MD00G1103800.v1.1

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0001161
Physical Location & Seq
Reverse (-)
21865548 .. 21868409
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1103800.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGCGAATTCGTTGTTTCTCCCAAATCACACTTTGATAGCTCCGCCTCCGCGTCGCCTCTCCGCCTCCGTCTCCTCGAAACCCAACAAACCCCAACCCCCTCTTCTTCCTTGTCCTCCTCCTCCCCTAGGGTTTATCAACTTCTCTCTCACCTCCAACTCATCCTCCTCCATTATTAATCCCAGCAGCAGAAGGCGAGCCGCCTCTTCCTTCAAGTCTCGTCTCGCTCCTCAGGACTCGGTTCCCCCAACGAATTCCAGAAAGGAAAGTAGTAAGAGCAATGCAGGACCTTCCGGAAGCGGAGGAGATGAAGAGCAGTTGCCCTCTTTGAAAACCCTCCTTAAAGTTTACAAGGAAGCTATTTTTAATGGAGATGGAAAAACTGTATCCGAAGTTGAGGCTATTATACAAGTGATAGAAAATGAGAAGAAAGAGTTAGTCGAGAAAGTATCTTCTGCATCAGCGGAGATAACGTCTGGGAAGGAGAAATTGATCCGCTTGCAAGCAGATTTTGATAATTGTAGAAAAAGATTTGACAAGGAGAGGCTGACCGTAAGGACGGATGCCCAAGGGGAAGTGATTGAGAGTCTTTTGCCCATGGTTGACAATTTTGAGAGAGCCAGGCAATACCTTAAACCCGAGACTGAACAGGAAAAAAAGATTGATGCAAGTTACCAAGGTATATACAAGCAATTTGTTGAGATTATGAGGAGCTTGGATGTGGCCGTCGTTCCAACCGTGGGAAAGCCTTTCGATCCTTCGTTGCATGAAGCTATTGCACGCGAAGAGTCTCAAGAGTTAAAGGAAGGGATTATAATCCAAGAAATCCGCCGTGGGTTTTTACTCGGAGGTCGGCTCCTGAGACCAGCTACAGTTAAAGTCTCATCAGGGCCCGGCAGTAAGAAATCCCCAGTGGCCACTGAGAAAACAACTGCAGGAGTGGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

317

Amino Acids

34.59

Weight (kDa)

