FvH4_7g31310

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000753
Physical Location & Seq
Forward (+)
22657770 .. 22665170
7401 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31310.t1

Sequence Viewer

Length: 3450 bp
ATGCAGCAACTTGAGACGACAGCCGATGAGATTCGCTCCATACGGGTCGACGGGCTGGCGCCGATGATGGATGAGACCTATCTCTTCAACTGCTTCTGTGACAACGCAAAGGTTGTGGCTGACACGGTTTTGCACAATCGGCGAACAGGATTGCCGCTAGATTATGGTTTTGTGGAGTTTGCTTCTCACGGCGATGCTGAGTTTGTCTTGGATTCGTACCATGGTGAGCGGATGCCCAACTTCAATTATGAGAGATTCTGTCTGAGCTGGGACACTGATGGTTCCGGTACAAGGTGGCGCTGGGATTATACTTACGAGAGTTGCTCTTTCTTTGGTTATTACGAGGATGGTAATGAAATGGATGCCAAGGCATTGATTACGCACTACATGGACTCCATACCCTCTGTTGTTACTACAAAGCGAGCCTACCCGCAATCTGCCTACCCAAATACTTGTGGAGTTTCAGGGGAGGCTTTTCCAAATTACACAGAAAACCAGCTAGATAGTACAAAGTTGAATGGTTCAAGTCCTGCTGCCTATATGGAAACAGATGGTGACAAGCGTTTGAAGCTTGTGGACATGCCTCTCACTCATCCTAACATGTATCAACAAGGATATGTCGGCCCCAGTCTTACAATCCAACAGAACCAACCAGACCCGATGCAGTGCAATGTTGGTTGTGGTGCTGGATATGTTAATGACCAAGTTCCTAAGGGAGCTCTGGAAATGGTTTCCTCGATGAAGGAGGAGAAGGAGCTGTATCTAGGTTTCGAAGAAGTCAACTATAGAAGAAGTTGTTACGTCTTCCGCACCATCAAACTGTCAGAGTTATTGCAATGCTCTTGTCACTTTAGCATCGAAACTTGTGAAATAACCAAGTGCAAGCATTGCGCCCCCAAAATCAAACTGGAATCTCAGTGGCGCCTGCGGGCTTGGCTCCACACCGGGGAAAAAGTCCGTGAAAGCTGCGGCGCCAAAGAGACGAATATCCCAGATGGTATGATTTGCTGTTCCTACGGCTCCCAAATCATCTTCGCCGGCGGCTTGGGGAAGATGGGGAAACACCAGAGTGGCCTCCCCTTGTGCCTAGTGGACAGTAGACCCAGTGGTTCTCATGACAGCACTTTCGAGGTATACAATAGCAGTTCTCGTGAATGTTTGGCTCTGCCAGATCCTGAAGTTTACTTGCTGGGAAATGACAGTTGCAGTTATCTTGTAAACTATTTGACTCTTGATAGATTTGCTTGTGCTGTTGTTGGATCCAAGATCTTAACCTCTAACCCGGAGTTAGTGTTTCTAGCCCCTTCCGTTCCCATTATTTGCTTTGACGTAAATGACAAGGAAAAGAAATGGAGAGTGATGCCTTCCTTGTTCGATGGTGAGCCTTTTCCCTTCGTAGGCAGGGCTTTAGTCTTGGACTTGAATGGTACACATGATAAGGTCATGTTCTCCTTTCGTCATGATTGCCTGATATATGTCTCTCGATTAGCAGTGGATGATGACACCGGCGATATAAGCATCTCTCACGATGTCTGTTCGGGGGGTCCTCACTTGGTCTGTACCTTGATGAAAACTGTTTCTGGACTGTCGTCCACATTTGTTGATCTTGGAGATCAAACTAAGGTTGCCTTTGTTATGTGTGGGAGCTTATACTCTGGGACAGCGCCTCCCGGGAGGGTGCGTGTTGTTGTCGTAGTGATTGAGTATGAAGAGTTGGGTCCAAAAATATATCTCAGGAGAATAATGAGTGGTGATATAGAAGAAAATGGTGATGAACGAATTCAAAAGGCTAATGGAATTTATTTAAGTGCAAGTTTAGCATCAGTGGACTCGGCTGCCAATAACGTCTCTAAAGCTATGTTGCCTCTTTTCAAAGGGCCAAAGAGTTTTAGTAAAACCATGAAAATTTATTATGGAGTTCTGTCAATAGGCTTGAGTTCCAATAAGAAAATTCTTGACAGCCAGCGCCAGAAATCAGTCCAGCAACTGTGGAATAAGATGCTTCTTGTTCTGTGTGTCATTGCGCTCTCACTGGATCCTTTGTTTTGCTATATTCTTGTGGTTAATGATGAGAAGAAGTGTCTTCGATTGGACAGAAAATTAAGAGCTATAGCGACAGTTCTGCGAGGAGTTGTGGATTTCTTCTATCTGTTTTACATCATTTATCAATTTCGTGCTAGTTATGTCACTCTTTTTTCTAAATCATCTAGAGGAGGTAAACCGGCTCCGGATACTCAGGCAAGAGCAAGGAGGTATTTGTTTTTGTACTTCATATTCGACATCCTTGTAATTCTTCCAGTTCCACAGGGGATAGTTTATGGTATCATTCCAAGATTGAATAACTCGAGGGTTTTCCATGCAACACGTTCCTTGTACATCATAGTTATCTTTCAATACGTGCTAAGGGTTCTTCGAATGTGCTCATTGTTAAAAGAAGCTGCAAGGTCTTCTGGCATCCTCTCCGAAACTGCATGGGCGAAAGCTGCCTTTAATCTTTTTCTTTATATGCTAGCCAGTCATGTTGTTGGAGCCTTCTGGTACTTCTTTTCGATAGAAAGGAAAACATCCTGCTGGCGAAAATCTTGTAAAATGCATGCTATTGCTCATTGTTCGTTATATTGTGACGATAGTTTTGGAGCTAGCACTTTTCTGAATGATTTTTGTTCTCCAAAGACGCCAAATTCCACATTTTTTGATTTTGGAATATACTTTGGTGCTCTTGAATCTGGTGTCATTGAATCAACAGATTTCTGGACAAAGATCTCTTACTGCTTTTGGTGGGGACTGCAGAACCTGAGTTCCCTGGGTCAAAGCCTCAAAACAAGTACCTATCTGTGGGAAGTCTACTTCTCAGTTTTTGTTTCACTCACTGGATGGATACTGTTTGCATTTCTGATTGGAAATATGCAGACATATTTGCAATCAAAAACAGCAAGGCTGGAGGAAATGAGATTGAAGGGGCAAGAGATAGAGTTGTGGATGGCCTTCCATTCCCTTCCTCGGAAGCTCAAGAAGCGAATATGGCGATACCAGAAATACAAATGGCAAGAAACTAAAGGTGTTGATGTGGAGAGTTTTCTTCGCAATCTTCCCGGGATATCAGAACGCAAACAAAGCAGCATCTGTGTTCCGGTTCCAATGCTTCAAAACATGGATAAACGTTTGCTTGAGGCAATATGTGATCATCTCAAGCCAGTGCTTTATATAGAGCACAGCTTCATACTACGGAAAGGTGACCCTCTTGATGAGATGTTGTTCATCACTCGCGGCAAGGTATTGACTTACACCACCGCAGGCAGAGAAGGTGCTGATTGTCTTGAAAAGGGTGACTTCTATGGCCAAGAACTTCTTGACTGGGTGCTCAAGACTCCCAATCCTAGCCTATCCAACCTTCCATTCTCAACTAAAACAGTCCAAGCGCACACAAAAGTTGAAAGTGTTTGCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

1150

Amino Acids

130.04

Weight (kDa)

7.57

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 683 - 979 1.3e-15 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 58, 921, 971
AccBSI CCGCTC 1 cut(s) 229
AccI GTMKAC 4 cut(s) 48, 1099, 1134, 2844
AccII CGCG 1 cut(s) 3267
AccIII TCCGGA 1 cut(s) 2227
AciI CCGC 9 cut(s) 155, 229, 431, 808, 928, 969, 1041, 3267, 3291
AclI AACGTT 1 cut(s) 3160
AclWI GGATC 5 cut(s) 1166, 1254, 1267, 2030, 2043
AcoI YGGCCR 1 cut(s) 3337
AcsI RAATTY 5 cut(s) 1779, 1797, 1905, 1950, 2680
AcuI CTGAAG 1 cut(s) 1197
AcyI GRCGYC 4 cut(s) 59, 922, 972, 2675
AfaI GTAC 9 cut(s) 218, 289, 508, 1429, 1561, 2267, 2375, 2540, 2827
AfiI CCNNNNNNNGG 5 cut(s) 291, 946, 1397, 2835, 3000
AflIII ACRYGT 2 cut(s) 600, 2363
AjnI CCWGG 1 cut(s) 2802
AjuI GAANNNNNNNTTGG 2 cut(s) 3380, 3412
AleI CACNNNNGTG 1 cut(s) 1068
AloI GAACNNNNNNTCC 2 cut(s) 2783, 2815
Alw21I GWGCWC 5 cut(s) 721, 2423, 2719, 3213, 3363
