Rmu_sc0012663.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0012663.1
OG0001254
Physical Location & Seq
Reverse (-)
1222 .. 4673
3452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0012663.1_g000003.1.cds

Sequence Viewer

Length: 1047 bp
atggctaccacctactccaatccttcaccaaactcctcctatggtggtcgtttgtttttgtctctatcgacttccactgttgcgcaacagcggctagctcagccctttgggcctttcactcttgcttgggttcaccgatcccaaccgcctgcattccggcttccgatccagccacctcaccggagcctcccagcctttgatctagcttctaagaccttggcgctgacgtggcgggcgaggtcgcggatcttggagggtgaaggtgaatcggaggacgaagatggtgaatcggaatctggctggggagaacgagttggtgaagaggaagaagcagtgagttggagtaggaggaaggcgaggaagaagaagcggaggtcgcagactcagatatcgccgacgacagtggtcggtgggtccgattcgaattccggcgagggctttccagaagcaaggggaggagagagccctattcctccctcggcatcaacatcatcagacccaaccacgcctccatcaatccctacatcgcctgcccccgtcactcattcccagcccatcaccttcaacgaggggccttctctcatccctgcaccagtttcgtcactgtctgcagcctaccgccatcgcccaacgactgcgtcggccatagtgtctgcgtcggccatagcgtctatctctcctttatctccgaccacagcgccgatctctcctccaatccagaccacagcgccgatagctcctccacccgatgctgcttcacatcccacttcgcttgccgaaccttcaactcctcctgcttgggttccattaccatcagccgttgcatctgtagctgaccctctatctctagtttcgtcgccatctgtggcttctcctccggtgcctgaaccgagccccccacctccggcccctgctgcgagcccaccttctccagctcctactgagagcccacctcctccacaccaccccatggtcacaggactccgagatggcactcaccaaaccagaattcgaactgatggcacgatcaggtaccccattccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

348

Amino Acids

36.25

Weight (kDa)

6.46

Isoelectric Point (pI)

