Prupe.1G507100_v2.0.a1

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001161
Physical Location & Seq
Forward (+)
41852599 .. 41856025
3427 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G507100.1

Sequence Viewer

Length: 960 bp
ATGGCGAATTCGTTGTTTCTCCCAAACCACAGTTTCATAGCTCCCCCTCGCCTCTCTGCTTCCTTGTCCTCAAAATCCTCCAAACCCCTACCTCTTCCCCGTCTAGGGTTTTCTCTCAACAACTCTTCCTCTATTATTAGTCAGAGCAGCAGCAGCAGCAGCAGAAGCGGAAGCATAAGCAGAAGCAGGCGTGCCTCTTCCTTCAAATCTCATCTAGCTCCCCGAGACTCCGTTCCCCCAACGAATGCCAAAGAGGATCGTACTCGGAGTAATGTAGAAGCTTCGGAAAGTGGAGCAGATCAACACCATTTGCCCCGTTTGGGGACCCTCCTTCAAGTTTACAAGGAAGCTATATTTAACGGAGATGAAGAAACTGTATCAGAAGTTGAGGCCAAGATAGAAATACTTGAAAATGAGAAGAATAAATTAGTCAAGAAAGTATCTTCCTCGTCGGCAGAGATAACATCTGGGAAGGAGAAATTTATCCGCTTGCAAGCAGATTTTGATAATTGTAGAAAAAGATTTGAGAAGGAGAGACTTACGGTAAGGACGGATGCCCAAGGAGAAGTTATTGAGAGTCTTTTGCCCATGGTTGACAATTTTGAGAGAGCCAAACAATACATTAAACCCGAGACTGACAAGGAAAAAAAGGTTGATGCAAGTTACCAAGGTATATACAAGCAATTGGTGGAGACTATGAAGAGCTTGCATGTGGCTGTCGTGCCAACTGTGGGAAAGTCTTTTGATCCTTCGCTACATGAAGCTGTGGCACGTGAAGAGTCTCAAGAATTCAAGGAAGGGATTATAATTCAGGAAATCCGCCGTGGCTTTTTACTTGAAGGTCGGCTTCTAAGACCAGCAATGGTTAAAGTCTCAACAGGGCCTGGCAGTAAGAAAGCCCCTGTGGCCACCGAGAAATCATCTGGGTTGCCAGCAACAGCTGCCGGGGTGGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

320

Amino Acids

35.07

Weight (kDa)

9.38

Isoelectric Point (pI)

