Rh7DG477400

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001161
Physical Location & Seq
Reverse (-)
68160429 .. 68161838
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG477400.1

Sequence Viewer

Length: 372 bp
ATGGTTGACAATTTTGAGAGAGCAAAATTACAAATTAGACCTGAAACTGAAAATGAGAAGAAAATTGATACAAGTTACCAGGGTATCTACAAGCAATTTGTTGAGATTATGAAGAGTTTGCATATGGCATCTGTGCCAACTATGGGAAAATCCTGTGATCCTTCGGTGCATGAAGCTAGTGCACTAGAAGAATCTCAAGAATTCCCAGAGGGGATTGTCATTCAAGAAATTAGCCATGGCTTTTTAGTTGGTGGTCGACTTCTTAGACCAGCAATGGTTAAAGTCTCTATAGGGCCTGGCAGTAAGAAGACCCCTGTGGCCACTGAGAAATCATCAGGGTCACCTGCAACAGCTGCTTTGGTAGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

123

Amino Acids

13.37

Weight (kDa)

5.92

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 2 - 96 1.3e-20 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 352
Acc36I ACCTGC 1 cut(s) 352
AccI GTMKAC 1 cut(s) 256
AclWI GGATC 1 cut(s) 152
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 1 cut(s) 200
AfiI CCNNNNNNNGG 1 cut(s) 143
AgsI TTSAA 1 cut(s) 224
AjnI CCWGG 2 cut(s) 78, 295
AluBI AGCT 2 cut(s) 176, 353
AluI AGCT 2 cut(s) 176, 353
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 1 cut(s) 289
Alw44I GTGCAC 1 cut(s) 180
AlwI GGATC 1 cut(s) 152
AoxI GGCC 2 cut(s) 293, 318
ApaLI GTGCAC 1 cut(s) 180
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 333
BaeGI GKGCMC 1 cut(s) 184
BaeI ACNNNNGTAYC 2 cut(s) 67, 100
BalI TGGCCA 1 cut(s) 320
BbsI GAAGAC 1 cut(s) 314
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 1 cut(s) 340
BciT130I CCWGG 2 cut(s) 80, 297
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 2 cut(s) 177, 185
BfmI CTRYAG 1 cut(s) 288
BfuAI ACCTGC 1 cut(s) 352
BisI GCNGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 355
Bme1390I CCNGG 2 cut(s) 80, 297
BmgT120I GGNCC 1 cut(s) 293
BmrFI CCNGG 2 cut(s) 80, 297
BmsI GCATC 1 cut(s) 137
BpiI GAAGAC 1 cut(s) 314
BpuEI CTTGAG 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 79, 235
Bsc4I CCNNNNNNNGG 1 cut(s) 143
Bse3DI GCAATG 1 cut(s) 279
BseBI CCWGG 2 cut(s) 80, 297
BseDI CCNNGG 2 cut(s) 79, 235
BseLI CCNNNNNNNGG 1 cut(s) 143
BseMI GCAATG 1 cut(s) 279
BseMII CTCAG 1 cut(s) 315
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 1 cut(s) 340
BshFI GGCC 2 cut(s) 295, 320
BsiHKAI GWGCWC 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 143
BsmAI GTCTC 1 cut(s) 289
BsnI GGCC 2 cut(s) 295, 320
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 1 cut(s) 157
Bsp19I CCATGG 1 cut(s) 235
BspANI GGCC 2 cut(s) 295, 320
BspCNI CTCAG 1 cut(s) 316
BspMI ACCTGC 1 cut(s) 352
BspPI GGATC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 279
BssECI CCNNGG 2 cut(s) 79, 235
BssMI GATC 1 cut(s) 157
BssT1I CCWWGG 1 cut(s) 235
Bst2UI CCWGG 2 cut(s) 80, 297
Bst6I CTCTTC 1 cut(s) 107
BstAPI GCANNNNNTGC 1 cut(s) 353
BstDEI CTNAG 2 cut(s) 263, 324
BstDSI CCRYGG 1 cut(s) 235
BstEII GGTNACC 1 cut(s) 339
BstKTI GATC 1 cut(s) 160
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 1 cut(s) 157
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstNI CCWGG 2 cut(s) 80, 297
BstPI GGTNACC 1 cut(s) 339
BstSCI CCNGG 2 cut(s) 78, 295
BstSFI CTRYAG 1 cut(s) 288
BstSLI GKGCMC 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 340
BstV2I GAAGAC 1 cut(s) 314
