RNA binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 365, 680 |
| Acc36I | ACCTGC | 3 cut(s) | 365, 436, 680 |
| Acc65I | GGTACC | 1 cut(s) | 1304 |
| AccB1I | GGYRCC | 4 cut(s) | 1066, 1163, 1304, 1698 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 452, 1414 |
| AccI | GTMKAC | 2 cut(s) | 56, 1290 |
| AccII | CGCG | 1 cut(s) | 43 |
| AciI | CCGC | 6 cut(s) | 286, 601, 676, 810, 942, 1403 |
| AclI | AACGTT | 1 cut(s) | 969 |
| AclWI | GGATC | 5 cut(s) | 70, 75, 1073, 1086, 1183 |
| AcoI | YGGCCR | 1 cut(s) | 151 |
| AcsI | RAATTY | 1 cut(s) | 795 |
| AcuI | CTGAAG | 1 cut(s) | 124 |
| AfaI | GTAC | 4 cut(s) | 356, 533, 1138, 1306 |
| AfeI | AGCGCT | 2 cut(s) | 98, 402 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 295, 452, 1133, 1315, 1414 |
| AgeI | ACCGGT | 1 cut(s) | 311 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1587 |
| AjnI | CCWGG | 6 cut(s) | 153, 813, 1132, 1192, 1650, 1674 |
| AluBI | AGCT | 7 cut(s) | 164, 222, 449, 842, 1171, 1440, 1708 |
| AluI | AGCT | 7 cut(s) | 164, 222, 449, 842, 1171, 1440, 1708 |
| Alw21I | GWGCWC | 1 cut(s) | 844 |
| Alw26I | GTCTC | 5 cut(s) | 26, 260, 986, 995, 1183 |
| AlwI | GGATC | 5 cut(s) | 70, 75, 1073, 1086, 1183 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 452 |
| Ama87I | CYCGRG | 5 cut(s) | 257, 837, 908, 1180, 1534 |
| Aor51HI | AGCGCT | 2 cut(s) | 98, 402 |
| AoxI | GGCC | 6 cut(s) | 151, 811, 954, 1130, 1317, 1678 |
| ApeKI | GCWGC | 5 cut(s) | 103, 135, 164, 449, 1708 |
| ApoI | RAATTY | 1 cut(s) | 795 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 611, 643 |
| AsiGI | ACCGGT | 1 cut(s) | 311 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 513 |
| Asp718I | GGTACC | 1 cut(s) | 1304 |
| AspA2I | CCTAGG | 1 cut(s) | 1127 |
| AspLEI | GCGC | 4 cut(s) | 99, 327, 403, 686 |
| AspS9I | GGNCC | 3 cut(s) | 817, 955, 1678 |
| AsuHPI | GGTGA | 6 cut(s) | 389, 661, 816, 1133, 1392, 1486 |
| AvaI | CYCGRG | 5 cut(s) | 257, 837, 908, 1180, 1534 |
| AvaII | GGWCC | 1 cut(s) | 817 |
| AvrII | CCTAGG | 1 cut(s) | 1127 |
| BaeGI | GKGCMC | 1 cut(s) | 1166 |
| BalI | TGGCCA | 1 cut(s) | 153 |
| BamHI | GGATCC | 1 cut(s) | 1078 |
| BanI | GGYRCC | 4 cut(s) | 1066, 1163, 1304, 1698 |
| BanII | GRGCYC | 1 cut(s) | 844 |
| BbsI | GAAGAC | 1 cut(s) | 1583 |
| Bbv12I | GWGCWC | 1 cut(s) | 844 |
| BbvI | GCAGC | 5 cut(s) | 90, 122, 151, 436, 1695 |
| BccI | CCATC | 5 cut(s) | 106, 968, 1056, 1295, 1376 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 69, 103 |
| BciT130I | CCWGG | 6 cut(s) | 155, 815, 1134, 1194, 1652, 1676 |
| BclI | TGATCA | 1 cut(s) | 145 |
| BcoDI | GTCTC | 5 cut(s) | 26, 260, 986, 995, 1183 |
| BfaI | CTAG | 3 cut(s) | 1128, 1437, 1502 |
| BfmI | CTRYAG | 1 cut(s) | 1709 |
| BfoI | RGCGCY | 3 cut(s) | 100, 328, 404 |
| BfuAI | ACCTGC | 3 cut(s) | 365, 436, 680 |
| BisI | GCNGC | 7 cut(s) | 104, 136, 165, 450, 676, 811, 1709 |
| BlnI | CCTAGG | 1 cut(s) | 1127 |
| BlsI | GCNGC | 7 cut(s) | 105, 137, 166, 451, 677, 812, 1710 |
| Bme1390I | CCNGG | 6 cut(s) | 155, 815, 1134, 1194, 1652, 1676 |
| Bme18I | GGWCC | 1 cut(s) | 817 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1587 |
| BmeT110I | CYCGRG | 5 cut(s) | 257, 837, 908, 1180, 1534 |
| BmgT120I | GGNCC | 3 cut(s) | 817, 955, 1678 |
| BmiI | GGNNCC | 5 cut(s) | 1068, 1080, 1165, 1306, 1700 |
| BmrFI | CCNGG | 6 cut(s) | 155, 815, 1134, 1194, 1652, 1676 |
| BmsI | GCATC | 6 cut(s) | 106, 252, 295, 361, 764, 1701 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1257 |
| BpiI | GAAGAC | 1 cut(s) | 1583 |
| BpuEI | CTTGAG | 2 cut(s) | 427, 593 |
| BsaI | GGTCTC | 3 cut(s) | 26, 260, 1183 |
| BsaJI | CCNNGG | 5 cut(s) | 735, 761, 1127, 1192, 1675 |
| BsaWI | WCCGGW | 1 cut(s) | 311 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 554, 584, 826, 856 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 295, 452, 1133, 1315, 1414 |
| Bse118I | RCCGGY | 2 cut(s) | 311, 806 |
| Bse1I | ACTGG | 3 cut(s) | 199, 533, 820 |
| BseBI | CCWGG | 6 cut(s) | 155, 815, 1134, 1194, 1652, 1676 |
| BseDI | CCNNGG | 5 cut(s) | 735, 761, 1127, 1192, 1675 |
| BseGI | GGATG | 7 cut(s) | 121, 267, 310, 779, 1024, 1306, 1384 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 295, 452, 1133, 1315, 1414 |
| BseMII | CTCAG | 2 cut(s) | 219, 290 |
| BseNI | ACTGG | 3 cut(s) | 199, 533, 820 |
| BseSI | GKGCMC | 1 cut(s) | 1166 |
| BseXI | GCAGC | 5 cut(s) | 90, 122, 151, 436, 1695 |
| BseYI | CCCAGC | 2 cut(s) | 222, 327 |
| Bsh1236I | CGCG | 1 cut(s) | 43 |
| BshFI | GGCC | 6 cut(s) | 153, 813, 956, 1132, 1319, 1680 |
| BshNI | GGYRCC | 4 cut(s) | 1066, 1163, 1304, 1698 |
| BshTI | ACCGGT | 1 cut(s) | 311 |
| BsiHKAI | GWGCWC | 1 cut(s) | 844 |
| BsiHKCI | CYCGRG | 5 cut(s) | 257, 837, 908, 1180, 1534 |
| BsiSI | CCGG | 3 cut(s) | 312, 807, 1076 |
| BslFI | GGGAC | 1 cut(s) | 1568 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 295, 452, 1133, 1315, 1414 |
| BsmAI | GTCTC | 5 cut(s) | 26, 260, 986, 995, 1183 |
| BsmFI | GGGAC | 1 cut(s) | 1568 |