50.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 806
AciI CCGC 3 cut(s) 168, 487, 820
AclWI GGATC 2 cut(s) 264, 740
AcoI YGGCCR 1 cut(s) 906
AcsI RAATTY 3 cut(s) 7, 479, 788
AcvI CACGTG 1 cut(s) 773
AfaI GTAC 1 cut(s) 262
AfiI CCNNNNNNNGG 5 cut(s) 104, 105, 320, 321, 731
AgsI TTSAA 5 cut(s) 205, 335, 410, 793, 839
AjnI CCWGG 1 cut(s) 883
AluBI AGCT 7 cut(s) 41, 218, 281, 350, 705, 764, 941
AluI AGCT 7 cut(s) 41, 218, 281, 350, 705, 764, 941
Alw26I GTCTC 6 cut(s) 219, 529, 626, 686, 786, 877
AlwI GGATC 2 cut(s) 264, 740
AlwNI CAGNNNCTG 1 cut(s) 884
Ama87I CYCGRG 2 cut(s) 222, 629
AoxI GGCC 3 cut(s) 390, 881, 906
ApeKI GCWGC 6 cut(s) 147, 150, 153, 156, 159, 941
ApoI RAATTY 3 cut(s) 7, 479, 788
AspS9I GGNCC 2 cut(s) 324, 881
AsuC2I CCSGG 1 cut(s) 946
AvaI CYCGRG 2 cut(s) 222, 629
AvaII GGWCC 1 cut(s) 324
BalI TGGCCA 1 cut(s) 908
BbrPI CACGTG 1 cut(s) 773
BbvI GCAGC 6 cut(s) 159, 162, 165, 168, 171, 928
BceAI ACGGC 1 cut(s) 807
BciT130I CCWGG 1 cut(s) 885
BcnI CCSGG 1 cut(s) 946
BcoDI GTCTC 6 cut(s) 219, 529, 626, 686, 786, 877
BfaI CTAG 2 cut(s) 104, 215
BglI GCCNNNNNGGC 1 cut(s) 905
BisI GCNGC 6 cut(s) 148, 151, 154, 157, 160, 942
BlsI GCNGC 6 cut(s) 149, 152, 155, 158, 161, 943
Bme1390I CCNGG 2 cut(s) 885, 946
Bme18I GGWCC 1 cut(s) 324
BmeT110I CYCGRG 2 cut(s) 222, 629
BmgT120I GGNCC 2 cut(s) 324, 881
BmiI GGNNCC 2 cut(s) 325, 326
BmrFI CCNGG 2 cut(s) 885, 946
BmsI GCATC 2 cut(s) 544, 646
BpuEI CTTGAG 1 cut(s) 768
BpuMI CCSGG 1 cut(s) 946
BsaAI YACGTR 1 cut(s) 773
BsaJI CCNNGG 5 cut(s) 559, 588, 667, 823, 945
Bsc4I CCNNNNNNNGG 5 cut(s) 104, 105, 320, 321, 731
Bse3DI GCAATG 1 cut(s) 867
BseBI CCWGG 1 cut(s) 885
BseDI CCNNGG 5 cut(s) 559, 588, 667, 823, 945
BseGI GGATG 1 cut(s) 559
BseLI CCNNNNNNNGG 5 cut(s) 104, 105, 320, 321, 731
BseMI GCAATG 1 cut(s) 867
BseXI GCAGC 6 cut(s) 159, 162, 165, 168, 171, 928
BshFI GGCC 3 cut(s) 392, 883, 908
BsiHKCI CYCGRG 2 cut(s) 222, 629
BsiSI CCGG 1 cut(s) 945
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 5 cut(s) 104, 105, 320, 321, 731
BsmAI GTCTC 6 cut(s) 219, 529, 626, 686, 786, 877
BsmFI GGGAC 1 cut(s) 337
BsmI GAATGC 1 cut(s) 250
BsnI GGCC 3 cut(s) 392, 883, 908
BsoBI CYCGRG 2 cut(s) 222, 629
Bsp143I GATC 3 cut(s) 256, 298, 745
Bsp19I CCATGG 1 cut(s) 588
BspACI CCGC 3 cut(s) 168, 487, 820
BspANI GGCC 3 cut(s) 392, 883, 908
BspLI GGNNCC 2 cut(s) 325, 326
BspPI GGATC 2 cut(s) 264, 740
BspQI GCTCTTC 1 cut(s) 695
BsrDI GCAATG 1 cut(s) 867
BssECI CCNNGG 5 cut(s) 559, 588, 667, 823, 945
BssMI GATC 3 cut(s) 256, 298, 745
BssT1I CCWWGG 3 cut(s) 559, 588, 667
Bst2UI CCWGG 1 cut(s) 885
