Rh7DG475200

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001161
Physical Location & Seq
Reverse (-)
67892670 .. 67896264
3595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG475200.1

Sequence Viewer

Length: 747 bp
ATGGCGAACTCGTTGTTTATCCCAAACAACTACTTCGTCATACCTCCTCCCGTTTCTGCTTCCTTCTCCTCAAAACCCATTGCTCCTCCTCTACCTCTTCTAGGCTTTTCAGTCACCAACTCTTCCCCCATAGTCTTACATAGCCGCAGCAGTAGCAGGCGTGCCTCGTTTTTCAAATCTCATCTTTCTGCTCGAGGCTCTGTTCCCCCAGTGAATGCCAAAGAGGATAATGTAGATTCTAGTGGAGCAGATCAACAACATTTGCCCAGTTTGAGGACCCTCCTTAAAGTTTACAAGGATGCTATTTTCAATGAAGATGAAAAGACTGTATCCGAAATTGAGGCAAGGATAGAAGTAATAGAAAATAAGCAGAATGAATTAGTCCAGAAAGTATCATCTATATCTGCAGAGGTAACATCAGGGAAGGAAAAGCTTATCCGCTTGCAAGCAGATTTTGATAATTGTAGAAAAAGATTTGAGAAAGAGAGACTAACTGTGAGGACTGATGCTCAAGGAGAAGTAATTGAGAGCCTTTTGCCCATGGTTGACAATTTTGAGAGAGCAAAACAACAGATTAGACCTGAAACTGAAAAGGAGAAGAAGATTGATACAAGTTACCAAGGTATATACAAGCAATTTGTTGAGATTATGAGGAGCTTGCGTGTGGCCTCTGTGCCAACTAAGGGAAAGCCTTTTGATCCTTCGGTAAGTGACTGCTTTTGGTTCAACTTATATTCTAGATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

248

Amino Acids

28.05

Weight (kDa)