| BsnI | GGCC | 6 cut(s) | 153, 813, 956, 1132, 1319, 1680 |
| Bso31I | GGTCTC | 3 cut(s) | 26, 260, 1183 |
| BsoBI | CYCGRG | 5 cut(s) | 257, 837, 908, 1180, 1534 |
| Bsp1286I | GDGCHC | 2 cut(s) | 844, 1166 |
| Bsp143I | GATC | 8 cut(s) | 75, 80, 145, 234, 829, 1078, 1188, 1498 |
| BspACI | CCGC | 6 cut(s) | 286, 601, 676, 810, 942, 1403 |
| BspANI | GGCC | 6 cut(s) | 153, 813, 956, 1132, 1319, 1680 |
| BspCNI | CTCAG | 2 cut(s) | 220, 289 |
| BspFNI | CGCG | 1 cut(s) | 43 |
| BspHI | TCATGA | 1 cut(s) | 1431 |
| BspLI | GGNNCC | 5 cut(s) | 1068, 1080, 1165, 1306, 1700 |
| BspMAI | CTGCAG | 1 cut(s) | 1713 |
| BspMI | ACCTGC | 3 cut(s) | 365, 436, 680 |
| BspPI | GGATC | 5 cut(s) | 70, 75, 1073, 1086, 1183 |
| BspT107I | GGYRCC | 4 cut(s) | 1066, 1163, 1304, 1698 |
| BspTNI | GGTCTC | 3 cut(s) | 26, 260, 1183 |
| BsrFI | RCCGGY | 2 cut(s) | 311, 806 |
| BsrI | ACTGG | 3 cut(s) | 199, 533, 820 |
| BssAI | RCCGGY | 2 cut(s) | 311, 806 |
| BssECI | CCNNGG | 5 cut(s) | 735, 761, 1127, 1192, 1675 |
| BssMI | GATC | 8 cut(s) | 75, 80, 145, 234, 829, 1078, 1188, 1498 |
| BssT1I | CCWWGG | 2 cut(s) | 735, 1127 |
| Bst2UI | CCWGG | 6 cut(s) | 155, 815, 1134, 1194, 1652, 1676 |
| Bst4CI | ACNGT | 3 cut(s) | 172, 587, 1121 |
| Bst6I | CTCTTC | 1 cut(s) | 504 |
| BstC8I | GCNNGC | 3 cut(s) | 101, 720, 808 |
| BstDEI | CTNAG | 3 cut(s) | 27, 228, 276 |
| BstEII | GGTNACC | 1 cut(s) | 1121 |
| BstF5I | GGATG | 7 cut(s) | 121, 267, 310, 779, 1024, 1306, 1384 |
| BstFNI | CGCG | 1 cut(s) | 43 |
| BstH2I | RGCGCY | 3 cut(s) | 100, 328, 404 |
| BstHHI | GCGC | 4 cut(s) | 99, 327, 403, 686 |
| BstKTI | GATC | 8 cut(s) | 78, 83, 148, 237, 832, 1081, 1191, 1501 |
| BstMAI | GTCTC | 5 cut(s) | 26, 260, 986, 995, 1183 |
| BstMBI | GATC | 8 cut(s) | 75, 80, 145, 234, 829, 1078, 1188, 1498 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 96, 103, 398, 681, 690, 724, 1705 |
| BstNI | CCWGG | 6 cut(s) | 155, 815, 1134, 1194, 1652, 1676 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1257 |
| BstPI | GGTNACC | 1 cut(s) | 1121 |
| BstSCI | CCNGG | 6 cut(s) | 153, 813, 1132, 1192, 1650, 1674 |
| BstSFI | CTRYAG | 1 cut(s) | 1709 |
| BstSLI | GKGCMC | 1 cut(s) | 1166 |
| BstUI | CGCG | 1 cut(s) | 43 |
| BstV1I | GCAGC | 5 cut(s) | 90, 122, 151, 436, 1695 |
| BstV2I | GAAGAC | 1 cut(s) | 1583 |
| BstX2I | RGATCY | 3 cut(s) | 75, 1078, 1188 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1174 |
| BstYI | RGATCY | 3 cut(s) | 75, 1078, 1188 |
| BsuRI | GGCC | 6 cut(s) | 153, 813, 956, 1132, 1319, 1680 |