Alw26I GTCTC 5 cut(s) 8, 68, 974, 1483, 1852
AlwI GGATC 5 cut(s) 1166, 1254, 1267, 2030, 2043
AlwNI CAGNNNCTG 4 cut(s) 1175, 1987, 2794, 3123
Ama87I CYCGRG 3 cut(s) 1670, 2344, 3092
Aor13HI TCCGGA 1 cut(s) 2227
AoxI GGCC 5 cut(s) 622, 1072, 1877, 2982, 3337
ApeKI GCWGC 7 cut(s) 4, 533, 966, 1835, 2438, 2483, 3117
ApoI RAATTY 5 cut(s) 1779, 1797, 1905, 1950, 2680
ArsI GACNNNNNNTTYG 4 cut(s) 1109, 1141, 2615, 2647
Asp700I GAANNNNTTC 1 cut(s) 1779
AspLEI GCGC 9 cut(s) 61, 300, 893, 924, 974, 1666, 1968, 2026, 3421
AspS9I GGNCC 4 cut(s) 623, 1544, 1718, 1877
AsuC2I CCSGG 6 cut(s) 946, 1283, 1671, 1672, 3093, 3094
AsuHPI GGTGA 7 cut(s) 236, 566, 1391, 1763, 1781, 3245, 3338
AsuII TTCGAA 2 cut(s) 771, 2413
AsuNHI GCTAGC 2 cut(s) 2509, 2639
AvaI CYCGRG 3 cut(s) 1670, 2344, 3092
AvaII GGWCC 2 cut(s) 1544, 1718
AxyI CCTNAGG 1 cut(s) 711
BalI TGGCCA 1 cut(s) 3339
BamHI GGATCC 2 cut(s) 1259, 2035
BanI GGYRCC 3 cut(s) 58, 921, 971
BanII GRGCYC 1 cut(s) 721
BauI CACGAG 1 cut(s) 1149
BbsI GAAGAC 3 cut(s) 796, 2075, 2439
Bbv12I GWGCWC 5 cut(s) 721, 2423, 2719, 3213, 3363
BbvI GCAGC 7 cut(s) 16, 520, 953, 1822, 2425, 2470, 3129
BceAI ACGGC 2 cut(s) 205, 1033
BcgI CGANNNNNNTGC 2 cut(s) 2104, 2138
BciT130I CCWGG 1 cut(s) 2804
BciVI GTATCC 2 cut(s) 2224, 2871
BclI TGATCA 1 cut(s) 3181
BcnI CCSGG 6 cut(s) 946, 1283, 1671, 1672, 3093, 3094
BcoDI GTCTC 5 cut(s) 8, 68, 974, 1483, 1852
BfmI CTRYAG 3 cut(s) 784, 2109, 2786
BfoI RGCGCY 6 cut(s) 62, 301, 925, 975, 1667, 1969
BfuI GTATCC 2 cut(s) 2224, 2871
BglII AGATCT 2 cut(s) 1266, 2760
Bme1390I CCNGG 7 cut(s) 946, 1283, 1671, 1672, 2804, 3093, 3094
Bme18I GGWCC 2 cut(s) 1544, 1718
BmeT110I CYCGRG 3 cut(s) 1670, 2344, 3092
BmgT120I GGNCC 4 cut(s) 623, 1544, 1718, 1877
BmrFI CCNGG 7 cut(s) 946, 1283, 1671, 1672, 2804, 3093, 3094
BmrI ACTGGG 3 cut(s) 621, 1098, 3364
BmtI GCTAGC 2 cut(s) 2513, 2643
BmuI ACTGGG 3 cut(s) 621, 1098, 3364
BoxI GACNNNNGTC 1 cut(s) 1588
BpiI GAAGAC 3 cut(s) 796, 2075, 2439
BplI GAGNNNNNCTC 4 cut(s) 20, 52, 308, 340
BpmI CTGGAG 1 cut(s) 2960
Bpu10I CCTNAGC 1 cut(s) 2402
Bpu14I TTCGAA 2 cut(s) 771, 2413
BpuEI CTTGAG 6 cut(s) 32, 1954, 2993, 3173, 3188, 3347
BpuMI CCSGG 6 cut(s) 946, 1283, 1671, 1672, 3093, 3094
BsaAI YACGTR 1 cut(s) 2398
BsaHI GRCGYC 4 cut(s) 59, 922, 972, 2675
BsaI GGTCTC 1 cut(s) 68
BsaJI CCNNGG 8 cut(s) 220, 366, 945, 1670, 2802, 2803, 2999, 3092
