FvH4_5g38490

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001161
Physical Location & Seq
Reverse (-)
28446769 .. 28450215
3447 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38490.t1

Sequence Viewer

Length: 717 bp
ATGTTCCCCCAGTATGTGAATGCCAAAGAGGATAATGTACAAACCAGTGGAGCAGATCAACAACATCTGCCAAGTTTAAGGACTCTCCTTAAAGTTTACAAGGATGCTATTTTTAATGGAGATGAACAGACTGTAGCAGAAATTGAGGCAAAGATAGAAATAGTAGAAAACAAGCAGAATGAATTAGTCCAGAAAGTATCATCTATGTCAGCAGAGGTAACATCAGGGAAGGAAAAGCTTATTCGTCTGCAAGCAGATTTTGATAATTGTAGAAAAAGATTTGAGAAAGAGAGACTTAACGTGAGGACTGATGCCCAAGGAGAAGTAATTGAGAGCCTTTTGCCCATGGTTGACAATTTTGAGAGAGCAAAACAACAAATTAAACCTGAAACTGAAAAGGAGAAGAAGATCGATACAAGTTACCAGGGAATATACAAGCAATTTGTTGAGATTATGAGGAGCTTGCGTGTGGCCTCTGTGCCAACTCTGGGAAAACCCTTTGATCCTTCGGTGCATGAAGCTATTGCACGAGAAGAGTCTCAAGAATTCCCGGATGGAATTGTCATTCAAGAAATTCGCCGTGGCTTTTTACTTGGAGGTCGACTTCTAAGACCAGCATTGGTTAAAGTCTCTATAGGACCTGGCAGTAAGAAATCTCCGGTGGCCACTGAGAAATCTTCAGGGTCACCTGCAACAACTGCCTCAGTAGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

239

Amino Acids

26.63

Weight (kDa)

