Rh1AG449500

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000753
Physical Location & Seq
Forward (+)
66875274 .. 66888598
13325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG449500.1

Sequence Viewer

Length: 924 bp
ATGGAAATTCCGAGTCATATGGGTTGCGCTCCTTATTCTGGCGGAATCATGTTCGCCGGCGGCCTGGACCAATCACCAGATCAAACTCAAGCTCCTTACTTTGTGCCGAGTGGAAAATGTTTTTTTTTTAAGCCGGGAGATATTTCCAACGATTATCCCGAGTGGATAAGGCTTCCTTCCGATATCAAAAGCAGGAAACCGGAGGCCCTGCTGGTTAACGTTGATGGAAGTCTCTATTGTCTCTCTGGTTTACTCATTTATCAGCCTGTCGTTCATCCTACGTTTGAGGTGTACAATCGCAATTACGAGAGATGGGTGCCTCTGCCGGATCCTCCTTTTTACAGACCCGAAGCACTGAATTACAAACACGGTGACCCTAGGCCTGGTACACACTTGTCTTATGCAATCATGGGCACCAAGCTTTTGGTCTCGAGCAAGATACCTGGTACAGGTTCCTGCCCTCACTTTCCCGTTTGTTGCTTTGACGTTAAGGAAAAAAAGTGGACAGAGGTCACTACCTTGTTCGATGGTAAGCCTTTTCCCTTCCGACTCGGGGCTTTAGTGGTCGACTTGGAGGATGGTACCCATGATAAGGTCATGTTTTACTTCAATAATTATTTTGCTATAAGAGCCTGCCGATTAGTAGTTAATGAGGATGATGGTAGTATTAAGAACTCACCCCCCCAATCTTTGGGGATCGATTCCCTCATGACTAGCTTATTGGAAATAACCATTCGAAATCAGTCAATGGTAAGAATGTCGTGGGATTTTGTTGATCTAGGAAATAAAAAAGTCTGCTTTGTTTTGTCCTCGAGCCTCCCGAAAGTCAGAGACGACTATAGCGCCACTCATCGCCAGACGATTCGCCTTCTTGTCTTGATGCAGCCCTGGGCGCCAAAGTATTCTCAAAGACTGCCAGAGTAA

Protein Analysis

307

Amino Acids

34.76

Weight (kDa)

