MD10G1219500.v1.1

Belongs to the isocitrate and isopropylmalate dehydrogenases family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001254
Physical Location & Seq
Forward (+)
31704442 .. 31706912
2471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1219500.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGACAAGTCAAATTGTTTGTGTTAGGCACAATTTGTACAATGTAGCAATAAAGTGTGCAACTATAACTCCAGATGAAACTCGTGTGAAGGAGTTCAACTTGAAACAGATGTGGAGGAGTCCAAATGGGACCATACGGAACATTTTAAATGGTACTGTTTTTAGAGAACCTATTCTGCAGAAATGTTCCCCGTCTTGTTCCAGGTTTGCTACAAGTTGGACAATGCCAATATGCATTGGGAGACATGCTTTTGGTGTTCAGTACCAAGCAACTGATGCAATCATAGAAGGACCTGGAAAACTTAAATTGGTTTTTGTACCTGATGGGCCCTATGAGAAGACAGAGTGTGAGGTTTACAAATTTACTGGTGCTGGAGGCATAGCATTGTCCATGTATAAGACGGATGAATCTATCCGTGCTTTTGCTGAGGCTTCCATGAACACTGCCTACCAGAAAAAGTGGCCACTTTATCTTAGCGCTAAAAATACTACTCTGAAGAAATATGATGGAAGGTTCAAGGACATCGTCCAGGAAGCTTATGAAGCTCATTGGAGATCAAGATATGAAGCTGCAGGGATTTGGACAGATAACTTGTCATCCAAGCTGATTAGTTTGTATGAGCATCGTCTTATTGACGACATGGTTGCTTATGCTCTCAAAAGTGAAGGAGGTTATGTTTGGGCATGCAAAAATTATGATGGGGATGTGCAAAGTGACTTCTTAGCCCAAGGTTTTGGATCTCTGGGGCTGATGACATTTATACTGGTGTGCCGAGATGGAAAGACCATCGAAGCTGAAGCAGCCCATGGTACAGTAACCTGCCATTACACGGTCCACCAGAAAGGAGGCGAAACAAGCACAAACAGTATAGCTTCGATCTTTGCATGGTCACGAGGTCTTGCACACAGGGCAAAGTTAGATGGCAATGCCAGACTCTTAGATTTTACAGAGAAACTTGAAGCATCTTGCGTAGGAACTGTCGAATCTGGGAAGATGACCAAGGATATTGCACTCCTCATCCAAGGGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146
Pfam Domains
Protein Families
Iso_dh

Protein Analysis

344

Amino Acids

38.17

Weight (kDa)

8.42

Isoelectric Point (pI)