BsuRI GGCC 2 cut(s) 295, 320
BtgI CCRYGG 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 321
BveI ACCTGC 1 cut(s) 352
Cfr13I GGNCC 1 cut(s) 293
CviAII CATG 2 cut(s) 170, 236
CviJI RGCY 6 cut(s) 176, 234, 240, 295, 320, 353
CviKI_1 RGCY 6 cut(s) 176, 234, 240, 295, 320, 353
DdeI CTNAG 2 cut(s) 263, 324
DpnI GATC 1 cut(s) 159
DpnII GATC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
Eco130I CCWWGG 1 cut(s) 235
Eco91I GGTNACC 1 cut(s) 339
EcoO109I RGGNCCY 1 cut(s) 293
EcoO65I GGTNACC 1 cut(s) 339
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 2 cut(s) 78, 295
EcoT14I CCWWGG 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 2 cut(s) 173, 239
FaiI YATR 7 cut(s) 110, 123, 125, 143, 171, 237, 290
FatI CATG 2 cut(s) 169, 235
FauNDI CATATG 1 cut(s) 123
FblI GTMKAC 1 cut(s) 256
Fnu4HI GCNGC 1 cut(s) 354
Fsp4HI GCNGC 1 cut(s) 354
FspBI CTAG 2 cut(s) 177, 185
GluI GCNGC 1 cut(s) 354
HaeIII GGCC 2 cut(s) 295, 320
Hin1II CATG 2 cut(s) 173, 239
HincII GTYRAC 2 cut(s) 7, 257
HindII GTYRAC 2 cut(s) 7, 257
HinfI GANTC 1 cut(s) 191
HphI GGTGA 1 cut(s) 333
Hpy166II GTNNAC 3 cut(s) 7, 182, 257
Hpy188III TCNNGA 2 cut(s) 197, 224
Hpy8I GTNNAC 3 cut(s) 7, 182, 257
HpyAV CCTTC 1 cut(s) 171
HpyCH4V TGCA 4 cut(s) 121, 169, 182, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 2 cut(s) 263, 324
Hsp92II CATG 2 cut(s) 173, 239
Kzo9I GATC 1 cut(s) 157
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 1 cut(s) 137
MaeI CTAG 2 cut(s) 177, 185
MaeIII GTNAC 2 cut(s) 74, 339
MalI GATC 1 cut(s) 159
MboI GATC 1 cut(s) 157
MboII GAAGA 4 cut(s) 70, 124, 200, 319
MhlI GDGCHC 1 cut(s) 184
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 8 cut(s) 10, 26, 33, 63, 95, 200, 228, 367
MluNI TGGCCA 1 cut(s) 320
MnlI CCTC 1 cut(s) 202
Mox20I TGGCCA 1 cut(s) 320
MscI TGGCCA 1 cut(s) 320
MseI TTAA 1 cut(s) 279
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 353
MspR9I CCNGG 2 cut(s) 80, 297
MvaI CCWGG 2 cut(s) 80, 297
MwoI GCNNNNNNNGC 1 cut(s) 353
NcoI CCATGG 1 cut(s) 235
NdeI CATATG 1 cut(s) 123
NdeII GATC 1 cut(s) 157
NlaIII CATG 2 cut(s) 173, 239
NmuCI GTSAC 1 cut(s) 339
PaqCI CACCTGC 1 cut(s) 352
PfeI GAWTC 1 cut(s) 191
PkrI GCNGC 1 cut(s) 355
Psp6I CCWGG 2 cut(s) 78, 295
PspEI GGTNACC 1 cut(s) 339
PspGI CCWGG 2 cut(s) 78, 295
PspPI GGNCC 1 cut(s) 293
PvuII CAGCTG 1 cut(s) 353
SalI GTCGAC 1 cut(s) 255
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 1 cut(s) 354
Sau3AI GATC 1 cut(s) 157
Sau96I GGNCC 1 cut(s) 293
ScrFI CCNGG 2 cut(s) 80, 297
SduI GDGCHC 1 cut(s) 184
SetI ASST 4 cut(s) 43, 178, 346, 355
SfaNI GCATC 1 cut(s) 137
SfcI CTRYAG 1 cut(s) 288
SmlI CTYRAG 1 cut(s) 195
SmoI CTYRAG 1 cut(s) 195
Sse9I AATT 8 cut(s) 10, 26, 33, 63, 95, 200, 228, 367
SspMI CTAG 2 cut(s) 177, 185
StyD4I CCNGG 2 cut(s) 78, 295
StyI CCWWGG 1 cut(s) 235
TaqI TCGA 1 cut(s) 256
TasI AATT 8 cut(s) 10, 26, 33, 63, 95, 200, 228, 367
TfiI GAWTC 1 cut(s) 191
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 1 cut(s) 339
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 1 cut(s) 339
TspDTI ATGAA 2 cut(s) 125, 186
TspRI CASTG 1 cut(s) 328
VneI GTGCAC 1 cut(s) 180
XapI RAATTY 1 cut(s) 200
XmiI GTMKAC 1 cut(s) 256
XspI CTAG 2 cut(s) 177, 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.