pycom07g05520

GABA transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000597
Physical Location & Seq
Forward (+)
4794540 .. 4797304
2765 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g05520.4

Sequence Viewer

Length: 1383 bp
ATGGAGTCAGTTCTTCCCAGTTCAAATACTGATTTATCAGCAGAAAATAAGGAAGATGAAGAAGTCAACATGTCAAAGGAGCTTGATGCTGGAGCTTTATTTGTGCTCAAATCTAGAGGGTCATGGTTGCATTGTGGATATCACCTGACAACTTCAATTGTAGCTCCTGCCCTTTTAAGTCTTCCCTATGCACTTTCCTTGATGGGTTGGTTTGCTGGGGCTTTATGCCTCACCGTTTCAGCTCTTGTAACGTTCTACTCCTACAACCTTCTGTCCTTGGTTTTAGAACACCATGACCAGCTTGGCATGCGCCAGCTTCGATTCCGCGACATGGCCAGAGATATTTTGGGACCATGGTGGGGAAGATACTTTGTTGGTCCAATTCAATTTGGCTTGTGCTATGGTGCAGTTATAGCTTGCATTCTTCTGGGAGGGCAGAGCCTTAAGTACATATATTTGCTTTCTAGTTCCAGACCAGAAGCCATGCAACTCTACCAATTTGTTATTATTTTTGGTGTCTTGATGCTGGTGTTGGCACAAATTCCATCCTTCCACTCCTTGAGGCATATCAACCTTGTCTCTCTAATCCTTTGTCTTGCATATAGCATCTGTGCCACAGCTGGTTCCGTATATATCGGAAATTCGACTAATGCTCCTAGGAAGGACTACTCTTTAAGCGGAAGTGGACAGAATCGTGTTTTTGGATCCTTCAGCGCTATTTCAATCATCGCAACCACATATGGAAATGGCATTATTCCTGAAATACAGGCAACAATAGCACCTCCAGTTAAAGGAAAAATGTTCAAAGGATTATGCGTATGCTATGCTGTTGTACTTTCGACATTTTTCAGTGTTGGTATCTCGGGTTATTGGGCGTTTGGAAATCAGGCCAAGGGAACAATTCTGCTCAATTTTTTAGTTGACGGAAAGCCTCTGTTGCCAACTTGGATTCTATTGATGACCAATGTTTTCACCTTCTTGCAAGTATCAGCTGTTAGTGTGGTTTACTTACAACCAACAAATGAAGTACTTGAACGCAAGTTTGCGAATGCAAAGGTTGATCAGTTCTCTGCTCGCAATGTTGTGCCAAGGTTGGTTTATCGATCTCTGTCTGTGGTCATAGCCACAACTGTTGCAGCTATGTTTCCATTCTTTGGTGACATCAACGCTCTGATTGGAGCATTTGGATGCATTCCTCTGGACTTCATTTTGCCAATGGTGTTCTACAATGTTGTATTCAAGCCCTCCAAGTACAGCTTACTTTTCTGGGGAAACACAATCATTGCCTCAACATTTTCTGCACTAGGACTGTTGGGTGCAATATCTTCGATCCGACAGATCGTTCTTGATTCTAACACCTACAGTTTTTTTGCTAACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

461

Amino Acids

50.4

Weight (kDa)

8.75

Isoelectric Point (pI)

