pycom08g14880

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001161
Physical Location & Seq
Forward (+)
14292940 .. 14295450
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g14880.1

Sequence Viewer

Length: 975 bp
ATGGCGAATTCGTTGTTTCTCCCAAATCACACTTTGATAGCTCCTCCTCCTCGTCGCCTCTCCGCCTCCGTCTCCTCGAAACCCAACAAACCCCAACCCCCTCTTCTTCCTTGTCCTCCTCCTCCTCCTCCTCTAGGGTTTATCAACTTCTCTCTCACCTCCAACTCTTCCTCCTCCATTATTAATCCCAGCAGCGGCGGCACCAGCAGCAGCAGGAGGCGAGCCGCCTCTTCCTTCAAGTCTCGTCTTGCTCCTCAGGACTCGGTTCCCCCAACGAATTCCAGAAAGGAAAGTAGTAAGAGCAATGCGGGACCTTCTGGAAGCTGGGGAGATGAAGAGCAGTTGCCCTCTTTGAAAACCCTCCTTAAAGTTTACAAGGAAGCTATTTTTAACGGGGATGGAAAAACTGTATCCGAAGTTGAGGCTATTATACGAGTGATAGAAAATGAGAAGAAAGAGTTAGTGGAGAAAGTATCTTCTGCATCAGCAGAGATAACGTCTGGGAAGGAGAAATTGATCCGCTTGCAAGCAGATTTTGATAATTGTAGAAAAAGATTCGAGAAGGAGAGGCTGACCGTAAGGACGGACGCCCAAGGGGAAGTGATTGAGAGTCTTTTGCCCATGGTTGACAATTTTGAGAGAGCCAGGCAATACCTTAAACCCGAGACTGAAAAGGAAAAAAAGATTGATGCAAGTTACCAAGGTATATACAAGCAATTCGTTGAGATTATGAGGAGCTTGCATGTGGCCGTCGTTCCAACCGTGGGAAAGCCTTTCGATCCTTCGTTGCATGAAGCTATTGCACGCGAAGAGTCTCAAGAGTTAAAGGAAGGGATTATAATCCAAGAAATCCGCCGCGGGTTTTTACTCGGAGGTCGGCTTCTGAGACCAGCTACGGTTAAAGTCTCATCAGGGCCCGGCAGTAAGAAATCCCCAGTCGCCACTGAGAAAACAACTGCAGGAGTGGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

325

Amino Acids

35.45

Weight (kDa)

9.55

Isoelectric Point (pI)

