Rw7G041170

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001161
Physical Location & Seq
Reverse (-)
64111677 .. 64114620
2944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041170.1

Sequence Viewer

Length: 882 bp
ATGACGAACTTGTTGTTTATCCCAAACAACTACTTCGTCATACCTCCTCCTCGCGTTTCTGCTTCCTCCTTCTCCTCAAAACCCATCGCTCCTCAACCTCTTCTAGCTTTTCACCGCAGCAGTAGCAGGCGCGCCTTGTTTTTCAAATCTCATCTTTCTGCTCGAGGCTCTGTTCCCCCAGTGAATGCAAAAGAGGATAATGTAGATTCTAGTGGAGCAGATCAACAACATTTGCCTAGTTTGAGGACCCTCCTTAAAGTTTACAAGGATGCTATTTTCAATGGAGATGAAAAGACCGTATCCGAAATTGAGGCAAGGATAGAAGTAATAGAAAATAAGCAGAATGAATTAGTCCAGAAGGTATCATCTATATCTGCAGAGGTAACATCAGGGAAGGAAAAGCTTATCCGCTTGCAAGCAGATTTTGATAATTGTAGAAAAAGATTTGAGAAAGAGAGACTTACCGTGAGGACTGATGCTCAAGGAGAAGTAATTGAGAGCCTTTTGCCCATGGTTGACAATTTTGAGAGAGCAAAACAACAAATTAGACCTGAAACTGAAAAGGAGAAGAAGATTGATACAAGTTACCAAGGTATATACAAGCAATTTGTTGAGATTATGAGGAGCTTGCGTGTAGCCTCTGTGCCAACTAAGGGAAAACCCTTTGATCCTTCGGTGCATGAAGCTATTGCAAGAGAAGAGTCTCAAGAATTTCCTGAGGGGATTGTCATTCAAGAGATTCGCCGTGGCTTTTTACTTGGAGGTAGACTTCTAAGACCAGCAATGGTTAAAGTCTCTATAGGGCCTGGCAGAAAGAAATCCCCTGTGGCCACTGAGAAATCATCAGGGTCACCTGCAACAGCCGCTTCGGTAGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

293

Amino Acids

32.69

Weight (kDa)

9.47

Isoelectric Point (pI)

