Rh7DG475500

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001161
Physical Location & Seq
Reverse (-)
67928386 .. 67954531
26146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG475500.1

Sequence Viewer

Length: 1125 bp
ATGGTCATGGGTTTAGCTTTAAGCTTAGTTTTGGTACAACAGGTGAATGCAAAAGAGGATAATGTAGATTCTAGTGGAGCAGATCAACAACATTTGCCCAGCTTGAGGACCCTCCTTAAAGTTTACAAGGATGCTATTTTCAATGAAGATGAAAAGACTGTATCCGAAATTGAGGCAAGGATAGAAGTAATAGAAAATAAGCAAAATGAATTAGTCCAGAAAGTATCATCTATATCTGCAGAGGTAACATCAGGGAAGCAAAAGCTTATCCGCTTGCAAGCAGATTTTGATAATTGTAGAAAAAGATTTGAGAAAGAGAGACTTACCGTTAGGAGTCTAAGTAGGACTGATGCTCAAGGAGAAGTAATTGAGAGCCTTTTGGCCATGGTTGACAATTTTGAGAGAGCAGAAGTACAAATCAGACCTGAACCCGAAAATGAGAAGAAAATTGATACAAGTTACCAGGGAGTGCCCAACACATTGAATGTGATCACCAATTTCCCATTCTTGGTTGTTGGGGTTCTGGGTTTTGTTCTCTGTGTCCAAGGAGGCTTTTTCAATATCAGTTTGCCAGGTGAAGTTTGGGGTTGGGCACTGTTCTATGCAGGAATAGCAGGGCTGACTTTTGGCTCTGCTTATTATCATTTGAAGCCTGATGATAGTAGAGTGACTTGGGATACCTTGCCGGCTAGCATTTCAATCCATCGTTCTTCGGAGGTGTGGGAGAGAGCAGAAGAATTTTCACCAGAGAGATTTGATATGGAAGGTCCTATACCCAATGAAACGAATACAGATTTCAGGTTCATTCCATTCAGTGGAGGGCCACGAAAACATTTGAATAGCAATAGATTGAGTAGGCAAATTCCGCCTTCAATTGGTAGTCTTTCGAATTTTTTCTATCTGGACCTAATTGGCAACAAACTTCAAGGATACATCGCAGTCTATAGTGAGGCTACACCGAGTCTTGATATTCATCTAGGAGACAATGCTCTCAGGCGGAATTCAACTCAACTTTTCAGCTCAAAGATGGTTCTAGTGCACTTGTTTGGTAATTCATATGGTATTGCTGGTAGAAGTGAGAACAGGAGCAAGGGTAGAAGCTTGGGAACTTTGGAAAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

374

Amino Acids

41.92

Weight (kDa)

