MD07G1135000.v1.1

Belongs to the cysteine synthase cystathionine beta- synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001137
Physical Location & Seq
Reverse (-)
19692242 .. 19696017
3776 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1135000.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGGCGGCTTTAAGGAGCTTTCTGAAGAAAAGGTCCTCTGTGCTCTGCAATGGAGCCATGAGACGGAAACTGTTCTCCACCGAGGTGTCCCAACCCCTCACTGATTCTCCATCTTTTGCTCAGAGAATCAGAAATCTCCCCAAGGATCTTCCTGGCACCCAAATCAAAACCCAAGTTTCCCAACTCATTGGTAGAACTCCTATTGTTTATCTTAACAAAGTCACTGAGGGATGTGGAGCGTATATTGCCGTCAAGCAAGAGATGTTCCAACCCACCGCCAGCATCAAGGACAGACCGGCACTTTCTATGATCAACGATGCAGAAGAGAAGGGCTTGATAACTCCTGGGGAGACAATACTGATCGAGCCAACATCAGGAAATATGGGAATCAGCATGGCGTTTATGGCGGCCATGAGAGGGTATAAAATGGTTTTAACCATGCCATCTTACACAAGCTTGGAGAGAAGGGTGTGTATGAGATGCTTTGGAGCTGATTTGATTCTCACTGATCCAACCAAGGGGATGGGAGGAACTGTTAAGAAGGCGTATGATCTTCTGGAATCCACGCCCAATGCCTATATGCTGCAACAATTCTCAAATCCCGCCAATACTAAGGCATCAATTTTTTTTTATCTATCATTATGCTATGTACATTTTGAAACTACAGGCCCTGAGATATGGGAGGATACCAACGGACAAGTTGACATCTTCGTAATGGGAATCGGTAGTGGAGGCACTGTCTCTGGAGTCGGGCAGTATCTCAAATCTAAAAATCCTAATGTTCAGATATATGGAGTGGAGCCTGCTGAAAGTAATGTGCTGAATGGCGGTAAACCAGGTCCTCATTCAATCATGGGCAACGGGGTTGGGTTCAAACCAGATATATTGGACTTGGATATGTTGGAAAGAGTTATTGAGGTTACCAGTGAAGATGCAGTAAACATGGCAAGACAATTGGCATTGAAAGAAGGGCTTATGGTTGGAATATCGTCTGGAGCCAACACTGTGGCAGCAATGGAGCTTGCTAAAAAGCCAGAAAACAAAGGCAAACTCATTGTGACTGTTCACCCGAGCTTTGGGGAGCGATACTTGTCATCCGTTCTATTTCAAGAGCTGAGGCAGGAAGCCGAAAACATGCAACCAGTTGCCGTCGACTATCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0000097 GO:0002376 GO:0003674 GO:0003824 GO:0004124 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006520 GO:0006534 GO:0006535 GO:0006563 GO:0006790 GO:0006807 GO:0006955 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0009056 GO:0009058 GO:0009069 GO:0009070 GO:0009507 GO:0009509 GO:0009532 GO:0009536 GO:0009570 GO:0009636 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016053 GO:0016740 GO:0016765 GO:0016829 GO:0016846 GO:0019344 GO:0019499 GO:0019500 GO:0019752 GO:0019842 GO:0021700 GO:0022622 GO:0030154 GO:0030170 GO:0032501 GO:0032502 GO:0034641 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046872 GO:0046914 GO:0048037 GO:0048364 GO:0048468 GO:0048469 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050017 GO:0050662 GO:0050896 GO:0051410 GO:0070279 GO:0071695 GO:0071704 GO:0080147 GO:0090558 GO:0090627 GO:0097159 GO:0098754 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901605 GO:1901607 GO:1901698 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00270 ko00460 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00460 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

388

Amino Acids

42.19

Weight (kDa)