30.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 13 - 338 4.9e-51 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 827
AclWI GGATC 1 cut(s) 745
AcoI YGGCCR 1 cut(s) 461
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 2 cut(s) 515, 816
AfaI GTAC 5 cut(s) 38, 154, 263, 318, 811
AfeI AGCGCT 1 cut(s) 478
AfiI CCNNNNNNNGG 1 cut(s) 829
AgsI TTSAA 4 cut(s) 97, 103, 517, 959
AjnI CCWGG 3 cut(s) 200, 292, 528
AluBI AGCT 6 cut(s) 536, 545, 569, 604, 794, 872
AluI AGCT 6 cut(s) 536, 545, 569, 604, 794, 872
Alw26I GTCTC 1 cut(s) 235
AlwI GGATC 1 cut(s) 745
Aor51HI AGCGCT 1 cut(s) 478
AoxI GGCC 3 cut(s) 326, 461, 1026
ApaI GGGCCC 1 cut(s) 330
ApeKI GCWGC 2 cut(s) 569, 800
ApoI RAATTY 1 cut(s) 359
Asp700I GAANNNNTTC 2 cut(s) 92, 171
AspLEI GCGC 1 cut(s) 479
AspS9I GGNCC 6 cut(s) 129, 290, 326, 327, 832, 1026
AvaII GGWCC 3 cut(s) 129, 290, 832
BaeGI GKGCMC 1 cut(s) 330
BalI TGGCCA 1 cut(s) 463
BanII GRGCYC 1 cut(s) 330
BauI CACGAG 2 cut(s) 81, 891
BbsI GAAGAC 1 cut(s) 344
BbvCI CCTCAGC 1 cut(s) 426
BbvI GCAGC 2 cut(s) 556, 812
BccI CCATC 6 cut(s) 317, 500, 692, 770, 794, 914
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BciT130I CCWGG 3 cut(s) 202, 294, 530
BcoDI GTCTC 1 cut(s) 235
BfmI CTRYAG 2 cut(s) 176, 570
BfoI RGCGCY 1 cut(s) 480
BfuAI ACCTGC 1 cut(s) 827
BisI GCNGC 2 cut(s) 570, 801
BlsI GCNGC 2 cut(s) 571, 802
Bme1390I CCNGG 3 cut(s) 202, 294, 530
Bme18I GGWCC 3 cut(s) 129, 290, 832
BmgT120I GGNCC 6 cut(s) 129, 290, 326, 327, 832, 1026
BmiI GGNNCC 3 cut(s) 130, 328, 1027
BmrFI CCNGG 3 cut(s) 202, 294, 530
BmsI GCATC 3 cut(s) 265, 631, 971
BpiI GAAGAC 1 cut(s) 344
BpmI CTGGAG 2 cut(s) 54, 393
Bpu10I CCTNAGC 1 cut(s) 426
BsaJI CCNNGG 4 cut(s) 727, 805, 999, 1021
BsaXI ACNNNNNCTCC 4 cut(s) 52, 82, 660, 690
Bsc4I CCNNNNNNNGG 1 cut(s) 829
Bse1I ACTGG 2 cut(s) 370, 768
Bse3DI GCAATG 1 cut(s) 931
BseBI CCWGG 3 cut(s) 202, 294, 530
BseDI CCNNGG 4 cut(s) 727, 805, 999, 1021
BseGI GGATG 4 cut(s) 409, 596, 709, 1017
BseLI CCNNNNNNNGG 1 cut(s) 829
BseMI GCAATG 1 cut(s) 931
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 2 cut(s) 370, 768
BseRI GAGGAG 2 cut(s) 130, 1004
BseSI GKGCMC 1 cut(s) 330
BseXI GCAGC 2 cut(s) 556, 812
BshFI GGCC 3 cut(s) 328, 463, 1028
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 1 cut(s) 829
BsmAI GTCTC 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 142
BsnI GGCC 3 cut(s) 328, 463, 1028
Bsp120I GGGCCC 1 cut(s) 326
Bsp1286I GDGCHC 1 cut(s) 330
Bsp1407I TGTACA 1 cut(s) 36
Bsp143I GATC 3 cut(s) 554, 737, 876
Bsp19I CCATGG 1 cut(s) 805
BspANI GGCC 3 cut(s) 328, 463, 1028
BspCNI CTCAG 1 cut(s) 418
BspLI GGNNCC 3 cut(s) 130, 328, 1027
BspMAI CTGCAG 2 cut(s) 180, 574
BspMI ACCTGC 1 cut(s) 827
BspPI GGATC 1 cut(s) 745
BsrDI GCAATG 1 cut(s) 931
BsrGI TGTACA 1 cut(s) 36
BsrI ACTGG 2 cut(s) 370, 768
BssECI CCNNGG 4 cut(s) 727, 805, 999, 1021
BssMI GATC 3 cut(s) 554, 737, 876
BssSI CACGAG 2 cut(s) 81, 891