53.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 110 - 266 6.1e-40 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 862
Acc36I ACCTGC 1 cut(s) 862
AccI GTMKAC 1 cut(s) 766
AccII CGCG 2 cut(s) 54, 132
AciI CCGC 3 cut(s) 115, 409, 864
AclWI GGATC 1 cut(s) 662
AcoI YGGCCR 1 cut(s) 828
AcsI RAATTY 1 cut(s) 710
AfiI CCNNNNNNNGG 1 cut(s) 653
AgsI TTSAA 3 cut(s) 145, 280, 734
AjnI CCWGG 1 cut(s) 805
AluBI AGCT 4 cut(s) 107, 403, 627, 686
AluI AGCT 4 cut(s) 107, 403, 627, 686
Alw26I GTCTC 3 cut(s) 451, 708, 799
AlwI GGATC 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 162
AoxI GGCC 2 cut(s) 803, 828
ApeKI GCWGC 1 cut(s) 117
ApoI RAATTY 1 cut(s) 710
AscI GGCGCGCC 1 cut(s) 130
AspLEI GCGC 2 cut(s) 132, 134
AspS9I GGNCC 2 cut(s) 246, 803
AsuHPI GGTGA 2 cut(s) 104, 843
AvaI CYCGRG 1 cut(s) 162
AvaII GGWCC 1 cut(s) 246
AxyI CCTNAGG 1 cut(s) 717
BalI TGGCCA 1 cut(s) 830
BbvI GCAGC 1 cut(s) 129
BccI CCATC 1 cut(s) 92
BceAI ACGGC 1 cut(s) 729
BciT130I CCWGG 1 cut(s) 807
BciVI GTATCC 1 cut(s) 310
BcoDI GTCTC 3 cut(s) 451, 708, 799
BfaI CTAG 3 cut(s) 104, 210, 237
BfmI CTRYAG 2 cut(s) 375, 798
BfuAI ACCTGC 1 cut(s) 862
BfuI GTATCC 1 cut(s) 310
BisI GCNGC 2 cut(s) 118, 864
BlsI GCNGC 2 cut(s) 119, 865
Bme1390I CCNGG 1 cut(s) 807
Bme18I GGWCC 1 cut(s) 246
BmeT110I CYCGRG 1 cut(s) 162
BmgT120I GGNCC 2 cut(s) 246, 803
BmiI GGNNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 807
BmrI ACTGGG 1 cut(s) 173
BmsI GCATC 2 cut(s) 259, 466
BmuI ACTGGG 1 cut(s) 173
BpuEI CTTGAG 2 cut(s) 465, 690
BsaJI CCNNGG 3 cut(s) 510, 589, 745
BsaXI ACNNNNNCTCC 2 cut(s) 616, 646
Bsc4I CCNNNNNNNGG 1 cut(s) 653
Bse1I ACTGG 1 cut(s) 179
Bse21I CCTNAGG 1 cut(s) 717
Bse3DI GCAATG 1 cut(s) 789
BseBI CCWGG 1 cut(s) 807
BseDI CCNNGG 3 cut(s) 510, 589, 745
BseGI GGATG 1 cut(s) 274
BseLI CCNNNNNNNGG 1 cut(s) 653
BseMI GCAATG 1 cut(s) 789
BseMII CTCAG 2 cut(s) 708, 825
BseNI ACTGG 1 cut(s) 179
BsePI GCGCGC 1 cut(s) 130
BseRI GAGGAG 5 cut(s) 36, 39, 64, 81, 637
BseXI GCAGC 1 cut(s) 129
Bsh1236I CGCG 2 cut(s) 54, 132
BshFI GGCC 2 cut(s) 805, 830
BsiHKCI CYCGRG 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 653
BsmAI GTCTC 3 cut(s) 451, 708, 799
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 2 cut(s) 805, 830
BsoBI CYCGRG 1 cut(s) 162
Bsp143I GATC 2 cut(s) 220, 667
Bsp19I CCATGG 1 cut(s) 510
BspACI CCGC 3 cut(s) 115, 409, 864
BspANI GGCC 2 cut(s) 805, 830
BspCNI CTCAG 2 cut(s) 709, 826
BspFNI CGCG 2 cut(s) 54, 132
BspLI GGNNCC 1 cut(s) 248
BspMAI CTGCAG 1 cut(s) 379
BspMI ACCTGC 1 cut(s) 862
BspPI GGATC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 789