9.34

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 140 - 295 9.1e-39 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 815
AccII CGCG 2 cut(s) 52, 783
AccIII TCCGGA 1 cut(s) 293
AciI CCGC 8 cut(s) 44, 50, 63, 201, 300, 464, 496, 829
AclWI GGATC 2 cut(s) 487, 749
AcoI YGGCCR 2 cut(s) 723, 915
AcsI RAATTY 2 cut(s) 7, 253
AfiI CCNNNNNNNGG 2 cut(s) 128, 740
AgsI TTSAA 2 cut(s) 214, 331
AjnI CCWGG 1 cut(s) 620
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AluBI AGCT 5 cut(s) 41, 359, 714, 773, 869
AluI AGCT 5 cut(s) 41, 359, 714, 773, 869
Alw26I GTCTC 7 cut(s) 76, 222, 227, 635, 795, 856, 886
AlwI GGATC 2 cut(s) 487, 749
Ama87I CYCGRG 1 cut(s) 638
Aor13HI TCCGGA 1 cut(s) 293
AoxI GGCC 3 cut(s) 723, 890, 915
ApaI GGGCCC 1 cut(s) 894
ApeKI GCWGC 1 cut(s) 186
ApoI RAATTY 2 cut(s) 7, 253
AseI ATTAAT 1 cut(s) 177
AspA2I CCTAGG 1 cut(s) 127
AspS9I GGNCC 3 cut(s) 287, 890, 891
AsuC2I CCSGG 1 cut(s) 894
AsuHPI GGTGA 1 cut(s) 142
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 1 cut(s) 287
AvrII CCTAGG 1 cut(s) 127
AxyI CCTNAGG 1 cut(s) 231
BaeGI GKGCMC 1 cut(s) 894
BalI TGGCCA 1 cut(s) 917
BanII GRGCYC 1 cut(s) 894
BbvI GCAGC 1 cut(s) 198
BccI CCATC 1 cut(s) 368
BceAI ACGGC 2 cut(s) 710, 816
BciT130I CCWGG 1 cut(s) 622
BciVI GTATCC 1 cut(s) 397
BcnI CCSGG 1 cut(s) 894
BcoDI GTCTC 7 cut(s) 76, 222, 227, 635, 795, 856, 886
BfaI CTAG 1 cut(s) 128
BfmI CTRYAG 2 cut(s) 870, 933
BfuI GTATCC 1 cut(s) 397
BisI GCNGC 2 cut(s) 187, 201
BlnI CCTAGG 1 cut(s) 127
BlsI GCNGC 2 cut(s) 188, 202
Bme1390I CCNGG 2 cut(s) 622, 894
Bme18I GGWCC 1 cut(s) 287
BmeT110I CYCGRG 1 cut(s) 638
BmgT120I GGNCC 3 cut(s) 287, 890, 891
BmiI GGNNCC 3 cut(s) 243, 857, 892
BmrFI CCNGG 2 cut(s) 622, 894
BmrI ACTGGG 1 cut(s) 905
BmsI GCATC 3 cut(s) 467, 553, 655
BmuI ACTGGG 1 cut(s) 905
BplI GAGNNNNNCTC 2 cut(s) 840, 872
BpuEI CTTGAG 1 cut(s) 777
BpuMI CCSGG 1 cut(s) 894
BsaBI GATNNNNATC 1 cut(s) 815
BsaI GGTCTC 1 cut(s) 856
BsaJI CCNNGG 6 cut(s) 127, 568, 597, 676, 738, 832
BsaWI WCCGGW 1 cut(s) 293
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 458, 488
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 740
Bse1I ACTGG 1 cut(s) 911
Bse21I CCTNAGG 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 286
Bse8I GATNNNNATC 1 cut(s) 815
BseAI TCCGGA 1 cut(s) 293
BseBI CCWGG 1 cut(s) 622
BseDI CCNNGG 6 cut(s) 127, 568, 597, 676, 738, 832
BseGI GGATG 3 cut(s) 161, 568, 724
BseJI GATNNNNATC 1 cut(s) 815
BseLI CCNNNNNNNGG 2 cut(s) 128, 740
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 3 cut(s) 245, 851, 912
BseNI ACTGG 1 cut(s) 911
BseRI GAGGAG 7 cut(s) 64, 108, 111, 157, 219, 318, 724
BseSI GKGCMC 1 cut(s) 894
BseXI GCAGC 1 cut(s) 198
BseYI CCCAGC 1 cut(s) 182
Bsh1236I CGCG 2 cut(s) 52, 783
BshFI GGCC 3 cut(s) 725, 892, 917
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 2 cut(s) 294, 894
BslI CCNNNNNNNGG 2 cut(s) 128, 740
BsmAI GTCTC 7 cut(s) 76, 222, 227, 635, 795, 856, 886
BsmBI CGTCTC 2 cut(s) 76, 227
BsnI GGCC 3 cut(s) 725, 892, 917
Bso31I GGTCTC 1 cut(s) 856
BsoBI CYCGRG 1 cut(s) 638