27.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000597)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08230 AT1G08230 AT1G08230 AT1G08230 AT1G08230
fragaria_vesca FvH4_3g30140 FvH4_7g06340 FvH4_7g06350 FvH4_7g06350
malus_domestica MD02G1244800.v1.1 MD03G1133300.v1.1 MD07G1072100.v1.1 MD07G1072300.v1.1 MD11G1155800.v1.1 MD11G1156000.v1.1
prunus_persica Prupe.2G090300_v2.0.a1 Prupe.2G090500_v2.0.a1 Prupe.6G118000_v2.0.a1 Prupe.6G118000_v2.0.a1 Prupe.6G118000_v2.0.a1
pyrus_communis pycom02g21070 pycom03g08970 pycom07g05520
rosa_chinensis RchiOBHm_Chr1g0334311 RchiOBHm_Chr1g0334341 RchiOBHm_Chr1g0334351 RchiOBHm_Chr1g0334371 RchiOBHm_Chr1g0334381 RchiOBHm_Chr1g0334431 RchiOBHm_Chr5g0055491 RchiOBHm_Chr5g0055511
rosa_laevigata RLG00000029454 RLG00000029457 RLG00000029458 RLG00000029459 RLG00000029461 RLG00000034991 RLG00000034993
rosa_multiflora Rmu_sc0001673.1_g000036 Rmu_sc0001673.1_g000040 Rmu_sc0016880.1_g000002 Rmu_ssc0000047.1_g000014 Rmu_ssc0000047.1_g000015 Rmu_ssc0000047.1_g000016 Rmu_ssc0000047.1_g000023
rosa_roxburghii Rroxscaffold_1G00024820 Rroxscaffold_1G00024850 Rroxscaffold_1G00024860 Rroxscaffold_4G00316880 Rroxscaffold_4G00316900
rosa_rugosa Rorug01G0113300.1 Rorug01G0113400 Rorug01G0113500 Rorug05G0293200 Rorug05G0293200 Rorug05G0293400.1
rosa_samantha Rh1AG138200 Rh1AG138300 Rh1AG139000 Rh1BG104900 Rh1CG130400 Rh1CG130500 Rh1CG131000 Rh1DG142700 Rh1DG142800 Rh5AG362800 Rh5AG363000 Rh5BG375000 Rh5BG375200 Rh5CG396900 Rh5CG397100 Rh5DG388300 Rh5DG388400
rosa_wichuraiana Rw0G003720 Rw0G003730 Rw0G003740 Rw1G011440 Rw1G011450 Rw5G034150 Rw5G034170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1154
AccII CGCG 1 cut(s) 327
AciI CCGC 2 cut(s) 325, 678
AclI AACGTT 1 cut(s) 251
AclWI GGATC 3 cut(s) 699, 712, 1324
AcoI YGGCCR 1 cut(s) 333
AcsI RAATTY 2 cut(s) 540, 640
AcuI CTGAAG 1 cut(s) 694
AfaI GTAC 4 cut(s) 449, 834, 1029, 1253
AfeI AGCGCT 1 cut(s) 715
AfiI CCNNNNNNNGG 4 cut(s) 331, 359, 791, 1154
AflII CTTAAG 1 cut(s) 443
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 7 cut(s) 24, 156, 386, 723, 805, 1034, 1240
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 1 cut(s) 583
AlwI GGATC 3 cut(s) 699, 712, 1324
Ama87I CYCGRG 1 cut(s) 862
Aor51HI AGCGCT 1 cut(s) 715
AoxI GGCC 2 cut(s) 333, 888
ApeKI GCWGC 1 cut(s) 1136
ApoI RAATTY 2 cut(s) 540, 640
AspA2I CCTAGG 1 cut(s) 656
AspLEI GCGC 2 cut(s) 312, 716
AspS9I GGNCC 2 cut(s) 350, 377
AsuHPI GGTGA 4 cut(s) 134, 223, 964, 1169
AvaI CYCGRG 1 cut(s) 862
AvaII GGWCC 2 cut(s) 350, 377
AvrII CCTAGG 1 cut(s) 656
BalI TGGCCA 1 cut(s) 335
BamHI GGATCC 1 cut(s) 704
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 1 cut(s) 1148
BccI CCATC 2 cut(s) 196, 553
BcgI CGANNNNNNTGC 2 cut(s) 1308, 1342
BclI TGATCA 1 cut(s) 1060
BcoDI GTCTC 1 cut(s) 583
