MD01G1065600.v1.1

Belongs to the cysteine synthase cystathionine beta- synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001137
Physical Location & Seq
Forward (+)
16936127 .. 16941633
5507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1065600.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGCTGCCCTAAGGAGCTTTCTCAAGAAAAGATCCTCTGTGCTTTGCAATGGAGGAGCAGTGAGACACAGGCTCTTCTCCGCTCAGGTGTCCCAACCCATCCCCGATTCTCCATCTTTCGCTCAGAGAATCGGAAATCTCCCCAAGGATCTTCCTGCCACCCAAATCAAAACCCAAGTTTCCCAACTCATTGGTAGAACTCCCATTGTTTATCTTAACAAAGTCACTGAGGGATGTGGAGCTTTCATTGCTGTCAAGCAAGAGATGTTCCAACCCACCGCTAGCATCAAGGACAGACCGGCACTTTCTATGATCAACGATGCAGAAGAGAAGGGCTTGATAACTCCCGGGGAGACAACGCTGATCGAGCCAACGTCGGGGAATATGGGAATCAGCATGGCTTTTATGGCGGCCATGAAAGGGTATAAGATGGTTCTAACTTTGCCGTCTTACACTAGTTTGGAGAGAAGGGTGTGTATGAGATGCTTTGGAGCTGATTTAATTCTCACTGATCCAACCAAGGGGATGGGAGGGACTGTTAAGAAGGCGTATGATCTTTTGGAATCTACGCCGAATGCCTTTATGCTCCAACAATTCTCAAATCCCGCCAATACTAAGGTGCATTTTGAAACTACAGGCCCTGAGATATGGGAGGATACCAACGGACAAGTTGACATCTTCGTAATGGGAATTGGTAGCGGAGGCACTGTCTCTGGGGTCGGGCAGTATCTCAAATCTAAAAATCCTAATGTTCAGATATACGGAGTGGAGCCTGCTGAAAGTAATGTGCTGAATGGTGGTAAACCAGGTCCTCATTCAATCACGGGCAACGGGGTTGGATTCAAACCAGATATATTGGACATGGATATGATGGAAAGAGTGATTGAGGTTCGTAGCGAAGATGCAGTAAACATGGCAAGACAATTGGCACTGAAAGAAGGGCTTATGGTGGGAATATCGTCTGGAGCCAACACAGTGGCAGCAATGGAGCTTGCTAAAAAGCCTGAAAACAAAGGCAAACTCATTGTGACTGTTCACGCGAGCTTTGGGGAGCGATACTTGTCATCTGTTCTATTTCAAGACCTGAGGCAGGAAGCCGAAAACATGCAACCAGTTGCAGTCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0000097 GO:0002376 GO:0003674 GO:0003824 GO:0004124 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006520 GO:0006534 GO:0006535 GO:0006563 GO:0006790 GO:0006807 GO:0006955 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0009056 GO:0009058 GO:0009069 GO:0009070 GO:0009507 GO:0009509 GO:0009532 GO:0009536 GO:0009570 GO:0009636 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016053 GO:0016740 GO:0016765 GO:0016829 GO:0016846 GO:0019344 GO:0019499 GO:0019500 GO:0019752 GO:0019842 GO:0021700 GO:0022622 GO:0030154 GO:0030170 GO:0032501 GO:0032502 GO:0034641 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046872 GO:0046914 GO:0048037 GO:0048364 GO:0048468 GO:0048469 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050017 GO:0050662 GO:0050896 GO:0051410 GO:0070279 GO:0071695 GO:0071704 GO:0080147 GO:0090558 GO:0090627 GO:0097159 GO:0098754 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901605 GO:1901607 GO:1901698 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00270 ko00460 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00460 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

