RLG00000026260

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000753
Physical Location & Seq
Reverse (-)
1705106 .. 1707437
2332 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026260

Sequence Viewer

Length: 939 bp
ATGATCCCGAGTGGATATGGCTTCCTTCCGACATCAAAAGCGGGAAACCGGAGGCCCGGCCTGCTGGTTAACGTTGATGGAAGTCTCTATTGTCTCTCTGGTTTACTCATTTATCAGCCTGTCGTTCATCCTACGTTTGAGATGTACAATCTCCAGTCCGGGATATGGGTGCCTCTGCCGGATCCTCCGTTTTACACACCAGAAGCTCTGAAGTACAAACGCGGTGACCCTAGGGCTGGTACACTCTTGTCTTATGCAATCATGGGCCCCAAGCTTTTGGTCTCGAGCAAGATCCCTGGTTCAGGTTCCTGCCCTCACTTTCCCGTTTGTTGCTTTGACGTTAAGGAAAAAAAGTGGACAGAGGTCACTACCTTGTTCAATGGTAAGCCTTTTCCCTTCCGACGCGGGGCTTTAGTGGTCGACTTGGAGGATGGTACCCATGATAAGGTCATGTTTTACTTCAATAATTATTTTGCTATAAGAGTCTGCCGATTAGTAGTTAATGAGGATGATGGTAGTATTAAGAACTCACCGCACCAATCTTTGGGGATTGATACCCTCATGACGAGCTTATTGGAAATTAGAATTCAAAATCAGTCAATGGTAAGAATGTCGTGGGATTTTGTTGATCTAGGAAATAAAAAAGTCTGCTTTGTTTTGTCCTCGAGCCTCCCGAAAGTTAGAGCCGACTATAGCGCCACTCATCGCCAGACGATTAGTCTTCTTGTCTTGGTAATTCAATTTCAAGTTTTGGAGTCGGGAGATTTCTCCTTCAAGTTCCTGGCTACTTGCACCTTTGAATACCAGGGCCATTTCCCTTTCATTTGTTACCCTAATCTGCAGGGTGAAGACTTGAATGCCATGAAAGTGATCAAGACATACCCGAACTGCATTGCTGTTGTAACAGAAAAGCCAGCTGCTGGTGGTCAGCAACATTAG

Protein Analysis

313

Amino Acids

34.91

Weight (kDa)