7.57

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 59 - 357 1.4e-69 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 155
AccI GTMKAC 1 cut(s) 1152
AciI CCGC 5 cut(s) 5, 276, 407, 603, 828
AclWI GGATC 2 cut(s) 153, 503
AcoI YGGCCR 1 cut(s) 408
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 1 cut(s) 651
AfiI CCNNNNNNNGG 5 cut(s) 63, 374, 417, 518, 1076
AgsI TTSAA 5 cut(s) 659, 849, 874, 964, 1109
AjnI CCWGG 3 cut(s) 151, 343, 835
AleI CACNNNNGTG 1 cut(s) 83
AluBI AGCT 6 cut(s) 18, 456, 491, 1021, 1074, 1114
AluI AGCT 6 cut(s) 18, 456, 491, 1021, 1074, 1114
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 3 cut(s) 55, 344, 745
AlwI GGATC 2 cut(s) 153, 503
AlwNI CAGNNNCTG 1 cut(s) 671
Ama87I CYCGRG 1 cut(s) 1069
AoxI GGCC 2 cut(s) 408, 667
ApeKI GCWGC 2 cut(s) 583, 1010
Asp700I GAANNNNTTC 1 cut(s) 71
AspS9I GGNCC 3 cut(s) 33, 668, 839
AsuHPI GGTGA 1 cut(s) 1058
AvaI CYCGRG 1 cut(s) 1069
AvaII GGWCC 2 cut(s) 33, 839
BanI GGYRCC 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 45
BbvCI CCTCAGC 1 cut(s) 1115
BbvI GCAGC 2 cut(s) 570, 1022
BccI CCATC 3 cut(s) 118, 451, 517
BceAI ACGGC 2 cut(s) 233, 1133
BciT130I CCWGG 3 cut(s) 153, 345, 837
BciVI GTATCC 1 cut(s) 679
BclI TGATCA 1 cut(s) 309
BcoDI GTCTC 3 cut(s) 55, 344, 745
BfmI CTRYAG 1 cut(s) 663
BfuI GTATCC 1 cut(s) 679
BisI GCNGC 4 cut(s) 6, 408, 584, 1011
BlsI GCNGC 4 cut(s) 7, 409, 585, 1012
Bme1390I CCNGG 3 cut(s) 153, 345, 837
Bme18I GGWCC 2 cut(s) 33, 839
BmeT110I CYCGRG 1 cut(s) 1069
BmgT120I GGNCC 3 cut(s) 33, 668, 839
BmiI GGNNCC 4 cut(s) 55, 157, 801, 997
BmrFI CCNGG 3 cut(s) 153, 345, 837
BmsI GCATC 5 cut(s) 291, 307, 470, 626, 922
BpmI CTGGAG 2 cut(s) 765, 1014
Bpu10I CCTNAGC 1 cut(s) 1115
BsaJI CCNNGG 4 cut(s) 81, 141, 344, 516
BsaXI ACNNNNNCTCC 6 cut(s) 91, 121, 519, 549, 723, 753
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 374, 417, 518, 1076
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 2 cut(s) 924, 1142
Bse3DI GCAATG 2 cut(s) 55, 1020
BseBI CCWGG 3 cut(s) 153, 345, 837
BseDI CCNNGG 4 cut(s) 81, 141, 344, 516
BseGI GGATG 3 cut(s) 236, 528, 1094
BseLI CCNNNNNNNGG 5 cut(s) 63, 374, 417, 518, 1076
BseMI GCAATG 2 cut(s) 55, 1020
BseMII CTCAG 4 cut(s) 134, 216, 663, 1106
BseNI ACTGG 2 cut(s) 924, 1142
BseXI GCAGC 2 cut(s) 570, 1022
BshFI GGCC 2 cut(s) 410, 669
BshNI GGYRCC 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 1069
BsiSI CCGG 1 cut(s) 296
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 5 cut(s) 63, 374, 417, 518, 1076
BsmAI GTCTC 3 cut(s) 55, 344, 745
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 1 cut(s) 73
BsnI GGCC 2 cut(s) 410, 669
BsoBI CYCGRG 1 cut(s) 1069
Bsp1286I GDGCHC 1 cut(s) 45
Bsp1407I TGTACA 1 cut(s) 649
Bsp143I GATC 5 cut(s) 145, 309, 360, 508, 550
BspACI CCGC 5 cut(s) 5, 276, 407, 603, 828
BspANI GGCC 2 cut(s) 410, 669
BspCNI CTCAG 4 cut(s) 133, 217, 664, 1107
BspLI GGNNCC 4 cut(s) 55, 157, 801, 997
BspPI GGATC 2 cut(s) 153, 503
BspT107I GGYRCC 1 cut(s) 155
BsrDI GCAATG 2 cut(s) 55, 1020
BsrFI RCCGGY 1 cut(s) 295