54.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 148 - 303 2.8e-39 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 839
AccB1I GGYRCC 1 cut(s) 200
AccII CGCG 2 cut(s) 807, 858
AciI CCGC 9 cut(s) 63, 195, 198, 225, 308, 520, 853, 856, 858
AclWI GGATC 2 cut(s) 511, 773
AcoI YGGCCR 1 cut(s) 747
AcsI RAATTY 2 cut(s) 7, 277
AcyI GRCGYC 1 cut(s) 588
AfiI CCNNNNNNNGG 3 cut(s) 134, 194, 764
AgsI TTSAA 2 cut(s) 238, 355
AjnI CCWGG 1 cut(s) 644
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AluBI AGCT 6 cut(s) 41, 324, 383, 738, 797, 893
AluI AGCT 6 cut(s) 41, 324, 383, 738, 797, 893
Alw26I GTCTC 6 cut(s) 76, 246, 659, 819, 880, 910
AlwI GGATC 2 cut(s) 511, 773
Ama87I CYCGRG 1 cut(s) 662
AoxI GGCC 2 cut(s) 747, 914
ApaI GGGCCC 1 cut(s) 918
ApeKI GCWGC 3 cut(s) 192, 207, 210
ApoI RAATTY 2 cut(s) 7, 277
AseI ATTAAT 1 cut(s) 183
AspS9I GGNCC 3 cut(s) 311, 914, 915
AsuC2I CCSGG 1 cut(s) 918
AsuHPI GGTGA 1 cut(s) 148
AvaI CYCGRG 1 cut(s) 662
AvaII GGWCC 1 cut(s) 311
AxyI CCTNAGG 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 918
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 1 cut(s) 918
BbvI GCAGC 3 cut(s) 204, 219, 222
BccI CCATC 1 cut(s) 392
BceAI ACGGC 1 cut(s) 734
BciT130I CCWGG 1 cut(s) 646
BciVI GTATCC 1 cut(s) 421
BcnI CCSGG 1 cut(s) 918
BcoDI GTCTC 6 cut(s) 76, 246, 659, 819, 880, 910
BfaI CTAG 1 cut(s) 134
BfmI CTRYAG 1 cut(s) 957
BfuI GTATCC 1 cut(s) 421
BisI GCNGC 7 cut(s) 193, 196, 199, 208, 211, 225, 856
BlsI GCNGC 7 cut(s) 194, 197, 200, 209, 212, 226, 857
Bme1390I CCNGG 2 cut(s) 646, 918
Bme18I GGWCC 1 cut(s) 311
BmeT110I CYCGRG 1 cut(s) 662
BmgT120I GGNCC 3 cut(s) 311, 914, 915
BmiI GGNNCC 4 cut(s) 202, 267, 312, 916
BmrFI CCNGG 2 cut(s) 646, 918
BmrI ACTGGG 1 cut(s) 929
BmsI GCATC 2 cut(s) 491, 679
BmuI ACTGGG 1 cut(s) 929
BpuEI CTTGAG 1 cut(s) 801
BpuMI CCSGG 1 cut(s) 918
BsaBI GATNNNNATC 1 cut(s) 839
BsaHI GRCGYC 1 cut(s) 588
BsaI GGTCTC 1 cut(s) 880
BsaJI CCNNGG 5 cut(s) 592, 621, 700, 762, 856
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 194, 764
Bse1I ACTGG 1 cut(s) 935
Bse21I CCTNAGG 1 cut(s) 255
Bse3DI GCAATG 1 cut(s) 310
Bse8I GATNNNNATC 1 cut(s) 839
BseBI CCWGG 1 cut(s) 646
BseDI CCNNGG 5 cut(s) 592, 621, 700, 762, 856
BseGI GGATG 1 cut(s) 403
BseJI GATNNNNATC 1 cut(s) 839
BseLI CCNNNNNNNGG 3 cut(s) 134, 194, 764
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 3 cut(s) 269, 875, 936
BseNI ACTGG 1 cut(s) 935
BseSI GKGCMC 1 cut(s) 918
BseXI GCAGC 3 cut(s) 204, 219, 222
BseYI CCCAGC 2 cut(s) 188, 324
Bsh1236I CGCG 2 cut(s) 807, 858
BshFI GGCC 2 cut(s) 749, 916
BshNI GGYRCC 1 cut(s) 200
BsiHKCI CYCGRG 1 cut(s) 662
BsiSI CCGG 1 cut(s) 918
BslFI GGGAC 1 cut(s) 324
BslI CCNNNNNNNGG 3 cut(s) 134, 194, 764
BsmAI GTCTC 6 cut(s) 76, 246, 659, 819, 880, 910
BsmBI CGTCTC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 324
BsnI GGCC 2 cut(s) 749, 916
Bso31I GGTCTC 1 cut(s) 880
BsoBI CYCGRG 1 cut(s) 662
Bsp120I GGGCCC 1 cut(s) 914
Bsp1286I GDGCHC 1 cut(s) 918
Bsp143I GATC 2 cut(s) 516, 778
Bsp19I CCATGG 1 cut(s) 621
BspACI CCGC 9 cut(s) 63, 195, 198, 225, 308, 520, 853, 856, 858
BspANI GGCC 2 cut(s) 749, 916
BspCNI CTCAG 3 cut(s) 268, 876, 937