8.49

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 9 - 137 1.1e-07 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 434
AccB1I GGYRCC 2 cut(s) 169, 434
AccB7I CCANNNNNTGG 2 cut(s) 544, 920
AccI GTMKAC 1 cut(s) 420
AccII CGCG 2 cut(s) 222, 405
AciI CCGC 4 cut(s) 41, 222, 405, 533
AclI AACGTT 1 cut(s) 72
AclWI GGATC 3 cut(s) 176, 189, 286
AcsI RAATTY 1 cut(s) 585
AcuI CTGAAG 1 cut(s) 230
AfaI GTAC 4 cut(s) 146, 215, 241, 436
AfiI CCNNNNNNNGG 7 cut(s) 165, 236, 302, 406, 445, 544, 920
AgsI TTSAA 8 cut(s) 379, 463, 590, 740, 746, 775, 800, 856
AhdI GACNNNNNGTC 1 cut(s) 717
AjnI CCWGG 3 cut(s) 295, 780, 804
AluBI AGCT 4 cut(s) 206, 274, 570, 917
AluI AGCT 4 cut(s) 206, 274, 570, 917
Alw26I GTCTC 3 cut(s) 89, 98, 286
AlwI GGATC 3 cut(s) 176, 189, 286
AlwNI CAGNNNCTG 1 cut(s) 920
Ama87I CYCGRG 3 cut(s) 7, 283, 664
AoxI GGCC 4 cut(s) 53, 58, 265, 808
ApaI GGGCCC 1 cut(s) 269
ApeKI GCWGC 1 cut(s) 917
ApoI RAATTY 1 cut(s) 585
Asp718I GGTACC 1 cut(s) 434
AspA2I CCTAGG 1 cut(s) 230
AspLEI GCGC 1 cut(s) 698
AspS9I GGNCC 4 cut(s) 54, 265, 266, 808
AsuC2I CCSGG 2 cut(s) 57, 160
AsuHPI GGTGA 3 cut(s) 236, 522, 857
AvaI CYCGRG 3 cut(s) 7, 283, 664
AvrII CCTAGG 1 cut(s) 230
BaeGI GKGCMC 1 cut(s) 269
BamHI GGATCC 1 cut(s) 181
BanI GGYRCC 2 cut(s) 169, 434
BanII GRGCYC 1 cut(s) 269
BbsI GAAGAC 2 cut(s) 713, 855
BbvI GCAGC 1 cut(s) 904
BccI CCATC 3 cut(s) 71, 425, 506
BciT130I CCWGG 3 cut(s) 297, 782, 806
BclI TGATCA 1 cut(s) 870
BcnI CCSGG 2 cut(s) 57, 160
BcoDI GTCTC 3 cut(s) 89, 98, 286
BfaI CTAG 2 cut(s) 231, 632
BfmI CTRYAG 2 cut(s) 691, 839
BfoI RGCGCY 1 cut(s) 699
BisI GCNGC 1 cut(s) 918
BlnI CCTAGG 1 cut(s) 230
BlsI GCNGC 1 cut(s) 919
Bme1390I CCNGG 5 cut(s) 57, 160, 297, 782, 806
BmeRI GACNNNNNGTC 1 cut(s) 717
BmeT110I CYCGRG 3 cut(s) 7, 283, 664
BmgT120I GGNCC 4 cut(s) 54, 265, 266, 808
BmiI GGNNCC 6 cut(s) 171, 183, 267, 268, 307, 436
BmrFI CCNGG 5 cut(s) 57, 160, 297, 782, 806
BoxI GACNNNNGTC 1 cut(s) 362
BpiI GAAGAC 2 cut(s) 713, 855
BpmI CTGGAG 1 cut(s) 137
BpuMI CCSGG 2 cut(s) 57, 160
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 3 cut(s) 230, 295, 805
BsaWI WCCGGW 1 cut(s) 48
Bsc4I CCNNNNNNNGG 7 cut(s) 165, 236, 302, 406, 445, 544, 920
Bse1I ACTGG 1 cut(s) 154
Bse3DI GCAATG 1 cut(s) 891
BseBI CCWGG 3 cut(s) 297, 782, 806
BseDI CCNNGG 3 cut(s) 230, 295, 805
BseGI GGATG 3 cut(s) 127, 436, 514
BseLI CCNNNNNNNGG 7 cut(s) 165, 236, 302, 406, 445, 544, 920
BseMI GCAATG 1 cut(s) 891
