Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0001254

Physical Location & Seq

Genomic Coordinates Forward (+)

67618085 .. 67635590

Gene Structure 17506 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG460100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGATGCATTGTTTCCATGATGCAGGAGATGAAATGACTCGAATTTTCTGGAAATCTATAAAAGATAAGCTTATTTTGCCCTTTCTGGAACTGGATATCAAGTACTTTGATCTTGGCCTCCCTAATCGTGATGCCACCAGTGATAGAGTCACAATTGAAAGTGCAGAAGCTACTCTTAAGTACAATGTAGCAATCAAGTGTGCAACTATAACTCCTGATGAAACTCGTGTTAAGGAGTTCAACTTGAAACAGATGTGGAGGAGTCCAAATGGGACAATTCGGAACATTTTAAATGGTACTGTTTTTAGAGAACCTATTATCTGCAGGAATGTTCCCCGCCTTGTTCCAGTACCTGATGGGTCCAATGAGAAGCAAGAATATGAGGTTTTCAACTTTACTGGTGCTGGAGGGGTAGCTCTGTCAATGTATTACACTGATGAGTCTATCCGATCCTTTGCGGATGCATCTATGAATACTGCCTACCAGAAAAAGTGGCCACTTTATCTTAGCACTAAAAATACTATTCTCAAGAAATATGATGGAAGATTCAAGGACATCTTCCAGGAAGTTTATGAAACTCATTGGAGATCTAGATTTGAAGCCTCATCGAAATATCCCTCCCCGACGGATATTATGTTGGCCATGACAAGGAAGAAGAGTCCATCTTTAGTCTGCAACAGAAAATGTCAAGGTGATGCTGATGGGTATGATTTAGTTGTTGTTGATGCCATGCATAAAGCAACCTATATTGGAATCTACACTGTACGTAAGATTCAATACGGTGAAGAGATCACATTGGACTACAACTCTATTAAGGAGAGTAAGGAAGAATATGAAGCATCAGATTGTTTGTGCGGCAGTCAAGTTTGCCGGGGAGCTGAGTACGCAAAACTACCTATTATTCCACCGTATCTACAACCCGGCAATCAGCAATTTACATATGGTGCAAATTTTGCATCTGCAGGAGCTGGTGCTCTGGTTGAAACTTTCCACGGTTTGGTGATAGACCTTAATTCTCAACTTTCAAATTTTGAGCGAGTTCATGAGTCATTGAGGAAGAATTTAGGGGATGAAGAAGCCAACACTTTGATATCAAGAGCTGTTTACTTATTTAGTGTCGGAGGCAACGATTACTCATACATATTCGGGACAAACTCCAGCATCCAGCATCCTCACAGGGAATTTGTAGGAACTGTTCTAGGCAACATAACTGCAGCGATCAAAGAAGTATACAACAAAGGAGGAAGAAAATTTGGGTTTCTTAACCTTGATCCTCTTGGTTGTACACCGTACTCAAGAGCACTTGAGAAGAAACAAAAAGATGGATGCTTTGAAGAGATTACAGCGTATGTAGAACTCCACAATGAAGAACTTCCCAAACTCCTTCAAAAGCTGGAGACTGAACTCAAGGGATTTAGATACTCGCTTTCCAACTATAATGAGTTTCTGAGTGATAGGATAAATAACCCTTCTAAATATGTTGTGGGCTGCAAATTCAGCAGCTATTCAGCAGCCCTTGCACCTCCATGCCCATCAAATACACCAAAAAACGCCTGCAAACATGAATCTAGAGCATATCAAATTTCAATTCTCCAGCCACTCATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005886 GO:0016020 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

535

Amino Acids

60.56

Weight (kDa)

7.1

Isoelectric Point (pI)