376

Amino Acids

40.47

Weight (kDa)

7.63

Isoelectric Point (pI)

32.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 60 - 346 1.8e-72 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 1122
AccII CGCG 1 cut(s) 1040
AciI CCGC 5 cut(s) 81, 279, 410, 606, 699
AclWI GGATC 3 cut(s) 27, 156, 506
AcoI YGGCCR 1 cut(s) 411
AfiI CCNNNNNNNGG 4 cut(s) 377, 420, 521, 1090
AgsI TTSAA 4 cut(s) 629, 819, 844, 1079
AhlI ACTAGT 1 cut(s) 455
AjnI CCWGG 1 cut(s) 805
AluBI AGCT 5 cut(s) 18, 242, 494, 991, 1044
AluI AGCT 5 cut(s) 18, 242, 494, 991, 1044
Alw26I GTCTC 3 cut(s) 58, 347, 715
AlwI GGATC 3 cut(s) 27, 156, 506
AlwNI CAGNNNCTG 1 cut(s) 641
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 2 cut(s) 411, 637
ApeKI GCWGC 2 cut(s) 5, 980
AspS9I GGNCC 2 cut(s) 638, 809
AsuC2I CCSGG 2 cut(s) 348, 349
AsuNHI GCTAGC 1 cut(s) 281
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 1 cut(s) 809
AxyI CCTNAGG 2 cut(s) 11, 1085
BbvI GCAGC 1 cut(s) 992
BccI CCATC 5 cut(s) 107, 121, 424, 520, 865
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 1 cut(s) 807
BciVI GTATCC 1 cut(s) 649
BclI TGATCA 1 cut(s) 312
BcnI CCSGG 2 cut(s) 348, 349
BcoDI GTCTC 3 cut(s) 58, 347, 715
BcuI ACTAGT 1 cut(s) 455
BfaI CTAG 2 cut(s) 282, 456
BfmI CTRYAG 1 cut(s) 633
BfuI GTATCC 1 cut(s) 649
BisI GCNGC 3 cut(s) 6, 411, 981
BlsI GCNGC 3 cut(s) 7, 412, 982
Bme1390I CCNGG 3 cut(s) 348, 349, 807
Bme18I GGWCC 1 cut(s) 809
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 2 cut(s) 638, 809
BmiI GGNNCC 2 cut(s) 771, 967
BmrFI CCNGG 3 cut(s) 348, 349, 807
BmsI GCATC 4 cut(s) 294, 310, 473, 892
BmtI GCTAGC 1 cut(s) 285
BpmI CTGGAG 1 cut(s) 984
Bpu10I CCTNAGC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 8
BpuMI CCSGG 2 cut(s) 348, 349
BsaJI CCNNGG 4 cut(s) 144, 347, 348, 519
BsaXI ACNNNNNCTCC 6 cut(s) 45, 75, 522, 552, 693, 723
Bsc4I CCNNNNNNNGG 4 cut(s) 377, 420, 521, 1090
Bse118I RCCGGY 1 cut(s) 298
Bse1I ACTGG 1 cut(s) 1112
Bse21I CCTNAGG 2 cut(s) 11, 1085
Bse3DI GCAATG 3 cut(s) 55, 246, 990
BseBI CCWGG 1 cut(s) 807
BseDI CCNNGG 4 cut(s) 144, 347, 348, 519
BseGI GGATG 3 cut(s) 99, 239, 531
BseLI CCNNNNNNNGG 4 cut(s) 377, 420, 521, 1090
