Rw5G034150

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000597
Physical Location & Seq
Forward (+)
58663238 .. 58669747
6510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034150.1

Sequence Viewer

Length: 1416 bp
ATGGGGACTCTGGCACCAAACTCCATAGCAGCAGAAGCACACTATCGTAATCAAGATCAAGTAGTAGATGGTAATCATGCCAAGGTTGGTCAAGATGATGATGACCAACAGAACCAACTGGATGCCGGTGCCAAGTTTGTTCTTAAATCCAAAGGATCATGGGTGCACTGTGGTTATCATTTGACTACTTCAATAGTTGCTCCACCTCTGCTAAGTCTACCGTACGCTTTCACATTCCTCGGATGGACGGGCGGAGTTATTTGTCTGGTGATCGGAGCATTAGTAACTTTCTACTCGTACAACTTAATCTCTCTGGTGCTCGAGCATTATGCTCAATTGGGTCATCGCCAACTTCGCTTCAGAGACATGGCTCGTGACATTTTAGGACCCAAATGGGGTCGCTATTTCGTTGGTCCAATTCAGTTTATGGTATGCTATGGTGCTGTTGTGGCTTGTACTCTTTTGGGAGGGCAATGCATGAAGGCAGTTTACATGCTGACAAACCCAAATGGGAGCATGAAGCTGTATGAGTTTGTGATCATATTTGGATGCTTCATGCTGATTTTGGCCCAAATCCCATCTTTTCACTCACTACGGCACATCAACCTTGTCTCCATGATCCTTTGCCTAGCCTATAGTGTTTGTGCTACTGCTGCTTGCATCTATATTGGAAATTCTTCAAAAGGACCACCCAAGGACTATTCTTTGAAGGGCAACACCGAGAATCAGATTTTTGGGATATTTAATGCCAATGCCATCATTGCTACGACATACGGCAATGGTATCATTCCAGAAATTCAGGCAACAATCGCAGCACCAGTGAAGGGAAAGATGTTCAAGGGATTGTGTGTTTGTTACACAATAGTCACAATGACTTTCTTCAGCGTTGCGATATCTGGCTATTGGGCATTTGGTAACCAATCTGAAGGCCTCATCCTTAGCAACTTCTTGTCCGATGGCAACCCTTTGGTGCCGAAGTGGTTCATTTTCATGACGAACATTTTCACCATATTCCAGCTTTCCGCTGTTGGTGTGGTTTATCTGCAGCCCACAAATGAAGTACTAGAGAGAGCATTTGCAGACCCAACAAGGAAAGAATTTTCAGCTCGAAATGTGATCCCAAGGGTGATCTCTCGGTCACTATCTGTCATCACAGCAACAATCGTAGCAGCAATGCTTCCATTTTTTGGGGACATCAATGCAGTTATTGGGGCTTTTGGTTTCATGCCCCTCGACTTCATCTTGCCTGTTGTCTTCTTCAACTTGACCTTTAAGCCATCAAAACGAAGCCTTGTTTTCTGGTTGAACACCACCATTGCTGTGGTTTTTTCAGTCATGGGGGTTTTAGCAGCGATTGCAGCTGTAAGACAAATTAGCCTGGATGCCAGTTCTTACAAGTTATTTGCGAATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

471

Amino Acids

51.62

Weight (kDa)

8.86

Isoelectric Point (pI)