BseNI ACTGG 1 cut(s) 154
BseSI GKGCMC 1 cut(s) 269
BseXI GCAGC 1 cut(s) 904
Bsh1236I CGCG 2 cut(s) 222, 405
BshFI GGCC 4 cut(s) 55, 60, 267, 810
BshNI GGYRCC 2 cut(s) 169, 434
BsiHKCI CYCGRG 3 cut(s) 7, 283, 664
BsiSI CCGG 4 cut(s) 49, 57, 159, 179
BslI CCNNNNNNNGG 7 cut(s) 165, 236, 302, 406, 445, 544, 920
BsmAI GTCTC 3 cut(s) 89, 98, 286
BsmI GAATGC 1 cut(s) 862
BsnI GGCC 4 cut(s) 55, 60, 267, 810
Bso31I GGTCTC 1 cut(s) 286
BsoBI CYCGRG 3 cut(s) 7, 283, 664
Bsp120I GGGCCC 1 cut(s) 265
Bsp1286I GDGCHC 1 cut(s) 269
Bsp1407I TGTACA 1 cut(s) 144
Bsp143I GATC 5 cut(s) 3, 181, 291, 628, 870
BspACI CCGC 4 cut(s) 41, 222, 405, 533
BspANI GGCC 4 cut(s) 55, 60, 267, 810
BspFNI CGCG 2 cut(s) 222, 405
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 6 cut(s) 171, 183, 267, 268, 307, 436
BspMAI CTGCAG 1 cut(s) 843
BspPI GGATC 3 cut(s) 176, 189, 286
BspT107I GGYRCC 2 cut(s) 169, 434
BspTNI GGTCTC 1 cut(s) 286
BsrDI GCAATG 1 cut(s) 891
BsrGI TGTACA 1 cut(s) 144
BsrI ACTGG 1 cut(s) 154
BssECI CCNNGG 3 cut(s) 230, 295, 805
BssMI GATC 5 cut(s) 3, 181, 291, 628, 870
BssT1I CCWWGG 1 cut(s) 230
Bst2UI CCWGG 3 cut(s) 297, 782, 806
BstAUI TGTACA 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 62, 915
BstEII GGTNACC 1 cut(s) 224
BstENI CCTNNNNNAGG 1 cut(s) 300
BstF5I GGATG 3 cut(s) 127, 436, 514
BstFNI CGCG 2 cut(s) 222, 405
BstH2I RGCGCY 1 cut(s) 699
BstHHI GCGC 1 cut(s) 698
BstKTI GATC 5 cut(s) 6, 184, 294, 631, 873
BstMAI GTCTC 3 cut(s) 89, 98, 286
BstMBI GATC 5 cut(s) 3, 181, 291, 628, 870
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstNI CCWGG 3 cut(s) 297, 782, 806
BstPAI GACNNNNGTC 1 cut(s) 362
BstPI GGTNACC 1 cut(s) 224
BstSCI CCNGG 5 cut(s) 55, 158, 295, 780, 804
BstSFI CTRYAG 2 cut(s) 691, 839
BstSLI GKGCMC 1 cut(s) 269
BstUI CGCG 2 cut(s) 222, 405
BstV1I GCAGC 1 cut(s) 904
BstV2I GAAGAC 2 cut(s) 713, 855
BstX2I RGATCY 2 cut(s) 181, 291
BstXI CCANNNNNNTGG 1 cut(s) 277
BstYI RGATCY 2 cut(s) 181, 291
BsuRI GGCC 4 cut(s) 55, 60, 267, 810
BtgZI GCGATG 1 cut(s) 689
BtsCI GGATG 3 cut(s) 127, 436, 514
Cac8I GCNNGC 2 cut(s) 62, 915
CaiI CAGNNNCTG 1 cut(s) 920
CciI TCATGA 1 cut(s) 561
CfoI GCGC 1 cut(s) 698
Cfr13I GGNCC 4 cut(s) 54, 265, 266, 808
CseI GACGC 1 cut(s) 411
Csp6I GTAC 4 cut(s) 145, 214, 240, 435
CviAII CATG 5 cut(s) 262, 440, 451, 562, 862
CviQI GTAC 4 cut(s) 145, 214, 240, 435
DpnI GATC 5 cut(s) 5, 183, 293, 630, 872
DpnII GATC 5 cut(s) 3, 181, 291, 628, 870
DriI GACNNNNNGTC 1 cut(s) 717