BspFNI CGCG 2 cut(s) 807, 858
BspLI GGNNCC 4 cut(s) 202, 267, 312, 916
BspMAI CTGCAG 1 cut(s) 961
BspPI GGATC 2 cut(s) 511, 773
BspQI GCTCTTC 1 cut(s) 330
BspT107I GGYRCC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 880
BsrDI GCAATG 1 cut(s) 310
BsrI ACTGG 1 cut(s) 935
BssECI CCNNGG 5 cut(s) 592, 621, 700, 762, 856
BssMI GATC 2 cut(s) 516, 778
BssNI GRCGYC 1 cut(s) 588
BssT1I CCWWGG 3 cut(s) 592, 621, 700
Bst2UI CCWGG 1 cut(s) 646
Bst4CI ACNGT 4 cut(s) 409, 577, 763, 898
Bst6I CTCTTC 5 cut(s) 108, 172, 235, 330, 804
BstACI GRCGYC 1 cut(s) 588
BstC8I GCNNGC 5 cut(s) 222, 524, 528, 740, 805
BstDEI CTNAG 3 cut(s) 255, 884, 945
BstDSI CCRYGG 3 cut(s) 621, 762, 856
BstENI CCTNNNNNAGG 1 cut(s) 132
BstF5I GGATG 1 cut(s) 403
BstFNI CGCG 2 cut(s) 807, 858
BstKTI GATC 2 cut(s) 519, 781
BstMAI GTCTC 6 cut(s) 76, 246, 659, 819, 880, 910
BstMBI GATC 2 cut(s) 516, 778
BstMWI GCNNNNNNNGC 3 cut(s) 198, 204, 207
BstNI CCWGG 1 cut(s) 646
BstNSI RCATGY 1 cut(s) 746
BstSCI CCNGG 2 cut(s) 644, 916
BstSFI CTRYAG 1 cut(s) 957
BstSLI GKGCMC 1 cut(s) 918
BstUI CGCG 2 cut(s) 807, 858
BstV1I GCAGC 3 cut(s) 204, 219, 222
Bsu36I CCTNAGG 1 cut(s) 255
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 2 cut(s) 749, 916
BtgI CCRYGG 3 cut(s) 621, 762, 856
BtsCI GGATG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 942
Cac8I GCNNGC 5 cut(s) 222, 524, 528, 740, 805
Cfr13I GGNCC 3 cut(s) 311, 914, 915
Cfr42I CCGCGG 1 cut(s) 859
CseI GACGC 1 cut(s) 596
CviAII CATG 3 cut(s) 622, 743, 791
DdeI CTNAG 3 cut(s) 255, 884, 945
DpnI GATC 2 cut(s) 518, 780
DpnII GATC 2 cut(s) 516, 778
EaeI YGGCCR 1 cut(s) 747
Eam1104I CTCTTC 5 cut(s) 108, 172, 235, 330, 804
EarI CTCTTC 5 cut(s) 108, 172, 235, 330, 804
EciI GGCGGA 2 cut(s) 52, 842
Eco130I CCWWGG 3 cut(s) 592, 621, 700
Eco24I GRGCYC 1 cut(s) 918
Eco31I GGTCTC 1 cut(s) 880
Eco47I GGWCC 1 cut(s) 311
Eco81I CCTNAGG 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 662
EcoNI CCTNNNNNAGG 1 cut(s) 132
EcoO109I RGGNCCY 2 cut(s) 311, 914
EcoRI GAATTC 2 cut(s) 7, 277
EcoRII CCWGG 1 cut(s) 644
EcoT14I CCWWGG 3 cut(s) 592, 621, 700
EcoT38I GRGCYC 1 cut(s) 918
ErhI CCWWGG 3 cut(s) 592, 621, 700
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 3 cut(s) 625, 746, 794
FaiI YATR 8 cut(s) 431, 623, 707, 709, 731, 744, 792, 839
FaqI GGGAC 1 cut(s) 324
FatI CATG 3 cut(s) 621, 742, 790
FauI CCCGC 2 cut(s) 301, 851
Fnu4HI GCNGC 7 cut(s) 193, 196, 199, 208, 211, 225, 856
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 1 cut(s) 918
Fsp4HI GCNGC 7 cut(s) 193, 196, 199, 208, 211, 225, 856
FspBI CTAG 1 cut(s) 134
GluI GCNGC 7 cut(s) 193, 196, 199, 208, 211, 225, 856
GsaI CCCAGC 2 cut(s) 192, 328
HaeIII GGCC 2 cut(s) 749, 916
HapII CCGG 1 cut(s) 918
HgaI GACGC 1 cut(s) 596
Hin1I GRCGYC 1 cut(s) 588
Hin1II CATG 3 cut(s) 625, 746, 794
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HinfI GANTC 4 cut(s) 260, 555, 610, 812
HpaII CCGG 1 cut(s) 918
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 2 cut(s) 373, 628
Hpy188I TCNGA 3 cut(s) 415, 872, 885
Hpy188III TCNNGA 5 cut(s) 257, 282, 318, 559, 818
Hpy8I GTNNAC 2 cut(s) 373, 628
Hpy99I CGWCG 2 cut(s) 57, 755
HpyAV CCTTC 6 cut(s) 244, 324, 499, 556, 792, 824