BfaI CTAG 4 cut(s) 114, 465, 657, 1304
BfmI CTRYAG 1 cut(s) 1360
BfoI RGCGCY 1 cut(s) 717
BfrI CTTAAG 1 cut(s) 443
BisI GCNGC 1 cut(s) 1137
BlnI CCTAGG 1 cut(s) 656
BlsI GCNGC 1 cut(s) 1138
BmcAI AGTACT 1 cut(s) 1029
Bme18I GGWCC 2 cut(s) 350, 377
BmeT110I CYCGRG 1 cut(s) 862
BmgT120I GGNCC 2 cut(s) 350, 377
BmiI GGNNCC 3 cut(s) 351, 625, 706
BmrI ACTGGG 1 cut(s) 12
BmsI GCATC 4 cut(s) 76, 513, 615, 1178
BmuI ACTGGG 1 cut(s) 12
BpiI GAAGAC 1 cut(s) 173
BpmI CTGGAG 2 cut(s) 111, 768
BpuEI CTTGAG 1 cut(s) 580
Bsa29I ATCGAT 1 cut(s) 1102
BsaJI CCNNGG 5 cut(s) 276, 353, 656, 891, 1088
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 359, 791, 1154
Bse1I ACTGG 2 cut(s) 18, 785
Bse3DI GCAATG 2 cut(s) 1084, 1281
BseCI ATCGAT 1 cut(s) 1102
BseDI CCNNGG 5 cut(s) 276, 353, 656, 891, 1088
BseGI GGATG 2 cut(s) 545, 1193
BseLI CCNNNNNNNGG 4 cut(s) 331, 359, 791, 1154
BseMI GCAATG 2 cut(s) 1084, 1281
BseNI ACTGG 2 cut(s) 18, 785
BseXI GCAGC 1 cut(s) 1148
BseYI CCCAGC 1 cut(s) 215
BsgI GTGCAG 2 cut(s) 426, 1284
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 2 cut(s) 335, 890
BshVI ATCGAT 1 cut(s) 1102
BsiHKAI GWGCWC 1 cut(s) 108
BsiHKCI CYCGRG 1 cut(s) 862
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 4 cut(s) 331, 359, 791, 1154
BsmAI GTCTC 1 cut(s) 583
BsmFI GGGAC 1 cut(s) 363
BsmI GAATGC 3 cut(s) 420, 1054, 1191
BsnI GGCC 2 cut(s) 335, 890
BsoBI CYCGRG 1 cut(s) 862
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 5 cut(s) 704, 1060, 1103, 1329, 1338
Bsp19I CCATGG 1 cut(s) 353
BspACI CCGC 2 cut(s) 325, 678
BspANI GGCC 2 cut(s) 335, 890
BspDI ATCGAT 1 cut(s) 1102
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 3 cut(s) 351, 625, 706
BspPI GGATC 3 cut(s) 699, 712, 1324
BspTI CTTAAG 1 cut(s) 443
BsrDI GCAATG 2 cut(s) 1084, 1281
BsrI ACTGG 2 cut(s) 18, 785
BssECI CCNNGG 5 cut(s) 276, 353, 656, 891, 1088
BssMI GATC 5 cut(s) 704, 1060, 1103, 1329, 1338
BssT1I CCWWGG 5 cut(s) 276, 353, 656, 891, 1088
Bst4CI ACNGT 4 cut(s) 235, 1132, 1311, 1364
BstAFI CTTAAG 1 cut(s) 443
BstC8I GCNNGC 4 cut(s) 308, 314, 418, 1075
BstDSI CCRYGG 1 cut(s) 353
BstF5I GGATG 2 cut(s) 545, 1193
BstFNI CGCG 1 cut(s) 327
BstH2I RGCGCY 1 cut(s) 717
BstHHI GCGC 2 cut(s) 312, 716
BstKTI GATC 5 cut(s) 707, 1063, 1106, 1332, 1341
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 5 cut(s) 704, 1060, 1103, 1329, 1338
BstMWI GCNNNNNNNGC 4 cut(s) 307, 413, 776, 937
BstNSI RCATGY 2 cut(s) 73, 310
BstSFI CTRYAG 1 cut(s) 1360
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 1 cut(s) 1148
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 1 cut(s) 704
BstYI RGATCY 1 cut(s) 704
Bsu15I ATCGAT 1 cut(s) 1102
BsuRI GGCC 2 cut(s) 335, 890
BsuTUI ATCGAT 1 cut(s) 1102
BtgI CCRYGG 1 cut(s) 353
BtgZI GCGATG 1 cut(s) 712
