RLG00000000628

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001161
Physical Location & Seq
Forward (+)
2754921 .. 2757813
2893 bp
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UTR
Exon/CDS
Intron
RLM00000000628

Sequence Viewer

Length: 369 bp
ATGCTCAAGGAGAAGTTAATTGAGAGCCTTTTGCCCATGCTGCTTGACAATTTTGATAGAGCAAAAGTACAAATTAGACCTGAAACTGAAAATGAGAAGAAAATTGATACAAGTTACAGGGGCATATACAAGCAATTTGTTGAGATTATGAGGAGTTTGCGTGTGGCATTTGTATCAACTATGGGAAAACCCTTTGATCCTTCGGATTGGAAGGCTGGGATGAGAGTCAAGATGGCCATGGAGACTGATGACTCCTCCAGGATGACTTGGTTTCAGGGCACTCTGTCTTCCGCCTTTGTTCCGGAGAATGGGCCTTGGAAGGGATCTCCATGGCGTATGCTTCAGGCTCTCAAGTCTGGCAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

123

Amino Acids

14.11

Weight (kDa)

9.64

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 4 - 77 2.1e-07 GrpE
ARF_AD PF06507 69 - 115 9e-13 Auxin response factor ancillary domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 1 cut(s) 291
AclWI GGATC 2 cut(s) 191, 331
AcoI YGGCCR 1 cut(s) 234
AcuI CTGAAG 1 cut(s) 326
AfaI GTAC 1 cut(s) 69
AfiI CCNNNNNNNGG 2 cut(s) 308, 320
AjnI CCWGG 1 cut(s) 257
AluBI AGCT 1 cut(s) 366
AluI AGCT 1 cut(s) 366
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 2 cut(s) 191, 331
AlwNI CAGNNNCTG 1 cut(s) 366
Aor13HI TCCGGA 1 cut(s) 301
AoxI GGCC 2 cut(s) 234, 311
ApeKI GCWGC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 311
BaeGI GKGCMC 1 cut(s) 281
BalI TGGCCA 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 279
BbvI GCAGC 1 cut(s) 27
BccI CCATC 1 cut(s) 226
BciT130I CCWGG 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 236
BisI GCNGC 1 cut(s) 41
BlsI GCNGC 1 cut(s) 42
Bme1390I CCNGG 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 311
BmrFI CCNGG 1 cut(s) 259
BpiI GAAGAC 1 cut(s) 279
BpmI CTGGAG 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 335
BsaJI CCNNGG 3 cut(s) 237, 314, 329
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 2 cut(s) 308, 320
BseAI TCCGGA 1 cut(s) 301
BseBI CCWGG 1 cut(s) 259
BseDI CCNNGG 3 cut(s) 237, 314, 329
BseGI GGATG 2 cut(s) 225, 267
BseLI CCNNNNNNNGG 2 cut(s) 308, 320
BseRI GAGGAG 2 cut(s) 166, 244
BseSI GKGCMC 1 cut(s) 281
BseXI GCAGC 1 cut(s) 27
BseYI CCCAGC 1 cut(s) 215
BshFI GGCC 2 cut(s) 236, 313
BsiSI CCGG 1 cut(s) 302
BslI CCNNNNNNNGG 2 cut(s) 308, 320
BsmAI GTCTC 1 cut(s) 236
BsnI GGCC 2 cut(s) 236, 313
Bsp1286I GDGCHC 1 cut(s) 281
Bsp13I TCCGGA 1 cut(s) 301
Bsp143I GATC 2 cut(s) 196, 323
Bsp19I CCATGG 2 cut(s) 237, 329
BspACI CCGC 1 cut(s) 291
BspANI GGCC 2 cut(s) 236, 313
BspEI TCCGGA 1 cut(s) 301
BspPI GGATC 2 cut(s) 191, 331
BssECI CCNNGG 3 cut(s) 237, 314, 329
BssMI GATC 2 cut(s) 196, 323
BssT1I CCWWGG 3 cut(s) 237, 314, 329
Bst2UI CCWGG 1 cut(s) 259
BstDSI CCRYGG 2 cut(s) 237, 329
BstF5I GGATG 2 cut(s) 225, 267
BstKTI GATC 2 cut(s) 199, 326
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 2 cut(s) 196, 323
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstNI CCWGG 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 257
BstSLI GKGCMC 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 27
