Rw0G013470

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00606
OG0001161
Physical Location & Seq
Reverse (-)
2553 .. 5519
2967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G013470.1

Sequence Viewer

Length: 762 bp
ATGGCGAACTCGTTGTTTATCTCAAACAACTACTTCGTCATACCTCCTCCTTGCGTTTCTGCTTCCTTCTCCTCGAAACCCATCTCTTCCCCCTTAGTTTTACATAGCCGCAGCAGCAGCAGCAGGCGTGCCTCGTTTTTCAAATCTCATCTTTCTGCTCGAGGTTCTGTTCCCCCAGTGAATGCCAAAGAGGATAATGTAGATTCTAGTGGAGCAGTTCAACAACATTTGCCCAGTTTGAGGACCCTCCTTGAAGTTTACAAGGATGCTATTTTCAATGAAGATGAAAAGACTGGAAGGAAAAGATCATTCGCCTGCAAGCAGATTTTGATAATTGTAGAAAAAGATTTGAGAAAGAGAGACTTACCATTAGGACTGATGCTCAAGGAGAAGTTAATTGAGAGCCTTTTGCCCATGGTTGACAATTTTGAGAGAGCAAAAGTACAAATTAGACCTGAAACTGAAAATGAGAAGAAAATTGATACAAGTTACAGGGGCATATACAAGCAATTTGTTGCGATTATGAGGAGTTTGCGTGTGGCATCTGTATCAACTATGGGAAAACCGTTTGATCCTTCGGTAATCTCAAGAATTCCCATAGGGGATTGTCATTCAAGAAATTCTCCGTGGCTTTTACTTGGTGGTCGACTTCTAGGACCAGCAATGGTTAAAGTCTCTATAGGGCCTGGCAGTAAGAAGACCCCTGTGGCCACTGAGAAATCATCAGGGTCACCTGCTACACCTGCTTCGTTAGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

253

Amino Acids

27.81

Weight (kDa)

9.87

Isoelectric Point (pI)

