MD07G1072300.v1.1

GABA transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000597
Physical Location & Seq
Forward (+)
6833186 .. 6834458
1273 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1072300.v1.1.491

Sequence Viewer

Length: 900 bp
ATGCAACTCTACCAATTTGTAATTATATTTGGTGTCGTGATGCTGGTGTTGGCACAAATTCCATCCTTCCACTCCTTGAGGCATATCAACCTTATCTCTCTAATCCTTTGTCTTGCATACAGCATCTGTGCCACAGCTGGTTCCGTATACGTTGGAAATTCGGCGAATGCTCCTAGGAAGGACTACTCTTTAAATGGAAGTGGACAGAATCGTGTTTTTGGATCCTTCAATGCTATTTCGATCATCGCTACCTCGTATGGAAATGGCATTATTCCTGAAATACAGGCAACAATAGCTCCTCCAGTTAAAGGAAAAATGTTCAAAGGACTATGCGTATGCTATGCTGTTGTAATTTCGACATTTTTCAGTGTTGGTATCTCGGGTTATTGGGCGTTCGGAAATCAGGCCGAAGGAACAGTTCTGCTCAATTTTTTAGTTGACGGAAAGCCTTTGTTGCCAACTTGGGTTCTCTTGATGACCAATGTTTTCACCTTCTTGCAAGTATCAGCTGTTAGTGTGGTTAATTTACAAGTTACAAATGAAGTACTTGAACGCAAGTTTGTGAACACAAAGGTTGATCAATTCTCTGTTCGCAATGTAGTGCCAAGGTTGGTTTATCGATCTCTGTCCGTGGTCATAGCCACAACTGTTGCAGCTATGTTTCCATTCTTTGGCGACATCAACGCTTTGATTGGAGCATTTGGATGCATTCCTCTGGACTTCGTTTTGCCAACGGTGTTCTACAATGTTGTGTTCAAGCCCTCCAAGTACAGCTTACTTTTCTGGGGAAACACAATCGTTGCCTTAACATTTTCTGCGCTAGGACTGTTGGGTGCAATATCTTCGATCCGACAGATCGTTCTTGATTCTAACACATACAGTTTTTTTGCTAACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

300

Amino Acids

32.55

Weight (kDa)

9.22

Isoelectric Point (pI)