BssT1I CCWWGG 4 cut(s) 727, 805, 999, 1021
Bst2BI CACGAG 2 cut(s) 81, 891
Bst2UI CCWGG 3 cut(s) 202, 294, 530
Bst4CI ACNGT 5 cut(s) 157, 814, 832, 866, 979
BstAPI GCANNNNNTGC 1 cut(s) 275
BstAUI TGTACA 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 685
BstDEI CTNAG 4 cut(s) 426, 473, 721, 937
BstDSI CCRYGG 1 cut(s) 805
BstF5I GGATG 4 cut(s) 409, 596, 709, 1017
BstH2I RGCGCY 1 cut(s) 480
BstHHI GCGC 1 cut(s) 479
BstKTI GATC 3 cut(s) 557, 740, 879
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 3 cut(s) 554, 737, 876
BstMWI GCNNNNNNNGC 5 cut(s) 275, 542, 800, 855, 908
BstNI CCWGG 3 cut(s) 202, 294, 530
BstNSI RCATGY 2 cut(s) 248, 687
BstSCI CCNGG 3 cut(s) 200, 292, 528
BstSFI CTRYAG 2 cut(s) 176, 570
BstSLI GKGCMC 1 cut(s) 330
BstV1I GCAGC 2 cut(s) 556, 812
BstV2I GAAGAC 1 cut(s) 344
BstX2I RGATCY 1 cut(s) 737
BstXI CCANNNNNNTGG 1 cut(s) 734
BstYI RGATCY 1 cut(s) 737
BsuRI GGCC 3 cut(s) 328, 463, 1028
BtgI CCRYGG 1 cut(s) 805
BtsCI GGATG 4 cut(s) 409, 596, 709, 1017
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 1 cut(s) 441
BveI ACCTGC 1 cut(s) 827
Cac8I GCNNGC 1 cut(s) 685
CfoI GCGC 1 cut(s) 479
Cfr13I GGNCC 6 cut(s) 129, 290, 326, 327, 832, 1026
Csp6I GTAC 5 cut(s) 37, 153, 262, 317, 810
CviAII CATG 7 cut(s) 245, 391, 436, 640, 684, 806, 885
CviQI GTAC 5 cut(s) 37, 153, 262, 317, 810
DdeI CTNAG 4 cut(s) 426, 473, 721, 937
DpnI GATC 3 cut(s) 556, 739, 878
DpnII GATC 3 cut(s) 554, 737, 876
DraI TTTAAA 1 cut(s) 147
EaeI YGGCCR 1 cut(s) 461
Eco130I CCWWGG 4 cut(s) 727, 805, 999, 1021
Eco24I GRGCYC 1 cut(s) 330
Eco47I GGWCC 3 cut(s) 129, 290, 832
Eco47III AGCGCT 1 cut(s) 478
Eco57I CTGAAG 2 cut(s) 515, 816
EcoO109I RGGNCCY 3 cut(s) 290, 327, 1026
EcoRII CCWGG 3 cut(s) 200, 292, 528
EcoT14I CCWWGG 4 cut(s) 727, 805, 999, 1021
EcoT22I ATGCAT 1 cut(s) 236
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 4 cut(s) 727, 805, 999, 1021
FaeI CATG 7 cut(s) 248, 394, 439, 643, 687, 809, 888
FaqI GGGAC 1 cut(s) 142
FatI CATG 7 cut(s) 244, 390, 435, 639, 683, 805, 884
Fnu4HI GCNGC 2 cut(s) 570, 801
FokI GGATG 4 cut(s) 416, 583, 716, 1004
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 2 cut(s) 570, 801
GlaI GCGC 1 cut(s) 478
GluI GCNGC 2 cut(s) 570, 801
GsuI CTGGAG 2 cut(s) 54, 393
HaeII RGCGCY 1 cut(s) 480
HaeIII GGCC 3 cut(s) 328, 463, 1028
HhaI GCGC 1 cut(s) 479
Hin1II CATG 7 cut(s) 248, 394, 439, 643, 687, 809, 888
Hin6I GCGC 1 cut(s) 477
HinP1I GCGC 1 cut(s) 477
HindIII AAGCTT 1 cut(s) 534
HinfI GANTC 4 cut(s) 118, 407, 933, 983
Hpy166II GTNNAC 2 cut(s) 355, 835
Hpy188I TCNGA 1 cut(s) 495
Hpy188III TCNNGA 3 cut(s) 71, 558, 891
Hpy8I GTNNAC 2 cut(s) 355, 835
HpyAV CCTTC 4 cut(s) 82, 281, 504, 659
HpyCH4III ACNGT 5 cut(s) 157, 814, 832, 866, 979
HpyF10VI GCNNNNNNNGC 5 cut(s) 275, 542, 800, 855, 908
HpyF3I CTNAG 4 cut(s) 426, 473, 721, 937
Hsp92II CATG 7 cut(s) 248, 394, 439, 643, 687, 809, 888
HspAI GCGC 1 cut(s) 477
Kzo9I GATC 3 cut(s) 554, 737, 876