53.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 130 - 195 3.8e-09 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 742, 751
Acc36I ACCTGC 2 cut(s) 742, 751
AccI GTMKAC 1 cut(s) 646
AciI CCGC 1 cut(s) 109
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 708
AcsI RAATTY 2 cut(s) 591, 619
AfaI GTAC 1 cut(s) 444
AfiI CCNNNNNNNGG 1 cut(s) 240
AgsI TTSAA 5 cut(s) 142, 221, 254, 277, 615
AjnI CCWGG 1 cut(s) 685
Alw26I GTCTC 2 cut(s) 354, 679
AlwI GGATC 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 159
AoxI GGCC 2 cut(s) 683, 708
ApeKI GCWGC 4 cut(s) 111, 114, 117, 120
ApoI RAATTY 2 cut(s) 591, 619
AspS9I GGNCC 3 cut(s) 243, 656, 683
AsuHPI GGTGA 1 cut(s) 723
AvaI CYCGRG 1 cut(s) 159
AvaII GGWCC 2 cut(s) 243, 656
BalI TGGCCA 1 cut(s) 710
BbsI GAAGAC 1 cut(s) 704
BbvI GCAGC 4 cut(s) 123, 126, 129, 132
BccI CCATC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 687
BcoDI GTCTC 2 cut(s) 354, 679
BfaI CTAG 2 cut(s) 207, 653
BfmI CTRYAG 1 cut(s) 678
BfuAI ACCTGC 2 cut(s) 742, 751
BisI GCNGC 5 cut(s) 109, 112, 115, 118, 121
BlsI GCNGC 5 cut(s) 110, 113, 116, 119, 122
Bme1390I CCNGG 1 cut(s) 687
Bme18I GGWCC 2 cut(s) 243, 656
BmeT110I CYCGRG 1 cut(s) 159
BmgT120I GGNCC 3 cut(s) 243, 656, 683
BmiI GGNNCC 1 cut(s) 245
BmrFI CCNGG 1 cut(s) 687
BmrI ACTGGG 2 cut(s) 170, 228
BmsI GCATC 3 cut(s) 256, 369, 551
BmuI ACTGGG 2 cut(s) 170, 228
BpiI GAAGAC 1 cut(s) 704
BpuEI CTTGAG 2 cut(s) 368, 571
BsaJI CCNNGG 2 cut(s) 414, 626
BsaXI ACNNNNNCTCC 2 cut(s) 520, 550
Bsc4I CCNNNNNNNGG 1 cut(s) 240
Bse1I ACTGG 3 cut(s) 176, 234, 298
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 1 cut(s) 687
BseDI CCNNGG 2 cut(s) 414, 626
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 1 cut(s) 240
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 1 cut(s) 705
BseNI ACTGG 3 cut(s) 176, 234, 298
BseRI GAGGAG 3 cut(s) 36, 61, 541
BseXI GCAGC 4 cut(s) 123, 126, 129, 132
BshFI GGCC 2 cut(s) 685, 710
BsiHKCI CYCGRG 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 240
BsmAI GTCTC 2 cut(s) 354, 679
BsmI GAATGC 1 cut(s) 187
BsnI GGCC 2 cut(s) 685, 710
BsoBI CYCGRG 1 cut(s) 159
Bsp143I GATC 2 cut(s) 305, 571
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 1 cut(s) 109
BspANI GGCC 2 cut(s) 685, 710
BspCNI CTCAG 1 cut(s) 706
BspLI GGNNCC 1 cut(s) 245
BspMI ACCTGC 2 cut(s) 742, 751
BspPI GGATC 1 cut(s) 566
BsrDI GCAATG 1 cut(s) 669
BsrI ACTGG 3 cut(s) 176, 234, 298
BssECI CCNNGG 2 cut(s) 414, 626
BssMI GATC 2 cut(s) 305, 571
BssT1I CCWWGG 1 cut(s) 414
Bst2UI CCWGG 1 cut(s) 687
Bst4CI ACNGT 1 cut(s) 567
Bst6I CTCTTC 1 cut(s) 91
BstC8I GCNNGC 4 cut(s) 125, 129, 316, 320
BstDEI CTNAG 2 cut(s) 94, 714
BstDSI CCRYGG 2 cut(s) 414, 626
BstEII GGTNACC 1 cut(s) 729
BstF5I GGATG 1 cut(s) 271
BstKTI GATC 2 cut(s) 308, 574
BstMAI GTCTC 2 cut(s) 354, 679
BstMBI GATC 2 cut(s) 305, 571
BstMWI GCNNNNNNNGC 4 cut(s) 114, 117, 120, 743
BstNI CCWGG 1 cut(s) 687
BstPI GGTNACC 1 cut(s) 729
BstSCI CCNGG 1 cut(s) 685
BstSFI CTRYAG 1 cut(s) 678
BstV1I GCAGC 4 cut(s) 123, 126, 129, 132
BstV2I GAAGAC 1 cut(s) 704
BsuRI GGCC 2 cut(s) 685, 710
BtgI CCRYGG 2 cut(s) 414, 626
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 183, 711
BveI ACCTGC 2 cut(s) 742, 751
Cac8I GCNNGC 4 cut(s) 125, 129, 316, 320
Cfr13I GGNCC 3 cut(s) 243, 656, 683
Csp6I GTAC 1 cut(s) 443
CviAII CATG 1 cut(s) 415
CviJI RGCY 5 cut(s) 108, 405, 631, 685, 710
CviKI_1 RGCY 5 cut(s) 108, 405, 631, 685, 710
CviQI GTAC 1 cut(s) 443
DdeI CTNAG 2 cut(s) 94, 714
DpnI GATC 2 cut(s) 307, 573
DpnII GATC 2 cut(s) 305, 571
EaeI YGGCCR 1 cut(s) 708
Eam1104I CTCTTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 414
Eco47I GGWCC 2 cut(s) 243, 656
Eco88I CYCGRG 1 cut(s) 159