35.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	8 - 193	5e-24	Isocitrate/isopropylmalate dehydrogenase
Lipase_GDSL	PF00657	302 - 482	1.2e-14	GDSL-like Lipase/Acylhydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001254)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54340 AT1G54340
fragaria_vesca	FvH4_3g13770 FvH4_3g13770 FvH4_3g13770 FvH4_3g13770 FvH4_3g13770 FvH4_3g13770 FvH4_3g13770
malus_domestica	MD05G1241100.v1.1 MD10G1219500.v1.1
prunus_persica	Prupe.4G124500_v2.0.a1 Prupe.4G124500_v2.0.a1
pyrus_communis	pycom05g21730 pycom10g18520
rosa_chinensis	RchiOBHm_Chr1g0322671 RchiOBHm_Chr1g0349881 RchiOBHm_Chr3g0487801 RchiOBHm_Chr3g0492471 RchiOBHm_Chr3g0496261 RchiOBHm_Chr3g0496271 RchiOBHm_Chr5g0022951 RchiOBHm_Chr5g0057661
rosa_laevigata	RLG00000017604 RLG00000018107 RLG00000019194 RLG00000032684
rosa_multiflora	Rmu_co8372099.1_g000001 Rmu_sc0000303.1_g000020 Rmu_sc0002263.1_g000012 Rmu_sc0002675.1_g000002 Rmu_sc0012663.1_g000003
rosa_roxburghii	Rroxscaffold_1G00056380
rosa_rugosa	Rorug05G0075800 Rorug05G0075900
rosa_samantha	Rh1BG010100 Rh1CG086100 Rh1DG139600 Rh2AG460100 Rh2BG230500 Rh2DG286700 Rh3AG284700 Rh3AG304200 Rh3AG328100 Rh3CG325200 Rh3DG354500 Rh4AG076600 Rh4CG081500 Rh5AG162200 Rh5BG161400 Rh5CG176100 Rh5CG412000 Rh5DG162800 Rh6BG196700
rosa_wichuraiana	Rw5G014600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	997
AccI	GTMKAC	1 cut(s)	1230
AciI	CCGC	3 cut(s)	337, 458, 855
AclWI	GGATC	2 cut(s)	444, 1265
AcoI	YGGCCR	2 cut(s)	494, 639
AcsI	RAATTY	8 cut(s)	42, 949, 1027, 1060, 1181, 1250, 1493, 1581
AfaI	GTAC	8 cut(s)	104, 182, 298, 351, 765, 884, 1285, 1292
AfiI	CCNNNNNNNGG	2 cut(s)	648, 997
AflII	CTTAAG	1 cut(s)	176
AjnI	CCWGG	1 cut(s)	561
AluBI	AGCT	8 cut(s)	70, 170, 416, 878, 968, 1100, 1393, 1503
AluI	AGCT	8 cut(s)	70, 170, 416, 878, 968, 1100, 1393, 1503
Alw21I	GWGCWC	2 cut(s)	976, 1303
Alw26I	GTCTC	1 cut(s)	1391
AlwI	GGATC	2 cut(s)	444, 1265
AlwNI	CAGNNNCTG	2 cut(s)	353, 968
AoxI	GGCC	3 cut(s)	115, 494, 639
ApeKI	GCWGC	4 cut(s)	1214, 1488, 1500, 1511
ApoI	RAATTY	8 cut(s)	42, 949, 1027, 1060, 1181, 1250, 1493, 1581
Asp700I	GAANNNNTTC	1 cut(s)	1371
AspS9I	GGNCC	1 cut(s)	360