| BtgZI | GCGATG | 1 cut(s) | 1559 |
| BtsCI | GGATG | 7 cut(s) | 121, 267, 310, 779, 1024, 1306, 1384 |
| BtsIMutI | CAGTG | 3 cut(s) | 148, 168, 1103 |
| BveI | ACCTGC | 3 cut(s) | 365, 436, 680 |
| Cac8I | GCNNGC | 3 cut(s) | 101, 720, 808 |
| CaiI | CAGNNNCTG | 1 cut(s) | 452 |
| CciI | TCATGA | 1 cut(s) | 1431 |
| CfoI | GCGC | 4 cut(s) | 99, 327, 403, 686 |
| Cfr10I | RCCGGY | 2 cut(s) | 311, 806 |
| Cfr13I | GGNCC | 3 cut(s) | 817, 955, 1678 |
| CseI | GACGC | 2 cut(s) | 79, 1577 |
| Csp6I | GTAC | 4 cut(s) | 355, 532, 1137, 1305 |
| CspAI | ACCGGT | 1 cut(s) | 311 |
| CviAII | CATG | 9 cut(s) | 71, 149, 179, 630, 918, 1159, 1310, 1321, 1432 |
| CviQI | GTAC | 4 cut(s) | 355, 532, 1137, 1305 |
| DdeI | CTNAG | 3 cut(s) | 27, 228, 276 |
| DpnI | GATC | 8 cut(s) | 77, 82, 147, 236, 831, 1080, 1190, 1500 |
| DpnII | GATC | 8 cut(s) | 75, 80, 145, 234, 829, 1078, 1188, 1498 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1587 |
| EaeI | YGGCCR | 1 cut(s) | 151 |
| Eam1104I | CTCTTC | 1 cut(s) | 504 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1587 |
| EarI | CTCTTC | 1 cut(s) | 504 |
| Ecl136II | GAGCTC | 1 cut(s) | 842 |
| Eco130I | CCWWGG | 2 cut(s) | 735, 1127 |
| Eco147I | AGGCCT | 1 cut(s) | 1132 |
| Eco24I | GRGCYC | 1 cut(s) | 844 |
| Eco31I | GGTCTC | 3 cut(s) | 26, 260, 1183 |
| Eco47I | GGWCC | 1 cut(s) | 817 |
| Eco47III | AGCGCT | 2 cut(s) | 98, 402 |
| Eco53kI | GAGCTC | 1 cut(s) | 842 |
| Eco57I | CTGAAG | 1 cut(s) | 124 |
| Eco88I | CYCGRG | 5 cut(s) | 257, 837, 908, 1180, 1534 |
| Eco91I | GGTNACC | 1 cut(s) | 1121 |
| EcoICRI | GAGCTC | 1 cut(s) | 842 |
| EcoO109I | RGGNCCY | 1 cut(s) | 955 |
| EcoO65I | GGTNACC | 1 cut(s) | 1121 |
| EcoRII | CCWGG | 6 cut(s) | 153, 813, 1132, 1192, 1650, 1674 |
| EcoT14I | CCWWGG | 2 cut(s) | 735, 1127 |
| EcoT38I | GRGCYC | 1 cut(s) | 844 |
| ErhI | CCWWGG | 2 cut(s) | 735, 1127 |
| FaeI | CATG | 9 cut(s) | 74, 152, 182, 633, 921, 1162, 1313, 1324, 1435 |
| FaqI | GGGAC | 1 cut(s) | 1568 |
| FatI | CATG | 9 cut(s) | 70, 148, 178, 629, 917, 1158, 1309, 1320, 1431 |
| FauI | CCCGC | 1 cut(s) | 935 |
| FauNDI | CATATG | 1 cut(s) | 496 |
| FbaI | TGATCA | 1 cut(s) | 145 |
| FblI | GTMKAC | 2 cut(s) | 56, 1290 |
| Fnu4HI | GCNGC | 7 cut(s) | 104, 136, 165, 450, 676, 811, 1709 |
| FokI | GGATG | 7 cut(s) | 128, 274, 317, 786, 1011, 1313, 1391 |
| FriOI | GRGCYC | 1 cut(s) | 844 |
| Fsp4HI | GCNGC | 7 cut(s) | 104, 136, 165, 450, 676, 811, 1709 |
| FspBI | CTAG | 3 cut(s) | 1128, 1437, 1502 |
