Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0001254

Physical Location & Seq

Genomic Coordinates Reverse (-)

36944395 .. 36948919

Gene Structure 4525 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG196700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 858 bp

ATGGTAGGAAGAAATAGAGAGGCCAGAAGGATTGAAGGAGATGAAATGACTCGAATTTTCTTGACATCTATAAAAGATAAGGTAATTGTGATATTCATGGCATCAGGTGATTTCATCATTTCATGTTCACAGCTTATTTTGCCCTTTCTGGAACTGGATATCAAGTACTTTGATCTTGGCCTCCCTAATCATGATGCTACCAGTGATAGAGTCACAATTGAAAGTGCAGAAGCTACTCTTAAGTACAATGTAGCAATCAAGTGTGCAACTATAACTCCTGATGAAACTCGTGTTAAGGAGTTCAACTTGAAACAGATGTGGAGGAGTCCAAATGGGACAATCCGGAACATTTTAAATGGTACTGTTTTTAGAGAACTTATTATCTGCAGAAATGTTCCCCGCCTTGTTCCAGTACCTGATGGGTCCAATGAGAAGCAAGAATATGAGGTTTTCAACTTTACTGGTGCTGGAGGGGTAGCTCTGTCAATGTATAACACTGATGAGTCTATCCGATCCTTTGCGGATGCATCTATGAATACTGCCTACCAGAAAAAGTGGCCACTTTATCTTAGCACTAAAAATACTATTCTCAAGAAATATGATGGAAGATTCAAGGACATCTTCCAGGAAGTTTATGAAACTCATTGGAGATCTAGATTTGAAGCCTCATCGAAATATCCCTCCCCGACGGATATTATGTTGGCCATGACAAGGAAGAAGAGTCCATCTTTAGTCTGCAACAGAAAATGTCAGGGTGATGCTGATGGGTATGATTTAGTTGTTGTTGATGCCATGCATAAAGCAACCTATGCAAGTAGAATTTGTCACTCGTGCCGACCTAATTGTGAAGCAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005886 GO:0016020 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146

Pfam Domains

Protein Families

Iso_dh

Protein Analysis

285

Amino Acids

32.49

Weight (kDa)

8.68

Isoelectric Point (pI)