AsuC2I	CCSGG	2 cut(s)	872, 921
AsuHPI	GGTGA	3 cut(s)	704, 794, 1012
AvaII	GGWCC	1 cut(s)	360
BalI	TGGCCA	2 cut(s)	496, 641
BauI	CACGAG	1 cut(s)	225
Bbv12I	GWGCWC	2 cut(s)	976, 1303
BbvI	GCAGC	4 cut(s)	1226, 1475, 1512, 1523
BccI	CCATC	6 cut(s)	350, 533, 670, 695, 1316, 1540
BciT130I	CCWGG	1 cut(s)	563
BcnI	CCSGG	2 cut(s)	872, 921
BcoDI	GTCTC	1 cut(s)	1391
BfaI	CTAG	3 cut(s)	591, 1199, 1569
BfmI	CTRYAG	3 cut(s)	322, 960, 1212
BfrI	CTTAAG	1 cut(s)	176
BglII	AGATCT	1 cut(s)	587
BisI	GCNGC	5 cut(s)	856, 1215, 1489, 1501, 1512
BlsI	GCNGC	5 cut(s)	857, 1216, 1490, 1502, 1513
BmcAI	AGTACT	1 cut(s)	104
Bme1390I	CCNGG	3 cut(s)	563, 872, 921
Bme18I	GGWCC	1 cut(s)	360
BmgT120I	GGNCC	1 cut(s)	360
BmiI	GGNNCC	1 cut(s)	361
BmrFI	CCNGG	3 cut(s)	563, 872, 921
BpmI	CTGGAG	4 cut(s)	426, 1141, 1415, 1577
BpuEI	CTTGAG	4 cut(s)	512, 1279, 1325, 1391
BpuMI	CCSGG	2 cut(s)	872, 921
BsaAI	YACGTR	1 cut(s)	767
BsaJI	CCNNGG	2 cut(s)	871, 991
BsaXI	ACNNNNNCTCC	2 cut(s)	196, 226
Bsc4I	CCNNNNNNNGG	2 cut(s)	648, 997
Bse1I	ACTGG	4 cut(s)	96, 138, 347, 403
BseBI	CCWGG	1 cut(s)	563
BseDI	CCNNGG	2 cut(s)	871, 991
BseGI	GGATG	5 cut(s)	466, 1075, 1161, 1168, 1331
BseLI	CCNNNNNNNGG	2 cut(s)	648, 997
BseMII	CTCAG	2 cut(s)	870, 1439
BseNI	ACTGG	4 cut(s)	96, 138, 347, 403
BseRI	GAGGAG	1 cut(s)	274
BseXI	GCAGC	4 cut(s)	1226, 1475, 1512, 1523
BsgI	GTGCAG	1 cut(s)	183
BshFI	GGCC	3 cut(s)	117, 496, 641
BsiHKAI	GWGCWC	2 cut(s)	976, 1303
BsiSI	CCGG	2 cut(s)	871, 921
BslFI	GGGAC	2 cut(s)	286, 1162
BslI	CCNNNNNNNGG	2 cut(s)	648, 997
BsmAI	GTCTC	1 cut(s)	1391
BsmFI	GGGAC	2 cut(s)	286, 1162
BsnI	GGCC	3 cut(s)	117, 496, 641
Bsp1286I	GDGCHC	2 cut(s)	976, 1303
Bsp1407I	TGTACA	1 cut(s)	1283
Bsp143I	GATC	6 cut(s)	109, 449, 587, 789, 1218, 1270
BspACI	CCGC	3 cut(s)	337, 458, 855
BspANI	GGCC	3 cut(s)	117, 496, 641
BspCNI	CTCAG	2 cut(s)	871, 1440
BspHI	TCATGA	1 cut(s)	1042
BspLI	GGNNCC	1 cut(s)	361
BspMAI	CTGCAG	3 cut(s)	326, 964, 1216
BspPI	GGATC	2 cut(s)	444, 1265
BspTI	CTTAAG	1 cut(s)	176
BsrGI	TGTACA	1 cut(s)	1283
BsrI	ACTGG	4 cut(s)	96, 138, 347, 403
BssECI	CCNNGG	2 cut(s)	871, 991
BssMI	GATC	6 cut(s)	109, 449, 587, 789, 1218, 1270
BssNAI	GTATAC	1 cut(s)	1231
BssSI	CACGAG	1 cut(s)	225
