RchiOBHm_Chr7g0240561

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001161
Physical Location & Seq
Reverse (-)
66514802 .. 66516401
1600 bp
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UTR
Exon/CDS
Intron
PRQ21559

Sequence Viewer

Length: 486 bp
ATGGCGAATTCGTTGTTTATCCCAAACAACTACTTTGTCATACCTCCTTCCCGTGTTTCTACTTCCTCCTCCTCAAAACCCATTGCTCCTCCTCAACCTCTTCTAGGCTTTTCAGTCGCCAACTCTTCCCCCAGCAGCAGCAGCAGGCGTGCCTCGTTTTTCAAATCTCATCTTGCTGCTCGAGGCTCTGTTCCCCCAGTGAATGCAAAAGAGGATAATGTAGATTCTAGTGGAGCAGATCAACAACATTTGCCCAGTTTGAGGACCCTCCTTAAAGTTTACAAGGATGCTATTTTCAATGAAGATGAAAAGACTGTATCCGAAATTCAGGCAAGGATAGAAGTAATAGAAAATAAGCAGAATGAATTAGTCCAGAAAGTATCATCTATATCTGCAGAGGTAACATCAGGGAAGCAAAAGCTTATCCGCTTGCTAGCAGATTTTGATAATTGTAGAAAAAATCTGAGAAAGAGAGACCTACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

161

Amino Acids

17.71

Weight (kDa)

9.63

Isoelectric Point (pI)

