Rroxscaffold_3G00219900

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001161
Physical Location & Seq
Forward (+)
2101361 .. 2104720
3360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00219900.1

Sequence Viewer

Length: 915 bp
ATGGCGAACTCGTTGTTTATCCCAAACAACTACTTGGTCATACCTCCTCCCGTTTCTGCTTCCTTCTCCTCAAAACCCATTGCTCCTCCTCCTCTACCTCTTCTAGGCTTTTCAGTCGCCAACTCTTCCCCCTTAGTTTTACATAGCCGCAGCAGCAGCAGGCGTGCCTCGTTTTTCAAATCTCATCTTTCTGCTCGAGGCTCTGTTCCCCCAGTGAATGCCAAAGAGGATAATGTAGATTCTAGTGGAGCAGATCAACAACATTTGCCCAGTTTGAGGACCCTCCTTAAAGTTTACAAGGATGCTATTTTCAATGAAGATGAAAAGACTGTATCCGAAATTGAGGCAAGGATAGAAGTAATAGAAAATAAGCAGAATGAATTAGTCCAGAAAGTATCATCTATATCTGCAGAGGTAACATCAGGGAAGGAAAAGCTTATCCGCTTGCAAGCAGATTTTGATAATTGTAGAAAAAGATTTGAGAAAGAGAGACTAACTGTGAGGACTGATGCTCAAGGAGAAGTAATTGAGAGCCTTTTGCCCATGGTTGACAATTTTGAGAGAGCAAAACAACAGATTAGACCTGAAACTGAAATGGAGAAGAAGATTGATACAAGTTACCAAGGTATATACAAGCAATTTGTTGAGATTATGAGGAGCTTGCGTGTGGCCTCTGTGCCAACTAAGGGAAAGCCCTTTGATCCTTCGGTGCATGAAGCTATTGCAAGAGAAGAGTCTCAAGAATTTCCTGAGGGGATTGTCATTCAAGAGATTCGCCGTGGCTTTTTACTTGGAGGTAGACTTCTAAGACCAGCAATGGTTAAAGTCTCTATAGGGCCTGGCAGAAAGAAATCCCCTGTGGCCACTGAGAAATCATCAGGGTCACCTGCAACAGCCGCTTCGGTAGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

304

Amino Acids

33.62

Weight (kDa)

9.17

Isoelectric Point (pI)

