Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfiI | CCNNNNNNNGG | 1 cut(s) | 105 |
| AgsI | TTSAA | 1 cut(s) | 142 |
| AluBI | AGCT | 2 cut(s) | 17, 264 |
| AluI | AGCT | 2 cut(s) | 17, 264 |
| AspS9I | GGNCC | 1 cut(s) | 108 |
| AsuHPI | GGTGA | 1 cut(s) | 55 |
| AvaII | GGWCC | 1 cut(s) | 108 |
| BfaI | CTAG | 1 cut(s) | 72 |
| Bme18I | GGWCC | 1 cut(s) | 108 |
| BmgT120I | GGNCC | 1 cut(s) | 108 |
| BmiI | GGNNCC | 1 cut(s) | 110 |
| BmrI | ACTGGG | 1 cut(s) | 93 |
| BmsI | GCATC | 1 cut(s) | 121 |
| BmuI | ACTGGG | 1 cut(s) | 93 |
| BsaJI | CCNNGG | 1 cut(s) | 234 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 105 |
| Bse1I | ACTGG | 1 cut(s) | 99 |
| BseDI | CCNNGG | 1 cut(s) | 234 |
| BseGI | GGATG | 1 cut(s) | 136 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 105 |
| BseMII | CTCAG | 1 cut(s) | 156 |
| BseNI | ACTGG | 1 cut(s) | 99 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 105 |
| BsmI | GAATGC | 1 cut(s) | 52 |
| Bsp143I | GATC | 1 cut(s) | 82 |
| BspCNI | CTCAG | 1 cut(s) | 157 |
| BspLI | GGNNCC | 1 cut(s) | 110 |
| BsrI | ACTGG | 1 cut(s) | 99 |
| BssECI | CCNNGG | 1 cut(s) | 234 |
| BssMI | GATC | 1 cut(s) | 82 |
| Bst4CI | ACNGT | 1 cut(s) | 160 |
| BstC8I | GCNNGC | 1 cut(s) | 262 |
| BstDEI | CTNAG | 2 cut(s) | 25, 165 |
| BstF5I | GGATG | 1 cut(s) | 136 |
| BstKTI | GATC | 1 cut(s) | 85 |
| BstMBI | GATC | 1 cut(s) | 82 |
| BtsCI | GGATG | 1 cut(s) | 136 |
| Cac8I | GCNNGC | 1 cut(s) | 262 |
| Cfr13I | GGNCC | 1 cut(s) | 108 |
| CviAII | CATG | 1 cut(s) | 7 |
| CviJI | RGCY | 3 cut(s) | 17, 233, 264 |
| CviKI_1 | RGCY | 3 cut(s) | 17, 233, 264 |
| DdeI | CTNAG | 2 cut(s) | 25, 165 |
| DpnI | GATC | 1 cut(s) | 84 |
| DpnII | GATC | 1 cut(s) | 82 |
| Eco47I | GGWCC | 1 cut(s) | 108 |
| EcoO109I | RGGNCCY | 1 cut(s) | 108 |
| FaeI | CATG | 1 cut(s) | 10 |
| FaiI | YATR | 2 cut(s) | 8, 227 |
| FatI | CATG | 1 cut(s) | 6 |
| FokI | GGATG | 1 cut(s) | 143 |
| FspBI | CTAG | 1 cut(s) | 72 |
| Hin1II | CATG | 1 cut(s) | 10 |
| HinfI | GANTC | 1 cut(s) | 68 |
| HphI | GGTGA | 1 cut(s) | 55 |
| Hpy166II | GTNNAC | 1 cut(s) | 124 |
| Hpy188I | TCNGA | 1 cut(s) | 166 |
| Hpy188III | TCNNGA | 1 cut(s) | 211 |
| Hpy8I | GTNNAC | 1 cut(s) | 124 |
| HpyAV | CCTTC | 2 cut(s) | 122, 244 |
| HpyCH4III | ACNGT | 1 cut(s) | 160 |
| HpyCH4V | TGCA | 2 cut(s) | 50, 260 |
| HpyF3I | CTNAG | 2 cut(s) | 25, 165 |
| Hsp92II | CATG | 1 cut(s) | 10 |
| Kzo9I | GATC | 1 cut(s) | 82 |
| LmnI | GCTCC | 1 cut(s) | 77 |
| LpnPI | CCDG | 4 cut(s) | 26, 112, 224, 231 |
| LweI | GCATC | 1 cut(s) | 121 |
| MaeI | CTAG | 1 cut(s) | 72 |
| MaeIII | GTNAC | 1 cut(s) | 238 |
| MalI | GATC | 1 cut(s) | 84 |
| MboI | GATC | 1 cut(s) | 82 |
| MboII | GAAGA | 1 cut(s) | 158 |
| MluCI | AATT | 1 cut(s) | 203 |
| MnlI | CCTC | 4 cut(s) | 49, 99, 160, 229 |
| MseI | TTAA | 1 cut(s) | 117 |
| Mva1269I | GAATGC | 1 cut(s) | 52 |
| NdeII | GATC | 1 cut(s) | 82 |
| NlaIII | CATG | 1 cut(s) | 10 |
| NlaIV | GGNNCC | 1 cut(s) | 110 |
| NmeAIII | GCCGAG | 1 cut(s) | 259 |
| PctI | GAATGC | 1 cut(s) | 52 |
| PfeI | GAWTC | 1 cut(s) | 68 |
| PpuMI | RGGWCCY | 1 cut(s) | 108 |
| Psp5II | RGGWCCY | 1 cut(s) | 108 |
| PspN4I | GGNNCC | 1 cut(s) | 110 |
| PspPI | GGNCC | 1 cut(s) | 108 |
| PspPPI | RGGWCCY | 1 cut(s) | 108 |
| SaqAI | TTAA | 1 cut(s) | 117 |
| Sau3AI | GATC | 1 cut(s) | 82 |
| Sau96I | GGNCC | 1 cut(s) | 108 |
| SetI | ASST | 4 cut(s) | 19, 45, 240, 266 |
| SfaNI | GCATC | 1 cut(s) | 121 |
| SgeI | CNNG | 9 cut(s) | 19, 53, 84, 111, 139, 183, 223, 247, 258 |
| SinI | GGWCC | 1 cut(s) | 108 |
| Sse9I | AATT | 1 cut(s) | 203 |
| SspMI | CTAG | 1 cut(s) | 72 |
| TaaI | ACNGT | 1 cut(s) | 160 |
| TasI | AATT | 1 cut(s) | 203 |
| TfiI | GAWTC | 1 cut(s) | 68 |
| Tru1I | TTAA | 1 cut(s) | 117 |
| Tru9I | TTAA | 1 cut(s) | 117 |
| TspDTI | ATGAA | 3 cut(s) | 159, 165, 216 |
| VpaK11BI | GGWCC | 1 cut(s) | 108 |
| XspI | CTAG | 1 cut(s) | 72 |