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 1 cut(s) 323
BstYI RGATCY 1 cut(s) 323
BsuRI GGCC 2 cut(s) 236, 313
BtgI CCRYGG 2 cut(s) 237, 329
BtsCI GGATG 2 cut(s) 225, 267
CaiI CAGNNNCTG 1 cut(s) 366
Cfr13I GGNCC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 68
CviAII CATG 3 cut(s) 37, 238, 330
CviJI RGCY 6 cut(s) 27, 215, 236, 313, 347, 366
CviKI_1 RGCY 6 cut(s) 27, 215, 236, 313, 347, 366
CviQI GTAC 1 cut(s) 68
DpnI GATC 2 cut(s) 198, 325
DpnII GATC 2 cut(s) 196, 323
EaeI YGGCCR 1 cut(s) 234
EciI GGCGGA 1 cut(s) 280
Eco130I CCWWGG 3 cut(s) 237, 314, 329
Eco57I CTGAAG 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 257
EcoT14I CCWWGG 3 cut(s) 237, 314, 329
ErhI CCWWGG 3 cut(s) 237, 314, 329
FaeI CATG 3 cut(s) 40, 241, 333
FaiI YATR 8 cut(s) 38, 125, 127, 149, 182, 239, 331, 338
FatI CATG 3 cut(s) 36, 237, 329
Fnu4HI GCNGC 1 cut(s) 41
FokI GGATG 2 cut(s) 232, 274
Fsp4HI GCNGC 1 cut(s) 41
GluI GCNGC 1 cut(s) 41
GsaI CCCAGC 1 cut(s) 219
GsuI CTGGAG 1 cut(s) 241
HaeIII GGCC 2 cut(s) 236, 313
HapII CCGG 1 cut(s) 302
Hin1II CATG 3 cut(s) 40, 241, 333
HinfI GANTC 2 cut(s) 225, 251
HpaII CCGG 1 cut(s) 302
Hpy188I TCNGA 1 cut(s) 205
Hpy188III TCNNGA 2 cut(s) 229, 302
HpyAV CCTTC 3 cut(s) 205, 210, 313
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
Hsp92II CATG 3 cut(s) 40, 241, 333
Kpn2I TCCGGA 1 cut(s) 301
Kzo9I GATC 2 cut(s) 196, 323
LpnPI CCDG 9 cut(s) 93, 103, 201, 244, 260, 271, 315, 329, 342
Lsp1109I GCAGC 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 113
MalI GATC 2 cut(s) 198, 325
MboI GATC 2 cut(s) 196, 323
MboII GAAGA 2 cut(s) 109, 279
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 1 cut(s) 281
MlsI TGGCCA 1 cut(s) 236
MluCI AATT 5 cut(s) 18, 49, 72, 102, 134
MluNI TGGCCA 1 cut(s) 236
MlyI GAGTC 2 cut(s) 234, 245
MnlI CCTC 2 cut(s) 144, 265
Mox20I TGGCCA 1 cut(s) 236
MroI TCCGGA 1 cut(s) 301
MscI TGGCCA 1 cut(s) 236
MseI TTAA 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 236
MspI CCGG 1 cut(s) 302
MspR9I CCNGG 1 cut(s) 259
MvaI CCWGG 1 cut(s) 259
MwoI GCNNNNNNNGC 1 cut(s) 40
NcoI CCATGG 2 cut(s) 237, 329
NdeII GATC 2 cut(s) 196, 323
NlaIII CATG 3 cut(s) 40, 241, 333
PfoI TCCNGGA 1 cut(s) 257
PkrI GCNGC 1 cut(s) 42
PleI GAGTC 2 cut(s) 233, 245
PpsI GAGTC 2 cut(s) 233, 245
Psp6I CCWGG 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 215
PspGI CCWGG 1 cut(s) 257
PspPI GGNCC 1 cut(s) 311
PstNI CAGNNNCTG 1 cut(s) 366
PsuI RGATCY 1 cut(s) 323
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 1 cut(s) 41
Sau3AI GATC 2 cut(s) 196, 323
Sau96I GGNCC 1 cut(s) 311
SchI GAGTC 2 cut(s) 234, 245
ScrFI CCNGG 1 cut(s) 259
SduI GDGCHC 1 cut(s) 281
SetI ASST 2 cut(s) 82, 368
SmlI CTYRAG 2 cut(s) 5, 350
SmoI CTYRAG 2 cut(s) 5, 350
Sse9I AATT 5 cut(s) 18, 49, 72, 102, 134
SsiI CCGC 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 257
StyI CCWWGG 3 cut(s) 237, 314, 329
TasI AATT 5 cut(s) 18, 49, 72, 102, 134
TatI WGTACW 1 cut(s) 67
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TseI GCWGC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.