Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 583 |
| AciI | CCGC | 1 cut(s) | 140 |
| AclWI | GGATC | 1 cut(s) | 179 |
| AcsI | RAATTY | 1 cut(s) | 580 |
| AcuI | CTGAAG | 1 cut(s) | 368 |
| AfaI | GTAC | 3 cut(s) | 51, 393, 467 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 430, 583 |
| AflII | CTTAAG | 1 cut(s) | 475 |
| AgsI | TTSAA | 1 cut(s) | 67 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 137, 169 |
| AluBI | AGCT | 5 cut(s) | 11, 35, 129, 229, 620 |
| AluI | AGCT | 5 cut(s) | 11, 35, 129, 229, 620 |
| AlwI | GGATC | 1 cut(s) | 179 |
| ApeKI | GCWGC | 2 cut(s) | 126, 319 |
| ApoI | RAATTY | 1 cut(s) | 580 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 371, 403 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 298 |
| AspS9I | GGNCC | 1 cut(s) | 574 |
| AvaII | GGWCC | 1 cut(s) | 574 |
| BbvI | GCAGC | 2 cut(s) | 138, 331 |
| BccI | CCATC | 3 cut(s) | 64, 154, 311 |
| BfrI | CTTAAG | 1 cut(s) | 475 |
| BisI | GCNGC | 2 cut(s) | 127, 320 |
| BlsI | GCNGC | 2 cut(s) | 128, 321 |
| Bme18I | GGWCC | 1 cut(s) | 574 |
| BmgT120I | GGNCC | 1 cut(s) | 574 |
| BmrI | ACTGGG | 1 cut(s) | 177 |
| BmsI | GCATC | 2 cut(s) | 127, 290 |
| BmuI | ACTGGG | 1 cut(s) | 177 |
| BpmI | CTGGAG | 1 cut(s) | 251 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 430, 583 |
| Bse1I | ACTGG | 2 cut(s) | 172, 455 |
| Bse3DI | GCAATG | 1 cut(s) | 597 |
| BseGI | GGATG | 2 cut(s) | 142, 372 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 430, 583 |
| BseMI | GCAATG | 1 cut(s) | 597 |
| BseNI | ACTGG | 2 cut(s) | 172, 455 |
| BseXI | GCAGC | 2 cut(s) | 138, 331 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 430, 583 |
| Bsp1407I | TGTACA | 1 cut(s) | 391 |
| Bsp143I | GATC | 3 cut(s) | 171, 243, 511 |
| BspACI | CCGC | 1 cut(s) | 140 |
| BspPI | GGATC | 1 cut(s) | 179 |
| BspQI | GCTCTTC | 1 cut(s) | 201 |
| BspTI | CTTAAG | 1 cut(s) | 475 |
| BsrDI | GCAATG | 1 cut(s) | 597 |
| BsrGI | TGTACA | 1 cut(s) | 391 |
| BsrI | ACTGG | 2 cut(s) | 172, 455 |
| BssMI | GATC | 3 cut(s) | 171, 243, 511 |
| Bst6I | CTCTTC | 1 cut(s) | 201 |
| BstAFI | CTTAAG | 1 cut(s) | 475 |
| BstAUI | TGTACA | 1 cut(s) | 391 |
| BstF5I | GGATG | 2 cut(s) | 142, 372 |
| BstKTI | GATC | 3 cut(s) | 174, 246, 514 |
| BstMBI | GATC | 3 cut(s) | 171, 243, 511 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 146 |
| BstV1I | GCAGC | 2 cut(s) | 138, 331 |
| BstX2I | RGATCY | 1 cut(s) | 171 |
| BstYI | RGATCY | 1 cut(s) | 171 |
| BtsCI | GGATG | 2 cut(s) | 142, 372 |
| BtsI | GCAGTG | 1 cut(s) | 218 |
| BtsIMutI | CAGTG | 2 cut(s) | 111, 218 |
| Cfr13I | GGNCC | 1 cut(s) | 574 |
| CseI | GACGC | 1 cut(s) | 35 |
| Csp6I | GTAC | 3 cut(s) | 50, 392, 466 |
| CviAII | CATG | 2 cut(s) | 151, 325 |
| CviQI | GTAC | 3 cut(s) | 50, 392, 466 |
| DpnI | GATC | 3 cut(s) | 173, 245, 513 |
| DpnII | GATC | 3 cut(s) | 171, 243, 511 |
| Eam1104I | CTCTTC | 1 cut(s) | 201 |
| EarI | CTCTTC | 1 cut(s) | 201 |
| Eco32I | GATATC | 2 cut(s) | 259, 357 |
| Eco47I | GGWCC | 1 cut(s) | 574 |
| Eco57I | CTGAAG | 1 cut(s) | 368 |
| EcoRV | GATATC | 2 cut(s) | 259, 357 |
| EcoT22I | ATGCAT | 1 cut(s) | 599 |
| FaeI | CATG | 2 cut(s) | 154, 328 |
| FatI | CATG | 2 cut(s) | 150, 324 |
| FauNDI | CATATG | 2 cut(s) | 286, 593 |
| Fnu4HI | GCNGC | 2 cut(s) | 127, 320 |
| FokI | GGATG | 2 cut(s) | 149, 379 |