Bsp120I GGGCCC 1 cut(s) 890
Bsp1286I GDGCHC 1 cut(s) 894
Bsp13I TCCGGA 1 cut(s) 293
Bsp143I GATC 2 cut(s) 492, 754
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 8 cut(s) 44, 50, 63, 201, 300, 464, 496, 829
BspANI GGCC 3 cut(s) 725, 892, 917
BspCNI CTCAG 3 cut(s) 244, 852, 913
BspEI TCCGGA 1 cut(s) 293
BspFNI CGCG 2 cut(s) 52, 783
BspLI GGNNCC 3 cut(s) 243, 857, 892
BspMAI CTGCAG 1 cut(s) 937
BspPI GGATC 2 cut(s) 487, 749
BspQI GCTCTTC 1 cut(s) 306
BspTNI GGTCTC 1 cut(s) 856
BsrDI GCAATG 1 cut(s) 286
BsrI ACTGG 1 cut(s) 911
BssECI CCNNGG 6 cut(s) 127, 568, 597, 676, 738, 832
BssMI GATC 2 cut(s) 492, 754
BssT1I CCWWGG 4 cut(s) 127, 568, 597, 676
Bst2UI CCWGG 1 cut(s) 622
Bst4CI ACNGT 4 cut(s) 385, 553, 739, 874
Bst6I CTCTTC 4 cut(s) 108, 211, 306, 780
BstC8I GCNNGC 4 cut(s) 198, 500, 504, 781
BstDEI CTNAG 3 cut(s) 231, 860, 921
BstDSI CCRYGG 3 cut(s) 597, 738, 832
BstENI CCTNNNNNAGG 1 cut(s) 126
BstF5I GGATG 3 cut(s) 161, 568, 724
BstFNI CGCG 2 cut(s) 52, 783
BstKTI GATC 2 cut(s) 495, 757
BstMAI GTCTC 7 cut(s) 76, 222, 227, 635, 795, 856, 886
BstMBI GATC 2 cut(s) 492, 754
BstNI CCWGG 1 cut(s) 622
BstSCI CCNGG 2 cut(s) 620, 892
BstSFI CTRYAG 2 cut(s) 870, 933
BstSLI GKGCMC 1 cut(s) 894
BstUI CGCG 2 cut(s) 52, 783
BstV1I GCAGC 1 cut(s) 198
Bsu36I CCTNAGG 1 cut(s) 231
BsuI GTATCC 1 cut(s) 397
BsuRI GGCC 3 cut(s) 725, 892, 917
BtgI CCRYGG 3 cut(s) 597, 738, 832
BtsCI GGATG 3 cut(s) 161, 568, 724
BtsIMutI CAGTG 2 cut(s) 918, 918
Cac8I GCNNGC 4 cut(s) 198, 500, 504, 781
Cfr13I GGNCC 3 cut(s) 287, 890, 891
CseI GACGC 1 cut(s) 41
CviAII CATG 2 cut(s) 598, 767
DdeI CTNAG 3 cut(s) 231, 860, 921
DpnI GATC 2 cut(s) 494, 756
DpnII GATC 2 cut(s) 492, 754
EaeI YGGCCR 2 cut(s) 723, 915
Eam1104I CTCTTC 4 cut(s) 108, 211, 306, 780
EarI CTCTTC 4 cut(s) 108, 211, 306, 780
EciI GGCGGA 3 cut(s) 33, 52, 818
Eco130I CCWWGG 4 cut(s) 127, 568, 597, 676
Eco24I GRGCYC 1 cut(s) 894
Eco31I GGTCTC 1 cut(s) 856
Eco47I GGWCC 1 cut(s) 287
Eco81I CCTNAGG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 638
EcoNI CCTNNNNNAGG 1 cut(s) 126
EcoO109I RGGNCCY 2 cut(s) 287, 890
EcoRI GAATTC 2 cut(s) 7, 253
EcoRII CCWGG 1 cut(s) 620
EcoT14I CCWWGG 4 cut(s) 127, 568, 597, 676
EcoT38I GRGCYC 1 cut(s) 894
ErhI CCWWGG 4 cut(s) 127, 568, 597, 676
Esp3I CGTCTC 2 cut(s) 76, 227
FaeI CATG 2 cut(s) 601, 770
FaiI YATR 7 cut(s) 407, 599, 683, 685, 707, 768, 815
FatI CATG 2 cut(s) 597, 766
Fnu4HI GCNGC 2 cut(s) 187, 201
FokI GGATG 3 cut(s) 148, 575, 731
FriOI GRGCYC 1 cut(s) 894
Fsp4HI GCNGC 2 cut(s) 187, 201
FspBI CTAG 1 cut(s) 128
GluI GCNGC 2 cut(s) 187, 201
GsaI CCCAGC 1 cut(s) 186
HaeIII GGCC 3 cut(s) 725, 892, 917
HapII CCGG 2 cut(s) 294, 894
HgaI GACGC 1 cut(s) 41
Hin1II CATG 2 cut(s) 601, 770
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HinfI GANTC 3 cut(s) 236, 586, 788
HpaII CCGG 2 cut(s) 294, 894
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 2 cut(s) 349, 604
Hpy188I TCNGA 2 cut(s) 391, 848
Hpy188III TCNNGA 6 cut(s) 233, 258, 294, 442, 794, 859
Hpy8I GTNNAC 2 cut(s) 349, 604
Hpy99I CGWCG 2 cut(s) 57, 731