28.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 51 - 457 8.3e-65 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000597)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08230 AT1G08230 AT1G08230 AT1G08230 AT1G08230
fragaria_vesca FvH4_3g30140 FvH4_7g06340 FvH4_7g06350 FvH4_7g06350
malus_domestica MD02G1244800.v1.1 MD03G1133300.v1.1 MD07G1072100.v1.1 MD07G1072300.v1.1 MD11G1155800.v1.1 MD11G1156000.v1.1
prunus_persica Prupe.2G090300_v2.0.a1 Prupe.2G090500_v2.0.a1 Prupe.6G118000_v2.0.a1 Prupe.6G118000_v2.0.a1 Prupe.6G118000_v2.0.a1
pyrus_communis pycom02g21070 pycom03g08970 pycom07g05520
rosa_chinensis RchiOBHm_Chr1g0334311 RchiOBHm_Chr1g0334341 RchiOBHm_Chr1g0334351 RchiOBHm_Chr1g0334371 RchiOBHm_Chr1g0334381 RchiOBHm_Chr1g0334431 RchiOBHm_Chr5g0055491 RchiOBHm_Chr5g0055511
rosa_laevigata RLG00000029454 RLG00000029457 RLG00000029458 RLG00000029459 RLG00000029461 RLG00000034991 RLG00000034993
rosa_multiflora Rmu_sc0001673.1_g000036 Rmu_sc0001673.1_g000040 Rmu_sc0016880.1_g000002 Rmu_ssc0000047.1_g000014 Rmu_ssc0000047.1_g000015 Rmu_ssc0000047.1_g000016 Rmu_ssc0000047.1_g000023
rosa_roxburghii Rroxscaffold_1G00024820 Rroxscaffold_1G00024850 Rroxscaffold_1G00024860 Rroxscaffold_4G00316880 Rroxscaffold_4G00316900
rosa_rugosa Rorug01G0113300.1 Rorug01G0113400 Rorug01G0113500 Rorug05G0293200 Rorug05G0293200 Rorug05G0293400.1
rosa_samantha Rh1AG138200 Rh1AG138300 Rh1AG139000 Rh1BG104900 Rh1CG130400 Rh1CG130500 Rh1CG131000 Rh1DG142700 Rh1DG142800 Rh5AG362800 Rh5AG363000 Rh5BG375000 Rh5BG375200 Rh5CG396900 Rh5CG397100 Rh5DG388300 Rh5DG388400
rosa_wichuraiana Rw0G003720 Rw0G003730 Rw0G003740 Rw1G011440 Rw1G011450 Rw5G034150 Rw5G034170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 13, 128, 970
AccB7I CCANNNNNTGG 1 cut(s) 1187
AccI GTMKAC 1 cut(s) 217
AciI CCGC 2 cut(s) 252, 1023
AclWI GGATC 3 cut(s) 163, 613, 1111
AcsI RAATTY 4 cut(s) 673, 795, 1097, 1408
AcuI CTGAAG 3 cut(s) 343, 865, 945
AfaI GTAC 4 cut(s) 224, 299, 457, 1062
AfiI CCNNNNNNNGG 3 cut(s) 395, 824, 1187
AgsI TTSAA 6 cut(s) 192, 681, 709, 838, 1261, 1306
AjnI CCWGG 1 cut(s) 1377
AluBI AGCT 4 cut(s) 523, 1018, 1106, 1361
AluI AGCT 4 cut(s) 523, 1018, 1106, 1361
Alw21I GWGCWC 2 cut(s) 168, 321
Alw26I GTCTC 2 cut(s) 357, 616
Alw44I GTGCAC 1 cut(s) 164
AlwI GGATC 3 cut(s) 163, 613, 1111
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 2 cut(s) 567, 928
ApaLI GTGCAC 1 cut(s) 164
ApeKI GCWGC 7 cut(s) 29, 653, 812, 1045, 1169, 1349, 1358
ApoI RAATTY 4 cut(s) 673, 795, 1097, 1408
Asp700I GAANNNNTTC 3 cut(s) 676, 980, 1001
AspS9I GGNCC 4 cut(s) 386, 413, 568, 686
AsuHPI GGTGA 3 cut(s) 280, 997, 1138
AvaI CYCGRG 1 cut(s) 320
AvaII GGWCC 3 cut(s) 386, 413, 686
BaeGI GKGCMC 1 cut(s) 168
BanI GGYRCC 3 cut(s) 13, 128, 970
BarI GAAGNNNNNNTAC 2 cut(s) 473, 505
BauI CACGAG 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 1246
Bbv12I GWGCWC 2 cut(s) 168, 321