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 3 cut(s) 510, 589, 745
BssHII GCGCGC 1 cut(s) 130
BssMI GATC 2 cut(s) 220, 667
BssT1I CCWWGG 2 cut(s) 510, 589
Bst2UI CCWGG 1 cut(s) 807
Bst4CI ACNGT 2 cut(s) 298, 466
Bst6I CTCTTC 2 cut(s) 105, 693
BstC8I GCNNGC 5 cut(s) 128, 132, 413, 417, 629
BstDEI CTNAG 4 cut(s) 651, 717, 773, 834
BstDSI CCRYGG 2 cut(s) 510, 745
BstEII GGTNACC 1 cut(s) 849
BstF5I GGATG 1 cut(s) 274
BstFNI CGCG 2 cut(s) 54, 132
BstHHI GCGC 2 cut(s) 132, 134
BstKTI GATC 2 cut(s) 223, 670
BstMAI GTCTC 3 cut(s) 451, 708, 799
BstMBI GATC 2 cut(s) 220, 667
BstMWI GCNNNNNNNGC 2 cut(s) 123, 863
BstNI CCWGG 1 cut(s) 807
BstPI GGTNACC 1 cut(s) 849
BstSCI CCNGG 1 cut(s) 805
BstSFI CTRYAG 2 cut(s) 375, 798
BstUI CGCG 2 cut(s) 54, 132
BstV1I GCAGC 1 cut(s) 129
Bsu36I CCTNAGG 1 cut(s) 717
BsuI GTATCC 1 cut(s) 310
BsuRI GGCC 2 cut(s) 805, 830
BtgI CCRYGG 2 cut(s) 510, 745
BtgZI GCGATG 1 cut(s) 70
BtsCI GGATG 1 cut(s) 274
BtsIMutI CAGTG 2 cut(s) 186, 831
BveI ACCTGC 1 cut(s) 862
Cac8I GCNNGC 5 cut(s) 128, 132, 413, 417, 629
CfoI GCGC 2 cut(s) 132, 134
Cfr13I GGNCC 2 cut(s) 246, 803
CviAII CATG 2 cut(s) 511, 680
DdeI CTNAG 4 cut(s) 651, 717, 773, 834
DpnI GATC 2 cut(s) 222, 669
DpnII GATC 2 cut(s) 220, 667
EaeI YGGCCR 1 cut(s) 828
Eam1104I CTCTTC 2 cut(s) 105, 693
EarI CTCTTC 2 cut(s) 105, 693
Eco130I CCWWGG 2 cut(s) 510, 589
Eco47I GGWCC 1 cut(s) 246
Eco81I CCTNAGG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 162
Eco91I GGTNACC 1 cut(s) 849
EcoO109I RGGNCCY 2 cut(s) 246, 803
EcoO65I GGTNACC 1 cut(s) 849
EcoRII CCWGG 1 cut(s) 805
EcoT14I CCWWGG 2 cut(s) 510, 589
ErhI CCWWGG 2 cut(s) 510, 589
FaeI CATG 2 cut(s) 514, 683
FaiI YATR 8 cut(s) 41, 371, 512, 596, 598, 620, 681, 800
FalI AAGNNNNNCTT 2 cut(s) 444, 476
FatI CATG 2 cut(s) 510, 679
FblI GTMKAC 1 cut(s) 766
Fnu4HI GCNGC 2 cut(s) 118, 864
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 2 cut(s) 118, 864
FspBI CTAG 3 cut(s) 104, 210, 237
GlaI GCGC 2 cut(s) 131, 133
GluI GCNGC 2 cut(s) 118, 864
HaeIII GGCC 2 cut(s) 805, 830
HhaI GCGC 2 cut(s) 132, 134
Hin1II CATG 2 cut(s) 514, 683
Hin6I GCGC 2 cut(s) 130, 132
HinP1I GCGC 2 cut(s) 130, 132
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 3 cut(s) 206, 701, 739
HphI GGTGA 2 cut(s) 104, 843
Hpy166II GTNNAC 3 cut(s) 262, 517, 767
Hpy188I TCNGA 1 cut(s) 304
Hpy188III TCNNGA 4 cut(s) 355, 707, 716, 734
Hpy8I GTNNAC 3 cut(s) 262, 517, 767
HpyAV CCTTC 4 cut(s) 79, 352, 388, 681
HpyCH4III ACNGT 2 cut(s) 298, 466
HpyCH4V TGCA 6 cut(s) 188, 377, 415, 679, 692, 857
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 863
HpyF3I CTNAG 4 cut(s) 651, 717, 773, 834