6.25

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 52 - 211 1.3e-39 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 697
Acc36I ACCTGC 1 cut(s) 697
AccI GTMKAC 1 cut(s) 601
AclWI GGATC 1 cut(s) 497
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 2 cut(s) 545, 573
AcuI CTGAAG 1 cut(s) 663
AfaI GTAC 1 cut(s) 39
AfiI CCNNNNNNNGG 1 cut(s) 488
AgsI TTSAA 1 cut(s) 569
AjnI CCWGG 2 cut(s) 423, 640
AluBI AGCT 3 cut(s) 238, 462, 521
AluI AGCT 3 cut(s) 238, 462, 521
Alw26I GTCTC 3 cut(s) 286, 543, 634
AlwI GGATC 1 cut(s) 497
AoxI GGCC 2 cut(s) 471, 663
ApoI RAATTY 2 cut(s) 545, 573
AspS9I GGNCC 1 cut(s) 638
AsuC2I CCSGG 1 cut(s) 551
AsuHPI GGTGA 1 cut(s) 678
AvaII GGWCC 1 cut(s) 638
BalI TGGCCA 1 cut(s) 665
BauI CACGAG 1 cut(s) 528
BccI CCATC 1 cut(s) 548
BceAI ACGGC 1 cut(s) 564
BciT130I CCWGG 2 cut(s) 425, 642
BcnI CCSGG 1 cut(s) 551
BcoDI GTCTC 3 cut(s) 286, 543, 634
BfmI CTRYAG 2 cut(s) 132, 633
BfuAI ACCTGC 1 cut(s) 697
Bme1390I CCNGG 3 cut(s) 425, 551, 642
Bme18I GGWCC 1 cut(s) 638
BmgT120I GGNCC 1 cut(s) 638
BmrFI CCNGG 3 cut(s) 425, 551, 642
BmrI ACTGGG 1 cut(s) 4
BmsI GCATC 2 cut(s) 94, 301
BmuI ACTGGG 1 cut(s) 4
BpuEI CTTGAG 1 cut(s) 525
BpuMI CCSGG 1 cut(s) 551
Bsa29I ATCGAT 1 cut(s) 411
BsaJI CCNNGG 4 cut(s) 316, 345, 424, 580
BsaWI WCCGGW 1 cut(s) 658
BsaXI ACNNNNNCTCC 2 cut(s) 451, 481
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse1I ACTGG 2 cut(s) 10, 45
BseBI CCWGG 2 cut(s) 425, 642
BseCI ATCGAT 1 cut(s) 411
BseDI CCNNGG 4 cut(s) 316, 345, 424, 580
BseGI GGATG 2 cut(s) 109, 559
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMII CTCAG 2 cut(s) 660, 717
BseNI ACTGG 2 cut(s) 10, 45
BseRI GAGGAG 1 cut(s) 472
BshFI GGCC 2 cut(s) 473, 665
BshVI ATCGAT 1 cut(s) 411
BsiSI CCGG 2 cut(s) 551, 659
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 3 cut(s) 286, 543, 634
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 2 cut(s) 473, 665
Bsp1407I TGTACA 1 cut(s) 37
Bsp143I GATC 3 cut(s) 55, 408, 502
Bsp19I CCATGG 1 cut(s) 345
BspANI GGCC 2 cut(s) 473, 665
BspCNI CTCAG 2 cut(s) 661, 716
BspDI ATCGAT 1 cut(s) 411
BspMI ACCTGC 1 cut(s) 697
BspPI GGATC 1 cut(s) 497
BsrGI TGTACA 1 cut(s) 37
BsrI ACTGG 2 cut(s) 10, 45
BssECI CCNNGG 4 cut(s) 316, 345, 424, 580
BssMI GATC 3 cut(s) 55, 408, 502
BssSI CACGAG 1 cut(s) 528
BssT1I CCWWGG 2 cut(s) 316, 345
Bst2BI CACGAG 1 cut(s) 528
Bst2UI CCWGG 2 cut(s) 425, 642
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 1 cut(s) 528
BstAPI GCANNNNNTGC 1 cut(s) 698
BstAUI TGTACA 1 cut(s) 37
BstC8I GCNNGC 2 cut(s) 252, 464
BstDEI CTNAG 3 cut(s) 608, 669, 703
BstDSI CCRYGG 2 cut(s) 345, 580
BstEII GGTNACC 1 cut(s) 684
BstF5I GGATG 2 cut(s) 109, 559
BstKTI GATC 3 cut(s) 58, 411, 505
BstMAI GTCTC 3 cut(s) 286, 543, 634
BstMBI GATC 3 cut(s) 55, 408, 502
BstMWI GCNNNNNNNGC 1 cut(s) 698
BstNI CCWGG 2 cut(s) 425, 642
BstPI GGTNACC 1 cut(s) 684
BstSCI CCNGG 3 cut(s) 423, 549, 640
BstSFI CTRYAG 2 cut(s) 132, 633
Bsu15I ATCGAT 1 cut(s) 411
BsuRI GGCC 2 cut(s) 473, 665
BsuTUI ATCGAT 1 cut(s) 411
BtgI CCRYGG 2 cut(s) 345, 580
BtsCI GGATG 2 cut(s) 109, 559
BtsIMutI CAGTG 2 cut(s) 52, 666
BveI ACCTGC 1 cut(s) 697
Cac8I GCNNGC 2 cut(s) 252, 464
Cfr13I GGNCC 1 cut(s) 638
ClaI ATCGAT 1 cut(s) 411
Csp6I GTAC 1 cut(s) 38
CviAII CATG 2 cut(s) 346, 515
CviJI RGCY 7 cut(s) 238, 336, 462, 473, 521, 585, 665
CviKI_1 RGCY 7 cut(s) 238, 336, 462, 473, 521, 585, 665
CviQI GTAC 1 cut(s) 38
DdeI CTNAG 3 cut(s) 608, 669, 703