5.68

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 64 - 163 5.2e-09 GrpE
p450 PF00067 231 - 276 2.3e-08 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 815
AciI CCGC 3 cut(s) 271, 866, 997
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 4 cut(s) 737, 861, 889, 1000
AfaI GTAC 2 cut(s) 36, 414
AfiI CCNNNNNNNGG 4 cut(s) 105, 508, 815, 875
AgsI TTSAA 9 cut(s) 142, 484, 559, 649, 699, 838, 873, 926, 1005
AjnI CCWGG 2 cut(s) 462, 571
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AluBI AGCT 6 cut(s) 17, 24, 102, 265, 1020, 1101
AluI AGCT 6 cut(s) 17, 24, 102, 265, 1020, 1101
Alw21I GWGCWC 1 cut(s) 1041
Alw26I GTCTC 2 cut(s) 313, 975
Alw44I GTGCAC 1 cut(s) 1037
AoxI GGCC 2 cut(s) 381, 821
ApaLI GTGCAC 1 cut(s) 1037
ApoI RAATTY 4 cut(s) 737, 861, 889, 1000
Asp700I GAANNNNTTC 1 cut(s) 893
AspS9I GGNCC 4 cut(s) 108, 767, 821, 904
AsuHPI GGTGA 4 cut(s) 55, 484, 587, 735
AsuII TTCGAA 1 cut(s) 887
AsuNHI GCTAGC 1 cut(s) 689
AvaII GGWCC 3 cut(s) 108, 767, 904
BaeGI GKGCMC 3 cut(s) 474, 595, 1041
BalI TGGCCA 1 cut(s) 383
BarI GAAGNNNNNNTAC 2 cut(s) 756, 788
Bbv12I GWGCWC 1 cut(s) 1041
BccI CCATC 2 cut(s) 711, 1021
BciT130I CCWGG 2 cut(s) 464, 573
BciVI GTATCC 3 cut(s) 172, 670, 923
BclI TGATCA 1 cut(s) 489
BcoDI GTCTC 2 cut(s) 313, 975
BfaI CTAG 4 cut(s) 72, 690, 977, 1034
BfmI CTRYAG 2 cut(s) 237, 943
BfuI GTATCC 3 cut(s) 172, 670, 923
Bme1390I CCNGG 2 cut(s) 464, 573
Bme18I GGWCC 3 cut(s) 108, 767, 904
BmgT120I GGNCC 4 cut(s) 108, 767, 821, 904
BmiI GGNNCC 1 cut(s) 110
BmrFI CCNGG 2 cut(s) 464, 573
BmsI GCATC 2 cut(s) 121, 340
BmtI GCTAGC 1 cut(s) 693
Bpu14I TTCGAA 1 cut(s) 887
BpuEI CTTGAG 2 cut(s) 124, 339
BsaBI GATNNNNATC 1 cut(s) 972
BsaJI CCNNGG 3 cut(s) 384, 463, 544
BsaXI ACNNNNNCTCC 4 cut(s) 325, 355, 1078, 1108
Bsc4I CCNNNNNNNGG 4 cut(s) 105, 508, 815, 875
Bse118I RCCGGY 1 cut(s) 685
Bse8I GATNNNNATC 1 cut(s) 972
BseBI CCWGG 2 cut(s) 464, 573
BseDI CCNNGG 3 cut(s) 384, 463, 544
BseGI GGATG 1 cut(s) 136
BseJI GATNNNNATC 1 cut(s) 972
BseLI CCNNNNNNNGG 4 cut(s) 105, 508, 815, 875
BseMII CTCAG 1 cut(s) 1006
BseSI GKGCMC 3 cut(s) 474, 595, 1041
BseYI CCCAGC 1 cut(s) 98
BshFI GGCC 2 cut(s) 383, 823
BsiHKAI GWGCWC 1 cut(s) 1041
BsiSI CCGG 1 cut(s) 686
BslI CCNNNNNNNGG 4 cut(s) 105, 508, 815, 875
BsmAI GTCTC 2 cut(s) 313, 975
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 2 cut(s) 383, 823
Bsp119I TTCGAA 1 cut(s) 887
Bsp1286I GDGCHC 3 cut(s) 474, 595, 1041
Bsp143I GATC 2 cut(s) 82, 489
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 3 cut(s) 271, 866, 997
BspANI GGCC 2 cut(s) 383, 823
BspCNI CTCAG 1 cut(s) 1005