BseMI GCAATG 3 cut(s) 55, 246, 990
BseMII CTCAG 5 cut(s) 98, 137, 219, 633, 1076
BseNI ACTGG 1 cut(s) 1112
BseRI GAGGAG 1 cut(s) 69
BseXI GCAGC 1 cut(s) 992
Bsh1236I CGCG 1 cut(s) 1040
BshFI GGCC 2 cut(s) 413, 639
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 2 cut(s) 299, 348
BslFI GGGAC 2 cut(s) 76, 547
BslI CCNNNNNNNGG 4 cut(s) 377, 420, 521, 1090
BsmAI GTCTC 3 cut(s) 58, 347, 715
BsmFI GGGAC 2 cut(s) 76, 547
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 2 cut(s) 413, 639
BsoBI CYCGRG 1 cut(s) 347
Bsp143I GATC 6 cut(s) 32, 148, 312, 363, 511, 553
BspACI CCGC 5 cut(s) 81, 279, 410, 606, 699
BspANI GGCC 2 cut(s) 413, 639
BspCNI CTCAG 5 cut(s) 97, 136, 220, 634, 1077
BspFNI CGCG 1 cut(s) 1040
BspLI GGNNCC 2 cut(s) 771, 967
BspOI GCTAGC 1 cut(s) 285
BspPI GGATC 3 cut(s) 27, 156, 506
BspQI GCTCTTC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 83
BsrDI GCAATG 3 cut(s) 55, 246, 990
BsrFI RCCGGY 1 cut(s) 298
BsrI ACTGG 1 cut(s) 1112
BssAI RCCGGY 1 cut(s) 298
BssECI CCNNGG 4 cut(s) 144, 347, 348, 519
BssMI GATC 6 cut(s) 32, 148, 312, 363, 511, 553
BssT1I CCWWGG 2 cut(s) 144, 519
Bst2UI CCWGG 1 cut(s) 807
Bst4CI ACNGT 4 cut(s) 538, 709, 976, 1033
Bst6I CTCTTC 2 cut(s) 80, 321
BstC8I GCNNGC 4 cut(s) 283, 774, 993, 1042
BstDEI CTNAG 7 cut(s) 11, 84, 123, 228, 615, 642, 1085
BstENI CCTNNNNNAGG 1 cut(s) 1088
BstF5I GGATG 3 cut(s) 99, 239, 531
BstFNI CGCG 1 cut(s) 1040
BstKTI GATC 6 cut(s) 35, 151, 315, 366, 514, 556
BstMAI GTCTC 3 cut(s) 58, 347, 715
BstMBI GATC 6 cut(s) 32, 148, 312, 363, 511, 553
BstMWI GCNNNNNNNGC 3 cut(s) 248, 367, 407
BstNI CCWGG 1 cut(s) 807
BstNSI RCATGY 1 cut(s) 1108
BstSCI CCNGG 3 cut(s) 346, 347, 805
BstSFI CTRYAG 1 cut(s) 633
BstUI CGCG 1 cut(s) 1040
BstV1I GCAGC 1 cut(s) 992
BstX2I RGATCY 2 cut(s) 32, 148
BstXI CCANNNNNNTGG 3 cut(s) 191, 526, 976
BstYI RGATCY 2 cut(s) 32, 148
Bsu36I CCTNAGG 2 cut(s) 11, 1085
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 2 cut(s) 413, 639
BtsCI GGATG 3 cut(s) 99, 239, 531
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 6 cut(s) 66, 225, 507, 705, 929, 981
Cac8I GCNNGC 4 cut(s) 283, 774, 993, 1042
CaiI CAGNNNCTG 1 cut(s) 641
Cfr10I RCCGGY 1 cut(s) 298
Cfr13I GGNCC 2 cut(s) 638, 809
Cfr9I CCCGGG 1 cut(s) 347
CsiI ACCWGGT 1 cut(s) 805
CviAII CATG 5 cut(s) 397, 415, 862, 913, 1105
DdeI CTNAG 7 cut(s) 11, 84, 123, 228, 615, 642, 1085