BsaWI WCCGGW 3 cut(s) 284, 2227, 3130
BsaXI ACNNNNNCTCC 8 cut(s) 377, 407, 1277, 1307, 1651, 1666, 1681, 1696
Bsc4I CCNNNNNNNGG 5 cut(s) 291, 946, 1397, 2835, 3000
Bse118I RCCGGY 3 cut(s) 1037, 1505, 2221
Bse1I ACTGG 9 cut(s) 627, 912, 1104, 2037, 2297, 2514, 2875, 3194, 3359
Bse21I CCTNAGG 1 cut(s) 711
Bse3DI GCAATG 4 cut(s) 676, 842, 886, 2019
BseAI TCCGGA 1 cut(s) 2227
BseBI CCWGG 1 cut(s) 2804
BseDI CCNNGG 8 cut(s) 220, 366, 945, 1670, 2802, 2803, 2999, 3092
BseLI CCNNNNNNNGG 5 cut(s) 291, 946, 1397, 2835, 3000
BseMI GCAATG 4 cut(s) 676, 842, 886, 2019
BseMII CTCAG 7 cut(s) 189, 254, 929, 1747, 2249, 2786, 2865
BseNI ACTGG 9 cut(s) 627, 912, 1104, 2037, 2297, 2514, 2875, 3194, 3359
BseRI GAGGAG 3 cut(s) 761, 2142, 2226
BseXI GCAGC 7 cut(s) 16, 520, 953, 1822, 2425, 2470, 3129
BseYI CCCAGC 3 cut(s) 267, 300, 1189
Bsh1236I CGCG 1 cut(s) 3267
BshFI GGCC 5 cut(s) 624, 1074, 1879, 2984, 3339
BshNI GGYRCC 3 cut(s) 58, 921, 971
BsiHKAI GWGCWC 5 cut(s) 721, 2423, 2719, 3213, 3363
BsiHKCI CYCGRG 3 cut(s) 1670, 2344, 3092
BslFI GGGAC 3 cut(s) 284, 1672, 2796
BslI CCNNNNNNNGG 5 cut(s) 291, 946, 1397, 2835, 3000
BsmAI GTCTC 5 cut(s) 8, 68, 974, 1483, 1852
BsmBI CGTCTC 3 cut(s) 8, 974, 1852
BsmFI GGGAC 3 cut(s) 284, 1672, 2796
BsnI GGCC 5 cut(s) 624, 1074, 1879, 2984, 3339
Bso31I GGTCTC 1 cut(s) 68
BsoBI CYCGRG 3 cut(s) 1670, 2344, 3092
Bsp119I TTCGAA 2 cut(s) 771, 2413
Bsp1286I GDGCHC 5 cut(s) 721, 2423, 2719, 3213, 3363
Bsp13I TCCGGA 1 cut(s) 2227
Bsp1407I TGTACA 1 cut(s) 2373
Bsp143I GATC 8 cut(s) 1171, 1259, 1266, 1603, 1612, 2035, 2760, 3181
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 9 cut(s) 155, 229, 431, 808, 928, 969, 1041, 3267, 3291
BspANI GGCC 5 cut(s) 624, 1074, 1879, 2984, 3339
BspCNI CTCAG 7 cut(s) 190, 255, 928, 1746, 2248, 2787, 2864
BspEI TCCGGA 1 cut(s) 2227
BspFNI CGCG 1 cut(s) 3267
BspHI TCATGA 2 cut(s) 1114, 1459
BspMAI CTGCAG 1 cut(s) 2790
BspOI GCTAGC 2 cut(s) 2513, 2643
BspPI GGATC 5 cut(s) 1166, 1254, 1267, 2030, 2043
BspT104I TTCGAA 2 cut(s) 771, 2413
BspT107I GGYRCC 3 cut(s) 58, 921, 971
BspTNI GGTCTC 1 cut(s) 68
BsrBI CCGCTC 1 cut(s) 229
BsrDI GCAATG 4 cut(s) 676, 842, 886, 2019
BsrFI RCCGGY 3 cut(s) 1037, 1505, 2221
BsrGI TGTACA 1 cut(s) 2373
BsrI ACTGG 9 cut(s) 627, 912, 1104, 2037, 2297, 2514, 2875, 3194, 3359
BssAI RCCGGY 3 cut(s) 1037, 1505, 2221