Eam1105I GACNNNNNGTC 1 cut(s) 717
Eco130I CCWWGG 1 cut(s) 230
Eco24I GRGCYC 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 230
Eco88I CYCGRG 3 cut(s) 7, 283, 664
Eco91I GGTNACC 1 cut(s) 224
EcoNI CCTNNNNNAGG 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 266
EcoO65I GGTNACC 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 585
EcoRII CCWGG 3 cut(s) 295, 780, 804
EcoT14I CCWWGG 1 cut(s) 230
EcoT38I GRGCYC 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 230
FaeI CATG 5 cut(s) 265, 443, 454, 565, 865
FatI CATG 5 cut(s) 261, 439, 450, 561, 861
FauI CCCGC 2 cut(s) 34, 398
FbaI TGATCA 1 cut(s) 870
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 1 cut(s) 918
FokI GGATG 3 cut(s) 114, 443, 521
FriOI GRGCYC 1 cut(s) 269
Fsp4HI GCNGC 1 cut(s) 918
FspBI CTAG 2 cut(s) 231, 632
GlaI GCGC 1 cut(s) 697
GluI GCNGC 1 cut(s) 918
GsuI CTGGAG 1 cut(s) 137
HaeII RGCGCY 1 cut(s) 699
HaeIII GGCC 4 cut(s) 55, 60, 267, 810
HapII CCGG 4 cut(s) 49, 57, 159, 179
HgaI GACGC 1 cut(s) 411
HhaI GCGC 1 cut(s) 698
Hin1II CATG 5 cut(s) 265, 443, 454, 565, 865
Hin6I GCGC 1 cut(s) 696
HinP1I GCGC 1 cut(s) 696
HincII GTYRAC 2 cut(s) 70, 421
HindII GTYRAC 2 cut(s) 70, 421
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 2 cut(s) 483, 755
HpaI GTTAAC 1 cut(s) 70
HpaII CCGG 4 cut(s) 49, 57, 159, 179
HphI GGTGA 3 cut(s) 236, 522, 857
Hpy166II GTNNAC 5 cut(s) 70, 104, 242, 357, 421
Hpy188I TCNGA 3 cut(s) 30, 210, 401
Hpy188III TCNNGA 6 cut(s) 7, 283, 562, 673, 759, 874
Hpy8I GTNNAC 5 cut(s) 70, 104, 242, 357, 421
Hpy99I CGWCG 1 cut(s) 405
HpyAV CCTTC 3 cut(s) 35, 406, 781
HpyCH4IV ACGT 3 cut(s) 72, 134, 339
HpyCH4V TGCA 4 cut(s) 257, 792, 841, 891
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
HpySE526I ACGT 3 cut(s) 72, 134, 339
Hsp92II CATG 5 cut(s) 265, 443, 454, 565, 865
HspAI GCGC 1 cut(s) 696
KpnI GGTACC 1 cut(s) 438
Ksp22I TGATCA 1 cut(s) 870
KspAI GTTAAC 1 cut(s) 70
Kzo9I GATC 5 cut(s) 3, 181, 291, 628, 870
Lsp1109I GCAGC 1 cut(s) 904
MaeI CTAG 2 cut(s) 231, 632
MaeII ACGT 3 cut(s) 72, 134, 339
MaeIII GTNAC 4 cut(s) 224, 364, 827, 901
MalI GATC 5 cut(s) 5, 183, 293, 630, 872
MboI GATC 5 cut(s) 3, 181, 291, 628, 870
MboII GAAGA 2 cut(s) 713, 860
MflI RGATCY 2 cut(s) 181, 291
MhlI GDGCHC 1 cut(s) 269
MluCI AATT 5 cut(s) 466, 579, 585, 735, 740
MlyI GAGTC 2 cut(s) 492, 764
MmeI TCCRAC 2 cut(s) 53, 424
MseI TTAA 4 cut(s) 69, 342, 501, 522
MslI CAYNNNNRTG 1 cut(s) 866
MspA1I CMGCKG 1 cut(s) 917
MspI CCGG 4 cut(s) 49, 57, 159, 179