80.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 84
Acc65I GGTACC 1 cut(s) 1032
AccB1I GGYRCC 2 cut(s) 880, 1032
AccB7I CCANNNNNTGG 1 cut(s) 970
AccII CGCG 1 cut(s) 244
AciI CCGC 6 cut(s) 91, 146, 232, 244, 370, 619
AclWI GGATC 3 cut(s) 132, 160, 254
AcoI YGGCCR 2 cut(s) 642, 660
AcsI RAATTY 2 cut(s) 424, 1008
AfaI GTAC 1 cut(s) 1034
AfiI CCNNNNNNNGG 2 cut(s) 904, 970
AgsI TTSAA 2 cut(s) 565, 786
AjiI CACGTC 1 cut(s) 228
AjuI GAANNNNNNNTTGG 2 cut(s) 993, 1025
AluBI AGCT 5 cut(s) 98, 206, 737, 833, 935
AluI AGCT 5 cut(s) 98, 206, 737, 833, 935
Alw26I GTCTC 1 cut(s) 66
AlwI GGATC 3 cut(s) 132, 160, 254
AoxI GGCC 5 cut(s) 110, 572, 642, 660, 906
ApeKI GCWGC 3 cut(s) 611, 752, 914
ApoI RAATTY 2 cut(s) 424, 1008
Asp718I GGTACC 1 cut(s) 1032
AspLEI GCGC 4 cut(s) 85, 223, 700, 730
AspS9I GGNCC 4 cut(s) 110, 414, 572, 907
AsuHPI GGTGA 9 cut(s) 18, 125, 170, 269, 275, 296, 329, 550, 989
AsuII TTCGAA 2 cut(s) 422, 1012
AsuNHI GCTAGC 1 cut(s) 94
AvaII GGWCC 1 cut(s) 414
BanI GGYRCC 2 cut(s) 880, 1032
BanII GRGCYC 4 cut(s) 467, 896, 923, 950
BbvI GCAGC 3 cut(s) 623, 739, 901
BccI CCATC 8 cut(s) 275, 520, 563, 630, 820, 868, 983, 1013
BceAI ACGGC 1 cut(s) 803
BcgI CGANNNNNNTGC 2 cut(s) 579, 613
BcoDI GTCTC 1 cut(s) 66
BfaI CTAG 3 cut(s) 95, 203, 848
BfmI CTRYAG 2 cut(s) 609, 828
BfoI RGCGCY 3 cut(s) 224, 701, 731
BglI GCCNNNNNGGC 1 cut(s) 109
BisI GCNGC 4 cut(s) 92, 612, 753, 915
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 4 cut(s) 93, 613, 754, 916
Bme18I GGWCC 1 cut(s) 414
BmgBI CACGTC 1 cut(s) 228
BmgT120I GGNCC 4 cut(s) 110, 414, 572, 907
BmiI GGNNCC 7 cut(s) 185, 415, 573, 804, 882, 909, 1034
BmsI GCATC 3 cut(s) 491, 739, 833
BmtI GCTAGC 1 cut(s) 98
BoxI GACNNNNGTC 1 cut(s) 404
BplI GAGNNNNNCTC 2 cut(s) 937, 969
BpmI CTGGAG 1 cut(s) 915
Bpu1102I GCTNAGC 1 cut(s) 99
Bpu14I TTCGAA 2 cut(s) 422, 1012
BsaJI CCNNGG 3 cut(s) 216, 477, 969
BsaWI WCCGGW 2 cut(s) 180, 877
BsaXI ACNNNNNCTCC 4 cut(s) 297, 327, 493, 523
Bsc4I CCNNNNNNNGG 2 cut(s) 904, 970
Bse1I ACTGG 1 cut(s) 593
BseDI CCNNGG 3 cut(s) 216, 477, 969
BseGI GGATG 2 cut(s) 582, 760
BseLI CCNNNNNNNGG 2 cut(s) 904, 970
BseMII CTCAG 3 cut(s) 113, 398, 933
BseNI ACTGG 1 cut(s) 593
BseRI GAGGAG 7 cut(s) 25, 471, 699, 729, 780, 864, 945
BseXI GCAGC 3 cut(s) 623, 739, 901
BseYI CCCAGC 3 cut(s) 190, 300, 549
BsgI GTGCAG 1 cut(s) 573
Bsh1236I CGCG 1 cut(s) 244
BshFI GGCC 5 cut(s) 112, 574, 644, 662, 908
BshNI GGYRCC 2 cut(s) 880, 1032
BsiSI CCGG 5 cut(s) 157, 181, 429, 878, 905
BslI CCNNNNNNNGG 2 cut(s) 904, 970
BsmAI GTCTC 1 cut(s) 66
BsmI GAATGC 1 cut(s) 152
BsnI GGCC 5 cut(s) 112, 574, 644, 662, 908
Bsp119I TTCGAA 2 cut(s) 422, 1012
Bsp1286I GDGCHC 4 cut(s) 467, 896, 923, 950
Bsp143I GATC 6 cut(s) 137, 165, 199, 246, 702, 1026
Bsp1720I GCTNAGC 1 cut(s) 99
Bsp19I CCATGG 1 cut(s) 969
BspACI CCGC 6 cut(s) 91, 146, 232, 244, 370, 619
BspANI GGCC 5 cut(s) 112, 574, 644, 662, 908
BspCNI CTCAG 3 cut(s) 112, 397, 934
BspFNI CGCG 1 cut(s) 244
BspLI GGNNCC 7 cut(s) 185, 415, 573, 804, 882, 909, 1034
BspMAI CTGCAG 1 cut(s) 613
BspOI GCTAGC 1 cut(s) 98
BspPI GGATC 3 cut(s) 132, 160, 254