HpyCH4III ACNGT 4 cut(s) 409, 577, 763, 898
HpyCH4IV ACGT 1 cut(s) 497
HpyCH4V TGCA 7 cut(s) 482, 526, 692, 742, 790, 803, 959
HpyF10VI GCNNNNNNNGC 3 cut(s) 198, 204, 207
HpyF3I CTNAG 3 cut(s) 255, 884, 945
HpySE526I ACGT 1 cut(s) 497
Hsp92I GRCGYC 1 cut(s) 588
Hsp92II CATG 3 cut(s) 625, 746, 794
KspI CCGCGG 1 cut(s) 859
Kzo9I GATC 2 cut(s) 516, 778
LguI GCTCTTC 1 cut(s) 330
LmnI GCTCC 3 cut(s) 46, 256, 735
Lsp1109I GCAGC 3 cut(s) 204, 219, 222
LweI GCATC 2 cut(s) 491, 679
MaeI CTAG 1 cut(s) 134
MaeII ACGT 1 cut(s) 497
MaeIII GTNAC 1 cut(s) 695
MalI GATC 2 cut(s) 518, 780
MboI GATC 2 cut(s) 516, 778
MboII GAAGA 8 cut(s) 95, 98, 159, 222, 347, 463, 468, 821
MhlI GDGCHC 1 cut(s) 918
MluCI AATT 6 cut(s) 7, 277, 512, 541, 631, 716
MlyI GAGTC 3 cut(s) 254, 619, 821
MmeI TCCRAC 2 cut(s) 186, 782
MseI TTAA 6 cut(s) 183, 366, 390, 657, 824, 900
MspA1I CMGCKG 2 cut(s) 195, 858
MspI CCGG 1 cut(s) 918
MspR9I CCNGG 2 cut(s) 646, 918
MvaI CCWGG 1 cut(s) 646
MvnI CGCG 2 cut(s) 807, 858
MwoI GCNNNNNNNGC 3 cut(s) 198, 204, 207
NciI CCSGG 1 cut(s) 918
NcoI CCATGG 1 cut(s) 621
NdeII GATC 2 cut(s) 516, 778
NlaIII CATG 3 cut(s) 625, 746, 794
NlaIV GGNNCC 4 cut(s) 202, 267, 312, 916
NspI RCATGY 1 cut(s) 746
PciSI GCTCTTC 1 cut(s) 330
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PfeI GAWTC 1 cut(s) 555
PkrI GCNGC 7 cut(s) 194, 197, 200, 209, 212, 226, 857
PleI GAGTC 3 cut(s) 254, 618, 820
PpsI GAGTC 3 cut(s) 254, 618, 820
PpuMI RGGWCCY 1 cut(s) 311
PshBI ATTAAT 1 cut(s) 183
PsiI TTATAA 1 cut(s) 839
Psp5II RGGWCCY 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 644
PspFI CCCAGC 2 cut(s) 188, 324
PspGI CCWGG 1 cut(s) 644
PspN4I GGNNCC 4 cut(s) 202, 267, 312, 916
PspOMI GGGCCC 1 cut(s) 914
PspPI GGNCC 3 cut(s) 311, 914, 915
PspPPI RGGWCCY 1 cut(s) 311
PstI CTGCAG 1 cut(s) 961
SacII CCGCGG 1 cut(s) 859
SapI GCTCTTC 1 cut(s) 330
SaqAI TTAA 6 cut(s) 183, 366, 390, 657, 824, 900
SatI GCNGC 7 cut(s) 193, 196, 199, 208, 211, 225, 856
Sau3AI GATC 2 cut(s) 516, 778
Sau96I GGNCC 3 cut(s) 311, 914, 915
SchI GAGTC 3 cut(s) 254, 619, 821
ScrFI CCNGG 2 cut(s) 646, 918
SduI GDGCHC 1 cut(s) 918
SfaNI GCATC 2 cut(s) 491, 679
SfcI CTRYAG 1 cut(s) 957
Sfr303I CCGCGG 1 cut(s) 859
SgrBI CCGCGG 1 cut(s) 859
SinI GGWCC 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 816
SmoI CTYRAG 1 cut(s) 816
Sse9I AATT 6 cut(s) 7, 277, 512, 541, 631, 716
SsiI CCGC 9 cut(s) 63, 195, 198, 225, 308, 520, 853, 856, 858
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 2 cut(s) 644, 916
StyI CCWWGG 3 cut(s) 592, 621, 700
TaaI ACNGT 4 cut(s) 409, 577, 763, 898
TaiI ACGT 1 cut(s) 500
TaqI TCGA 3 cut(s) 77, 558, 777
TasI AATT 6 cut(s) 7, 277, 512, 541, 631, 716
TauI GCSGC 4 cut(s) 198, 201, 227, 858
TfiI GAWTC 1 cut(s) 555
Tru1I TTAA 6 cut(s) 183, 366, 390, 657, 824, 900
Tru9I TTAA 6 cut(s) 183, 366, 390, 657, 824, 900
TscAI CASTG 1 cut(s) 949
TseI GCWGC 3 cut(s) 192, 207, 210
TspDTI ATGAA 2 cut(s) 348, 807
TspGWI ACGGA 2 cut(s) 58, 599
TspRI CASTG 1 cut(s) 949
VpaK11BI GGWCC 1 cut(s) 311
VspI ATTAAT 1 cut(s) 183
XagI CCTNNNNNAGG 1 cut(s) 132
XapI RAATTY 2 cut(s) 7, 277
XceI RCATGY 1 cut(s) 746
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.