BtsCI GGATG 2 cut(s) 545, 1193
BtsIMutI CAGTG 1 cut(s) 856
Cac8I GCNNGC 4 cut(s) 308, 314, 418, 1075
CfoI GCGC 2 cut(s) 312, 716
Cfr13I GGNCC 2 cut(s) 350, 377
ClaI ATCGAT 1 cut(s) 1102
Csp6I GTAC 4 cut(s) 448, 833, 1028, 1252
CspCI CAANNNNNGTGG 2 cut(s) 1114, 1149
CviAII CATG 7 cut(s) 70, 123, 293, 307, 331, 354, 484
CviQI GTAC 4 cut(s) 448, 833, 1028, 1252
DpnI GATC 5 cut(s) 706, 1062, 1105, 1331, 1340
DpnII GATC 5 cut(s) 704, 1060, 1103, 1329, 1338
EaeI YGGCCR 1 cut(s) 333
Eco130I CCWWGG 5 cut(s) 276, 353, 656, 891, 1088
Eco32I GATATC 1 cut(s) 140
Eco47I GGWCC 2 cut(s) 350, 377
Eco47III AGCGCT 1 cut(s) 715
Eco57I CTGAAG 1 cut(s) 694
Eco88I CYCGRG 1 cut(s) 862
EcoRV GATATC 1 cut(s) 140
EcoT14I CCWWGG 5 cut(s) 276, 353, 656, 891, 1088
EcoT22I ATGCAT 1 cut(s) 1193
ErhI CCWWGG 5 cut(s) 276, 353, 656, 891, 1088
FaeI CATG 7 cut(s) 73, 126, 296, 310, 334, 357, 487
FalI AAGNNNNNCTT 2 cut(s) 1241, 1273
FaqI GGGAC 1 cut(s) 363
FatI CATG 7 cut(s) 69, 122, 292, 306, 330, 353, 483
FauNDI CATATG 1 cut(s) 739
FbaI TGATCA 1 cut(s) 1060
Fnu4HI GCNGC 1 cut(s) 1137
FokI GGATG 2 cut(s) 532, 1200
Fsp4HI GCNGC 1 cut(s) 1137
FspBI CTAG 4 cut(s) 114, 465, 657, 1304
GlaI GCGC 2 cut(s) 311, 715
GluI GCNGC 1 cut(s) 1137
GsaI CCCAGC 1 cut(s) 219
GsuI CTGGAG 2 cut(s) 111, 768
HaeII RGCGCY 1 cut(s) 717
HaeIII GGCC 2 cut(s) 335, 890
HhaI GCGC 2 cut(s) 312, 716
Hin1II CATG 7 cut(s) 73, 126, 296, 310, 334, 357, 487
Hin6I GCGC 2 cut(s) 310, 714
HinP1I GCGC 2 cut(s) 310, 714
HincII GTYRAC 2 cut(s) 67, 922
HindII GTYRAC 2 cut(s) 67, 922
HinfI GANTC 5 cut(s) 5, 321, 691, 949, 1349
HphI GGTGA 4 cut(s) 134, 223, 964, 1169
Hpy166II GTNNAC 4 cut(s) 67, 686, 922, 1006
Hpy188I TCNGA 3 cut(s) 638, 1173, 1334
Hpy188III TCNNGA 6 cut(s) 114, 471, 520, 758, 1199, 1346
Hpy8I GTNNAC 4 cut(s) 67, 686, 922, 1006
HpyAV CCTTC 5 cut(s) 278, 559, 655, 718, 985
HpyCH4III ACNGT 4 cut(s) 235, 1132, 1311, 1364
HpyCH4IV ACGT 1 cut(s) 251
HpyF10VI GCNNNNNNNGC 4 cut(s) 307, 413, 776, 937
HpySE526I ACGT 1 cut(s) 251
Hsp92II CATG 7 cut(s) 73, 126, 296, 310, 334, 357, 487
HspAI GCGC 2 cut(s) 310, 714
Ksp22I TGATCA 1 cut(s) 1060
Kzo9I GATC 5 cut(s) 704, 1060, 1103, 1329, 1338
LmnI GCTCC 5 cut(s) 79, 92, 169, 658, 1178
Lsp1109I GCAGC 1 cut(s) 1148
LweI GCATC 4 cut(s) 76, 513, 615, 1178
MaeI CTAG 4 cut(s) 114, 465, 657, 1304
MaeII ACGT 1 cut(s) 251
MaeIII GTNAC 2 cut(s) 247, 1157
MalI GATC 5 cut(s) 706, 1062, 1105, 1331, 1340
MboI GATC 5 cut(s) 704, 1060, 1103, 1329, 1338
MboII GAAGA 7 cut(s) 5, 65, 71, 173, 375, 416, 1317
MfeI CAATTG 1 cut(s) 156
MflI RGATCY 1 cut(s) 704
MhlI GDGCHC 1 cut(s) 108
MlsI TGGCCA 1 cut(s) 335
MluCI AATT 8 cut(s) 156, 381, 386, 497, 540, 640, 900, 910
MluNI TGGCCA 1 cut(s) 335
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 1357