Bst1107I	GTATAC	1 cut(s)	1231
Bst2BI	CACGAG	1 cut(s)	225
Bst2UI	CCWGG	1 cut(s)	563
Bst4CI	ACNGT	7 cut(s)	301, 763, 782, 909, 995, 1195, 1290
Bst6I	CTCTTC	3 cut(s)	650, 780, 1329
BstAFI	CTTAAG	1 cut(s)	176
BstAPI	GCANNNNNTGC	2 cut(s)	953, 1517
BstAUI	TGTACA	1 cut(s)	1283
BstBAI	YACGTR	1 cut(s)	767
BstC8I	GCNNGC	1 cut(s)	1555
BstDEI	CTNAG	3 cut(s)	506, 879, 1448
BstDSI	CCRYGG	1 cut(s)	991
BstF5I	GGATG	5 cut(s)	466, 1075, 1161, 1168, 1331
BstKTI	GATC	6 cut(s)	112, 452, 590, 792, 1221, 1273
BstMAI	GTCTC	1 cut(s)	1391
BstMBI	GATC	6 cut(s)	109, 449, 587, 789, 1218, 1270
BstMWI	GCNNNNNNNGC	5 cut(s)	76, 884, 953, 1497, 1517
BstNI	CCWGG	1 cut(s)	563
BstSCI	CCNGG	3 cut(s)	561, 870, 919
BstSFI	CTRYAG	3 cut(s)	322, 960, 1212
BstSNI	TACGTA	1 cut(s)	767
BstV1I	GCAGC	4 cut(s)	1226, 1475, 1512, 1523
BstX2I	RGATCY	1 cut(s)	587
BstYI	RGATCY	1 cut(s)	587
BstZ17I	GTATAC	1 cut(s)	1231
BsuRI	GGCC	3 cut(s)	117, 496, 641
BtgI	CCRYGG	1 cut(s)	991
BtsCI	GGATG	5 cut(s)	466, 1075, 1161, 1168, 1331
BtsIMutI	CAGTG	3 cut(s)	145, 432, 759
Cac8I	GCNNGC	1 cut(s)	1555
CaiI	CAGNNNCTG	2 cut(s)	353, 968
CciI	TCATGA	1 cut(s)	1042
Cfr13I	GGNCC	1 cut(s)	360
Csp6I	GTAC	8 cut(s)	103, 181, 297, 350, 764, 883, 1284, 1291
CviAII	CATG	6 cut(s)	17, 643, 730, 1043, 1527, 1562
CviQI	GTAC	8 cut(s)	103, 181, 297, 350, 764, 883, 1284, 1291
DdeI	CTNAG	3 cut(s)	506, 879, 1448
DpnI	GATC	6 cut(s)	111, 451, 589, 791, 1220, 1272
DpnII	GATC	6 cut(s)	109, 449, 587, 789, 1218, 1270
DraI	TTTAAA	1 cut(s)	291
EaeI	YGGCCR	2 cut(s)	494, 639
Eam1104I	CTCTTC	3 cut(s)	650, 780, 1329
EarI	CTCTTC	3 cut(s)	650, 780, 1329
Eco105I	TACGTA	1 cut(s)	767
Eco32I	GATATC	2 cut(s)	97, 1092
Eco47I	GGWCC	1 cut(s)	360
EcoRII	CCWGG	1 cut(s)	561
EcoRV	GATATC	2 cut(s)	97, 1092
EcoT22I	ATGCAT	3 cut(s)	9, 466, 735
FaeI	CATG	6 cut(s)	20, 646, 733, 1046, 1530, 1565
FalI	AAGNNNNNCTT	6 cut(s)	54, 86, 159, 191, 542, 574
FaqI	GGGAC	2 cut(s)	286, 1162
FatI	CATG	6 cut(s)	16, 642, 729, 1042, 1526, 1561
FauI	CCCGC	1 cut(s)	344
FauNDI	CATATG	1 cut(s)	940
FblI	GTMKAC	1 cut(s)	1230
Fnu4HI	GCNGC	5 cut(s)	856, 1215, 1489, 1501, 1512
FokI	GGATG	5 cut(s)	473, 1082, 1148, 1155, 1338
Fsp4HI	GCNGC	5 cut(s)	856, 1215, 1489, 1501, 1512