| GlaI | GCGC | 4 cut(s) | 98, 326, 402, 685 |
| GluI | GCNGC | 7 cut(s) | 104, 136, 165, 450, 676, 811, 1709 |
| GsaI | CCCAGC | 2 cut(s) | 226, 331 |
| HaeII | RGCGCY | 3 cut(s) | 100, 328, 404 |
| HaeIII | GGCC | 6 cut(s) | 153, 813, 956, 1132, 1319, 1680 |
| HapII | CCGG | 3 cut(s) | 312, 807, 1076 |
| HgaI | GACGC | 2 cut(s) | 79, 1577 |
| HhaI | GCGC | 4 cut(s) | 99, 327, 403, 686 |
| Hin1II | CATG | 9 cut(s) | 74, 152, 182, 633, 921, 1162, 1313, 1324, 1435 |
| Hin6I | GCGC | 4 cut(s) | 97, 325, 401, 684 |
| HinP1I | GCGC | 4 cut(s) | 97, 325, 401, 684 |
| HincII | GTYRAC | 3 cut(s) | 57, 967, 1291 |
| HindII | GTYRAC | 3 cut(s) | 57, 967, 1291 |
| HindIII | AAGCTT | 1 cut(s) | 1169 |
| HinfI | GANTC | 8 cut(s) | 14, 23, 38, 859, 1046, 1202, 1353, 1625 |
| HpaI | GTTAAC | 1 cut(s) | 967 |
| HpaII | CCGG | 3 cut(s) | 312, 807, 1076 |
| HphI | GGTGA | 6 cut(s) | 389, 661, 816, 1133, 1392, 1486 |
| Hpy166II | GTNNAC | 7 cut(s) | 57, 915, 967, 1001, 1139, 1252, 1291 |
| Hpy188I | TCNGA | 6 cut(s) | 22, 80, 219, 279, 762, 1201 |
| Hpy188III | TCNNGA | 9 cut(s) | 238, 259, 406, 473, 908, 1180, 1432, 1543, 1629 |
| Hpy8I | GTNNAC | 7 cut(s) | 57, 915, 967, 1001, 1139, 1252, 1291 |
| Hpy99I | CGWCG | 2 cut(s) | 95, 1562 |
| HpyAV | CCTTC | 2 cut(s) | 470, 1651 |
| HpyCH4III | ACNGT | 3 cut(s) | 172, 587, 1121 |
| HpyCH4IV | ACGT | 4 cut(s) | 594, 696, 969, 1233 |
| HpyCH4V | TGCA | 8 cut(s) | 374, 639, 693, 712, 732, 777, 1154, 1711 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 96, 103, 398, 681, 690, 724, 1705 |
| HpyF3I | CTNAG | 3 cut(s) | 27, 228, 276 |
| HpySE526I | ACGT | 4 cut(s) | 594, 696, 969, 1233 |
| Hsp92II | CATG | 9 cut(s) | 74, 152, 182, 633, 921, 1162, 1313, 1324, 1435 |
| HspAI | GCGC | 4 cut(s) | 97, 325, 401, 684 |
| KpnI | GGTACC | 1 cut(s) | 1308 |
| KroI | GCCGGC | 1 cut(s) | 806 |
| KroNI | GCCGGC | 1 cut(s) | 808 |
| Ksp22I | TGATCA | 1 cut(s) | 145 |
| KspAI | GTTAAC | 1 cut(s) | 967 |
| Kzo9I | GATC | 8 cut(s) | 75, 80, 145, 234, 829, 1078, 1188, 1498 |
| LmnI | GCTCC | 2 cut(s) | 219, 847 |
| Lsp1109I | GCAGC | 5 cut(s) | 90, 122, 151, 436, 1695 |
| LweI | GCATC | 6 cut(s) | 106, 252, 295, 361, 764, 1701 |
| MaeI | CTAG | 3 cut(s) | 1128, 1437, 1502 |
| MaeII | ACGT | 4 cut(s) | 594, 696, 969, 1233 |
| MaeIII | GTNAC | 4 cut(s) | 590, 822, 1121, 1259 |
| MalI | GATC | 8 cut(s) | 77, 82, 147, 236, 831, 1080, 1190, 1500 |
| MboI | GATC | 8 cut(s) | 75, 80, 145, 234, 829, 1078, 1188, 1498 |
| MboII | GAAGA | 4 cut(s) | 9, 392, 521, 1583 |