DpnI GATC 6 cut(s) 34, 150, 314, 365, 513, 555
DpnII GATC 6 cut(s) 32, 148, 312, 363, 511, 553
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 2 cut(s) 80, 321
EarI CTCTTC 2 cut(s) 80, 321
Eco130I CCWWGG 2 cut(s) 144, 519
Eco47I GGWCC 1 cut(s) 809
Eco81I CCTNAGG 2 cut(s) 11, 1085
Eco88I CYCGRG 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 1088
EcoO109I RGGNCCY 2 cut(s) 638, 809
EcoRII CCWGG 1 cut(s) 805
EcoT14I CCWWGG 2 cut(s) 144, 519
ErhI CCWWGG 2 cut(s) 144, 519
FaeI CATG 5 cut(s) 400, 418, 865, 916, 1108
FalI AAGNNNNNCTT 2 cut(s) 927, 959
FaqI GGGAC 2 cut(s) 76, 547
FatI CATG 5 cut(s) 396, 414, 861, 912, 1104
FauI CCCGC 1 cut(s) 613
FbaI TGATCA 1 cut(s) 312
FblI GTMKAC 1 cut(s) 1122
Fnu4HI GCNGC 3 cut(s) 6, 411, 981
FokI GGATG 3 cut(s) 86, 246, 538
Fsp4HI GCNGC 3 cut(s) 6, 411, 981
FspBI CTAG 2 cut(s) 282, 456
GluI GCNGC 3 cut(s) 6, 411, 981
GsuI CTGGAG 1 cut(s) 984
HaeIII GGCC 2 cut(s) 413, 639
HapII CCGG 2 cut(s) 299, 348
Hin1II CATG 5 cut(s) 400, 418, 865, 916, 1108
HincII GTYRAC 2 cut(s) 673, 1123
HindII GTYRAC 2 cut(s) 673, 1123
HinfI GANTC 5 cut(s) 107, 129, 390, 563, 840
HpaII CCGG 2 cut(s) 299, 348
Hpy166II GTNNAC 5 cut(s) 673, 803, 910, 1036, 1123
Hpy188I TCNGA 3 cut(s) 126, 134, 756
Hpy188III TCNNGA 3 cut(s) 25, 963, 1079
Hpy8I GTNNAC 5 cut(s) 673, 803, 910, 1036, 1123
Hpy99I CGWCG 1 cut(s) 379
HpyAV CCTTC 4 cut(s) 325, 462, 538, 932
HpyCH4III ACNGT 4 cut(s) 538, 709, 976, 1033
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 6 cut(s) 48, 323, 622, 905, 1108, 1118
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 367, 407
HpyF3I CTNAG 7 cut(s) 11, 84, 123, 228, 615, 642, 1085
HpySE526I ACGT 1 cut(s) 374
Hsp92II CATG 5 cut(s) 400, 418, 865, 916, 1108
Ksp22I TGATCA 1 cut(s) 312
Kzo9I GATC 6 cut(s) 32, 148, 312, 363, 511, 553
LguI GCTCTTC 1 cut(s) 80
LmnI GCTCC 9 cut(s) 15, 56, 239, 491, 591, 769, 965, 988, 1051
Lsp1109I GCAGC 1 cut(s) 992
LweI GCATC 4 cut(s) 294, 310, 473, 892
MabI ACCWGGT 1 cut(s) 805
MaeI CTAG 2 cut(s) 282, 456
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 2 cut(s) 223, 1027
MalI GATC 6 cut(s) 34, 150, 314, 365, 513, 555
MbiI CCGCTC 1 cut(s) 83
MboI GATC 6 cut(s) 32, 148, 312, 363, 511, 553
MboII GAAGA 5 cut(s) 67, 143, 338, 670, 911
MfeI CAATTG 1 cut(s) 923
MflI RGATCY 2 cut(s) 32, 148
MluCI AATT 4 cut(s) 501, 593, 690, 923
MmeI TCCRAC 4 cut(s) 295, 539, 613, 817
MnlI CCTC 9 cut(s) 46, 47, 223, 524, 646, 695, 822, 880, 1080