DpnI GATC 3 cut(s) 57, 410, 504
DpnII GATC 3 cut(s) 55, 408, 502
EaeI YGGCCR 1 cut(s) 663
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
Eco130I CCWWGG 2 cut(s) 316, 345
Eco47I GGWCC 1 cut(s) 638
Eco57I CTGAAG 1 cut(s) 663
Eco91I GGTNACC 1 cut(s) 684
EcoO109I RGGNCCY 1 cut(s) 638
EcoO65I GGTNACC 1 cut(s) 684
EcoRI GAATTC 1 cut(s) 545
EcoRII CCWGG 2 cut(s) 423, 640
EcoT14I CCWWGG 2 cut(s) 316, 345
ErhI CCWWGG 2 cut(s) 316, 345
FaeI CATG 2 cut(s) 349, 518
FaiI YATR 7 cut(s) 15, 206, 347, 433, 455, 516, 635
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FatI CATG 2 cut(s) 345, 514
FblI GTMKAC 1 cut(s) 601
FokI GGATG 2 cut(s) 116, 566
HaeIII GGCC 2 cut(s) 473, 665
HapII CCGG 2 cut(s) 551, 659
Hin1II CATG 2 cut(s) 349, 518
HincII GTYRAC 2 cut(s) 352, 602
HindII GTYRAC 2 cut(s) 352, 602
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 2 cut(s) 82, 536
HpaII CCGG 2 cut(s) 551, 659
HphI GGTGA 1 cut(s) 678
Hpy166II GTNNAC 3 cut(s) 97, 352, 602
Hpy188III TCNNGA 3 cut(s) 190, 542, 569
Hpy8I GTNNAC 3 cut(s) 97, 352, 602
HpyAV CCTTC 2 cut(s) 223, 516
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 4 cut(s) 250, 514, 527, 692
HpyF10VI GCNNNNNNNGC 1 cut(s) 698
HpyF3I CTNAG 3 cut(s) 608, 669, 703
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 2 cut(s) 349, 518
Kzo9I GATC 3 cut(s) 55, 408, 502
LmnI GCTCC 2 cut(s) 50, 459
LweI GCATC 2 cut(s) 94, 301
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 3 cut(s) 217, 419, 684
MalI GATC 3 cut(s) 57, 410, 504
MboI GATC 3 cut(s) 55, 408, 502
MboII GAAGA 4 cut(s) 415, 418, 545, 669
MlsI TGGCCA 1 cut(s) 665
MluNI TGGCCA 1 cut(s) 665
MlyI GAGTC 2 cut(s) 76, 545
MnlI CCTC 8 cut(s) 22, 139, 208, 297, 450, 484, 590, 712
Mox20I TGGCCA 1 cut(s) 665
MscI TGGCCA 1 cut(s) 665
MseI TTAA 6 cut(s) 77, 90, 114, 297, 381, 624
Msp20I TGGCCA 1 cut(s) 665
MspI CCGG 2 cut(s) 551, 659
MspR9I CCNGG 3 cut(s) 425, 551, 642
Mva1269I GAATGC 1 cut(s) 25
MvaI CCWGG 2 cut(s) 425, 642
MwoI GCNNNNNNNGC 1 cut(s) 698
NciI CCSGG 1 cut(s) 551
NcoI CCATGG 1 cut(s) 345
NdeII GATC 3 cut(s) 55, 408, 502
NlaIII CATG 2 cut(s) 349, 518
NmuCI GTSAC 1 cut(s) 684
PaqCI CACCTGC 1 cut(s) 697
PctI GAATGC 1 cut(s) 25
PfoI TCCNGGA 1 cut(s) 549
PleI GAGTC 2 cut(s) 76, 544
PpsI GAGTC 2 cut(s) 76, 544
PpuMI RGGWCCY 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 2 cut(s) 423, 640
PspEI GGTNACC 1 cut(s) 684
PspGI CCWGG 2 cut(s) 423, 640
PspPI GGNCC 1 cut(s) 638
PspPPI RGGWCCY 1 cut(s) 638
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
SalI GTCGAC 1 cut(s) 600
SaqAI TTAA 6 cut(s) 77, 90, 114, 297, 381, 624
Sau3AI GATC 3 cut(s) 55, 408, 502
Sau96I GGNCC 1 cut(s) 638
SchI GAGTC 2 cut(s) 76, 545
ScrFI CCNGG 3 cut(s) 425, 551, 642
SetI ASST 9 cut(s) 219, 240, 303, 388, 464, 523, 601, 643, 691
SfaNI GCATC 2 cut(s) 94, 301
SfcI CTRYAG 2 cut(s) 132, 633
SinI GGWCC 1 cut(s) 638
SmlI CTYRAG 1 cut(s) 540
SmoI CTYRAG 1 cut(s) 540
StyD4I CCNGG 3 cut(s) 423, 549, 640
StyI CCWWGG 2 cut(s) 316, 345
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 1 cut(s) 303
TaqI TCGA 2 cut(s) 411, 601
TatI WGTACW 1 cut(s) 37
Tru1I TTAA 6 cut(s) 77, 90, 114, 297, 381, 624
Tru9I TTAA 6 cut(s) 77, 90, 114, 297, 381, 624
TscAI CASTG 2 cut(s) 52, 673
TseFI GTSAC 1 cut(s) 684
Tsp45I GTSAC 1 cut(s) 684
TspDTI ATGAA 3 cut(s) 138, 195, 531
TspRI CASTG 2 cut(s) 52, 673
VpaK11BI GGWCC 1 cut(s) 638
XapI RAATTY 2 cut(s) 545, 573
XmiI GTMKAC 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.