7.03

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 57 - 184 1.6e-08 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 581
AccB1I GGYRCC 4 cut(s) 316, 413, 581, 892
AccB7I CCANNNNNTGG 1 cut(s) 691
AccI GTMKAC 1 cut(s) 567
AciI CCGC 2 cut(s) 42, 60
AclI AACGTT 1 cut(s) 219
AclWI GGATC 3 cut(s) 323, 336, 704
AcsI RAATTY 1 cut(s) 6
AcyI GRCGYC 1 cut(s) 893
AfaI GTAC 4 cut(s) 293, 388, 448, 583
AfiI CCNNNNNNNGG 6 cut(s) 38, 383, 449, 553, 592, 691
AgsI TTSAA 1 cut(s) 610
AjnI CCWGG 4 cut(s) 63, 382, 442, 887
AluBI AGCT 3 cut(s) 92, 421, 717
AluI AGCT 3 cut(s) 92, 421, 717
Alw26I GTCTC 4 cut(s) 236, 245, 433, 825
AlwI GGATC 3 cut(s) 323, 336, 704
Ama87I CYCGRG 4 cut(s) 158, 430, 551, 811
AoxI GGCC 3 cut(s) 61, 204, 380
ApeKI GCWGC 1 cut(s) 883
ApoI RAATTY 1 cut(s) 6
Asp718I GGTACC 1 cut(s) 581
AspA2I CCTAGG 1 cut(s) 377
AspLEI GCGC 3 cut(s) 29, 845, 895
AspS9I GGNCC 2 cut(s) 67, 205
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 3 cut(s) 66, 383, 669
AsuII TTCGAA 1 cut(s) 736
AvaI CYCGRG 4 cut(s) 158, 430, 551, 811
AvaII GGWCC 1 cut(s) 67
AvrII CCTAGG 1 cut(s) 377
BaeGI GKGCMC 1 cut(s) 416
BamHI GGATCC 1 cut(s) 328
BanI GGYRCC 4 cut(s) 316, 413, 581, 892
BbvI GCAGC 1 cut(s) 895
BccI CCATC 5 cut(s) 218, 306, 521, 572, 653
BciT130I CCWGG 4 cut(s) 65, 384, 444, 889
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 4 cut(s) 236, 245, 433, 825
BfaI CTAG 3 cut(s) 378, 714, 779
BfmI CTRYAG 1 cut(s) 838
BfoI RGCGCY 2 cut(s) 846, 896
BisI GCNGC 2 cut(s) 61, 884
BlnI CCTAGG 1 cut(s) 377
BlsI GCNGC 2 cut(s) 62, 885
Bme1390I CCNGG 5 cut(s) 65, 135, 384, 444, 889
Bme18I GGWCC 1 cut(s) 67
BmeT110I CYCGRG 4 cut(s) 158, 430, 551, 811
BmgT120I GGNCC 2 cut(s) 67, 205
BmiI GGNNCC 6 cut(s) 318, 330, 415, 454, 583, 894
BmrFI CCNGG 5 cut(s) 65, 135, 384, 444, 889
BmsI GCATC 1 cut(s) 870
BoxI GACNNNNGTC 1 cut(s) 509
Bpu14I TTCGAA 1 cut(s) 736
BpuEI CTTGAG 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 135
Bsa29I ATCGAT 1 cut(s) 699
BsaHI GRCGYC 1 cut(s) 893
BsaI GGTCTC 1 cut(s) 433
BsaJI CCNNGG 3 cut(s) 377, 887, 888
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 2 cut(s) 76, 106
Bsc4I CCNNNNNNNGG 6 cut(s) 38, 383, 449, 553, 592, 691
Bse118I RCCGGY 1 cut(s) 56
BseBI CCWGG 4 cut(s) 65, 384, 444, 889
BseCI ATCGAT 1 cut(s) 699
BseDI CCNNGG 3 cut(s) 377, 887, 888
BseGI GGATG 3 cut(s) 274, 583, 661
BseLI CCNNNNNNNGG 6 cut(s) 38, 383, 449, 553, 592, 691
BseSI GKGCMC 1 cut(s) 416
BseXI GCAGC 1 cut(s) 895
BshFI GGCC 3 cut(s) 63, 206, 382
BshNI GGYRCC 4 cut(s) 316, 413, 581, 892
BshVI ATCGAT 1 cut(s) 699
BsiHKCI CYCGRG 4 cut(s) 158, 430, 551, 811
BsiSI CCGG 4 cut(s) 57, 134, 200, 326