16.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 2 - 287 2.7e-40 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000597)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08230 AT1G08230 AT1G08230 AT1G08230 AT1G08230
fragaria_vesca FvH4_3g30140 FvH4_7g06340 FvH4_7g06350 FvH4_7g06350
malus_domestica MD02G1244800.v1.1 MD03G1133300.v1.1 MD07G1072100.v1.1 MD07G1072300.v1.1 MD11G1155800.v1.1 MD11G1156000.v1.1
prunus_persica Prupe.2G090300_v2.0.a1 Prupe.2G090500_v2.0.a1 Prupe.6G118000_v2.0.a1 Prupe.6G118000_v2.0.a1 Prupe.6G118000_v2.0.a1
pyrus_communis pycom02g21070 pycom03g08970 pycom07g05520
rosa_chinensis RchiOBHm_Chr1g0334311 RchiOBHm_Chr1g0334341 RchiOBHm_Chr1g0334351 RchiOBHm_Chr1g0334371 RchiOBHm_Chr1g0334381 RchiOBHm_Chr1g0334431 RchiOBHm_Chr5g0055491 RchiOBHm_Chr5g0055511
rosa_laevigata RLG00000029454 RLG00000029457 RLG00000029458 RLG00000029459 RLG00000029461 RLG00000034991 RLG00000034993
rosa_multiflora Rmu_sc0001673.1_g000036 Rmu_sc0001673.1_g000040 Rmu_sc0016880.1_g000002 Rmu_ssc0000047.1_g000014 Rmu_ssc0000047.1_g000015 Rmu_ssc0000047.1_g000016 Rmu_ssc0000047.1_g000023
rosa_roxburghii Rroxscaffold_1G00024820 Rroxscaffold_1G00024850 Rroxscaffold_1G00024860 Rroxscaffold_4G00316880 Rroxscaffold_4G00316900
rosa_rugosa Rorug01G0113300.1 Rorug01G0113400 Rorug01G0113500 Rorug05G0293200 Rorug05G0293200 Rorug05G0293400.1
rosa_samantha Rh1AG138200 Rh1AG138300 Rh1AG139000 Rh1BG104900 Rh1CG130400 Rh1CG130500 Rh1CG131000 Rh1DG142700 Rh1DG142800 Rh5AG362800 Rh5AG363000 Rh5BG375000 Rh5BG375200 Rh5CG396900 Rh5CG397100 Rh5DG388300 Rh5DG388400
rosa_wichuraiana Rw0G003720 Rw0G003730 Rw0G003740 Rw1G011440 Rw1G011450 Rw5G034150 Rw5G034170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 671
AccI GTMKAC 1 cut(s) 147
AclWI GGATC 3 cut(s) 216, 229, 841
AcsI RAATTY 2 cut(s) 57, 157
AfaI GTAC 2 cut(s) 546, 770
AfiI CCNNNNNNNGG 2 cut(s) 308, 671
AgsI TTSAA 4 cut(s) 229, 322, 551, 757
AluBI AGCT 5 cut(s) 137, 296, 509, 656, 774
AluI AGCT 5 cut(s) 137, 296, 509, 656, 774
AlwI GGATC 3 cut(s) 216, 229, 841
AlwNI CAGNNNCTG 1 cut(s) 126
Ama87I CYCGRG 1 cut(s) 379
AoxI GGCC 1 cut(s) 405
ApeKI GCWGC 1 cut(s) 653
ApoI RAATTY 2 cut(s) 57, 157
AspA2I CCTAGG 1 cut(s) 173
AspLEI GCGC 1 cut(s) 820
AsuHPI GGTGA 1 cut(s) 481
AvaI CYCGRG 1 cut(s) 379
AvrII CCTAGG 1 cut(s) 173
BamHI GGATCC 1 cut(s) 221
BbvI GCAGC 1 cut(s) 665
BccI CCATC 1 cut(s) 70
BcgI CGANNNNNNTGC 2 cut(s) 825, 859
BclI TGATCA 1 cut(s) 577
BfaI CTAG 2 cut(s) 174, 821
BisI GCNGC 1 cut(s) 654
BlnI CCTAGG 1 cut(s) 173
BlsI GCNGC 1 cut(s) 655
BmcAI AGTACT 1 cut(s) 546
BmeT110I CYCGRG 1 cut(s) 379
BmiI GGNNCC 2 cut(s) 142, 223
BmsI GCATC 3 cut(s) 30, 132, 695
BpmI CTGGAG 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 97