BbvI GCAGC 7 cut(s) 41, 640, 824, 1057, 1181, 1361, 1370
BccI CCATC 6 cut(s) 62, 237, 586, 764, 950, 1285
BceAI ACGGC 2 cut(s) 611, 790
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 1 cut(s) 1379
BclI TGATCA 1 cut(s) 537
BcoDI GTCTC 2 cut(s) 357, 616
BfaI CTAG 2 cut(s) 629, 1064
BfmI CTRYAG 2 cut(s) 634, 1043
BisI GCNGC 7 cut(s) 30, 654, 813, 1046, 1170, 1350, 1359
BlsI GCNGC 7 cut(s) 31, 655, 814, 1047, 1171, 1351, 1360
BmcAI AGTACT 1 cut(s) 1062
Bme1390I CCNGG 1 cut(s) 1379
Bme18I GGWCC 3 cut(s) 386, 413, 686
BmeT110I CYCGRG 1 cut(s) 320
BmgT120I GGNCC 4 cut(s) 386, 413, 568, 686
BmiI GGNNCC 4 cut(s) 15, 130, 388, 972
BmrFI CCNGG 1 cut(s) 1379
BmsI GCATC 4 cut(s) 112, 539, 669, 1372
BpiI GAAGAC 1 cut(s) 1246
Bpu10I CCTNAGC 1 cut(s) 938
BsaBI GATNNNNATC 1 cut(s) 72
BsaJI CCNNGG 4 cut(s) 81, 238, 693, 1121
BsaXI ACNNNNNCTCC 4 cut(s) 267, 297, 596, 626
Bsc4I CCNNNNNNNGG 3 cut(s) 395, 824, 1187
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 3 cut(s) 123, 818, 1386
Bse3DI GCAATG 5 cut(s) 479, 759, 784, 1179, 1314
Bse8I GATNNNNATC 1 cut(s) 72
BseBI CCWGG 1 cut(s) 1379
BseDI CCNNGG 4 cut(s) 81, 238, 693, 1121
BseGI GGATG 5 cut(s) 127, 248, 554, 933, 1387
BseJI GATNNNNATC 1 cut(s) 72
BseLI CCNNNNNNNGG 3 cut(s) 395, 824, 1187
BseMI GCAATG 5 cut(s) 479, 759, 784, 1179, 1314
BseNI ACTGG 3 cut(s) 123, 818, 1386
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 7 cut(s) 41, 640, 824, 1057, 1181, 1361, 1370
BshFI GGCC 2 cut(s) 569, 930
BshNI GGYRCC 3 cut(s) 13, 128, 970
BsiHKAI GWGCWC 2 cut(s) 168, 321
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 1 cut(s) 126
BsiWI CGTACG 1 cut(s) 222
BslFI GGGAC 2 cut(s) 19, 1205
BslI CCNNNNNNNGG 3 cut(s) 395, 824, 1187
BsmAI GTCTC 2 cut(s) 357, 616
BsmFI GGGAC 2 cut(s) 19, 1205
BsnI GGCC 2 cut(s) 569, 930
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 2 cut(s) 168, 321
Bsp143I GATC 7 cut(s) 55, 155, 270, 537, 618, 1116, 1128
BspACI CCGC 2 cut(s) 252, 1023
BspANI GGCC 2 cut(s) 569, 930
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 4 cut(s) 15, 130, 388, 972
BspMAI CTGCAG 1 cut(s) 1047
BspPI GGATC 3 cut(s) 163, 613, 1111
BspT107I GGYRCC 3 cut(s) 13, 128, 970
BsrDI GCAATG 5 cut(s) 479, 759, 784, 1179, 1314
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 3 cut(s) 123, 818, 1386
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 4 cut(s) 81, 238, 693, 1121
BssMI GATC 7 cut(s) 55, 155, 270, 537, 618, 1116, 1128
BssSI CACGAG 1 cut(s) 372
BssT1I CCWWGG 3 cut(s) 81, 693, 1121
Bst2BI CACGAG 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 1379
Bst4CI ACNGT 2 cut(s) 170, 222
BstAPI GCANNNNNTGC 1 cut(s) 1355
BstC8I GCNNGC 1 cut(s) 658
BstDEI CTNAG 2 cut(s) 212, 938
BstEII GGTNACC 1 cut(s) 914
BstF5I GGATG 5 cut(s) 127, 248, 554, 933, 1387
BstKTI GATC 7 cut(s) 58, 158, 273, 540, 621, 1119, 1131
BstMAI GTCTC 2 cut(s) 357, 616
BstMBI GATC 7 cut(s) 55, 155, 270, 537, 618, 1116, 1128
BstMWI GCNNNNNNNGC 8 cut(s) 35, 354, 449, 653, 761, 809, 1355, 1358
