Rh7AG492700

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001161
Physical Location & Seq
Reverse (-)
69301660 .. 69310483
8824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG492700.1

Sequence Viewer

Length: 675 bp
ATGACTTTAGTTTGTGCTGATTTGGTTCGCGGTGCCAAGGACAAGAGGCTTAGGGTGAAGGGACCAGTGAGGATGCCTACCAAGGTTCTGCACATTACCACCAGGAAGTCTCCCTGTGGAGAAGATCACCTCCAGTCCTCCATCACGATTGAACCCGGTGTTGAAGTGGAGGGAGGTTGCAGGGACGGTTTTGCTGTTATGGCTGGTGTCAATGCTAGGATATCTTATGTCATGATTAAATCCTTCATAGCGGTATCCCATCTCAGGTCATGGACTAAACTTGGAGTTATGACAGCTTCTCAACGCAATATAAGCTTAGATGCTTTTTATAAGTCATTGTGCAACATTAATATGTTTTTGATGGTTGATATGATTTCCTTCTGCATCAGGCTATGTCAAGAATGCCAAACTTTGATTGAAGACCATGATAAGATAAAGCTTCTCAGCAATGCAAGGAATAACTTGAATACAACCTTAAAGGTCAATGCCAAAGAGGATAATGTAGAAACCAGTGGAGCAGATCAACAACAATTGCCCAGTTTGAGAACTCTCCTTAAAGTTTACAAGGATGCTATTTTCAATGAAGATGAAAAGACTGTATCTGAAATTGAGGCAAGGATAGAAGTAATAGAAAATAAGCAACATGAATTAGTCCAGAAAGTATCATCTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

224

Amino Acids

25.14

Weight (kDa)

8.44

Isoelectric Point (pI)