57.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 121 - 277 1.6e-39 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 895
Acc36I ACCTGC 1 cut(s) 895
AccI GTMKAC 1 cut(s) 799
AciI CCGC 3 cut(s) 148, 442, 897
AclWI GGATC 1 cut(s) 695
AcoI YGGCCR 1 cut(s) 861
AcsI RAATTY 1 cut(s) 743
AfiI CCNNNNNNNGG 3 cut(s) 104, 276, 686
AgsI TTSAA 3 cut(s) 178, 313, 767
AjnI CCWGG 1 cut(s) 838
AluBI AGCT 3 cut(s) 436, 660, 719
AluI AGCT 3 cut(s) 436, 660, 719
Alw26I GTCTC 3 cut(s) 484, 741, 832
AlwI GGATC 1 cut(s) 695
Ama87I CYCGRG 1 cut(s) 195
AoxI GGCC 3 cut(s) 669, 836, 861
ApeKI GCWGC 3 cut(s) 150, 153, 156
ApoI RAATTY 1 cut(s) 743
AspS9I GGNCC 2 cut(s) 279, 836
AsuHPI GGTGA 1 cut(s) 876
AvaI CYCGRG 1 cut(s) 195
AvaII GGWCC 1 cut(s) 279
AxyI CCTNAGG 1 cut(s) 750
BalI TGGCCA 1 cut(s) 863
BbvI GCAGC 3 cut(s) 162, 165, 168
BceAI ACGGC 1 cut(s) 762
BciT130I CCWGG 1 cut(s) 840
BciVI GTATCC 1 cut(s) 343
BcoDI GTCTC 3 cut(s) 484, 741, 832
BfaI CTAG 2 cut(s) 104, 243
BfmI CTRYAG 2 cut(s) 408, 831
BfuAI ACCTGC 1 cut(s) 895
BfuI GTATCC 1 cut(s) 343
BisI GCNGC 5 cut(s) 148, 151, 154, 157, 897
BlsI GCNGC 5 cut(s) 149, 152, 155, 158, 898
Bme1390I CCNGG 1 cut(s) 840
Bme18I GGWCC 1 cut(s) 279
BmeT110I CYCGRG 1 cut(s) 195
BmgT120I GGNCC 2 cut(s) 279, 836
BmiI GGNNCC 1 cut(s) 281
BmrFI CCNGG 1 cut(s) 840
BmrI ACTGGG 2 cut(s) 206, 264
BmsI GCATC 2 cut(s) 292, 499
BmuI ACTGGG 2 cut(s) 206, 264
BpuEI CTTGAG 2 cut(s) 498, 723
BsaJI CCNNGG 3 cut(s) 543, 622, 778
BsaXI ACNNNNNCTCC 2 cut(s) 649, 679
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 276, 686
Bse1I ACTGG 2 cut(s) 212, 270
Bse21I CCTNAGG 1 cut(s) 750
Bse3DI GCAATG 2 cut(s) 78, 822
BseBI CCWGG 1 cut(s) 840
BseDI CCNNGG 3 cut(s) 543, 622, 778
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 3 cut(s) 104, 276, 686
BseMI GCAATG 2 cut(s) 78, 822
BseMII CTCAG 2 cut(s) 741, 858
BseNI ACTGG 2 cut(s) 212, 270
BseRI GAGGAG 6 cut(s) 36, 58, 75, 78, 81, 670
BseXI GCAGC 3 cut(s) 162, 165, 168
BshFI GGCC 3 cut(s) 671, 838, 863
BsiHKCI CYCGRG 1 cut(s) 195
BslI CCNNNNNNNGG 3 cut(s) 104, 276, 686
BsmAI GTCTC 3 cut(s) 484, 741, 832
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 3 cut(s) 671, 838, 863
BsoBI CYCGRG 1 cut(s) 195
Bsp143I GATC 2 cut(s) 253, 700
Bsp19I CCATGG 1 cut(s) 543
BspACI CCGC 3 cut(s) 148, 442, 897
BspANI GGCC 3 cut(s) 671, 838, 863
BspCNI CTCAG 2 cut(s) 742, 859
BspLI GGNNCC 1 cut(s) 281
BspMAI CTGCAG 1 cut(s) 412
BspMI ACCTGC 1 cut(s) 895
BspPI GGATC 1 cut(s) 695
BsrDI GCAATG 2 cut(s) 78, 822