Eco91I GGTNACC 1 cut(s) 729
EcoO109I RGGNCCY 2 cut(s) 243, 683
EcoO65I GGTNACC 1 cut(s) 729
EcoRI GAATTC 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 685
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
FaeI CATG 1 cut(s) 418
FaiI YATR 9 cut(s) 41, 105, 416, 500, 502, 524, 557, 599, 680
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FatI CATG 1 cut(s) 414
FblI GTMKAC 1 cut(s) 646
Fnu4HI GCNGC 5 cut(s) 109, 112, 115, 118, 121
FokI GGATG 1 cut(s) 278
Fsp4HI GCNGC 5 cut(s) 109, 112, 115, 118, 121
FspBI CTAG 2 cut(s) 207, 653
GluI GCNGC 5 cut(s) 109, 112, 115, 118, 121
HaeIII GGCC 2 cut(s) 685, 710
Hin1II CATG 1 cut(s) 418
HincII GTYRAC 2 cut(s) 421, 647
HindII GTYRAC 2 cut(s) 421, 647
HinfI GANTC 1 cut(s) 203
HphI GGTGA 1 cut(s) 723
Hpy166II GTNNAC 3 cut(s) 259, 421, 647
Hpy188III TCNNGA 2 cut(s) 588, 615
Hpy8I GTNNAC 3 cut(s) 259, 421, 647
HpyAV CCTTC 3 cut(s) 76, 291, 585
HpyCH4III ACNGT 1 cut(s) 567
HpyCH4V TGCA 1 cut(s) 318
HpyF10VI GCNNNNNNNGC 4 cut(s) 114, 117, 120, 743
HpyF3I CTNAG 2 cut(s) 94, 714
Hsp92II CATG 1 cut(s) 418
Kzo9I GATC 2 cut(s) 305, 571
LmnI GCTCC 1 cut(s) 212
Lsp1109I GCAGC 4 cut(s) 123, 126, 129, 132
LweI GCATC 3 cut(s) 256, 369, 551
MaeI CTAG 2 cut(s) 207, 653
MaeIII GTNAC 2 cut(s) 488, 729
MalI GATC 2 cut(s) 307, 573
MboI GATC 2 cut(s) 305, 571
MboII GAAGA 4 cut(s) 78, 293, 484, 709
MlsI TGGCCA 1 cut(s) 710
MluCI AATT 9 cut(s) 333, 396, 424, 447, 477, 509, 591, 619, 757
MluNI TGGCCA 1 cut(s) 710
MnlI CCTC 9 cut(s) 54, 57, 82, 142, 155, 184, 234, 257, 519
Mox20I TGGCCA 1 cut(s) 710
MscI TGGCCA 1 cut(s) 710
MseI TTAA 2 cut(s) 395, 669
Msp20I TGGCCA 1 cut(s) 710
MspR9I CCNGG 1 cut(s) 687
Mva1269I GAATGC 1 cut(s) 187
MvaI CCWGG 1 cut(s) 687
MwoI GCNNNNNNNGC 4 cut(s) 114, 117, 120, 743
NcoI CCATGG 1 cut(s) 414
NdeII GATC 2 cut(s) 305, 571
NlaIII CATG 1 cut(s) 418
NlaIV GGNNCC 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 729
PaeR7I CTCGAG 1 cut(s) 159
PaqCI CACCTGC 2 cut(s) 742, 751
PctI GAATGC 1 cut(s) 187
PfeI GAWTC 1 cut(s) 203
PkrI GCNGC 5 cut(s) 110, 113, 116, 119, 122
PpuMI RGGWCCY 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 243
Psp6I CCWGG 1 cut(s) 685
PspEI GGTNACC 1 cut(s) 729
PspGI CCWGG 1 cut(s) 685
PspN4I GGNNCC 1 cut(s) 245
PspPI GGNCC 3 cut(s) 243, 656, 683
PspPPI RGGWCCY 1 cut(s) 243
PspXI VCTCGAGB 1 cut(s) 159
RsaI GTAC 1 cut(s) 444
RsaNI GTAC 1 cut(s) 443
SalI GTCGAC 1 cut(s) 645
SaqAI TTAA 2 cut(s) 395, 669
SatI GCNGC 5 cut(s) 109, 112, 115, 118, 121
Sau3AI GATC 2 cut(s) 305, 571
Sau96I GGNCC 3 cut(s) 243, 656, 683
ScrFI CCNGG 1 cut(s) 687
SetI ASST 5 cut(s) 46, 166, 457, 736, 745
SfaNI GCATC 3 cut(s) 256, 369, 551
SfcI CTRYAG 1 cut(s) 678
Sfr274I CTCGAG 1 cut(s) 159
SinI GGWCC 2 cut(s) 243, 656
SlaI CTCGAG 1 cut(s) 159
SmlI CTYRAG 3 cut(s) 159, 383, 586
SmoI CTYRAG 3 cut(s) 159, 383, 586
Sse9I AATT 9 cut(s) 333, 396, 424, 447, 477, 509, 591, 619, 757
SsiI CCGC 1 cut(s) 109
SspMI CTAG 2 cut(s) 207, 653
StyD4I CCNGG 1 cut(s) 685
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 1 cut(s) 567
TaqI TCGA 3 cut(s) 74, 160, 646
TasI AATT 9 cut(s) 333, 396, 424, 447, 477, 509, 591, 619, 757
TatI WGTACW 1 cut(s) 442
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 1 cut(s) 203
Tru1I TTAA 2 cut(s) 395, 669
Tru9I TTAA 2 cut(s) 395, 669
TscAI CASTG 2 cut(s) 183, 718
TseFI GTSAC 1 cut(s) 729
TseI GCWGC 4 cut(s) 111, 114, 117, 120
Tsp45I GTSAC 1 cut(s) 729
TspDTI ATGAA 2 cut(s) 294, 300
TspGWI ACGGA 1 cut(s) 615
TspRI CASTG 2 cut(s) 183, 718
VpaK11BI GGWCC 2 cut(s) 243, 656
XapI RAATTY 2 cut(s) 591, 619
XhoI CTCGAG 1 cut(s) 159
XmiI GTMKAC 1 cut(s) 646
XspI CTAG 2 cut(s) 207, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.