BslI CCNNNNNNNGG 6 cut(s) 38, 383, 449, 553, 592, 691
BsmAI GTCTC 4 cut(s) 236, 245, 433, 825
BsmBI CGTCTC 1 cut(s) 825
BsnI GGCC 3 cut(s) 63, 206, 382
Bso31I GGTCTC 1 cut(s) 433
BsoBI CYCGRG 4 cut(s) 158, 430, 551, 811
Bsp119I TTCGAA 1 cut(s) 736
Bsp1286I GDGCHC 1 cut(s) 416
Bsp1407I TGTACA 1 cut(s) 291
Bsp143I GATC 4 cut(s) 79, 328, 696, 775
BspACI CCGC 2 cut(s) 42, 60
BspANI GGCC 3 cut(s) 63, 206, 382
BspDI ATCGAT 1 cut(s) 699
BspHI TCATGA 1 cut(s) 708
BspLI GGNNCC 6 cut(s) 318, 330, 415, 454, 583, 894
BspPI GGATC 3 cut(s) 323, 336, 704
BspT104I TTCGAA 1 cut(s) 736
BspT107I GGYRCC 4 cut(s) 316, 413, 581, 892
BspTNI GGTCTC 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 56
BsrGI TGTACA 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 3 cut(s) 377, 887, 888
BssMI GATC 4 cut(s) 79, 328, 696, 775
BssNI GRCGYC 1 cut(s) 893
BssT1I CCWWGG 1 cut(s) 377
Bst2UI CCWGG 4 cut(s) 65, 384, 444, 889
Bst4CI ACNGT 1 cut(s) 371
BstACI GRCGYC 1 cut(s) 893
BstAUI TGTACA 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 736
BstC8I GCNNGC 2 cut(s) 58, 634
BstEII GGTNACC 1 cut(s) 371
BstENI CCTNNNNNAGG 1 cut(s) 447
BstF5I GGATG 3 cut(s) 274, 583, 661
BstH2I RGCGCY 2 cut(s) 846, 896
BstHHI GCGC 3 cut(s) 29, 845, 895
BstKTI GATC 4 cut(s) 82, 331, 699, 778
BstMAI GTCTC 4 cut(s) 236, 245, 433, 825
BstMBI GATC 4 cut(s) 79, 328, 696, 775
BstMWI GCNNNNNNNGC 2 cut(s) 629, 892
BstNI CCWGG 4 cut(s) 65, 384, 444, 889
BstPAI GACNNNNGTC 1 cut(s) 509
BstPI GGTNACC 1 cut(s) 371
BstSCI CCNGG 5 cut(s) 63, 133, 382, 442, 887
BstSFI CTRYAG 1 cut(s) 838
BstSLI GKGCMC 1 cut(s) 416
BstV1I GCAGC 1 cut(s) 895
BstX2I RGATCY 1 cut(s) 328
BstXI CCANNNNNNTGG 1 cut(s) 424
BstYI RGATCY 1 cut(s) 328
Bsu15I ATCGAT 1 cut(s) 699
BsuRI GGCC 3 cut(s) 63, 206, 382
BsuTUI ATCGAT 1 cut(s) 699
BtgZI GCGATG 1 cut(s) 836
BtsCI GGATG 3 cut(s) 274, 583, 661
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 2 cut(s) 58, 634
CciI TCATGA 1 cut(s) 708
CfoI GCGC 3 cut(s) 29, 845, 895
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 2 cut(s) 67, 205
ClaI ATCGAT 1 cut(s) 699
CsiI ACCWGGT 1 cut(s) 442
Csp6I GTAC 4 cut(s) 292, 387, 447, 582
CviAII CATG 5 cut(s) 49, 409, 587, 598, 709
CviQI GTAC 4 cut(s) 292, 387, 447, 582
DinI GGCGCC 1 cut(s) 894
DpnI GATC 4 cut(s) 81, 330, 698, 777
DpnII GATC 4 cut(s) 79, 328, 696, 775
EciI GGCGGA 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 377
Eco147I AGGCCT 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 433
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 67
Eco88I CYCGRG 4 cut(s) 158, 430, 551, 811
Eco91I GGTNACC 1 cut(s) 371
EcoNI CCTNNNNNAGG 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 205