BsrGI TGTACA 1 cut(s) 649
BsrI ACTGG 2 cut(s) 924, 1142
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 4 cut(s) 81, 141, 344, 516
BssMI GATC 5 cut(s) 145, 309, 360, 508, 550
BssT1I CCWWGG 2 cut(s) 141, 516
Bst2UI CCWGG 3 cut(s) 153, 345, 837
Bst4CI ACNGT 5 cut(s) 72, 535, 739, 1006, 1063
Bst6I CTCTTC 1 cut(s) 318
BstAUI TGTACA 1 cut(s) 649
BstC8I GCNNGC 3 cut(s) 280, 804, 1023
BstDEI CTNAG 5 cut(s) 120, 225, 612, 672, 1115
BstEII GGTNACC 1 cut(s) 919
BstF5I GGATG 3 cut(s) 236, 528, 1094
BstKTI GATC 5 cut(s) 148, 312, 363, 511, 553
BstMAI GTCTC 3 cut(s) 55, 344, 745
BstMBI GATC 5 cut(s) 145, 309, 360, 508, 550
BstMWI GCNNNNNNNGC 2 cut(s) 245, 404
BstNI CCWGG 3 cut(s) 153, 345, 837
BstNSI RCATGY 1 cut(s) 1138
BstPI GGTNACC 1 cut(s) 919
BstSCI CCNGG 3 cut(s) 151, 343, 835
BstSFI CTRYAG 1 cut(s) 663
BstV1I GCAGC 2 cut(s) 570, 1022
BstX2I RGATCY 1 cut(s) 145
BstXI CCANNNNNNTGG 3 cut(s) 188, 523, 1006
BstYI RGATCY 1 cut(s) 145
BsuI GTATCC 1 cut(s) 679
BsuRI GGCC 2 cut(s) 410, 669
BtsCI GGATG 3 cut(s) 236, 528, 1094
BtsIMutI CAGTG 6 cut(s) 99, 222, 504, 735, 931, 1002
Cac8I GCNNGC 3 cut(s) 280, 804, 1023
CaiI CAGNNNCTG 1 cut(s) 671
Cfr10I RCCGGY 1 cut(s) 295
Cfr13I GGNCC 3 cut(s) 33, 668, 839
CsiI ACCWGGT 1 cut(s) 835
Csp6I GTAC 1 cut(s) 650
CviAII CATG 7 cut(s) 58, 394, 412, 439, 853, 943, 1135
CviQI GTAC 1 cut(s) 650
DdeI CTNAG 5 cut(s) 120, 225, 612, 672, 1115
DpnI GATC 5 cut(s) 147, 311, 362, 510, 552
DpnII GATC 5 cut(s) 145, 309, 360, 508, 550
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Eco130I CCWWGG 2 cut(s) 141, 516
Eco47I GGWCC 2 cut(s) 33, 839
Eco57I CTGAAG 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 1069
Eco91I GGTNACC 1 cut(s) 919
EcoO109I RGGNCCY 3 cut(s) 33, 668, 839
EcoO65I GGTNACC 1 cut(s) 919
EcoRII CCWGG 3 cut(s) 151, 343, 835
EcoT14I CCWWGG 2 cut(s) 141, 516
ErhI CCWWGG 2 cut(s) 141, 516
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 7 cut(s) 61, 397, 415, 442, 856, 946, 1138
FalI AAGNNNNNCTT 2 cut(s) 957, 989
FaqI GGGAC 1 cut(s) 73
FatI CATG 7 cut(s) 57, 393, 411, 438, 852, 942, 1134
FauI CCCGC 1 cut(s) 610
FbaI TGATCA 1 cut(s) 309
FblI GTMKAC 1 cut(s) 1152
Fnu4HI GCNGC 4 cut(s) 6, 408, 584, 1011
FokI GGATG 3 cut(s) 243, 535, 1081
Fsp4HI GCNGC 4 cut(s) 6, 408, 584, 1011
GluI GCNGC 4 cut(s) 6, 408, 584, 1011
GsuI CTGGAG 2 cut(s) 765, 1014
HaeIII GGCC 2 cut(s) 410, 669
HapII CCGG 1 cut(s) 296
Hin1II CATG 7 cut(s) 61, 397, 415, 442, 856, 946, 1138
HincII GTYRAC 2 cut(s) 703, 1153
HindII GTYRAC 2 cut(s) 703, 1153
HindIII AAGCTT 1 cut(s) 454
HinfI GANTC 7 cut(s) 104, 126, 387, 499, 560, 720, 747
HpaII CCGG 1 cut(s) 296
HphI GGTGA 1 cut(s) 1058
Hpy166II GTNNAC 5 cut(s) 703, 833, 940, 1066, 1153
Hpy188I TCNGA 4 cut(s) 24, 123, 131, 786
Hpy188III TCNNGA 5 cut(s) 375, 557, 744, 993, 1109
Hpy8I GTNNAC 5 cut(s) 703, 833, 940, 1066, 1153
Hpy99I CGWCG 1 cut(s) 1154
HpyAV CCTTC 4 cut(s) 322, 459, 535, 962
HpyCH4III ACNGT 5 cut(s) 72, 535, 739, 1006, 1063
HpyCH4V TGCA 5 cut(s) 48, 320, 586, 935, 1138
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 404