BspT104I TTCGAA 2 cut(s) 422, 1012
BspT107I GGYRCC 2 cut(s) 880, 1032
BsrI ACTGG 1 cut(s) 593
BssECI CCNNGG 3 cut(s) 216, 477, 969
BssMI GATC 6 cut(s) 137, 165, 199, 246, 702, 1026
BssT1I CCWWGG 2 cut(s) 216, 969
Bst4CI ACNGT 3 cut(s) 79, 403, 606
Bst6I CTCTTC 1 cut(s) 315
BstBI TTCGAA 2 cut(s) 422, 1012
BstC8I GCNNGC 6 cut(s) 96, 150, 234, 531, 774, 919
BstDEI CTNAG 4 cut(s) 99, 210, 384, 942
BstDSI CCRYGG 1 cut(s) 969
BstF5I GGATG 2 cut(s) 582, 760
BstFNI CGCG 1 cut(s) 244
BstH2I RGCGCY 3 cut(s) 224, 701, 731
BstHHI GCGC 4 cut(s) 85, 223, 700, 730
BstKTI GATC 6 cut(s) 140, 168, 202, 249, 705, 1029
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 6 cut(s) 137, 165, 199, 246, 702, 1026
BstMWI GCNNNNNNNGC 8 cut(s) 91, 100, 109, 229, 376, 734, 830, 914
BstPAI GACNNNNGTC 1 cut(s) 404
BstSFI CTRYAG 2 cut(s) 609, 828
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 3 cut(s) 623, 739, 901
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BsuRI GGCC 5 cut(s) 112, 574, 644, 662, 908
BtgI CCRYGG 1 cut(s) 969
BtgZI GCGATG 2 cut(s) 510, 608
BtrI CACGTC 1 cut(s) 228
BtsCI GGATG 2 cut(s) 582, 760
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 4 cut(s) 75, 339, 408, 602
Cac8I GCNNGC 6 cut(s) 96, 150, 234, 531, 774, 919
CfoI GCGC 4 cut(s) 85, 223, 700, 730
Cfr13I GGNCC 4 cut(s) 110, 414, 572, 907
CseI GACGC 3 cut(s) 627, 645, 657
Csp6I GTAC 1 cut(s) 1033
CspCI CAANNNNNGTGG 2 cut(s) 754, 789
CviAII CATG 1 cut(s) 970
CviQI GTAC 1 cut(s) 1033
DdeI CTNAG 4 cut(s) 99, 210, 384, 942
DpnI GATC 6 cut(s) 139, 167, 201, 248, 704, 1028
DpnII GATC 6 cut(s) 137, 165, 199, 246, 702, 1026
EaeI YGGCCR 2 cut(s) 642, 660
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco130I CCWWGG 2 cut(s) 216, 969
Eco24I GRGCYC 4 cut(s) 467, 896, 923, 950
Eco32I GATATC 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 414
EcoO109I RGGNCCY 1 cut(s) 572
EcoRI GAATTC 2 cut(s) 424, 1008
EcoRV GATATC 1 cut(s) 390
EcoT14I CCWWGG 2 cut(s) 216, 969
EcoT38I GRGCYC 4 cut(s) 467, 896, 923, 950
ErhI CCWWGG 2 cut(s) 216, 969
FaeI CATG 1 cut(s) 973
FaiI YATR 4 cut(s) 42, 647, 665, 971
FatI CATG 1 cut(s) 969
FauI CCCGC 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 92, 612, 753, 915
FokI GGATG 2 cut(s) 569, 747
FriOI GRGCYC 4 cut(s) 467, 896, 923, 950
Fsp4HI GCNGC 4 cut(s) 92, 612, 753, 915
FspBI CTAG 3 cut(s) 95, 203, 848
FspI TGCGCA 1 cut(s) 84
GlaI GCGC 4 cut(s) 84, 222, 699, 729
GluI GCNGC 4 cut(s) 92, 612, 753, 915
GsaI CCCAGC 3 cut(s) 194, 304, 553
GsuI CTGGAG 1 cut(s) 915
HaeII RGCGCY 3 cut(s) 224, 701, 731
HaeIII GGCC 5 cut(s) 112, 574, 644, 662, 908
HapII CCGG 5 cut(s) 157, 181, 429, 878, 905
HgaI GACGC 3 cut(s) 627, 645, 657
HhaI GCGC 4 cut(s) 85, 223, 700, 730
Hin1II CATG 1 cut(s) 973
Hin6I GCGC 4 cut(s) 83, 221, 698, 728
HinP1I GCGC 4 cut(s) 83, 221, 698, 728
HinfI GANTC 6 cut(s) 266, 287, 293, 382, 419, 981
HpaII CCGG 5 cut(s) 157, 181, 429, 878, 905
HphI GGTGA 9 cut(s) 18, 125, 170, 269, 275, 296, 329, 550, 989
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 8 cut(s) 165, 271, 292, 387, 418, 496, 690, 986
Hpy188III TCNNGA 2 cut(s) 443, 718