BspLI GGNNCC 1 cut(s) 110
BspMAI CTGCAG 1 cut(s) 241
BspOI GCTAGC 1 cut(s) 693
BspT104I TTCGAA 1 cut(s) 887
BsrFI RCCGGY 1 cut(s) 685
BssAI RCCGGY 1 cut(s) 685
BssECI CCNNGG 3 cut(s) 384, 463, 544
BssMI GATC 2 cut(s) 82, 489
BssT1I CCWWGG 2 cut(s) 384, 544
Bst2UI CCWGG 2 cut(s) 464, 573
Bst4CI ACNGT 3 cut(s) 160, 328, 597
BstBI TTCGAA 1 cut(s) 887
BstC8I GCNNGC 4 cut(s) 275, 279, 687, 691
BstDEI CTNAG 3 cut(s) 25, 338, 992
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 136
BstKTI GATC 2 cut(s) 85, 492
BstMAI GTCTC 2 cut(s) 313, 975
BstMBI GATC 2 cut(s) 82, 489
BstMWI GCNNNNNNNGC 2 cut(s) 611, 865
BstNI CCWGG 2 cut(s) 464, 573
BstSCI CCNGG 2 cut(s) 462, 571
BstSFI CTRYAG 2 cut(s) 237, 943
BstSLI GKGCMC 3 cut(s) 474, 595, 1041
BsuI GTATCC 3 cut(s) 172, 670, 923
BsuRI GGCC 2 cut(s) 383, 823
BtgI CCRYGG 1 cut(s) 384
BtgZI GCGATG 1 cut(s) 919
BtsCI GGATG 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 593, 820
Cac8I GCNNGC 4 cut(s) 275, 279, 687, 691
Cfr10I RCCGGY 1 cut(s) 685
Cfr13I GGNCC 4 cut(s) 108, 767, 821, 904
Csp6I GTAC 2 cut(s) 35, 413
CviAII CATG 2 cut(s) 7, 385
CviQI GTAC 2 cut(s) 35, 413
DdeI CTNAG 3 cut(s) 25, 338, 992
DpnI GATC 2 cut(s) 84, 491
DpnII GATC 2 cut(s) 82, 489
EaeI YGGCCR 1 cut(s) 381
EciI GGCGGA 2 cut(s) 855, 1012
Eco130I CCWWGG 2 cut(s) 384, 544
Eco47I GGWCC 3 cut(s) 108, 767, 904
EcoO109I RGGNCCY 2 cut(s) 108, 767
EcoRI GAATTC 1 cut(s) 1000
EcoRII CCWGG 2 cut(s) 462, 571
EcoT14I CCWWGG 2 cut(s) 384, 544
ErhI CCWWGG 2 cut(s) 384, 544
FaeI CATG 2 cut(s) 10, 388
FalI AAGNNNNNCTT 2 cut(s) 306, 338
FatI CATG 2 cut(s) 6, 384
FauNDI CATATG 1 cut(s) 1057
FbaI TGATCA 1 cut(s) 489
FokI GGATG 1 cut(s) 143
FspBI CTAG 4 cut(s) 72, 690, 977, 1034
GsaI CCCAGC 1 cut(s) 102
HaeIII GGCC 2 cut(s) 383, 823
HapII CCGG 1 cut(s) 686
Hin1II CATG 2 cut(s) 10, 388
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HindIII AAGCTT 3 cut(s) 22, 263, 1099
HinfI GANTC 3 cut(s) 68, 334, 961
HpaII CCGG 1 cut(s) 686
HphI GGTGA 4 cut(s) 55, 484, 587, 735
Hpy166II GTNNAC 3 cut(s) 124, 391, 1039
Hpy188I TCNGA 3 cut(s) 166, 422, 715
Hpy188III TCNNGA 3 cut(s) 217, 902, 965
Hpy8I GTNNAC 3 cut(s) 124, 391, 1039
HpyAV CCTTC 2 cut(s) 758, 879
HpyCH4III ACNGT 3 cut(s) 160, 328, 597
HpyCH4V TGCA 5 cut(s) 50, 239, 277, 605, 1039
HpyF10VI GCNNNNNNNGC 2 cut(s) 611, 865
HpyF3I CTNAG 3 cut(s) 25, 338, 992
Hsp92II CATG 2 cut(s) 10, 388
KroI GCCGGC 1 cut(s) 685
KroNI GCCGGC 1 cut(s) 687
Ksp22I TGATCA 1 cut(s) 489
Kzo9I GATC 2 cut(s) 82, 489
LmnI GCTCC 2 cut(s) 77, 1086
LweI GCATC 2 cut(s) 121, 340
MaeI CTAG 4 cut(s) 72, 690, 977, 1034
MaeIII GTNAC 3 cut(s) 244, 458, 667