FspBI	CTAG	3 cut(s)	591, 1199, 1569
GluI	GCNGC	5 cut(s)	856, 1215, 1489, 1501, 1512
GsuI	CTGGAG	4 cut(s)	426, 1141, 1415, 1577
HaeIII	GGCC	3 cut(s)	117, 496, 641
HapII	CCGG	2 cut(s)	871, 921
Hin1II	CATG	6 cut(s)	20, 646, 733, 1046, 1530, 1565
HindIII	AAGCTT	1 cut(s)	68
HpaII	CCGG	2 cut(s)	871, 921
HphI	GGTGA	3 cut(s)	704, 794, 1012
Hpy166II	GTNNAC	3 cut(s)	1105, 1231, 1286
Hpy188I	TCNGA	5 cut(s)	282, 449, 844, 1121, 1449
Hpy8I	GTNNAC	3 cut(s)	1105, 1231, 1286
Hpy99I	CGWCG	1 cut(s)	628
HpyAV	CCTTC	2 cut(s)	1394, 1479
HpyCH4III	ACNGT	7 cut(s)	301, 763, 782, 909, 995, 1195, 1290
HpyCH4IV	ACGT	1 cut(s)	766
HpyF10VI	GCNNNNNNNGC	5 cut(s)	76, 884, 953, 1497, 1517
HpyF3I	CTNAG	3 cut(s)	506, 879, 1448
HpySE526I	ACGT	1 cut(s)	766
Hsp92II	CATG	6 cut(s)	20, 646, 733, 1046, 1530, 1565
Kzo9I	GATC	6 cut(s)	109, 449, 587, 789, 1218, 1270
LmnI	GCTCC	2 cut(s)	875, 965
Lsp1109I	GCAGC	4 cut(s)	1226, 1475, 1512, 1523
MaeI	CTAG	3 cut(s)	591, 1199, 1569
MaeII	ACGT	1 cut(s)	766
MaeIII	GTNAC	1 cut(s)	148
MalI	GATC	6 cut(s)	111, 451, 589, 791, 1220, 1272
MboI	GATC	6 cut(s)	109, 449, 587, 789, 1218, 1270
MfeI	CAATTG	1 cut(s)	153
MflI	RGATCY	1 cut(s)	587
MhlI	GDGCHC	2 cut(s)	976, 1303
MlsI	TGGCCA	2 cut(s)	496, 641
MluNI	TGGCCA	2 cut(s)	496, 641
MlyI	GAGTC	6 cut(s)	31, 156, 271, 449, 667, 1055
MmeI	TCCRAC	2 cut(s)	1099, 1455
Mox20I	TGGCCA	2 cut(s)	496, 641
Mph1103I	ATGCAT	3 cut(s)	9, 466, 735
MroXI	GAANNNNTTC	1 cut(s)	1371
MscI	TGGCCA	2 cut(s)	496, 641
MseI	TTAA	7 cut(s)	177, 231, 290, 813, 1011, 1263, 1606
MslI	CAYNNNNRTG	1 cut(s)	1525
Msp20I	TGGCCA	2 cut(s)	496, 641
MspCI	CTTAAG	1 cut(s)	176
MspI	CCGG	2 cut(s)	871, 921
MspR9I	CCNGG	3 cut(s)	563, 872, 921
MunI	CAATTG	1 cut(s)	153
MvaI	CCWGG	1 cut(s)	563
MwoI	GCNNNNNNNGC	5 cut(s)	76, 884, 953, 1497, 1517
NciI	CCSGG	2 cut(s)	872, 921
NdeI	CATATG	1 cut(s)	940
NdeII	GATC	6 cut(s)	109, 449, 587, 789, 1218, 1270
NlaIII	CATG	6 cut(s)	20, 646, 733, 1046, 1530, 1565
NlaIV	GGNNCC	1 cut(s)	361
NmuCI	GTSAC	1 cut(s)	148
NsiI	ATGCAT	3 cut(s)	9, 466, 735
PagI	TCATGA	1 cut(s)	1042
PcsI	WCGNNNNNNNCGW	1 cut(s)	1125
PdmI	GAANNNNTTC	1 cut(s)	1371
PfeI	GAWTC	4 cut(s)	546, 753, 772, 1565
PflMI	CCANNNNNTGG	1 cut(s)	997
PfoI	TCCNGGA	1 cut(s)	561