Bst4CI ACNGT 4 cut(s) 32, 376, 544, 730
Bst6I CTCTTC 5 cut(s) 99, 130, 202, 695, 771
BstAPI GCANNNNNTGC 1 cut(s) 941
BstBAI YACGTR 1 cut(s) 773
BstC8I GCNNGC 6 cut(s) 188, 192, 491, 495, 707, 933
BstDEI CTNAG 1 cut(s) 851
BstDSI CCRYGG 2 cut(s) 588, 823
BstF5I GGATG 1 cut(s) 559
BstKTI GATC 3 cut(s) 259, 301, 748
BstMAI GTCTC 6 cut(s) 219, 529, 626, 686, 786, 877
BstMBI GATC 3 cut(s) 256, 298, 745
BstMWI GCNNNNNNNGC 6 cut(s) 153, 156, 159, 165, 905, 941
BstNI CCWGG 1 cut(s) 885
BstNSI RCATGY 1 cut(s) 713
BstSCI CCNGG 2 cut(s) 883, 944
BstV1I GCAGC 6 cut(s) 159, 162, 165, 168, 171, 928
BsuRI GGCC 3 cut(s) 392, 883, 908
BtgI CCRYGG 2 cut(s) 588, 823
BtsCI GGATG 1 cut(s) 559
Cac8I GCNNGC 6 cut(s) 188, 192, 491, 495, 707, 933
CaiI CAGNNNCTG 1 cut(s) 884
Cfr13I GGNCC 2 cut(s) 324, 881
Csp6I GTAC 1 cut(s) 261
CviAII CATG 3 cut(s) 589, 710, 758
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 1 cut(s) 851
DpnI GATC 3 cut(s) 258, 300, 747
DpnII GATC 3 cut(s) 256, 298, 745
EaeI YGGCCR 1 cut(s) 906
Eam1104I CTCTTC 5 cut(s) 99, 130, 202, 695, 771
EarI CTCTTC 5 cut(s) 99, 130, 202, 695, 771
EciI GGCGGA 1 cut(s) 809
Eco130I CCWWGG 3 cut(s) 559, 588, 667
Eco47I GGWCC 1 cut(s) 324
Eco72I CACGTG 1 cut(s) 773
Eco88I CYCGRG 2 cut(s) 222, 629
EcoO109I RGGNCCY 2 cut(s) 324, 881
EcoRI GAATTC 2 cut(s) 7, 788
EcoRII CCWGG 1 cut(s) 883
EcoT14I CCWWGG 3 cut(s) 559, 588, 667
ErhI CCWWGG 3 cut(s) 559, 588, 667
FaeI CATG 3 cut(s) 592, 713, 761
FalI AAGNNNNNCTT 2 cut(s) 831, 863
FaqI GGGAC 1 cut(s) 337
FatI CATG 3 cut(s) 588, 709, 757
Fnu4HI GCNGC 6 cut(s) 148, 151, 154, 157, 160, 942
FokI GGATG 1 cut(s) 566
Fsp4HI GCNGC 6 cut(s) 148, 151, 154, 157, 160, 942
FspBI CTAG 2 cut(s) 104, 215
GluI GCNGC 6 cut(s) 148, 151, 154, 157, 160, 942
HaeIII GGCC 3 cut(s) 392, 883, 908
HapII CCGG 1 cut(s) 945
Hin1II CATG 3 cut(s) 592, 713, 761
HincII GTYRAC 1 cut(s) 595
HindII GTYRAC 1 cut(s) 595
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 3 cut(s) 227, 577, 779
HpaII CCGG 1 cut(s) 945
Hpy166II GTNNAC 2 cut(s) 340, 595
Hpy188I TCNGA 4 cut(s) 144, 267, 286, 382
Hpy188III TCNNGA 3 cut(s) 433, 785, 812
Hpy8I GTNNAC 2 cut(s) 340, 595
Hpy99I CGWCG 1 cut(s) 454
HpyAV CCTTC 7 cut(s) 211, 341, 466, 523, 759, 791, 833
HpyCH4III ACNGT 4 cut(s) 32, 376, 544, 730
HpyCH4IV ACGT 1 cut(s) 772
HpyCH4V TGCA 3 cut(s) 493, 659, 709
HpyF10VI GCNNNNNNNGC 6 cut(s) 153, 156, 159, 165, 905, 941
HpyF3I CTNAG 1 cut(s) 851
HpySE526I ACGT 1 cut(s) 772
Hsp92II CATG 3 cut(s) 592, 713, 761
KflI GGGWCCC 1 cut(s) 324
Kzo9I GATC 3 cut(s) 256, 298, 745
LguI GCTCTTC 1 cut(s) 695
