1142
EaeI YGGCCR 2 cut(s) 460, 1522
Eam1104I CTCTTC 3 cut(s) 1404, 1848, 2634
EarI CTCTTC 3 cut(s) 1404, 1848, 2634
Eco130I CCWWGG 1 cut(s) 1769
Eco31I GGTCTC 1 cut(s) 2318
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 6 cut(s) 389, 508, 533, 771, 1613, 1822
Eco57I CTGAAG 7 cut(s) 439, 512, 896, 965, 1385, 1409, 1536
Eco88I CYCGRG 2 cut(s) 12, 2390
EcoO109I RGGNCCY 2 cut(s) 307, 1994
EcoRI GAATTC 3 cut(s) 757, 1648, 2130
EcoRII CCWGG 4 cut(s) 366, 1288, 1472, 1793
EcoRV GATATC 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 1769
ErhI CCWWGG 1 cut(s) 1769
Esp3I CGTCTC 1 cut(s) 1019
FalI AAGNNNNNCTT 4 cut(s) 306, 338, 2172, 2204
FaqI GGGAC 5 cut(s) 80, 546, 990, 1490, 2383
FauI CCCGC 1 cut(s) 1582
FbaI TGATCA 2 cut(s) 22, 2725
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 9 cut(s) 172, 422, 1097, 1253, 1686, 2196, 2645, 2702, 2780
FokI GGATG 2 cut(s) 128, 2179
Fsp4HI GCNGC 9 cut(s) 172, 422, 1097, 1253, 1686, 2196, 2645, 2702, 2780
FspBI CTAG 8 cut(s) 353, 861, 1083, 1343, 1727, 2472, 2661, 2705
GlaI GCGC 1 cut(s) 1596
GluI GCNGC 9 cut(s) 172, 422, 1097, 1253, 1686, 2196, 2645, 2702, 2780
GsaI CCCAGC 2 cut(s) 178, 255
GsuI CTGGAG 2 cut(s) 717, 1130
HaeIII GGCC 8 cut(s) 309, 462, 873, 1001, 1524, 1947, 1996, 2479
HapII CCGG 7 cut(s) 326, 463, 564, 998, 1517, 1820, 2570
HgaI GACGC 4 cut(s) 1551, 1586, 1895, 2724
HhaI GCGC 1 cut(s) 1597
Hin6I GCGC 1 cut(s) 1595
HinP1I GCGC 1 cut(s) 1595
HincII GTYRAC 2 cut(s) 345, 2415
HindII GTYRAC 2 cut(s) 345, 2415
HpaII CCGG 7 cut(s) 326, 463, 564, 998, 1517, 1820, 2570
HphI GGTGA 5 cut(s) 1481, 2093, 2689, 2735, 2736
Hpy166II GTNNAC 7 cut(s) 192, 345, 358, 650, 2084, 2415, 2744
Hpy188I TCNGA 9 cut(s) 154, 274, 288, 517, 719, 1885, 2112, 2332, 2595
Hpy8I GTNNAC 7 cut(s) 192, 345, 358, 650, 2084, 2415, 2744
HpyCH4III ACNGT 9 cut(s) 1030, 1091, 1225, 1268, 1313, 1839, 1877, 2336, 2412
HpyCH4IV ACGT 2 cut(s) 188, 2430
HpyF10VI GCNNNNNNNGC 8 cut(s) 74, 230, 459, 733, 870, 1993, 2300, 2485
HpyF3I CTNAG 5 cut(s) 273, 1037, 1859, 1998, 2010
HpySE526I ACGT 2 cut(s) 188, 2430
HspAI GCGC 1 cut(s) 1595
Kpn2I TCCGGA 1 cut(s) 1819
Ksp22I TGATCA 2 cut(s) 22, 2725
LguI GCTCTTC 3 cut(s) 1404, 1848, 2634
LmnI GCTCC 3 cut(s) 1832, 2074, 2458