PkrI	GCNGC	5 cut(s)	857, 1216, 1490, 1502, 1513
PleI	GAGTC	6 cut(s)	31, 155, 270, 448, 666, 1054
PpsI	GAGTC	6 cut(s)	31, 155, 270, 448, 666, 1054
Ppu21I	YACGTR	1 cut(s)	767
Psp6I	CCWGG	1 cut(s)	561
PspGI	CCWGG	1 cut(s)	561
PspN4I	GGNNCC	1 cut(s)	361
PspPI	GGNCC	1 cut(s)	360
PsrI	GAACNNNNNNTAC	2 cut(s)	1179, 1211
PstI	CTGCAG	3 cut(s)	326, 964, 1216
PstNI	CAGNNNCTG	2 cut(s)	353, 968
PsuI	RGATCY	1 cut(s)	587
RsaI	GTAC	8 cut(s)	104, 182, 298, 351, 765, 884, 1285, 1292
RsaNI	GTAC	8 cut(s)	103, 181, 297, 350, 764, 883, 1284, 1291
RseI	CAYNNNNRTG	1 cut(s)	1525
SaqAI	TTAA	7 cut(s)	177, 231, 290, 813, 1011, 1263, 1606
SatI	GCNGC	5 cut(s)	856, 1215, 1489, 1501, 1512
Sau3AI	GATC	6 cut(s)	109, 449, 587, 789, 1218, 1270
Sau96I	GGNCC	1 cut(s)	360
ScaI	AGTACT	1 cut(s)	104
SchI	GAGTC	6 cut(s)	31, 156, 271, 449, 667, 1055
ScrFI	CCNGG	3 cut(s)	563, 872, 921
SduI	GDGCHC	2 cut(s)	976, 1303
SfcI	CTRYAG	3 cut(s)	322, 960, 1212
SinI	GGWCC	1 cut(s)	360
SmiMI	CAYNNNNRTG	1 cut(s)	1525
SmlI	CTYRAG	5 cut(s)	176, 527, 1294, 1304, 1406
SmoI	CTYRAG	5 cut(s)	176, 527, 1294, 1304, 1406
SnaBI	TACGTA	1 cut(s)	767
SsiI	CCGC	3 cut(s)	337, 458, 855
SspMI	CTAG	3 cut(s)	591, 1199, 1569
StyD4I	CCNGG	3 cut(s)	561, 870, 919
TaaI	ACNGT	7 cut(s)	301, 763, 782, 909, 995, 1195, 1290
TaiI	ACGT	1 cut(s)	769
TaqI	TCGA	2 cut(s)	40, 608
TatI	WGTACW	3 cut(s)	102, 180, 1283
TauI	GCSGC	1 cut(s)	858
TfiI	GAWTC	4 cut(s)	546, 753, 772, 1565
Tru1I	TTAA	7 cut(s)	177, 231, 290, 813, 1011, 1263, 1606
Tru9I	TTAA	7 cut(s)	177, 231, 290, 813, 1011, 1263, 1606
TscAI	CASTG	3 cut(s)	145, 439, 766
TseFI	GTSAC	1 cut(s)	148
TseI	GCWGC	4 cut(s)	1214, 1488, 1500, 1511
Tsp45I	GTSAC	1 cut(s)	148
TspDTI	ATGAA	9 cut(s)	45, 234, 485, 588, 849, 1031, 1086, 1380, 1578
TspGWI	ACGGA	1 cut(s)	641
TspRI	CASTG	3 cut(s)	145, 439, 766
Van91I	CCANNNNNTGG	1 cut(s)	997
Vha464I	CTTAAG	1 cut(s)	176
VpaK11BI	GGWCC	1 cut(s)	360
XapI	RAATTY	8 cut(s)	42, 949, 1027, 1060, 1181, 1250, 1493, 1581
XbaI	TCTAGA	2 cut(s)	590, 1568
XmiI	GTMKAC	1 cut(s)	1230
XmnI	GAANNNNTTC	1 cut(s)	1371
XspI	CTAG	3 cut(s)	591, 1199, 1569
ZrmI	AGTACT	1 cut(s)	104
Zsp2I	ATGCAT	3 cut(s)	9, 466, 735

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.