BstNI CCWGG 1 cut(s) 1379
BstNSI RCATGY 1 cut(s) 496
BstPI GGTNACC 1 cut(s) 914
BstSCI CCNGG 1 cut(s) 1377
BstSFI CTRYAG 2 cut(s) 634, 1043
BstSLI GKGCMC 1 cut(s) 168
BstV1I GCAGC 7 cut(s) 41, 640, 824, 1057, 1181, 1361, 1370
BstV2I GAAGAC 1 cut(s) 1246
BstXI CCANNNNNNTGG 1 cut(s) 1321
BsuRI GGCC 2 cut(s) 569, 930
BtgZI GCGATG 1 cut(s) 329
BtsCI GGATG 5 cut(s) 127, 248, 554, 933, 1387
BtsIMutI CAGTG 2 cut(s) 166, 825
Cac8I GCNNGC 1 cut(s) 658
CciI TCATGA 1 cut(s) 990
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 4 cut(s) 386, 413, 568, 686
Csp6I GTAC 4 cut(s) 223, 298, 456, 1061
CviQI GTAC 4 cut(s) 223, 298, 456, 1061
DdeI CTNAG 2 cut(s) 212, 938
DpnI GATC 7 cut(s) 57, 157, 272, 539, 620, 1118, 1130
DpnII GATC 7 cut(s) 55, 155, 270, 537, 618, 1116, 1128
EciI GGCGGA 1 cut(s) 267
Eco130I CCWWGG 3 cut(s) 81, 693, 1121
Eco147I AGGCCT 1 cut(s) 930
Eco32I GATATC 1 cut(s) 894
Eco47I GGWCC 3 cut(s) 386, 413, 686
Eco57I CTGAAG 3 cut(s) 343, 865, 945
Eco88I CYCGRG 1 cut(s) 320
Eco91I GGTNACC 1 cut(s) 914
EcoO109I RGGNCCY 1 cut(s) 386
EcoO65I GGTNACC 1 cut(s) 914
EcoRII CCWGG 1 cut(s) 1377
EcoRV GATATC 1 cut(s) 894
EcoT14I CCWWGG 3 cut(s) 81, 693, 1121
EcoT22I ATGCAT 1 cut(s) 479
ErhI CCWWGG 3 cut(s) 81, 693, 1121
FaqI GGGAC 2 cut(s) 19, 1205
FbaI TGATCA 1 cut(s) 537
FblI GTMKAC 1 cut(s) 217
Fnu4HI GCNGC 7 cut(s) 30, 654, 813, 1046, 1170, 1350, 1359
FokI GGATG 5 cut(s) 134, 255, 561, 920, 1394
Fsp4HI GCNGC 7 cut(s) 30, 654, 813, 1046, 1170, 1350, 1359
FspBI CTAG 2 cut(s) 629, 1064
GluI GCNGC 7 cut(s) 30, 654, 813, 1046, 1170, 1350, 1359
HaeIII GGCC 2 cut(s) 569, 930
HapII CCGG 1 cut(s) 126
HinfI GANTC 2 cut(s) 7, 724
HpaII CCGG 1 cut(s) 126
HphI GGTGA 3 cut(s) 280, 997, 1138
Hpy166II GTNNAC 3 cut(s) 166, 218, 490
Hpy188I TCNGA 6 cut(s) 242, 275, 362, 729, 925, 955
Hpy188III TCNNGA 5 cut(s) 53, 92, 374, 791, 991
Hpy8I GTNNAC 3 cut(s) 166, 218, 490
HpyAV CCTTC 4 cut(s) 475, 703, 817, 920
HpyCH4III ACNGT 2 cut(s) 170, 222
HpyCH4V TGCA 7 cut(s) 166, 477, 660, 1045, 1079, 1202, 1358
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 354, 449, 653, 761, 809, 1355, 1358
HpyF3I CTNAG 2 cut(s) 212, 938
Ksp22I TGATCA 1 cut(s) 537
Kzo9I GATC 7 cut(s) 55, 155, 270, 537, 618, 1116, 1128
LmnI GCTCC 3 cut(s) 205, 275, 513
Lsp1109I GCAGC 7 cut(s) 41, 640, 824, 1057, 1181, 1361, 1370
LweI GCATC 4 cut(s) 112, 539, 669, 1372
MaeI CTAG 2 cut(s) 629, 1064
MaeIII GTNAC 6 cut(s) 283, 374, 854, 865, 914, 1137
MalI GATC 7 cut(s) 57, 157, 272, 539, 620, 1118, 1130
MboI GATC 7 cut(s) 55, 155, 270, 537, 618, 1116, 1128
MboII GAAGA 4 cut(s) 669, 871, 1246, 1249
MfeI CAATTG 1 cut(s) 335
MhlI GDGCHC 2 cut(s) 168, 321
MluCI AATT 7 cut(s) 335, 417, 673, 795, 1097, 1371, 1408
MnlI CCTC 5 cut(s) 216, 248, 461, 941, 1241
Mph1103I ATGCAT 1 cut(s) 479
MroXI GAANNNNTTC 3 cut(s) 676, 980, 1001
MseI TTAA 4 cut(s) 144, 305, 744, 1272
MslI CAYNNNNRTG 2 cut(s) 989, 1319