MalI GATC 2 cut(s) 84, 491
MboI GATC 2 cut(s) 82, 489
MboII GAAGA 4 cut(s) 158, 454, 702, 746
MfeI CAATTG 1 cut(s) 873
MhlI GDGCHC 3 cut(s) 474, 595, 1041
MlsI TGGCCA 1 cut(s) 383
MluNI TGGCCA 1 cut(s) 383
MlyI GAGTC 2 cut(s) 343, 970
MnlI CCTC 9 cut(s) 49, 99, 122, 166, 235, 542, 709, 812, 943
Mox20I TGGCCA 1 cut(s) 383
MroNI GCCGGC 1 cut(s) 685
MroXI GAANNNNTTC 1 cut(s) 893
MscI TGGCCA 1 cut(s) 383
MseI TTAA 2 cut(s) 20, 117
Msp20I TGGCCA 1 cut(s) 383
MspI CCGG 1 cut(s) 686
MspR9I CCNGG 2 cut(s) 464, 573
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 52
MvaI CCWGG 2 cut(s) 464, 573
MwoI GCNNNNNNNGC 2 cut(s) 611, 865
NaeI GCCGGC 1 cut(s) 687
NcoI CCATGG 1 cut(s) 384
NdeI CATATG 1 cut(s) 1057
NdeII GATC 2 cut(s) 82, 489
NgoMIV GCCGGC 1 cut(s) 685
NheI GCTAGC 1 cut(s) 689
NlaIII CATG 2 cut(s) 10, 388
NlaIV GGNNCC 1 cut(s) 110
NmuCI GTSAC 1 cut(s) 667
NspV TTCGAA 1 cut(s) 887
PctI GAATGC 1 cut(s) 52
PdiI GCCGGC 1 cut(s) 687
PdmI GAANNNNTTC 1 cut(s) 893
PfeI GAWTC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 815
PleI GAGTC 2 cut(s) 342, 969
PpsI GAGTC 2 cut(s) 342, 969
PpuMI RGGWCCY 2 cut(s) 108, 767
Psp5II RGGWCCY 2 cut(s) 108, 767
Psp6I CCWGG 2 cut(s) 462, 571
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 2 cut(s) 462, 571
PspN4I GGNNCC 1 cut(s) 110
PspPI GGNCC 4 cut(s) 108, 767, 821, 904
PspPPI RGGWCCY 2 cut(s) 108, 767
PstI CTGCAG 1 cut(s) 241
RsaI GTAC 2 cut(s) 36, 414
RsaNI GTAC 2 cut(s) 35, 413
SaqAI TTAA 2 cut(s) 20, 117
Sau3AI GATC 2 cut(s) 82, 489
Sau96I GGNCC 4 cut(s) 108, 767, 821, 904
SchI GAGTC 2 cut(s) 343, 970
ScrFI CCNGG 2 cut(s) 464, 573
SduI GDGCHC 3 cut(s) 474, 595, 1041
SfaNI GCATC 2 cut(s) 121, 340
SfcI CTRYAG 2 cut(s) 237, 943
SfuI TTCGAA 1 cut(s) 887
SinI GGWCC 3 cut(s) 108, 767, 904
SmlI CTYRAG 2 cut(s) 103, 354
SmoI CTYRAG 2 cut(s) 103, 354
SsiI CCGC 3 cut(s) 271, 866, 997
SspMI CTAG 4 cut(s) 72, 690, 977, 1034
StyD4I CCNGG 2 cut(s) 462, 571
StyI CCWWGG 2 cut(s) 384, 544
TaaI ACNGT 3 cut(s) 160, 328, 597
TaqI TCGA 1 cut(s) 887
TatI WGTACW 1 cut(s) 412
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 2 cut(s) 20, 117
Tru9I TTAA 2 cut(s) 20, 117
TscAI CASTG 2 cut(s) 600, 820
TseFI GTSAC 1 cut(s) 667
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 7 cut(s) 159, 165, 222, 793, 795, 962, 1044
TspRI CASTG 2 cut(s) 600, 820
Van91I CCANNNNNTGG 1 cut(s) 815
VneI GTGCAC 1 cut(s) 1037
VpaK11BI GGWCC 3 cut(s) 108, 767, 904
XapI RAATTY 4 cut(s) 737, 861, 889, 1000
XcmI CCANNNNNNNNNTGG 1 cut(s) 579
XmnI GAANNNNTTC 1 cut(s) 893
XspI CTAG 4 cut(s) 72, 690, 977, 1034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.