Lsp1109I GCAGC 2 cut(s) 556, 812
LweI GCATC 3 cut(s) 265, 631, 971
MaeIII GTNAC 3 cut(s) 713, 814, 888
MalI GATC 3 cut(s) 556, 739, 878
MboI GATC 3 cut(s) 554, 737, 876
MboII GAAGA 3 cut(s) 349, 508, 1003
MflI RGATCY 1 cut(s) 737
MhlI GDGCHC 1 cut(s) 330
MlsI TGGCCA 1 cut(s) 463
MluCI AATT 5 cut(s) 12, 31, 305, 359, 691
MluNI TGGCCA 1 cut(s) 463
MlyI GAGTC 2 cut(s) 127, 927
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 8 cut(s) 108, 343, 368, 421, 662, 839, 887, 1025
Mox20I TGGCCA 1 cut(s) 463
Mph1103I ATGCAT 1 cut(s) 236
MroXI GAANNNNTTC 2 cut(s) 92, 171
MscI TGGCCA 1 cut(s) 463
MseI TTAA 2 cut(s) 146, 303
Msp20I TGGCCA 1 cut(s) 463
MspR9I CCNGG 3 cut(s) 202, 294, 530
MvaI CCWGG 3 cut(s) 202, 294, 530
MwoI GCNNNNNNNGC 5 cut(s) 275, 542, 800, 855, 908
NcoI CCATGG 1 cut(s) 805
NdeII GATC 3 cut(s) 554, 737, 876
NlaIII CATG 7 cut(s) 248, 394, 439, 643, 687, 809, 888
NlaIV GGNNCC 3 cut(s) 130, 328, 1027
NmeAIII GCCGAG 1 cut(s) 797
NmuCI GTSAC 2 cut(s) 713, 888
NsiI ATGCAT 1 cut(s) 236
NspI RCATGY 2 cut(s) 248, 687
PaeI GCATGC 1 cut(s) 687
PdmI GAANNNNTTC 2 cut(s) 92, 171
PfeI GAWTC 2 cut(s) 407, 983
PflFI GACNNNGTC 1 cut(s) 524
PfoI TCCNGGA 1 cut(s) 528
PkrI GCNGC 2 cut(s) 571, 802
PleI GAGTC 2 cut(s) 126, 927
PpsI GAGTC 2 cut(s) 126, 927
PpuMI RGGWCCY 1 cut(s) 290
Psp5II RGGWCCY 1 cut(s) 290
Psp6I CCWGG 3 cut(s) 200, 292, 528
PspGI CCWGG 3 cut(s) 200, 292, 528
PspN4I GGNNCC 3 cut(s) 130, 328, 1027
PspOMI GGGCCC 1 cut(s) 326
PspPI GGNCC 6 cut(s) 129, 290, 326, 327, 832, 1026
PspPPI RGGWCCY 1 cut(s) 290
PsrI GAACNNNNNNTAC 2 cut(s) 431, 463
PstI CTGCAG 2 cut(s) 180, 574
PsuI RGATCY 1 cut(s) 737
PsyI GACNNNGTC 1 cut(s) 524
RsaI GTAC 5 cut(s) 38, 154, 263, 318, 811
RsaNI GTAC 5 cut(s) 37, 153, 262, 317, 810
SaqAI TTAA 2 cut(s) 146, 303
SatI GCNGC 2 cut(s) 570, 801
Sau3AI GATC 3 cut(s) 554, 737, 876
Sau96I GGNCC 6 cut(s) 129, 290, 326, 327, 832, 1026
SchI GAGTC 2 cut(s) 127, 927
ScrFI CCNGG 3 cut(s) 202, 294, 530
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 3 cut(s) 265, 631, 971
SfcI CTRYAG 2 cut(s) 176, 570
SinI GGWCC 3 cut(s) 129, 290, 832
SphI GCATGC 1 cut(s) 687
Sse9I AATT 5 cut(s) 12, 31, 305, 359, 691
StyD4I CCNGG 3 cut(s) 200, 292, 528
StyI CCWWGG 4 cut(s) 727, 805, 999, 1021
TaaI ACNGT 5 cut(s) 157, 814, 832, 866, 979
TaqI TCGA 3 cut(s) 789, 875, 981
TasI AATT 5 cut(s) 12, 31, 305, 359, 691
TatI WGTACW 1 cut(s) 36
TfiI GAWTC 2 cut(s) 407, 983
Tru1I TTAA 2 cut(s) 146, 303
Tru9I TTAA 2 cut(s) 146, 303
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 2 cut(s) 713, 888
TseI GCWGC 2 cut(s) 569, 800
Tsp45I GTSAC 2 cut(s) 713, 888
TspDTI ATGAA 5 cut(s) 90, 420, 452, 555, 579
TspGWI ACGGA 3 cut(s) 151, 404, 416
TspRI CASTG 1 cut(s) 448
Tth111I GACNNNGTC 1 cut(s) 524
VpaK11BI GGWCC 3 cut(s) 129, 290, 832
XapI RAATTY 1 cut(s) 359
XceI RCATGY 2 cut(s) 248, 687
XmnI GAANNNNTTC 2 cut(s) 92, 171
Zsp2I ATGCAT 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.