MspR9I CCNGG 5 cut(s) 57, 160, 297, 782, 806
Mva1269I GAATGC 1 cut(s) 862
MvaI CCWGG 3 cut(s) 297, 782, 806
MvnI CGCG 2 cut(s) 222, 405
MwoI GCNNNNNNNGC 1 cut(s) 61
NciI CCSGG 2 cut(s) 57, 160
NdeII GATC 5 cut(s) 3, 181, 291, 628, 870
NlaIII CATG 5 cut(s) 265, 443, 454, 565, 865
NlaIV GGNNCC 6 cut(s) 171, 183, 267, 268, 307, 436
NmuCI GTSAC 2 cut(s) 224, 364
PaeR7I CTCGAG 2 cut(s) 283, 664
PagI TCATGA 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 671
PctI GAATGC 1 cut(s) 862
PflMI CCANNNNNTGG 2 cut(s) 544, 920
PfoI TCCNGGA 1 cut(s) 158
PkrI GCNGC 1 cut(s) 919
PleI GAGTC 2 cut(s) 491, 763
PpsI GAGTC 2 cut(s) 491, 763
PshAI GACNNNNGTC 1 cut(s) 362
Psp1406I AACGTT 1 cut(s) 72
Psp6I CCWGG 3 cut(s) 295, 780, 804
PspEI GGTNACC 1 cut(s) 224
PspGI CCWGG 3 cut(s) 295, 780, 804
PspN4I GGNNCC 6 cut(s) 171, 183, 267, 268, 307, 436
PspOMI GGGCCC 1 cut(s) 265
PspPI GGNCC 4 cut(s) 54, 265, 266, 808
PspXI VCTCGAGB 1 cut(s) 664
PstI CTGCAG 1 cut(s) 843
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 2 cut(s) 181, 291
PvuII CAGCTG 1 cut(s) 917
RsaI GTAC 4 cut(s) 146, 215, 241, 436
RsaNI GTAC 4 cut(s) 145, 214, 240, 435
RseI CAYNNNNRTG 1 cut(s) 866
SalI GTCGAC 1 cut(s) 419
SaqAI TTAA 4 cut(s) 69, 342, 501, 522
SatI GCNGC 1 cut(s) 918
Sau3AI GATC 5 cut(s) 3, 181, 291, 628, 870
Sau96I GGNCC 4 cut(s) 54, 265, 266, 808
SchI GAGTC 2 cut(s) 492, 764
ScrFI CCNGG 5 cut(s) 57, 160, 297, 782, 806
SduI GDGCHC 1 cut(s) 269
SfcI CTRYAG 2 cut(s) 691, 839
Sfr274I CTCGAG 2 cut(s) 283, 664
SlaI CTCGAG 2 cut(s) 283, 664
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 2 cut(s) 283, 664
SmoI CTYRAG 2 cut(s) 283, 664
Sse9I AATT 5 cut(s) 466, 579, 585, 735, 740
SsiI CCGC 4 cut(s) 41, 222, 405, 533
SspMI CTAG 2 cut(s) 231, 632
StyD4I CCNGG 5 cut(s) 55, 158, 295, 780, 804
StyI CCWWGG 1 cut(s) 230
TaiI ACGT 3 cut(s) 75, 137, 342
TaqI TCGA 3 cut(s) 284, 420, 665
TasI AATT 5 cut(s) 466, 579, 585, 735, 740
TatI WGTACW 2 cut(s) 144, 213
Tru1I TTAA 4 cut(s) 69, 342, 501, 522
Tru9I TTAA 4 cut(s) 69, 342, 501, 522
TseFI GTSAC 2 cut(s) 224, 364
TseI GCWGC 1 cut(s) 917
Tsp45I GTSAC 2 cut(s) 224, 364
TspDTI ATGAA 3 cut(s) 116, 811, 878
TspGWI ACGGA 1 cut(s) 177
Van91I CCANNNNNTGG 2 cut(s) 544, 920
XagI CCTNNNNNAGG 1 cut(s) 300
XapI RAATTY 1 cut(s) 585
XhoI CTCGAG 2 cut(s) 283, 664
XmaJI CCTAGG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 420
XspI CTAG 2 cut(s) 231, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.