| Fsp4HI | GCNGC | 2 cut(s) | 127, 320 |
| GluI | GCNGC | 2 cut(s) | 127, 320 |
| GsuI | CTGGAG | 1 cut(s) | 251 |
| HgaI | GACGC | 1 cut(s) | 35 |
| Hin1II | CATG | 2 cut(s) | 154, 328 |
| HinfI | GANTC | 2 cut(s) | 220, 454 |
| Hpy188I | TCNGA | 2 cut(s) | 348, 361 |
| Hpy188III | TCNNGA | 2 cut(s) | 16, 67 |
| HpyAV | CCTTC | 2 cut(s) | 187, 392 |
| HpyCH4V | TGCA | 3 cut(s) | 254, 542, 597 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 146 |
| Hsp92II | CATG | 2 cut(s) | 154, 328 |
| Kzo9I | GATC | 3 cut(s) | 171, 243, 511 |
| LguI | GCTCTTC | 1 cut(s) | 201 |
| LpnPI | CCDG | 5 cut(s) | 153, 215, 359, 436, 590 |
| Lsp1109I | GCAGC | 2 cut(s) | 138, 331 |
| LweI | GCATC | 2 cut(s) | 127, 290 |
| MaeIII | GTNAC | 4 cut(s) | 104, 163, 404, 610 |
| MalI | GATC | 3 cut(s) | 173, 245, 513 |
| MboI | GATC | 3 cut(s) | 171, 243, 511 |
| MboII | GAAGA | 5 cut(s) | 55, 218, 361, 364, 566 |
| MflI | RGATCY | 1 cut(s) | 171 |
| MluCI | AATT | 2 cut(s) | 178, 580 |
| MmeI | TCCRAC | 1 cut(s) | 135 |
| MnlI | CCTC | 4 cut(s) | 127, 271, 424, 456 |
| Mph1103I | ATGCAT | 1 cut(s) | 599 |
| MroXI | GAANNNNTTC | 1 cut(s) | 298 |
| MseI | TTAA | 1 cut(s) | 476 |
| MslI | CAYNNNNRTG | 1 cut(s) | 308 |
| MspA1I | CMGCKG | 1 cut(s) | 229 |
| MspCI | CTTAAG | 1 cut(s) | 475 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 146 |
| NdeI | CATATG | 2 cut(s) | 286, 593 |
| NdeII | GATC | 3 cut(s) | 171, 243, 511 |
| NlaIII | CATG | 2 cut(s) | 154, 328 |
| NmuCI | GTSAC | 1 cut(s) | 404 |
| NsiI | ATGCAT | 1 cut(s) | 599 |
| PciSI | GCTCTTC | 1 cut(s) | 201 |
| PdmI | GAANNNNTTC | 1 cut(s) | 298 |
| PfeI | GAWTC | 2 cut(s) | 220, 454 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 583 |
| PkrI | GCNGC | 2 cut(s) | 128, 321 |
| PspPI | GGNCC | 1 cut(s) | 574 |
| PsuI | RGATCY | 1 cut(s) | 171 |
| PvuII | CAGCTG | 1 cut(s) | 229 |
| RsaI | GTAC | 3 cut(s) | 51, 393, 467 |
| RsaNI | GTAC | 3 cut(s) | 50, 392, 466 |
| RseI | CAYNNNNRTG | 1 cut(s) | 308 |
| SapI | GCTCTTC | 1 cut(s) | 201 |
| SaqAI | TTAA | 1 cut(s) | 476 |
| SatI | GCNGC | 2 cut(s) | 127, 320 |
| Sau3AI | GATC | 3 cut(s) | 171, 243, 511 |
| Sau96I | GGNCC | 1 cut(s) | 574 |
| SetI | ASST | 7 cut(s) | 13, 37, 131, 231, 384, 467, 622 |
| SfaNI | GCATC | 2 cut(s) | 127, 290 |
| SinI | GGWCC | 1 cut(s) | 574 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 308 |
| SmlI | CTYRAG | 1 cut(s) | 475 |
| SmoI | CTYRAG | 1 cut(s) | 475 |
| Sse9I | AATT | 2 cut(s) | 178, 580 |
| SsiI | CCGC | 1 cut(s) | 140 |
| TaqI | TCGA | 2 cut(s) | 306, 510 |
| TasI | AATT | 2 cut(s) | 178, 580 |
| TatI | WGTACW | 1 cut(s) | 391 |
| TfiI | GAWTC | 2 cut(s) | 220, 454 |
| Tru1I | TTAA | 1 cut(s) | 476 |
| Tru9I | TTAA | 1 cut(s) | 476 |
| TscAI | CASTG | 2 cut(s) | 118, 218 |
| TseFI | GTSAC | 1 cut(s) | 404 |
| TseI | GCWGC | 2 cut(s) | 126, 319 |
| Tsp45I | GTSAC | 1 cut(s) | 404 |
| TspDTI | ATGAA | 4 cut(s) | 60, 341, 383, 446 |
| TspGWI | ACGGA | 1 cut(s) | 306 |
| TspRI | CASTG | 2 cut(s) | 118, 218 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 583 |
| Vha464I | CTTAAG | 1 cut(s) | 475 |
| VpaK11BI | GGWCC | 1 cut(s) | 574 |
| XapI | RAATTY | 1 cut(s) | 580 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 157 |
| XmnI | GAANNNNTTC | 1 cut(s) | 298 |
| Zsp2I | ATGCAT | 1 cut(s) | 599 |