Bsa29I ATCGAT 1 cut(s) 619
BsaJI CCNNGG 3 cut(s) 173, 605, 630
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 2 cut(s) 308, 671
Bse1I ACTGG 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 601
BseCI ATCGAT 1 cut(s) 619
BseDI CCNNGG 3 cut(s) 173, 605, 630
BseGI GGATG 2 cut(s) 62, 710
BseLI CCNNNNNNNGG 2 cut(s) 308, 671
BseMI GCAATG 1 cut(s) 601
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 288
BseXI GCAGC 1 cut(s) 665
BshFI GGCC 1 cut(s) 407
BshVI ATCGAT 1 cut(s) 619
BsiHKCI CYCGRG 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 308, 671
BsmI GAATGC 2 cut(s) 172, 708
BsnI GGCC 1 cut(s) 407
BsoBI CYCGRG 1 cut(s) 379
Bsp143I GATC 6 cut(s) 221, 240, 577, 620, 846, 855
BspANI GGCC 1 cut(s) 407
BspDI ATCGAT 1 cut(s) 619
BspLI GGNNCC 2 cut(s) 142, 223
BspPI GGATC 3 cut(s) 216, 229, 841
BsrDI GCAATG 1 cut(s) 601
BsrI ACTGG 1 cut(s) 302
BssECI CCNNGG 3 cut(s) 173, 605, 630
BssMI GATC 6 cut(s) 221, 240, 577, 620, 846, 855
BssNAI GTATAC 1 cut(s) 148
BssT1I CCWWGG 2 cut(s) 173, 605
Bst1107I GTATAC 1 cut(s) 148
Bst4CI ACNGT 5 cut(s) 418, 649, 736, 828, 881
BstDSI CCRYGG 1 cut(s) 630
BstF5I GGATG 2 cut(s) 62, 710
BstHHI GCGC 1 cut(s) 820
BstKTI GATC 6 cut(s) 224, 243, 580, 623, 849, 858
BstMBI GATC 6 cut(s) 221, 240, 577, 620, 846, 855
BstMWI GCNNNNNNNGC 2 cut(s) 293, 454
BstV1I GCAGC 1 cut(s) 665
BstX2I RGATCY 1 cut(s) 221
BstYI RGATCY 1 cut(s) 221
BstZ17I GTATAC 1 cut(s) 148
Bsu15I ATCGAT 1 cut(s) 619
BsuRI GGCC 1 cut(s) 407
BsuTUI ATCGAT 1 cut(s) 619
BtgI CCRYGG 1 cut(s) 630
BtgZI GCGATG 1 cut(s) 229
BtsCI GGATG 2 cut(s) 62, 710
BtsIMutI CAGTG 1 cut(s) 373
CaiI CAGNNNCTG 1 cut(s) 126
CfoI GCGC 1 cut(s) 820
ClaI ATCGAT 1 cut(s) 619
Csp6I GTAC 2 cut(s) 545, 769
CspCI CAANNNNNGTGG 2 cut(s) 631, 666
CviJI RGCY 9 cut(s) 137, 296, 407, 448, 509, 641, 656, 760, 774
CviKI_1 RGCY 9 cut(s) 137, 296, 407, 448, 509, 641, 656, 760, 774
CviQI GTAC 2 cut(s) 545, 769
DpnI GATC 6 cut(s) 223, 242, 579, 622, 848, 857
DpnII GATC 6 cut(s) 221, 240, 577, 620, 846, 855
DraI TTTAAA 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 173, 605
Eco88I CYCGRG 1 cut(s) 379
EcoT14I CCWWGG 2 cut(s) 173, 605
EcoT22I ATGCAT 1 cut(s) 710
ErhI CCWWGG 2 cut(s) 173, 605
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FbaI TGATCA 1 cut(s) 577
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 1 cut(s) 654
FokI GGATG 2 cut(s) 49, 717
Fsp4HI GCNGC 1 cut(s) 654
FspBI CTAG 2 cut(s) 174, 821
GlaI GCGC 1 cut(s) 819
GluI GCNGC 1 cut(s) 654
GsuI CTGGAG 1 cut(s) 285
HaeIII GGCC 1 cut(s) 407
HhaI GCGC 1 cut(s) 820
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 2 cut(s) 208, 866
HphI GGTGA 1 cut(s) 481
Hpy166II GTNNAC 4 cut(s) 148, 203, 439, 565