BssECI CCNNGG 8 cut(s) 220, 366, 945, 1670, 2802, 2803, 2999, 3092
BssMI GATC 8 cut(s) 1171, 1259, 1266, 1603, 1612, 2035, 2760, 3181
BssNAI GTATAC 1 cut(s) 1135
BssNI GRCGYC 4 cut(s) 59, 922, 972, 2675
BssSI CACGAG 1 cut(s) 1149
BssT1I CCWWGG 2 cut(s) 220, 366
Bst1107I GTATAC 1 cut(s) 1135
Bst2BI CACGAG 1 cut(s) 1149
Bst2UI CCWGG 1 cut(s) 2804
Bst6I CTCTTC 2 cut(s) 89, 1704
BstACI GRCGYC 4 cut(s) 59, 922, 972, 2675
BstAPI GCANNNNNTGC 1 cut(s) 888
BstAUI TGTACA 1 cut(s) 2373
BstBAI YACGTR 1 cut(s) 2398
BstBI TTCGAA 2 cut(s) 771, 2413
BstDSI CCRYGG 1 cut(s) 220
BstEII GGTNACC 1 cut(s) 3233
BstFNI CGCG 1 cut(s) 3267
BstH2I RGCGCY 6 cut(s) 62, 301, 925, 975, 1667, 1969
BstHHI GCGC 9 cut(s) 61, 300, 893, 924, 974, 1666, 1968, 2026, 3421
BstKTI GATC 8 cut(s) 1174, 1262, 1269, 1606, 1615, 2038, 2763, 3184
BstMAI GTCTC 5 cut(s) 8, 68, 974, 1483, 1852
BstMBI GATC 8 cut(s) 1171, 1259, 1266, 1603, 1612, 2035, 2760, 3181
BstNI CCWGG 1 cut(s) 2804
BstNSI RCATGY 3 cut(s) 583, 604, 2597
BstPAI GACNNNNGTC 1 cut(s) 1588
BstPI GGTNACC 1 cut(s) 3233
BstSCI CCNGG 7 cut(s) 944, 1281, 1669, 1670, 2802, 3091, 3092
BstSFI CTRYAG 3 cut(s) 784, 2109, 2786
BstUI CGCG 1 cut(s) 3267
BstV1I GCAGC 7 cut(s) 16, 520, 953, 1822, 2425, 2470, 3129
BstV2I GAAGAC 3 cut(s) 796, 2075, 2439
BstX2I RGATCY 5 cut(s) 1171, 1259, 1266, 2035, 2760
BstYI RGATCY 5 cut(s) 1171, 1259, 1266, 2035, 2760
BstZ17I GTATAC 1 cut(s) 1135
Bsu36I CCTNAGG 1 cut(s) 711
BsuI GTATCC 2 cut(s) 2224, 2871
BsuRI GGCC 5 cut(s) 624, 1074, 1879, 2984, 3339
BtgI CCRYGG 1 cut(s) 220
BtgZI GCGATG 1 cut(s) 207
BtsI GCAGTG 2 cut(s) 671, 1497
BtsIMutI CAGTG 9 cut(s) 273, 671, 923, 1111, 1497, 1830, 2030, 2868, 3201
CaiI CAGNNNCTG 4 cut(s) 1175, 1987, 2794, 3123
CciI TCATGA 2 cut(s) 1114, 1459
CfoI GCGC 9 cut(s) 61, 300, 893, 924, 974, 1666, 1968, 2026, 3421
Cfr10I RCCGGY 3 cut(s) 1037, 1505, 2221
Cfr13I GGNCC 4 cut(s) 623, 1544, 1718, 1877
Cfr9I CCCGGG 2 cut(s) 1670, 3092
CseI GACGC 1 cut(s) 2683
Csp6I GTAC 9 cut(s) 217, 288, 507, 1428, 1560, 2266, 2374, 2539, 2826
CspCI CAANNNNNGTGG 6 cut(s) 96, 131, 1582, 1617, 2674, 2709
CviQI GTAC 9 cut(s) 217, 288, 507, 1428, 1560, 2266, 2374, 2539, 2826
DinI GGCGCC 3 cut(s) 60, 923, 973
DpnI GATC 8 cut(s) 1173, 1261, 1268, 1605, 1614, 2037, 2762, 3183
DpnII GATC 8 cut(s) 1171, 1259, 1266, 1603, 1612, 2035, 2760, 3181
EaeI YGGCCR 1 cut(s) 3337