42.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Iso_dh	PF00180	48 - 214	1.2e-20	Isocitrate/isopropylmalate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0001254)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54340 AT1G54340
fragaria_vesca	FvH4_3g13770 FvH4_3g13770 FvH4_3g13770 FvH4_3g13770 FvH4_3g13770 FvH4_3g13770 FvH4_3g13770
malus_domestica	MD05G1241100.v1.1 MD10G1219500.v1.1
prunus_persica	Prupe.4G124500_v2.0.a1 Prupe.4G124500_v2.0.a1
pyrus_communis	pycom05g21730 pycom10g18520
rosa_chinensis	RchiOBHm_Chr1g0322671 RchiOBHm_Chr1g0349881 RchiOBHm_Chr3g0487801 RchiOBHm_Chr3g0492471 RchiOBHm_Chr3g0496261 RchiOBHm_Chr3g0496271 RchiOBHm_Chr5g0022951 RchiOBHm_Chr5g0057661
rosa_laevigata	RLG00000017604 RLG00000018107 RLG00000019194 RLG00000032684
rosa_multiflora	Rmu_co8372099.1_g000001 Rmu_sc0000303.1_g000020 Rmu_sc0002263.1_g000012 Rmu_sc0002675.1_g000002 Rmu_sc0012663.1_g000003
rosa_roxburghii	Rroxscaffold_1G00056380
rosa_rugosa	Rorug05G0075800 Rorug05G0075900
rosa_samantha	Rh1BG010100 Rh1CG086100 Rh1DG139600 Rh2AG460100 Rh2BG230500 Rh2DG286700 Rh3AG284700 Rh3AG304200 Rh3AG328100 Rh3CG325200 Rh3DG354500 Rh4AG076600 Rh4CG081500 Rh5AG162200 Rh5BG161400 Rh5CG176100 Rh5CG412000 Rh5DG162800 Rh6BG196700
rosa_wichuraiana	Rw5G014600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	1 cut(s)	342
AciI	CCGC	2 cut(s)	400, 521
AclWI	GGATC	1 cut(s)	507
AcoI	YGGCCR	2 cut(s)	557, 702
AcsI	RAATTY	2 cut(s)	54, 819
AfaI	GTAC	4 cut(s)	167, 245, 361, 414
AfiI	CCNNNNNNNGG	1 cut(s)	711
AflII	CTTAAG	1 cut(s)	239
AgsI	TTSAA	7 cut(s)	35, 221, 304, 310, 454, 613, 662
AjnI	CCWGG	1 cut(s)	624
AluBI	AGCT	3 cut(s)	133, 233, 479
AluI	AGCT	3 cut(s)	133, 233, 479
AlwI	GGATC	1 cut(s)	507
AlwNI	CAGNNNCTG	1 cut(s)	416
Aor13HI	TCCGGA	1 cut(s)	342
AoxI	GGCC	4 cut(s)	21, 178, 557, 702
ApoI	RAATTY	2 cut(s)	54, 819
AspS9I	GGNCC	1 cut(s)	423
AsuHPI	GGTGA	2 cut(s)	119, 767
AvaII	GGWCC	1 cut(s)	423
BalI	TGGCCA	2 cut(s)	559, 704
BauI	CACGAG	2 cut(s)	288, 829
BccI	CCATC	4 cut(s)	413, 596, 733, 758
BciT130I	CCWGG	1 cut(s)	626
BfaI	CTAG	1 cut(s)	654
BfmI	CTRYAG	1 cut(s)	385
BfrI	CTTAAG	1 cut(s)	239
BglII	AGATCT	1 cut(s)	650
BmcAI	AGTACT	1 cut(s)	167
Bme1390I	CCNGG	1 cut(s)	626
Bme18I	GGWCC	1 cut(s)	423
BmgT120I	GGNCC	1 cut(s)	423
BmiI	GGNNCC	1 cut(s)	424
BmrFI	CCNGG	1 cut(s)	626
BmsI	GCATC	6 cut(s)	110, 184, 514, 536, 748, 778
BpmI	CTGGAG	1 cut(s)	489
BpuEI	CTTGAG	1 cut(s)	575
BsaWI	WCCGGW	1 cut(s)	342
BsaXI	ACNNNNNCTCC	2 cut(s)	259, 289
Bsc4I	CCNNNNNNNGG	1 cut(s)	711
Bse1I	ACTGG	4 cut(s)	159, 201, 410, 466
BseAI	TCCGGA	1 cut(s)	342
BseBI	CCWGG	1 cut(s)	626
BseGI	GGATG	1 cut(s)	529
BseLI	CCNNNNNNNGG	1 cut(s)	711
BseNI	ACTGG	4 cut(s)	159, 201, 410, 466