Lsp1109I GCAGC 8 cut(s) 183, 433, 1108, 1697, 2207, 2656, 2713, 2766
LweI GCATC 3 cut(s) 338, 898, 2616
MabI ACCWGGT 1 cut(s) 366
MaeI CTAG 8 cut(s) 353, 861, 1083, 1343, 1727, 2472, 2661, 2705
MaeII ACGT 2 cut(s) 188, 2430
MaeIII GTNAC 7 cut(s) 184, 205, 576, 663, 1032, 1262, 2426
MfeI CAATTG 1 cut(s) 333
MflI RGATCY 9 cut(s) 154, 410, 1066, 1151, 1547, 1901, 1967, 2386, 2590
MhlI GDGCHC 2 cut(s) 194, 1837
MlsI TGGCCA 1 cut(s) 1524
MluNI TGGCCA 1 cut(s) 1524
MlyI GAGTC 4 cut(s) 708, 1472, 1576, 2398
MmeI TCCRAC 6 cut(s) 849, 1179, 1256, 1392, 1879, 2436
Mox20I TGGCCA 1 cut(s) 1524
MroI TCCGGA 1 cut(s) 1819
MroXI GAANNNNTTC 2 cut(s) 1118, 2134
MscI TGGCCA 1 cut(s) 1524
MslI CAYNNNNRTG 1 cut(s) 1706
Msp20I TGGCCA 1 cut(s) 1524
MspA1I CMGCKG 2 cut(s) 174, 835
MspI CCGG 7 cut(s) 326, 463, 564, 998, 1517, 1820, 2570
MspR9I CCNGG 7 cut(s) 368, 564, 1290, 1474, 1517, 1795, 2570
MunI CAATTG 1 cut(s) 333
Mva1269I GAATGC 1 cut(s) 81
MvaI CCWGG 4 cut(s) 368, 1290, 1474, 1795
MvnI CGCG 2 cut(s) 1597, 2250
MwoI GCNNNNNNNGC 8 cut(s) 74, 230, 459, 733, 870, 1993, 2300, 2485
NciI CCSGG 3 cut(s) 564, 1517, 2570
NcoI CCATGG 1 cut(s) 1769
NheI GCTAGC 1 cut(s) 2704
NlaIV GGNNCC 9 cut(s) 249, 308, 534, 772, 844, 1153, 1615, 1699, 2388
NmuCI GTSAC 1 cut(s) 2426
NspI RCATGY 3 cut(s) 44, 206, 671
OliI CACNNNNGTG 1 cut(s) 1706
PaeR7I CTCGAG 1 cut(s) 12
PagI TCATGA 1 cut(s) 2218
PciI ACATGT 1 cut(s) 667
PciSI GCTCTTC 3 cut(s) 1404, 1848, 2634
PctI GAATGC 1 cut(s) 81
PdmI GAANNNNTTC 2 cut(s) 1118, 2134
PfeI GAWTC 7 cut(s) 106, 289, 466, 1448, 1979, 2044, 2151
PflMI CCANNNNNTGG 1 cut(s) 1585
PkrI GCNGC 9 cut(s) 173, 423, 1098, 1254, 1687, 2197, 2646, 2703, 2781
PleI GAGTC 4 cut(s) 707, 1472, 1576, 2398
PpsI GAGTC 4 cut(s) 707, 1472, 1576, 2398
Ppu21I YACGTR 1 cut(s) 189
PscI ACATGT 1 cut(s) 667
PshBI ATTAAT 2 cut(s) 959, 1464
Psp6I CCWGG 4 cut(s) 366, 1288, 1472, 1793
PspFI CCCAGC 2 cut(s) 174, 251
PspGI CCWGG 4 cut(s) 366, 1288, 1472, 1793
PspN4I GGNNCC 9 cut(s) 249, 308, 534, 772, 844, 1153, 1615, 1699, 2388
PspPI GGNCC 9 cut(s) 307, 389, 508, 533, 771, 1613, 1822, 1945, 1994
PspXI VCTCGAGB 1 cut(s) 12
PstI CTGCAG 1 cut(s) 738
PstNI CAGNNNCTG 2 cut(s) 733, 1585