49.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S10 PF00338 4 - 46 1.7e-07 Ribosomal protein S10p/S20e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 330
AccB1I GGYRCC 1 cut(s) 32
AccII CGCG 1 cut(s) 30
AciI CCGC 2 cut(s) 30, 251
AfiI CCNNNNNNNGG 1 cut(s) 264
AgsI TTSAA 5 cut(s) 152, 164, 419, 466, 580
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 3 cut(s) 296, 315, 439
AluI AGCT 3 cut(s) 296, 315, 439
Alw26I GTCTC 1 cut(s) 114
AseI ATTAAT 1 cut(s) 348
AspS9I GGNCC 1 cut(s) 62
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 2 cut(s) 67, 119
AvaII GGWCC 1 cut(s) 62
BanI GGYRCC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 426
BccI CCATC 3 cut(s) 149, 267, 355
BciT130I CCWGG 1 cut(s) 103
BciVI GTATCC 1 cut(s) 265
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 1 cut(s) 216
BfuI GTATCC 1 cut(s) 265
Bme1390I CCNGG 2 cut(s) 103, 156
Bme18I GGWCC 1 cut(s) 62
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 2 cut(s) 34, 63
BmrFI CCNGG 2 cut(s) 103, 156
BmrI ACTGGG 1 cut(s) 531
BmsI GCATC 4 cut(s) 63, 310, 393, 559
BmuI ACTGGG 1 cut(s) 531
BpiI GAAGAC 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 156
BsaJI CCNNGG 2 cut(s) 36, 81
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse1I ACTGG 4 cut(s) 65, 133, 510, 537
Bse3DI GCAATG 1 cut(s) 454
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 2 cut(s) 36, 81
BseGI GGATG 2 cut(s) 78, 574
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMI GCAATG 1 cut(s) 454
BseMII CTCAG 2 cut(s) 277, 457
BseNI ACTGG 4 cut(s) 65, 133, 510, 537
BsgI GTGCAG 1 cut(s) 74
Bsh1236I CGCG 1 cut(s) 30
BshNI GGYRCC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 2 cut(s) 75, 197
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 2 cut(s) 75, 197
BsmI GAATGC 1 cut(s) 407
Bsp143I GATC 2 cut(s) 124, 520
BspACI CCGC 2 cut(s) 30, 251
BspCNI CTCAG 2 cut(s) 276, 456
BspFNI CGCG 1 cut(s) 30
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 2 cut(s) 34, 63
BspT107I GGYRCC 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 454
BsrI ACTGG 4 cut(s) 65, 133, 510, 537
BssECI CCNNGG 2 cut(s) 36, 81
BssMI GATC 2 cut(s) 124, 520
BssT1I CCWWGG 2 cut(s) 36, 81
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 2 cut(s) 188, 598
BstDEI CTNAG 4 cut(s) 50, 263, 316, 443
BstF5I GGATG 2 cut(s) 78, 574
BstFNI CGCG 1 cut(s) 30
BstKTI GATC 2 cut(s) 127, 523
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 2 cut(s) 124, 520
BstMWI GCNNNNNNNGC 2 cut(s) 200, 312
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 2 cut(s) 101, 154
BstUI CGCG 1 cut(s) 30
BstV2I GAAGAC 1 cut(s) 426
BsuI GTATCC 1 cut(s) 265
BtsCI GGATG 2 cut(s) 78, 574
BtsIMutI CAGTG 2 cut(s) 72, 517
CciI TCATGA 1 cut(s) 231
Cfr13I GGNCC 1 cut(s) 62
CviAII CATG 4 cut(s) 232, 270, 425, 644
CviJI RGCY 6 cut(s) 49, 203, 296, 315, 391, 439
CviKI_1 RGCY 6 cut(s) 49, 203, 296, 315, 391, 439
DdeI CTNAG 4 cut(s) 50, 263, 316, 443
DpnI GATC 2 cut(s) 126, 522
DpnII GATC 2 cut(s) 124, 520
Eco130I CCWWGG 2 cut(s) 36, 81
Eco32I GATATC 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 101
EcoRV GATATC 1 cut(s) 222
EcoT14I CCWWGG 2 cut(s) 36, 81
ErhI CCWWGG 2 cut(s) 36, 81
FaeI CATG 4 cut(s) 235, 273, 428, 647
FaqI GGGAC 2 cut(s) 75, 197
FatI CATG 4 cut(s) 231, 269, 424, 643
FokI GGATG 2 cut(s) 85, 581
FspBI CTAG 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 116
HapII CCGG 1 cut(s) 156
Hin1II CATG 4 cut(s) 235, 273, 428, 647
HindIII AAGCTT 2 cut(s) 313, 437
HpaII CCGG 1 cut(s) 156
HphI GGTGA 2 cut(s) 67, 119
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 1 cut(s) 604
Hpy188III TCNNGA 4 cut(s) 145, 232, 398, 655
Hpy8I GTNNAC 1 cut(s) 562
HpyAV CCTTC 3 cut(s) 52, 253, 388
HpyCH4III ACNGT 2 cut(s) 188, 598
HpyCH4V TGCA 5 cut(s) 91, 180, 342, 384, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 312
HpyF3I CTNAG 4 cut(s) 50, 263, 316, 443
Hsp92II CATG 4 cut(s) 235, 273, 428, 647
Kzo9I GATC 2 cut(s) 124, 520
LmnI GCTCC 1 cut(s) 515
LweI GCATC 4 cut(s) 63, 310, 393, 559
MaeI CTAG 1 cut(s) 216
MalI GATC 2 cut(s) 126, 522
MboI GATC 2 cut(s) 124, 520
MboII GAAGA 3 cut(s) 134, 431, 596
MfeI CAATTG 1 cut(s) 530
MluCI AATT 3 cut(s) 530, 606, 647
MnlI CCTC 8 cut(s) 39, 63, 140, 148, 163, 167, 487, 604
MseI TTAA 4 cut(s) 237, 348, 476, 555
MslI CAYNNNNRTG 1 cut(s) 350
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 2 cut(s) 103, 156
MunI CAATTG 1 cut(s) 530
Mva1269I GAATGC 1 cut(s) 407
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 2 cut(s) 200, 312
NciI CCSGG 1 cut(s) 156
NdeII GATC 2 cut(s) 124, 520
NlaIII CATG 4 cut(s) 235, 273, 428, 647
NlaIV GGNNCC 2 cut(s) 34, 63
PagI TCATGA 1 cut(s) 231
PctI GAATGC 1 cut(s) 407
PshBI ATTAAT 1 cut(s) 348
PsiI TTATAA 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 34, 63
PspPI GGNCC 1 cut(s) 62
RseI CAYNNNNRTG 1 cut(s) 350
SaqAI TTAA 4 cut(s) 237, 348, 476, 555
Sau3AI GATC 2 cut(s) 124, 520
Sau96I GGNCC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 103, 156
SetI ASST 9 cut(s) 87, 132, 178, 269, 298, 317, 441, 476, 483
SfaNI GCATC 4 cut(s) 63, 310, 393, 559
SinI GGWCC 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 350
Sse9I AATT 3 cut(s) 530, 606, 647
SsiI CCGC 2 cut(s) 30, 251
SspMI CTAG 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 101, 154
StyI CCWWGG 2 cut(s) 36, 81
TaaI ACNGT 2 cut(s) 188, 598
TasI AATT 3 cut(s) 530, 606, 647
Tru1I TTAA 4 cut(s) 237, 348, 476, 555
Tru9I TTAA 4 cut(s) 237, 348, 476, 555
TscAI CASTG 2 cut(s) 72, 517
TspDTI ATGAA 4 cut(s) 235, 597, 603, 660
TspRI CASTG 2 cut(s) 72, 517
VpaK11BI GGWCC 1 cut(s) 62
VspI ATTAAT 1 cut(s) 348
XspI CTAG 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.