MspA1I CMGCKG 2 cut(s) 1025, 1361
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 1 cut(s) 1379
MunI CAATTG 1 cut(s) 335
MvaI CCWGG 1 cut(s) 1379
MwoI GCNNNNNNNGC 8 cut(s) 35, 354, 449, 653, 761, 809, 1355, 1358
NdeII GATC 7 cut(s) 55, 155, 270, 537, 618, 1116, 1128
NlaIV GGNNCC 4 cut(s) 15, 130, 388, 972
NmuCI GTSAC 3 cut(s) 374, 865, 1137
NsiI ATGCAT 1 cut(s) 479
NspI RCATGY 1 cut(s) 496
PaeR7I CTCGAG 1 cut(s) 320
PagI TCATGA 1 cut(s) 990
PceI AGGCCT 1 cut(s) 930
PdmI GAANNNNTTC 3 cut(s) 676, 980, 1001
PfeI GAWTC 1 cut(s) 724
Pfl23II CGTACG 1 cut(s) 222
PflMI CCANNNNNTGG 1 cut(s) 1187
PkrI GCNGC 7 cut(s) 31, 655, 814, 1047, 1171, 1351, 1360
PpuMI RGGWCCY 1 cut(s) 386
Psp5II RGGWCCY 1 cut(s) 386
Psp6I CCWGG 1 cut(s) 1377
PspEI GGTNACC 1 cut(s) 914
PspGI CCWGG 1 cut(s) 1377
PspLI CGTACG 1 cut(s) 222
PspN4I GGNNCC 4 cut(s) 15, 130, 388, 972
PspPI GGNCC 4 cut(s) 386, 413, 568, 686
PspPPI RGGWCCY 1 cut(s) 386
PspXI VCTCGAGB 1 cut(s) 320
PstI CTGCAG 1 cut(s) 1047
PvuII CAGCTG 1 cut(s) 1361
RsaI GTAC 4 cut(s) 224, 299, 457, 1062
RsaNI GTAC 4 cut(s) 223, 298, 456, 1061
RseI CAYNNNNRTG 2 cut(s) 989, 1319
SaqAI TTAA 4 cut(s) 144, 305, 744, 1272
SatI GCNGC 7 cut(s) 30, 654, 813, 1046, 1170, 1350, 1359
Sau3AI GATC 7 cut(s) 55, 155, 270, 537, 618, 1116, 1128
Sau96I GGNCC 4 cut(s) 386, 413, 568, 686
ScaI AGTACT 1 cut(s) 1062
ScrFI CCNGG 1 cut(s) 1379
SduI GDGCHC 2 cut(s) 168, 321
SetI ASST 8 cut(s) 87, 208, 525, 609, 1020, 1108, 1271, 1363
SfaNI GCATC 4 cut(s) 112, 539, 669, 1372
SfcI CTRYAG 2 cut(s) 634, 1043
Sfr274I CTCGAG 1 cut(s) 320
SinI GGWCC 3 cut(s) 386, 413, 686
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 2 cut(s) 989, 1319
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 7 cut(s) 335, 417, 673, 795, 1097, 1371, 1408
SseBI AGGCCT 1 cut(s) 930
SsiI CCGC 2 cut(s) 252, 1023
SspMI CTAG 2 cut(s) 629, 1064
StuI AGGCCT 1 cut(s) 930
StyD4I CCNGG 1 cut(s) 1377
StyI CCWWGG 3 cut(s) 81, 693, 1121
TaaI ACNGT 2 cut(s) 170, 222
TaqI TCGA 3 cut(s) 321, 1108, 1233
TaqII GACCGA 1 cut(s) 1125
TasI AATT 7 cut(s) 335, 417, 673, 795, 1097, 1371, 1408
TatI WGTACW 2 cut(s) 455, 1060
TfiI GAWTC 1 cut(s) 724
Tru1I TTAA 4 cut(s) 144, 305, 744, 1272
Tru9I TTAA 4 cut(s) 144, 305, 744, 1272
TscAI CASTG 2 cut(s) 173, 825
TseFI GTSAC 3 cut(s) 374, 865, 1137
TseI GCWGC 7 cut(s) 29, 653, 812, 1045, 1169, 1349, 1358
Tsp45I GTSAC 3 cut(s) 374, 865, 1137
TspDTI ATGAA 8 cut(s) 494, 533, 544, 973, 979, 1071, 1213, 1228
TspRI CASTG 2 cut(s) 173, 825
Van91I CCANNNNNTGG 1 cut(s) 1187
VneI GTGCAC 1 cut(s) 164
VpaK11BI GGWCC 3 cut(s) 386, 413, 686
XapI RAATTY 4 cut(s) 673, 795, 1097, 1408
XceI RCATGY 1 cut(s) 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 1318
XhoI CTCGAG 1 cut(s) 320
XmiI GTMKAC 1 cut(s) 217
XmnI GAANNNNTTC 3 cut(s) 676, 980, 1001
XspI CTAG 2 cut(s) 629, 1064
ZrmI AGTACT 1 cut(s) 1062
Zsp2I ATGCAT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.