EcoO65I GGTNACC 1 cut(s) 371
EcoRII CCWGG 4 cut(s) 63, 382, 442, 887
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 377
EgeI GGCGCC 1 cut(s) 894
EheI GGCGCC 1 cut(s) 894
ErhI CCWWGG 1 cut(s) 377
Esp3I CGTCTC 1 cut(s) 825
FaeI CATG 5 cut(s) 52, 412, 590, 601, 712
FalI AAGNNNNNCTT 2 cut(s) 160, 192
FatI CATG 5 cut(s) 48, 408, 586, 597, 708
FauNDI CATATG 1 cut(s) 18
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 2 cut(s) 61, 884
FokI GGATG 3 cut(s) 261, 590, 668
Fsp4HI GCNGC 2 cut(s) 61, 884
FspBI CTAG 3 cut(s) 378, 714, 779
GlaI GCGC 3 cut(s) 28, 844, 894
GluI GCNGC 2 cut(s) 61, 884
HaeII RGCGCY 2 cut(s) 846, 896
HaeIII GGCC 3 cut(s) 63, 206, 382
HapII CCGG 4 cut(s) 57, 134, 200, 326
HhaI GCGC 3 cut(s) 29, 845, 895
Hin1I GRCGYC 1 cut(s) 893
Hin1II CATG 5 cut(s) 52, 412, 590, 601, 712
Hin6I GCGC 3 cut(s) 27, 843, 893
HinP1I GCGC 3 cut(s) 27, 843, 893
HincII GTYRAC 2 cut(s) 217, 568
HindII GTYRAC 2 cut(s) 217, 568
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 5 cut(s) 13, 45, 549, 701, 862
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 4 cut(s) 57, 134, 200, 326
HphI GGTGA 3 cut(s) 66, 383, 669
Hpy166II GTNNAC 6 cut(s) 217, 251, 292, 389, 504, 568
Hpy188I TCNGA 4 cut(s) 12, 181, 548, 830
Hpy188III TCNNGA 5 cut(s) 158, 430, 709, 820, 877
Hpy8I GTNNAC 6 cut(s) 217, 251, 292, 389, 504, 568
HpyAV CCTTC 3 cut(s) 186, 553, 878
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4IV ACGT 3 cut(s) 219, 281, 486
HpyCH4V TGCA 2 cut(s) 404, 883
HpyF10VI GCNNNNNNNGC 2 cut(s) 629, 892
HpySE526I ACGT 3 cut(s) 219, 281, 486
Hsp92I GRCGYC 1 cut(s) 893
Hsp92II CATG 5 cut(s) 52, 412, 590, 601, 712
HspAI GCGC 3 cut(s) 27, 843, 893
KasI GGCGCC 1 cut(s) 892
KpnI GGTACC 1 cut(s) 585
KroI GCCGGC 1 cut(s) 56
KroNI GCCGGC 1 cut(s) 58
KspAI GTTAAC 1 cut(s) 217
Kzo9I GATC 4 cut(s) 79, 328, 696, 775
LmnI GCTCC 2 cut(s) 34, 97
Lsp1109I GCAGC 1 cut(s) 895
LweI GCATC 1 cut(s) 870
MabI ACCWGGT 1 cut(s) 442
MaeI CTAG 3 cut(s) 378, 714, 779
MaeII ACGT 3 cut(s) 219, 281, 486
MaeIII GTNAC 2 cut(s) 371, 511
MalI GATC 4 cut(s) 81, 330, 698, 777
MboI GATC 4 cut(s) 79, 328, 696, 775
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 1 cut(s) 416
MluCI AATT 4 cut(s) 6, 301, 358, 613
Mly113I GGCGCC 1 cut(s) 893
MlyI GAGTC 2 cut(s) 22, 543
MmeI TCCRAC 2 cut(s) 171, 571
MreI CGCCGGCG 1 cut(s) 56
MroNI GCCGGC 1 cut(s) 56
MseI TTAA 5 cut(s) 129, 216, 489, 648, 669
MspI CCGG 4 cut(s) 57, 134, 200, 326
MspR9I CCNGG 5 cut(s) 65, 135, 384, 444, 889
MvaI CCWGG 4 cut(s) 65, 384, 444, 889
MwoI GCNNNNNNNGC 2 cut(s) 629, 892
NaeI GCCGGC 1 cut(s) 58
NarI GGCGCC 1 cut(s) 893
NciI CCSGG 1 cut(s) 135