| MflI | RGATCY | 3 cut(s) | 75, 1078, 1188 |
| MhlI | GDGCHC | 2 cut(s) | 844, 1166 |
| MlsI | TGGCCA | 1 cut(s) | 153 |
| MluNI | TGGCCA | 1 cut(s) | 153 |
| MlyI | GAGTC | 2 cut(s) | 868, 1362 |
| MmeI | TCCRAC | 1 cut(s) | 87 |
| Mox20I | TGGCCA | 1 cut(s) | 153 |
| MreI | CGCCGGCG | 1 cut(s) | 806 |
| MroNI | GCCGGC | 1 cut(s) | 806 |
| MroXI | GAANNNNTTC | 1 cut(s) | 513 |
| MscI | TGGCCA | 1 cut(s) | 153 |
| MseI | TTAA | 8 cut(s) | 210, 366, 491, 966, 1053, 1371, 1392, 1729 |
| MslI | CAYNNNNRTG | 1 cut(s) | 183 |
| Msp20I | TGGCCA | 1 cut(s) | 153 |
| MspA1I | CMGCKG | 1 cut(s) | 449 |
| MspI | CCGG | 3 cut(s) | 312, 807, 1076 |
| MspR9I | CCNGG | 6 cut(s) | 155, 815, 1134, 1194, 1652, 1676 |
| MvaI | CCWGG | 6 cut(s) | 155, 815, 1134, 1194, 1652, 1676 |
| MvnI | CGCG | 1 cut(s) | 43 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 96, 103, 398, 681, 690, 724, 1705 |
| NaeI | GCCGGC | 1 cut(s) | 808 |
| NdeI | CATATG | 1 cut(s) | 496 |
| NdeII | GATC | 8 cut(s) | 75, 80, 145, 234, 829, 1078, 1188, 1498 |
| NgoMIV | GCCGGC | 1 cut(s) | 806 |
| NlaIII | CATG | 9 cut(s) | 74, 152, 182, 633, 921, 1162, 1313, 1324, 1435 |
| NlaIV | GGNNCC | 5 cut(s) | 1068, 1080, 1165, 1306, 1700 |
| NmeAIII | GCCGAG | 2 cut(s) | 882, 909 |
| NmuCI | GTSAC | 3 cut(s) | 822, 1121, 1259 |
| PaeR7I | CTCGAG | 3 cut(s) | 837, 1180, 1534 |
| PagI | TCATGA | 1 cut(s) | 1431 |
| PaqCI | CACCTGC | 2 cut(s) | 365, 680 |
| PceI | AGGCCT | 1 cut(s) | 1132 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 591, 1541 |
| PdiI | GCCGGC | 1 cut(s) | 808 |
| PdmI | GAANNNNTTC | 1 cut(s) | 513 |
| PfeI | GAWTC | 6 cut(s) | 14, 23, 38, 1046, 1202, 1625 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 452, 1414 |
| PinAI | ACCGGT | 1 cut(s) | 311 |
| PkrI | GCNGC | 7 cut(s) | 105, 137, 166, 451, 677, 812, 1710 |
| PleI | GAGTC | 2 cut(s) | 867, 1361 |
| PpsI | GAGTC | 2 cut(s) | 867, 1361 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1257 |
| Psp124BI | GAGCTC | 1 cut(s) | 844 |
| Psp1406I | AACGTT | 1 cut(s) | 969 |
| Psp6I | CCWGG | 6 cut(s) | 153, 813, 1132, 1192, 1650, 1674 |
| PspEI | GGTNACC | 1 cut(s) | 1121 |
| PspFI | CCCAGC | 2 cut(s) | 222, 327 |
| PspGI | CCWGG | 6 cut(s) | 153, 813, 1132, 1192, 1650, 1674 |
| PspN4I | GGNNCC | 5 cut(s) | 1068, 1080, 1165, 1306, 1700 |
| PspPI | GGNCC | 3 cut(s) | 817, 955, 1678 |
| PspXI | VCTCGAGB | 2 cut(s) | 837, 1534 |
| PstI | CTGCAG | 1 cut(s) | 1713 |
| PstNI | CAGNNNCTG | 1 cut(s) | 452 |
| PsuI | RGATCY | 3 cut(s) | 75, 1078, 1188 |