MseI TTAA 3 cut(s) 216, 500, 540
MslI CAYNNNNRTG 1 cut(s) 866
MspI CCGG 2 cut(s) 299, 348
MspR9I CCNGG 3 cut(s) 348, 349, 807
MunI CAATTG 1 cut(s) 923
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 1 cut(s) 807
MvnI CGCG 1 cut(s) 1040
MwoI GCNNNNNNNGC 3 cut(s) 248, 367, 407
NciI CCSGG 2 cut(s) 348, 349
NdeII GATC 6 cut(s) 32, 148, 312, 363, 511, 553
NheI GCTAGC 1 cut(s) 281
NlaIII CATG 5 cut(s) 400, 418, 865, 916, 1108
NlaIV GGNNCC 2 cut(s) 771, 967
NmuCI GTSAC 2 cut(s) 223, 1027
NspI RCATGY 1 cut(s) 1108
PciSI GCTCTTC 1 cut(s) 80
PctI GAATGC 1 cut(s) 580
PfeI GAWTC 5 cut(s) 107, 129, 390, 563, 840
PkrI GCNGC 3 cut(s) 7, 412, 982
PpuMI RGGWCCY 1 cut(s) 809
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 1 cut(s) 805
PspGI CCWGG 1 cut(s) 805
PspN4I GGNNCC 2 cut(s) 771, 967
PspPI GGNCC 2 cut(s) 638, 809
PspPPI RGGWCCY 1 cut(s) 809
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 2 cut(s) 32, 148
RseI CAYNNNNRTG 1 cut(s) 866
SalI GTCGAC 1 cut(s) 1121
SapI GCTCTTC 1 cut(s) 80
SaqAI TTAA 3 cut(s) 216, 500, 540
SatI GCNGC 3 cut(s) 6, 411, 981
Sau3AI GATC 6 cut(s) 32, 148, 312, 363, 511, 553
Sau96I GGNCC 2 cut(s) 638, 809
ScrFI CCNGG 3 cut(s) 348, 349, 807
SexAI ACCWGGT 1 cut(s) 805
SfaNI GCATC 4 cut(s) 294, 310, 473, 892
SfcI CTRYAG 1 cut(s) 633
SinI GGWCC 1 cut(s) 809
SmaI CCCGGG 1 cut(s) 349
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
SpeI ACTAGT 1 cut(s) 455
Sse9I AATT 4 cut(s) 501, 593, 690, 923
SsiI CCGC 5 cut(s) 81, 279, 410, 606, 699
SspMI CTAG 2 cut(s) 282, 456
StyD4I CCNGG 3 cut(s) 346, 347, 805
StyI CCWWGG 2 cut(s) 144, 519
TaaI ACNGT 4 cut(s) 538, 709, 976, 1033
TaiI ACGT 1 cut(s) 377
TaqI TCGA 2 cut(s) 366, 1122
TasI AATT 4 cut(s) 501, 593, 690, 923
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 5 cut(s) 107, 129, 390, 563, 840
Tru1I TTAA 3 cut(s) 216, 500, 540
Tru9I TTAA 3 cut(s) 216, 500, 540
TscAI CASTG 6 cut(s) 66, 232, 514, 712, 936, 981
TseFI GTSAC 2 cut(s) 223, 1027
TseI GCWGC 2 cut(s) 5, 980
Tsp45I GTSAC 2 cut(s) 223, 1027
TspDTI ATGAA 2 cut(s) 235, 431
TspGWI ACGGA 2 cut(s) 678, 777
TspMI CCCGGG 1 cut(s) 347
TspRI CASTG 6 cut(s) 66, 232, 514, 712, 936, 981
VpaK11BI GGWCC 1 cut(s) 809
XagI CCTNNNNNAGG 1 cut(s) 1088
XceI RCATGY 1 cut(s) 1108
XmaI CCCGGG 1 cut(s) 347
XmiI GTMKAC 1 cut(s) 1122
XspI CTAG 2 cut(s) 282, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.