NdeI CATATG 1 cut(s) 18
NdeII GATC 4 cut(s) 79, 328, 696, 775
NgoMIV GCCGGC 1 cut(s) 56
NlaIII CATG 5 cut(s) 52, 412, 590, 601, 712
NlaIV GGNNCC 6 cut(s) 318, 330, 415, 454, 583, 894
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 371, 511
NspV TTCGAA 1 cut(s) 736
PaeR7I CTCGAG 2 cut(s) 430, 811
PagI TCATGA 1 cut(s) 708
PasI CCCWGGG 1 cut(s) 888
PceI AGGCCT 1 cut(s) 382
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 818
PdiI GCCGGC 1 cut(s) 58
PfeI GAWTC 3 cut(s) 45, 701, 862
PflMI CCANNNNNTGG 1 cut(s) 691
PkrI GCNGC 2 cut(s) 62, 885
PleI GAGTC 2 cut(s) 21, 543
PluTI GGCGCC 1 cut(s) 896
PpsI GAGTC 2 cut(s) 21, 543
PshAI GACNNNNGTC 1 cut(s) 509
Psp1406I AACGTT 1 cut(s) 219
Psp6I CCWGG 4 cut(s) 63, 382, 442, 887
PspEI GGTNACC 1 cut(s) 371
PspGI CCWGG 4 cut(s) 63, 382, 442, 887
PspN4I GGNNCC 6 cut(s) 318, 330, 415, 454, 583, 894
PspPI GGNCC 2 cut(s) 67, 205
PspXI VCTCGAGB 1 cut(s) 811
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 4 cut(s) 293, 388, 448, 583
RsaNI GTAC 4 cut(s) 292, 387, 447, 582
SalI GTCGAC 1 cut(s) 566
SaqAI TTAA 5 cut(s) 129, 216, 489, 648, 669
SatI GCNGC 2 cut(s) 61, 884
Sau3AI GATC 4 cut(s) 79, 328, 696, 775
Sau96I GGNCC 2 cut(s) 67, 205
SchI GAGTC 2 cut(s) 22, 543
ScrFI CCNGG 5 cut(s) 65, 135, 384, 444, 889
SduI GDGCHC 1 cut(s) 416
SexAI ACCWGGT 1 cut(s) 442
SfaNI GCATC 1 cut(s) 870
SfcI CTRYAG 1 cut(s) 838
SfoI GGCGCC 1 cut(s) 894
Sfr274I CTCGAG 2 cut(s) 430, 811
SfuI TTCGAA 1 cut(s) 736
SgrAI CRCCGGYG 1 cut(s) 56
SinI GGWCC 1 cut(s) 67
SlaI CTCGAG 2 cut(s) 430, 811
SmlI CTYRAG 3 cut(s) 87, 430, 811
SmoI CTYRAG 3 cut(s) 87, 430, 811
Sse9I AATT 4 cut(s) 6, 301, 358, 613
SseBI AGGCCT 1 cut(s) 382
SsiI CCGC 2 cut(s) 42, 60
SspDI GGCGCC 1 cut(s) 892
SspMI CTAG 3 cut(s) 378, 714, 779
StuI AGGCCT 1 cut(s) 382
StyD4I CCNGG 5 cut(s) 63, 133, 382, 442, 887
StyI CCWWGG 1 cut(s) 377
TaaI ACNGT 1 cut(s) 371
TaiI ACGT 3 cut(s) 222, 284, 489
TaqI TCGA 6 cut(s) 431, 525, 567, 699, 736, 812
TasI AATT 4 cut(s) 6, 301, 358, 613
TatI WGTACW 1 cut(s) 291
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 3 cut(s) 45, 701, 862
Tru1I TTAA 5 cut(s) 129, 216, 489, 648, 669
Tru9I TTAA 5 cut(s) 129, 216, 489, 648, 669
TscAI CASTG 1 cut(s) 360
TseFI GTSAC 2 cut(s) 371, 511
TseI GCWGC 1 cut(s) 883
Tsp45I GTSAC 2 cut(s) 371, 511
TspDTI ATGAA 1 cut(s) 263
TspRI CASTG 1 cut(s) 360
Van91I CCANNNNNTGG 1 cut(s) 691
VpaK11BI GGWCC 1 cut(s) 67
XagI CCTNNNNNAGG 1 cut(s) 447
XapI RAATTY 1 cut(s) 6
XhoI CTCGAG 2 cut(s) 430, 811
XmaJI CCTAGG 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 567
XspI CTAG 3 cut(s) 378, 714, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.