| PvuII | CAGCTG | 1 cut(s) | 449 |
| RsaI | GTAC | 4 cut(s) | 356, 533, 1138, 1306 |
| RsaNI | GTAC | 4 cut(s) | 355, 532, 1137, 1305 |
| RseI | CAYNNNNRTG | 1 cut(s) | 183 |
| SacI | GAGCTC | 1 cut(s) | 844 |
| SalI | GTCGAC | 2 cut(s) | 55, 1289 |
| SaqAI | TTAA | 8 cut(s) | 210, 366, 491, 966, 1053, 1371, 1392, 1729 |
| SatI | GCNGC | 7 cut(s) | 104, 136, 165, 450, 676, 811, 1709 |
| Sau3AI | GATC | 8 cut(s) | 75, 80, 145, 234, 829, 1078, 1188, 1498 |
| Sau96I | GGNCC | 3 cut(s) | 817, 955, 1678 |
| SchI | GAGTC | 2 cut(s) | 868, 1362 |
| ScrFI | CCNGG | 6 cut(s) | 155, 815, 1134, 1194, 1652, 1676 |
| SduI | GDGCHC | 2 cut(s) | 844, 1166 |
| SfaNI | GCATC | 6 cut(s) | 106, 252, 295, 361, 764, 1701 |
| SfcI | CTRYAG | 1 cut(s) | 1709 |
| Sfr274I | CTCGAG | 3 cut(s) | 837, 1180, 1534 |
| SgrAI | CRCCGGYG | 1 cut(s) | 806 |
| SinI | GGWCC | 1 cut(s) | 817 |
| SlaI | CTCGAG | 3 cut(s) | 837, 1180, 1534 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 183 |
| SmlI | CTYRAG | 5 cut(s) | 406, 608, 837, 1180, 1534 |
| SmoI | CTYRAG | 5 cut(s) | 406, 608, 837, 1180, 1534 |
| SseBI | AGGCCT | 1 cut(s) | 1132 |
| SsiI | CCGC | 6 cut(s) | 286, 601, 676, 810, 942, 1403 |
| SspI | AATATT | 1 cut(s) | 892 |
| SspMI | CTAG | 3 cut(s) | 1128, 1437, 1502 |
| SstI | GAGCTC | 1 cut(s) | 844 |
| StuI | AGGCCT | 1 cut(s) | 1132 |
| StyD4I | CCNGG | 6 cut(s) | 153, 813, 1132, 1192, 1650, 1674 |
| StyI | CCWWGG | 2 cut(s) | 735, 1127 |
| TaaI | ACNGT | 3 cut(s) | 172, 587, 1121 |
| TaiI | ACGT | 4 cut(s) | 597, 699, 972, 1236 |
| TaqI | TCGA | 8 cut(s) | 56, 93, 617, 838, 1044, 1181, 1290, 1535 |
| TaqII | GACCGA | 1 cut(s) | 50 |
| TauI | GCSGC | 2 cut(s) | 678, 813 |
| TfiI | GAWTC | 6 cut(s) | 14, 23, 38, 1046, 1202, 1625 |
| Tru1I | TTAA | 8 cut(s) | 210, 366, 491, 966, 1053, 1371, 1392, 1729 |
| Tru9I | TTAA | 8 cut(s) | 210, 366, 491, 966, 1053, 1371, 1392, 1729 |
| TscAI | CASTG | 3 cut(s) | 148, 175, 1110 |
| TseFI | GTSAC | 3 cut(s) | 822, 1121, 1259 |
| TseI | GCWGC | 5 cut(s) | 103, 135, 164, 449, 1708 |
| Tsp45I | GTSAC | 3 cut(s) | 822, 1121, 1259 |
| TspDTI | ATGAA | 2 cut(s) | 167, 453 |
| TspRI | CASTG | 3 cut(s) | 148, 175, 1110 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 452, 1414 |
| VpaK11BI | GGWCC | 1 cut(s) | 817 |
| XapI | RAATTY | 1 cut(s) | 795 |
| XhoI | CTCGAG | 3 cut(s) | 837, 1180, 1534 |
| XmaJI | CCTAGG | 1 cut(s) | 1127 |
| XmiI | GTMKAC | 2 cut(s) | 56, 1290 |
| XmnI | GAANNNNTTC | 1 cut(s) | 513 |
| XspI | CTAG | 3 cut(s) | 1128, 1437, 1502 |