Hpy188I TCNGA 2 cut(s) 398, 851
Hpy188III TCNNGA 5 cut(s) 37, 275, 472, 716, 863
Hpy8I GTNNAC 4 cut(s) 148, 203, 439, 565
HpyAV CCTTC 5 cut(s) 76, 172, 235, 404, 502
HpyCH4III ACNGT 5 cut(s) 418, 649, 736, 828, 881
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 6 cut(s) 4, 116, 499, 653, 708, 836
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 454
HpySE526I ACGT 1 cut(s) 150
HspAI GCGC 1 cut(s) 818
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 6 cut(s) 221, 240, 577, 620, 846, 855
LmnI GCTCC 3 cut(s) 175, 301, 695
LpnPI CCDG 8 cut(s) 29, 123, 269, 288, 315, 389, 701, 769
Lsp1109I GCAGC 1 cut(s) 665
LweI GCATC 3 cut(s) 30, 132, 695
MaeI CTAG 2 cut(s) 174, 821
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 532
MalI GATC 6 cut(s) 223, 242, 579, 622, 848, 857
MboI GATC 6 cut(s) 221, 240, 577, 620, 846, 855
MboII GAAGA 1 cut(s) 834
MflI RGATCY 1 cut(s) 221
MluCI AATT 8 cut(s) 14, 21, 57, 157, 351, 427, 523, 581
MmeI TCCRAC 2 cut(s) 133, 874
MnlI CCTC 5 cut(s) 72, 262, 309, 723, 772
Mph1103I ATGCAT 1 cut(s) 710
MseI TTAA 4 cut(s) 191, 306, 522, 806
MslI CAYNNNNRTG 1 cut(s) 703
MspA1I CMGCKG 2 cut(s) 137, 509
Mva1269I GAATGC 2 cut(s) 172, 708
MwoI GCNNNNNNNGC 2 cut(s) 293, 454
NdeII GATC 6 cut(s) 221, 240, 577, 620, 846, 855
NlaIV GGNNCC 2 cut(s) 142, 223
NsiI ATGCAT 1 cut(s) 710
PctI GAATGC 2 cut(s) 172, 708
PfeI GAWTC 2 cut(s) 208, 866
PflMI CCANNNNNTGG 1 cut(s) 671
PkrI GCNGC 1 cut(s) 655
PspN4I GGNNCC 2 cut(s) 142, 223
PstNI CAGNNNCTG 1 cut(s) 126
PsuI RGATCY 1 cut(s) 221
PvuII CAGCTG 2 cut(s) 137, 509
RsaI GTAC 2 cut(s) 546, 770
RsaNI GTAC 2 cut(s) 545, 769
RseI CAYNNNNRTG 1 cut(s) 703
SaqAI TTAA 4 cut(s) 191, 306, 522, 806
SatI GCNGC 1 cut(s) 654
Sau3AI GATC 6 cut(s) 221, 240, 577, 620, 846, 855
ScaI AGTACT 1 cut(s) 546
SfaNI GCATC 3 cut(s) 30, 132, 695
SmiMI CAYNNNNRTG 1 cut(s) 703
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
Sse9I AATT 8 cut(s) 14, 21, 57, 157, 351, 427, 523, 581
SspMI CTAG 2 cut(s) 174, 821
StyI CCWWGG 2 cut(s) 173, 605
TaaI ACNGT 5 cut(s) 418, 649, 736, 828, 881
TaiI ACGT 1 cut(s) 153
TaqI TCGA 4 cut(s) 239, 356, 619, 845
TasI AATT 8 cut(s) 14, 21, 57, 157, 351, 427, 523, 581
TatI WGTACW 2 cut(s) 544, 768
TfiI GAWTC 2 cut(s) 208, 866
Tru1I TTAA 4 cut(s) 191, 306, 522, 806
Tru9I TTAA 4 cut(s) 191, 306, 522, 806
TscAI CASTG 1 cut(s) 373
TseI GCWGC 1 cut(s) 653
TspDTI ATGAA 1 cut(s) 555
TspGWI ACGGA 3 cut(s) 133, 456, 619
TspRI CASTG 1 cut(s) 373
Van91I CCANNNNNTGG 1 cut(s) 671
XapI RAATTY 2 cut(s) 57, 157
XmaJI CCTAGG 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 2 cut(s) 174, 821
ZrmI AGTACT 1 cut(s) 546
Zsp2I ATGCAT 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.