PsuI RGATCY 9 cut(s) 154, 410, 1066, 1151, 1547, 1901, 1967, 2386, 2590
PvuII CAGCTG 2 cut(s) 174, 835
RsaI GTAC 5 cut(s) 625, 1270, 2034, 2356, 2546
RsaNI GTAC 5 cut(s) 624, 1269, 2033, 2355, 2545
RseI CAYNNNNRTG 1 cut(s) 1706
SapI GCTCTTC 3 cut(s) 1404, 1848, 2634
SatI GCNGC 9 cut(s) 172, 422, 1097, 1253, 1686, 2196, 2645, 2702, 2780
Sau96I GGNCC 9 cut(s) 307, 389, 508, 533, 771, 1613, 1822, 1945, 1994
ScaI AGTACT 1 cut(s) 2356
SchI GAGTC 4 cut(s) 708, 1472, 1576, 2398
ScrFI CCNGG 7 cut(s) 368, 564, 1290, 1474, 1517, 1795, 2570
SduI GDGCHC 2 cut(s) 194, 1837
SexAI ACCWGGT 1 cut(s) 366
SfaNI GCATC 3 cut(s) 338, 898, 2616
SfcI CTRYAG 1 cut(s) 734
Sfr274I CTCGAG 1 cut(s) 12
SinI GGWCC 6 cut(s) 389, 508, 533, 771, 1613, 1822
SlaI CTCGAG 1 cut(s) 12
SmiMI CAYNNNNRTG 1 cut(s) 1706
SmlI CTYRAG 5 cut(s) 12, 278, 781, 2358, 2788
SmoI CTYRAG 5 cut(s) 12, 278, 781, 2358, 2788
SpeI ACTAGT 1 cut(s) 352
SsiI CCGC 3 cut(s) 1253, 1589, 2248
SspMI CTAG 8 cut(s) 353, 861, 1083, 1343, 1727, 2472, 2661, 2705
StyD4I CCNGG 7 cut(s) 366, 562, 1288, 1472, 1515, 1793, 2568
StyI CCWWGG 1 cut(s) 1769
TaaI ACNGT 9 cut(s) 1030, 1091, 1225, 1268, 1313, 1839, 1877, 2336, 2412
TaiI ACGT 2 cut(s) 191, 2433
TaqI TCGA 7 cut(s) 7, 13, 261, 637, 1927, 2277, 2688
TaqII GACCGA 4 cut(s) 377, 696, 824, 2300
TatI WGTACW 3 cut(s) 2032, 2354, 2544
TauI GCSGC 1 cut(s) 1255
TfiI GAWTC 7 cut(s) 106, 289, 466, 1448, 1979, 2044, 2151
TscAI CASTG 8 cut(s) 25, 463, 738, 1228, 1449, 1813, 2341, 2725
TseFI GTSAC 1 cut(s) 2426
TseI GCWGC 8 cut(s) 171, 421, 1096, 1685, 2195, 2644, 2701, 2779
Tsp45I GTSAC 1 cut(s) 2426
TspDTI ATGAA 8 cut(s) 116, 587, 1111, 1339, 1372, 2207, 2235, 2543
TspGWI ACGGA 1 cut(s) 763
TspRI CASTG 8 cut(s) 25, 463, 738, 1228, 1449, 1813, 2341, 2725
Van91I CCANNNNNTGG 1 cut(s) 1585
VneI GTGCAC 1 cut(s) 190
VpaK11BI GGWCC 6 cut(s) 389, 508, 533, 771, 1613, 1822
VspI ATTAAT 2 cut(s) 959, 1464
XapI RAATTY 6 cut(s) 196, 757, 946, 1648, 2130, 2607
XbaI TCTAGA 1 cut(s) 1342
XceI RCATGY 3 cut(s) 44, 206, 671
XhoI CTCGAG 1 cut(s) 12
XmiI GTMKAC 1 cut(s) 357
XmnI GAANNNNTTC 2 cut(s) 1118, 2134
XspI CTAG 8 cut(s) 353, 861, 1083, 1343, 1727, 2472, 2661, 2705
ZrmI AGTACT 1 cut(s) 2356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.