BseRI	GAGGAG	1 cut(s)	337
BsgI	GTGCAG	1 cut(s)	246
BshFI	GGCC	4 cut(s)	23, 180, 559, 704
BsiSI	CCGG	1 cut(s)	343
BslFI	GGGAC	1 cut(s)	349
BslI	CCNNNNNNNGG	1 cut(s)	711
BsmFI	GGGAC	1 cut(s)	349
BsnI	GGCC	4 cut(s)	23, 180, 559, 704
Bsp13I	TCCGGA	1 cut(s)	342
Bsp143I	GATC	3 cut(s)	172, 512, 650
BspACI	CCGC	2 cut(s)	400, 521
BspANI	GGCC	4 cut(s)	23, 180, 559, 704
BspEI	TCCGGA	1 cut(s)	342
BspHI	TCATGA	1 cut(s)	190
BspLI	GGNNCC	1 cut(s)	424
BspMAI	CTGCAG	1 cut(s)	389
BspPI	GGATC	1 cut(s)	507
BspTI	CTTAAG	1 cut(s)	239
BsrI	ACTGG	4 cut(s)	159, 201, 410, 466
BssMI	GATC	3 cut(s)	172, 512, 650
BssSI	CACGAG	2 cut(s)	288, 829
Bst2BI	CACGAG	2 cut(s)	288, 829
Bst2UI	CCWGG	1 cut(s)	626
Bst4CI	ACNGT	1 cut(s)	364
Bst6I	CTCTTC	1 cut(s)	713
BstAFI	CTTAAG	1 cut(s)	239
BstAPI	GCANNNNNTGC	1 cut(s)	809
BstDEI	CTNAG	1 cut(s)	569
BstF5I	GGATG	1 cut(s)	529
BstKTI	GATC	3 cut(s)	175, 515, 653
BstMBI	GATC	3 cut(s)	172, 512, 650
BstMWI	GCNNNNNNNGC	2 cut(s)	139, 809
BstNI	CCWGG	1 cut(s)	626
BstSCI	CCNGG	1 cut(s)	624
BstSFI	CTRYAG	1 cut(s)	385
BstX2I	RGATCY	1 cut(s)	650
BstYI	RGATCY	1 cut(s)	650
BsuRI	GGCC	4 cut(s)	23, 180, 559, 704
BtsCI	GGATG	1 cut(s)	529
BtsIMutI	CAGTG	2 cut(s)	208, 495
CaiI	CAGNNNCTG	1 cut(s)	416
CciI	TCATGA	1 cut(s)	190
Cfr13I	GGNCC	1 cut(s)	423
Csp6I	GTAC	4 cut(s)	166, 244, 360, 413
CviAII	CATG	5 cut(s)	97, 123, 191, 706, 793
CviJI	RGCY	8 cut(s)	23, 133, 180, 233, 479, 559, 665, 704
CviKI_1	RGCY	8 cut(s)	23, 133, 180, 233, 479, 559, 665, 704
CviQI	GTAC	4 cut(s)	166, 244, 360, 413
DdeI	CTNAG	1 cut(s)	569
DpnI	GATC	3 cut(s)	174, 514, 652
DpnII	GATC	3 cut(s)	172, 512, 650
DraI	TTTAAA	1 cut(s)	354
EaeI	YGGCCR	2 cut(s)	557, 702
Eam1104I	CTCTTC	1 cut(s)	713
EarI	CTCTTC	1 cut(s)	713
Eco32I	GATATC	1 cut(s)	160
Eco47I	GGWCC	1 cut(s)	423
EcoRII	CCWGG	1 cut(s)	624
EcoRV	GATATC	1 cut(s)	160
EcoT22I	ATGCAT	2 cut(s)	529, 798
FaeI	CATG	5 cut(s)	100, 126, 194, 709, 796
FalI	AAGNNNNNCTT	4 cut(s)	222, 254, 605, 637
FaqI	GGGAC	1 cut(s)	349
FatI	CATG	5 cut(s)	96, 122, 190, 705, 792
FauI	CCCGC	1 cut(s)	407
FokI	GGATG	1 cut(s)	536
FspBI	CTAG	1 cut(s)	654
GsuI	CTGGAG	1 cut(s)	489
HaeIII	GGCC	4 cut(s)	23, 180, 559, 704
HapII	CCGG	1 cut(s)	343
Hin1II	CATG	5 cut(s)	100, 126, 194, 709, 796
HinfI	GANTC	6 cut(s)	49, 210, 325, 503, 609, 721
HpaII	CCGG	1 cut(s)	343
HphI	GGTGA	2 cut(s)	119, 767
Hpy166II	GTNNAC	1 cut(s)	128
Hpy188I	TCNGA	1 cut(s)	512
Hpy188III	TCNNGA	7 cut(s)	61, 149, 191, 278, 343, 592, 654
Hpy8I	GTNNAC	1 cut(s)	128
Hpy99I	CGWCG	1 cut(s)	691
HpyAV	CCTTC	2 cut(s)	21, 29
HpyCH4III	ACNGT	1 cut(s)	364
HpyCH4V	TGCA	7 cut(s)	227, 266, 387, 527, 738, 796, 812
HpyF10VI	GCNNNNNNNGC	2 cut(s)	139, 809
HpyF3I	CTNAG	1 cut(s)	569