58.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 427
AcsI RAATTY 2 cut(s) 7, 324
AfiI CCNNNNNNNGG 2 cut(s) 104, 261
AgsI TTSAA 2 cut(s) 163, 298
AluBI AGCT 1 cut(s) 421
AluI AGCT 1 cut(s) 421
Alw26I GTCTC 1 cut(s) 468
Ama87I CYCGRG 1 cut(s) 180
ApeKI GCWGC 4 cut(s) 135, 138, 141, 176
ApoI RAATTY 2 cut(s) 7, 324
AspS9I GGNCC 1 cut(s) 264
AsuNHI GCTAGC 1 cut(s) 433
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 1 cut(s) 264
BbvI GCAGC 4 cut(s) 147, 150, 153, 163
BciVI GTATCC 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 468
BfaI CTAG 3 cut(s) 104, 228, 434
BfmI CTRYAG 1 cut(s) 393
BfuI GTATCC 1 cut(s) 328
BisI GCNGC 4 cut(s) 136, 139, 142, 177
BlsI GCNGC 4 cut(s) 137, 140, 143, 178
Bme18I GGWCC 1 cut(s) 264
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 1 cut(s) 264
BmiI GGNNCC 1 cut(s) 266
BmrI ACTGGG 2 cut(s) 191, 249
BmsI GCATC 1 cut(s) 277
BmtI GCTAGC 1 cut(s) 437
BmuI ACTGGG 2 cut(s) 191, 249
BsaI GGTCTC 1 cut(s) 468
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 261
Bse1I ACTGG 2 cut(s) 197, 255
Bse3DI GCAATG 1 cut(s) 81
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 2 cut(s) 104, 261
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 1 cut(s) 455
BseNI ACTGG 2 cut(s) 197, 255
BseRI GAGGAG 4 cut(s) 58, 61, 78, 81
BseXI GCAGC 4 cut(s) 147, 150, 153, 163
BseYI CCCAGC 1 cut(s) 131
BsiHKCI CYCGRG 1 cut(s) 180
BslI CCNNNNNNNGG 2 cut(s) 104, 261
BsmAI GTCTC 1 cut(s) 468
BsmI GAATGC 1 cut(s) 208
Bso31I GGTCTC 1 cut(s) 468
BsoBI CYCGRG 1 cut(s) 180
Bsp143I GATC 1 cut(s) 238
BspACI CCGC 1 cut(s) 427
BspCNI CTCAG 1 cut(s) 456
BspLI GGNNCC 1 cut(s) 266
BspMAI CTGCAG 1 cut(s) 397
BspOI GCTAGC 1 cut(s) 437
BspTNI GGTCTC 1 cut(s) 468
BsrDI GCAATG 1 cut(s) 81
BsrI ACTGG 2 cut(s) 197, 255
BssMI GATC 1 cut(s) 238
Bst4CI ACNGT 2 cut(s) 316, 483
Bst6I CTCTTC 2 cut(s) 105, 130
BstC8I GCNNGC 4 cut(s) 146, 150, 431, 435
BstDEI CTNAG 1 cut(s) 464
BstENI CCTNNNNNAGG 1 cut(s) 102
BstF5I GGATG 1 cut(s) 292
BstKTI GATC 1 cut(s) 241
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 1 cut(s) 238
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 4 cut(s) 147, 150, 153, 163
BsuI GTATCC 1 cut(s) 328
BtsCI GGATG 1 cut(s) 292
BtsIMutI CAGTG 1 cut(s) 204
Cac8I GCNNGC 4 cut(s) 146, 150, 431, 435
Cfr13I GGNCC 1 cut(s) 264
CviJI RGCY 3 cut(s) 108, 186, 421
CviKI_1 RGCY 3 cut(s) 108, 186, 421
DdeI CTNAG 1 cut(s) 464
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
Eam1104I CTCTTC 2 cut(s) 105, 130
EarI CTCTTC 2 cut(s) 105, 130
Eco31I GGTCTC 1 cut(s) 468
Eco47I GGWCC 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 180
EcoNI CCTNNNNNAGG 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 264
EcoRI GAATTC 1 cut(s) 7
FaiI YATR 2 cut(s) 41, 389
Fnu4HI GCNGC 4 cut(s) 136, 139, 142, 177
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 4 cut(s) 136, 139, 142, 177
FspBI CTAG 3 cut(s) 104, 228, 434
GluI GCNGC 4 cut(s) 136, 139, 142, 177
GsaI CCCAGC 1 cut(s) 135
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 1 cut(s) 224
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 2 cut(s) 322, 465
Hpy188III TCNNGA 1 cut(s) 373
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 2 cut(s) 316, 483
HpyCH4V TGCA 2 cut(s) 206, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpyF3I CTNAG 1 cut(s) 464
Kzo9I GATC 1 cut(s) 238
LmnI GCTCC 2 cut(s) 91, 233
LpnPI CCDG 7 cut(s) 130, 145, 210, 268, 314, 386, 393
Lsp1109I GCAGC 4 cut(s) 147, 150, 153, 163
LweI GCATC 1 cut(s) 277
MaeI CTAG 3 cut(s) 104, 228, 434
MaeIII GTNAC 1 cut(s) 400
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MboII GAAGA 3 cut(s) 92, 117, 314
MluCI AATT 4 cut(s) 7, 324, 365, 448
MseI TTAA 1 cut(s) 273
Mva1269I GAATGC 1 cut(s) 208
MwoI GCNNNNNNNGC 1 cut(s) 141
NdeII GATC 1 cut(s) 238
NheI GCTAGC 1 cut(s) 433
NlaIV GGNNCC 1 cut(s) 266
PaeR7I CTCGAG 1 cut(s) 180
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 224
PkrI GCNGC 4 cut(s) 137, 140, 143, 178
PpuMI RGGWCCY 1 cut(s) 264
Psp5II RGGWCCY 1 cut(s) 264
PspFI CCCAGC 1 cut(s) 131
PspN4I GGNNCC 1 cut(s) 266
PspPI GGNCC 1 cut(s) 264
PspPPI RGGWCCY 1 cut(s) 264
PspXI VCTCGAGB 1 cut(s) 180
PstI CTGCAG 1 cut(s) 397
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 4 cut(s) 136, 139, 142, 177
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 1 cut(s) 264
SetI ASST 5 cut(s) 46, 100, 402, 423, 480
SfaNI GCATC 1 cut(s) 277
SfcI CTRYAG 1 cut(s) 393
Sfr274I CTCGAG 1 cut(s) 180
SinI GGWCC 1 cut(s) 264
SlaI CTCGAG 1 cut(s) 180
SmlI CTYRAG 1 cut(s) 180
SmoI CTYRAG 1 cut(s) 180
Sse9I AATT 4 cut(s) 7, 324, 365, 448
SsiI CCGC 1 cut(s) 427
SspMI CTAG 3 cut(s) 104, 228, 434
TaaI ACNGT 2 cut(s) 316, 483
TaqI TCGA 1 cut(s) 181
TasI AATT 4 cut(s) 7, 324, 365, 448
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 1 cut(s) 204
TseI GCWGC 4 cut(s) 135, 138, 141, 176
TspDTI ATGAA 3 cut(s) 315, 321, 378
TspRI CASTG 1 cut(s) 204
VpaK11BI GGWCC 1 cut(s) 264
XagI CCTNNNNNAGG 1 cut(s) 102
XapI RAATTY 2 cut(s) 7, 324
XhoI CTCGAG 1 cut(s) 180
XspI CTAG 3 cut(s) 104, 228, 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.