BsrI ACTGG 2 cut(s) 212, 270
BssECI CCNNGG 3 cut(s) 543, 622, 778
BssMI GATC 2 cut(s) 253, 700
BssT1I CCWWGG 2 cut(s) 543, 622
Bst2UI CCWGG 1 cut(s) 840
Bst4CI ACNGT 2 cut(s) 331, 499
Bst6I CTCTTC 3 cut(s) 105, 130, 726
BstC8I GCNNGC 5 cut(s) 161, 165, 446, 450, 662
BstDEI CTNAG 5 cut(s) 133, 684, 750, 806, 867
BstDSI CCRYGG 2 cut(s) 543, 778
BstEII GGTNACC 1 cut(s) 882
BstENI CCTNNNNNAGG 1 cut(s) 102
BstF5I GGATG 1 cut(s) 307
BstKTI GATC 2 cut(s) 256, 703
BstMAI GTCTC 3 cut(s) 484, 741, 832
BstMBI GATC 2 cut(s) 253, 700
BstMWI GCNNNNNNNGC 3 cut(s) 153, 156, 896
BstNI CCWGG 1 cut(s) 840
BstPI GGTNACC 1 cut(s) 882
BstSCI CCNGG 1 cut(s) 838
BstSFI CTRYAG 2 cut(s) 408, 831
BstV1I GCAGC 3 cut(s) 162, 165, 168
Bsu36I CCTNAGG 1 cut(s) 750
BsuI GTATCC 1 cut(s) 343
BsuRI GGCC 3 cut(s) 671, 838, 863
BtgI CCRYGG 2 cut(s) 543, 778
BtsCI GGATG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 219, 864
BveI ACCTGC 1 cut(s) 895
Cac8I GCNNGC 5 cut(s) 161, 165, 446, 450, 662
Cfr13I GGNCC 2 cut(s) 279, 836
CviAII CATG 2 cut(s) 544, 713
DdeI CTNAG 5 cut(s) 133, 684, 750, 806, 867
DpnI GATC 2 cut(s) 255, 702
DpnII GATC 2 cut(s) 253, 700
EaeI YGGCCR 1 cut(s) 861
Eam1104I CTCTTC 3 cut(s) 105, 130, 726
EarI CTCTTC 3 cut(s) 105, 130, 726
Eco130I CCWWGG 2 cut(s) 543, 622
Eco47I GGWCC 1 cut(s) 279
Eco81I CCTNAGG 1 cut(s) 750
Eco88I CYCGRG 1 cut(s) 195
Eco91I GGTNACC 1 cut(s) 882
EcoNI CCTNNNNNAGG 1 cut(s) 102
EcoO109I RGGNCCY 2 cut(s) 279, 836
EcoO65I GGTNACC 1 cut(s) 882
EcoRII CCWGG 1 cut(s) 838
EcoT14I CCWWGG 2 cut(s) 543, 622
ErhI CCWWGG 2 cut(s) 543, 622
FaeI CATG 2 cut(s) 547, 716
FaiI YATR 9 cut(s) 41, 144, 404, 545, 629, 631, 653, 714, 833
FatI CATG 2 cut(s) 543, 712
FblI GTMKAC 1 cut(s) 799
Fnu4HI GCNGC 5 cut(s) 148, 151, 154, 157, 897
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 5 cut(s) 148, 151, 154, 157, 897
FspBI CTAG 2 cut(s) 104, 243
GluI GCNGC 5 cut(s) 148, 151, 154, 157, 897
HaeIII GGCC 3 cut(s) 671, 838, 863
Hin1II CATG 2 cut(s) 547, 716
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 3 cut(s) 239, 734, 772
HphI GGTGA 1 cut(s) 876
Hpy166II GTNNAC 3 cut(s) 295, 550, 800
Hpy188I TCNGA 1 cut(s) 337
Hpy188III TCNNGA 4 cut(s) 388, 740, 749, 767
Hpy8I GTNNAC 3 cut(s) 295, 550, 800
HpyAV CCTTC 3 cut(s) 73, 421, 714
HpyCH4III ACNGT 2 cut(s) 331, 499
HpyCH4V TGCA 5 cut(s) 410, 448, 712, 725, 890
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 156, 896
HpyF3I CTNAG 5 cut(s) 133, 684, 750, 806, 867
Hsp92II CATG 2 cut(s) 547, 716