MnlI CCTC 9 cut(s) 110, 239, 425, 555, 792, 942, 1206, 1255, 1297
Mox20I TGGCCA 1 cut(s) 335
Mph1103I ATGCAT 1 cut(s) 1193
MscI TGGCCA 1 cut(s) 335
MseI TTAA 4 cut(s) 176, 444, 674, 789
MslI CAYNNNNRTG 1 cut(s) 1186
Msp20I TGGCCA 1 cut(s) 335
MspA1I CMGCKG 2 cut(s) 620, 992
MspCI CTTAAG 1 cut(s) 443
MunI CAATTG 1 cut(s) 156
Mva1269I GAATGC 3 cut(s) 420, 1054, 1191
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 4 cut(s) 307, 413, 776, 937
NcoI CCATGG 1 cut(s) 353
NdeI CATATG 1 cut(s) 739
NdeII GATC 5 cut(s) 704, 1060, 1103, 1329, 1338
NlaIII CATG 7 cut(s) 73, 126, 296, 310, 334, 357, 487
NlaIV GGNNCC 3 cut(s) 351, 625, 706
NmuCI GTSAC 1 cut(s) 1157
NsiI ATGCAT 1 cut(s) 1193
NspI RCATGY 2 cut(s) 73, 310
PaeI GCATGC 1 cut(s) 310
PciI ACATGT 1 cut(s) 69
PctI GAATGC 3 cut(s) 420, 1054, 1191
PfeI GAWTC 4 cut(s) 321, 691, 949, 1349
PflMI CCANNNNNTGG 1 cut(s) 1154
PkrI GCNGC 1 cut(s) 1138
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PscI ACATGT 1 cut(s) 69
Psp1406I AACGTT 1 cut(s) 251
PspFI CCCAGC 1 cut(s) 215
PspN4I GGNNCC 3 cut(s) 351, 625, 706
PspPI GGNCC 2 cut(s) 350, 377
PsuI RGATCY 1 cut(s) 704
PvuII CAGCTG 2 cut(s) 620, 992
RsaI GTAC 4 cut(s) 449, 834, 1029, 1253
RsaNI GTAC 4 cut(s) 448, 833, 1028, 1252
RseI CAYNNNNRTG 1 cut(s) 1186
SaqAI TTAA 4 cut(s) 176, 444, 674, 789
SatI GCNGC 1 cut(s) 1137
Sau3AI GATC 5 cut(s) 704, 1060, 1103, 1329, 1338
Sau96I GGNCC 2 cut(s) 350, 377
ScaI AGTACT 1 cut(s) 1029
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 1 cut(s) 108
SfaNI GCATC 4 cut(s) 76, 513, 615, 1178
SfcI CTRYAG 1 cut(s) 1360
SinI GGWCC 2 cut(s) 350, 377
SmiMI CAYNNNNRTG 1 cut(s) 1186
SmlI CTYRAG 2 cut(s) 443, 559
SmoI CTYRAG 2 cut(s) 443, 559
SphI GCATGC 1 cut(s) 310
Sse9I AATT 8 cut(s) 156, 381, 386, 497, 540, 640, 900, 910
SsiI CCGC 2 cut(s) 325, 678
SspMI CTAG 4 cut(s) 114, 465, 657, 1304
StyI CCWWGG 5 cut(s) 276, 353, 656, 891, 1088
TaaI ACNGT 4 cut(s) 235, 1132, 1311, 1364
TaiI ACGT 1 cut(s) 254
TaqI TCGA 5 cut(s) 319, 644, 839, 1102, 1328
TasI AATT 8 cut(s) 156, 381, 386, 497, 540, 640, 900, 910
TatI WGTACW 4 cut(s) 447, 832, 1027, 1251
TfiI GAWTC 4 cut(s) 321, 691, 949, 1349
Tru1I TTAA 4 cut(s) 176, 444, 674, 789
Tru9I TTAA 4 cut(s) 176, 444, 674, 789
TscAI CASTG 1 cut(s) 856
TseFI GTSAC 1 cut(s) 1157
TseI GCWGC 1 cut(s) 1136
Tsp45I GTSAC 1 cut(s) 1157
TspDTI ATGAA 3 cut(s) 72, 1038, 1195
TspGWI ACGGA 2 cut(s) 616, 939
TspRI CASTG 1 cut(s) 856
Van91I CCANNNNNTGG 1 cut(s) 1154
Vha464I CTTAAG 1 cut(s) 443
VpaK11BI GGWCC 2 cut(s) 350, 377
XapI RAATTY 2 cut(s) 540, 640
XbaI TCTAGA 1 cut(s) 113
XceI RCATGY 2 cut(s) 73, 310
XcmI CCANNNNNNNNNTGG 2 cut(s) 299, 343
XmaJI CCTAGG 1 cut(s) 656
XspI CTAG 4 cut(s) 114, 465, 657, 1304
ZrmI AGTACT 1 cut(s) 1029
Zsp2I ATGCAT 1 cut(s) 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.