LmnI GCTCC 3 cut(s) 46, 223, 293
Lsp1109I GCAGC 6 cut(s) 159, 162, 165, 168, 171, 928
LweI GCATC 2 cut(s) 544, 646
MaeI CTAG 2 cut(s) 104, 215
MaeII ACGT 1 cut(s) 772
MaeIII GTNAC 1 cut(s) 662
MalI GATC 3 cut(s) 258, 300, 747
MboI GATC 3 cut(s) 256, 298, 745
MboII GAAGA 8 cut(s) 86, 117, 189, 380, 430, 435, 712, 788
MfeI CAATTG 1 cut(s) 683
MlsI TGGCCA 1 cut(s) 908
MluCI AATT 8 cut(s) 7, 425, 479, 508, 598, 683, 788, 807
MluNI TGGCCA 1 cut(s) 908
MlyI GAGTC 3 cut(s) 221, 586, 788
Mox20I TGGCCA 1 cut(s) 908
MscI TGGCCA 1 cut(s) 908
MseI TTAA 3 cut(s) 357, 624, 867
Msp20I TGGCCA 1 cut(s) 908
MspA1I CMGCKG 1 cut(s) 941
MspI CCGG 1 cut(s) 945
MspR9I CCNGG 2 cut(s) 885, 946
MunI CAATTG 1 cut(s) 683
Mva1269I GAATGC 1 cut(s) 250
MvaI CCWGG 1 cut(s) 885
MwoI GCNNNNNNNGC 6 cut(s) 153, 156, 159, 165, 905, 941
NciI CCSGG 1 cut(s) 946
NcoI CCATGG 1 cut(s) 588
NdeII GATC 3 cut(s) 256, 298, 745
NlaIII CATG 3 cut(s) 592, 713, 761
NlaIV GGNNCC 2 cut(s) 325, 326
NspI RCATGY 1 cut(s) 713
PciSI GCTCTTC 1 cut(s) 695
PctI GAATGC 1 cut(s) 250
PkrI GCNGC 6 cut(s) 149, 152, 155, 158, 161, 943
PleI GAGTC 3 cut(s) 221, 585, 787
PmaCI CACGTG 1 cut(s) 773
PmlI CACGTG 1 cut(s) 773
PpsI GAGTC 3 cut(s) 221, 585, 787
Ppu21I YACGTR 1 cut(s) 773
PpuMI RGGWCCY 1 cut(s) 324
PsiI TTATAA 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 883
PspCI CACGTG 1 cut(s) 773
PspGI CCWGG 1 cut(s) 883
PspN4I GGNNCC 2 cut(s) 325, 326
PspPI GGNCC 2 cut(s) 324, 881
PspPPI RGGWCCY 1 cut(s) 324
PstNI CAGNNNCTG 1 cut(s) 884
PvuII CAGCTG 1 cut(s) 941
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 695
SaqAI TTAA 3 cut(s) 357, 624, 867
SatI GCNGC 6 cut(s) 148, 151, 154, 157, 160, 942
Sau3AI GATC 3 cut(s) 256, 298, 745
Sau96I GGNCC 2 cut(s) 324, 881
SchI GAGTC 3 cut(s) 221, 586, 788
ScrFI CCNGG 2 cut(s) 885, 946
SfaNI GCATC 2 cut(s) 544, 646
SinI GGWCC 1 cut(s) 324
SmlI CTYRAG 1 cut(s) 783
SmoI CTYRAG 1 cut(s) 783
Sse9I AATT 8 cut(s) 7, 425, 479, 508, 598, 683, 788, 807
SsiI CCGC 3 cut(s) 168, 487, 820
SspMI CTAG 2 cut(s) 104, 215
StyD4I CCNGG 2 cut(s) 883, 944
StyI CCWWGG 3 cut(s) 559, 588, 667
TaaI ACNGT 4 cut(s) 32, 376, 544, 730
TaiI ACGT 1 cut(s) 775
TasI AATT 8 cut(s) 7, 425, 479, 508, 598, 683, 788, 807
Tru1I TTAA 3 cut(s) 357, 624, 867
Tru9I TTAA 3 cut(s) 357, 624, 867
TseI GCWGC 6 cut(s) 147, 150, 153, 156, 159, 941
TspDTI ATGAA 4 cut(s) 25, 381, 713, 774
TspGWI ACGGA 3 cut(s) 220, 375, 566
VpaK11BI GGWCC 1 cut(s) 324
XapI RAATTY 3 cut(s) 7, 479, 788
XceI RCATGY 1 cut(s) 713
XspI CTAG 2 cut(s) 104, 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.