Kzo9I GATC 2 cut(s) 253, 700
LmnI GCTCC 3 cut(s) 88, 248, 657
Lsp1109I GCAGC 3 cut(s) 162, 165, 168
LweI GCATC 2 cut(s) 292, 499
MaeI CTAG 2 cut(s) 104, 243
MaeIII GTNAC 3 cut(s) 415, 617, 882
MalI GATC 2 cut(s) 255, 702
MboI GATC 2 cut(s) 253, 700
MboII GAAGA 6 cut(s) 92, 117, 329, 613, 616, 743
MlsI TGGCCA 1 cut(s) 863
MluCI AATT 8 cut(s) 339, 380, 463, 525, 553, 638, 743, 910
MluNI TGGCCA 1 cut(s) 863
MlyI GAGTC 1 cut(s) 743
Mox20I TGGCCA 1 cut(s) 863
MscI TGGCCA 1 cut(s) 863
MseI TTAA 2 cut(s) 288, 822
Msp20I TGGCCA 1 cut(s) 863
MspR9I CCNGG 1 cut(s) 840
Mva1269I GAATGC 1 cut(s) 223
MvaI CCWGG 1 cut(s) 840
MwoI GCNNNNNNNGC 3 cut(s) 153, 156, 896
NcoI CCATGG 1 cut(s) 543
NdeII GATC 2 cut(s) 253, 700
NlaIII CATG 2 cut(s) 547, 716
NlaIV GGNNCC 1 cut(s) 281
NmuCI GTSAC 1 cut(s) 882
PaeR7I CTCGAG 1 cut(s) 195
PaqCI CACCTGC 1 cut(s) 895
PctI GAATGC 1 cut(s) 223
PfeI GAWTC 2 cut(s) 239, 772
PkrI GCNGC 5 cut(s) 149, 152, 155, 158, 898
PleI GAGTC 1 cut(s) 742
PpsI GAGTC 1 cut(s) 742
PpuMI RGGWCCY 1 cut(s) 279
Psp5II RGGWCCY 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 838
PspEI GGTNACC 1 cut(s) 882
PspGI CCWGG 1 cut(s) 838
PspN4I GGNNCC 1 cut(s) 281
PspPI GGNCC 2 cut(s) 279, 836
PspPPI RGGWCCY 1 cut(s) 279
PspXI VCTCGAGB 1 cut(s) 195
PstI CTGCAG 1 cut(s) 412
SaqAI TTAA 2 cut(s) 288, 822
SatI GCNGC 5 cut(s) 148, 151, 154, 157, 897
Sau3AI GATC 2 cut(s) 253, 700
Sau96I GGNCC 2 cut(s) 279, 836
SchI GAGTC 1 cut(s) 743
ScrFI CCNGG 1 cut(s) 840
SfaNI GCATC 2 cut(s) 292, 499
SfcI CTRYAG 2 cut(s) 408, 831
Sfr274I CTCGAG 1 cut(s) 195
SinI GGWCC 1 cut(s) 279
SlaI CTCGAG 1 cut(s) 195
SmlI CTYRAG 3 cut(s) 195, 513, 738
SmoI CTYRAG 3 cut(s) 195, 513, 738
Sse9I AATT 8 cut(s) 339, 380, 463, 525, 553, 638, 743, 910
SsiI CCGC 3 cut(s) 148, 442, 897
SspMI CTAG 2 cut(s) 104, 243
StyD4I CCNGG 1 cut(s) 838
StyI CCWWGG 2 cut(s) 543, 622
TaaI ACNGT 2 cut(s) 331, 499
TaqI TCGA 1 cut(s) 196
TasI AATT 8 cut(s) 339, 380, 463, 525, 553, 638, 743, 910
TauI GCSGC 2 cut(s) 150, 899
TfiI GAWTC 2 cut(s) 239, 772
Tru1I TTAA 2 cut(s) 288, 822
Tru9I TTAA 2 cut(s) 288, 822
TscAI CASTG 2 cut(s) 219, 871
TseFI GTSAC 1 cut(s) 882
TseI GCWGC 3 cut(s) 150, 153, 156
Tsp45I GTSAC 1 cut(s) 882
TspDTI ATGAA 4 cut(s) 330, 336, 393, 729
TspRI CASTG 2 cut(s) 219, 871
VpaK11BI GGWCC 1 cut(s) 279
XagI CCTNNNNNAGG 1 cut(s) 102
XapI RAATTY 1 cut(s) 743
XhoI CTCGAG 1 cut(s) 195
XmiI GTMKAC 1 cut(s) 799
XspI CTAG 2 cut(s) 104, 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.