Hsp92II	CATG	5 cut(s)	100, 126, 194, 709, 796
Kpn2I	TCCGGA	1 cut(s)	342
Kzo9I	GATC	3 cut(s)	172, 512, 650
LweI	GCATC	6 cut(s)	110, 184, 514, 536, 748, 778
MaeI	CTAG	1 cut(s)	654
MaeIII	GTNAC	2 cut(s)	211, 824
MalI	GATC	3 cut(s)	174, 514, 652
MboI	GATC	3 cut(s)	172, 512, 650
MboII	GAAGA	5 cut(s)	21, 613, 618, 727, 730
MfeI	CAATTG	1 cut(s)	216
MflI	RGATCY	1 cut(s)	650
MlsI	TGGCCA	2 cut(s)	559, 704
MluCI	AATT	5 cut(s)	54, 84, 216, 819, 841
MluNI	TGGCCA	2 cut(s)	559, 704
MlyI	GAGTC	5 cut(s)	43, 219, 334, 512, 730
MnlI	CCTC	7 cut(s)	13, 191, 315, 439, 464, 676, 691
Mox20I	TGGCCA	2 cut(s)	559, 704
Mph1103I	ATGCAT	2 cut(s)	529, 798
MroI	TCCGGA	1 cut(s)	342
MscI	TGGCCA	2 cut(s)	559, 704
MseI	TTAA	3 cut(s)	240, 294, 353
Msp20I	TGGCCA	2 cut(s)	559, 704
MspCI	CTTAAG	1 cut(s)	239
MspI	CCGG	1 cut(s)	343
MspR9I	CCNGG	1 cut(s)	626
MunI	CAATTG	1 cut(s)	216
MvaI	CCWGG	1 cut(s)	626
MwoI	GCNNNNNNNGC	2 cut(s)	139, 809
NdeII	GATC	3 cut(s)	172, 512, 650
NlaIII	CATG	5 cut(s)	100, 126, 194, 709, 796
NlaIV	GGNNCC	1 cut(s)	424
NmuCI	GTSAC	2 cut(s)	211, 824
NsiI	ATGCAT	2 cut(s)	529, 798
PagI	TCATGA	1 cut(s)	190
PfeI	GAWTC	1 cut(s)	609
PfoI	TCCNGGA	1 cut(s)	624
PleI	GAGTC	5 cut(s)	43, 218, 333, 511, 729
PpsI	GAGTC	5 cut(s)	43, 218, 333, 511, 729
Psp6I	CCWGG	1 cut(s)	624
PspGI	CCWGG	1 cut(s)	624
PspN4I	GGNNCC	1 cut(s)	424
PspPI	GGNCC	1 cut(s)	423
PstI	CTGCAG	1 cut(s)	389
PstNI	CAGNNNCTG	1 cut(s)	416
PsuI	RGATCY	1 cut(s)	650
RsaI	GTAC	4 cut(s)	167, 245, 361, 414
RsaNI	GTAC	4 cut(s)	166, 244, 360, 413
SaqAI	TTAA	3 cut(s)	240, 294, 353
Sau3AI	GATC	3 cut(s)	172, 512, 650
Sau96I	GGNCC	1 cut(s)	423
ScaI	AGTACT	1 cut(s)	167
SchI	GAGTC	5 cut(s)	43, 219, 334, 512, 730
ScrFI	CCNGG	1 cut(s)	626
SetI	ASST	9 cut(s)	84, 109, 135, 235, 418, 450, 481, 809, 841
SfaNI	GCATC	6 cut(s)	110, 184, 514, 536, 748, 778
SfcI	CTRYAG	1 cut(s)	385
SinI	GGWCC	1 cut(s)	423
SmlI	CTYRAG	2 cut(s)	239, 590
SmoI	CTYRAG	2 cut(s)	239, 590
Sse9I	AATT	5 cut(s)	54, 84, 216, 819, 841
SsiI	CCGC	2 cut(s)	400, 521
SspMI	CTAG	1 cut(s)	654
StyD4I	CCNGG	1 cut(s)	624
TaaI	ACNGT	1 cut(s)	364
TaqI	TCGA	2 cut(s)	52, 671
TasI	AATT	5 cut(s)	54, 84, 216, 819, 841
TatI	WGTACW	2 cut(s)	165, 243
TfiI	GAWTC	1 cut(s)	609
Tru1I	TTAA	3 cut(s)	240, 294, 353
Tru9I	TTAA	3 cut(s)	240, 294, 353
TscAI	CASTG	2 cut(s)	208, 502
TseFI	GTSAC	2 cut(s)	211, 824
Tsp45I	GTSAC	2 cut(s)	211, 824
TspDTI	ATGAA	7 cut(s)	57, 85, 103, 111, 297, 548, 651
TspGWI	ACGGA	1 cut(s)	704
TspRI	CASTG	2 cut(s)	208, 502
Vha464I	CTTAAG	1 cut(s)	239
VpaK11BI	GGWCC	1 cut(s)	423
XapI	RAATTY	2 cut(s)	54, 819
XbaI	TCTAGA	1 cut(s)	653
XspI	CTAG	1 cut(s)	654
ZrmI	AGTACT	1 cut(s)	167
Zsp2I	ATGCAT	2 cut(s)	529, 798

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.