Eam1104I CTCTTC 2 cut(s) 89, 1704
EarI CTCTTC 2 cut(s) 89, 1704
Ecl136II GAGCTC 1 cut(s) 719
Eco130I CCWWGG 2 cut(s) 220, 366
Eco24I GRGCYC 1 cut(s) 721
Eco31I GGTCTC 1 cut(s) 68
Eco32I GATATC 1 cut(s) 3099
Eco47I GGWCC 2 cut(s) 1544, 1718
Eco53kI GAGCTC 1 cut(s) 719
Eco57I CTGAAG 1 cut(s) 1197
Eco81I CCTNAGG 1 cut(s) 711
Eco88I CYCGRG 3 cut(s) 1670, 2344, 3092
Eco91I GGTNACC 1 cut(s) 3233
EcoICRI GAGCTC 1 cut(s) 719
EcoO109I RGGNCCY 1 cut(s) 1544
EcoO65I GGTNACC 1 cut(s) 3233
EcoRI GAATTC 1 cut(s) 1779
EcoRII CCWGG 1 cut(s) 2802
EcoRV GATATC 1 cut(s) 3099
EcoT14I CCWWGG 2 cut(s) 220, 366
EcoT22I ATGCAT 1 cut(s) 2595
EcoT38I GRGCYC 1 cut(s) 721
EgeI GGCGCC 3 cut(s) 60, 923, 973
EheI GGCGCC 3 cut(s) 60, 923, 973
ErhI CCWWGG 2 cut(s) 220, 366
Esp3I CGTCTC 3 cut(s) 8, 974, 1852
FalI AAGNNNNNCTT 6 cut(s) 1613, 1645, 3314, 3346, 3333, 3365
FaqI GGGAC 3 cut(s) 284, 1672, 2796
FauI CCCGC 2 cut(s) 438, 921
FbaI TGATCA 1 cut(s) 3181
FblI GTMKAC 4 cut(s) 48, 1099, 1134, 2844
FriOI GRGCYC 1 cut(s) 721
GlaI GCGC 9 cut(s) 60, 299, 892, 923, 973, 1665, 1967, 2025, 3420
GsaI CCCAGC 3 cut(s) 271, 304, 1193
GsuI CTGGAG 1 cut(s) 2960
HaeII RGCGCY 6 cut(s) 62, 301, 925, 975, 1667, 1969
HaeIII GGCC 5 cut(s) 624, 1074, 1879, 2984, 3339
HgaI GACGC 1 cut(s) 2683
HhaI GCGC 9 cut(s) 61, 300, 893, 924, 974, 1666, 1968, 2026, 3421
Hin1I GRCGYC 4 cut(s) 59, 922, 972, 2675
Hin6I GCGC 9 cut(s) 59, 298, 891, 922, 972, 1664, 1966, 2024, 3419
HinP1I GCGC 9 cut(s) 59, 298, 891, 922, 972, 1664, 1966, 2024, 3419
HincII GTYRAC 2 cut(s) 49, 781
HindII GTYRAC 2 cut(s) 49, 781
HindIII AAGCTT 1 cut(s) 569
HphI GGTGA 7 cut(s) 236, 566, 1391, 1763, 1781, 3245, 3338
Hpy188I TCNGA 7 cut(s) 264, 826, 2464, 2652, 2895, 3003, 3103
Hpy99I CGWCG 1 cut(s) 53
HpyCH4IV ACGT 6 cut(s) 801, 1329, 1845, 2365, 2397, 3160
HpySE526I ACGT 6 cut(s) 801, 1329, 1845, 2365, 2397, 3160
Hsp92I GRCGYC 4 cut(s) 59, 922, 972, 2675
HspAI GCGC 9 cut(s) 59, 298, 891, 922, 972, 1664, 1966, 2024, 3419
KasI GGCGCC 3 cut(s) 58, 921, 971
Kpn2I TCCGGA 1 cut(s) 2227
KroI GCCGGC 1 cut(s) 1037
KroNI GCCGGC 1 cut(s) 1039
Ksp22I TGATCA 1 cut(s) 3181
Kzo9I GATC 8 cut(s) 1171, 1259, 1266, 1603, 1612, 2035, 2760, 3181
LmnI GCTCC 9 cut(s) 41, 716, 754, 942, 1025, 1644, 2230, 2528, 2636
Lsp1109I GCAGC 7 cut(s) 16, 520, 953, 1822, 2425, 2470, 3129
MaeII ACGT 6 cut(s) 801, 1329, 1845, 2365, 2397, 3160