HpyAV CCTTC 6 cut(s) 186, 220, 300, 475, 768, 800
HpyCH4III ACNGT 4 cut(s) 385, 553, 739, 874
HpyCH4IV ACGT 1 cut(s) 473
HpyCH4V TGCA 7 cut(s) 284, 458, 502, 668, 766, 779, 935
HpyF3I CTNAG 3 cut(s) 231, 860, 921
HpySE526I ACGT 1 cut(s) 473
Hsp92II CATG 2 cut(s) 601, 770
Kpn2I TCCGGA 1 cut(s) 293
Kzo9I GATC 2 cut(s) 492, 754
LguI GCTCTTC 1 cut(s) 306
LmnI GCTCC 4 cut(s) 46, 232, 711, 861
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 3 cut(s) 467, 553, 655
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 473
MaeIII GTNAC 1 cut(s) 671
MalI GATC 2 cut(s) 494, 756
MboI GATC 2 cut(s) 492, 754
MboII GAAGA 7 cut(s) 95, 98, 198, 323, 439, 444, 797
MhlI GDGCHC 1 cut(s) 894
MlsI TGGCCA 1 cut(s) 917
MluCI AATT 6 cut(s) 7, 253, 488, 517, 607, 692
MluNI TGGCCA 1 cut(s) 917
MlyI GAGTC 3 cut(s) 230, 595, 797
MmeI TCCRAC 2 cut(s) 180, 758
Mox20I TGGCCA 1 cut(s) 917
MroI TCCGGA 1 cut(s) 293
MscI TGGCCA 1 cut(s) 917
MseI TTAA 6 cut(s) 177, 342, 366, 633, 800, 876
Msp20I TGGCCA 1 cut(s) 917
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 2 cut(s) 294, 894
MspR9I CCNGG 2 cut(s) 622, 894
MvaI CCWGG 1 cut(s) 622
MvnI CGCG 2 cut(s) 52, 783
NciI CCSGG 1 cut(s) 894
NcoI CCATGG 1 cut(s) 597
NdeII GATC 2 cut(s) 492, 754
NlaIII CATG 2 cut(s) 601, 770
NlaIV GGNNCC 3 cut(s) 243, 857, 892
PciSI GCTCTTC 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 735
PkrI GCNGC 2 cut(s) 188, 202
PleI GAGTC 3 cut(s) 230, 594, 796
PpsI GAGTC 3 cut(s) 230, 594, 796
PpuMI RGGWCCY 1 cut(s) 287
PshBI ATTAAT 1 cut(s) 177
PsiI TTATAA 1 cut(s) 815
Psp5II RGGWCCY 1 cut(s) 287
Psp6I CCWGG 1 cut(s) 620
PspFI CCCAGC 1 cut(s) 182
PspGI CCWGG 1 cut(s) 620
PspN4I GGNNCC 3 cut(s) 243, 857, 892
PspOMI GGGCCC 1 cut(s) 890
PspPI GGNCC 3 cut(s) 287, 890, 891
PspPPI RGGWCCY 1 cut(s) 287
PstI CTGCAG 1 cut(s) 937
SapI GCTCTTC 1 cut(s) 306
SaqAI TTAA 6 cut(s) 177, 342, 366, 633, 800, 876
SatI GCNGC 2 cut(s) 187, 201
Sau3AI GATC 2 cut(s) 492, 754
Sau96I GGNCC 3 cut(s) 287, 890, 891
SchI GAGTC 3 cut(s) 230, 595, 797
ScrFI CCNGG 2 cut(s) 622, 894
SduI GDGCHC 1 cut(s) 894
SfaNI GCATC 3 cut(s) 467, 553, 655
SfcI CTRYAG 2 cut(s) 870, 933
SinI GGWCC 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 792
SmoI CTYRAG 1 cut(s) 792
Sse9I AATT 6 cut(s) 7, 253, 488, 517, 607, 692
SsiI CCGC 8 cut(s) 44, 50, 63, 201, 300, 464, 496, 829
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 2 cut(s) 620, 892
StyI CCWWGG 4 cut(s) 127, 568, 597, 676
TaaI ACNGT 4 cut(s) 385, 553, 739, 874
TaiI ACGT 1 cut(s) 476
TaqI TCGA 3 cut(s) 77, 441, 753
TasI AATT 6 cut(s) 7, 253, 488, 517, 607, 692
TauI GCSGC 1 cut(s) 203
Tru1I TTAA 6 cut(s) 177, 342, 366, 633, 800, 876
Tru9I TTAA 6 cut(s) 177, 342, 366, 633, 800, 876
TscAI CASTG 2 cut(s) 918, 925
TseI GCWGC 1 cut(s) 186
TspDTI ATGAA 2 cut(s) 324, 783
TspGWI ACGGA 2 cut(s) 58, 575
TspRI CASTG 2 cut(s) 918, 925
VpaK11BI GGWCC 1 cut(s) 287
VspI ATTAAT 1 cut(s) 177
XagI CCTNNNNNAGG 1 cut(s) 126
XapI RAATTY 2 cut(s) 7, 253
XmaJI CCTAGG 1 cut(s) 127
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.