Hpy8I GTNNAC 1 cut(s) 133
Hpy99I CGWCG 4 cut(s) 400, 643, 661, 859
HpyAV CCTTC 7 cut(s) 33, 254, 346, 571, 585, 792, 936
HpyCH4III ACNGT 3 cut(s) 79, 403, 606
HpyCH4IV ACGT 1 cut(s) 227
HpyCH4V TGCA 4 cut(s) 152, 590, 611, 824
HpyF10VI GCNNNNNNNGC 8 cut(s) 91, 100, 109, 229, 376, 734, 830, 914
HpyF3I CTNAG 4 cut(s) 99, 210, 384, 942
HpySE526I ACGT 1 cut(s) 227
Hsp92II CATG 1 cut(s) 973
HspAI GCGC 4 cut(s) 83, 221, 698, 728
KpnI GGTACC 1 cut(s) 1036
Kzo9I GATC 6 cut(s) 137, 165, 199, 246, 702, 1026
LmnI GCTCC 3 cut(s) 183, 742, 940
Lsp1109I GCAGC 3 cut(s) 623, 739, 901
LweI GCATC 3 cut(s) 491, 739, 833
MaeI CTAG 3 cut(s) 95, 203, 848
MaeII ACGT 1 cut(s) 227
MaeIII GTNAC 3 cut(s) 538, 600, 973
MalI GATC 6 cut(s) 139, 167, 201, 248, 704, 1028
MboI GATC 6 cut(s) 137, 165, 199, 246, 702, 1026
MboII GAAGA 5 cut(s) 290, 332, 338, 373, 376
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 4 cut(s) 467, 896, 923, 950
MluCI AATT 2 cut(s) 424, 1008
MlyI GAGTC 2 cut(s) 376, 975
MmeI TCCRAC 2 cut(s) 320, 713
MspA1I CMGCKG 1 cut(s) 91
MspI CCGG 5 cut(s) 157, 181, 429, 878, 905
Mva1269I GAATGC 1 cut(s) 152
MvnI CGCG 1 cut(s) 244
MwoI GCNNNNNNNGC 8 cut(s) 91, 100, 109, 229, 376, 734, 830, 914
NcoI CCATGG 1 cut(s) 969
NdeII GATC 6 cut(s) 137, 165, 199, 246, 702, 1026
NheI GCTAGC 1 cut(s) 94
NlaIII CATG 1 cut(s) 973
NlaIV GGNNCC 7 cut(s) 185, 415, 573, 804, 882, 909, 1034
NmeAIII GCCGAG 1 cut(s) 458
NmuCI GTSAC 3 cut(s) 538, 600, 973
NsbI TGCGCA 1 cut(s) 84
NspV TTCGAA 2 cut(s) 422, 1012
PcsI WCGNNNNNNNCGW 3 cut(s) 233, 414, 665
PctI GAATGC 1 cut(s) 152
PfeI GAWTC 4 cut(s) 266, 287, 293, 419
PflFI GACNNNGTC 1 cut(s) 637
PflMI CCANNNNNTGG 1 cut(s) 970
PkrI GCNGC 4 cut(s) 93, 613, 754, 916
PleI GAGTC 2 cut(s) 376, 975
PpsI GAGTC 2 cut(s) 376, 975
PshAI GACNNNNGTC 1 cut(s) 404
PspFI CCCAGC 3 cut(s) 190, 300, 549
PspN4I GGNNCC 7 cut(s) 185, 415, 573, 804, 882, 909, 1034
PspPI GGNCC 4 cut(s) 110, 414, 572, 907
PstI CTGCAG 1 cut(s) 613
PsuI RGATCY 1 cut(s) 246
PsyI GACNNNGTC 1 cut(s) 637
RsaI GTAC 1 cut(s) 1034
RsaNI GTAC 1 cut(s) 1033
SatI GCNGC 4 cut(s) 92, 612, 753, 915
Sau3AI GATC 6 cut(s) 137, 165, 199, 246, 702, 1026
Sau96I GGNCC 4 cut(s) 110, 414, 572, 907
SchI GAGTC 2 cut(s) 376, 975
SduI GDGCHC 4 cut(s) 467, 896, 923, 950
SfaNI GCATC 3 cut(s) 491, 739, 833
SfcI CTRYAG 2 cut(s) 609, 828
SfuI TTCGAA 2 cut(s) 422, 1012
SinI GGWCC 1 cut(s) 414
Sse9I AATT 2 cut(s) 424, 1008
SsiI CCGC 6 cut(s) 91, 146, 232, 244, 370, 619
SspMI CTAG 3 cut(s) 95, 203, 848
StyI CCWWGG 2 cut(s) 216, 969
TaaI ACNGT 3 cut(s) 79, 403, 606
TaiI ACGT 1 cut(s) 230
TaqI TCGA 3 cut(s) 68, 422, 1012
TasI AATT 2 cut(s) 424, 1008
TauI GCSGC 1 cut(s) 94
TfiI GAWTC 4 cut(s) 266, 287, 293, 419
TscAI CASTG 4 cut(s) 82, 339, 408, 609
TseFI GTSAC 3 cut(s) 538, 600, 973
TseI GCWGC 3 cut(s) 611, 752, 914
Tsp45I GTSAC 3 cut(s) 538, 600, 973
TspRI CASTG 4 cut(s) 82, 339, 408, 609
Tth111I GACNNNGTC 1 cut(s) 637
Van91I CCANNNNNTGG 1 cut(s) 970
VpaK11BI GGWCC 1 cut(s) 414
XapI RAATTY 2 cut(s) 424, 1008
XspI CTAG 3 cut(s) 95, 203, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.