MaeIII GTNAC 9 cut(s) 98, 409, 554, 797, 845, 2185, 2621, 3233, 3326
MalI GATC 8 cut(s) 1173, 1261, 1268, 1605, 1614, 2037, 2762, 3183
MbiI CCGCTC 1 cut(s) 229
MboI GATC 8 cut(s) 1171, 1259, 1266, 1603, 1612, 2035, 2760, 3181
MflI RGATCY 5 cut(s) 1171, 1259, 1266, 2035, 2760
MhlI GDGCHC 5 cut(s) 721, 2423, 2719, 3213, 3363
MlsI TGGCCA 1 cut(s) 3339
MluNI TGGCCA 1 cut(s) 3339
Mly113I GGCGCC 3 cut(s) 59, 922, 972
MlyI GAGTC 4 cut(s) 386, 1222, 1823, 3361
MmeI TCCRAC 4 cut(s) 664, 1237, 2506, 3411
Mox20I TGGCCA 1 cut(s) 3339
Mph1103I ATGCAT 1 cut(s) 2595
MreI CGCCGGCG 1 cut(s) 1037
MroI TCCGGA 1 cut(s) 2227
MroNI GCCGGC 1 cut(s) 1037
MroXI GAANNNNTTC 1 cut(s) 1779
MscI TGGCCA 1 cut(s) 3339
MseI TTAA 7 cut(s) 696, 1271, 1805, 2064, 2102, 2429, 2490
MslI CAYNNNNRTG 2 cut(s) 192, 1068
Msp20I TGGCCA 1 cut(s) 3339
MspR9I CCNGG 7 cut(s) 946, 1283, 1671, 1672, 2804, 3093, 3094
MvaI CCWGG 1 cut(s) 2804
MvnI CGCG 1 cut(s) 3267
NaeI GCCGGC 1 cut(s) 1039
NarI GGCGCC 3 cut(s) 59, 922, 972
NciI CCSGG 6 cut(s) 946, 1283, 1671, 1672, 3093, 3094
NcoI CCATGG 1 cut(s) 220
NdeII GATC 8 cut(s) 1171, 1259, 1266, 1603, 1612, 2035, 2760, 3181
NgoMIV GCCGGC 1 cut(s) 1037
NheI GCTAGC 2 cut(s) 2509, 2639
NmeAIII GCCGAG 1 cut(s) 1811
NmuCI GTSAC 7 cut(s) 98, 554, 845, 2185, 2621, 3233, 3326
NsiI ATGCAT 1 cut(s) 2595
NspI RCATGY 3 cut(s) 583, 604, 2597
NspV TTCGAA 2 cut(s) 771, 2413
OliI CACNNNNGTG 1 cut(s) 1068
PaeI GCATGC 1 cut(s) 2597
PaeR7I CTCGAG 1 cut(s) 2344
PagI TCATGA 2 cut(s) 1114, 1459
PasI CCCWGGG 1 cut(s) 2803
PciI ACATGT 1 cut(s) 600
PdiI GCCGGC 1 cut(s) 1039
PdmI GAANNNNTTC 1 cut(s) 1779
PfeI GAWTC 6 cut(s) 31, 212, 255, 911, 2723, 2738
PleI GAGTC 4 cut(s) 386, 1222, 1823, 3361
PluTI GGCGCC 3 cut(s) 62, 925, 975
PpsI GAGTC 4 cut(s) 386, 1222, 1823, 3361
Ppu21I YACGTR 1 cut(s) 2398
PpuMI RGGWCCY 1 cut(s) 1544
PscI ACATGT 1 cut(s) 600
PshAI GACNNNNGTC 1 cut(s) 1588
Psp124BI GAGCTC 1 cut(s) 721
Psp1406I AACGTT 1 cut(s) 3160
Psp5II RGGWCCY 1 cut(s) 1544
Psp6I CCWGG 1 cut(s) 2802
PspEI GGTNACC 1 cut(s) 3233
PspFI CCCAGC 3 cut(s) 267, 300, 1189
PspGI CCWGG 1 cut(s) 2802
PspPI GGNCC 4 cut(s) 623, 1544, 1718, 1877
PspPPI RGGWCCY 1 cut(s) 1544
PspXI VCTCGAGB 1 cut(s) 2344
PstI CTGCAG 1 cut(s) 2790
PstNI CAGNNNCTG 4 cut(s) 1175, 1987, 2794, 3123
PsuI RGATCY 5 cut(s) 1171, 1259, 1266, 2035, 2760