Hsp92II CATG 2 cut(s) 514, 683
HspAI GCGC 2 cut(s) 130, 132
Kzo9I GATC 2 cut(s) 220, 667
LmnI GCTCC 3 cut(s) 94, 215, 624
Lsp1109I GCAGC 1 cut(s) 129
LweI GCATC 2 cut(s) 259, 466
MaeI CTAG 3 cut(s) 104, 210, 237
MaeIII GTNAC 3 cut(s) 382, 584, 849
MalI GATC 2 cut(s) 222, 669
MboI GATC 2 cut(s) 220, 667
MboII GAAGA 4 cut(s) 92, 580, 583, 710
MlsI TGGCCA 1 cut(s) 830
MluCI AATT 9 cut(s) 306, 347, 430, 492, 520, 543, 605, 710, 877
MluNI TGGCCA 1 cut(s) 830
MlyI GAGTC 1 cut(s) 710
Mox20I TGGCCA 1 cut(s) 830
MscI TGGCCA 1 cut(s) 830
MseI TTAA 2 cut(s) 255, 789
Msp20I TGGCCA 1 cut(s) 830
MspR9I CCNGG 1 cut(s) 807
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 1 cut(s) 807
MvnI CGCG 2 cut(s) 54, 132
MwoI GCNNNNNNNGC 2 cut(s) 123, 863
NcoI CCATGG 1 cut(s) 510
NdeII GATC 2 cut(s) 220, 667
NlaIII CATG 2 cut(s) 514, 683
NlaIV GGNNCC 1 cut(s) 248
NmuCI GTSAC 1 cut(s) 849
PaeR7I CTCGAG 1 cut(s) 162
PalAI GGCGCGCC 1 cut(s) 130
PaqCI CACCTGC 1 cut(s) 862
PauI GCGCGC 1 cut(s) 130
PctI GAATGC 1 cut(s) 190
PfeI GAWTC 2 cut(s) 206, 739
PkrI GCNGC 2 cut(s) 119, 865
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
PpuMI RGGWCCY 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 805
PspEI GGTNACC 1 cut(s) 849
PspGI CCWGG 1 cut(s) 805
PspN4I GGNNCC 1 cut(s) 248
PspPI GGNCC 2 cut(s) 246, 803
PspPPI RGGWCCY 1 cut(s) 246
PspXI VCTCGAGB 1 cut(s) 162
PstI CTGCAG 1 cut(s) 379
PteI GCGCGC 1 cut(s) 130
SaqAI TTAA 2 cut(s) 255, 789
SatI GCNGC 2 cut(s) 118, 864
Sau3AI GATC 2 cut(s) 220, 667
Sau96I GGNCC 2 cut(s) 246, 803
SchI GAGTC 1 cut(s) 710
ScrFI CCNGG 1 cut(s) 807
SfaNI GCATC 2 cut(s) 259, 466
SfcI CTRYAG 2 cut(s) 375, 798
Sfr274I CTCGAG 1 cut(s) 162
SgsI GGCGCGCC 1 cut(s) 130
SinI GGWCC 1 cut(s) 246
SlaI CTCGAG 1 cut(s) 162
SmlI CTYRAG 3 cut(s) 162, 480, 705
SmoI CTYRAG 3 cut(s) 162, 480, 705
Sse9I AATT 9 cut(s) 306, 347, 430, 492, 520, 543, 605, 710, 877
SsiI CCGC 3 cut(s) 115, 409, 864
SspMI CTAG 3 cut(s) 104, 210, 237
StyD4I CCNGG 1 cut(s) 805
StyI CCWWGG 2 cut(s) 510, 589
TaaI ACNGT 2 cut(s) 298, 466
TaqI TCGA 1 cut(s) 163
TasI AATT 9 cut(s) 306, 347, 430, 492, 520, 543, 605, 710, 877
TauI GCSGC 1 cut(s) 866
TfiI GAWTC 2 cut(s) 206, 739
Tru1I TTAA 2 cut(s) 255, 789
Tru9I TTAA 2 cut(s) 255, 789
TscAI CASTG 2 cut(s) 186, 838
TseFI GTSAC 1 cut(s) 849
TseI GCWGC 1 cut(s) 117
Tsp45I GTSAC 1 cut(s) 849
TspDTI ATGAA 3 cut(s) 303, 360, 696
TspRI CASTG 2 cut(s) 186, 838
VpaK11BI GGWCC 1 cut(s) 246
XapI RAATTY 1 cut(s) 710
XhoI CTCGAG 1 cut(s) 162
XmiI GTMKAC 1 cut(s) 766
XspI CTAG 3 cut(s) 104, 210, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.