8.95

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 120 - 236 3.2e-21 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 145, 439
AclWI GGATC 1 cut(s) 692
AfiI CCNNNNNNNGG 3 cut(s) 101, 273, 683
AgsI TTSAA 3 cut(s) 175, 310, 727
AluBI AGCT 2 cut(s) 433, 657
AluI AGCT 2 cut(s) 433, 657
Alw26I GTCTC 1 cut(s) 481
AlwI GGATC 1 cut(s) 692
Ama87I CYCGRG 1 cut(s) 192
AoxI GGCC 1 cut(s) 666
ApeKI GCWGC 1 cut(s) 147
AspS9I GGNCC 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 192
AvaII GGWCC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 159
BciVI GTATCC 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 481
BfaI CTAG 3 cut(s) 101, 240, 738
BfmI CTRYAG 1 cut(s) 405
BfuI GTATCC 1 cut(s) 340
BisI GCNGC 2 cut(s) 145, 148
BlsI GCNGC 2 cut(s) 146, 149
Bme18I GGWCC 1 cut(s) 276
BmeT110I CYCGRG 1 cut(s) 192
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 1 cut(s) 278
BmrI ACTGGG 2 cut(s) 203, 261
BmsI GCATC 2 cut(s) 289, 496
BmuI ACTGGG 2 cut(s) 203, 261
BpuEI CTTGAG 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 540, 619
BsaXI ACNNNNNCTCC 2 cut(s) 646, 676
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 273, 683
Bse1I ACTGG 2 cut(s) 209, 267
Bse3DI GCAATG 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 540, 619
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 3 cut(s) 101, 273, 683
BseMI GCAATG 1 cut(s) 78
BseNI ACTGG 2 cut(s) 209, 267
BseRI GAGGAG 5 cut(s) 36, 58, 75, 78, 667
BseXI GCAGC 1 cut(s) 159
BshFI GGCC 1 cut(s) 668
BsiHKCI CYCGRG 1 cut(s) 192
BslI CCNNNNNNNGG 3 cut(s) 101, 273, 683
BsmAI GTCTC 1 cut(s) 481
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 1 cut(s) 668
BsoBI CYCGRG 1 cut(s) 192
Bsp143I GATC 2 cut(s) 250, 697
Bsp19I CCATGG 1 cut(s) 540
BspACI CCGC 2 cut(s) 145, 439
BspANI GGCC 1 cut(s) 668
BspLI GGNNCC 1 cut(s) 278
BspMAI CTGCAG 1 cut(s) 409
BspPI GGATC 1 cut(s) 692
BsrDI GCAATG 1 cut(s) 78
BsrI ACTGG 2 cut(s) 209, 267
BssECI CCNNGG 2 cut(s) 540, 619
BssMI GATC 2 cut(s) 250, 697
BssT1I CCWWGG 2 cut(s) 540, 619
Bst4CI ACNGT 2 cut(s) 328, 496
Bst6I CTCTTC 2 cut(s) 102, 127
BstC8I GCNNGC 5 cut(s) 158, 162, 443, 447, 659
BstDEI CTNAG 1 cut(s) 681
BstDSI CCRYGG 1 cut(s) 540
BstENI CCTNNNNNAGG 1 cut(s) 99
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 2 cut(s) 253, 700
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 2 cut(s) 250, 697
BstMWI GCNNNNNNNGC 1 cut(s) 153
BstSFI CTRYAG 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 159
BsuI GTATCC 1 cut(s) 340
BsuRI GGCC 1 cut(s) 668
BtgI CCRYGG 1 cut(s) 540
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 5 cut(s) 158, 162, 443, 447, 659
Cfr13I GGNCC 1 cut(s) 276
CviAII CATG 1 cut(s) 541
CviJI RGCY 8 cut(s) 105, 144, 198, 433, 531, 657, 668, 691
CviKI_1 RGCY 8 cut(s) 105, 144, 198, 433, 531, 657, 668, 691
DdeI CTNAG 1 cut(s) 681
DpnI GATC 2 cut(s) 252, 699
DpnII GATC 2 cut(s) 250, 697
Eam1104I CTCTTC 2 cut(s) 102, 127
EarI CTCTTC 2 cut(s) 102, 127
Eco130I CCWWGG 2 cut(s) 540, 619
Eco47I GGWCC 1 cut(s) 276
Eco88I CYCGRG 1 cut(s) 192
EcoNI CCTNNNNNAGG 1 cut(s) 99
EcoO109I RGGNCCY 1 cut(s) 276
EcoT14I CCWWGG 2 cut(s) 540, 619
ErhI CCWWGG 2 cut(s) 540, 619
FaeI CATG 1 cut(s) 544
FaiI YATR 9 cut(s) 41, 131, 141, 401, 542, 626, 628, 650, 733
FatI CATG 1 cut(s) 540
Fnu4HI GCNGC 2 cut(s) 145, 148
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 2 cut(s) 145, 148
FspBI CTAG 3 cut(s) 101, 240, 738
GluI GCNGC 2 cut(s) 145, 148
HaeIII GGCC 1 cut(s) 668
Hin1II CATG 1 cut(s) 544
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 1 cut(s) 236
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 2 cut(s) 292, 547
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 2 cut(s) 385, 738
Hpy8I GTNNAC 2 cut(s) 292, 547
HpyAV CCTTC 3 cut(s) 73, 418, 711
HpyCH4III ACNGT 2 cut(s) 328, 496
HpyCH4V TGCA 2 cut(s) 407, 445
HpyF10VI GCNNNNNNNGC 1 cut(s) 153
HpyF3I CTNAG 1 cut(s) 681
Hsp92II CATG 1 cut(s) 544
Kzo9I GATC 2 cut(s) 250, 697
LmnI GCTCC 3 cut(s) 88, 245, 654
LpnPI CCDG 6 cut(s) 142, 222, 280, 398, 405, 594
Lsp1109I GCAGC 1 cut(s) 159
LweI GCATC 2 cut(s) 289, 496
MaeI CTAG 3 cut(s) 101, 240, 738
MaeIII GTNAC 4 cut(s) 112, 412, 614, 710
MalI GATC 2 cut(s) 252, 699
MboI GATC 2 cut(s) 250, 697
MboII GAAGA 5 cut(s) 89, 114, 326, 610, 613
MluCI AATT 6 cut(s) 336, 377, 460, 522, 550, 635
MseI TTAA 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 220
MwoI GCNNNNNNNGC 1 cut(s) 153
NcoI CCATGG 1 cut(s) 540
NdeII GATC 2 cut(s) 250, 697
NlaIII CATG 1 cut(s) 544
NlaIV GGNNCC 1 cut(s) 278
NmuCI GTSAC 2 cut(s) 112, 710
PaeR7I CTCGAG 1 cut(s) 192
PctI GAATGC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 2 cut(s) 146, 149
PpuMI RGGWCCY 1 cut(s) 276
Psp5II RGGWCCY 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 278
PspPI GGNCC 1 cut(s) 276
PspPPI RGGWCCY 1 cut(s) 276
PspXI VCTCGAGB 1 cut(s) 192
PstI CTGCAG 1 cut(s) 409
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 2 cut(s) 145, 148
Sau3AI GATC 2 cut(s) 250, 697
Sau96I GGNCC 1 cut(s) 276
SetI ASST 7 cut(s) 46, 97, 414, 435, 583, 625, 659
SfaNI GCATC 2 cut(s) 289, 496
SfcI CTRYAG 1 cut(s) 405
Sfr274I CTCGAG 1 cut(s) 192
SinI GGWCC 1 cut(s) 276
SlaI CTCGAG 1 cut(s) 192
SmlI CTYRAG 2 cut(s) 192, 510
SmoI CTYRAG 2 cut(s) 192, 510
Sse9I AATT 6 cut(s) 336, 377, 460, 522, 550, 635
SsiI CCGC 2 cut(s) 145, 439
SspMI CTAG 3 cut(s) 101, 240, 738
StyI CCWWGG 2 cut(s) 540, 619
TaaI ACNGT 2 cut(s) 328, 496
TaqI TCGA 1 cut(s) 193
TasI AATT 6 cut(s) 336, 377, 460, 522, 550, 635
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 216
TseFI GTSAC 2 cut(s) 112, 710
TseI GCWGC 1 cut(s) 147
Tsp45I GTSAC 2 cut(s) 112, 710
TspDTI ATGAA 3 cut(s) 327, 333, 390
TspRI CASTG 1 cut(s) 216
VpaK11BI GGWCC 1 cut(s) 276
XagI CCTNNNNNAGG 1 cut(s) 99
XbaI TCTAGA 1 cut(s) 737
XhoI CTCGAG 1 cut(s) 192
XspI CTAG 3 cut(s) 101, 240, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.