RsaI GTAC 9 cut(s) 218, 289, 508, 1429, 1561, 2267, 2375, 2540, 2827
RsaNI GTAC 9 cut(s) 217, 288, 507, 1428, 1560, 2266, 2374, 2539, 2826
RseI CAYNNNNRTG 2 cut(s) 192, 1068
SacI GAGCTC 1 cut(s) 721
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 7 cut(s) 696, 1271, 1805, 2064, 2102, 2429, 2490
Sau3AI GATC 8 cut(s) 1171, 1259, 1266, 1603, 1612, 2035, 2760, 3181
Sau96I GGNCC 4 cut(s) 623, 1544, 1718, 1877
SchI GAGTC 4 cut(s) 386, 1222, 1823, 3361
ScrFI CCNGG 7 cut(s) 946, 1283, 1671, 1672, 2804, 3093, 3094
SduI GDGCHC 5 cut(s) 721, 2423, 2719, 3213, 3363
SfcI CTRYAG 3 cut(s) 784, 2109, 2786
SfoI GGCGCC 3 cut(s) 60, 923, 973
Sfr274I CTCGAG 1 cut(s) 2344
SfuI TTCGAA 2 cut(s) 771, 2413
SgrAI CRCCGGYG 2 cut(s) 1037, 1505
SinI GGWCC 2 cut(s) 1544, 1718
SlaI CTCGAG 1 cut(s) 2344
SmaI CCCGGG 2 cut(s) 1672, 3094
SmiMI CAYNNNNRTG 2 cut(s) 192, 1068
SmlI CTYRAG 7 cut(s) 11, 1933, 2344, 3008, 3167, 3188, 3362
SmoI CTYRAG 7 cut(s) 11, 1933, 2344, 3008, 3167, 3188, 3362
SphI GCATGC 1 cut(s) 2597
SsiI CCGC 9 cut(s) 155, 229, 431, 808, 928, 969, 1041, 3267, 3291
SspDI GGCGCC 3 cut(s) 58, 921, 971
SstI GAGCTC 1 cut(s) 721
StyD4I CCNGG 7 cut(s) 944, 1281, 1669, 1670, 2802, 3091, 3092
StyI CCWWGG 2 cut(s) 220, 366
TaiI ACGT 6 cut(s) 804, 1332, 1848, 2368, 2400, 3163
TatI WGTACW 3 cut(s) 506, 2265, 2373
TauI GCSGC 4 cut(s) 157, 972, 1044, 3270
TfiI GAWTC 6 cut(s) 31, 212, 255, 911, 2723, 2738
Tru1I TTAA 7 cut(s) 696, 1271, 1805, 2064, 2102, 2429, 2490
Tru9I TTAA 7 cut(s) 696, 1271, 1805, 2064, 2102, 2429, 2490
TscAI CASTG 9 cut(s) 280, 671, 923, 1111, 1497, 1830, 2037, 2875, 3201
TseFI GTSAC 7 cut(s) 98, 554, 845, 2185, 2621, 3233, 3326
TseI GCWGC 7 cut(s) 4, 533, 966, 1835, 2438, 2483, 3117
Tsp45I GTSAC 7 cut(s) 98, 554, 845, 2185, 2621, 3233, 3326
TspDTI ATGAA 9 cut(s) 369, 755, 1583, 1722, 1788, 1916, 2260, 3208, 3247
TspGWI ACGGA 3 cut(s) 947, 1297, 3241
TspMI CCCGGG 2 cut(s) 1670, 3092
TspRI CASTG 9 cut(s) 280, 671, 923, 1111, 1497, 1830, 2037, 2875, 3201
VpaK11BI GGWCC 2 cut(s) 1544, 1718
XapI RAATTY 5 cut(s) 1779, 1797, 1905, 1950, 2680
XbaI TCTAGA 1 cut(s) 2207
XceI RCATGY 3 cut(s) 583, 604, 2597
XcmI CCANNNNNNNNNTGG 1 cut(s) 904
XhoI CTCGAG 1 cut(s) 2344
XmaI CCCGGG 2 cut(s) 1670, 3092
XmiI GTMKAC 4 cut(s) 48, 1099, 1134, 2844
XmnI GAANNNNTTC 1 cut(s) 1779
Zsp2I ATGCAT 1 cut(s) 2595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.