HpyF3I CTNAG 5 cut(s) 120, 225, 612, 672, 1115
Hsp92II CATG 7 cut(s) 61, 397, 415, 442, 856, 946, 1138
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 5 cut(s) 145, 309, 360, 508, 550
LmnI GCTCC 8 cut(s) 15, 53, 236, 488, 799, 995, 1018, 1081
Lsp1109I GCAGC 2 cut(s) 570, 1022
LweI GCATC 5 cut(s) 291, 307, 470, 626, 922
MabI ACCWGGT 1 cut(s) 835
MaeIII GTNAC 3 cut(s) 220, 919, 1057
MalI GATC 5 cut(s) 147, 311, 362, 510, 552
MboI GATC 5 cut(s) 145, 309, 360, 508, 550
MboII GAAGA 6 cut(s) 37, 140, 335, 545, 700, 941
MfeI CAATTG 1 cut(s) 953
MflI RGATCY 1 cut(s) 145
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 3 cut(s) 590, 621, 953
MlyI GAGTC 1 cut(s) 756
MmeI TCCRAC 4 cut(s) 292, 536, 882, 961
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 4 cut(s) 11, 213, 434, 537
MslI CAYNNNNRTG 1 cut(s) 83
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 3 cut(s) 153, 345, 837
MunI CAATTG 1 cut(s) 953
MvaI CCWGG 3 cut(s) 153, 345, 837
MwoI GCNNNNNNNGC 2 cut(s) 245, 404
NdeII GATC 5 cut(s) 145, 309, 360, 508, 550
NlaIII CATG 7 cut(s) 61, 397, 415, 442, 856, 946, 1138
NlaIV GGNNCC 4 cut(s) 55, 157, 801, 997
NmuCI GTSAC 2 cut(s) 220, 1057
NspI RCATGY 1 cut(s) 1138
OliI CACNNNNGTG 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 6 cut(s) 104, 126, 387, 499, 560, 720
PkrI GCNGC 4 cut(s) 7, 409, 585, 1012
PleI GAGTC 1 cut(s) 755
PpsI GAGTC 1 cut(s) 755
PpuMI RGGWCCY 2 cut(s) 33, 839
Psp5II RGGWCCY 2 cut(s) 33, 839
Psp6I CCWGG 3 cut(s) 151, 343, 835
PspEI GGTNACC 1 cut(s) 919
PspGI CCWGG 3 cut(s) 151, 343, 835
PspN4I GGNNCC 4 cut(s) 55, 157, 801, 997
PspPI GGNCC 3 cut(s) 33, 668, 839
PspPPI RGGWCCY 2 cut(s) 33, 839
PstNI CAGNNNCTG 1 cut(s) 671
PsuI RGATCY 1 cut(s) 145
RsaI GTAC 1 cut(s) 651
RsaNI GTAC 1 cut(s) 650
RseI CAYNNNNRTG 1 cut(s) 83
SalI GTCGAC 1 cut(s) 1151
SaqAI TTAA 4 cut(s) 11, 213, 434, 537
SatI GCNGC 4 cut(s) 6, 408, 584, 1011
Sau3AI GATC 5 cut(s) 145, 309, 360, 508, 550
Sau96I GGNCC 3 cut(s) 33, 668, 839
SchI GAGTC 1 cut(s) 756
ScrFI CCNGG 3 cut(s) 153, 345, 837
SduI GDGCHC 1 cut(s) 45
SexAI ACCWGGT 1 cut(s) 835
SfaNI GCATC 5 cut(s) 291, 307, 470, 626, 922
SfcI CTRYAG 1 cut(s) 663
SinI GGWCC 2 cut(s) 33, 839
SmiMI CAYNNNNRTG 1 cut(s) 83
Sse9I AATT 3 cut(s) 590, 621, 953
SsiI CCGC 5 cut(s) 5, 276, 407, 603, 828
StyD4I CCNGG 3 cut(s) 151, 343, 835
StyI CCWWGG 2 cut(s) 141, 516
TaaI ACNGT 5 cut(s) 72, 535, 739, 1006, 1063
TaqI TCGA 2 cut(s) 363, 1152
TasI AATT 3 cut(s) 590, 621, 953
TatI WGTACW 1 cut(s) 649
TauI GCSGC 2 cut(s) 8, 410
TfiI GAWTC 6 cut(s) 104, 126, 387, 499, 560, 720
Tru1I TTAA 4 cut(s) 11, 213, 434, 537
Tru9I TTAA 4 cut(s) 11, 213, 434, 537
TscAI CASTG 6 cut(s) 106, 229, 511, 742, 931, 1009
TseFI GTSAC 2 cut(s) 220, 1057
TseI GCWGC 2 cut(s) 583, 1010
Tsp45I GTSAC 2 cut(s) 220, 1057
TspGWI ACGGA 3 cut(s) 79, 708, 1087
TspRI CASTG 6 cut(s) 106, 229, 511, 742, 931, 1009
VpaK11BI GGWCC 2 cut(s) 33, 839
XceI RCATGY 1 cut(s) 1138
XmiI GTMKAC 1 cut(s) 1152
XmnI GAANNNNTTC 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.